FTSJ3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FTSJ3
Entrez Gene ID	117246
Full Name	FtsJ RNA methyltransferase homolog 3
Synonyms	EPCS3,SPB1
General protein information	Preferred Names FtsJ RNA methyltransferase homolog 3 Names pre-rRNA processing protein FTSJ3 2'-O-ribose RNA methyltransferase SPB1 homolog FtsJ homolog 3 SPB1 RNA methyltransferase homolog protein ftsJ homolog 3 putative rRNA methyltransferase 3 rRNA (uridine-2'-O-)-methyltransferase 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.3
Summary	Although the function of this gene is not known, the existence of this gene is supported by mRNA and EST data. A possible function of the encoded protein can be inferred from amino acid sequence similarity to the E.coli FtsJ protein and to a mouse protein possibly involved in embryogenesis. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017647.4	NP_060117.3	pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
NM_017647.3	NP_060117.3	pre-rRNA processing protein FTSJ3
XM_024450562.1	XP_024306330.1	pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FTSJ3	NP_060117.3
Canis lupus familiaris (dog)	FTSJ3	XP_005624307.1
Rattus norvegicus (Norway rat)	Ftsj3	NP_001012014.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003002	XP_311882.5
Xenopus tropicalis (tropical clawed frog)	ftsj3	NP_001072428.1
Gallus gallus (chicken)	FTSJ3	NP_001026096.1
Drosophila melanogaster (fruit fly)	CG8939	NP_573099.1
Pan troglodytes (chimpanzee)	FTSJ3	XP_003315725.1
Bos taurus (cattle)	FTSJ3	NP_001068830.1
Mus musculus (house mouse)	Ftsj3	NP_079586.1
Danio rerio (zebrafish)	ftsj	XP_002666179.1
	FTSJ3	XP_001116413.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Components of the FTSJ3 interactome identified by coimmunoprecipitation.
Title: Proteomic characterization of the human FTSJ3 preribosomal complexes.

The results presented in this work indicate a close functional interaction between NIP7 and FTSJ3 during pre-rRNA processing and show that FTSJ3 participates in ribosome synthesis in human cells.
Title: The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing.

The following FTSJ3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FTSJ3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104243	XM_024450562.1 Latest version!	Homo sapiens FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu02482	NM_017647.4 Latest version!	Homo sapiens FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu02482	NM_017647.3	Homo sapiens FtsJ RNA methyltransferase homolog 3 (FTSJ3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FTSJ3 ( XM_024450562.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306330.1 Homo sapiens FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3), transcript variant X1, mRNA.

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CloneID	OHu104243
Clone ID Related Accession (Same CDS sequence)	XM_024450562.1
Accession Version	XM_024450562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAGA AGGGCAAAGT TGGCAAGAGC CGACGAGACA AGTTTTATCA CTTGGCGAAG 
GAGACGGGTT ACCGTTCCCG ATCTGCTTTC AAGCTGATCC AGCTCAATCG CCGCTTTCAG 
TTCCTGCAGA AAGCCCGAGC CTTGCTGGAC CTGTGTGCTG CGCCAGGGGG ATGGCTGCAG 
GTAGCTGCCA AGTTTATGCC TGTATCCAGC CTTATTGTGG GAGTGGACCT GGTTCCAATC 
AAGCCTCTCC CCAATGTGGT GACTCTCCAG CAGGACATCA CAACAGAACG TTGTAGGCAG 
GCCCTGAGGA AGGAGCTGAA GACCTGGAAG GTTGATGTTG TGCTCAATGA TGGGGCCCCC 
AACGTTGGGG CTAGCTGGGT CCATGATGCT TACTCACAAG CCCATTTGAC ACTGATGGCT 
CTACGTTTGG CTTGTGACTT TTTGGCCCGT GGTGGCAGCT TCATCACAAA GGTTTTCCGT 
TCTCGTGACT ATCAGCCTCT GCTATGGATC TTTCAGCAGC TGTTCCGCCG TGTCCAGGCC 
ACCAAGCCCC AAGCCTCTCG CCATGAATCT GCAGAGATCT TTGTAGTCTG CCAAGGATTC 
CTGGCCCCTG ACAAGGTTGA CAGTAAATTC TTTGACCCCA AATTTGCCTT TAAGGAGGTT 
GAAGTTCAGG CTAAGACCGT TACTGAATTG GTTACTAAGA AGAAGCCAAA GGCTGAAGGC 
TATGCTGAGG GTGACCTCAC TCTCTATCAC CGTACCTCAG TCACTGACTT CCTCCGAGCT 
GCCAACCCTG TTGACTTCCT CTCCAAGGCC AGCGAAATCA TGGTAGATGA TGAAGAGTTG 
GCACAGCATC CAGCTACCAC TGAGGACATA CGGGTGTGCT GTCAGGACAT CAGAGTGTTG 
GGGCGCAAGG AGCTCAGGTC GCTACTAAAC TGGAGAACAA AACTTCGGCG ATATGTGGCC 
AAGAAGCTGA AAGAACAAGC AAAGGCACTG GACATCAGCC TCAGCTCTGG AGAGGAAGAT 
GAAGGTGATG AGGAGGACTC AACAGCTGGA ACCACAAAGC AGCCCTCTAA GGAGGAGGAG 
GAAGAGGAGG AGGAGGAACA ACTGAACCAG ACCTTGGCAG AAATGAAGGC CCAGGAGGTG 
GCGGAATTGA AGAGGAAGAA AAAGAAGCTG TTGCGTGAGC AGAGAAAGCA GCGGGAGCGT 
GTGGAGCTGA AGATGGATCT GCCTGGGGTT TCCATTGCAG ACGAGGGGGA GACTGGCATG 
TTCTCCTTGA GCACCATCCG GGGTCACCAG GCTGGAGTTC AGTGGTGCCA TCATGACTCA 
CTGCAGCCTC AAACTCCTGG CATCAAGCAA TCCTCCTGCC TCAGCCTCCC AAAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306330.1
CDS	74..1453
Translation	MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVGVDLVPIKPLPNVVTLQQDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGSFITKVFRSRDYQPLLWIFQQLFRRVQATKPQASRHESAEIFVVCQGFLAPDKVDSKFFDPKFAFKEVEVQAKTVTELVTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEIMVDDEELAQHPATTEDIRVCCQDIRVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSGEEDEGDEEDSTAGTTKQPSKEEEEEEEEEQLNQTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFSLSTIRGHQAGVQWCHHDSLQPQTPGIKQSSCLSLPKC

Target ORF information:

RefSeq Version	XM_024450562.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450562.1

ORF Insert Sequence:

ATGGGCAAGA AGGGCAAAGT TGGCAAGAGC CGACGAGACA AGTTTTATCA CTTGGCGAAG 
GAGACGGGTT ACCGTTCCCG ATCTGCTTTC AAGCTGATCC AGCTCAATCG CCGCTTTCAG 
TTCCTGCAGA AAGCCCGAGC CTTGCTGGAC CTGTGTGCTG CGCCAGGGGG ATGGCTGCAG 
GTAGCTGCCA AGTTTATGCC TGTATCCAGC CTTATTGTGG GAGTGGACCT GGTTCCAATC 
AAGCCTCTCC CCAATGTGGT GACTCTCCAG CAGGACATCA CAACAGAACG TTGTAGGCAG 
GCCCTGAGGA AGGAGCTGAA GACCTGGAAG GTTGATGTTG TGCTCAATGA TGGGGCCCCC 
AACGTTGGGG CTAGCTGGGT CCATGATGCT TACTCACAAG CCCATTTGAC ACTGATGGCT 
CTACGTTTGG CTTGTGACTT TTTGGCCCGT GGTGGCAGCT TCATCACAAA GGTTTTCCGT 
TCTCGTGACT ATCAGCCTCT GCTATGGATC TTTCAGCAGC TGTTCCGCCG TGTCCAGGCC 
ACCAAGCCCC AAGCCTCTCG CCATGAATCT GCAGAGATCT TTGTAGTCTG CCAAGGATTC 
CTGGCCCCTG ACAAGGTTGA CAGTAAATTC TTTGACCCCA AATTTGCCTT TAAGGAGGTT 
GAAGTTCAGG CTAAGACCGT TACTGAATTG GTTACTAAGA AGAAGCCAAA GGCTGAAGGC 
TATGCTGAGG GTGACCTCAC TCTCTATCAC CGTACCTCAG TCACTGACTT CCTCCGAGCT 
GCCAACCCTG TTGACTTCCT CTCCAAGGCC AGCGAAATCA TGGTAGATGA TGAAGAGTTG 
GCACAGCATC CAGCTACCAC TGAGGACATA CGGGTGTGCT GTCAGGACAT CAGAGTGTTG 
GGGCGCAAGG AGCTCAGGTC GCTACTAAAC TGGAGAACAA AACTTCGGCG ATATGTGGCC 
AAGAAGCTGA AAGAACAAGC AAAGGCACTG GACATCAGCC TCAGCTCTGG AGAGGAAGAT 
GAAGGTGATG AGGAGGACTC AACAGCTGGA ACCACAAAGC AGCCCTCTAA GGAGGAGGAG 
GAAGAGGAGG AGGAGGAACA ACTGAACCAG ACCTTGGCAG AAATGAAGGC CCAGGAGGTG 
GCGGAATTGA AGAGGAAGAA AAAGAAGCTG TTGCGTGAGC AGAGAAAGCA GCGGGAGCGT 
GTGGAGCTGA AGATGGATCT GCCTGGGGTT TCCATTGCAG ACGAGGGGGA GACTGGCATG 
TTCTCCTTGA GCACCATCCG GGGTCACCAG GCTGGAGTTC AGTGGTGCCA TCATGACTCA 
CTGCAGCCTC AAACTCCTGG CATCAAGCAA TCCTCCTGCC TCAGCCTCCC AAAATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTSJ3 ( NM_017647.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060117.3 Homo sapiens FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3), mRNA.

Price & Availability↑

CloneID	OHu02482
Clone ID Related Accession (Same CDS sequence)	NM_017647.4 , NM_017647.3
Accession Version	NM_017647.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095861.1, AK315010.1, AF147378.1 and BM914774.1. On Nov 23, 2018 this sequence version replaced NM_017647.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.15384.1, SRR1660803.125988.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000427159.7/ ENSP00000396673.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAAGA AGGGCAAAGT TGGCAAGAGC CGACGAGACA AGTTTTATCA CTTGGCGAAG 
GAGACGGGTT ACCGTTCCCG ATCTGCTTTC AAGCTGATCC AGCTCAATCG CCGCTTTCAG 
TTCCTGCAGA AAGCCCGAGC CTTGCTGGAC CTGTGTGCTG CGCCAGGGGG ATGGCTGCAG 
GTAGCTGCCA AGTTTATGCC TGTATCCAGC CTTATTGTGG GAGTGGACCT GGTTCCAATC 
AAGCCTCTCC CCAATGTGGT GACTCTCCAG CAGGACATCA CAACAGAACG TTGTAGGCAG 
GCCCTGAGGA AGGAGCTGAA GACCTGGAAG GTTGATGTTG TGCTCAATGA TGGGGCCCCC 
AACGTTGGGG CTAGCTGGGT CCATGATGCT TACTCACAAG CCCATTTGAC ACTGATGGCT 
CTACGTTTGG CTTGTGACTT TTTGGCCCGT GGTGGCAGCT TCATCACAAA GGTTTTCCGT 
TCTCGTGACT ATCAGCCTCT GCTATGGATC TTTCAGCAGC TGTTCCGCCG TGTCCAGGCC 
ACCAAGCCCC AAGCCTCTCG CCATGAATCT GCAGAGATCT TTGTAGTCTG CCAAGGATTC 
CTGGCCCCTG ACAAGGTTGA CAGTAAATTC TTTGACCCCA AATTTGCCTT TAAGGAGGTT 
GAAGTTCAGG CTAAGACCGT TACTGAATTG GTTACTAAGA AGAAGCCAAA GGCTGAAGGC 
TATGCTGAGG GTGACCTCAC TCTCTATCAC CGTACCTCAG TCACTGACTT CCTCCGAGCT 
GCCAACCCTG TTGACTTCCT CTCCAAGGCC AGCGAAATCA TGGTAGATGA TGAAGAGTTG 
GCACAGCATC CAGCTACCAC TGAGGACATA CGGGTGTGCT GTCAGGACAT CAGAGTGTTG 
GGGCGCAAGG AGCTCAGGTC GCTACTAAAC TGGAGAACAA AACTTCGGCG ATATGTGGCC 
AAGAAGCTGA AAGAACAAGC AAAGGCACTG GACATCAGCC TCAGCTCTGG AGAGGAAGAT 
GAAGGTGATG AGGAGGACTC AACAGCTGGA ACCACAAAGC AGCCCTCTAA GGAGGAGGAG 
GAAGAGGAGG AGGAGGAACA ACTGAACCAG ACCTTGGCAG AAATGAAGGC CCAGGAGGTG 
GCGGAATTGA AGAGGAAGAA AAAGAAGCTG TTGCGTGAGC AGAGAAAGCA GCGGGAGCGT 
GTGGAGCTGA AGATGGATCT GCCTGGGGTT TCCATTGCAG ACGAGGGGGA GACTGGCATG 
TTCTCCTTGA GCACCATCCG GGGTCACCAG TTATTAGAGG AAGTAACACA AGGGGATATG 
AGTGCAGCAG ACACATTTCT GTCCGATCTG CCAAGGGATG ATATCTATGT GTCAGATGTT 
GAGGACGACG GTGATGACAC ATCTCTGGAT AGTGACCTGG ATCCAGAGGA GCTGGCAGGA 
GTCAGGGGAC ATCAGGGTCT AAGGGACCAA AAGCGTATGC GACTTACTGA AGTGCAAGAT 
GATAAAGAGG AGGAGGAGGA GGAGAATCCA CTGCTGGTAC CACTGGAGGA AAAGGCAGTA 
CTGCAGGAAG AACAAGCCAA CCTGTGGTTC TCAAAGGGCA GCTTTGCTGG GATCGAGGAC 
GATGCCGATG AGGCCCTGGA GATCAGTCAG GCCCAGCTGT TATTTGAGAA CCGGCGGAAG 
GGACGGCAGC AGCAGCAGAA GCAGCAGCTG CCACAGACAC CCCCTTCCTG TTTGAAGACT 
GAGATAATGT CTCCCCTGTA CCAAGATGAA GCCCCTAAGG GAACAGAGGC TTCTTCGGGG 
ACAGAAGCTG CCACTGGCCT TGAAGGGGAA GAAAAGGATG GCATCTCAGA CAGTGATAGC 
AGTACTAGCA GTGAGGAAGA AGAGAGCTGG GAACCCCTCC GTGGTAAGAA GCGAAGCCGT 
GGGCCTAAGT CAGATGATGA CGGGTTTGAG ATAGTGCCTA TTGAGGACCC AGCGAAACAT 
CGGATACTGG ACCCCGAAGG CCTTGCTCTA GGTGCTGTTA TTGCCTCTTC CAAAAAGGCC 
AAGAGAGACC TCATAGATAA CTCCTTCAAC CGGTACACAT TTAATGAGGA TGAGGGGGAG 
CTTCCGGAGT GGTTTGTGCA AGAGGAAAAG CAGCACCGGA TACGACAGTT GCCTGTTGGT 
AAGAAGGAGG TGGAGCATTA CCGGAAACGC TGGCGGGAAA TCAATGCACG TCCCATCAAG 
AAGGTGGCTG AGGCTAAGGC TAGAAAGAAA AGGAGGATGC TGAAGAGGCT GGAGCAGACC 
AGGAAGAAGG CAGAAGCCGT GGTGAACACA GTGGACATCT CAGAACGAGA GAAAGTGGCA 
CAGCTGCGAA GTCTCTACAA GAAGGCTGGG CTTGGCAAGG AGAAACGCCA TGTCACCTAC 
GTTGTAGCCA AAAAAGGTGT GGGCCGCAAA GTGCGCCGGC CAGCTGGAGT CAGAGGTCAT 
TTCAAGGTGG TGGACTCAAG GATGAAGAAG GACCAAAGAG CACAGCAACG TAAGGAACAA 
AAGAAAAAAC ACAAACGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060117.3
CDS	639..3182
Translation	MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVGVDLVPIKPLPNVVTLQQDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGSFITKVFRSRDYQPLLWIFQQLFRRVQATKPQASRHESAEIFVVCQGFLAPDKVDSKFFDPKFAFKEVEVQAKTVTELVTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEIMVDDEELAQHPATTEDIRVCCQDIRVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSGEEDEGDEEDSTAGTTKQPSKEEEEEEEEEQLNQTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFSLSTIRGHQLLEEVTQGDMSAADTFLSDLPRDDIYVSDVEDDGDDTSLDSDLDPEELAGVRGHQGLRDQKRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK

Target ORF information:

RefSeq Version	NM_017647.4
Organism	Homo sapiens(human)
Definition	Homo sapiens FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017647.4

ORF Insert Sequence:

ATGGGCAAGA AGGGCAAAGT TGGCAAGAGC CGACGAGACA AGTTTTATCA CTTGGCGAAG 
GAGACGGGTT ACCGTTCCCG ATCTGCTTTC AAGCTGATCC AGCTCAATCG CCGCTTTCAG 
TTCCTGCAGA AAGCCCGAGC CTTGCTGGAC CTGTGTGCTG CGCCAGGGGG ATGGCTGCAG 
GTAGCTGCCA AGTTTATGCC TGTATCCAGC CTTATTGTGG GAGTGGACCT GGTTCCAATC 
AAGCCTCTCC CCAATGTGGT GACTCTCCAG CAGGACATCA CAACAGAACG TTGTAGGCAG 
GCCCTGAGGA AGGAGCTGAA GACCTGGAAG GTTGATGTTG TGCTCAATGA TGGGGCCCCC 
AACGTTGGGG CTAGCTGGGT CCATGATGCT TACTCACAAG CCCATTTGAC ACTGATGGCT 
CTACGTTTGG CTTGTGACTT TTTGGCCCGT GGTGGCAGCT TCATCACAAA GGTTTTCCGT 
TCTCGTGACT ATCAGCCTCT GCTATGGATC TTTCAGCAGC TGTTCCGCCG TGTCCAGGCC 
ACCAAGCCCC AAGCCTCTCG CCATGAATCT GCAGAGATCT TTGTAGTCTG CCAAGGATTC 
CTGGCCCCTG ACAAGGTTGA CAGTAAATTC TTTGACCCCA AATTTGCCTT TAAGGAGGTT 
GAAGTTCAGG CTAAGACCGT TACTGAATTG GTTACTAAGA AGAAGCCAAA GGCTGAAGGC 
TATGCTGAGG GTGACCTCAC TCTCTATCAC CGTACCTCAG TCACTGACTT CCTCCGAGCT 
GCCAACCCTG TTGACTTCCT CTCCAAGGCC AGCGAAATCA TGGTAGATGA TGAAGAGTTG 
GCACAGCATC CAGCTACCAC TGAGGACATA CGGGTGTGCT GTCAGGACAT CAGAGTGTTG 
GGGCGCAAGG AGCTCAGGTC GCTACTAAAC TGGAGAACAA AACTTCGGCG ATATGTGGCC 
AAGAAGCTGA AAGAACAAGC AAAGGCACTG GACATCAGCC TCAGCTCTGG AGAGGAAGAT 
GAAGGTGATG AGGAGGACTC AACAGCTGGA ACCACAAAGC AGCCCTCTAA GGAGGAGGAG 
GAAGAGGAGG AGGAGGAACA ACTGAACCAG ACCTTGGCAG AAATGAAGGC CCAGGAGGTG 
GCGGAATTGA AGAGGAAGAA AAAGAAGCTG TTGCGTGAGC AGAGAAAGCA GCGGGAGCGT 
GTGGAGCTGA AGATGGATCT GCCTGGGGTT TCCATTGCAG ACGAGGGGGA GACTGGCATG 
TTCTCCTTGA GCACCATCCG GGGTCACCAG TTATTAGAGG AAGTAACACA AGGGGATATG 
AGTGCAGCAG ACACATTTCT GTCCGATCTG CCAAGGGATG ATATCTATGT GTCAGATGTT 
GAGGACGACG GTGATGACAC ATCTCTGGAT AGTGACCTGG ATCCAGAGGA GCTGGCAGGA 
GTCAGGGGAC ATCAGGGTCT AAGGGACCAA AAGCGTATGC GACTTACTGA AGTGCAAGAT 
GATAAAGAGG AGGAGGAGGA GGAGAATCCA CTGCTGGTAC CACTGGAGGA AAAGGCAGTA 
CTGCAGGAAG AACAAGCCAA CCTGTGGTTC TCAAAGGGCA GCTTTGCTGG GATCGAGGAC 
GATGCCGATG AGGCCCTGGA GATCAGTCAG GCCCAGCTGT TATTTGAGAA CCGGCGGAAG 
GGACGGCAGC AGCAGCAGAA GCAGCAGCTG CCACAGACAC CCCCTTCCTG TTTGAAGACT 
GAGATAATGT CTCCCCTGTA CCAAGATGAA GCCCCTAAGG GAACAGAGGC TTCTTCGGGG 
ACAGAAGCTG CCACTGGCCT TGAAGGGGAA GAAAAGGATG GCATCTCAGA CAGTGATAGC 
AGTACTAGCA GTGAGGAAGA AGAGAGCTGG GAACCCCTCC GTGGTAAGAA GCGAAGCCGT 
GGGCCTAAGT CAGATGATGA CGGGTTTGAG ATAGTGCCTA TTGAGGACCC AGCGAAACAT 
CGGATACTGG ACCCCGAAGG CCTTGCTCTA GGTGCTGTTA TTGCCTCTTC CAAAAAGGCC 
AAGAGAGACC TCATAGATAA CTCCTTCAAC CGGTACACAT TTAATGAGGA TGAGGGGGAG 
CTTCCGGAGT GGTTTGTGCA AGAGGAAAAG CAGCACCGGA TACGACAGTT GCCTGTTGGT 
AAGAAGGAGG TGGAGCATTA CCGGAAACGC TGGCGGGAAA TCAATGCACG TCCCATCAAG 
AAGGTGGCTG AGGCTAAGGC TAGAAAGAAA AGGAGGATGC TGAAGAGGCT GGAGCAGACC 
AGGAAGAAGG CAGAAGCCGT GGTGAACACA GTGGACATCT CAGAACGAGA GAAAGTGGCA 
CAGCTGCGAA GTCTCTACAA GAAGGCTGGG CTTGGCAAGG AGAAACGCCA TGTCACCTAC 
GTTGTAGCCA AAAAAGGTGT GGGCCGCAAA GTGCGCCGGC CAGCTGGAGT CAGAGGTCAT 
TTCAAGGTGG TGGACTCAAG GATGAAGAAG GACCAAAGAG CACAGCAACG TAAGGAACAA 
AAGAAAAAAC ACAAACGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTSJ3 ( NM_017647.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060117.3 Homo sapiens FtsJ RNA methyltransferase homolog 3 (FTSJ3), mRNA.

Price & Availability↑

CloneID	OHu02482
Clone ID Related Accession (Same CDS sequence)	NM_017647.4 , NM_017647.3
Accession Version	NM_017647.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	pre-rRNA processing protein FTSJ3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095861.1, AK315010.1, AF147378.1 and BM914774.1. On Jul 11, 2008 this sequence version replaced NM_017647.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.15384.1, SRR1660803.125988.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAAGA AGGGCAAAGT TGGCAAGAGC CGACGAGACA AGTTTTATCA CTTGGCGAAG 
GAGACGGGTT ACCGTTCCCG ATCTGCTTTC AAGCTGATCC AGCTCAATCG CCGCTTTCAG 
TTCCTGCAGA AAGCCCGAGC CTTGCTGGAC CTGTGTGCTG CGCCAGGGGG ATGGCTGCAG 
GTAGCTGCCA AGTTTATGCC TGTATCCAGC CTTATTGTGG GAGTGGACCT GGTTCCAATC 
AAGCCTCTCC CCAATGTGGT GACTCTCCAG CAGGACATCA CAACAGAACG TTGTAGGCAG 
GCCCTGAGGA AGGAGCTGAA GACCTGGAAG GTTGATGTTG TGCTCAATGA TGGGGCCCCC 
AACGTTGGGG CTAGCTGGGT CCATGATGCT TACTCACAAG CCCATTTGAC ACTGATGGCT 
CTACGTTTGG CTTGTGACTT TTTGGCCCGT GGTGGCAGCT TCATCACAAA GGTTTTCCGT 
TCTCGTGACT ATCAGCCTCT GCTATGGATC TTTCAGCAGC TGTTCCGCCG TGTCCAGGCC 
ACCAAGCCCC AAGCCTCTCG CCATGAATCT GCAGAGATCT TTGTAGTCTG CCAAGGATTC 
CTGGCCCCTG ACAAGGTTGA CAGTAAATTC TTTGACCCCA AATTTGCCTT TAAGGAGGTT 
GAAGTTCAGG CTAAGACCGT TACTGAATTG GTTACTAAGA AGAAGCCAAA GGCTGAAGGC 
TATGCTGAGG GTGACCTCAC TCTCTATCAC CGTACCTCAG TCACTGACTT CCTCCGAGCT 
GCCAACCCTG TTGACTTCCT CTCCAAGGCC AGCGAAATCA TGGTAGATGA TGAAGAGTTG 
GCACAGCATC CAGCTACCAC TGAGGACATA CGGGTGTGCT GTCAGGACAT CAGAGTGTTG 
GGGCGCAAGG AGCTCAGGTC GCTACTAAAC TGGAGAACAA AACTTCGGCG ATATGTGGCC 
AAGAAGCTGA AAGAACAAGC AAAGGCACTG GACATCAGCC TCAGCTCTGG AGAGGAAGAT 
GAAGGTGATG AGGAGGACTC AACAGCTGGA ACCACAAAGC AGCCCTCTAA GGAGGAGGAG 
GAAGAGGAGG AGGAGGAACA ACTGAACCAG ACCTTGGCAG AAATGAAGGC CCAGGAGGTG 
GCGGAATTGA AGAGGAAGAA AAAGAAGCTG TTGCGTGAGC AGAGAAAGCA GCGGGAGCGT 
GTGGAGCTGA AGATGGATCT GCCTGGGGTT TCCATTGCAG ACGAGGGGGA GACTGGCATG 
TTCTCCTTGA GCACCATCCG GGGTCACCAG TTATTAGAGG AAGTAACACA AGGGGATATG 
AGTGCAGCAG ACACATTTCT GTCCGATCTG CCAAGGGATG ATATCTATGT GTCAGATGTT 
GAGGACGACG GTGATGACAC ATCTCTGGAT AGTGACCTGG ATCCAGAGGA GCTGGCAGGA 
GTCAGGGGAC ATCAGGGTCT AAGGGACCAA AAGCGTATGC GACTTACTGA AGTGCAAGAT 
GATAAAGAGG AGGAGGAGGA GGAGAATCCA CTGCTGGTAC CACTGGAGGA AAAGGCAGTA 
CTGCAGGAAG AACAAGCCAA CCTGTGGTTC TCAAAGGGCA GCTTTGCTGG GATCGAGGAC 
GATGCCGATG AGGCCCTGGA GATCAGTCAG GCCCAGCTGT TATTTGAGAA CCGGCGGAAG 
GGACGGCAGC AGCAGCAGAA GCAGCAGCTG CCACAGACAC CCCCTTCCTG TTTGAAGACT 
GAGATAATGT CTCCCCTGTA CCAAGATGAA GCCCCTAAGG GAACAGAGGC TTCTTCGGGG 
ACAGAAGCTG CCACTGGCCT TGAAGGGGAA GAAAAGGATG GCATCTCAGA CAGTGATAGC 
AGTACTAGCA GTGAGGAAGA AGAGAGCTGG GAACCCCTCC GTGGTAAGAA GCGAAGCCGT 
GGGCCTAAGT CAGATGATGA CGGGTTTGAG ATAGTGCCTA TTGAGGACCC AGCGAAACAT 
CGGATACTGG ACCCCGAAGG CCTTGCTCTA GGTGCTGTTA TTGCCTCTTC CAAAAAGGCC 
AAGAGAGACC TCATAGATAA CTCCTTCAAC CGGTACACAT TTAATGAGGA TGAGGGGGAG 
CTTCCGGAGT GGTTTGTGCA AGAGGAAAAG CAGCACCGGA TACGACAGTT GCCTGTTGGT 
AAGAAGGAGG TGGAGCATTA CCGGAAACGC TGGCGGGAAA TCAATGCACG TCCCATCAAG 
AAGGTGGCTG AGGCTAAGGC TAGAAAGAAA AGGAGGATGC TGAAGAGGCT GGAGCAGACC 
AGGAAGAAGG CAGAAGCCGT GGTGAACACA GTGGACATCT CAGAACGAGA GAAAGTGGCA 
CAGCTGCGAA GTCTCTACAA GAAGGCTGGG CTTGGCAAGG AGAAACGCCA TGTCACCTAC 
GTTGTAGCCA AAAAAGGTGT GGGCCGCAAA GTGCGCCGGC CAGCTGGAGT CAGAGGTCAT 
TTCAAGGTGG TGGACTCAAG GATGAAGAAG GACCAAAGAG CACAGCAACG TAAGGAACAA 
AAGAAAAAAC ACAAACGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060117.3
CDS	647..3190
Translation	MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVGVDLVPIKPLPNVVTLQQDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGSFITKVFRSRDYQPLLWIFQQLFRRVQATKPQASRHESAEIFVVCQGFLAPDKVDSKFFDPKFAFKEVEVQAKTVTELVTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEIMVDDEELAQHPATTEDIRVCCQDIRVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSGEEDEGDEEDSTAGTTKQPSKEEEEEEEEEQLNQTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFSLSTIRGHQLLEEVTQGDMSAADTFLSDLPRDDIYVSDVEDDGDDTSLDSDLDPEELAGVRGHQGLRDQKRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK

Target ORF information:

RefSeq Version	NM_017647.3
Organism	Homo sapiens(human)
Definition	Homo sapiens FtsJ RNA methyltransferase homolog 3 (FTSJ3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017647.3

ORF Insert Sequence:

ATGGGCAAGA AGGGCAAAGT TGGCAAGAGC CGACGAGACA AGTTTTATCA CTTGGCGAAG 
GAGACGGGTT ACCGTTCCCG ATCTGCTTTC AAGCTGATCC AGCTCAATCG CCGCTTTCAG 
TTCCTGCAGA AAGCCCGAGC CTTGCTGGAC CTGTGTGCTG CGCCAGGGGG ATGGCTGCAG 
GTAGCTGCCA AGTTTATGCC TGTATCCAGC CTTATTGTGG GAGTGGACCT GGTTCCAATC 
AAGCCTCTCC CCAATGTGGT GACTCTCCAG CAGGACATCA CAACAGAACG TTGTAGGCAG 
GCCCTGAGGA AGGAGCTGAA GACCTGGAAG GTTGATGTTG TGCTCAATGA TGGGGCCCCC 
AACGTTGGGG CTAGCTGGGT CCATGATGCT TACTCACAAG CCCATTTGAC ACTGATGGCT 
CTACGTTTGG CTTGTGACTT TTTGGCCCGT GGTGGCAGCT TCATCACAAA GGTTTTCCGT 
TCTCGTGACT ATCAGCCTCT GCTATGGATC TTTCAGCAGC TGTTCCGCCG TGTCCAGGCC 
ACCAAGCCCC AAGCCTCTCG CCATGAATCT GCAGAGATCT TTGTAGTCTG CCAAGGATTC 
CTGGCCCCTG ACAAGGTTGA CAGTAAATTC TTTGACCCCA AATTTGCCTT TAAGGAGGTT 
GAAGTTCAGG CTAAGACCGT TACTGAATTG GTTACTAAGA AGAAGCCAAA GGCTGAAGGC 
TATGCTGAGG GTGACCTCAC TCTCTATCAC CGTACCTCAG TCACTGACTT CCTCCGAGCT 
GCCAACCCTG TTGACTTCCT CTCCAAGGCC AGCGAAATCA TGGTAGATGA TGAAGAGTTG 
GCACAGCATC CAGCTACCAC TGAGGACATA CGGGTGTGCT GTCAGGACAT CAGAGTGTTG 
GGGCGCAAGG AGCTCAGGTC GCTACTAAAC TGGAGAACAA AACTTCGGCG ATATGTGGCC 
AAGAAGCTGA AAGAACAAGC AAAGGCACTG GACATCAGCC TCAGCTCTGG AGAGGAAGAT 
GAAGGTGATG AGGAGGACTC AACAGCTGGA ACCACAAAGC AGCCCTCTAA GGAGGAGGAG 
GAAGAGGAGG AGGAGGAACA ACTGAACCAG ACCTTGGCAG AAATGAAGGC CCAGGAGGTG 
GCGGAATTGA AGAGGAAGAA AAAGAAGCTG TTGCGTGAGC AGAGAAAGCA GCGGGAGCGT 
GTGGAGCTGA AGATGGATCT GCCTGGGGTT TCCATTGCAG ACGAGGGGGA GACTGGCATG 
TTCTCCTTGA GCACCATCCG GGGTCACCAG TTATTAGAGG AAGTAACACA AGGGGATATG 
AGTGCAGCAG ACACATTTCT GTCCGATCTG CCAAGGGATG ATATCTATGT GTCAGATGTT 
GAGGACGACG GTGATGACAC ATCTCTGGAT AGTGACCTGG ATCCAGAGGA GCTGGCAGGA 
GTCAGGGGAC ATCAGGGTCT AAGGGACCAA AAGCGTATGC GACTTACTGA AGTGCAAGAT 
GATAAAGAGG AGGAGGAGGA GGAGAATCCA CTGCTGGTAC CACTGGAGGA AAAGGCAGTA 
CTGCAGGAAG AACAAGCCAA CCTGTGGTTC TCAAAGGGCA GCTTTGCTGG GATCGAGGAC 
GATGCCGATG AGGCCCTGGA GATCAGTCAG GCCCAGCTGT TATTTGAGAA CCGGCGGAAG 
GGACGGCAGC AGCAGCAGAA GCAGCAGCTG CCACAGACAC CCCCTTCCTG TTTGAAGACT 
GAGATAATGT CTCCCCTGTA CCAAGATGAA GCCCCTAAGG GAACAGAGGC TTCTTCGGGG 
ACAGAAGCTG CCACTGGCCT TGAAGGGGAA GAAAAGGATG GCATCTCAGA CAGTGATAGC 
AGTACTAGCA GTGAGGAAGA AGAGAGCTGG GAACCCCTCC GTGGTAAGAA GCGAAGCCGT 
GGGCCTAAGT CAGATGATGA CGGGTTTGAG ATAGTGCCTA TTGAGGACCC AGCGAAACAT 
CGGATACTGG ACCCCGAAGG CCTTGCTCTA GGTGCTGTTA TTGCCTCTTC CAAAAAGGCC 
AAGAGAGACC TCATAGATAA CTCCTTCAAC CGGTACACAT TTAATGAGGA TGAGGGGGAG 
CTTCCGGAGT GGTTTGTGCA AGAGGAAAAG CAGCACCGGA TACGACAGTT GCCTGTTGGT 
AAGAAGGAGG TGGAGCATTA CCGGAAACGC TGGCGGGAAA TCAATGCACG TCCCATCAAG 
AAGGTGGCTG AGGCTAAGGC TAGAAAGAAA AGGAGGATGC TGAAGAGGCT GGAGCAGACC 
AGGAAGAAGG CAGAAGCCGT GGTGAACACA GTGGACATCT CAGAACGAGA GAAAGTGGCA 
CAGCTGCGAA GTCTCTACAA GAAGGCTGGG CTTGGCAAGG AGAAACGCCA TGTCACCTAC 
GTTGTAGCCA AAAAAGGTGT GGGCCGCAAA GTGCGCCGGC CAGCTGGAGT CAGAGGTCAT 
TTCAAGGTGG TGGACTCAAG GATGAAGAAG GACCAAAGAG CACAGCAACG TAAGGAACAA 
AAGAAAAAAC ACAAACGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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FTSJ3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

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FAX

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