VTI1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VTI1B
Entrez Gene ID	10490
Full Name	vesicle transport through interaction with t-SNAREs 1B
Synonyms	VTI1,VTI1-LIKE,VTI1L,VTI2,v-SNARE,vti1-rp1
General protein information	Preferred Names vesicle transport through interaction with t-SNAREs 1B Names vesicle transport through interaction with t-SNAREs homolog 1B vesicle transport v-SNARE protein Vti1-like 1 vesicle-associated soluble NSF attachment protein receptor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.1
Disorder MIM:	603207

mRNA and Protein(s)

mRNA	Protein	Name
NM_006370.2	NP_006361.1	vesicle transport through interaction with t-SNAREs homolog 1B
NM_006370.3	NP_006361.1	vesicle transport through interaction with t-SNAREs homolog 1B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	VTI1B	XP_421192.1
	VTI1B	XP_001107598.1
Canis lupus familiaris (dog)	VTI1B	XP_537489.2
Bos taurus (cattle)	VTI1B	NP_001073115.1
Mus musculus (house mouse)	Vti1b	NP_058080.2
Homo sapiens (human)	VTI1B	NP_006361.1
Rattus norvegicus (Norway rat)	Vti1b	XP_003750244.1
Xenopus tropicalis (tropical clawed frog)	vti1b	NP_001016960.1
Pan troglodytes (chimpanzee)	VTI1B	NP_001238940.1
Danio rerio (zebrafish)	vti1b	NP_957330.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.
Nozawa T, Minowa-Nozawa A, Aikawa C, Nakagawa I
Autophagy13(1)57-69(2017 Jan)

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Pirooz SD, He S, Zhang T, Zhang X, Zhao Z, Oh S, O'Connell D, Khalilzadeh P, Amini-Bavil-Olyaee S, Farzan M, Liang C
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)

The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Itakura E, Kishi-Itakura C, Mizushima N
Cell151(6)1256-69(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RABGEF1 mediates recycling endosome fusion with GAS-containing autophagosome-like vacuoles through the STX6-VAMP3-VTI1B complex; SNAREs are involved in autophagosome formation in response to bacterial infection
Title: The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.

Vti1b-dependent tethering of Lytic granule and CD3-endo determines accumulation, docking, and efficient lytic granule secretion at the immunological synapse.
Title: Docking of lytic granules at the immunological synapse in human CTL requires Vti1b-dependent pairing with CD3 endosomes.

Results suggest that the combinational SNARE proteins VAMP8 and Vti1b mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation.
Title: Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes.

Results report that syntaxin 7, syntaxin 8, vti1b and VAMP8 physically and functionally interact with CFTR.
Title: Endosomal SNARE proteins regulate CFTR activity and trafficking in epithelial cells.

epsin 4 epsin-related protein is an adaptor for vti1b
Title: EpsinR is an adaptor for the SNARE protein Vti1b.

The following VTI1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VTI1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05533	NM_006370.3 Latest version!	Homo sapiens vesicle transport through interaction with t-SNAREs 1B (VTI1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu05533	NM_006370.2	Homo sapiens vesicle transport through interaction with t-SNAREs 1B (VTI1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VTI1B ( NM_006370.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006361.1 Homo sapiens vesicle transport through interaction with t-SNAREs 1B (VTI1B), mRNA.

Price & Availability↑

CloneID	OHu05533
Clone ID Related Accession (Same CDS sequence)	NM_006370.2 , NM_006370.3
Accession Version	NM_006370.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	vesicle transport through interaction with t-SNAREs homolog 1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI597967.1 and BC003142.1. On Jan 28, 2009 this sequence version replaced NM_006370.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.482040.1, SRR1803611.204896.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTCCT CCGCCGCCTC CTCGGAGCAT TTCGAGAAGC TGCACGAGAT CTTCCGCGGC 
CTCCATGAAG ACCTACAAGG GGTGCCCGAG CGGCTGCTGG GGACGGCGGG GACCGAAGAA 
AAGAAGAAAT TGATCAGGGA TTTTGATGAA AAGCAACAGG AAGCAAATGA AACGCTGGCA 
GAGATGGAGG AGGAGCTACG TTATGCACCC CTGTCTTTCC GAAACCCCAT GATGTCTAAG 
CTTCGAAACT ACCGGAAGGA CCTTGCTAAA CTCCATCGGG AGGTGAGAAG CACACCTTTG 
ACAGCCACAC CTGGAGGCCG AGGAGACATG AAATATGGCA TATATGCTGT AGAGAATGAG 
CATATGAATC GGCTACAGTC TCAAAGGGCA ATGCTTCTGC AGGGCACTGA AAGCCTGAAC 
CGGGCCACCC AAAGTATTGA ACGTTCTCAT CGGATTGCCA CAGAGACTGA CCAGATTGGC 
TCAGAAATCA TAGAAGAGCT GGGGGAACAA CGAGACCAGT TAGAACGTAC CAAGAGTAGA 
CTGGTAAACA CAAGTGAAAA CTTGAGCAAA AGTCGGAAGA TTCTCCGTTC AATGTCCAGA 
AAAGTGACAA CCAACAAGCT GCTGCTTTCC ATTATCATCT TACTGGAGCT CGCCATCCTG 
GGAGGCCTGG TTTACTACAA ATTCTTTCGC AGCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006361.1
CDS	397..1095
Translation	MASSAASSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH

Target ORF information:

RefSeq Version	NM_006370.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle transport through interaction with t-SNAREs 1B (VTI1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006370.2

ORF Insert Sequence:

ATGGCCTCCT CCGCCGCCTC CTCGGAGCAT TTCGAGAAGC TGCACGAGAT CTTCCGCGGC 
CTCCATGAAG ACCTACAAGG GGTGCCCGAG CGGCTGCTGG GGACGGCGGG GACCGAAGAA 
AAGAAGAAAT TGATCAGGGA TTTTGATGAA AAGCAACAGG AAGCAAATGA AACGCTGGCA 
GAGATGGAGG AGGAGCTACG TTATGCACCC CTGTCTTTCC GAAACCCCAT GATGTCTAAG 
CTTCGAAACT ACCGGAAGGA CCTTGCTAAA CTCCATCGGG AGGTGAGAAG CACACCTTTG 
ACAGCCACAC CTGGAGGCCG AGGAGACATG AAATATGGCA TATATGCTGT AGAGAATGAG 
CATATGAATC GGCTACAGTC TCAAAGGGCA ATGCTTCTGC AGGGCACTGA AAGCCTGAAC 
CGGGCCACCC AAAGTATTGA ACGTTCTCAT CGGATTGCCA CAGAGACTGA CCAGATTGGC 
TCAGAAATCA TAGAAGAGCT GGGGGAACAA CGAGACCAGT TAGAACGTAC CAAGAGTAGA 
CTGGTAAACA CAAGTGAAAA CTTGAGCAAA AGTCGGAAGA TTCTCCGTTC AATGTCCAGA 
AAAGTGACAA CCAACAAGCT GCTGCTTTCC ATTATCATCT TACTGGAGCT CGCCATCCTG 
GGAGGCCTGG TTTACTACAA ATTCTTTCGC AGCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VTI1B ( NM_006370.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006361.1 Homo sapiens vesicle transport through interaction with t-SNAREs 1B (VTI1B), mRNA.

Price & Availability↑

CloneID	OHu05533
Clone ID Related Accession (Same CDS sequence)	NM_006370.2 , NM_006370.3
Accession Version	NM_006370.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	vesicle transport through interaction with t-SNAREs homolog 1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC003142.1 and AL049779.6. On Nov 23, 2018 this sequence version replaced NM_006370.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.144867.1, SRR1803617.30613.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000554659.6/ ENSP00000450731.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCCT CCGCCGCCTC CTCGGAGCAT TTCGAGAAGC TGCACGAGAT CTTCCGCGGC 
CTCCATGAAG ACCTACAAGG GGTGCCCGAG CGGCTGCTGG GGACGGCGGG GACCGAAGAA 
AAGAAGAAAT TGATCAGGGA TTTTGATGAA AAGCAACAGG AAGCAAATGA AACGCTGGCA 
GAGATGGAGG AGGAGCTACG TTATGCACCC CTGTCTTTCC GAAACCCCAT GATGTCTAAG 
CTTCGAAACT ACCGGAAGGA CCTTGCTAAA CTCCATCGGG AGGTGAGAAG CACACCTTTG 
ACAGCCACAC CTGGAGGCCG AGGAGACATG AAATATGGCA TATATGCTGT AGAGAATGAG 
CATATGAATC GGCTACAGTC TCAAAGGGCA ATGCTTCTGC AGGGCACTGA AAGCCTGAAC 
CGGGCCACCC AAAGTATTGA ACGTTCTCAT CGGATTGCCA CAGAGACTGA CCAGATTGGC 
TCAGAAATCA TAGAAGAGCT GGGGGAACAA CGAGACCAGT TAGAACGTAC CAAGAGTAGA 
CTGGTAAACA CAAGTGAAAA CTTGAGCAAA AGTCGGAAGA TTCTCCGTTC AATGTCCAGA 
AAAGTGACAA CCAACAAGCT GCTGCTTTCC ATTATCATCT TACTGGAGCT CGCCATCCTG 
GGAGGCCTGG TTTACTACAA ATTCTTTCGC AGCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006361.1
CDS	144..842
Translation	MASSAASSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH

Target ORF information:

RefSeq Version	NM_006370.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle transport through interaction with t-SNAREs 1B (VTI1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006370.3

ORF Insert Sequence:

ATGGCCTCCT CCGCCGCCTC CTCGGAGCAT TTCGAGAAGC TGCACGAGAT CTTCCGCGGC 
CTCCATGAAG ACCTACAAGG GGTGCCCGAG CGGCTGCTGG GGACGGCGGG GACCGAAGAA 
AAGAAGAAAT TGATCAGGGA TTTTGATGAA AAGCAACAGG AAGCAAATGA AACGCTGGCA 
GAGATGGAGG AGGAGCTACG TTATGCACCC CTGTCTTTCC GAAACCCCAT GATGTCTAAG 
CTTCGAAACT ACCGGAAGGA CCTTGCTAAA CTCCATCGGG AGGTGAGAAG CACACCTTTG 
ACAGCCACAC CTGGAGGCCG AGGAGACATG AAATATGGCA TATATGCTGT AGAGAATGAG 
CATATGAATC GGCTACAGTC TCAAAGGGCA ATGCTTCTGC AGGGCACTGA AAGCCTGAAC 
CGGGCCACCC AAAGTATTGA ACGTTCTCAT CGGATTGCCA CAGAGACTGA CCAGATTGGC 
TCAGAAATCA TAGAAGAGCT GGGGGAACAA CGAGACCAGT TAGAACGTAC CAAGAGTAGA 
CTGGTAAACA CAAGTGAAAA CTTGAGCAAA AGTCGGAAGA TTCTCCGTTC AATGTCCAGA 
AAAGTGACAA CCAACAAGCT GCTGCTTTCC ATTATCATCT TACTGGAGCT CGCCATCCTG 
GGAGGCCTGG TTTACTACAA ATTCTTTCGC AGCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes.
Autophagy13(1)57-69(2017 Jan)
Nozawa T,Minowa-Nozawa A,Aikawa C,Nakagawa I

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.
Proceedings of the National Academy of Sciences of the United States of America111(7)2716-21(2014 Feb)
Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
Cell151(6)1256-69(2012 Dec)
Itakura E,Kishi-Itakura C,Mizushima N