Ano2 cDNA ORF clone, Heterocephalus glaber(naked mole-rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ano2
Entrez Gene ID	101697854
Full Name	anoctamin 2
Gene Type	protein-coding
Organism	Heterocephalus glaber(naked mole-rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004869378.2	XP_004869435.1	anoctamin-2
XM_021248110.1	XP_021103769.1	anoctamin-2

Pathways from BioSystems
Gene Ontology

The following Ano2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ano2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHe222324	XM_021248110.1 Latest version!	Heterocephalus glaber anoctamin 2 (Ano2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OHe60681	XM_004869378.2 Latest version!	Heterocephalus glaber anoctamin 2, calcium activated chloride channel (Ano2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ano2 ( XM_021248110.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021103769.1 Heterocephalus glaber anoctamin 2 (Ano2), mRNA.

Price & Availability↑

CloneID	OHe222324
Clone ID Related Accession (Same CDS sequence)	XM_021248110.1
Accession Version	XM_021248110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	anoctamin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624860.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTCC ACGACACCCA GAGGAAGGTC GACTACGTGC TCGCCTACCA CTACCGGAAA 
CGTGGGGTGC ACCATGGCTC CCCTGCCCAC TCACTGGCTG TAATCTCCAA TGGGGAGACG 
GGCAAGGACC GCCATGCTGG GGGACCCGGT GACATCGAGC TGGGGCCACT TGATGCCCTG 
GAGGAGGAGC GGAGGGAACA GCGGGAAGAG TTTGAGAGCA ACCTGATGGC AGCCGGCCTG 
GAGCTGGAGA AGGACTTGGA GAGCAAAAGC CAGGGATCCG TCTTTGTCCG GATACACGCC 
CCGTGGCAGG TGCTGGCCAG AGAGGCAGAA TTCTTGAAGA TCAAAGTCCC CACCAAGAAA 
ATGTACGAGA TCAAAGCAGG AGGCAGCATC ACGAAGAAGC TCAACACGGT TCTGCAGAAG 
CTGAGTGTGC CGCTGCAGCC CAGAGTTCCG GAGCACAGCA ACAACAGGAT GAAGAGCCTC 
TCCTACCCGT TCTCCCGGGA GAAAATGTAC CTGTACAACA TCCAGGAAAA GGACACCTTC 
TTTGACAGTG CCACCCGTAG CCGCATTGTG CACGAGATCC TGAAGCGCAC GGCCTGTTCC 
CGCGCCAACA ACACGATGGG TATTAACTCT CTGATAGCAA ACAACATCTA TGAGGCTGCC 
TACCCGCTTC ATGACGGTGA ATATGACAGT GCAGGGGAGG ACATGAATGA CAGGAAGCTG 
CTGTATCAAG AGTGGGCACG CTATGGAGTG TTTTATAAGT TTCAACCCAT TGACCTCATC 
AGAAAGTATT TTGGAGAAAA AATTGGACTG TATTTTGCCT GGCTGGGATT ATATACATCA 
TTCCTCATCC CGTCTTCTAT AATTGGGGTC ATCGTGTTTC TGTATGGATG TGCAACAATC 
GAGGAAGATA TTCCCAGCAA AGAGATGTGT GACCAGCAGA ATGCCTTCAC CATGTGTCCC 
CTTTGTGACA AGTCCTGTGA CTACTGGAAC CTCAGCTCAG CCTGCAGGAC GGCACGGGCC 
AGCCACCTGT TTGACAATCC TGCTACTGTC TTCTTTTCCA TCTTCATGGC TCTGTGGGCC 
ACCATGTTTC TGGAGAACTG GAAAAGACTA CAGATGCGAC TGGGCTACTT CTGGGACTTG 
ACTGGTATAG AAGAGGAAGA AGAGCACTCC CGGCCTGAGT ATGAAACCAG AGTTCGGGAG 
AAAATGTTAA AGGAGAACGA CAAGTCGGTG GTCCAGAAAT TGGAAACAAA TGCGACTGAA 
GGAGGAGATG ACGGGGGCGA TGATGAAGAC AAACTGACCT GGAAGGATCG GTTCCCGGGT 
TACTTGATGA GCTTTGCCTC CATCTTGTTC ATGATTGCCT TGACGTTCTC GATCGTCTTT 
GGGGTCATTG TATATCGGAT CACAACTGCA GCTGCTCTGT CTCTCAACAA GGCCACGCGC 
TCCAATGTCC GGGTGACAGT CACGGCAACA GCAGTCATCA TCAACCTTGT AGTCATCCTC 
ATTCTGGATG AGATCTATGG GGCTGTGGCC AAGTGGCTCA CCAAAATAGA GGTTCCAAAA 
ACAGAACAGA CTTTTGAAGA GCGCTTGATA CTCAAAGCTT TCTTGCTAAA GTTTGTCAAC 
GCCTACTCTC CCATCTTCTA CGTGGCCTTT TTCAAGGGCA GGTTTGTGGG CAGGCCTGGA 
AGCTATGTCT ATGTGTTTGA TGGCTACCGC ATGGAAGAGT GTGCTCCAGG GGGCTGCCTC 
ATGGAGCTCT GCATTCAGCT CAGCATCATC ATGTTGGGAA AGCAGTTGAT CCAGAACAAC 
ATCTTTGAGA TTGGAGTTCC GAAACTAAAG AAACTATTTC GGAAGCTGAA AGATGAGACA 
GAACCTGGAG AAGCTGACCC TGAGCATTCA AAACCTCCAG AGCAGTGGGA CTTAGACTAC 
AGCCTGGAGC CGTACACAGG GCTGACTCCG GAGTACATGG AAATGATCAT CCAGTTCGGT 
TTTGTCACTC TCTTTGTGGC CTCCTTCCCT CTGGCACCCG TGTTTGCCCT CCTCAACAAT 
GTCATTGAAG TGCGGCTAGA TGCAAAAAAG TTCGTCACGG AGCTGAGACG GCCGGATGCT 
GTGAGAACCA AAGATATTGG AATTTGGTTT GACATTCTTT CCGGAATTGG CAAGTTCTCT 
GTTATCATCA ACGCATTCGT CATCGCCGTC ACCTCCGACT TCATCCCCCG CCTGGTGTAC 
CAGTACTCCT ACAGTCACAA TGGGACTCTG CACGGCTTTG TGAACCACAC GCTGTCCTTC 
TTCAACGTCA GCCAGCTGAA GGAGGGGACA CAGCCAGAGA ACTCACAGTT CGACCAGGAG 
GTTCAGTTCT GCAGGTTCAA GGATTACCGA GAGCCGCCAT GGGCACCGAA CCCATATGAG 
TTTTCCAAAC AGTACTGGTC TGTTCTGTCT GCTCGACTGG CTTTCGTCAT AATCTTCCAG 
AACCTGGTGA TGTTCCTGAG CGTCCTTGTG GACTGGATGA TTCCAGACAT CCCCACGGAC 
ATCAGCGACC AGATCAAGAA AGAGAAGAGT TTGTTGGTGG ACTTCTTCCT GAAAGAGGAG 
CACGAGAAGC TGAGGCTCAC TGATGAGCCA GCTCAGAGGA GCCAGGGAGG TGGGGACAGC 
AGCAGGAGGA GCCAGAGTGG CGCAGCCAGC TCAGCTCCCT CGGCCCTGAG CCCCCCTGGC 
AGCAGAGCGT CTTCCGGCTC CCAGCACACC AACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021103769.1
CDS	241..2979
Translation	MHFHDTQRKVDYVLAYHYRKRGVHHGSPAHSLAVISNGETGKDRHAGGPGDIELGPLDALEEERREQREEFESNLMAAGLELEKDLESKSQGSVFVRIHAPWQVLAREAEFLKIKVPTKKMYEIKAGGSITKKLNTVLQKLSVPLQPRVPEHSNNRMKSLSYPFSREKMYLYNIQEKDTFFDSATRSRIVHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSAGEDMNDRKLLYQEWARYGVFYKFQPIDLIRKYFGEKIGLYFAWLGLYTSFLIPSSIIGVIVFLYGCATIEEDIPSKEMCDQQNAFTMCPLCDKSCDYWNLSSACRTARASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEEHSRPEYETRVREKMLKENDKSVVQKLETNATEGGDDGGDDEDKLTWKDRFPGYLMSFASILFMIALTFSIVFGVIVYRITTAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLKKLFRKLKDETEPGEADPEHSKPPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNNVIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVIINAFVIAVTSDFIPRLVYQYSYSHNGTLHGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWSVLSARLAFVIIFQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLRLTDEPAQRSQGGGDSSRRSQSGAASSAPSALSPPGSRASSGSQHTNL

Target ORF information:

RefSeq Version	XM_021248110.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber anoctamin 2 (Ano2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021248110.1

ORF Insert Sequence:

ATGCATTTCC ACGACACCCA GAGGAAGGTC GACTACGTGC TCGCCTACCA CTACCGGAAA 
CGTGGGGTGC ACCATGGCTC CCCTGCCCAC TCACTGGCTG TAATCTCCAA TGGGGAGACG 
GGCAAGGACC GCCATGCTGG GGGACCCGGT GACATCGAGC TGGGGCCACT TGATGCCCTG 
GAGGAGGAGC GGAGGGAACA GCGGGAAGAG TTTGAGAGCA ACCTGATGGC AGCCGGCCTG 
GAGCTGGAGA AGGACTTGGA GAGCAAAAGC CAGGGATCCG TCTTTGTCCG GATACACGCC 
CCGTGGCAGG TGCTGGCCAG AGAGGCAGAA TTCTTGAAGA TCAAAGTCCC CACCAAGAAA 
ATGTACGAGA TCAAAGCAGG AGGCAGCATC ACGAAGAAGC TCAACACGGT TCTGCAGAAG 
CTGAGTGTGC CGCTGCAGCC CAGAGTTCCG GAGCACAGCA ACAACAGGAT GAAGAGCCTC 
TCCTACCCGT TCTCCCGGGA GAAAATGTAC CTGTACAACA TCCAGGAAAA GGACACCTTC 
TTTGACAGTG CCACCCGTAG CCGCATTGTG CACGAGATCC TGAAGCGCAC GGCCTGTTCC 
CGCGCCAACA ACACGATGGG TATTAACTCT CTGATAGCAA ACAACATCTA TGAGGCTGCC 
TACCCGCTTC ATGACGGTGA ATATGACAGT GCAGGGGAGG ACATGAATGA CAGGAAGCTG 
CTGTATCAAG AGTGGGCACG CTATGGAGTG TTTTATAAGT TTCAACCCAT TGACCTCATC 
AGAAAGTATT TTGGAGAAAA AATTGGACTG TATTTTGCCT GGCTGGGATT ATATACATCA 
TTCCTCATCC CGTCTTCTAT AATTGGGGTC ATCGTGTTTC TGTATGGATG TGCAACAATC 
GAGGAAGATA TTCCCAGCAA AGAGATGTGT GACCAGCAGA ATGCCTTCAC CATGTGTCCC 
CTTTGTGACA AGTCCTGTGA CTACTGGAAC CTCAGCTCAG CCTGCAGGAC GGCACGGGCC 
AGCCACCTGT TTGACAATCC TGCTACTGTC TTCTTTTCCA TCTTCATGGC TCTGTGGGCC 
ACCATGTTTC TGGAGAACTG GAAAAGACTA CAGATGCGAC TGGGCTACTT CTGGGACTTG 
ACTGGTATAG AAGAGGAAGA AGAGCACTCC CGGCCTGAGT ATGAAACCAG AGTTCGGGAG 
AAAATGTTAA AGGAGAACGA CAAGTCGGTG GTCCAGAAAT TGGAAACAAA TGCGACTGAA 
GGAGGAGATG ACGGGGGCGA TGATGAAGAC AAACTGACCT GGAAGGATCG GTTCCCGGGT 
TACTTGATGA GCTTTGCCTC CATCTTGTTC ATGATTGCCT TGACGTTCTC GATCGTCTTT 
GGGGTCATTG TATATCGGAT CACAACTGCA GCTGCTCTGT CTCTCAACAA GGCCACGCGC 
TCCAATGTCC GGGTGACAGT CACGGCAACA GCAGTCATCA TCAACCTTGT AGTCATCCTC 
ATTCTGGATG AGATCTATGG GGCTGTGGCC AAGTGGCTCA CCAAAATAGA GGTTCCAAAA 
ACAGAACAGA CTTTTGAAGA GCGCTTGATA CTCAAAGCTT TCTTGCTAAA GTTTGTCAAC 
GCCTACTCTC CCATCTTCTA CGTGGCCTTT TTCAAGGGCA GGTTTGTGGG CAGGCCTGGA 
AGCTATGTCT ATGTGTTTGA TGGCTACCGC ATGGAAGAGT GTGCTCCAGG GGGCTGCCTC 
ATGGAGCTCT GCATTCAGCT CAGCATCATC ATGTTGGGAA AGCAGTTGAT CCAGAACAAC 
ATCTTTGAGA TTGGAGTTCC GAAACTAAAG AAACTATTTC GGAAGCTGAA AGATGAGACA 
GAACCTGGAG AAGCTGACCC TGAGCATTCA AAACCTCCAG AGCAGTGGGA CTTAGACTAC 
AGCCTGGAGC CGTACACAGG GCTGACTCCG GAGTACATGG AAATGATCAT CCAGTTCGGT 
TTTGTCACTC TCTTTGTGGC CTCCTTCCCT CTGGCACCCG TGTTTGCCCT CCTCAACAAT 
GTCATTGAAG TGCGGCTAGA TGCAAAAAAG TTCGTCACGG AGCTGAGACG GCCGGATGCT 
GTGAGAACCA AAGATATTGG AATTTGGTTT GACATTCTTT CCGGAATTGG CAAGTTCTCT 
GTTATCATCA ACGCATTCGT CATCGCCGTC ACCTCCGACT TCATCCCCCG CCTGGTGTAC 
CAGTACTCCT ACAGTCACAA TGGGACTCTG CACGGCTTTG TGAACCACAC GCTGTCCTTC 
TTCAACGTCA GCCAGCTGAA GGAGGGGACA CAGCCAGAGA ACTCACAGTT CGACCAGGAG 
GTTCAGTTCT GCAGGTTCAA GGATTACCGA GAGCCGCCAT GGGCACCGAA CCCATATGAG 
TTTTCCAAAC AGTACTGGTC TGTTCTGTCT GCTCGACTGG CTTTCGTCAT AATCTTCCAG 
AACCTGGTGA TGTTCCTGAG CGTCCTTGTG GACTGGATGA TTCCAGACAT CCCCACGGAC 
ATCAGCGACC AGATCAAGAA AGAGAAGAGT TTGTTGGTGG ACTTCTTCCT GAAAGAGGAG 
CACGAGAAGC TGAGGCTCAC TGATGAGCCA GCTCAGAGGA GCCAGGGAGG TGGGGACAGC 
AGCAGGAGGA GCCAGAGTGG CGCAGCCAGC TCAGCTCCCT CGGCCCTGAG CCCCCCTGGC 
AGCAGAGCGT CTTCCGGCTC CCAGCACACC AACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ano2 ( XM_004869378.2 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_004869435.1 Heterocephalus glaber anoctamin 2, calcium activated chloride channel (Ano2), mRNA.

Price & Availability↑

CloneID	OHe60681
Clone ID Related Accession (Same CDS sequence)	XM_004869378.2
Accession Version	XM_004869378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-01
Organism	Heterocephalus glaber(Naked mole-rat)
Product	anoctamin-2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624860.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 2, 2015 this sequence version replaced XM_004869378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTCC ACGACACCCA GAGGAAGGTC GACTACGTGC TCGCCTACCA CTACCGGAAA 
CGTGGGGTGC ACCATGGCTC CCCTGCCCAC TCACTGGCTG TAATCTCCAA TGGGGAGACG 
GGCAAGGACC GCCATGCTGG GGGACCCGGT GACATCGAGC TGGGGCCACT TGATGCCCTG 
GAGGAGGAGC GGAGGGAACA GCGGGAAGAG TTTGAGAGCA ACCTGATGGC AGCCGGCCTG 
GAGCTGGAGA AGGACTTGGA GAGCAAAAGC CAGGGATCCG TCTTTGTCCG GATACACGCC 
CCGTGGCAGG TGCTGGCCAG AGAGGCAGAA TTCTTGAAGA TCAAAGTCCC CACCAAGAAA 
ATGTACGAGA TCAAAGCAGG AGGCAGCATC ACGAAGAAGC TCAACACGGT TCTGCAGAAG 
CTGAGTGTGC CGCTGCAGCC CAGAGTTCCG GAGCACAGCA ACAACAGGAT GAAGAGCCTC 
TCCTACCCGT TCTCCCGGGA GAAAATGTAC CTGTACAACA TCCAGGAAAA GGACACCTTC 
TTTGACAGTG CCACCCGTAG CCGCATTGTG CACGAGATCC TGAAGCGCAC GGCCTGTTCC 
CGCGCCAACA ACACGATGGG TATTAACTCT CTGATAGCAA ACAACATCTA TGAGGCTGCC 
TACCCGCTTC ATGACGGTGA ATATGACAGT GCAGGGGAGG ACATGAATGA CAGGAAGCTG 
CTGTATCAAG AGTGGGCACG CTATGGAGTG TTTTATAAGT TTCAACCCAT TGACCTCATC 
AGAAAGTATT TTGGAGAAAA AATTGGACTG TATTTTGCCT GGCTGGGATT ATATACATCA 
TTCCTCATCC CGTCTTCTAT AATTGGGGTC ATCGTGTTTC TGTATGGATG TGCAACAATC 
GAGGAAGATA TTCCCAGCAA AGAGATGTGT GACCAGCAGA ATGCCTTCAC CATGTGTCCC 
CTTTGTGACA AGTCCTGTGA CTACTGGAAC CTCAGCTCAG CCTGCAGGAC GGCACGGGCC 
AGCCACCTGT TTGACAATCC TGCTACTGTC TTCTTTTCCA TCTTCATGGC TCTGTGGGCC 
ACCATGTTTC TGGAGAACTG GAAAAGACTA CAGATGCGAC TGGGCTACTT CTGGGACTTG 
ACTGGTATAG AAGAGGAAGA AGAGCGAGCC CAGGAGCACT CCCGGCCTGA GTATGAAACC 
AGAGTTCGGG AGAAAATGTT AAAGGAGAAC GACAAGTCGG TGGTCCAGAA ATTGGAAACA 
AATGCGACTG AAGGAGGAGA TGACGGGGGC GATGATGAAG ACAAACTGAC CTGGAAGGAT 
CGGTTCCCGG GTTACTTGAT GAGCTTTGCC TCCATCTTGT TCATGATTGC CTTGACGTTC 
TCGATCGTCT TTGGGGTCAT TGTATATCGG ATCACAACTG CAGCTGCTCT GTCTCTCAAC 
AAGGCCACGC GCTCCAATGT CCGGGTGACA GTCACGGCAA CAGCAGTCAT CATCAACCTT 
GTAGTCATCC TCATTCTGGA TGAGATCTAT GGGGCTGTGG CCAAGTGGCT CACCAAAATA 
GAGGTTCCAA AAACAGAACA GACTTTTGAA GAGCGCTTGA TACTCAAAGC TTTCTTGCTA 
AAGTTTGTCA ACGCCTACTC TCCCATCTTC TACGTGGCCT TTTTCAAGGG CAGGTTTGTG 
GGCAGGCCTG GAAGCTATGT CTATGTGTTT GATGGCTACC GCATGGAAGA GTGTGCTCCA 
GGGGGCTGCC TCATGGAGCT CTGCATTCAG CTCAGCATCA TCATGTTGGG AAAGCAGTTG 
ATCCAGAACA ACATCTTTGA GATTGGAGTT CCGAAACTAA AGAAACTATT TCGGAAGCTG 
AAAGATGAGA CAGAACCTGG AGAAGCTGAC CCTGAGCATT CAAAACCTCC AGAGCAGTGG 
GACTTAGACT ACAGCCTGGA GCCGTACACA GGGCTGACTC CGGAGTACAT GGAAATGATC 
ATCCAGTTCG GTTTTGTCAC TCTCTTTGTG GCCTCCTTCC CTCTGGCACC CGTGTTTGCC 
CTCCTCAACA ATGTCATTGA AGTGCGGCTA GATGCAAAAA AGTTCGTCAC GGAGCTGAGA 
CGGCCGGATG CTGTGAGAAC CAAAGATATT GGAATTTGGT TTGACATTCT TTCCGGAATT 
GGCAAGTTCT CTGTTATCAT CAACGCATTC GTCATCGCCG TCACCTCCGA CTTCATCCCC 
CGCCTGGTGT ACCAGTACTC CTACAGTCAC AATGGGACTC TGCACGGCTT TGTGAACCAC 
ACGCTGTCCT TCTTCAACGT CAGCCAGCTG AAGGAGGGGA CACAGCCAGA GAACTCACAG 
TTCGACCAGG AGGTTCAGTT CTGCAGGTTC AAGGATTACC GAGAGCCGCC ATGGGCACCG 
AACCCATATG AGTTTTCCAA ACAGTACTGG TCTGTTCTGT CTGCTCGACT GGCTTTCGTC 
ATAATCTTCC AGAACCTGGT GATGTTCCTG AGCGTCCTTG TGGACTGGAT GATTCCAGAC 
ATCCCCACGG ACATCAGCGA CCAGATCAAG AAAGAGAAGA GTTTGTTGGT GGACTTCTTC 
CTGAAAGAGG AGCACGAGAA GCTGAGGCTC ACTGATGAGC CAGCTCAGAG GAGCCAGGGA 
GGTGGGGACA GCAGCAGGAG GAGCCAGAGT GGCGCAGCCA GCTCAGCTCC CTCGGCCCTG 
AGCCCCCCTG GCAGCAGAGC GTCTTCCGGC TCCCAGCACA CCAACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004869435.1
CDS	146..2896
Translation	MHFHDTQRKVDYVLAYHYRKRGVHHGSPAHSLAVISNGETGKDRHAGGPGDIELGPLDALEEERREQREEFESNLMAAGLELEKDLESKSQGSVFVRIHAPWQVLAREAEFLKIKVPTKKMYEIKAGGSITKKLNTVLQKLSVPLQPRVPEHSNNRMKSLSYPFSREKMYLYNIQEKDTFFDSATRSRIVHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSAGEDMNDRKLLYQEWARYGVFYKFQPIDLIRKYFGEKIGLYFAWLGLYTSFLIPSSIIGVIVFLYGCATIEEDIPSKEMCDQQNAFTMCPLCDKSCDYWNLSSACRTARASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQEHSRPEYETRVREKMLKENDKSVVQKLETNATEGGDDGGDDEDKLTWKDRFPGYLMSFASILFMIALTFSIVFGVIVYRITTAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLKKLFRKLKDETEPGEADPEHSKPPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNNVIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVIINAFVIAVTSDFIPRLVYQYSYSHNGTLHGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWSVLSARLAFVIIFQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLRLTDEPAQRSQGGGDSSRRSQSGAASSAPSALSPPGSRASSGSQHTNL

Target ORF information:

RefSeq Version	XM_004869378.2
Organism	Heterocephalus glaber(Naked mole-rat)
Definition	Heterocephalus glaber anoctamin 2, calcium activated chloride channel (Ano2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004869378.2

ORF Insert Sequence:

ATGCATTTCC ACGACACCCA GAGGAAGGTC GACTACGTGC TCGCCTACCA CTACCGGAAA 
CGTGGGGTGC ACCATGGCTC CCCTGCCCAC TCACTGGCTG TAATCTCCAA TGGGGAGACG 
GGCAAGGACC GCCATGCTGG GGGACCCGGT GACATCGAGC TGGGGCCACT TGATGCCCTG 
GAGGAGGAGC GGAGGGAACA GCGGGAAGAG TTTGAGAGCA ACCTGATGGC AGCCGGCCTG 
GAGCTGGAGA AGGACTTGGA GAGCAAAAGC CAGGGATCCG TCTTTGTCCG GATACACGCC 
CCGTGGCAGG TGCTGGCCAG AGAGGCAGAA TTCTTGAAGA TCAAAGTCCC CACCAAGAAA 
ATGTACGAGA TCAAAGCAGG AGGCAGCATC ACGAAGAAGC TCAACACGGT TCTGCAGAAG 
CTGAGTGTGC CGCTGCAGCC CAGAGTTCCG GAGCACAGCA ACAACAGGAT GAAGAGCCTC 
TCCTACCCGT TCTCCCGGGA GAAAATGTAC CTGTACAACA TCCAGGAAAA GGACACCTTC 
TTTGACAGTG CCACCCGTAG CCGCATTGTG CACGAGATCC TGAAGCGCAC GGCCTGTTCC 
CGCGCCAACA ACACGATGGG TATTAACTCT CTGATAGCAA ACAACATCTA TGAGGCTGCC 
TACCCGCTTC ATGACGGTGA ATATGACAGT GCAGGGGAGG ACATGAATGA CAGGAAGCTG 
CTGTATCAAG AGTGGGCACG CTATGGAGTG TTTTATAAGT TTCAACCCAT TGACCTCATC 
AGAAAGTATT TTGGAGAAAA AATTGGACTG TATTTTGCCT GGCTGGGATT ATATACATCA 
TTCCTCATCC CGTCTTCTAT AATTGGGGTC ATCGTGTTTC TGTATGGATG TGCAACAATC 
GAGGAAGATA TTCCCAGCAA AGAGATGTGT GACCAGCAGA ATGCCTTCAC CATGTGTCCC 
CTTTGTGACA AGTCCTGTGA CTACTGGAAC CTCAGCTCAG CCTGCAGGAC GGCACGGGCC 
AGCCACCTGT TTGACAATCC TGCTACTGTC TTCTTTTCCA TCTTCATGGC TCTGTGGGCC 
ACCATGTTTC TGGAGAACTG GAAAAGACTA CAGATGCGAC TGGGCTACTT CTGGGACTTG 
ACTGGTATAG AAGAGGAAGA AGAGCGAGCC CAGGAGCACT CCCGGCCTGA GTATGAAACC 
AGAGTTCGGG AGAAAATGTT AAAGGAGAAC GACAAGTCGG TGGTCCAGAA ATTGGAAACA 
AATGCGACTG AAGGAGGAGA TGACGGGGGC GATGATGAAG ACAAACTGAC CTGGAAGGAT 
CGGTTCCCGG GTTACTTGAT GAGCTTTGCC TCCATCTTGT TCATGATTGC CTTGACGTTC 
TCGATCGTCT TTGGGGTCAT TGTATATCGG ATCACAACTG CAGCTGCTCT GTCTCTCAAC 
AAGGCCACGC GCTCCAATGT CCGGGTGACA GTCACGGCAA CAGCAGTCAT CATCAACCTT 
GTAGTCATCC TCATTCTGGA TGAGATCTAT GGGGCTGTGG CCAAGTGGCT CACCAAAATA 
GAGGTTCCAA AAACAGAACA GACTTTTGAA GAGCGCTTGA TACTCAAAGC TTTCTTGCTA 
AAGTTTGTCA ACGCCTACTC TCCCATCTTC TACGTGGCCT TTTTCAAGGG CAGGTTTGTG 
GGCAGGCCTG GAAGCTATGT CTATGTGTTT GATGGCTACC GCATGGAAGA GTGTGCTCCA 
GGGGGCTGCC TCATGGAGCT CTGCATTCAG CTCAGCATCA TCATGTTGGG AAAGCAGTTG 
ATCCAGAACA ACATCTTTGA GATTGGAGTT CCGAAACTAA AGAAACTATT TCGGAAGCTG 
AAAGATGAGA CAGAACCTGG AGAAGCTGAC CCTGAGCATT CAAAACCTCC AGAGCAGTGG 
GACTTAGACT ACAGCCTGGA GCCGTACACA GGGCTGACTC CGGAGTACAT GGAAATGATC 
ATCCAGTTCG GTTTTGTCAC TCTCTTTGTG GCCTCCTTCC CTCTGGCACC CGTGTTTGCC 
CTCCTCAACA ATGTCATTGA AGTGCGGCTA GATGCAAAAA AGTTCGTCAC GGAGCTGAGA 
CGGCCGGATG CTGTGAGAAC CAAAGATATT GGAATTTGGT TTGACATTCT TTCCGGAATT 
GGCAAGTTCT CTGTTATCAT CAACGCATTC GTCATCGCCG TCACCTCCGA CTTCATCCCC 
CGCCTGGTGT ACCAGTACTC CTACAGTCAC AATGGGACTC TGCACGGCTT TGTGAACCAC 
ACGCTGTCCT TCTTCAACGT CAGCCAGCTG AAGGAGGGGA CACAGCCAGA GAACTCACAG 
TTCGACCAGG AGGTTCAGTT CTGCAGGTTC AAGGATTACC GAGAGCCGCC ATGGGCACCG 
AACCCATATG AGTTTTCCAA ACAGTACTGG TCTGTTCTGT CTGCTCGACT GGCTTTCGTC 
ATAATCTTCC AGAACCTGGT GATGTTCCTG AGCGTCCTTG TGGACTGGAT GATTCCAGAC 
ATCCCCACGG ACATCAGCGA CCAGATCAAG AAAGAGAAGA GTTTGTTGGT GGACTTCTTC 
CTGAAAGAGG AGCACGAGAA GCTGAGGCTC ACTGATGAGC CAGCTCAGAG GAGCCAGGGA 
GGTGGGGACA GCAGCAGGAG GAGCCAGAGT GGCGCAGCCA GCTCAGCTCC CTCGGCCCTG 
AGCCCCCCTG GCAGCAGAGC GTCTTCCGGC TCCCAGCACA CCAACCTGTG A