pola2 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	pola2
Entrez Gene ID	102301672
Full Name	DNA polymerase alpha 2, accessory subunit
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005913375.2	XP_005913437.1	DNA polymerase alpha subunit B

Pathways from BioSystems
Gene Ontology

The following pola2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pola2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa01274	XM_005913375.2 Latest version!	Haplochromis burtoni polymerase (DNA directed), alpha 2, accessory subunit (pola2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pola2 ( XM_005913375.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005913437.1 Haplochromis burtoni polymerase (DNA directed), alpha 2, accessory subunit (pola2), mRNA.

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CloneID	OHa01274
Clone ID Related Accession (Same CDS sequence)	XM_005913375.2
Accession Version	XM_005913375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	DNA polymerase alpha subunit B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179404.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005913375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTAG TAACTACAGA GAGCTTAAAG ACTGAGCTGG AAATATTCGA TATACCCTAT 
CAAGATGATT CCGTCCTCGA TAAAATGGTG GAGCAGTGTA TTTGTAACAG ACTGCAGGCA 
CAAGAGATAC TGCATGCGTG GGTTGCCTAT AGCGCCACAA AACAAGGCGT GAAGCTCACC 
ACGGACACTC TGGAGCAGTT TGAACATGAG GTTTTGAACA AGAGGATCAA ATCCAGAAAA 
ACCTCCCGAA AGGAAGAAGC AGCCAGTAGA ATGAGAAACA TTAGCATGAT TGATGATTTA 
ATAAAAGCTG AGGAAGAGGA GGAGGATCTC TTAGATTCTT ACTCTACTCC TGCTAAGGGC 
TCTCAGAAAC GACCTCTGAC GACACCAGAG CGTCCTCATT CAAAGAGAAC TGCAGCTCTG 
ATGGCCAGCC CGAGCATACT GCTGTCTCCA GCCAGCTTCT CTCCCAATGC GACACCCTCA 
CAGAAGTACA GCCAGCGGGG AGGAAAGGGA GAGGTGGTGG CCACGTTTGG GGCTGTGCAG 
GGCACCCGTT GGGTGGGCAG GAAAGATCCA GGTGCAGGCG TCCAAGTTGA GATATTGGAA 
GGACCGGAAG ATTCCCTTAA CAGCAGCTAC AAATACATGT TCCAGAGGCT GCGAGATGTT 
CGAAACGTGT TGACAGAGAA GATTGAGGAG CTAGGAGAAG GACTCAGGAC TCACTTCAGC 
ATTGAGGAGT TCTCCCCCGT CTCTCTGCCT GCCCAGGACA GTATAACTGT GCTAGGACAG 
ATTTGCTGTG ACAGTAATGG CAAACTGAAT GCACAGTCAG TTCTGCTAGA GGCTGGACCA 
GAACAAGGCG GTCAGCAAGT ACCTGTTGAC CTATCGGAGC TTAAAGAGTA TTCCCTTTTT 
CCTGGTCAGG TGGTGGTGAT GGAGGGTATG AACACTACAG GAAGAAAACT GGTGGCTTCC 
AAATTCTACG AGGCTGTTCC TCTGCCGTTC TACAGTTCTG ACATGAAAAT GGAGACAGAT 
GAGGAGCCAC TGAATGTACT GGTGGCCTGT GGACCGTACG CTCCCTCTGA CAGTCTGACC 
TTTGACCCCC TGCTGGACTT AATCAATGTT ATTGTCAGGG ATCGTCCTGA TGTCTGCCTG 
CTGCTGGGCC CATTTGTGGA TTCCAAACAT GAACAGATTG AGAAAGCCCA GGTGACTGAC 
ACTTTTGAGA ACATTTTCAC CAGATGCATT GAAAGCATTG TGGATGGGAC CAGAAGTGTT 
GGCTGTCAGC TGGTGTTTGT GCCTTCTCAG AGAGACATCC ACCACCATTT CATCTACCCA 
CAGCCTCCGT TCAGCCTGCC CCACCCCAGC AAAGAACAGA CCCAGCGTGT CACCCTGGTC 
CCTGACCCCT GCACCCTTGT GATTGACGGG GTGACTATTG GCTTGACATC AACTGACATC 
CTCATCCACA TGGGTGCAGA GGAAATCAGC TGTGGAAGCG GCTCAGACAA ATTCTCACGT 
ATACTGAAGC ACATGCTCAC ACAGCGGAGT TACTACCCAC TGTATCCGCC TGCGGACGAG 
GTAAATATGG ATTATGAGAA GTTCCAGAGC TTCGGCCAAA TGCCGCTCAC CCCCGACATC 
CTTGTTGTTC CCTCTGAAAT GCGCTACTTT GTAAAGGACG TGGTCGGCTG TGTGTGTGTC 
AACCCTGGAC GGCTGACCAA AGGCCAGGTG GGCGGGACGT ACGGGCGGCT GCTCATCCAG 
CGCAGCCCTC AGTCAGAAGA CGGGAAGAGG GTGAGCCCAT GTTTGACTGC TCAGGTGGTG 
AAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005913437.1
CDS	291..2099
Translation	MSLVTTESLKTELEIFDIPYQDDSVLDKMVEQCICNRLQAQEILHAWVAYSATKQGVKLTTDTLEQFEHEVLNKRIKSRKTSRKEEAASRMRNISMIDDLIKAEEEEEDLLDSYSTPAKGSQKRPLTTPERPHSKRTAALMASPSILLSPASFSPNATPSQKYSQRGGKGEVVATFGAVQGTRWVGRKDPGAGVQVEILEGPEDSLNSSYKYMFQRLRDVRNVLTEKIEELGEGLRTHFSIEEFSPVSLPAQDSITVLGQICCDSNGKLNAQSVLLEAGPEQGGQQVPVDLSELKEYSLFPGQVVVMEGMNTTGRKLVASKFYEAVPLPFYSSDMKMETDEEPLNVLVACGPYAPSDSLTFDPLLDLINVIVRDRPDVCLLLGPFVDSKHEQIEKAQVTDTFENIFTRCIESIVDGTRSVGCQLVFVPSQRDIHHHFIYPQPPFSLPHPSKEQTQRVTLVPDPCTLVIDGVTIGLTSTDILIHMGAEEISCGSGSDKFSRILKHMLTQRSYYPLYPPADEVNMDYEKFQSFGQMPLTPDILVVPSEMRYFVKDVVGCVCVNPGRLTKGQVGGTYGRLLIQRSPQSEDGKRVSPCLTAQVVKI

Target ORF information:

RefSeq Version	XM_005913375.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni polymerase (DNA directed), alpha 2, accessory subunit (pola2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005913375.2

ORF Insert Sequence:

ATGTCGCTAG TAACTACAGA GAGCTTAAAG ACTGAGCTGG AAATATTCGA TATACCCTAT 
CAAGATGATT CCGTCCTCGA TAAAATGGTG GAGCAGTGTA TTTGTAACAG ACTGCAGGCA 
CAAGAGATAC TGCATGCGTG GGTTGCCTAT AGCGCCACAA AACAAGGCGT GAAGCTCACC 
ACGGACACTC TGGAGCAGTT TGAACATGAG GTTTTGAACA AGAGGATCAA ATCCAGAAAA 
ACCTCCCGAA AGGAAGAAGC AGCCAGTAGA ATGAGAAACA TTAGCATGAT TGATGATTTA 
ATAAAAGCTG AGGAAGAGGA GGAGGATCTC TTAGATTCTT ACTCTACTCC TGCTAAGGGC 
TCTCAGAAAC GACCTCTGAC GACACCAGAG CGTCCTCATT CAAAGAGAAC TGCAGCTCTG 
ATGGCCAGCC CGAGCATACT GCTGTCTCCA GCCAGCTTCT CTCCCAATGC GACACCCTCA 
CAGAAGTACA GCCAGCGGGG AGGAAAGGGA GAGGTGGTGG CCACGTTTGG GGCTGTGCAG 
GGCACCCGTT GGGTGGGCAG GAAAGATCCA GGTGCAGGCG TCCAAGTTGA GATATTGGAA 
GGACCGGAAG ATTCCCTTAA CAGCAGCTAC AAATACATGT TCCAGAGGCT GCGAGATGTT 
CGAAACGTGT TGACAGAGAA GATTGAGGAG CTAGGAGAAG GACTCAGGAC TCACTTCAGC 
ATTGAGGAGT TCTCCCCCGT CTCTCTGCCT GCCCAGGACA GTATAACTGT GCTAGGACAG 
ATTTGCTGTG ACAGTAATGG CAAACTGAAT GCACAGTCAG TTCTGCTAGA GGCTGGACCA 
GAACAAGGCG GTCAGCAAGT ACCTGTTGAC CTATCGGAGC TTAAAGAGTA TTCCCTTTTT 
CCTGGTCAGG TGGTGGTGAT GGAGGGTATG AACACTACAG GAAGAAAACT GGTGGCTTCC 
AAATTCTACG AGGCTGTTCC TCTGCCGTTC TACAGTTCTG ACATGAAAAT GGAGACAGAT 
GAGGAGCCAC TGAATGTACT GGTGGCCTGT GGACCGTACG CTCCCTCTGA CAGTCTGACC 
TTTGACCCCC TGCTGGACTT AATCAATGTT ATTGTCAGGG ATCGTCCTGA TGTCTGCCTG 
CTGCTGGGCC CATTTGTGGA TTCCAAACAT GAACAGATTG AGAAAGCCCA GGTGACTGAC 
ACTTTTGAGA ACATTTTCAC CAGATGCATT GAAAGCATTG TGGATGGGAC CAGAAGTGTT 
GGCTGTCAGC TGGTGTTTGT GCCTTCTCAG AGAGACATCC ACCACCATTT CATCTACCCA 
CAGCCTCCGT TCAGCCTGCC CCACCCCAGC AAAGAACAGA CCCAGCGTGT CACCCTGGTC 
CCTGACCCCT GCACCCTTGT GATTGACGGG GTGACTATTG GCTTGACATC AACTGACATC 
CTCATCCACA TGGGTGCAGA GGAAATCAGC TGTGGAAGCG GCTCAGACAA ATTCTCACGT 
ATACTGAAGC ACATGCTCAC ACAGCGGAGT TACTACCCAC TGTATCCGCC TGCGGACGAG 
GTAAATATGG ATTATGAGAA GTTCCAGAGC TTCGGCCAAA TGCCGCTCAC CCCCGACATC 
CTTGTTGTTC CCTCTGAAAT GCGCTACTTT GTAAAGGACG TGGTCGGCTG TGTGTGTGTC 
AACCCTGGAC GGCTGACCAA AGGCCAGGTG GGCGGGACGT ACGGGCGGCT GCTCATCCAG 
CGCAGCCCTC AGTCAGAAGA CGGGAAGAGG GTGAGCCCAT GTTTGACTGC TCAGGTGGTG 
AAAATCTAA