ap4b1 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	ap4b1
Entrez Gene ID	102291440
Full Name	adaptor related protein complex 4 beta 1 subunit
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005950640.2	XP_005950702.2	AP-4 complex subunit beta-1 isoform X1
XM_005950642.2	XP_005950704.2	AP-4 complex subunit beta-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ap4b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap4b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa34687	XM_005950640.2 Latest version!	Haplochromis burtoni adaptor-related protein complex 4, beta 1 subunit (ap4b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OHa34688	XM_005950642.2 Latest version!	Haplochromis burtoni adaptor-related protein complex 4, beta 1 subunit (ap4b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ap4b1 ( XM_005950640.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005950702.2 Haplochromis burtoni adaptor-related protein complex 4, beta 1 subunit (ap4b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHa34687
Clone ID Related Accession (Same CDS sequence)	XM_005950640.2
Accession Version	XM_005950640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	AP-4 complex subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005180275.1) and transcript sequence (GBBF01067935.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005950640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 331 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCCTTATC TGGGCGGGGA GGAGACGGTG AGGGAGCTGA GGAGAGCTCT GTCCAACCCC 
AACGTCCAGT CAGACCCGCT GCGGTACAGG AACACCATCC TCAAAGTGAT CAGGACCATG 
TCACAAGGGG TAGATGTGTC TGGCCTGTTC AGTGAGATGG TGAAAGCCTG TGCCACAGTA 
GATGTAGTCC AGAAGAAATT GGTTTATGTC TTCCTGTGTT CCTACGCCAC TTTAAACCCG 
GAGTTGTCCC TGCTGGTCAT CAACACTCTG AGGAAGGACT GCCAGGATCC AAACCCAATG 
GTCCGCAGTC TAGCCCTCAG GAACATGACC AACCTCAGGT TACCCAGCCT GGTGGAGTAT 
GTGGAGCAGC CTCTAACAGC AGGACTGAGA GACAGAGCAG CCTGCGTACG ACGTGTGGCT 
GTACTCGGCT GGGCCAAATT GCACAACCTC CAACCTAGCT CTGAGATCGA TGCAGCAGTG 
GTGAATGAAC TTTACAGCCT CCTGAGGGAC CCGGACCCCG TGGTGATGGT GAATTGCCTC 
CGAGCTTTGG AGGAGATCCT GAAGGAGGAG GGTGGTATCG CTATTAACAA ACCCATTACC 
CACCACCTGC TCAACAGGCT TAAGGAGTGT GACATTTGGG GTCAGTCTGA GGTGCTGAGA 
GTTCTTCAGC GCTACAGGCC CCAGTCAGAG GACGAGCTCT TTGACATCCT GTCCCTGCTG 
GACTCGTTCC TTGTAAGCCC CCACCCCCCT GTTATGGCTG CCACCCTTAG CCTCTTCCTG 
AGCGTCTCAT CCAACCTGCC CGCCATCAGT CTGGCGGCCC TGGAACGAGT CAGTGGACCC 
CTGCTTGCAG CCTGTGGGAG TGGTTCCAGA GAGATGAGGT TTGCTGCCGT CTGCCACATA 
CAGTTGTTGC TGCGCTCTGT CCCTGGCCTG CTGGGGCCGC ACTACAAGCG TTTCTTCTGT 
GGCTATGCTG AGCCTGCCTA CATCAAAGAA AGGAAGATGC AGGGAAGGAA ACCATTATCA 
GTCCAAATCG CCATCAGCTG TTCTCACCGG CATCTCCAGC ACCGCCCCGT TGAGCCCACT 
GCACCACTCC TCCCCTGCAG ATGCGCCCGG GACCACACCT CCGCCAAACA TGTGCTGGTA 
GAGCTGGTGA ATGACGAGAA CGTAGCAATG ATACTGGATG AGCTGAAAGG ATACTGCACT 
GATGTCAACA CTGACACTGC CCAGGCTGCA ATCTCAGCAA TAGGTCGTAT TGGACGCAGC 
TACAGCGACA GATGTCTACA AATCCTCACT GGGCTGCTTG GACTCAAACA GGATCATATC 
ACTTCTGCGG TGGTGCAGAC AATGCGTGAC CTGGTCTGGG TGTGTCCCCA GTGTAGCGAC 
ACTGTGTGCC TTGCACTGGA TGGCTGCGAG GAAACACTGC AGGATATCCA GGGCAGGCAG 
GCTTTGCTCT GGTTGCTGGG TGTGTATGGG GAGCGAATTT CCACTGCCCC CTACACTTTG 
GAAGTGCTCA TCGATGGAGT GAGAAGCGAG GCTTCTCTGG GAATCAAGAT GGAGCTGCTG 
ACAGCCACCA TGAGACTTTT CCTGTGTCGG CCTGCTGAGA CACAAGACAT GCTGGGAAGA 
CTGCTGCACT ACTGCATAGA GGAGGAGACA GACATGTGCG TGCGTGATCA GGCTCTTCTC 
TACTATCGCC TGCTTCATTG TGGGATAGAA AAGACACGCG AGGTCCTTCA AGGTCGGAGG 
TCAGATCCCT CTCTGGGTGT TCTGATTGGG CGTCCCGCCA AGCCTGTCAG CCAGTGGGCA 
CGCTGCTTTA ACACGTTGGA GCCTCTGAGC CAGGGTGCTG TAGAGGCCGA GTCAGACAGC 
AGGGGAAGCC CCGAACACGT GACCTATAAC CCCAAGCCAA ATGCCGACCT ATCAGACACA 
CTGAGCTGTA GTCACATTGA GGAGGTTCAT TCAGGATCAG ACAGTGCCAT GCGGTGTTCA 
GACTCCTCCA CTAATGTCCT GGCTTCCAGC ATCGCAGCCC CTCTCAGTTT GTCCCTCTCC 
CCAGCTGTCA GCCCAGAGGA CTTTGAGCAC CTGTGGCTGC AGCGACAGGC TTTGCATACT 
GAACGCGGTG CTGAGAAAAT AGAGGAGGTT GACTGTGCGT GGATAGAAGA ATGTGTCCGG 
TGTCCAGCAG TACTTCAGTG TTCTCCCCAA AGCCTCCAAG CTGCCATGCA GCTGGTCAAT 
ATCCAGACGC TGGCGTTCAC GCCACAGCAT ATGCTGCCCT GGAGGGTCTA CCTGTACACA 
CACACCCAGC GCTCCCACTC CATGGACGCA CCCAACACTA CGCTCATACT GGGGGAGCTG 
CTATGTACTG GAGAAGCCAA TCAGAAATCA GGAGTAAAAA AAGGCACAGC TGACAGTGAA 
GCTGGAGCAG AGGGGAAAGA AGACCAACTG GTGAGAAAGG AAATGGATGC AACAGCTGAC 
AGGCTGAGGG TGAGCGAAGA TGGACAGGAA GAGGAGGAGG GAATAAAAGT GATTCTCAAT 
CAGCAACCAA AAGATGATGA CGCTCTGAGA CAGTTTCTCA CAATCCTTAT CACTGTACTG 
AACACACTGT CCTCAGAGAA AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005950702.2
CDS	219..2825
Translation	MPYLGGEETVRELRRALSNPNVQSDPLRYRNTILKVIRTMSQGVDVSGLFSEMVKACATVDVVQKKLVYVFLCSYATLNPELSLLVINTLRKDCQDPNPMVRSLALRNMTNLRLPSLVEYVEQPLTAGLRDRAACVRRVAVLGWAKLHNLQPSSEIDAAVVNELYSLLRDPDPVVMVNCLRALEEILKEEGGIAINKPITHHLLNRLKECDIWGQSEVLRVLQRYRPQSEDELFDILSLLDSFLVSPHPPVMAATLSLFLSVSSNLPAISLAALERVSGPLLAACGSGSREMRFAAVCHIQLLLRSVPGLLGPHYKRFFCGYAEPAYIKERKMQGRKPLSVQIAISCSHRHLQHRPVEPTAPLLPCRCARDHTSAKHVLVELVNDENVAMILDELKGYCTDVNTDTAQAAISAIGRIGRSYSDRCLQILTGLLGLKQDHITSAVVQTMRDLVWVCPQCSDTVCLALDGCEETLQDIQGRQALLWLLGVYGERISTAPYTLEVLIDGVRSEASLGIKMELLTATMRLFLCRPAETQDMLGRLLHYCIEEETDMCVRDQALLYYRLLHCGIEKTREVLQGRRSDPSLGVLIGRPAKPVSQWARCFNTLEPLSQGAVEAESDSRGSPEHVTYNPKPNADLSDTLSCSHIEEVHSGSDSAMRCSDSSTNVLASSIAAPLSLSLSPAVSPEDFEHLWLQRQALHTERGAEKIEEVDCAWIEECVRCPAVLQCSPQSLQAAMQLVNIQTLAFTPQHMLPWRVYLYTHTQRSHSMDAPNTTLILGELLCTGEANQKSGVKKGTADSEAGAEGKEDQLVRKEMDATADRLRVSEDGQEEEEGIKVILNQQPKDDDALRQFLTILITVLNTLSSEKD

Target ORF information:

RefSeq Version	XM_005950640.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni adaptor-related protein complex 4, beta 1 subunit (ap4b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005950640.2

ORF Insert Sequence:

ATGCCTTATC TGGGCGGGGA GGAGACGGTG AGGGAGCTGA GGAGAGCTCT GTCCAACCCC 
AACGTCCAGT CAGACCCGCT GCGGTACAGG AACACCATCC TCAAAGTGAT CAGGACCATG 
TCACAAGGGG TAGATGTGTC TGGCCTGTTC AGTGAGATGG TGAAAGCCTG TGCCACAGTA 
GATGTAGTCC AGAAGAAATT GGTTTATGTC TTCCTGTGTT CCTACGCCAC TTTAAACCCG 
GAGTTGTCCC TGCTGGTCAT CAACACTCTG AGGAAGGACT GCCAGGATCC AAACCCAATG 
GTCCGCAGTC TAGCCCTCAG GAACATGACC AACCTCAGGT TACCCAGCCT GGTGGAGTAT 
GTGGAGCAGC CTCTAACAGC AGGACTGAGA GACAGAGCAG CCTGCGTACG ACGTGTGGCT 
GTACTCGGCT GGGCCAAATT GCACAACCTC CAACCTAGCT CTGAGATCGA TGCAGCAGTG 
GTGAATGAAC TTTACAGCCT CCTGAGGGAC CCGGACCCCG TGGTGATGGT GAATTGCCTC 
CGAGCTTTGG AGGAGATCCT GAAGGAGGAG GGTGGTATCG CTATTAACAA ACCCATTACC 
CACCACCTGC TCAACAGGCT TAAGGAGTGT GACATTTGGG GTCAGTCTGA GGTGCTGAGA 
GTTCTTCAGC GCTACAGGCC CCAGTCAGAG GACGAGCTCT TTGACATCCT GTCCCTGCTG 
GACTCGTTCC TTGTAAGCCC CCACCCCCCT GTTATGGCTG CCACCCTTAG CCTCTTCCTG 
AGCGTCTCAT CCAACCTGCC CGCCATCAGT CTGGCGGCCC TGGAACGAGT CAGTGGACCC 
CTGCTTGCAG CCTGTGGGAG TGGTTCCAGA GAGATGAGGT TTGCTGCCGT CTGCCACATA 
CAGTTGTTGC TGCGCTCTGT CCCTGGCCTG CTGGGGCCGC ACTACAAGCG TTTCTTCTGT 
GGCTATGCTG AGCCTGCCTA CATCAAAGAA AGGAAGATGC AGGGAAGGAA ACCATTATCA 
GTCCAAATCG CCATCAGCTG TTCTCACCGG CATCTCCAGC ACCGCCCCGT TGAGCCCACT 
GCACCACTCC TCCCCTGCAG ATGCGCCCGG GACCACACCT CCGCCAAACA TGTGCTGGTA 
GAGCTGGTGA ATGACGAGAA CGTAGCAATG ATACTGGATG AGCTGAAAGG ATACTGCACT 
GATGTCAACA CTGACACTGC CCAGGCTGCA ATCTCAGCAA TAGGTCGTAT TGGACGCAGC 
TACAGCGACA GATGTCTACA AATCCTCACT GGGCTGCTTG GACTCAAACA GGATCATATC 
ACTTCTGCGG TGGTGCAGAC AATGCGTGAC CTGGTCTGGG TGTGTCCCCA GTGTAGCGAC 
ACTGTGTGCC TTGCACTGGA TGGCTGCGAG GAAACACTGC AGGATATCCA GGGCAGGCAG 
GCTTTGCTCT GGTTGCTGGG TGTGTATGGG GAGCGAATTT CCACTGCCCC CTACACTTTG 
GAAGTGCTCA TCGATGGAGT GAGAAGCGAG GCTTCTCTGG GAATCAAGAT GGAGCTGCTG 
ACAGCCACCA TGAGACTTTT CCTGTGTCGG CCTGCTGAGA CACAAGACAT GCTGGGAAGA 
CTGCTGCACT ACTGCATAGA GGAGGAGACA GACATGTGCG TGCGTGATCA GGCTCTTCTC 
TACTATCGCC TGCTTCATTG TGGGATAGAA AAGACACGCG AGGTCCTTCA AGGTCGGAGG 
TCAGATCCCT CTCTGGGTGT TCTGATTGGG CGTCCCGCCA AGCCTGTCAG CCAGTGGGCA 
CGCTGCTTTA ACACGTTGGA GCCTCTGAGC CAGGGTGCTG TAGAGGCCGA GTCAGACAGC 
AGGGGAAGCC CCGAACACGT GACCTATAAC CCCAAGCCAA ATGCCGACCT ATCAGACACA 
CTGAGCTGTA GTCACATTGA GGAGGTTCAT TCAGGATCAG ACAGTGCCAT GCGGTGTTCA 
GACTCCTCCA CTAATGTCCT GGCTTCCAGC ATCGCAGCCC CTCTCAGTTT GTCCCTCTCC 
CCAGCTGTCA GCCCAGAGGA CTTTGAGCAC CTGTGGCTGC AGCGACAGGC TTTGCATACT 
GAACGCGGTG CTGAGAAAAT AGAGGAGGTT GACTGTGCGT GGATAGAAGA ATGTGTCCGG 
TGTCCAGCAG TACTTCAGTG TTCTCCCCAA AGCCTCCAAG CTGCCATGCA GCTGGTCAAT 
ATCCAGACGC TGGCGTTCAC GCCACAGCAT ATGCTGCCCT GGAGGGTCTA CCTGTACACA 
CACACCCAGC GCTCCCACTC CATGGACGCA CCCAACACTA CGCTCATACT GGGGGAGCTG 
CTATGTACTG GAGAAGCCAA TCAGAAATCA GGAGTAAAAA AAGGCACAGC TGACAGTGAA 
GCTGGAGCAG AGGGGAAAGA AGACCAACTG GTGAGAAAGG AAATGGATGC AACAGCTGAC 
AGGCTGAGGG TGAGCGAAGA TGGACAGGAA GAGGAGGAGG GAATAAAAGT GATTCTCAAT 
CAGCAACCAA AAGATGATGA CGCTCTGAGA CAGTTTCTCA CAATCCTTAT CACTGTACTG 
AACACACTGT CCTCAGAGAA AGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap4b1 ( XM_005950642.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005950704.2 Haplochromis burtoni adaptor-related protein complex 4, beta 1 subunit (ap4b1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHa34688
Clone ID Related Accession (Same CDS sequence)	XM_005950642.2
Accession Version	XM_005950642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	AP-4 complex subunit beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005180275.1) and transcript sequence (GBBF01067935.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005950642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 331 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCCTTATC TGGGCGGGGA GGAGACGGTG AGGGAGCTGA GGAGAGCTCT GTCCAACCCC 
AACGTCCAGT CAGACCCGCT GCGGTACAGG AACACCATCC TCAAAGTGAT CAGGACCATG 
TCACAAGGGG TAGATGTGTC TGGCCTGTTC AGTGAGATGG TGAAAGCCTG TGCCACAGTA 
GATGTAGTCC AGAAGAAATT GGTTTATGTC TTCCTGTGTT CCTACGCCAC TTTAAACCCG 
GAGTTGTCCC TGCTGGTCAT CAACACTCTG AGGAAGGACT GCCAGGATCC AAACCCAATG 
GTCCGCAGTC TAGCCCTCAG GAACATGACC AACCTCAGGT TACCCAGCCT GGTGGAGTAT 
GTGGAGCAGC CTCTAACAGC AGGACTGAGA GACAGAGCAG CCTGCGTACG ACGTGTGGCT 
GTACTCGGCT GGGCCAAATT GCACAACCTC CAACCTAGCT CTGAGATCGA TGCAGCAGTG 
GTGAATGAAC TTTACAGCCT CCTGAGGGAC CCGGACCCCG TGGTGATGGT GAATTGCCTC 
CGAGCTTTGG AGGAGATCCT GAAGGAGGAG GGTGGTATCG CTATTAACAA ACCCATTACC 
CACCACCTGC TCAACAGGCT TAAGGAGTGT GACATTTGGG GTCAGTCTGA GGTGCTGAGA 
GTTCTTCAGC GCTACAGGCC CCAGTCAGAG GACGAGCTCT TTGACATCCT GTCCCTGCTG 
GACTCGTTCC TTGTAAGCCC CCACCCCCCT GTTATGGCTG CCACCCTTAG CCTCTTCCTG 
AGCGTCTCAT CCAACCTGCC CGCCATCAGT CTGGCGGCCC TGGAACGAGT CAGTGGACCC 
CTGCTTGCAG CCTGTGGGAG TGGTTCCAGA GAGATGAGGT TTGCTGCCGT CTGCCACATA 
CAGTTGTTGC TGCGCTCTGT CCCTGGCCTG CTGGGGCCGC ACTACAAGCG TTTCTTCTGT 
GGCTATGCTG AGCCTGCCTA CATCAAAGAA AGGAAGATGC AGGTGCTGGT AGAGCTGGTG 
AATGACGAGA ACGTAGCAAT GATACTGGAT GAGCTGAAAG GATACTGCAC TGATGTCAAC 
ACTGACACTG CCCAGGCTGC AATCTCAGCA ATAGGTCGTA TTGGACGCAG CTACAGCGAC 
AGATGTCTAC AAATCCTCAC TGGGCTGCTT GGACTCAAAC AGGATCATAT CACTTCTGCG 
GTGGTGCAGA CAATGCGTGA CCTGGTCTGG GTGTGTCCCC AGTGTAGCGA CACTGTGTGC 
CTTGCACTGG ATGGCTGCGA GGAAACACTG CAGGATATCC AGGGCAGGCA GGCTTTGCTC 
TGGTTGCTGG GTGTGTATGG GGAGCGAATT TCCACTGCCC CCTACACTTT GGAAGTGCTC 
ATCGATGGAG TGAGAAGCGA GGCTTCTCTG GGAATCAAGA TGGAGCTGCT GACAGCCACC 
ATGAGACTTT TCCTGTGTCG GCCTGCTGAG ACACAAGACA TGCTGGGAAG ACTGCTGCAC 
TACTGCATAG AGGAGGAGAC AGACATGTGC GTGCGTGATC AGGCTCTTCT CTACTATCGC 
CTGCTTCATT GTGGGATAGA AAAGACACGC GAGGTCCTTC AAGGTCGGAG GTCAGATCCC 
TCTCTGGGTG TTCTGATTGG GCGTCCCGCC AAGCCTGTCA GCCAGTGGGC ACGCTGCTTT 
AACACGTTGG AGCCTCTGAG CCAGGGTGCT GTAGAGGCCG AGTCAGACAG CAGGGGAAGC 
CCCGAACACG TGACCTATAA CCCCAAGCCA AATGCCGACC TATCAGACAC ACTGAGCTGT 
AGTCACATTG AGGAGGTTCA TTCAGGATCA GACAGTGCCA TGCGGTGTTC AGACTCCTCC 
ACTAATGTCC TGGCTTCCAG CATCGCAGCC CCTCTCAGTT TGTCCCTCTC CCCAGCTGTC 
AGCCCAGAGG ACTTTGAGCA CCTGTGGCTG CAGCGACAGG CTTTGCATAC TGAACGCGGT 
GCTGAGAAAA TAGAGGAGGT TGACTGTGCG TGGATAGAAG AATGTGTCCG GTGTCCAGCA 
GTACTTCAGT GTTCTCCCCA AAGCCTCCAA GCTGCCATGC AGCTGGTCAA TATCCAGACG 
CTGGCGTTCA CGCCACAGCA TATGCTGCCC TGGAGGGTCT ACCTGTACAC ACACACCCAG 
CGCTCCCACT CCATGGACGC ACCCAACACT ACGCTCATAC TGGGGGAGCT GCTATGTACT 
GGAGAAGCCA ATCAGAAATC AGGAGTAAAA AAAGGCACAG CTGACAGTGA AGCTGGAGCA 
GAGGGGAAAG AAGACCAACT GGTGAGAAAG GAAATGGATG CAACAGCTGA CAGGCTGAGG 
GTGAGCGAAG ATGGACAGGA AGAGGAGGAG GGAATAAAAG TGATTCTCAA TCAGCAACCA 
AAAGATGATG ACGCTCTGAG ACAGTTTCTC ACAATCCTTA TCACTGTACT GAACACACTG 
TCCTCAGAGA AAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005950704.2
CDS	219..2696
Translation	MPYLGGEETVRELRRALSNPNVQSDPLRYRNTILKVIRTMSQGVDVSGLFSEMVKACATVDVVQKKLVYVFLCSYATLNPELSLLVINTLRKDCQDPNPMVRSLALRNMTNLRLPSLVEYVEQPLTAGLRDRAACVRRVAVLGWAKLHNLQPSSEIDAAVVNELYSLLRDPDPVVMVNCLRALEEILKEEGGIAINKPITHHLLNRLKECDIWGQSEVLRVLQRYRPQSEDELFDILSLLDSFLVSPHPPVMAATLSLFLSVSSNLPAISLAALERVSGPLLAACGSGSREMRFAAVCHIQLLLRSVPGLLGPHYKRFFCGYAEPAYIKERKMQVLVELVNDENVAMILDELKGYCTDVNTDTAQAAISAIGRIGRSYSDRCLQILTGLLGLKQDHITSAVVQTMRDLVWVCPQCSDTVCLALDGCEETLQDIQGRQALLWLLGVYGERISTAPYTLEVLIDGVRSEASLGIKMELLTATMRLFLCRPAETQDMLGRLLHYCIEEETDMCVRDQALLYYRLLHCGIEKTREVLQGRRSDPSLGVLIGRPAKPVSQWARCFNTLEPLSQGAVEAESDSRGSPEHVTYNPKPNADLSDTLSCSHIEEVHSGSDSAMRCSDSSTNVLASSIAAPLSLSLSPAVSPEDFEHLWLQRQALHTERGAEKIEEVDCAWIEECVRCPAVLQCSPQSLQAAMQLVNIQTLAFTPQHMLPWRVYLYTHTQRSHSMDAPNTTLILGELLCTGEANQKSGVKKGTADSEAGAEGKEDQLVRKEMDATADRLRVSEDGQEEEEGIKVILNQQPKDDDALRQFLTILITVLNTLSSEKD

Target ORF information:

RefSeq Version	XM_005950642.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni adaptor-related protein complex 4, beta 1 subunit (ap4b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005950642.2

ORF Insert Sequence:

ATGCCTTATC TGGGCGGGGA GGAGACGGTG AGGGAGCTGA GGAGAGCTCT GTCCAACCCC 
AACGTCCAGT CAGACCCGCT GCGGTACAGG AACACCATCC TCAAAGTGAT CAGGACCATG 
TCACAAGGGG TAGATGTGTC TGGCCTGTTC AGTGAGATGG TGAAAGCCTG TGCCACAGTA 
GATGTAGTCC AGAAGAAATT GGTTTATGTC TTCCTGTGTT CCTACGCCAC TTTAAACCCG 
GAGTTGTCCC TGCTGGTCAT CAACACTCTG AGGAAGGACT GCCAGGATCC AAACCCAATG 
GTCCGCAGTC TAGCCCTCAG GAACATGACC AACCTCAGGT TACCCAGCCT GGTGGAGTAT 
GTGGAGCAGC CTCTAACAGC AGGACTGAGA GACAGAGCAG CCTGCGTACG ACGTGTGGCT 
GTACTCGGCT GGGCCAAATT GCACAACCTC CAACCTAGCT CTGAGATCGA TGCAGCAGTG 
GTGAATGAAC TTTACAGCCT CCTGAGGGAC CCGGACCCCG TGGTGATGGT GAATTGCCTC 
CGAGCTTTGG AGGAGATCCT GAAGGAGGAG GGTGGTATCG CTATTAACAA ACCCATTACC 
CACCACCTGC TCAACAGGCT TAAGGAGTGT GACATTTGGG GTCAGTCTGA GGTGCTGAGA 
GTTCTTCAGC GCTACAGGCC CCAGTCAGAG GACGAGCTCT TTGACATCCT GTCCCTGCTG 
GACTCGTTCC TTGTAAGCCC CCACCCCCCT GTTATGGCTG CCACCCTTAG CCTCTTCCTG 
AGCGTCTCAT CCAACCTGCC CGCCATCAGT CTGGCGGCCC TGGAACGAGT CAGTGGACCC 
CTGCTTGCAG CCTGTGGGAG TGGTTCCAGA GAGATGAGGT TTGCTGCCGT CTGCCACATA 
CAGTTGTTGC TGCGCTCTGT CCCTGGCCTG CTGGGGCCGC ACTACAAGCG TTTCTTCTGT 
GGCTATGCTG AGCCTGCCTA CATCAAAGAA AGGAAGATGC AGGTGCTGGT AGAGCTGGTG 
AATGACGAGA ACGTAGCAAT GATACTGGAT GAGCTGAAAG GATACTGCAC TGATGTCAAC 
ACTGACACTG CCCAGGCTGC AATCTCAGCA ATAGGTCGTA TTGGACGCAG CTACAGCGAC 
AGATGTCTAC AAATCCTCAC TGGGCTGCTT GGACTCAAAC AGGATCATAT CACTTCTGCG 
GTGGTGCAGA CAATGCGTGA CCTGGTCTGG GTGTGTCCCC AGTGTAGCGA CACTGTGTGC 
CTTGCACTGG ATGGCTGCGA GGAAACACTG CAGGATATCC AGGGCAGGCA GGCTTTGCTC 
TGGTTGCTGG GTGTGTATGG GGAGCGAATT TCCACTGCCC CCTACACTTT GGAAGTGCTC 
ATCGATGGAG TGAGAAGCGA GGCTTCTCTG GGAATCAAGA TGGAGCTGCT GACAGCCACC 
ATGAGACTTT TCCTGTGTCG GCCTGCTGAG ACACAAGACA TGCTGGGAAG ACTGCTGCAC 
TACTGCATAG AGGAGGAGAC AGACATGTGC GTGCGTGATC AGGCTCTTCT CTACTATCGC 
CTGCTTCATT GTGGGATAGA AAAGACACGC GAGGTCCTTC AAGGTCGGAG GTCAGATCCC 
TCTCTGGGTG TTCTGATTGG GCGTCCCGCC AAGCCTGTCA GCCAGTGGGC ACGCTGCTTT 
AACACGTTGG AGCCTCTGAG CCAGGGTGCT GTAGAGGCCG AGTCAGACAG CAGGGGAAGC 
CCCGAACACG TGACCTATAA CCCCAAGCCA AATGCCGACC TATCAGACAC ACTGAGCTGT 
AGTCACATTG AGGAGGTTCA TTCAGGATCA GACAGTGCCA TGCGGTGTTC AGACTCCTCC 
ACTAATGTCC TGGCTTCCAG CATCGCAGCC CCTCTCAGTT TGTCCCTCTC CCCAGCTGTC 
AGCCCAGAGG ACTTTGAGCA CCTGTGGCTG CAGCGACAGG CTTTGCATAC TGAACGCGGT 
GCTGAGAAAA TAGAGGAGGT TGACTGTGCG TGGATAGAAG AATGTGTCCG GTGTCCAGCA 
GTACTTCAGT GTTCTCCCCA AAGCCTCCAA GCTGCCATGC AGCTGGTCAA TATCCAGACG 
CTGGCGTTCA CGCCACAGCA TATGCTGCCC TGGAGGGTCT ACCTGTACAC ACACACCCAG 
CGCTCCCACT CCATGGACGC ACCCAACACT ACGCTCATAC TGGGGGAGCT GCTATGTACT 
GGAGAAGCCA ATCAGAAATC AGGAGTAAAA AAAGGCACAG CTGACAGTGA AGCTGGAGCA 
GAGGGGAAAG AAGACCAACT GGTGAGAAAG GAAATGGATG CAACAGCTGA CAGGCTGAGG 
GTGAGCGAAG ATGGACAGGA AGAGGAGGAG GGAATAAAAG TGATTCTCAA TCAGCAACCA 
AAAGATGATG ACGCTCTGAG ACAGTTTCTC ACAATCCTTA TCACTGTACT GAACACACTG 
TCCTCAGAGA AAGATTAA