COL25A1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	COL25A1
Entrez Gene ID	107123084
Full Name	collagen type XXV alpha 1 chain
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015426262.1	XP_015281748.1	collagen alpha-1(XXV) chain

Pathways from BioSystems
Gene Ontology

The following COL25A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL25A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe17260	XM_015426262.1 Latest version!	Gekko japonicus collagen, type XXV, alpha 1 (COL25A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL25A1 ( XM_015426262.1 ) cDNA ORF clone, Gekko japonicus -> XP_015281748.1 Gekko japonicus collagen, type XXV, alpha 1 (COL25A1), mRNA.

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CloneID	OGe17260
Clone ID Related Accession (Same CDS sequence)	XM_015426262.1
Accession Version	XM_015426262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	collagen alpha-1(XXV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015161407.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

ATGGTGGGGA AAACAAGCCA GGAAGCCAAG GGTCAGGATG CCAAGAGCGA GACCAGCTGC 
TTTGGAGGTC AGCGCTGCTG TTGGGGCAGG ACTGCGCCCT CCAGCTGGCT GCTGGCTGCC 
TTCCTCCTCT CCGTGGTATC CATCGCTTCC TGTGTGTATC TGGGGATGAA GACCAGTGAC 
CTTCAGGCCA GGGTCTTGGC CATTGAGACC GCCCAAGGGG ACTTGTCTGC TGCTTCTTAC 
CAGCTGCTGC CCAGCTATTC CTTGGATCAG CTGAATTCCT GGATGCAGGA AAAAGTGGAG 
CGACTTCTGG CTCAGAAGTC CTATGAGCAT ATGGCAAAAA TCAGAATAGC GAGAGAAGTT 
CCTTCCGAAT GCAACTGTCC ACCAGGTCCT CCTGGTAAAC GAGGTAAGCG AGGAAGAAGA 
GGAGAGATTG GACCCCCTGG TCAACCTGGT CCTCAAGGCC CACCCGGTCC AAAAGGGGAG 
AAGGGAGATC AAGGTGATCA GGGCCCTCGG ATGGTGTTTC CTAAAATCAA TCATGGGTTT 
CTCTCTGCTG ATCAGCAGCT CATTAAACGC CGCCTCATTA AGGGAGACCA AGGGCAAGCT 
GGACCCCCTG GGCCGCCTGG ACCTCCAGGG CCAAGAGGTC CTCCTGGAGA CACTGGGAAG 
GATGGCCCCC GAGGGATGCC TGGTGTACCG GGAGAGCCAG GAAAGCCAGG GGAACCAGGC 
CTGATGGGAC CTCAGGGGCC TCCGGGACAA AAGGGCTCTG TCGGTGCAAC TGGTGTCCCA 
GGAATGGACG GACAACAGGG TGAACCTGGT GTAATTGGAG AGAGAGGACT TCCTGGGCTT 
AAGGCACCTG AACATATTGG AGTTGGTTTC ATCCTCATAC GGTTTAGAAT TCCAGTTTTG 
GGGAGATTCA AGTACCCTTT GAATGAAGGC TTTGCAAGCC TGGAAACTTT CAGTGATGTT 
CTGAGCAAAA AGGAAGAAGG GGGATGGCAG GGTGATCCGG GTGAAGCTGG GCCGAAAGGT 
GATGCAGGAG AGAAGGGTGA TCCTGGGTCT TCTGCTGCAG GAATAAAGGG GGAACCTGGA 
GAATCAGGTC GACCAGGACA AAAGGGTGAA CCTGGACTCC CGGGGCTTCC TGGACTACCT 
GGTATTAAGG GTGAACCTGG TTTCATTGGC CCACAGGGAG AACCTGGATT ACCAGGGTTG 
CCTGGGACAA AGGGTGACAA GGGAGAAGCA GGGCCTCCTG GGAAAGGTGA ACGAGGTGAA 
CCGGGAGTTC CAGGGCCAAA GGGGAGGTCA GGTGAATCTG GAGCTAGAGG CCCAAAGGGG 
TCGAAGGGTG ATCCAGGAGA CAAAGGTGAT TTGGGTTCTG TAGGTCCAAA GGGCCCACCT 
GGACAGAAGG GTGATCAAGG TGCCACAGAA ATCATCGACT ACAATGGCAA TATCCATGAA 
GCCTTACAGA GGATTACCAC CCTAACTGTC ACGGGTCCTC CTGGCCCTCC TGGCCCCCAG 
GGATTGCAAG GGTCAAAGGG TGAGCCAGGA TCCCCAGGAA TACCAGGAGC TGATGGGGAG 
CAGGGGCCTA AAGGTTCAAA AGGGGATACA GGTGACCCTG GAATACCAGG AGAAAAAGGA 
GGGATTGGAC TTCCAGGACT GCCAGGATCC AATGGCATGA AAGGAGACAA AGGAGATGTT 
GGCTTGCCAG GTCCCCAAGG TCCTTCTATT ATAGGACCGC CAGGACCTCC TGGCCCGCAT 
GGTCCACCGG GGCCTATGGG ACCTCATGGA CTACCAGGCC CAAAGGGAGC ATCTGGCTTA 
GACGGAAAGC CAGGACCCCG GGGTGCAGAT GGCCCAGTTG GTCCACATGG TCTTGCAGGT 
CCAAAAGGAG ACAGAGGTGA AAAGGGAGTT CCGGGAGATC CTGGGCCCAG AGGACCATAT 
GGCTTGCCTG GCAAAGATGG AGAACCTGGC CTTGATGGTT TCCCTGGTCC TCGTGGAGAA 
AAGGGTGATG TAGGAGAAAA GGGAGAAAAG GGATTCCGTG GAGTTAAGGG GGAAAAAGGG 
GAGCCAGGCC AGCCTGGCCT GGATGGGCTG GATGCCCCTT GCCAATTGGG GCCAGATGGG 
TTACCAATGC CTGGCTGTTG GCAAAAGTTT TCAGGACTTT TATTCAACTT TAAAGACAAG 
AAAACTAAAA TTGACTCTGT CCAAAAAGCC AAGGACTTTT TGAACCAACA ACAAAATCTG 
AACCAAGATG ATAAATCAAC AGTGGATGAT CTGGAAGATG AAGGAACTAG TGACAGAGAA 
ATAGATGATA CTTCAAGTGA CTCTAGCAGT ACCACGGGAA CTTCTGAGTC GAAGAAAGGA 
GAAGCAGCAC CTAAGATGTT AAAAATGAAT TTGAAACACA AGAAGAAGAA GAAACGTAAG 
AGAGAAACTG AACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015281748.1
CDS	1..2418
Translation	MVGKTSQEAKGQDAKSETSCFGGQRCCWGRTAPSSWLLAAFLLSVVSIASCVYLGMKTSDLQARVLAIETAQGDLSAASYQLLPSYSLDQLNSWMQEKVERLLAQKSYEHMAKIRIAREVPSECNCPPGPPGKRGKRGRRGEIGPPGQPGPQGPPGPKGEKGDQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPGDTGKDGPRGMPGVPGEPGKPGEPGLMGPQGPPGQKGSVGATGVPGMDGQQGEPGVIGERGLPGLKAPEHIGVGFILIRFRIPVLGRFKYPLNEGFASLETFSDVLSKKEEGGWQGDPGEAGPKGDAGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGDKGEAGPPGKGERGEPGVPGPKGRSGESGARGPKGSKGDPGDKGDLGSVGPKGPPGQKGDQGATEIIDYNGNIHEALQRITTLTVTGPPGPPGPQGLQGSKGEPGSPGIPGADGEQGPKGSKGDTGDPGIPGEKGGIGLPGLPGSNGMKGDKGDVGLPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGASGLDGKPGPRGADGPVGPHGLAGPKGDRGEKGVPGDPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDVGEKGEKGFRGVKGEKGEPGQPGLDGLDAPCQLGPDGLPMPGCWQKFSGLLFNFKDKKTKIDSVQKAKDFLNQQQNLNQDDKSTVDDLEDEGTSDREIDDTSSDSSSTTGTSESKKGEAAPKMLKMNLKHKKKKKRKRETEL

Target ORF information:

RefSeq Version	XM_015426262.1
Organism	Gekko japonicus
Definition	Gekko japonicus collagen, type XXV, alpha 1 (COL25A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015426262.1

ORF Insert Sequence:

ATGGTGGGGA AAACAAGCCA GGAAGCCAAG GGTCAGGATG CCAAGAGCGA GACCAGCTGC 
TTTGGAGGTC AGCGCTGCTG TTGGGGCAGG ACTGCGCCCT CCAGCTGGCT GCTGGCTGCC 
TTCCTCCTCT CCGTGGTATC CATCGCTTCC TGTGTGTATC TGGGGATGAA GACCAGTGAC 
CTTCAGGCCA GGGTCTTGGC CATTGAGACC GCCCAAGGGG ACTTGTCTGC TGCTTCTTAC 
CAGCTGCTGC CCAGCTATTC CTTGGATCAG CTGAATTCCT GGATGCAGGA AAAAGTGGAG 
CGACTTCTGG CTCAGAAGTC CTATGAGCAT ATGGCAAAAA TCAGAATAGC GAGAGAAGTT 
CCTTCCGAAT GCAACTGTCC ACCAGGTCCT CCTGGTAAAC GAGGTAAGCG AGGAAGAAGA 
GGAGAGATTG GACCCCCTGG TCAACCTGGT CCTCAAGGCC CACCCGGTCC AAAAGGGGAG 
AAGGGAGATC AAGGTGATCA GGGCCCTCGG ATGGTGTTTC CTAAAATCAA TCATGGGTTT 
CTCTCTGCTG ATCAGCAGCT CATTAAACGC CGCCTCATTA AGGGAGACCA AGGGCAAGCT 
GGACCCCCTG GGCCGCCTGG ACCTCCAGGG CCAAGAGGTC CTCCTGGAGA CACTGGGAAG 
GATGGCCCCC GAGGGATGCC TGGTGTACCG GGAGAGCCAG GAAAGCCAGG GGAACCAGGC 
CTGATGGGAC CTCAGGGGCC TCCGGGACAA AAGGGCTCTG TCGGTGCAAC TGGTGTCCCA 
GGAATGGACG GACAACAGGG TGAACCTGGT GTAATTGGAG AGAGAGGACT TCCTGGGCTT 
AAGGCACCTG AACATATTGG AGTTGGTTTC ATCCTCATAC GGTTTAGAAT TCCAGTTTTG 
GGGAGATTCA AGTACCCTTT GAATGAAGGC TTTGCAAGCC TGGAAACTTT CAGTGATGTT 
CTGAGCAAAA AGGAAGAAGG GGGATGGCAG GGTGATCCGG GTGAAGCTGG GCCGAAAGGT 
GATGCAGGAG AGAAGGGTGA TCCTGGGTCT TCTGCTGCAG GAATAAAGGG GGAACCTGGA 
GAATCAGGTC GACCAGGACA AAAGGGTGAA CCTGGACTCC CGGGGCTTCC TGGACTACCT 
GGTATTAAGG GTGAACCTGG TTTCATTGGC CCACAGGGAG AACCTGGATT ACCAGGGTTG 
CCTGGGACAA AGGGTGACAA GGGAGAAGCA GGGCCTCCTG GGAAAGGTGA ACGAGGTGAA 
CCGGGAGTTC CAGGGCCAAA GGGGAGGTCA GGTGAATCTG GAGCTAGAGG CCCAAAGGGG 
TCGAAGGGTG ATCCAGGAGA CAAAGGTGAT TTGGGTTCTG TAGGTCCAAA GGGCCCACCT 
GGACAGAAGG GTGATCAAGG TGCCACAGAA ATCATCGACT ACAATGGCAA TATCCATGAA 
GCCTTACAGA GGATTACCAC CCTAACTGTC ACGGGTCCTC CTGGCCCTCC TGGCCCCCAG 
GGATTGCAAG GGTCAAAGGG TGAGCCAGGA TCCCCAGGAA TACCAGGAGC TGATGGGGAG 
CAGGGGCCTA AAGGTTCAAA AGGGGATACA GGTGACCCTG GAATACCAGG AGAAAAAGGA 
GGGATTGGAC TTCCAGGACT GCCAGGATCC AATGGCATGA AAGGAGACAA AGGAGATGTT 
GGCTTGCCAG GTCCCCAAGG TCCTTCTATT ATAGGACCGC CAGGACCTCC TGGCCCGCAT 
GGTCCACCGG GGCCTATGGG ACCTCATGGA CTACCAGGCC CAAAGGGAGC ATCTGGCTTA 
GACGGAAAGC CAGGACCCCG GGGTGCAGAT GGCCCAGTTG GTCCACATGG TCTTGCAGGT 
CCAAAAGGAG ACAGAGGTGA AAAGGGAGTT CCGGGAGATC CTGGGCCCAG AGGACCATAT 
GGCTTGCCTG GCAAAGATGG AGAACCTGGC CTTGATGGTT TCCCTGGTCC TCGTGGAGAA 
AAGGGTGATG TAGGAGAAAA GGGAGAAAAG GGATTCCGTG GAGTTAAGGG GGAAAAAGGG 
GAGCCAGGCC AGCCTGGCCT GGATGGGCTG GATGCCCCTT GCCAATTGGG GCCAGATGGG 
TTACCAATGC CTGGCTGTTG GCAAAAGTTT TCAGGACTTT TATTCAACTT TAAAGACAAG 
AAAACTAAAA TTGACTCTGT CCAAAAAGCC AAGGACTTTT TGAACCAACA ACAAAATCTG 
AACCAAGATG ATAAATCAAC AGTGGATGAT CTGGAAGATG AAGGAACTAG TGACAGAGAA 
ATAGATGATA CTTCAAGTGA CTCTAGCAGT ACCACGGGAA CTTCTGAGTC GAAGAAAGGA 
GAAGCAGCAC CTAAGATGTT AAAAATGAAT TTGAAACACA AGAAGAAGAA GAAACGTAAG 
AGAGAAACTG AACTTTAA