AP4E1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	AP4E1
Entrez Gene ID	107122387
Full Name	adaptor related protein complex 4 epsilon 1 subunit
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015425476.1	XP_015280962.1	LOW QUALITY PROTEIN: AP-4 complex subunit epsilon-1

Pathways from BioSystems
Gene Ontology

The following AP4E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP4E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe33513	XM_015425476.1 Latest version!	Gekko japonicus adaptor related protein complex 4 epsilon 1 subunit (AP4E1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP4E1 ( XM_015425476.1 ) cDNA ORF clone, Gekko japonicus -> XP_015280962.1 Gekko japonicus adaptor related protein complex 4 epsilon 1 subunit (AP4E1), mRNA.

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CloneID	OGe33513
Clone ID Related Accession (Same CDS sequence)	XM_015425476.1
Accession Version	XM_015425476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	LOW QUALITY PROTEIN: AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015175369.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTATACAA GTGCTAAAAT CCACCTTGGC ATGATGACAG AGAGGTTTAA ATGCAGACTG 
CACCGAGGGA AGATCTGTGG AGGCGGCGGC GGCGCGGCAG GAGGAGGAGG AGGGCCCGGC 
AGGGGCTCGG CCACCTCGCG GCTCGGCGGG CTCATCCGGA GCATCACCGC GCTCACCTCC 
AAGCATGAGG AAGAAAAATT AATCCAACAG GAATTAACTA GCTTGAAAGC AACAGTTTCT 
GCACCCACTA CAACCCTGAG GCTGATGAAG GAATGCATGG TGAGGCTTAT TTATTGTGAG 
ATGCTGGGCT ATGAGGCCTC GTTTGGATAC ATCCACGCTA TCAAGCTGGC CCAACAAGGA 
AACCTTTTTG AAAAAAGAGT CGGCTACTTG GCAGTTTCTT TGTTTCTTCA TGAGAATCAC 
GAGCTGTTGC TTTTGCTTGT GAACACCGTA GTGAAGGATT TGCAGAGCAC TAACCTCATG 
GAAGTATGCA TGGCTCTTAC TGTGGCAAGT CAGATATTTC CACGGGAAAT GATTCCTGCT 
NAACTTCCGC TAGTGGAAGA CAAGCTCCAG CATTCAAAGG AAATCATTAG AAGGAAAGCA 
GTTCAAGCAC TGTACAAGTT CTACCTTATT GCTCCCAACC AAGTCCAGCA CATCCATAAC 
AAATTCCGAA AAGCACTTTG CGACAGGGAT GTGGGAGTTA TGGCTGCCTC ATTACACATT 
TATCTCCAGA TGGTGAAGGA GAATGCATCT GGATACAAAG ACCTGACAAG CAGTTTCGTA 
GCTATTCTAA AGCAGGTGGT GGGAGGAAAG CTCCCCATAG ACTTCAATTA TCACAACGTG 
CCAGCACCTT GGCTACAAAT TCAGCTTTTA AGAATACTGG GGCTACTTGG AAAAGATGAT 
CCGGGAACCA GTGAATTAAT GTATGATGTT TTGGATGAGT CTTTAAGGAG AGCAGAGATA 
AATCACAACA TTACATATGC AATCTTGTTT GAATGTGTCC AGACAGTTTA TACAATATAC 
CCTAAATCCG ATCTACTTGA GAAGGCTGCC AAATGTATTG GAAAATTTGT CTTGTCTCCA 
AAAATTAACT TGAAGTACTT GGGTTTAAAG GCCCTAACTT ACGTTGTGCA GCAGGATCCG 
AACCTGGCGC TGCAGCACCA AATGACCATC ATCGAGTGCC TGGATCATCC TGACCCCATT 
ATTAAAAGAG AGACTTTGGA ACTTCTATAT AGGATCACCA ATGGGCAGAA TGTCGCCGTC 
ATCGTTCAGA AGATGCTCGA CTACTTGACC CAAAGCAAGG AGGAATATAC CATCATCAAC 
ATAGTTGGCA AAATAGCAGA ACTAGCTGAA AAATATGCCC CTGACAACGA ATGGTTCATT 
CGCACTATGA ATGCAGTGTT TTCTGTAGGA GGGGATAAAA TGCACCCAGA CATCCCCAAT 
AGCTTCTTGA GACTCCTTGC CGAAGGATTC GAGGAGGAAC AGGAAGATAG CCAGCTACGG 
CTTCATGCTG TCCAGTCTTA CCTTGTGTTG CTACAGAATG AGAAGGCGGT TTACCCCCAG 
AAATTTCTTC AAGTGATGAG TTGGGTGCTG GGAGAATATT CTTGCCTTAT GAAAAATGTT 
GATCCGGAGG TTGTCTTGGC AAATTTGCAC AGGATACTTA AGAGTAATGC TACCTCTGAA 
ACCAAAGCCT GGATTATGGC TGCCATCACT AAAATAACTT CTCGTACAAC ATGTTCTAAA 
ACAGTTGATA AACTGATAAC GGACTTCAGT ACCTCCTTGG ACACCTGCAT GAGGCAATAT 
GCTTTTGAGC TGAAAAACTT ATGTGAGGAC AGAGAATCAA TGAGAAAGTT GTTTCCAGTG 
GACTCTAGCT GTGAGGATAT TGTGGTAGAT GCTTCCTTGT CTTTCCTAGA TGGGTTTGTA 
GCTGAAGGAC TTGGCCGGGG TGCAGCACCA TATAAACCTC ATCACCAAAG ACAGGAGGAG 
CAGCTGTCTC AGGAAAAAGC TCTGAATTTT GAACCTTATG GATTGTCGCT TTCCCAGAGC 
TTCTCAACAT CTGGCTTTCC TGGCAGACAG TCTCCAACTG GACTTTCTCT GGGTTCTGAC 
ATTTCTGGTA ACAGTGCTGA GGTGGGACAG AAGGAGACCA ACAGTCTGAA GCTTGAAGGT 
GTGAAGAAGC TATGGGGAAA AGAAGGCTAC CTCCCCAAAA AGGAAAGCCT GGTGGAAAAA 
GAAGATAGCG TAAAGCACAC CGCAGAGAGC AGCACATCTG TGGAGCGTAC AGTCGGTAAC 
TTCGCCTTGC AGTCTGAGCA AGAGATTACC AGCCTCTCCA AGGAGGAGAA GGAGAAGCAG 
CAACTTGCCT CAACGCTCTT TGTTGGCCTG GGACTCCAGG GCAACGTCAG CCTGATGGGC 
AAAGGAGACT CTACCACCCA GAAGTTCAAA AGGAAATCTA AAACGGGCGA ATCCCAAAAT 
AGTGAGGAGT CATTCAGAAT CAGAAACACT GCTAGCCCGC CCCTCAGTCC TTCGCCCGAT 
GCATTACCTT GGAACAAAAA GCCCTTCCAA AAGAATGGGC ACCCAAGATT ACGGAAAGCT 
TCGCTGAATT CCTCTGAAGC TCCAGAACTG AAGGTCGTAT TGGATCGGTC TCATTCTCTG 
CCAGAAGCTG GACTGGGTGA GAAGCTTCTG GGTTGCAGAT TCTCGCCTTT ATCTTTGTTT 
GCGGATGGGA ACATGGAGAT TTTCCAGCCT TCTCCGAACA CTCATCCTGT AGTCAAAAAG 
CAACTCTCTT TGACGCCTTT CTTACCACAA GAAGTAGCCA AATACCCTCA TTCGGAGATT 
TTAGAACTTT GTGGCAACGA AACCGTCACG TTGTACTTGT GTAAAATATG GAAAGAGGAC 
AGCTTCATAC TTTTTGTATT TCTTGCCAAT AAAAGTCTCT CTACACTTAA AGATGTACAG 
CTGGAATTTG AACACTCCAA ACATTTTAAG ATCTCTGAAA ATCTTGGCTG CCATTTTGCT 
GAAATAGAAG CTCAGAGCAT AGAGAGCACC CAGAAGCGTG TAGCCACGGA GGAGCTCTGC 
TCAGAGGGAA TGCTGTCTGG ATCCCTCAGT TACCATCTAG AACTGGAGAC GTGTCTCAGG 
TTTTCAGTAA CTTTAGTGCT GTTGGATTTT ATCAGGCCAA TGAAAATGAC CACTGAAGAA 
TTTGGAAAAC TGTGGCTCTC CTTTTCTAAT GATGTCAAAC AAAACCTAAA ACTGACATCA 
TCTCAGGAAT CTTTATCAGT CATTTTAAAC ACCTTGCAGC AAAAACTGAA GCTACATGTT 
GTGGATATCA TAGGAAATGA GGGAATAGCA GCTTGCAGGC TGCTTCCGTC GGTCCCTGGC 
TTACTGCATT GCCGTATCCA CTCTGGGATG CTGGTCCTGT GGTTCAGGTC CCCATGTTCT 
GCTCTTCCAG ACTGTTTACT TTACCAGTGT CAGAAGGTGA TGGGAGAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015280962.1
CDS	1..3474
Translation	MYTSAKIHLGMMTERFKCRLHRGKICGGGGGAAGGGGGPGRGSATSRLGGLIRSITALTSKHEEEKLIQQELTSLKATVSAPTTTLRLMKECMVRLIYCEMLGYEASFGYIHAIKLAQQGNLFEKRVGYLAVSLFLHENHELLLLLVNTVVKDLQSTNLMEVCMALTVASQIFPREMIPAnullLPLVEDKLQHSKEIIRRKAVQALYKFYLIAPNQVQHIHNKFRKALCDRDVGVMAASLHIYLQMVKENASGYKDLTSSFVAILKQVVGGKLPIDFNYHNVPAPWLQIQLLRILGLLGKDDPGTSELMYDVLDESLRRAEINHNITYAILFECVQTVYTIYPKSDLLEKAAKCIGKFVLSPKINLKYLGLKALTYVVQQDPNLALQHQMTIIECLDHPDPIIKRETLELLYRITNGQNVAVIVQKMLDYLTQSKEEYTIINIVGKIAELAEKYAPDNEWFIRTMNAVFSVGGDKMHPDIPNSFLRLLAEGFEEEQEDSQLRLHAVQSYLVLLQNEKAVYPQKFLQVMSWVLGEYSCLMKNVDPEVVLANLHRILKSNATSETKAWIMAAITKITSRTTCSKTVDKLITDFSTSLDTCMRQYAFELKNLCEDRESMRKLFPVDSSCEDIVVDASLSFLDGFVAEGLGRGAAPYKPHHQRQEEQLSQEKALNFEPYGLSLSQSFSTSGFPGRQSPTGLSLGSDISGNSAEVGQKETNSLKLEGVKKLWGKEGYLPKKESLVEKEDSVKHTAESSTSVERTVGNFALQSEQEITSLSKEEKEKQQLASTLFVGLGLQGNVSLMGKGDSTTQKFKRKSKTGESQNSEESFRIRNTASPPLSPSPDALPWNKKPFQKNGHPRLRKASLNSSEAPELKVVLDRSHSLPEAGLGEKLLGCRFSPLSLFADGNMEIFQPSPNTHPVVKKQLSLTPFLPQEVAKYPHSEILELCGNETVTLYLCKIWKEDSFILFVFLANKSLSTLKDVQLEFEHSKHFKISENLGCHFAEIEAQSIESTQKRVATEELCSEGMLSGSLSYHLELETCLRFSVTLVLLDFIRPMKMTTEEFGKLWLSFSNDVKQNLKLTSSQESLSVILNTLQQKLKLHVVDIIGNEGIAACRLLPSVPGLLHCRIHSGMLVLWFRSPCSALPDCLLYQCQKVMGES

Target ORF information:

RefSeq Version	XM_015425476.1
Organism	Gekko japonicus
Definition	Gekko japonicus adaptor related protein complex 4 epsilon 1 subunit (AP4E1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015425476.1

ORF Insert Sequence:

ATGTATACAA GTGCTAAAAT CCACCTTGGC ATGATGACAG AGAGGTTTAA ATGCAGACTG 
CACCGAGGGA AGATCTGTGG AGGCGGCGGC GGCGCGGCAG GAGGAGGAGG AGGGCCCGGC 
AGGGGCTCGG CCACCTCGCG GCTCGGCGGG CTCATCCGGA GCATCACCGC GCTCACCTCC 
AAGCATGAGG AAGAAAAATT AATCCAACAG GAATTAACTA GCTTGAAAGC AACAGTTTCT 
GCACCCACTA CAACCCTGAG GCTGATGAAG GAATGCATGG TGAGGCTTAT TTATTGTGAG 
ATGCTGGGCT ATGAGGCCTC GTTTGGATAC ATCCACGCTA TCAAGCTGGC CCAACAAGGA 
AACCTTTTTG AAAAAAGAGT CGGCTACTTG GCAGTTTCTT TGTTTCTTCA TGAGAATCAC 
GAGCTGTTGC TTTTGCTTGT GAACACCGTA GTGAAGGATT TGCAGAGCAC TAACCTCATG 
GAAGTATGCA TGGCTCTTAC TGTGGCAAGT CAGATATTTC CACGGGAAAT GATTCCTGCT 
NAACTTCCGC TAGTGGAAGA CAAGCTCCAG CATTCAAAGG AAATCATTAG AAGGAAAGCA 
GTTCAAGCAC TGTACAAGTT CTACCTTATT GCTCCCAACC AAGTCCAGCA CATCCATAAC 
AAATTCCGAA AAGCACTTTG CGACAGGGAT GTGGGAGTTA TGGCTGCCTC ATTACACATT 
TATCTCCAGA TGGTGAAGGA GAATGCATCT GGATACAAAG ACCTGACAAG CAGTTTCGTA 
GCTATTCTAA AGCAGGTGGT GGGAGGAAAG CTCCCCATAG ACTTCAATTA TCACAACGTG 
CCAGCACCTT GGCTACAAAT TCAGCTTTTA AGAATACTGG GGCTACTTGG AAAAGATGAT 
CCGGGAACCA GTGAATTAAT GTATGATGTT TTGGATGAGT CTTTAAGGAG AGCAGAGATA 
AATCACAACA TTACATATGC AATCTTGTTT GAATGTGTCC AGACAGTTTA TACAATATAC 
CCTAAATCCG ATCTACTTGA GAAGGCTGCC AAATGTATTG GAAAATTTGT CTTGTCTCCA 
AAAATTAACT TGAAGTACTT GGGTTTAAAG GCCCTAACTT ACGTTGTGCA GCAGGATCCG 
AACCTGGCGC TGCAGCACCA AATGACCATC ATCGAGTGCC TGGATCATCC TGACCCCATT 
ATTAAAAGAG AGACTTTGGA ACTTCTATAT AGGATCACCA ATGGGCAGAA TGTCGCCGTC 
ATCGTTCAGA AGATGCTCGA CTACTTGACC CAAAGCAAGG AGGAATATAC CATCATCAAC 
ATAGTTGGCA AAATAGCAGA ACTAGCTGAA AAATATGCCC CTGACAACGA ATGGTTCATT 
CGCACTATGA ATGCAGTGTT TTCTGTAGGA GGGGATAAAA TGCACCCAGA CATCCCCAAT 
AGCTTCTTGA GACTCCTTGC CGAAGGATTC GAGGAGGAAC AGGAAGATAG CCAGCTACGG 
CTTCATGCTG TCCAGTCTTA CCTTGTGTTG CTACAGAATG AGAAGGCGGT TTACCCCCAG 
AAATTTCTTC AAGTGATGAG TTGGGTGCTG GGAGAATATT CTTGCCTTAT GAAAAATGTT 
GATCCGGAGG TTGTCTTGGC AAATTTGCAC AGGATACTTA AGAGTAATGC TACCTCTGAA 
ACCAAAGCCT GGATTATGGC TGCCATCACT AAAATAACTT CTCGTACAAC ATGTTCTAAA 
ACAGTTGATA AACTGATAAC GGACTTCAGT ACCTCCTTGG ACACCTGCAT GAGGCAATAT 
GCTTTTGAGC TGAAAAACTT ATGTGAGGAC AGAGAATCAA TGAGAAAGTT GTTTCCAGTG 
GACTCTAGCT GTGAGGATAT TGTGGTAGAT GCTTCCTTGT CTTTCCTAGA TGGGTTTGTA 
GCTGAAGGAC TTGGCCGGGG TGCAGCACCA TATAAACCTC ATCACCAAAG ACAGGAGGAG 
CAGCTGTCTC AGGAAAAAGC TCTGAATTTT GAACCTTATG GATTGTCGCT TTCCCAGAGC 
TTCTCAACAT CTGGCTTTCC TGGCAGACAG TCTCCAACTG GACTTTCTCT GGGTTCTGAC 
ATTTCTGGTA ACAGTGCTGA GGTGGGACAG AAGGAGACCA ACAGTCTGAA GCTTGAAGGT 
GTGAAGAAGC TATGGGGAAA AGAAGGCTAC CTCCCCAAAA AGGAAAGCCT GGTGGAAAAA 
GAAGATAGCG TAAAGCACAC CGCAGAGAGC AGCACATCTG TGGAGCGTAC AGTCGGTAAC 
TTCGCCTTGC AGTCTGAGCA AGAGATTACC AGCCTCTCCA AGGAGGAGAA GGAGAAGCAG 
CAACTTGCCT CAACGCTCTT TGTTGGCCTG GGACTCCAGG GCAACGTCAG CCTGATGGGC 
AAAGGAGACT CTACCACCCA GAAGTTCAAA AGGAAATCTA AAACGGGCGA ATCCCAAAAT 
AGTGAGGAGT CATTCAGAAT CAGAAACACT GCTAGCCCGC CCCTCAGTCC TTCGCCCGAT 
GCATTACCTT GGAACAAAAA GCCCTTCCAA AAGAATGGGC ACCCAAGATT ACGGAAAGCT 
TCGCTGAATT CCTCTGAAGC TCCAGAACTG AAGGTCGTAT TGGATCGGTC TCATTCTCTG 
CCAGAAGCTG GACTGGGTGA GAAGCTTCTG GGTTGCAGAT TCTCGCCTTT ATCTTTGTTT 
GCGGATGGGA ACATGGAGAT TTTCCAGCCT TCTCCGAACA CTCATCCTGT AGTCAAAAAG 
CAACTCTCTT TGACGCCTTT CTTACCACAA GAAGTAGCCA AATACCCTCA TTCGGAGATT 
TTAGAACTTT GTGGCAACGA AACCGTCACG TTGTACTTGT GTAAAATATG GAAAGAGGAC 
AGCTTCATAC TTTTTGTATT TCTTGCCAAT AAAAGTCTCT CTACACTTAA AGATGTACAG 
CTGGAATTTG AACACTCCAA ACATTTTAAG ATCTCTGAAA ATCTTGGCTG CCATTTTGCT 
GAAATAGAAG CTCAGAGCAT AGAGAGCACC CAGAAGCGTG TAGCCACGGA GGAGCTCTGC 
TCAGAGGGAA TGCTGTCTGG ATCCCTCAGT TACCATCTAG AACTGGAGAC GTGTCTCAGG 
TTTTCAGTAA CTTTAGTGCT GTTGGATTTT ATCAGGCCAA TGAAAATGAC CACTGAAGAA 
TTTGGAAAAC TGTGGCTCTC CTTTTCTAAT GATGTCAAAC AAAACCTAAA ACTGACATCA 
TCTCAGGAAT CTTTATCAGT CATTTTAAAC ACCTTGCAGC AAAAACTGAA GCTACATGTT 
GTGGATATCA TAGGAAATGA GGGAATAGCA GCTTGCAGGC TGCTTCCGTC GGTCCCTGGC 
TTACTGCATT GCCGTATCCA CTCTGGGATG CTGGTCCTGT GGTTCAGGTC CCCATGTTCT 
GCTCTTCCAG ACTGTTTACT TTACCAGTGT CAGAAGGTGA TGGGAGAATC CTGA