WFS1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	WFS1
Entrez Gene ID	107121379
Full Name	wolframin ER transmembrane glycoprotein
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015424293.1	XP_015279779.1	wolframin

Pathways from BioSystems
Gene Ontology

The following WFS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WFS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe32612	XM_015424293.1 Latest version!	Gekko japonicus Wolfram syndrome 1 (wolframin) (WFS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WFS1 ( XM_015424293.1 ) cDNA ORF clone, Gekko japonicus -> XP_015279779.1 Gekko japonicus Wolfram syndrome 1 (wolframin) (WFS1), mRNA.

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CloneID	OGe32612
Clone ID Related Accession (Same CDS sequence)	XM_015424293.1
Accession Version	XM_015424293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	wolframin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015174342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAAGCC AAGACGCCAC AAAGGAGGAA CCAGAGGCAC TCTTTGAGGA ACTGCTTGAA 
AGGGCAAAAG CTGGGGAATC CAAAGCCCAG GCAGAGGTGG GGAAGTACTA CCTGAAACTG 
GCTGAAGACC GAGATGAGGA GCTGAACAGT TGCAGAGCAG TTCAGTGGCT GGTCCTGGCC 
GCTAAACAAG GGCGGAGGGG AGCCAGCAAA CTGCTGAAGC AATGCCTAGC GGAGCGGAGA 
GCTGGCGCGG TCATGTACCG GAAACTAAAT CCAGACAAGA AAAAGCAGTT GGCTGTTTCA 
GAACTATTAG AAAATGTTGG CCAAGTTGAT GGTGAAGGTG GTGAGAAGCA GCCTGGCCCA 
GTTCCCAAGT CTGTTCAGAA GCAAAGAAGA ATGCTGGAGC GGCTGGTGAG CAGTGAATCA 
AAGAGTTACA TTGCCTTGGA TGGTTTTGTT GAAATAACCA AGAAGTACGC CAGAGGCATC 
ATCCCAACCA AGCTCCTGGA TCAAGATGAG GACGATGATG AGCTGGCCGG CAAGAGCCCA 
GAGGAGCTTC CGCTCCGGCT CAGGGTTATC AAGTACCCCC TTCATGCCAT CATGGAGCTG 
AAGGAGCACC TCATCGACAT TGCCTCTAAA GCAGGGGTGC ACTGGCTCTC CACAATCGTT 
CCCACACACC ACATCAATGC CCTCATCTTC TTCTTCATCA TCAGCAACCT GACGATTGAC 
TTTTTTGCCT TTTTTATCCC GCTAGTCATC TTCTATCTCT CCTTCATTTC CATGGTGATT 
TGCACCCTGA AAGTCTTTCA AGATAGTAAA GCATGGGAGA ACTTCCGCAC CCTCACGGAT 
TTGCTGCTTC GTTTTGAGCC AAATCTAGAT GTTGAGGAAG CAGAAGTGAA CTTTGGCTGG 
AACCACTTAG AGCCATACCT CCACTTCCTC CTGTCAGTCT TCTTTGTCAT TTTCTCCTTC 
CCTATAGCAA GCAAGGACTG GATACCCTGC TCTGAGTTGG CAGTCATCTC CACTTTCTTC 
ATGGGGATGA GCTACATGAG TTTAGCCACG AGTGTGGAGT CTTACACCCG AAGAGCGCTG 
CTGACAGAGG TGGCTGCCGG GGCCCTCTCC TTGCTGCAGG TGGTGCCTCT GGATCTGGGC 
CCACTCAAAC TACTGGGGAA AACCTTTTAC AGTTTCCCTG TTGGCCACTT CTTCGTGTTC 
AACGTGAGCA TCCCTTGTCT GCTGTTTCTG TATTTGTTTT ATCTGTTCTT TAGAATGGCA 
AAGATGCGGA ACTTCAAGGG CACATATTAC TACTTGGTTC CGTACATGGT GTGTTTCATG 
TGGTGTGAAC TCTCGGTGGT TATTTTGCAG CACTCAGCTG GCATTGGACT GATCCGTGCT 
TCTGTGGGTT ACTTTTTGTT CCTTTTTGCG CTTCCGATCC TACTGTTAGG CGTTGCTCTG 
ATGTGTCTCT TCCATTTTTT AAAGTGGTTT GTAACACTGG ACCTTGTGAA GATTGTCATA 
ACCCTTGTGT TGTGTGCTGT TCCTTTGCTT ATCCGGTGGT GGACGAAGAC CAGCTTCTCT 
GTGGCCGAGA TGGTGAAGTC CCTAACAAAG AGTTCAATTG TAAAACTCAT TCTGGTATGG 
ATTACTGCCA TAGTTCTGTT CTGTTGGTTT TATGTCTACC GCTCAGAGGG AATGAAAGTC 
TATAACTCTA CCTTGACATG GAATCAGTAT GGGTTTTTGT GTGGTCCACG GGCTTGGAAG 
GAAACCAACA TGGCCCGGAC CCAGATGTTA TGCAGTCATC TGGAGGGCCA CAGAGTGACG 
TGGACTGGAA GGTTCAAATA TGTCCGAATC ACTGACATTG ACAATAGTGC GGAATCGGCA 
ATAAACATGC TTCCCTTTTT TATTGGAGAT TGGCTGAGGT GCTTGTATGG TGAAACCTAT 
CCGGTGTGTG CCCCTGAAAA CATCACTGTC GAAGAGGAGG AGCTGTGCCG CCTGAAATAT 
CTGACCAAAC ACAACTGCCA CATGAAACGG TTCGACCGTT ACAAATTTGA AATATCTGTG 
GGCATGCCTC TCAGTAGCAT GAATGGAAAT AAGATGAAAG AGGAGGAGGA TGCAACCAAA 
GACATTGTGC TGAAGGCAAG CAACGAGTTC AAGAAGGTGC TGTTGAACCT GAGGCAGGGA 
AGCATCATCG AGTTCAGCAC CATCCTGGAA GGCCGTTTGG GCAGCAAGTG GCCAGTCTTT 
GAACTGAAGG CCATCTCGTG CTTGAACTGC ATGGCTGAAC TCCTGCCTGC AGGGAGGCAT 
GTGAAAGTCG AGCATGACTG GAGAAGCACT GTTCAGAAAG CACTCAAGTT TGCCTTTGAT 
TTTTTCTTTT ACCCATTTTT GTCAGTTGCA TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015279779.1
CDS	72..2447
Translation	MESQDATKEEPEALFEELLERAKAGESKAQAEVGKYYLKLAEDRDEELNSCRAVQWLVLAAKQGRRGASKLLKQCLAERRAGAVMYRKLNPDKKKQLAVSELLENVGQVDGEGGEKQPGPVPKSVQKQRRMLERLVSSESKSYIALDGFVEITKKYARGIIPTKLLDQDEDDDELAGKSPEELPLRLRVIKYPLHAIMELKEHLIDIASKAGVHWLSTIVPTHHINALIFFFIISNLTIDFFAFFIPLVIFYLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEEAEVNFGWNHLEPYLHFLLSVFFVIFSFPIASKDWIPCSELAVISTFFMGMSYMSLATSVESYTRRALLTEVAAGALSLLQVVPLDLGPLKLLGKTFYSFPVGHFFVFNVSIPCLLFLYLFYLFFRMAKMRNFKGTYYYLVPYMVCFMWCELSVVILQHSAGIGLIRASVGYFLFLFALPILLLGVALMCLFHFLKWFVTLDLVKIVITLVLCAVPLLIRWWTKTSFSVAEMVKSLTKSSIVKLILVWITAIVLFCWFYVYRSEGMKVYNSTLTWNQYGFLCGPRAWKETNMARTQMLCSHLEGHRVTWTGRFKYVRITDIDNSAESAINMLPFFIGDWLRCLYGETYPVCAPENITVEEEELCRLKYLTKHNCHMKRFDRYKFEISVGMPLSSMNGNKMKEEEDATKDIVLKASNEFKKVLLNLRQGSIIEFSTILEGRLGSKWPVFELKAISCLNCMAELLPAGRHVKVEHDWRSTVQKALKFAFDFFFYPFLSVAY

Target ORF information:

RefSeq Version	XM_015424293.1
Organism	Gekko japonicus
Definition	Gekko japonicus Wolfram syndrome 1 (wolframin) (WFS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015424293.1

ORF Insert Sequence:

ATGGAAAGCC AAGACGCCAC AAAGGAGGAA CCAGAGGCAC TCTTTGAGGA ACTGCTTGAA 
AGGGCAAAAG CTGGGGAATC CAAAGCCCAG GCAGAGGTGG GGAAGTACTA CCTGAAACTG 
GCTGAAGACC GAGATGAGGA GCTGAACAGT TGCAGAGCAG TTCAGTGGCT GGTCCTGGCC 
GCTAAACAAG GGCGGAGGGG AGCCAGCAAA CTGCTGAAGC AATGCCTAGC GGAGCGGAGA 
GCTGGCGCGG TCATGTACCG GAAACTAAAT CCAGACAAGA AAAAGCAGTT GGCTGTTTCA 
GAACTATTAG AAAATGTTGG CCAAGTTGAT GGTGAAGGTG GTGAGAAGCA GCCTGGCCCA 
GTTCCCAAGT CTGTTCAGAA GCAAAGAAGA ATGCTGGAGC GGCTGGTGAG CAGTGAATCA 
AAGAGTTACA TTGCCTTGGA TGGTTTTGTT GAAATAACCA AGAAGTACGC CAGAGGCATC 
ATCCCAACCA AGCTCCTGGA TCAAGATGAG GACGATGATG AGCTGGCCGG CAAGAGCCCA 
GAGGAGCTTC CGCTCCGGCT CAGGGTTATC AAGTACCCCC TTCATGCCAT CATGGAGCTG 
AAGGAGCACC TCATCGACAT TGCCTCTAAA GCAGGGGTGC ACTGGCTCTC CACAATCGTT 
CCCACACACC ACATCAATGC CCTCATCTTC TTCTTCATCA TCAGCAACCT GACGATTGAC 
TTTTTTGCCT TTTTTATCCC GCTAGTCATC TTCTATCTCT CCTTCATTTC CATGGTGATT 
TGCACCCTGA AAGTCTTTCA AGATAGTAAA GCATGGGAGA ACTTCCGCAC CCTCACGGAT 
TTGCTGCTTC GTTTTGAGCC AAATCTAGAT GTTGAGGAAG CAGAAGTGAA CTTTGGCTGG 
AACCACTTAG AGCCATACCT CCACTTCCTC CTGTCAGTCT TCTTTGTCAT TTTCTCCTTC 
CCTATAGCAA GCAAGGACTG GATACCCTGC TCTGAGTTGG CAGTCATCTC CACTTTCTTC 
ATGGGGATGA GCTACATGAG TTTAGCCACG AGTGTGGAGT CTTACACCCG AAGAGCGCTG 
CTGACAGAGG TGGCTGCCGG GGCCCTCTCC TTGCTGCAGG TGGTGCCTCT GGATCTGGGC 
CCACTCAAAC TACTGGGGAA AACCTTTTAC AGTTTCCCTG TTGGCCACTT CTTCGTGTTC 
AACGTGAGCA TCCCTTGTCT GCTGTTTCTG TATTTGTTTT ATCTGTTCTT TAGAATGGCA 
AAGATGCGGA ACTTCAAGGG CACATATTAC TACTTGGTTC CGTACATGGT GTGTTTCATG 
TGGTGTGAAC TCTCGGTGGT TATTTTGCAG CACTCAGCTG GCATTGGACT GATCCGTGCT 
TCTGTGGGTT ACTTTTTGTT CCTTTTTGCG CTTCCGATCC TACTGTTAGG CGTTGCTCTG 
ATGTGTCTCT TCCATTTTTT AAAGTGGTTT GTAACACTGG ACCTTGTGAA GATTGTCATA 
ACCCTTGTGT TGTGTGCTGT TCCTTTGCTT ATCCGGTGGT GGACGAAGAC CAGCTTCTCT 
GTGGCCGAGA TGGTGAAGTC CCTAACAAAG AGTTCAATTG TAAAACTCAT TCTGGTATGG 
ATTACTGCCA TAGTTCTGTT CTGTTGGTTT TATGTCTACC GCTCAGAGGG AATGAAAGTC 
TATAACTCTA CCTTGACATG GAATCAGTAT GGGTTTTTGT GTGGTCCACG GGCTTGGAAG 
GAAACCAACA TGGCCCGGAC CCAGATGTTA TGCAGTCATC TGGAGGGCCA CAGAGTGACG 
TGGACTGGAA GGTTCAAATA TGTCCGAATC ACTGACATTG ACAATAGTGC GGAATCGGCA 
ATAAACATGC TTCCCTTTTT TATTGGAGAT TGGCTGAGGT GCTTGTATGG TGAAACCTAT 
CCGGTGTGTG CCCCTGAAAA CATCACTGTC GAAGAGGAGG AGCTGTGCCG CCTGAAATAT 
CTGACCAAAC ACAACTGCCA CATGAAACGG TTCGACCGTT ACAAATTTGA AATATCTGTG 
GGCATGCCTC TCAGTAGCAT GAATGGAAAT AAGATGAAAG AGGAGGAGGA TGCAACCAAA 
GACATTGTGC TGAAGGCAAG CAACGAGTTC AAGAAGGTGC TGTTGAACCT GAGGCAGGGA 
AGCATCATCG AGTTCAGCAC CATCCTGGAA GGCCGTTTGG GCAGCAAGTG GCCAGTCTTT 
GAACTGAAGG CCATCTCGTG CTTGAACTGC ATGGCTGAAC TCCTGCCTGC AGGGAGGCAT 
GTGAAAGTCG AGCATGACTG GAGAAGCACT GTTCAGAAAG CACTCAAGTT TGCCTTTGAT 
TTTTTCTTTT ACCCATTTTT GTCAGTTGCA TATTGA