CRIM1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	107120653
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015423434.1	XP_015278920.1	cysteine-rich motor neuron 1 protein isoform X1
XM_015423500.1	XP_015278986.1	cysteine-rich motor neuron 1 protein isoform X2

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe15574	XM_015423434.1 Latest version!	Gekko japonicus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGe15575	XM_015423500.1 Latest version!	Gekko japonicus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_015423434.1 ) cDNA ORF clone, Gekko japonicus -> XP_015278920.1 Gekko japonicus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGe15574
Clone ID Related Accession (Same CDS sequence)	XM_015423434.1
Accession Version	XM_015423434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015159725.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGG CAACGCTGTC GGCGGGGCGG AGGCGCCGGG GCAGCGGCGG CGACTGGCAC 
CTGGCGGCGG GGCGGCTCCT GTTCCTGGCG CTGCTGCTCT TGGGGGAGTC CGGGACGCGG 
GCGTTCGTCT GCCTGCCGTG CGACGAGTCC AGGTGCGAGG AGCCCAAGAA CTGCCCCGGC 
GGCGTCGTCC TGGACATCTG CGGCTGCTGC TTCATGTGTG CCCGGCAACG CAACGAGAGT 
TGTGGGGGCT TCTTTGGGCT CCACGGAGCC TGCGACAAGG GGCTGCGCTG CGTGATCCGA 
CCCCCGCTCA ACGGAGACTC CCTCACCGAA TACGAAGCGG GCGTCTGTGA AGATGAAAAC 
TGGGATGATC AGCTACTTGG ATTTGAGCCT TGCAATGAAA ACCTGATAAC AGGCTGCAAT 
ATAATCAATG GAAAATGCGA ATGTGATACT ATTAGGACCT GTAGTAATCC CTTTGAGTTT 
CCAAGCCGGG ATACTTGTCT ATCAGCTTTA AAAAAGATTG AAGAAGAAAA GCCTGACTGT 
TCCAAGGCCC GTTGTGAAGT ACAGTTTTCT CCTCGCTGTC CAGAAGATTC CATTTTGATT 
GAAGGCTATG CTCCACCTGG TGAATGCTGT CCTCTCCCAA GCCGCTGTGT GTGCAATCCT 
ACGGGGTGTT TGAGGAAAGT TTGCCAGCCT GGCTATTTGA ATATATTGGT TTCTAAAGCA 
TCAGGCAAAC CAGGGGAATG CTGTGACTTT TATGAATGTA AACCAGTTTT CAGCGTAGAC 
TGCAGTACAG TAGAGTGTCC TCCTGTCCAG CAAGTTGTTT GCCCCTTGGA TAGCTATGAA 
ACCCAAGTCA GGCTGACAGC TGATGGATGT TGTACTCTTC CTACAAGGTG CGAGTGTCTT 
TCTGGTTTAT GTGGTATTCC AAAATGTGAG GCAGGCTACA TCCCCCAAAT AGTGTCACGT 
GGAGACGGAA CGCCTGGCAA GTGCTGTGAT GTTTTTGAAT GCGTCAGTGA AGTGAAGCCA 
GCCTGTATAT ATAATAGTGT GGAATATTAT GATGGAGATA TGTTTCGAAT GGATGCTTGC 
CGTTTTTGTC GGTGTCAAGG TGGCGTCTCT ATTTGTTTCT CCGCACAGTG TGGGGAGCTA 
CATTGTGAGA GATACTATGT ACCAGAAGGA GAGTGCTGTC CAGTATGCGA AGATTCGGTT 
TCTCCAGTTA GTAACCCGGC TGGCTGTTAT GCTGATGGCC AGATCCAAGC ACATGGTGAT 
CGCTGGAGGG AAGATGATTG TACTTTCTGC CAGTGTATCA ATGGAGAGTC CCATTGTGTT 
GCCACAGCCT GTGGACAGAA CTGCTTGAAT CCTGTAAAAG TTCCTGGGGA ATGCTGCCCA 
ATGTGTGAAG AACCAACTTA TATCACAATA GGTCCTCCCA CATGTTATCC TCTCGTGAAC 
TGCACGTTAA CTGAGAGAGA CTGTGTCTAC AACTTCAGAG TTGACCAGAA TGGTTGTCGC 
ATCTGTCAAT GCAAAACAAG AGAGGAGCTG TGTATTGACC TCATGGAAGG TTGTTCCTTG 
GTTTGTCCCT TCGGTTTCCA GACTGATGAC CGCAACTGTA AGATCTGTCA GTGCCGACCA 
CGACCTAAGA AATGCAAGCC CATAGTATGT GACAAGTACT GTCCATCTGG ATACCTGAAG 
AACAAACATG GATGTGAAAT CTGTCGATGT AAGAAATGCC CAGAACAGTC ATGCGACAAG 
ATCTGCCCTA ATGGCTTCCA GCAGGATAAT GAAGGCTGTC TTATCTGCAA ATGTAGAGAG 
TTACCAGCTT CTGTAATGCC ACCAGCTAAG ACTGGTTCTT GCCTGTCAGT GGATGGGCAC 
CGTCATGAAA ATGAAGAAAG TTGGCATGAT GGCTGCCGTG AATGTTACTG CCATAATGGA 
CGGGAAATGT GTGCTTTGAT CACCTGCTCT GTACCCAATT GTGGAAATCC CACCATACAT 
CCAGGCAAAT GTTGTCCATC ATGTCCAGAT GAATCTACTT TACAGAAGCC AGAACTCAGT 
GGTCCGACTA TCTGCTATGT TCCAGGTGGC GAGTACTTTG TGGAAGGAGA TACATGGAAC 
ATTGATTCTT GCACACAATG TACTTGTCAT AATGCACGAG TTTTGTGCGA GACTGAAGTC 
TGTCCCCCTC TTCTTTGTCG AAATCCCACC CGTTCGCAAG ATTCATGCTG TCCACAGTGC 
CCAGATGAGC CCGCTCAGCC TTCCTTGTCA AGCAACGAGA GCATGCCTAG CTACTGCAAA 
AATGATGAAG GTGATATTTT CCTGACTGCT GAATCCTGGA AACCAAATGT TTGTGCTAGC 
TGTGTTTGCA TGGATGGTAC TATCAGCTGC TACTCTGAGT CTTGCCCACC AGTGTCCTGT 
GACAGGCCTG TTCTGAGAAA GGGCCAGTGT TGCCCTTATT GTATAGAAGA CACAACATCA 
AAGAAAGCAG TTTGTCACTT TAATGGGAAA ACATATGCAG ATGAAGAACG TTGGGACATT 
GACAGCTGCA CACACTGCTA CTGCCTGCAG GGTCAGACCC TTTGTTCCAC TGTCAGCTGC 
CCTCCTCTTC CTTGTGTGGA ACCCATCAAT GTTGAAGGAA GTTGCTGCCC CATGTGCCCA 
GAGATGTATG TACCTGAACC TACCAACATT CCAATTGAGA AGACAAACCA TCGAGAATAT 
AATGAACTGG AAGGACCAGT TTGGCCTACG CTCAATGAAA ATGACATTGT TCGTATCCAT 
AGAGATATGG GTCACCTTCA GGGGGATTTT AGAGAAAACA GAGGATCAGA ATCAAGCACA 
GAGGAATCTC TTAGCTCTCT TGCTTGGGTG ACGGTGCCCA TCATGACAGC CCTTGTTCTT 
ATCATAGCAC TTCTACTTGT CAATCAGAAG AAACAGTGGA TACCAGTATC CTGCTACAGA 
GCCCCATCAA AGCCTTCCTG CCTAAACAAT CAACTGGTAT ATGTGGACTG CAAGAAAGGA 
ACTATGGTTC AGGTAGACAG TTCTCAGAGG ATGCTAAGAA TTGCTGACCC AGATTCAAGA 
TACAGTGGAT TCTACAGCAT GCAAAAACAG AACAACTTAC AGGCAGATAA TTTCTATCAA 
ACAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015278920.1
CDS	42..3170
Translation	MYLATLSAGRRRRGSGGDWHLAAGRLLFLALLLLGESGTRAFVCLPCDESRCEEPKNCPGGVVLDICGCCFMCARQRNESCGGFFGLHGACDKGLRCVIRPPLNGDSLTEYEAGVCEDENWDDQLLGFEPCNENLITGCNIINGKCECDTIRTCSNPFEFPSRDTCLSALKKIEEEKPDCSKARCEVQFSPRCPEDSILIEGYAPPGECCPLPSRCVCNPTGCLRKVCQPGYLNILVSKASGKPGECCDFYECKPVFSVDCSTVECPPVQQVVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGIPKCEAGYIPQIVSRGDGTPGKCCDVFECVSEVKPACIYNSVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCERYYVPEGECCPVCEDSVSPVSNPAGCYADGQIQAHGDRWREDDCTFCQCINGESHCVATACGQNCLNPVKVPGECCPMCEEPTYITIGPPTCYPLVNCTLTERDCVYNFRVDQNGCRICQCKTREELCIDLMEGCSLVCPFGFQTDDRNCKICQCRPRPKKCKPIVCDKYCPSGYLKNKHGCEICRCKKCPEQSCDKICPNGFQQDNEGCLICKCRELPASVMPPAKTGSCLSVDGHRHENEESWHDGCRECYCHNGREMCALITCSVPNCGNPTIHPGKCCPSCPDESTLQKPELSGPTICYVPGGEYFVEGDTWNIDSCTQCTCHNARVLCETEVCPPLLCRNPTRSQDSCCPQCPDEPAQPSLSSNESMPSYCKNDEGDIFLTAESWKPNVCASCVCMDGTISCYSESCPPVSCDRPVLRKGQCCPYCIEDTTSKKAVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREYNELEGPVWPTLNENDIVRIHRDMGHLQGDFRENRGSESSTEESLSSLAWVTVPIMTALVLIIALLLVNQKKQWIPVSCYRAPSKPSCLNNQLVYVDCKKGTMVQVDSSQRMLRIADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_015423434.1
Organism	Gekko japonicus
Definition	Gekko japonicus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015423434.1

ORF Insert Sequence:

ATGTACCTGG CAACGCTGTC GGCGGGGCGG AGGCGCCGGG GCAGCGGCGG CGACTGGCAC 
CTGGCGGCGG GGCGGCTCCT GTTCCTGGCG CTGCTGCTCT TGGGGGAGTC CGGGACGCGG 
GCGTTCGTCT GCCTGCCGTG CGACGAGTCC AGGTGCGAGG AGCCCAAGAA CTGCCCCGGC 
GGCGTCGTCC TGGACATCTG CGGCTGCTGC TTCATGTGTG CCCGGCAACG CAACGAGAGT 
TGTGGGGGCT TCTTTGGGCT CCACGGAGCC TGCGACAAGG GGCTGCGCTG CGTGATCCGA 
CCCCCGCTCA ACGGAGACTC CCTCACCGAA TACGAAGCGG GCGTCTGTGA AGATGAAAAC 
TGGGATGATC AGCTACTTGG ATTTGAGCCT TGCAATGAAA ACCTGATAAC AGGCTGCAAT 
ATAATCAATG GAAAATGCGA ATGTGATACT ATTAGGACCT GTAGTAATCC CTTTGAGTTT 
CCAAGCCGGG ATACTTGTCT ATCAGCTTTA AAAAAGATTG AAGAAGAAAA GCCTGACTGT 
TCCAAGGCCC GTTGTGAAGT ACAGTTTTCT CCTCGCTGTC CAGAAGATTC CATTTTGATT 
GAAGGCTATG CTCCACCTGG TGAATGCTGT CCTCTCCCAA GCCGCTGTGT GTGCAATCCT 
ACGGGGTGTT TGAGGAAAGT TTGCCAGCCT GGCTATTTGA ATATATTGGT TTCTAAAGCA 
TCAGGCAAAC CAGGGGAATG CTGTGACTTT TATGAATGTA AACCAGTTTT CAGCGTAGAC 
TGCAGTACAG TAGAGTGTCC TCCTGTCCAG CAAGTTGTTT GCCCCTTGGA TAGCTATGAA 
ACCCAAGTCA GGCTGACAGC TGATGGATGT TGTACTCTTC CTACAAGGTG CGAGTGTCTT 
TCTGGTTTAT GTGGTATTCC AAAATGTGAG GCAGGCTACA TCCCCCAAAT AGTGTCACGT 
GGAGACGGAA CGCCTGGCAA GTGCTGTGAT GTTTTTGAAT GCGTCAGTGA AGTGAAGCCA 
GCCTGTATAT ATAATAGTGT GGAATATTAT GATGGAGATA TGTTTCGAAT GGATGCTTGC 
CGTTTTTGTC GGTGTCAAGG TGGCGTCTCT ATTTGTTTCT CCGCACAGTG TGGGGAGCTA 
CATTGTGAGA GATACTATGT ACCAGAAGGA GAGTGCTGTC CAGTATGCGA AGATTCGGTT 
TCTCCAGTTA GTAACCCGGC TGGCTGTTAT GCTGATGGCC AGATCCAAGC ACATGGTGAT 
CGCTGGAGGG AAGATGATTG TACTTTCTGC CAGTGTATCA ATGGAGAGTC CCATTGTGTT 
GCCACAGCCT GTGGACAGAA CTGCTTGAAT CCTGTAAAAG TTCCTGGGGA ATGCTGCCCA 
ATGTGTGAAG AACCAACTTA TATCACAATA GGTCCTCCCA CATGTTATCC TCTCGTGAAC 
TGCACGTTAA CTGAGAGAGA CTGTGTCTAC AACTTCAGAG TTGACCAGAA TGGTTGTCGC 
ATCTGTCAAT GCAAAACAAG AGAGGAGCTG TGTATTGACC TCATGGAAGG TTGTTCCTTG 
GTTTGTCCCT TCGGTTTCCA GACTGATGAC CGCAACTGTA AGATCTGTCA GTGCCGACCA 
CGACCTAAGA AATGCAAGCC CATAGTATGT GACAAGTACT GTCCATCTGG ATACCTGAAG 
AACAAACATG GATGTGAAAT CTGTCGATGT AAGAAATGCC CAGAACAGTC ATGCGACAAG 
ATCTGCCCTA ATGGCTTCCA GCAGGATAAT GAAGGCTGTC TTATCTGCAA ATGTAGAGAG 
TTACCAGCTT CTGTAATGCC ACCAGCTAAG ACTGGTTCTT GCCTGTCAGT GGATGGGCAC 
CGTCATGAAA ATGAAGAAAG TTGGCATGAT GGCTGCCGTG AATGTTACTG CCATAATGGA 
CGGGAAATGT GTGCTTTGAT CACCTGCTCT GTACCCAATT GTGGAAATCC CACCATACAT 
CCAGGCAAAT GTTGTCCATC ATGTCCAGAT GAATCTACTT TACAGAAGCC AGAACTCAGT 
GGTCCGACTA TCTGCTATGT TCCAGGTGGC GAGTACTTTG TGGAAGGAGA TACATGGAAC 
ATTGATTCTT GCACACAATG TACTTGTCAT AATGCACGAG TTTTGTGCGA GACTGAAGTC 
TGTCCCCCTC TTCTTTGTCG AAATCCCACC CGTTCGCAAG ATTCATGCTG TCCACAGTGC 
CCAGATGAGC CCGCTCAGCC TTCCTTGTCA AGCAACGAGA GCATGCCTAG CTACTGCAAA 
AATGATGAAG GTGATATTTT CCTGACTGCT GAATCCTGGA AACCAAATGT TTGTGCTAGC 
TGTGTTTGCA TGGATGGTAC TATCAGCTGC TACTCTGAGT CTTGCCCACC AGTGTCCTGT 
GACAGGCCTG TTCTGAGAAA GGGCCAGTGT TGCCCTTATT GTATAGAAGA CACAACATCA 
AAGAAAGCAG TTTGTCACTT TAATGGGAAA ACATATGCAG ATGAAGAACG TTGGGACATT 
GACAGCTGCA CACACTGCTA CTGCCTGCAG GGTCAGACCC TTTGTTCCAC TGTCAGCTGC 
CCTCCTCTTC CTTGTGTGGA ACCCATCAAT GTTGAAGGAA GTTGCTGCCC CATGTGCCCA 
GAGATGTATG TACCTGAACC TACCAACATT CCAATTGAGA AGACAAACCA TCGAGAATAT 
AATGAACTGG AAGGACCAGT TTGGCCTACG CTCAATGAAA ATGACATTGT TCGTATCCAT 
AGAGATATGG GTCACCTTCA GGGGGATTTT AGAGAAAACA GAGGATCAGA ATCAAGCACA 
GAGGAATCTC TTAGCTCTCT TGCTTGGGTG ACGGTGCCCA TCATGACAGC CCTTGTTCTT 
ATCATAGCAC TTCTACTTGT CAATCAGAAG AAACAGTGGA TACCAGTATC CTGCTACAGA 
GCCCCATCAA AGCCTTCCTG CCTAAACAAT CAACTGGTAT ATGTGGACTG CAAGAAAGGA 
ACTATGGTTC AGGTAGACAG TTCTCAGAGG ATGCTAAGAA TTGCTGACCC AGATTCAAGA 
TACAGTGGAT TCTACAGCAT GCAAAAACAG AACAACTTAC AGGCAGATAA TTTCTATCAA 
ACAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_015423500.1 ) cDNA ORF clone, Gekko japonicus -> XP_015278986.1 Gekko japonicus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGe15575
Clone ID Related Accession (Same CDS sequence)	XM_015423500.1
Accession Version	XM_015423500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015159725.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGG CAACGCTGTC GGCGGGGCGG AGGCGCCGGG GCAGCGGCGG CGACTGGCAC 
CTGGCGGCGG GGCGGCTCCT GTTCCTGGCG CTGCTGCTCT TGGGGGAGTC CGGGACGCGG 
GCGTTCGTCT GCCTGCCGTG CGACGAGTCC AGGTGCGAGG AGCCCAAGAA CTGCCCCGGC 
GGCGTCGTCC TGGACATCTG CGGCTGCTGC TTCATGTGTG CCCGGCAACG CAACGAGAGT 
TGTGGGGGCT TCTTTGGGCT CCACGGAGCC TGCGACAAGG GGCTGCGCTG CGTGATCCGA 
CCCCCGCTCA ACGGAGACTC CCTCACCGAA TACGAAGCGG GCGTCTGTGA AGATGAAAAC 
TGGGATGATC AGCTACTTGG ATTTGAGCCT TGCAATGAAA ACCTGATAAC AGGCTGCAAT 
ATAATCAATG GAAAATGCGA ATGTGATACT ATTAGGACCT GTAGTAATCC CTTTGAGTTT 
CCAAGCCGGG ATACTTGTCT ATCAGCTTTA AAAAAGATTG AAGTTTTCAG CGTAGACTGC 
AGTACAGTAG AGTGTCCTCC TGTCCAGCAA GTTGTTTGCC CCTTGGATAG CTATGAAACC 
CAAGTCAGGC TGACAGCTGA TGGATGTTGT ACTCTTCCTA CAAGGTGCGA GTGTCTTTCT 
GGTTTATGTG GTATTCCAAA ATGTGAGGCA GGCTACATCC CCCAAATAGT GTCACGTGGA 
GACGGAACGC CTGGCAAGTG CTGTGATGTT TTTGAATGCG TCAGTGAAGT GAAGCCAGCC 
TGTATATATA ATAGTGTGGA ATATTATGAT GGAGATATGT TTCGAATGGA TGCTTGCCGT 
TTTTGTCGGT GTCAAGGTGG CGTCTCTATT TGTTTCTCCG CACAGTGTGG GGAGCTACAT 
TGTGAGAGAT ACTATGTACC AGAAGGAGAG TGCTGTCCAG TATGCGAAGA TTCGGTTTCT 
CCAGTTAGTA ACCCGGCTGG CTGTTATGCT GATGGCCAGA TCCAAGCACA TGGTGATCGC 
TGGAGGGAAG ATGATTGTAC TTTCTGCCAG TGTATCAATG GAGAGTCCCA TTGTGTTGCC 
ACAGCCTGTG GACAGAACTG CTTGAATCCT GTAAAAGTTC CTGGGGAATG CTGCCCAATG 
TGTGAAGAAC CAACTTATAT CACAATAGGT CCTCCCACAT GTTATCCTCT CGTGAACTGC 
ACGTTAACTG AGAGAGACTG TGTCTACAAC TTCAGAGTTG ACCAGAATGG TTGTCGCATC 
TGTCAATGCA AAACAAGAGA GGAGCTGTGT ATTGACCTCA TGGAAGGTTG TTCCTTGGTT 
TGTCCCTTCG GTTTCCAGAC TGATGACCGC AACTGTAAGA TCTGTCAGTG CCGACCACGA 
CCTAAGAAAT GCAAGCCCAT AGTATGTGAC AAGTACTGTC CATCTGGATA CCTGAAGAAC 
AAACATGGAT GTGAAATCTG TCGATGTAAG AAATGCCCAG AACAGTCATG CGACAAGATC 
TGCCCTAATG GCTTCCAGCA GGATAATGAA GGCTGTCTTA TCTGCAAATG TAGAGAGTTA 
CCAGCTTCTG TAATGCCACC AGCTAAGACT GGTTCTTGCC TGTCAGTGGA TGGGCACCGT 
CATGAAAATG AAGAAAGTTG GCATGATGGC TGCCGTGAAT GTTACTGCCA TAATGGACGG 
GAAATGTGTG CTTTGATCAC CTGCTCTGTA CCCAATTGTG GAAATCCCAC CATACATCCA 
GGCAAATGTT GTCCATCATG TCCAGATGAA TCTACTTTAC AGAAGCCAGA ACTCAGTGGT 
CCGACTATCT GCTATGTTCC AGGTGGCGAG TACTTTGTGG AAGGAGATAC ATGGAACATT 
GATTCTTGCA CACAATGTAC TTGTCATAAT GCACGAGTTT TGTGCGAGAC TGAAGTCTGT 
CCCCCTCTTC TTTGTCGAAA TCCCACCCGT TCGCAAGATT CATGCTGTCC ACAGTGCCCA 
GATGAGCCCG CTCAGCCTTC CTTGTCAAGC AACGAGAGCA TGCCTAGCTA CTGCAAAAAT 
GATGAAGGTG ATATTTTCCT GACTGCTGAA TCCTGGAAAC CAAATGTTTG TGCTAGCTGT 
GTTTGCATGG ATGGTACTAT CAGCTGCTAC TCTGAGTCTT GCCCACCAGT GTCCTGTGAC 
AGGCCTGTTC TGAGAAAGGG CCAGTGTTGC CCTTATTGTA TAGAAGACAC AACATCAAAG 
AAAGCAGTTT GTCACTTTAA TGGGAAAACA TATGCAGATG AAGAACGTTG GGACATTGAC 
AGCTGCACAC ACTGCTACTG CCTGCAGGGT CAGACCCTTT GTTCCACTGT CAGCTGCCCT 
CCTCTTCCTT GTGTGGAACC CATCAATGTT GAAGGAAGTT GCTGCCCCAT GTGCCCAGAG 
ATGTATGTAC CTGAACCTAC CAACATTCCA ATTGAGAAGA CAAACCATCG AGAATATAAT 
GAACTGGAAG GACCAGTTTG GCCTACGCTC AATGAAAATG ACATTGTTCG TATCCATAGA 
GATATGGGTC ACCTTCAGGG GGATTTTAGA GAAAACAGAG GATCAGAATC AAGCACAGAG 
GAATCTCTTA GCTCTCTTGC TTGGGTGACG GTGCCCATCA TGACAGCCCT TGTTCTTATC 
ATAGCACTTC TACTTGTCAA TCAGAAGAAA CAGTGGATAC CAGTATCCTG CTACAGAGCC 
CCATCAAAGC CTTCCTGCCT AAACAATCAA CTGGTATATG TGGACTGCAA GAAAGGAACT 
ATGGTTCAGG TAGACAGTTC TCAGAGGATG CTAAGAATTG CTGACCCAGA TTCAAGATAC 
AGTGGATTCT ACAGCATGCA AAAACAGAAC AACTTACAGG CAGATAATTT CTATCAAACA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015278986.1
CDS	42..2927
Translation	MYLATLSAGRRRRGSGGDWHLAAGRLLFLALLLLGESGTRAFVCLPCDESRCEEPKNCPGGVVLDICGCCFMCARQRNESCGGFFGLHGACDKGLRCVIRPPLNGDSLTEYEAGVCEDENWDDQLLGFEPCNENLITGCNIINGKCECDTIRTCSNPFEFPSRDTCLSALKKIEVFSVDCSTVECPPVQQVVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGIPKCEAGYIPQIVSRGDGTPGKCCDVFECVSEVKPACIYNSVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCERYYVPEGECCPVCEDSVSPVSNPAGCYADGQIQAHGDRWREDDCTFCQCINGESHCVATACGQNCLNPVKVPGECCPMCEEPTYITIGPPTCYPLVNCTLTERDCVYNFRVDQNGCRICQCKTREELCIDLMEGCSLVCPFGFQTDDRNCKICQCRPRPKKCKPIVCDKYCPSGYLKNKHGCEICRCKKCPEQSCDKICPNGFQQDNEGCLICKCRELPASVMPPAKTGSCLSVDGHRHENEESWHDGCRECYCHNGREMCALITCSVPNCGNPTIHPGKCCPSCPDESTLQKPELSGPTICYVPGGEYFVEGDTWNIDSCTQCTCHNARVLCETEVCPPLLCRNPTRSQDSCCPQCPDEPAQPSLSSNESMPSYCKNDEGDIFLTAESWKPNVCASCVCMDGTISCYSESCPPVSCDRPVLRKGQCCPYCIEDTTSKKAVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHREYNELEGPVWPTLNENDIVRIHRDMGHLQGDFRENRGSESSTEESLSSLAWVTVPIMTALVLIIALLLVNQKKQWIPVSCYRAPSKPSCLNNQLVYVDCKKGTMVQVDSSQRMLRIADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_015423500.1
Organism	Gekko japonicus
Definition	Gekko japonicus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015423500.1

ORF Insert Sequence:

ATGTACCTGG CAACGCTGTC GGCGGGGCGG AGGCGCCGGG GCAGCGGCGG CGACTGGCAC 
CTGGCGGCGG GGCGGCTCCT GTTCCTGGCG CTGCTGCTCT TGGGGGAGTC CGGGACGCGG 
GCGTTCGTCT GCCTGCCGTG CGACGAGTCC AGGTGCGAGG AGCCCAAGAA CTGCCCCGGC 
GGCGTCGTCC TGGACATCTG CGGCTGCTGC TTCATGTGTG CCCGGCAACG CAACGAGAGT 
TGTGGGGGCT TCTTTGGGCT CCACGGAGCC TGCGACAAGG GGCTGCGCTG CGTGATCCGA 
CCCCCGCTCA ACGGAGACTC CCTCACCGAA TACGAAGCGG GCGTCTGTGA AGATGAAAAC 
TGGGATGATC AGCTACTTGG ATTTGAGCCT TGCAATGAAA ACCTGATAAC AGGCTGCAAT 
ATAATCAATG GAAAATGCGA ATGTGATACT ATTAGGACCT GTAGTAATCC CTTTGAGTTT 
CCAAGCCGGG ATACTTGTCT ATCAGCTTTA AAAAAGATTG AAGTTTTCAG CGTAGACTGC 
AGTACAGTAG AGTGTCCTCC TGTCCAGCAA GTTGTTTGCC CCTTGGATAG CTATGAAACC 
CAAGTCAGGC TGACAGCTGA TGGATGTTGT ACTCTTCCTA CAAGGTGCGA GTGTCTTTCT 
GGTTTATGTG GTATTCCAAA ATGTGAGGCA GGCTACATCC CCCAAATAGT GTCACGTGGA 
GACGGAACGC CTGGCAAGTG CTGTGATGTT TTTGAATGCG TCAGTGAAGT GAAGCCAGCC 
TGTATATATA ATAGTGTGGA ATATTATGAT GGAGATATGT TTCGAATGGA TGCTTGCCGT 
TTTTGTCGGT GTCAAGGTGG CGTCTCTATT TGTTTCTCCG CACAGTGTGG GGAGCTACAT 
TGTGAGAGAT ACTATGTACC AGAAGGAGAG TGCTGTCCAG TATGCGAAGA TTCGGTTTCT 
CCAGTTAGTA ACCCGGCTGG CTGTTATGCT GATGGCCAGA TCCAAGCACA TGGTGATCGC 
TGGAGGGAAG ATGATTGTAC TTTCTGCCAG TGTATCAATG GAGAGTCCCA TTGTGTTGCC 
ACAGCCTGTG GACAGAACTG CTTGAATCCT GTAAAAGTTC CTGGGGAATG CTGCCCAATG 
TGTGAAGAAC CAACTTATAT CACAATAGGT CCTCCCACAT GTTATCCTCT CGTGAACTGC 
ACGTTAACTG AGAGAGACTG TGTCTACAAC TTCAGAGTTG ACCAGAATGG TTGTCGCATC 
TGTCAATGCA AAACAAGAGA GGAGCTGTGT ATTGACCTCA TGGAAGGTTG TTCCTTGGTT 
TGTCCCTTCG GTTTCCAGAC TGATGACCGC AACTGTAAGA TCTGTCAGTG CCGACCACGA 
CCTAAGAAAT GCAAGCCCAT AGTATGTGAC AAGTACTGTC CATCTGGATA CCTGAAGAAC 
AAACATGGAT GTGAAATCTG TCGATGTAAG AAATGCCCAG AACAGTCATG CGACAAGATC 
TGCCCTAATG GCTTCCAGCA GGATAATGAA GGCTGTCTTA TCTGCAAATG TAGAGAGTTA 
CCAGCTTCTG TAATGCCACC AGCTAAGACT GGTTCTTGCC TGTCAGTGGA TGGGCACCGT 
CATGAAAATG AAGAAAGTTG GCATGATGGC TGCCGTGAAT GTTACTGCCA TAATGGACGG 
GAAATGTGTG CTTTGATCAC CTGCTCTGTA CCCAATTGTG GAAATCCCAC CATACATCCA 
GGCAAATGTT GTCCATCATG TCCAGATGAA TCTACTTTAC AGAAGCCAGA ACTCAGTGGT 
CCGACTATCT GCTATGTTCC AGGTGGCGAG TACTTTGTGG AAGGAGATAC ATGGAACATT 
GATTCTTGCA CACAATGTAC TTGTCATAAT GCACGAGTTT TGTGCGAGAC TGAAGTCTGT 
CCCCCTCTTC TTTGTCGAAA TCCCACCCGT TCGCAAGATT CATGCTGTCC ACAGTGCCCA 
GATGAGCCCG CTCAGCCTTC CTTGTCAAGC AACGAGAGCA TGCCTAGCTA CTGCAAAAAT 
GATGAAGGTG ATATTTTCCT GACTGCTGAA TCCTGGAAAC CAAATGTTTG TGCTAGCTGT 
GTTTGCATGG ATGGTACTAT CAGCTGCTAC TCTGAGTCTT GCCCACCAGT GTCCTGTGAC 
AGGCCTGTTC TGAGAAAGGG CCAGTGTTGC CCTTATTGTA TAGAAGACAC AACATCAAAG 
AAAGCAGTTT GTCACTTTAA TGGGAAAACA TATGCAGATG AAGAACGTTG GGACATTGAC 
AGCTGCACAC ACTGCTACTG CCTGCAGGGT CAGACCCTTT GTTCCACTGT CAGCTGCCCT 
CCTCTTCCTT GTGTGGAACC CATCAATGTT GAAGGAAGTT GCTGCCCCAT GTGCCCAGAG 
ATGTATGTAC CTGAACCTAC CAACATTCCA ATTGAGAAGA CAAACCATCG AGAATATAAT 
GAACTGGAAG GACCAGTTTG GCCTACGCTC AATGAAAATG ACATTGTTCG TATCCATAGA 
GATATGGGTC ACCTTCAGGG GGATTTTAGA GAAAACAGAG GATCAGAATC AAGCACAGAG 
GAATCTCTTA GCTCTCTTGC TTGGGTGACG GTGCCCATCA TGACAGCCCT TGTTCTTATC 
ATAGCACTTC TACTTGTCAA TCAGAAGAAA CAGTGGATAC CAGTATCCTG CTACAGAGCC 
CCATCAAAGC CTTCCTGCCT AAACAATCAA CTGGTATATG TGGACTGCAA GAAAGGAACT 
ATGGTTCAGG TAGACAGTTC TCAGAGGATG CTAAGAATTG CTGACCCAGA TTCAAGATAC 
AGTGGATTCT ACAGCATGCA AAAACAGAAC AACTTACAGG CAGATAATTT CTATCAAACA 
GTGTGA