AGTPBP1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	AGTPBP1
Entrez Gene ID	107117203
Full Name	ATP/GTP binding protein 1
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015419280.1	XP_015274766.1	cytosolic carboxypeptidase 1
XM_015419279.1	XP_015274765.1	cytosolic carboxypeptidase 1

Pathways from BioSystems
Gene Ontology

The following AGTPBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGTPBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe28733	XM_015419279.1 Latest version!	Gekko japonicus ATP/GTP binding protein 1 (AGTPBP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGe28733	XM_015419280.1 Latest version!	Gekko japonicus ATP/GTP binding protein 1 (AGTPBP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGTPBP1 ( XM_015419280.1 ) cDNA ORF clone, Gekko japonicus -> XP_015274766.1 Gekko japonicus ATP/GTP binding protein 1 (AGTPBP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGe28733
Clone ID Related Accession (Same CDS sequence)	XM_015419280.1 , XM_015419279.1
Accession Version	XM_015419280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	cytosolic carboxypeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015171506.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAT TAAGATCGAC ATCAGAAAAA AGTATAACTA ACAATTCCCG GATTGCAAGT 
TTATTGACAC AACTTGAGAA GATCAATGCA GAATGTTTGG TGGAAACCGA CACCGCCAGA 
TATGTTACAT CAAAGATACT CCATCTAACT CAAAGTCAAG AAAAAATAAA GAAGGAGATG 
ACTGCAAAAG GTTCAACAGG CATTGAAGTT ATTCTCTCAA CTTTAGAGAA TACAAGAGAT 
CTTCAGACTA CTCTAAATAT CTTAAACATC CTGATTGAGT TGGTAGCAGT CGGTGGTAGC 
CGGAGAACAA GTATTCTCGT TTCCAAAGGA GGAACACAGA TCTTACTGCA ATTGCTTGTG 
AATGTCAGTA AAGATTCCCC ACCAAATGAA GACTTAATGG TGCTACTTCA TTCTCTCCTT 
GCAAAAATTG GTCCAAAAGA TAAAAAAATT GGAGTAAAAG CCAGAATTAG CGGTGCTTTA 
AATATAACAC TAAATATAGT GAAGCAGAAC TTGCAGAATC AAAGATTAAT ACTGCCTTGT 
CTTCAAGTGT TACGAGTATA TTCAACCAAC TCTGTAAATG CTGTGTCCTT GGGTAAGAAT 
GGAATTGTGG AGCTGATGTT TAAGATCATC GGTCCTTTCA GCAAAAAGAA CACTGGCCTT 
ATGAAGGTTG CTTTAGACAC TTTTGCTGCA TTGCTAAGAT CGAAAACAAA TGCTAGGAGA 
GCAGTAGACA GAGGCTATGT CCACGTGCTT TTAACAATCT ATGTAGACTG GCACCGTCAT 
GATAGCCGCC ATAGACATAT GTTGATCCGC AAAGGAATCT TGCAGTGTAT TAAAAATGTT 
ACGAACACCA AGCTGGGAAG AAAAGCATTT CTTGATGTCA GTGGGATGAA AATTCTGTAC 
AACACATCAC AAGAATGCCT TGCAGTAAGA ACTCTTGATC CATTAGTCAA CACTTCCAGT 
CTGATAATGA GAAAATGTTA TCCTAAAAAT CGTCTTCCGC TGCCAACCAT TAAAAGTGCT 
TTCCATTTCC AATTACCTGT CATCCCTGGT AATGGACCTG TGGCTCAGCT GTATAGTTTG 
CCTCCTGAAG TGGATGACGT AGTAGATGAA AGTGATGACA ATGATGACAC CGAAACAGAT 
ACTGAGATTG AAAATGGTAA TGAAGATGAC ATAGAGCAGC ATTTTAAGAA TGATGATATA 
GAGACTGATA TCAACAAGTT GAAACCAAAG CAGGAGTTGG GACGACCTAT AGAAGATCTA 
AAGATGTATG AAAATTTTTT CCCAGAACTT GTGGAGGATT TTCAGGAATA CGATTTAGTT 
TCCAAAGAAC CAAAGCCTTT TTCAACTGAT GTATCACTAG GAGGACCTAT TATTGTTCCT 
ACCGCTGGTG ATGAACAGTC TCCCACAAAT CATATCATAG AAATTCCTGT GAAGGGGAAT 
CAGCCTTTGC AAATGGAGGA CTACAATAAA AGACCCATCT CTTTGGGTCT GGTAAAGAAA 
AATAGCAACA CAGACAATAG TTTACAGCAT CAAGGTGATC ATTTGAAATT ACATTCATCG 
TGCCAGTGCC TAAACCATGA AATAGTCAAA GGCTTGGACA GAATTACTTT GCAGAACACT 
CCCAAAAATG AACAGTCTTA CAGTTCAAGA GCAACAACCC AAAAAGAGTG TAAAACTAGC 
TGTAGTGAAG TTACCTGTAG TAAACCCTTA CAACATGTTT CATCCTGTGG AAGTGTTTTG 
TTTGAGGGAC GGTCTGTTCA GCTGGGAAAG CTGTGCTGTT CTGGTGCTGA TCCTGAAGAG 
GAGGAAGTAC CTTGCTCAGT TTCTTTGGGA GAGCAAGTGA TGCTTGATGT GCCTGATCTA 
TTACAACTCC ATGACCCAGA ACTTTATATT GAGATAGTGA AAAATACAAA GTCTGTTCCT 
GAATATTCAG AAGTGGCTTA TCCTGACTAT TTTGGACACT TGCCACCGCC ATTTAAAGAG 
CCCATTCTTG AAAGGCCGTA TGGTGTTCAA CGGACAAAAA TTGCCCAGGA TATTGAAAGG 
CTAATTCATC CTAATGATAT TATAGACAGA GTTGTGTATG ACCTTGACAG TCCCAGCTAC 
ATGGTGCCAG ATGAAGGAGA CATTTTGAAG TTTAATTCTA AATTTGAATC AGGGAACTTG 
CGCAAAGTCA TTCAGATCAG AAAAAATGAG TATGATCTTA TCCTGAATGC TGATATAAAC 
AGCAACCATT ATCACCAGTG GTTTTACTTC GAAGTCAGTG GAATGAGGAC AGGCATTGCT 
TACCGATTTA ATATAATCAA CTGTGAAAAG TCAAACAGTC AGTTCAATTA TGGTATGCAG 
CCCCTCATGT ACTCTGTTCA AGATGCATTA AATGGCCAAC CCTGGTGGAT TCGAGTAGGA 
ACAGATATTT GTTACTACAA GAATCATTTT TCTAGAAGCT CAGCTGCTGC TGGAGGTCAG 
AAGGGAAAAT CCTACTATAC AACTACCTTC ACTGTTAGCT TCCCACACAA GGAAGATGTT 
TGCTACTTCG CTTACCATTA TCCATATACT TATTCAACTT TAATGATGCA CCTTCAAAAA 
TTGGAATCTA CATACAATCC TCAGCAGATA TATTTCCGAC ACGATGTTTT GTGTGAGACC 
CTTTCTGGAA ACAACTGTCC TTTACTCACT ATAACAGCCA TGCCAGAATC AAATTACTAT 
GAACATATCT GTCAGTTCAG AAACCGTCCT TTTATATTCT TATCTGCTCG TGTACATCCT 
GGAGAAACTA ATGCAAGCTG GGTTATGAAG GGAACACTGG AATATCTCAT GGGTAATAAT 
CCCACTGCCC AGGCATTACG AGAATCTTAT ATTTTTAAAA TTGTTCCAAT GCTTAACCCA 
GATGGGGTCA TCAATGGAAA CCATCGCTGT TCTTTAAGTG GGGAGGACTT GAATAGACAA 
TGGCAAAACC CAAACCCAGA TCTGCATCCT GCAATTTATC ATGCCAAGGG TCTCCTCCAG 
TACCTGTCTG CTATAAAGCG TTTGCCACTG GTTTACTGTG ATTATCATGG TCATTCTCGC 
AAGAAGAATG TGTTCATGTA TGGCTGCAGT ATCAAGGAAA CAATGTGGCA CACAAATGTT 
AGTGCAGCTT CTTGTGATCT GATTGAAGAC TTAAGTTACA GGACACTCCC TAAGATTCTG 
AGCCAGACAG CTCCAGCATT CTGCATGAGC AGCTGCAGTT TTGTGGTGGA AAAGTCTAAA 
GAATCTACAG CACGTGTTGT AGTCTGGAGA GAGATAGGTG TTCAGAGAAG TTATACCATG 
GAAAGCACAT TGTGTGGATG TGATCAAGGG AAATACAAGG GTTTGCAGAT TGGGACACGT 
GAGCTTGAAG AGATGGGAGC TAAATTCTGT TCTGCTTTAC TGCGTTTGAA AAGATTGACA 
TCGCCACTGG AATACAACCT ACCACCTAGC CTCTTTGACA TTGAAAATGA TTTGATTGAG 
TCCAGTTGTA AAGTAACAAG CCCCACTACA TATGTAATGG AAGAAGATGA GCCTCGCTTC 
CTTGAAGAAG TGGATTATAG TGCTGAAAGC AATGATGAGC CAGATGTTGA ATTGACAAAT 
AATGCTGGTG ACTATGAGCC CAGTCTTCAA GAAGATGGAC TTTCTGACTC TGAAATTACA 
AGGACACATT TGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015274766.1
CDS	98..3775
Translation	MNKLRSTSEKSITNNSRIASLLTQLEKINAECLVETDTARYVTSKILHLTQSQEKIKKEMTAKGSTGIEVILSTLENTRDLQTTLNILNILIELVAVGGSRRTSILVSKGGTQILLQLLVNVSKDSPPNEDLMVLLHSLLAKIGPKDKKIGVKARISGALNITLNIVKQNLQNQRLILPCLQVLRVYSTNSVNAVSLGKNGIVELMFKIIGPFSKKNTGLMKVALDTFAALLRSKTNARRAVDRGYVHVLLTIYVDWHRHDSRHRHMLIRKGILQCIKNVTNTKLGRKAFLDVSGMKILYNTSQECLAVRTLDPLVNTSSLIMRKCYPKNRLPLPTIKSAFHFQLPVIPGNGPVAQLYSLPPEVDDVVDESDDNDDTETDTEIENGNEDDIEQHFKNDDIETDINKLKPKQELGRPIEDLKMYENFFPELVEDFQEYDLVSKEPKPFSTDVSLGGPIIVPTAGDEQSPTNHIIEIPVKGNQPLQMEDYNKRPISLGLVKKNSNTDNSLQHQGDHLKLHSSCQCLNHEIVKGLDRITLQNTPKNEQSYSSRATTQKECKTSCSEVTCSKPLQHVSSCGSVLFEGRSVQLGKLCCSGADPEEEEVPCSVSLGEQVMLDVPDLLQLHDPELYIEIVKNTKSVPEYSEVAYPDYFGHLPPPFKEPILERPYGVQRTKIAQDIERLIHPNDIIDRVVYDLDSPSYMVPDEGDILKFNSKFESGNLRKVIQIRKNEYDLILNADINSNHYHQWFYFEVSGMRTGIAYRFNIINCEKSNSQFNYGMQPLMYSVQDALNGQPWWIRVGTDICYYKNHFSRSSAAAGGQKGKSYYTTTFTVSFPHKEDVCYFAYHYPYTYSTLMMHLQKLESTYNPQQIYFRHDVLCETLSGNNCPLLTITAMPESNYYEHICQFRNRPFIFLSARVHPGETNASWVMKGTLEYLMGNNPTAQALRESYIFKIVPMLNPDGVINGNHRCSLSGEDLNRQWQNPNPDLHPAIYHAKGLLQYLSAIKRLPLVYCDYHGHSRKKNVFMYGCSIKETMWHTNVSAASCDLIEDLSYRTLPKILSQTAPAFCMSSCSFVVEKSKESTARVVVWREIGVQRSYTMESTLCGCDQGKYKGLQIGTRELEEMGAKFCSALLRLKRLTSPLEYNLPPSLFDIENDLIESSCKVTSPTTYVMEEDEPRFLEEVDYSAESNDEPDVELTNNAGDYEPSLQEDGLSDSEITRTHLP

Target ORF information:

RefSeq Version	XM_015419280.1
Organism	Gekko japonicus
Definition	Gekko japonicus ATP/GTP binding protein 1 (AGTPBP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015419280.1

ORF Insert Sequence:

ATGAATAAAT TAAGATCGAC ATCAGAAAAA AGTATAACTA ACAATTCCCG GATTGCAAGT 
TTATTGACAC AACTTGAGAA GATCAATGCA GAATGTTTGG TGGAAACCGA CACCGCCAGA 
TATGTTACAT CAAAGATACT CCATCTAACT CAAAGTCAAG AAAAAATAAA GAAGGAGATG 
ACTGCAAAAG GTTCAACAGG CATTGAAGTT ATTCTCTCAA CTTTAGAGAA TACAAGAGAT 
CTTCAGACTA CTCTAAATAT CTTAAACATC CTGATTGAGT TGGTAGCAGT CGGTGGTAGC 
CGGAGAACAA GTATTCTCGT TTCCAAAGGA GGAACACAGA TCTTACTGCA ATTGCTTGTG 
AATGTCAGTA AAGATTCCCC ACCAAATGAA GACTTAATGG TGCTACTTCA TTCTCTCCTT 
GCAAAAATTG GTCCAAAAGA TAAAAAAATT GGAGTAAAAG CCAGAATTAG CGGTGCTTTA 
AATATAACAC TAAATATAGT GAAGCAGAAC TTGCAGAATC AAAGATTAAT ACTGCCTTGT 
CTTCAAGTGT TACGAGTATA TTCAACCAAC TCTGTAAATG CTGTGTCCTT GGGTAAGAAT 
GGAATTGTGG AGCTGATGTT TAAGATCATC GGTCCTTTCA GCAAAAAGAA CACTGGCCTT 
ATGAAGGTTG CTTTAGACAC TTTTGCTGCA TTGCTAAGAT CGAAAACAAA TGCTAGGAGA 
GCAGTAGACA GAGGCTATGT CCACGTGCTT TTAACAATCT ATGTAGACTG GCACCGTCAT 
GATAGCCGCC ATAGACATAT GTTGATCCGC AAAGGAATCT TGCAGTGTAT TAAAAATGTT 
ACGAACACCA AGCTGGGAAG AAAAGCATTT CTTGATGTCA GTGGGATGAA AATTCTGTAC 
AACACATCAC AAGAATGCCT TGCAGTAAGA ACTCTTGATC CATTAGTCAA CACTTCCAGT 
CTGATAATGA GAAAATGTTA TCCTAAAAAT CGTCTTCCGC TGCCAACCAT TAAAAGTGCT 
TTCCATTTCC AATTACCTGT CATCCCTGGT AATGGACCTG TGGCTCAGCT GTATAGTTTG 
CCTCCTGAAG TGGATGACGT AGTAGATGAA AGTGATGACA ATGATGACAC CGAAACAGAT 
ACTGAGATTG AAAATGGTAA TGAAGATGAC ATAGAGCAGC ATTTTAAGAA TGATGATATA 
GAGACTGATA TCAACAAGTT GAAACCAAAG CAGGAGTTGG GACGACCTAT AGAAGATCTA 
AAGATGTATG AAAATTTTTT CCCAGAACTT GTGGAGGATT TTCAGGAATA CGATTTAGTT 
TCCAAAGAAC CAAAGCCTTT TTCAACTGAT GTATCACTAG GAGGACCTAT TATTGTTCCT 
ACCGCTGGTG ATGAACAGTC TCCCACAAAT CATATCATAG AAATTCCTGT GAAGGGGAAT 
CAGCCTTTGC AAATGGAGGA CTACAATAAA AGACCCATCT CTTTGGGTCT GGTAAAGAAA 
AATAGCAACA CAGACAATAG TTTACAGCAT CAAGGTGATC ATTTGAAATT ACATTCATCG 
TGCCAGTGCC TAAACCATGA AATAGTCAAA GGCTTGGACA GAATTACTTT GCAGAACACT 
CCCAAAAATG AACAGTCTTA CAGTTCAAGA GCAACAACCC AAAAAGAGTG TAAAACTAGC 
TGTAGTGAAG TTACCTGTAG TAAACCCTTA CAACATGTTT CATCCTGTGG AAGTGTTTTG 
TTTGAGGGAC GGTCTGTTCA GCTGGGAAAG CTGTGCTGTT CTGGTGCTGA TCCTGAAGAG 
GAGGAAGTAC CTTGCTCAGT TTCTTTGGGA GAGCAAGTGA TGCTTGATGT GCCTGATCTA 
TTACAACTCC ATGACCCAGA ACTTTATATT GAGATAGTGA AAAATACAAA GTCTGTTCCT 
GAATATTCAG AAGTGGCTTA TCCTGACTAT TTTGGACACT TGCCACCGCC ATTTAAAGAG 
CCCATTCTTG AAAGGCCGTA TGGTGTTCAA CGGACAAAAA TTGCCCAGGA TATTGAAAGG 
CTAATTCATC CTAATGATAT TATAGACAGA GTTGTGTATG ACCTTGACAG TCCCAGCTAC 
ATGGTGCCAG ATGAAGGAGA CATTTTGAAG TTTAATTCTA AATTTGAATC AGGGAACTTG 
CGCAAAGTCA TTCAGATCAG AAAAAATGAG TATGATCTTA TCCTGAATGC TGATATAAAC 
AGCAACCATT ATCACCAGTG GTTTTACTTC GAAGTCAGTG GAATGAGGAC AGGCATTGCT 
TACCGATTTA ATATAATCAA CTGTGAAAAG TCAAACAGTC AGTTCAATTA TGGTATGCAG 
CCCCTCATGT ACTCTGTTCA AGATGCATTA AATGGCCAAC CCTGGTGGAT TCGAGTAGGA 
ACAGATATTT GTTACTACAA GAATCATTTT TCTAGAAGCT CAGCTGCTGC TGGAGGTCAG 
AAGGGAAAAT CCTACTATAC AACTACCTTC ACTGTTAGCT TCCCACACAA GGAAGATGTT 
TGCTACTTCG CTTACCATTA TCCATATACT TATTCAACTT TAATGATGCA CCTTCAAAAA 
TTGGAATCTA CATACAATCC TCAGCAGATA TATTTCCGAC ACGATGTTTT GTGTGAGACC 
CTTTCTGGAA ACAACTGTCC TTTACTCACT ATAACAGCCA TGCCAGAATC AAATTACTAT 
GAACATATCT GTCAGTTCAG AAACCGTCCT TTTATATTCT TATCTGCTCG TGTACATCCT 
GGAGAAACTA ATGCAAGCTG GGTTATGAAG GGAACACTGG AATATCTCAT GGGTAATAAT 
CCCACTGCCC AGGCATTACG AGAATCTTAT ATTTTTAAAA TTGTTCCAAT GCTTAACCCA 
GATGGGGTCA TCAATGGAAA CCATCGCTGT TCTTTAAGTG GGGAGGACTT GAATAGACAA 
TGGCAAAACC CAAACCCAGA TCTGCATCCT GCAATTTATC ATGCCAAGGG TCTCCTCCAG 
TACCTGTCTG CTATAAAGCG TTTGCCACTG GTTTACTGTG ATTATCATGG TCATTCTCGC 
AAGAAGAATG TGTTCATGTA TGGCTGCAGT ATCAAGGAAA CAATGTGGCA CACAAATGTT 
AGTGCAGCTT CTTGTGATCT GATTGAAGAC TTAAGTTACA GGACACTCCC TAAGATTCTG 
AGCCAGACAG CTCCAGCATT CTGCATGAGC AGCTGCAGTT TTGTGGTGGA AAAGTCTAAA 
GAATCTACAG CACGTGTTGT AGTCTGGAGA GAGATAGGTG TTCAGAGAAG TTATACCATG 
GAAAGCACAT TGTGTGGATG TGATCAAGGG AAATACAAGG GTTTGCAGAT TGGGACACGT 
GAGCTTGAAG AGATGGGAGC TAAATTCTGT TCTGCTTTAC TGCGTTTGAA AAGATTGACA 
TCGCCACTGG AATACAACCT ACCACCTAGC CTCTTTGACA TTGAAAATGA TTTGATTGAG 
TCCAGTTGTA AAGTAACAAG CCCCACTACA TATGTAATGG AAGAAGATGA GCCTCGCTTC 
CTTGAAGAAG TGGATTATAG TGCTGAAAGC AATGATGAGC CAGATGTTGA ATTGACAAAT 
AATGCTGGTG ACTATGAGCC CAGTCTTCAA GAAGATGGAC TTTCTGACTC TGAAATTACA 
AGGACACATT TGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGTPBP1 ( XM_015419279.1 ) cDNA ORF clone, Gekko japonicus -> XP_015274765.1 Gekko japonicus ATP/GTP binding protein 1 (AGTPBP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGe28733
Clone ID Related Accession (Same CDS sequence)	XM_015419280.1 , XM_015419279.1
Accession Version	XM_015419279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	cytosolic carboxypeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015171506.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAT TAAGATCGAC ATCAGAAAAA AGTATAACTA ACAATTCCCG GATTGCAAGT 
TTATTGACAC AACTTGAGAA GATCAATGCA GAATGTTTGG TGGAAACCGA CACCGCCAGA 
TATGTTACAT CAAAGATACT CCATCTAACT CAAAGTCAAG AAAAAATAAA GAAGGAGATG 
ACTGCAAAAG GTTCAACAGG CATTGAAGTT ATTCTCTCAA CTTTAGAGAA TACAAGAGAT 
CTTCAGACTA CTCTAAATAT CTTAAACATC CTGATTGAGT TGGTAGCAGT CGGTGGTAGC 
CGGAGAACAA GTATTCTCGT TTCCAAAGGA GGAACACAGA TCTTACTGCA ATTGCTTGTG 
AATGTCAGTA AAGATTCCCC ACCAAATGAA GACTTAATGG TGCTACTTCA TTCTCTCCTT 
GCAAAAATTG GTCCAAAAGA TAAAAAAATT GGAGTAAAAG CCAGAATTAG CGGTGCTTTA 
AATATAACAC TAAATATAGT GAAGCAGAAC TTGCAGAATC AAAGATTAAT ACTGCCTTGT 
CTTCAAGTGT TACGAGTATA TTCAACCAAC TCTGTAAATG CTGTGTCCTT GGGTAAGAAT 
GGAATTGTGG AGCTGATGTT TAAGATCATC GGTCCTTTCA GCAAAAAGAA CACTGGCCTT 
ATGAAGGTTG CTTTAGACAC TTTTGCTGCA TTGCTAAGAT CGAAAACAAA TGCTAGGAGA 
GCAGTAGACA GAGGCTATGT CCACGTGCTT TTAACAATCT ATGTAGACTG GCACCGTCAT 
GATAGCCGCC ATAGACATAT GTTGATCCGC AAAGGAATCT TGCAGTGTAT TAAAAATGTT 
ACGAACACCA AGCTGGGAAG AAAAGCATTT CTTGATGTCA GTGGGATGAA AATTCTGTAC 
AACACATCAC AAGAATGCCT TGCAGTAAGA ACTCTTGATC CATTAGTCAA CACTTCCAGT 
CTGATAATGA GAAAATGTTA TCCTAAAAAT CGTCTTCCGC TGCCAACCAT TAAAAGTGCT 
TTCCATTTCC AATTACCTGT CATCCCTGGT AATGGACCTG TGGCTCAGCT GTATAGTTTG 
CCTCCTGAAG TGGATGACGT AGTAGATGAA AGTGATGACA ATGATGACAC CGAAACAGAT 
ACTGAGATTG AAAATGGTAA TGAAGATGAC ATAGAGCAGC ATTTTAAGAA TGATGATATA 
GAGACTGATA TCAACAAGTT GAAACCAAAG CAGGAGTTGG GACGACCTAT AGAAGATCTA 
AAGATGTATG AAAATTTTTT CCCAGAACTT GTGGAGGATT TTCAGGAATA CGATTTAGTT 
TCCAAAGAAC CAAAGCCTTT TTCAACTGAT GTATCACTAG GAGGACCTAT TATTGTTCCT 
ACCGCTGGTG ATGAACAGTC TCCCACAAAT CATATCATAG AAATTCCTGT GAAGGGGAAT 
CAGCCTTTGC AAATGGAGGA CTACAATAAA AGACCCATCT CTTTGGGTCT GGTAAAGAAA 
AATAGCAACA CAGACAATAG TTTACAGCAT CAAGGTGATC ATTTGAAATT ACATTCATCG 
TGCCAGTGCC TAAACCATGA AATAGTCAAA GGCTTGGACA GAATTACTTT GCAGAACACT 
CCCAAAAATG AACAGTCTTA CAGTTCAAGA GCAACAACCC AAAAAGAGTG TAAAACTAGC 
TGTAGTGAAG TTACCTGTAG TAAACCCTTA CAACATGTTT CATCCTGTGG AAGTGTTTTG 
TTTGAGGGAC GGTCTGTTCA GCTGGGAAAG CTGTGCTGTT CTGGTGCTGA TCCTGAAGAG 
GAGGAAGTAC CTTGCTCAGT TTCTTTGGGA GAGCAAGTGA TGCTTGATGT GCCTGATCTA 
TTACAACTCC ATGACCCAGA ACTTTATATT GAGATAGTGA AAAATACAAA GTCTGTTCCT 
GAATATTCAG AAGTGGCTTA TCCTGACTAT TTTGGACACT TGCCACCGCC ATTTAAAGAG 
CCCATTCTTG AAAGGCCGTA TGGTGTTCAA CGGACAAAAA TTGCCCAGGA TATTGAAAGG 
CTAATTCATC CTAATGATAT TATAGACAGA GTTGTGTATG ACCTTGACAG TCCCAGCTAC 
ATGGTGCCAG ATGAAGGAGA CATTTTGAAG TTTAATTCTA AATTTGAATC AGGGAACTTG 
CGCAAAGTCA TTCAGATCAG AAAAAATGAG TATGATCTTA TCCTGAATGC TGATATAAAC 
AGCAACCATT ATCACCAGTG GTTTTACTTC GAAGTCAGTG GAATGAGGAC AGGCATTGCT 
TACCGATTTA ATATAATCAA CTGTGAAAAG TCAAACAGTC AGTTCAATTA TGGTATGCAG 
CCCCTCATGT ACTCTGTTCA AGATGCATTA AATGGCCAAC CCTGGTGGAT TCGAGTAGGA 
ACAGATATTT GTTACTACAA GAATCATTTT TCTAGAAGCT CAGCTGCTGC TGGAGGTCAG 
AAGGGAAAAT CCTACTATAC AACTACCTTC ACTGTTAGCT TCCCACACAA GGAAGATGTT 
TGCTACTTCG CTTACCATTA TCCATATACT TATTCAACTT TAATGATGCA CCTTCAAAAA 
TTGGAATCTA CATACAATCC TCAGCAGATA TATTTCCGAC ACGATGTTTT GTGTGAGACC 
CTTTCTGGAA ACAACTGTCC TTTACTCACT ATAACAGCCA TGCCAGAATC AAATTACTAT 
GAACATATCT GTCAGTTCAG AAACCGTCCT TTTATATTCT TATCTGCTCG TGTACATCCT 
GGAGAAACTA ATGCAAGCTG GGTTATGAAG GGAACACTGG AATATCTCAT GGGTAATAAT 
CCCACTGCCC AGGCATTACG AGAATCTTAT ATTTTTAAAA TTGTTCCAAT GCTTAACCCA 
GATGGGGTCA TCAATGGAAA CCATCGCTGT TCTTTAAGTG GGGAGGACTT GAATAGACAA 
TGGCAAAACC CAAACCCAGA TCTGCATCCT GCAATTTATC ATGCCAAGGG TCTCCTCCAG 
TACCTGTCTG CTATAAAGCG TTTGCCACTG GTTTACTGTG ATTATCATGG TCATTCTCGC 
AAGAAGAATG TGTTCATGTA TGGCTGCAGT ATCAAGGAAA CAATGTGGCA CACAAATGTT 
AGTGCAGCTT CTTGTGATCT GATTGAAGAC TTAAGTTACA GGACACTCCC TAAGATTCTG 
AGCCAGACAG CTCCAGCATT CTGCATGAGC AGCTGCAGTT TTGTGGTGGA AAAGTCTAAA 
GAATCTACAG CACGTGTTGT AGTCTGGAGA GAGATAGGTG TTCAGAGAAG TTATACCATG 
GAAAGCACAT TGTGTGGATG TGATCAAGGG AAATACAAGG GTTTGCAGAT TGGGACACGT 
GAGCTTGAAG AGATGGGAGC TAAATTCTGT TCTGCTTTAC TGCGTTTGAA AAGATTGACA 
TCGCCACTGG AATACAACCT ACCACCTAGC CTCTTTGACA TTGAAAATGA TTTGATTGAG 
TCCAGTTGTA AAGTAACAAG CCCCACTACA TATGTAATGG AAGAAGATGA GCCTCGCTTC 
CTTGAAGAAG TGGATTATAG TGCTGAAAGC AATGATGAGC CAGATGTTGA ATTGACAAAT 
AATGCTGGTG ACTATGAGCC CAGTCTTCAA GAAGATGGAC TTTCTGACTC TGAAATTACA 
AGGACACATT TGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015274765.1
CDS	314..3991
Translation	MNKLRSTSEKSITNNSRIASLLTQLEKINAECLVETDTARYVTSKILHLTQSQEKIKKEMTAKGSTGIEVILSTLENTRDLQTTLNILNILIELVAVGGSRRTSILVSKGGTQILLQLLVNVSKDSPPNEDLMVLLHSLLAKIGPKDKKIGVKARISGALNITLNIVKQNLQNQRLILPCLQVLRVYSTNSVNAVSLGKNGIVELMFKIIGPFSKKNTGLMKVALDTFAALLRSKTNARRAVDRGYVHVLLTIYVDWHRHDSRHRHMLIRKGILQCIKNVTNTKLGRKAFLDVSGMKILYNTSQECLAVRTLDPLVNTSSLIMRKCYPKNRLPLPTIKSAFHFQLPVIPGNGPVAQLYSLPPEVDDVVDESDDNDDTETDTEIENGNEDDIEQHFKNDDIETDINKLKPKQELGRPIEDLKMYENFFPELVEDFQEYDLVSKEPKPFSTDVSLGGPIIVPTAGDEQSPTNHIIEIPVKGNQPLQMEDYNKRPISLGLVKKNSNTDNSLQHQGDHLKLHSSCQCLNHEIVKGLDRITLQNTPKNEQSYSSRATTQKECKTSCSEVTCSKPLQHVSSCGSVLFEGRSVQLGKLCCSGADPEEEEVPCSVSLGEQVMLDVPDLLQLHDPELYIEIVKNTKSVPEYSEVAYPDYFGHLPPPFKEPILERPYGVQRTKIAQDIERLIHPNDIIDRVVYDLDSPSYMVPDEGDILKFNSKFESGNLRKVIQIRKNEYDLILNADINSNHYHQWFYFEVSGMRTGIAYRFNIINCEKSNSQFNYGMQPLMYSVQDALNGQPWWIRVGTDICYYKNHFSRSSAAAGGQKGKSYYTTTFTVSFPHKEDVCYFAYHYPYTYSTLMMHLQKLESTYNPQQIYFRHDVLCETLSGNNCPLLTITAMPESNYYEHICQFRNRPFIFLSARVHPGETNASWVMKGTLEYLMGNNPTAQALRESYIFKIVPMLNPDGVINGNHRCSLSGEDLNRQWQNPNPDLHPAIYHAKGLLQYLSAIKRLPLVYCDYHGHSRKKNVFMYGCSIKETMWHTNVSAASCDLIEDLSYRTLPKILSQTAPAFCMSSCSFVVEKSKESTARVVVWREIGVQRSYTMESTLCGCDQGKYKGLQIGTRELEEMGAKFCSALLRLKRLTSPLEYNLPPSLFDIENDLIESSCKVTSPTTYVMEEDEPRFLEEVDYSAESNDEPDVELTNNAGDYEPSLQEDGLSDSEITRTHLP

Target ORF information:

RefSeq Version	XM_015419279.1
Organism	Gekko japonicus
Definition	Gekko japonicus ATP/GTP binding protein 1 (AGTPBP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015419279.1

ORF Insert Sequence:

ATGAATAAAT TAAGATCGAC ATCAGAAAAA AGTATAACTA ACAATTCCCG GATTGCAAGT 
TTATTGACAC AACTTGAGAA GATCAATGCA GAATGTTTGG TGGAAACCGA CACCGCCAGA 
TATGTTACAT CAAAGATACT CCATCTAACT CAAAGTCAAG AAAAAATAAA GAAGGAGATG 
ACTGCAAAAG GTTCAACAGG CATTGAAGTT ATTCTCTCAA CTTTAGAGAA TACAAGAGAT 
CTTCAGACTA CTCTAAATAT CTTAAACATC CTGATTGAGT TGGTAGCAGT CGGTGGTAGC 
CGGAGAACAA GTATTCTCGT TTCCAAAGGA GGAACACAGA TCTTACTGCA ATTGCTTGTG 
AATGTCAGTA AAGATTCCCC ACCAAATGAA GACTTAATGG TGCTACTTCA TTCTCTCCTT 
GCAAAAATTG GTCCAAAAGA TAAAAAAATT GGAGTAAAAG CCAGAATTAG CGGTGCTTTA 
AATATAACAC TAAATATAGT GAAGCAGAAC TTGCAGAATC AAAGATTAAT ACTGCCTTGT 
CTTCAAGTGT TACGAGTATA TTCAACCAAC TCTGTAAATG CTGTGTCCTT GGGTAAGAAT 
GGAATTGTGG AGCTGATGTT TAAGATCATC GGTCCTTTCA GCAAAAAGAA CACTGGCCTT 
ATGAAGGTTG CTTTAGACAC TTTTGCTGCA TTGCTAAGAT CGAAAACAAA TGCTAGGAGA 
GCAGTAGACA GAGGCTATGT CCACGTGCTT TTAACAATCT ATGTAGACTG GCACCGTCAT 
GATAGCCGCC ATAGACATAT GTTGATCCGC AAAGGAATCT TGCAGTGTAT TAAAAATGTT 
ACGAACACCA AGCTGGGAAG AAAAGCATTT CTTGATGTCA GTGGGATGAA AATTCTGTAC 
AACACATCAC AAGAATGCCT TGCAGTAAGA ACTCTTGATC CATTAGTCAA CACTTCCAGT 
CTGATAATGA GAAAATGTTA TCCTAAAAAT CGTCTTCCGC TGCCAACCAT TAAAAGTGCT 
TTCCATTTCC AATTACCTGT CATCCCTGGT AATGGACCTG TGGCTCAGCT GTATAGTTTG 
CCTCCTGAAG TGGATGACGT AGTAGATGAA AGTGATGACA ATGATGACAC CGAAACAGAT 
ACTGAGATTG AAAATGGTAA TGAAGATGAC ATAGAGCAGC ATTTTAAGAA TGATGATATA 
GAGACTGATA TCAACAAGTT GAAACCAAAG CAGGAGTTGG GACGACCTAT AGAAGATCTA 
AAGATGTATG AAAATTTTTT CCCAGAACTT GTGGAGGATT TTCAGGAATA CGATTTAGTT 
TCCAAAGAAC CAAAGCCTTT TTCAACTGAT GTATCACTAG GAGGACCTAT TATTGTTCCT 
ACCGCTGGTG ATGAACAGTC TCCCACAAAT CATATCATAG AAATTCCTGT GAAGGGGAAT 
CAGCCTTTGC AAATGGAGGA CTACAATAAA AGACCCATCT CTTTGGGTCT GGTAAAGAAA 
AATAGCAACA CAGACAATAG TTTACAGCAT CAAGGTGATC ATTTGAAATT ACATTCATCG 
TGCCAGTGCC TAAACCATGA AATAGTCAAA GGCTTGGACA GAATTACTTT GCAGAACACT 
CCCAAAAATG AACAGTCTTA CAGTTCAAGA GCAACAACCC AAAAAGAGTG TAAAACTAGC 
TGTAGTGAAG TTACCTGTAG TAAACCCTTA CAACATGTTT CATCCTGTGG AAGTGTTTTG 
TTTGAGGGAC GGTCTGTTCA GCTGGGAAAG CTGTGCTGTT CTGGTGCTGA TCCTGAAGAG 
GAGGAAGTAC CTTGCTCAGT TTCTTTGGGA GAGCAAGTGA TGCTTGATGT GCCTGATCTA 
TTACAACTCC ATGACCCAGA ACTTTATATT GAGATAGTGA AAAATACAAA GTCTGTTCCT 
GAATATTCAG AAGTGGCTTA TCCTGACTAT TTTGGACACT TGCCACCGCC ATTTAAAGAG 
CCCATTCTTG AAAGGCCGTA TGGTGTTCAA CGGACAAAAA TTGCCCAGGA TATTGAAAGG 
CTAATTCATC CTAATGATAT TATAGACAGA GTTGTGTATG ACCTTGACAG TCCCAGCTAC 
ATGGTGCCAG ATGAAGGAGA CATTTTGAAG TTTAATTCTA AATTTGAATC AGGGAACTTG 
CGCAAAGTCA TTCAGATCAG AAAAAATGAG TATGATCTTA TCCTGAATGC TGATATAAAC 
AGCAACCATT ATCACCAGTG GTTTTACTTC GAAGTCAGTG GAATGAGGAC AGGCATTGCT 
TACCGATTTA ATATAATCAA CTGTGAAAAG TCAAACAGTC AGTTCAATTA TGGTATGCAG 
CCCCTCATGT ACTCTGTTCA AGATGCATTA AATGGCCAAC CCTGGTGGAT TCGAGTAGGA 
ACAGATATTT GTTACTACAA GAATCATTTT TCTAGAAGCT CAGCTGCTGC TGGAGGTCAG 
AAGGGAAAAT CCTACTATAC AACTACCTTC ACTGTTAGCT TCCCACACAA GGAAGATGTT 
TGCTACTTCG CTTACCATTA TCCATATACT TATTCAACTT TAATGATGCA CCTTCAAAAA 
TTGGAATCTA CATACAATCC TCAGCAGATA TATTTCCGAC ACGATGTTTT GTGTGAGACC 
CTTTCTGGAA ACAACTGTCC TTTACTCACT ATAACAGCCA TGCCAGAATC AAATTACTAT 
GAACATATCT GTCAGTTCAG AAACCGTCCT TTTATATTCT TATCTGCTCG TGTACATCCT 
GGAGAAACTA ATGCAAGCTG GGTTATGAAG GGAACACTGG AATATCTCAT GGGTAATAAT 
CCCACTGCCC AGGCATTACG AGAATCTTAT ATTTTTAAAA TTGTTCCAAT GCTTAACCCA 
GATGGGGTCA TCAATGGAAA CCATCGCTGT TCTTTAAGTG GGGAGGACTT GAATAGACAA 
TGGCAAAACC CAAACCCAGA TCTGCATCCT GCAATTTATC ATGCCAAGGG TCTCCTCCAG 
TACCTGTCTG CTATAAAGCG TTTGCCACTG GTTTACTGTG ATTATCATGG TCATTCTCGC 
AAGAAGAATG TGTTCATGTA TGGCTGCAGT ATCAAGGAAA CAATGTGGCA CACAAATGTT 
AGTGCAGCTT CTTGTGATCT GATTGAAGAC TTAAGTTACA GGACACTCCC TAAGATTCTG 
AGCCAGACAG CTCCAGCATT CTGCATGAGC AGCTGCAGTT TTGTGGTGGA AAAGTCTAAA 
GAATCTACAG CACGTGTTGT AGTCTGGAGA GAGATAGGTG TTCAGAGAAG TTATACCATG 
GAAAGCACAT TGTGTGGATG TGATCAAGGG AAATACAAGG GTTTGCAGAT TGGGACACGT 
GAGCTTGAAG AGATGGGAGC TAAATTCTGT TCTGCTTTAC TGCGTTTGAA AAGATTGACA 
TCGCCACTGG AATACAACCT ACCACCTAGC CTCTTTGACA TTGAAAATGA TTTGATTGAG 
TCCAGTTGTA AAGTAACAAG CCCCACTACA TATGTAATGG AAGAAGATGA GCCTCGCTTC 
CTTGAAGAAG TGGATTATAG TGCTGAAAGC AATGATGAGC CAGATGTTGA ATTGACAAAT 
AATGCTGGTG ACTATGAGCC CAGTCTTCAA GAAGATGGAC TTTCTGACTC TGAAATTACA 
AGGACACATT TGCCTTAA