WLS cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	WLS
Entrez Gene ID	107114350
Full Name	wntless Wnt ligand secretion mediator
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015415793.1	XP_015271279.1	protein wntless homolog

Pathways from BioSystems
Gene Ontology

The following WLS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WLS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe26100	XM_015415793.1 Latest version!	Gekko japonicus wntless Wnt ligand secretion mediator (WLS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WLS ( XM_015415793.1 ) cDNA ORF clone, Gekko japonicus -> XP_015271279.1 Gekko japonicus wntless Wnt ligand secretion mediator (WLS), mRNA.

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CloneID	OGe26100
Clone ID Related Accession (Same CDS sequence)	XM_015415793.1
Accession Version	XM_015415793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	protein wntless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015169172.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG CAATAATCGA GAACATGAGC ACGAAAAAGC TCTGCATTGT TGGAGGGATC 
TTGCTGGTTT TCCAAGTCAT CGCTTTTCTA GTGGGAGGTC TGATCGCTCC AAGTCCCACC 
ACAGCTGTGT CCTATCTGGC AAACAAATGT GTAGATGTAG AGAAGAACCA TCAGAAGACC 
AAATGGTATT TGTCCTGGGG GTCCAATCCA TGTGAAAAAA TCAGGGACTT TGATGAAGCA 
GTGAGCAGGC AGATAGAAGC CAATGACATT GTGTTCTCCA TTCATGCGCC TCATCCTCAC 
AATGAGATGA GCCCTTGGTT CCAGTTCATG CTCTACGTTT TGCACGTGGA CATTGCCTTC 
AAGATGGACA ACCAAATTAA AAACAATGCA GAGATTACAT TTAATGTGTC CTTGGGATAC 
CGTGACGACA TGGTCAGTGA GTGGCAACCA ATGGCATATG CAGAAGAGAC TCGGAAGCTC 
AAATGTGCCT TTAACTATCC AAAAACTAAA GAAAATGAAG ACAGTTTCTA TGATTGTGAT 
GTACTTTCAT TCATGGAAAT TGGAACTGTT GCTCACAAAT TCTACCTTGT CAATATTCGG 
CTCCCTGTGC ATGAAAAGAA AGGTATCAAT GTAGGAATTG GAGATATAAG AGATATTCGT 
CTAGTGGGCA TCCATCAAAA TGGAGGCTTT ACAAAGGTGT GGTTTGCAAT GAAAACAGTC 
CTCACACCGA GCATCTTCAC CATCATGGTG TGGTACTGGA GACGGATCAC AATGATGACG 
CGGCCACCTG TCTTACTGGA AAAAGTTATC TTTGCTCTTG GGATTTCCAT GACATTCATA 
AACATCCCAG TGGAGTGGTT TTCTATTGGG TTTGAGTGGA CTTGGATGTT GCTGTTTGGA 
GATATTCGCC AAGGAATCTT CTATGCTATG CTTCTGTCAT TTTGGATCAT CTTCTGTGGA 
GAGCATATGA TGGATCAGAA TGAACGAAAT CGTTTCTCTA GCTACTGGAA ACAAGTCGGG 
CCAATTGCTG TAGGCGCCTT CTGTCTGTTT GTCTTTGACA TGTGTGAGAG AGGGGTACAA 
TTAAAAAATC CATTCTACAG TATCTGGACC ACAGATGTTG GTACGGAACT CGCTATGGCA 
TTTATTATCG TTGCTGGGAT CTGCCTCTGT CTCTACTTCC TGTTCCTGTG TTTTATGGTG 
TTTCAAGTGT TCCGAAACAT CAGTGGTAAG CAATCCAGCC TCCCAGCAAT GAGTAAAGCT 
CGCAGACTTC ACTATGAGGG GCTAATTTTT AGGTTCAAGT TCTTGATGCT GATCACACTG 
GCTTGTGCTG CCATGACTGT AATATTTTTC ATTGTCAGCC AGGTGACTGA AGGCCATTGG 
AAATGGGGTG GCTACACGGT TGAACTGAAC AGTGCCTTTT TCACAGGAAT TTACGGAATG 
TGGAACCTTT ATGTCTTTGC CCTCATGTTC CTGTATGCAC CTTCACACAA AAACTATGGT 
GAAGATCAGT CAATTGGTGA CCTGGGAATA AGCAGCGGAG AAGAACTTCA GCTCACGACC 
ACCATCACCC ATGTTGATGG ACCTACAGAG GTTTACAAAC TGGCTCGCAA GGAGGCCCAA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015271279.1
CDS	107..1732
Translation	MAGAIIENMSTKKLCIVGGILLVFQVIAFLVGGLIAPSPTTAVSYLANKCVDVEKNHQKTKWYLSWGSNPCEKIRDFDEAVSRQIEANDIVFSIHAPHPHNEMSPWFQFMLYVLHVDIAFKMDNQIKNNAEITFNVSLGYRDDMVSEWQPMAYAEETRKLKCAFNYPKTKENEDSFYDCDVLSFMEIGTVAHKFYLVNIRLPVHEKKGINVGIGDIRDIRLVGIHQNGGFTKVWFAMKTVLTPSIFTIMVWYWRRITMMTRPPVLLEKVIFALGISMTFINIPVEWFSIGFEWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQNERNRFSSYWKQVGPIAVGAFCLFVFDMCERGVQLKNPFYSIWTTDVGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKARRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGYTVELNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSIGDLGISSGEELQLTTTITHVDGPTEVYKLARKEAQE

Target ORF information:

RefSeq Version	XM_015415793.1
Organism	Gekko japonicus
Definition	Gekko japonicus wntless Wnt ligand secretion mediator (WLS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015415793.1

ORF Insert Sequence:

ATGGCTGGGG CAATAATCGA GAACATGAGC ACGAAAAAGC TCTGCATTGT TGGAGGGATC 
TTGCTGGTTT TCCAAGTCAT CGCTTTTCTA GTGGGAGGTC TGATCGCTCC AAGTCCCACC 
ACAGCTGTGT CCTATCTGGC AAACAAATGT GTAGATGTAG AGAAGAACCA TCAGAAGACC 
AAATGGTATT TGTCCTGGGG GTCCAATCCA TGTGAAAAAA TCAGGGACTT TGATGAAGCA 
GTGAGCAGGC AGATAGAAGC CAATGACATT GTGTTCTCCA TTCATGCGCC TCATCCTCAC 
AATGAGATGA GCCCTTGGTT CCAGTTCATG CTCTACGTTT TGCACGTGGA CATTGCCTTC 
AAGATGGACA ACCAAATTAA AAACAATGCA GAGATTACAT TTAATGTGTC CTTGGGATAC 
CGTGACGACA TGGTCAGTGA GTGGCAACCA ATGGCATATG CAGAAGAGAC TCGGAAGCTC 
AAATGTGCCT TTAACTATCC AAAAACTAAA GAAAATGAAG ACAGTTTCTA TGATTGTGAT 
GTACTTTCAT TCATGGAAAT TGGAACTGTT GCTCACAAAT TCTACCTTGT CAATATTCGG 
CTCCCTGTGC ATGAAAAGAA AGGTATCAAT GTAGGAATTG GAGATATAAG AGATATTCGT 
CTAGTGGGCA TCCATCAAAA TGGAGGCTTT ACAAAGGTGT GGTTTGCAAT GAAAACAGTC 
CTCACACCGA GCATCTTCAC CATCATGGTG TGGTACTGGA GACGGATCAC AATGATGACG 
CGGCCACCTG TCTTACTGGA AAAAGTTATC TTTGCTCTTG GGATTTCCAT GACATTCATA 
AACATCCCAG TGGAGTGGTT TTCTATTGGG TTTGAGTGGA CTTGGATGTT GCTGTTTGGA 
GATATTCGCC AAGGAATCTT CTATGCTATG CTTCTGTCAT TTTGGATCAT CTTCTGTGGA 
GAGCATATGA TGGATCAGAA TGAACGAAAT CGTTTCTCTA GCTACTGGAA ACAAGTCGGG 
CCAATTGCTG TAGGCGCCTT CTGTCTGTTT GTCTTTGACA TGTGTGAGAG AGGGGTACAA 
TTAAAAAATC CATTCTACAG TATCTGGACC ACAGATGTTG GTACGGAACT CGCTATGGCA 
TTTATTATCG TTGCTGGGAT CTGCCTCTGT CTCTACTTCC TGTTCCTGTG TTTTATGGTG 
TTTCAAGTGT TCCGAAACAT CAGTGGTAAG CAATCCAGCC TCCCAGCAAT GAGTAAAGCT 
CGCAGACTTC ACTATGAGGG GCTAATTTTT AGGTTCAAGT TCTTGATGCT GATCACACTG 
GCTTGTGCTG CCATGACTGT AATATTTTTC ATTGTCAGCC AGGTGACTGA AGGCCATTGG 
AAATGGGGTG GCTACACGGT TGAACTGAAC AGTGCCTTTT TCACAGGAAT TTACGGAATG 
TGGAACCTTT ATGTCTTTGC CCTCATGTTC CTGTATGCAC CTTCACACAA AAACTATGGT 
GAAGATCAGT CAATTGGTGA CCTGGGAATA AGCAGCGGAG AAGAACTTCA GCTCACGACC 
ACCATCACCC ATGTTGATGG ACCTACAGAG GTTTACAAAC TGGCTCGCAA GGAGGCCCAA 
GAGTAA