UBA1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA1
Entrez Gene ID	107106087
Full Name	ubiquitin like modifier activating enzyme 1
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015406176.1	XP_015261662.1	ubiquitin-like modifier-activating enzyme 1
XM_015406175.1	XP_015261661.1	ubiquitin-like modifier-activating enzyme 1

Pathways from BioSystems
Gene Ontology

The following UBA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe18799	XM_015406175.1 Latest version!	Gekko japonicus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGe18799	XM_015406176.1 Latest version!	Gekko japonicus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA1 ( XM_015406176.1 ) cDNA ORF clone, Gekko japonicus -> XP_015261662.1 Gekko japonicus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGe18799
Clone ID Related Accession (Same CDS sequence)	XM_015406176.1 , XM_015406175.1
Accession Version	XM_015406176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015162512.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGT CGCTTGTCTG AATCTGAGAC GAAGTCGGGT 
TCAAACTGCT CCTCTGGCAA ATTGGTACAG ACTGACCTAC GCGAAGCGCC TGCCAACGGT 
ATGGCCAAGA ATGGAAGCGA AGCAGACATT GATGAGGGTC TGTACTCGAG ACAGCTGTAT 
GTGCTGGGCC ATGAGGCCAT GAAACGGATG CAGAATGCCA ACATTCTGGT CTCTGGGCTG 
CGAGGACTGG GCGTGGAGAT CGCCAAGAAC ATCATCTTGG GCGGAGTCAA ATCCGTCACA 
GTCCATGACC AGGGGGTGGC TGAGTGGTCG GACCTTTCCT CCCAGTTCTA TCTGCATGAG 
GAGGACCTGG GGAAAAATCG GGCGGAAGTA TCCCAGCCAC GACTGGCAGA GCTCAACTCA 
TACGTACCCG TTACGGCTTA CACTGGACCC CTGAGCGAGG ACTTCCTGGG CACCTTCCAG 
GTGGTCGTGC TCACCAATTG CCCCCTGGAA GAGCAGCTGC GCATTAGTGA CTTCTGTCAC 
AGCCAAGACA TCAAACTGGT AGTGGCGGAT ACCAAGGGAC TGTTTGGCCA GTTGTTTTGT 
GACTTTGGTG ATGAGATGGT TGTGACGGAC ACCAACGGGG AACAGCCGCT CAGTGCTATG 
GTCTCCATGG TCACCAAGGG ATGCCCCGGA GAGGTAACCT GCCTAGATGA AGCCCGGCAT 
GGCTTTGAGA GCGGGGACTT TGTTTCCTTC ACTGAAGTGG AGGGCATGAC ACAACTCAAC 
AGCAGTGAAC CCATGGAAAT CAAAGTGCTC GGCCCCTACA CATTCAGCAT TGGTGATACC 
TCCAGCTTTT CGGACTATGT ACGCGGTGGC ATCGTCACCC AGGTTAAGAT GCCAAAGAAG 
ATTAGCTTTA AATCTCTACG TCGTTCCCTG CAAGAGCCAG AATTCATACT CACCGACTTT 
GGCAAGTTTG ATAGGCCAGC CCAGCTGCAC TTTGGGTTCC AGGCTTTGCA TGAATTCCAC 
AAGAAGCATG GCCATTTCCC CAAGCCCCGT AACCAGGCAG ACGCTGCTGA GGTGCTGACG 
TTGACCAAGG AACTGAATGA ACAGGCAGCA CCCCCCCTTA AGCAAGAGCA GCTGAATGAG 
GACGTCGTCA AAGAACTGGC CTTCCAGGCT ACCGGGGATC TGGCCCCCAT CAATGCCTTT 
GTCGGAGGAC TGGCTGCACA GGAAGTCATG AAGGCGTGTT CGGGGAAATT CATGCCCATC 
GCTCAGTGGC TATACTTTGA TGCCCTGGAG TGCCTGCCAG AGGAAAACAA AGACGCCCTA 
CTGACGGAGG AGAACTGCAC CCCTAAACAC TGCCGATACG ATGGACAGAT TGCCGTCTTC 
GGCAGCGACC TGCAGGCTAA GCTGGGTCAG CAGAAGTACT TCCTGGTTGG TGCCGGTGCC 
ATTGGCTGCG AGCTGCTGAA GAACTTTGCC ATGATTGGTC TGGGCTGCGG TCAAGGCGGA 
GAAATAGTTG TGACGGACAT GGACACCATT GAGAAATCCA ACCTCAACAG GCAGTTCCTA 
TTCCGGCCTT GGGATGTGAC GAAAATGAAA TCAGATACAG CGGCTGCCTC TGTGAAAGAA 
ATGAACCCAC ACATCAACAT TGTGAGCCAC CAAGACCGCG TGGGCCCCGA CACTGAACGT 
GTTTATGATG ATGACTTCTT TGAGAACCTT GATGGAGTGG CAAATGCGTT GGACAATGTA 
GATGCCCGGA TGTACATGGA CCGTCGCTGT GTCTACTACC GCAAGCCCCT GCTGGAATCG 
GGGACGCTGG GGACCAAAGG GAACATCCAG GTGGTGATCC CCTTCCTGAC AGAGTCCTAC 
AGCTCCAGCC AGGACCCGCC CGAAAAGTCC ATCCCCATCT GCACTCTGAA GAACTTTCCC 
AATGCCATCG AGCACACCCT GCAGTGGGCC CGTGATGAAT TTGAAGGTCT TTTCAAGCAG 
CCTGCAGAAA ATGTCAACCA GTACATCACA GACGCCAAGT TCATGGAACG GACCCAGAAA 
CTGCCTGGCA CCCAACCTCT GGAGGTGTTA GAAGCTGTCT ACAAGAGCTT GGTGACTGAC 
CGGCCTAAGA CGTGGGCGGA CTGCATAGCC TGGGCATGCA ATCATTGGCA TACCCAGTAC 
TGCAACAACA TTCGTCAGCT GCTGCACAAT TTCCCTCCAA ACCAGAAAAC CAACTCGGGA 
ACCCTCTTCT GGTCGGGTCC CAAGCGATGC CCTCACGCCC TCACCTTCGA TACCAACAAT 
CCCTTGCACA TGGATTATGT GGTGGCAGGT GCGAACTTGT TTGCACAGAC CTACGGCATC 
ACAGGCACCA GGGACAGGGC GGCCATCGCG GAGCTGCTGC GCCAGGTCCA GGTCCCAGAA 
TTCACTCCCA AGTCGGGGGT GCGCATCCAC ATCTCCGACC AAGAGCTGCA GAACGCCAAC 
GCTTCAGTCG ACGACAGCCG GCTGGAGGAA CTGAAATCTT CACTCCCAAG TCCCCAGCAA 
CTGCAAGACT TCAGGATGTT TCCGGTCGAT TTTGAAAAGG ATGATGACAC GAACTTCCAC 
ATGGATTTCA TTGTGGCTGC GTCCAACCTG CGGGCAGAGA ACTACGATAT TCCTCCTGCT 
GACCGGCATA AGAGCAAGTT GATCGCCGGG AAGATTATAC CCGCCATCGC CACGACGACA 
GCAGCTGTAG TAGGCTTGGT GTGTCTGGAA CTCTACAAGG TGATACAGGG CCACCAGCGA 
CTTGAGTCCT TCAAGAATGG CTTCCTCAAC TTGGCGTTGC CTTTCTTTGC TTTCTCTGAG 
CCCGTTGCCT GCCCCAAGAA CAAGTATTAT AGCACTGAAT GGACTCTGTG GGACCGTTTT 
GAAGTGCAGG GCATCCAGCC CGACGGGCAG GAGATGACGC TGCGCGAATT CCTGGCCTAC 
TTCAAGAAAG AGCACAAGTT GGAGATTACG ATGTTGTCCC AGGGAGTCTC CATGCTGTAC 
TCGTTCTTCA TGCAGCCGGC GAAGCTGAAG GAGCGCCACG ACCAGCCGAT GACGGAGATT 
GTCACCAGAG TCTCCAAGAA GAAGATCGGC CGCCACGTGA AGGCCTTGGT CTTTGAGCTG 
TGCTGTAACG ACGACAGTGG GGACGACACG GAGGTGCCCT ACGTGCGCTA CACCATCCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015261662.1
CDS	106..3288
Translation	MSSSPLSKKRRLSESETKSGSNCSSGKLVQTDLREAPANGMAKNGSEADIDEGLYSRQLYVLGHEAMKRMQNANILVSGLRGLGVEIAKNIILGGVKSVTVHDQGVAEWSDLSSQFYLHEEDLGKNRAEVSQPRLAELNSYVPVTAYTGPLSEDFLGTFQVVVLTNCPLEEQLRISDFCHSQDIKLVVADTKGLFGQLFCDFGDEMVVTDTNGEQPLSAMVSMVTKGCPGEVTCLDEARHGFESGDFVSFTEVEGMTQLNSSEPMEIKVLGPYTFSIGDTSSFSDYVRGGIVTQVKMPKKISFKSLRRSLQEPEFILTDFGKFDRPAQLHFGFQALHEFHKKHGHFPKPRNQADAAEVLTLTKELNEQAAPPLKQEQLNEDVVKELAFQATGDLAPINAFVGGLAAQEVMKACSGKFMPIAQWLYFDALECLPEENKDALLTEENCTPKHCRYDGQIAVFGSDLQAKLGQQKYFLVGAGAIGCELLKNFAMIGLGCGQGGEIVVTDMDTIEKSNLNRQFLFRPWDVTKMKSDTAAASVKEMNPHINIVSHQDRVGPDTERVYDDDFFENLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNIQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYITDAKFMERTQKLPGTQPLEVLEAVYKSLVTDRPKTWADCIAWACNHWHTQYCNNIRQLLHNFPPNQKTNSGTLFWSGPKRCPHALTFDTNNPLHMDYVVAGANLFAQTYGITGTRDRAAIAELLRQVQVPEFTPKSGVRIHISDQELQNANASVDDSRLEELKSSLPSPQQLQDFRMFPVDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVIQGHQRLESFKNGFLNLALPFFAFSEPVACPKNKYYSTEWTLWDRFEVQGIQPDGQEMTLREFLAYFKKEHKLEITMLSQGVSMLYSFFMQPAKLKERHDQPMTEIVTRVSKKKIGRHVKALVFELCCNDDSGDDTEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_015406176.1
Organism	Gekko japonicus
Definition	Gekko japonicus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015406176.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGT CGCTTGTCTG AATCTGAGAC GAAGTCGGGT 
TCAAACTGCT CCTCTGGCAA ATTGGTACAG ACTGACCTAC GCGAAGCGCC TGCCAACGGT 
ATGGCCAAGA ATGGAAGCGA AGCAGACATT GATGAGGGTC TGTACTCGAG ACAGCTGTAT 
GTGCTGGGCC ATGAGGCCAT GAAACGGATG CAGAATGCCA ACATTCTGGT CTCTGGGCTG 
CGAGGACTGG GCGTGGAGAT CGCCAAGAAC ATCATCTTGG GCGGAGTCAA ATCCGTCACA 
GTCCATGACC AGGGGGTGGC TGAGTGGTCG GACCTTTCCT CCCAGTTCTA TCTGCATGAG 
GAGGACCTGG GGAAAAATCG GGCGGAAGTA TCCCAGCCAC GACTGGCAGA GCTCAACTCA 
TACGTACCCG TTACGGCTTA CACTGGACCC CTGAGCGAGG ACTTCCTGGG CACCTTCCAG 
GTGGTCGTGC TCACCAATTG CCCCCTGGAA GAGCAGCTGC GCATTAGTGA CTTCTGTCAC 
AGCCAAGACA TCAAACTGGT AGTGGCGGAT ACCAAGGGAC TGTTTGGCCA GTTGTTTTGT 
GACTTTGGTG ATGAGATGGT TGTGACGGAC ACCAACGGGG AACAGCCGCT CAGTGCTATG 
GTCTCCATGG TCACCAAGGG ATGCCCCGGA GAGGTAACCT GCCTAGATGA AGCCCGGCAT 
GGCTTTGAGA GCGGGGACTT TGTTTCCTTC ACTGAAGTGG AGGGCATGAC ACAACTCAAC 
AGCAGTGAAC CCATGGAAAT CAAAGTGCTC GGCCCCTACA CATTCAGCAT TGGTGATACC 
TCCAGCTTTT CGGACTATGT ACGCGGTGGC ATCGTCACCC AGGTTAAGAT GCCAAAGAAG 
ATTAGCTTTA AATCTCTACG TCGTTCCCTG CAAGAGCCAG AATTCATACT CACCGACTTT 
GGCAAGTTTG ATAGGCCAGC CCAGCTGCAC TTTGGGTTCC AGGCTTTGCA TGAATTCCAC 
AAGAAGCATG GCCATTTCCC CAAGCCCCGT AACCAGGCAG ACGCTGCTGA GGTGCTGACG 
TTGACCAAGG AACTGAATGA ACAGGCAGCA CCCCCCCTTA AGCAAGAGCA GCTGAATGAG 
GACGTCGTCA AAGAACTGGC CTTCCAGGCT ACCGGGGATC TGGCCCCCAT CAATGCCTTT 
GTCGGAGGAC TGGCTGCACA GGAAGTCATG AAGGCGTGTT CGGGGAAATT CATGCCCATC 
GCTCAGTGGC TATACTTTGA TGCCCTGGAG TGCCTGCCAG AGGAAAACAA AGACGCCCTA 
CTGACGGAGG AGAACTGCAC CCCTAAACAC TGCCGATACG ATGGACAGAT TGCCGTCTTC 
GGCAGCGACC TGCAGGCTAA GCTGGGTCAG CAGAAGTACT TCCTGGTTGG TGCCGGTGCC 
ATTGGCTGCG AGCTGCTGAA GAACTTTGCC ATGATTGGTC TGGGCTGCGG TCAAGGCGGA 
GAAATAGTTG TGACGGACAT GGACACCATT GAGAAATCCA ACCTCAACAG GCAGTTCCTA 
TTCCGGCCTT GGGATGTGAC GAAAATGAAA TCAGATACAG CGGCTGCCTC TGTGAAAGAA 
ATGAACCCAC ACATCAACAT TGTGAGCCAC CAAGACCGCG TGGGCCCCGA CACTGAACGT 
GTTTATGATG ATGACTTCTT TGAGAACCTT GATGGAGTGG CAAATGCGTT GGACAATGTA 
GATGCCCGGA TGTACATGGA CCGTCGCTGT GTCTACTACC GCAAGCCCCT GCTGGAATCG 
GGGACGCTGG GGACCAAAGG GAACATCCAG GTGGTGATCC CCTTCCTGAC AGAGTCCTAC 
AGCTCCAGCC AGGACCCGCC CGAAAAGTCC ATCCCCATCT GCACTCTGAA GAACTTTCCC 
AATGCCATCG AGCACACCCT GCAGTGGGCC CGTGATGAAT TTGAAGGTCT TTTCAAGCAG 
CCTGCAGAAA ATGTCAACCA GTACATCACA GACGCCAAGT TCATGGAACG GACCCAGAAA 
CTGCCTGGCA CCCAACCTCT GGAGGTGTTA GAAGCTGTCT ACAAGAGCTT GGTGACTGAC 
CGGCCTAAGA CGTGGGCGGA CTGCATAGCC TGGGCATGCA ATCATTGGCA TACCCAGTAC 
TGCAACAACA TTCGTCAGCT GCTGCACAAT TTCCCTCCAA ACCAGAAAAC CAACTCGGGA 
ACCCTCTTCT GGTCGGGTCC CAAGCGATGC CCTCACGCCC TCACCTTCGA TACCAACAAT 
CCCTTGCACA TGGATTATGT GGTGGCAGGT GCGAACTTGT TTGCACAGAC CTACGGCATC 
ACAGGCACCA GGGACAGGGC GGCCATCGCG GAGCTGCTGC GCCAGGTCCA GGTCCCAGAA 
TTCACTCCCA AGTCGGGGGT GCGCATCCAC ATCTCCGACC AAGAGCTGCA GAACGCCAAC 
GCTTCAGTCG ACGACAGCCG GCTGGAGGAA CTGAAATCTT CACTCCCAAG TCCCCAGCAA 
CTGCAAGACT TCAGGATGTT TCCGGTCGAT TTTGAAAAGG ATGATGACAC GAACTTCCAC 
ATGGATTTCA TTGTGGCTGC GTCCAACCTG CGGGCAGAGA ACTACGATAT TCCTCCTGCT 
GACCGGCATA AGAGCAAGTT GATCGCCGGG AAGATTATAC CCGCCATCGC CACGACGACA 
GCAGCTGTAG TAGGCTTGGT GTGTCTGGAA CTCTACAAGG TGATACAGGG CCACCAGCGA 
CTTGAGTCCT TCAAGAATGG CTTCCTCAAC TTGGCGTTGC CTTTCTTTGC TTTCTCTGAG 
CCCGTTGCCT GCCCCAAGAA CAAGTATTAT AGCACTGAAT GGACTCTGTG GGACCGTTTT 
GAAGTGCAGG GCATCCAGCC CGACGGGCAG GAGATGACGC TGCGCGAATT CCTGGCCTAC 
TTCAAGAAAG AGCACAAGTT GGAGATTACG ATGTTGTCCC AGGGAGTCTC CATGCTGTAC 
TCGTTCTTCA TGCAGCCGGC GAAGCTGAAG GAGCGCCACG ACCAGCCGAT GACGGAGATT 
GTCACCAGAG TCTCCAAGAA GAAGATCGGC CGCCACGTGA AGGCCTTGGT CTTTGAGCTG 
TGCTGTAACG ACGACAGTGG GGACGACACG GAGGTGCCCT ACGTGCGCTA CACCATCCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBA1 ( XM_015406175.1 ) cDNA ORF clone, Gekko japonicus -> XP_015261661.1 Gekko japonicus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGe18799
Clone ID Related Accession (Same CDS sequence)	XM_015406176.1 , XM_015406175.1
Accession Version	XM_015406175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	ubiquitin-like modifier-activating enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015162512.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGT CGCTTGTCTG AATCTGAGAC GAAGTCGGGT 
TCAAACTGCT CCTCTGGCAA ATTGGTACAG ACTGACCTAC GCGAAGCGCC TGCCAACGGT 
ATGGCCAAGA ATGGAAGCGA AGCAGACATT GATGAGGGTC TGTACTCGAG ACAGCTGTAT 
GTGCTGGGCC ATGAGGCCAT GAAACGGATG CAGAATGCCA ACATTCTGGT CTCTGGGCTG 
CGAGGACTGG GCGTGGAGAT CGCCAAGAAC ATCATCTTGG GCGGAGTCAA ATCCGTCACA 
GTCCATGACC AGGGGGTGGC TGAGTGGTCG GACCTTTCCT CCCAGTTCTA TCTGCATGAG 
GAGGACCTGG GGAAAAATCG GGCGGAAGTA TCCCAGCCAC GACTGGCAGA GCTCAACTCA 
TACGTACCCG TTACGGCTTA CACTGGACCC CTGAGCGAGG ACTTCCTGGG CACCTTCCAG 
GTGGTCGTGC TCACCAATTG CCCCCTGGAA GAGCAGCTGC GCATTAGTGA CTTCTGTCAC 
AGCCAAGACA TCAAACTGGT AGTGGCGGAT ACCAAGGGAC TGTTTGGCCA GTTGTTTTGT 
GACTTTGGTG ATGAGATGGT TGTGACGGAC ACCAACGGGG AACAGCCGCT CAGTGCTATG 
GTCTCCATGG TCACCAAGGG ATGCCCCGGA GAGGTAACCT GCCTAGATGA AGCCCGGCAT 
GGCTTTGAGA GCGGGGACTT TGTTTCCTTC ACTGAAGTGG AGGGCATGAC ACAACTCAAC 
AGCAGTGAAC CCATGGAAAT CAAAGTGCTC GGCCCCTACA CATTCAGCAT TGGTGATACC 
TCCAGCTTTT CGGACTATGT ACGCGGTGGC ATCGTCACCC AGGTTAAGAT GCCAAAGAAG 
ATTAGCTTTA AATCTCTACG TCGTTCCCTG CAAGAGCCAG AATTCATACT CACCGACTTT 
GGCAAGTTTG ATAGGCCAGC CCAGCTGCAC TTTGGGTTCC AGGCTTTGCA TGAATTCCAC 
AAGAAGCATG GCCATTTCCC CAAGCCCCGT AACCAGGCAG ACGCTGCTGA GGTGCTGACG 
TTGACCAAGG AACTGAATGA ACAGGCAGCA CCCCCCCTTA AGCAAGAGCA GCTGAATGAG 
GACGTCGTCA AAGAACTGGC CTTCCAGGCT ACCGGGGATC TGGCCCCCAT CAATGCCTTT 
GTCGGAGGAC TGGCTGCACA GGAAGTCATG AAGGCGTGTT CGGGGAAATT CATGCCCATC 
GCTCAGTGGC TATACTTTGA TGCCCTGGAG TGCCTGCCAG AGGAAAACAA AGACGCCCTA 
CTGACGGAGG AGAACTGCAC CCCTAAACAC TGCCGATACG ATGGACAGAT TGCCGTCTTC 
GGCAGCGACC TGCAGGCTAA GCTGGGTCAG CAGAAGTACT TCCTGGTTGG TGCCGGTGCC 
ATTGGCTGCG AGCTGCTGAA GAACTTTGCC ATGATTGGTC TGGGCTGCGG TCAAGGCGGA 
GAAATAGTTG TGACGGACAT GGACACCATT GAGAAATCCA ACCTCAACAG GCAGTTCCTA 
TTCCGGCCTT GGGATGTGAC GAAAATGAAA TCAGATACAG CGGCTGCCTC TGTGAAAGAA 
ATGAACCCAC ACATCAACAT TGTGAGCCAC CAAGACCGCG TGGGCCCCGA CACTGAACGT 
GTTTATGATG ATGACTTCTT TGAGAACCTT GATGGAGTGG CAAATGCGTT GGACAATGTA 
GATGCCCGGA TGTACATGGA CCGTCGCTGT GTCTACTACC GCAAGCCCCT GCTGGAATCG 
GGGACGCTGG GGACCAAAGG GAACATCCAG GTGGTGATCC CCTTCCTGAC AGAGTCCTAC 
AGCTCCAGCC AGGACCCGCC CGAAAAGTCC ATCCCCATCT GCACTCTGAA GAACTTTCCC 
AATGCCATCG AGCACACCCT GCAGTGGGCC CGTGATGAAT TTGAAGGTCT TTTCAAGCAG 
CCTGCAGAAA ATGTCAACCA GTACATCACA GACGCCAAGT TCATGGAACG GACCCAGAAA 
CTGCCTGGCA CCCAACCTCT GGAGGTGTTA GAAGCTGTCT ACAAGAGCTT GGTGACTGAC 
CGGCCTAAGA CGTGGGCGGA CTGCATAGCC TGGGCATGCA ATCATTGGCA TACCCAGTAC 
TGCAACAACA TTCGTCAGCT GCTGCACAAT TTCCCTCCAA ACCAGAAAAC CAACTCGGGA 
ACCCTCTTCT GGTCGGGTCC CAAGCGATGC CCTCACGCCC TCACCTTCGA TACCAACAAT 
CCCTTGCACA TGGATTATGT GGTGGCAGGT GCGAACTTGT TTGCACAGAC CTACGGCATC 
ACAGGCACCA GGGACAGGGC GGCCATCGCG GAGCTGCTGC GCCAGGTCCA GGTCCCAGAA 
TTCACTCCCA AGTCGGGGGT GCGCATCCAC ATCTCCGACC AAGAGCTGCA GAACGCCAAC 
GCTTCAGTCG ACGACAGCCG GCTGGAGGAA CTGAAATCTT CACTCCCAAG TCCCCAGCAA 
CTGCAAGACT TCAGGATGTT TCCGGTCGAT TTTGAAAAGG ATGATGACAC GAACTTCCAC 
ATGGATTTCA TTGTGGCTGC GTCCAACCTG CGGGCAGAGA ACTACGATAT TCCTCCTGCT 
GACCGGCATA AGAGCAAGTT GATCGCCGGG AAGATTATAC CCGCCATCGC CACGACGACA 
GCAGCTGTAG TAGGCTTGGT GTGTCTGGAA CTCTACAAGG TGATACAGGG CCACCAGCGA 
CTTGAGTCCT TCAAGAATGG CTTCCTCAAC TTGGCGTTGC CTTTCTTTGC TTTCTCTGAG 
CCCGTTGCCT GCCCCAAGAA CAAGTATTAT AGCACTGAAT GGACTCTGTG GGACCGTTTT 
GAAGTGCAGG GCATCCAGCC CGACGGGCAG GAGATGACGC TGCGCGAATT CCTGGCCTAC 
TTCAAGAAAG AGCACAAGTT GGAGATTACG ATGTTGTCCC AGGGAGTCTC CATGCTGTAC 
TCGTTCTTCA TGCAGCCGGC GAAGCTGAAG GAGCGCCACG ACCAGCCGAT GACGGAGATT 
GTCACCAGAG TCTCCAAGAA GAAGATCGGC CGCCACGTGA AGGCCTTGGT CTTTGAGCTG 
TGCTGTAACG ACGACAGTGG GGACGACACG GAGGTGCCCT ACGTGCGCTA CACCATCCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015261661.1
CDS	67..3249
Translation	MSSSPLSKKRRLSESETKSGSNCSSGKLVQTDLREAPANGMAKNGSEADIDEGLYSRQLYVLGHEAMKRMQNANILVSGLRGLGVEIAKNIILGGVKSVTVHDQGVAEWSDLSSQFYLHEEDLGKNRAEVSQPRLAELNSYVPVTAYTGPLSEDFLGTFQVVVLTNCPLEEQLRISDFCHSQDIKLVVADTKGLFGQLFCDFGDEMVVTDTNGEQPLSAMVSMVTKGCPGEVTCLDEARHGFESGDFVSFTEVEGMTQLNSSEPMEIKVLGPYTFSIGDTSSFSDYVRGGIVTQVKMPKKISFKSLRRSLQEPEFILTDFGKFDRPAQLHFGFQALHEFHKKHGHFPKPRNQADAAEVLTLTKELNEQAAPPLKQEQLNEDVVKELAFQATGDLAPINAFVGGLAAQEVMKACSGKFMPIAQWLYFDALECLPEENKDALLTEENCTPKHCRYDGQIAVFGSDLQAKLGQQKYFLVGAGAIGCELLKNFAMIGLGCGQGGEIVVTDMDTIEKSNLNRQFLFRPWDVTKMKSDTAAASVKEMNPHINIVSHQDRVGPDTERVYDDDFFENLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNIQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYITDAKFMERTQKLPGTQPLEVLEAVYKSLVTDRPKTWADCIAWACNHWHTQYCNNIRQLLHNFPPNQKTNSGTLFWSGPKRCPHALTFDTNNPLHMDYVVAGANLFAQTYGITGTRDRAAIAELLRQVQVPEFTPKSGVRIHISDQELQNANASVDDSRLEELKSSLPSPQQLQDFRMFPVDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVIQGHQRLESFKNGFLNLALPFFAFSEPVACPKNKYYSTEWTLWDRFEVQGIQPDGQEMTLREFLAYFKKEHKLEITMLSQGVSMLYSFFMQPAKLKERHDQPMTEIVTRVSKKKIGRHVKALVFELCCNDDSGDDTEVPYVRYTIR

Target ORF information:

RefSeq Version	XM_015406175.1
Organism	Gekko japonicus
Definition	Gekko japonicus ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015406175.1

ORF Insert Sequence:

ATGTCCAGCT CGCCGTTGTC CAAGAAGCGT CGCTTGTCTG AATCTGAGAC GAAGTCGGGT 
TCAAACTGCT CCTCTGGCAA ATTGGTACAG ACTGACCTAC GCGAAGCGCC TGCCAACGGT 
ATGGCCAAGA ATGGAAGCGA AGCAGACATT GATGAGGGTC TGTACTCGAG ACAGCTGTAT 
GTGCTGGGCC ATGAGGCCAT GAAACGGATG CAGAATGCCA ACATTCTGGT CTCTGGGCTG 
CGAGGACTGG GCGTGGAGAT CGCCAAGAAC ATCATCTTGG GCGGAGTCAA ATCCGTCACA 
GTCCATGACC AGGGGGTGGC TGAGTGGTCG GACCTTTCCT CCCAGTTCTA TCTGCATGAG 
GAGGACCTGG GGAAAAATCG GGCGGAAGTA TCCCAGCCAC GACTGGCAGA GCTCAACTCA 
TACGTACCCG TTACGGCTTA CACTGGACCC CTGAGCGAGG ACTTCCTGGG CACCTTCCAG 
GTGGTCGTGC TCACCAATTG CCCCCTGGAA GAGCAGCTGC GCATTAGTGA CTTCTGTCAC 
AGCCAAGACA TCAAACTGGT AGTGGCGGAT ACCAAGGGAC TGTTTGGCCA GTTGTTTTGT 
GACTTTGGTG ATGAGATGGT TGTGACGGAC ACCAACGGGG AACAGCCGCT CAGTGCTATG 
GTCTCCATGG TCACCAAGGG ATGCCCCGGA GAGGTAACCT GCCTAGATGA AGCCCGGCAT 
GGCTTTGAGA GCGGGGACTT TGTTTCCTTC ACTGAAGTGG AGGGCATGAC ACAACTCAAC 
AGCAGTGAAC CCATGGAAAT CAAAGTGCTC GGCCCCTACA CATTCAGCAT TGGTGATACC 
TCCAGCTTTT CGGACTATGT ACGCGGTGGC ATCGTCACCC AGGTTAAGAT GCCAAAGAAG 
ATTAGCTTTA AATCTCTACG TCGTTCCCTG CAAGAGCCAG AATTCATACT CACCGACTTT 
GGCAAGTTTG ATAGGCCAGC CCAGCTGCAC TTTGGGTTCC AGGCTTTGCA TGAATTCCAC 
AAGAAGCATG GCCATTTCCC CAAGCCCCGT AACCAGGCAG ACGCTGCTGA GGTGCTGACG 
TTGACCAAGG AACTGAATGA ACAGGCAGCA CCCCCCCTTA AGCAAGAGCA GCTGAATGAG 
GACGTCGTCA AAGAACTGGC CTTCCAGGCT ACCGGGGATC TGGCCCCCAT CAATGCCTTT 
GTCGGAGGAC TGGCTGCACA GGAAGTCATG AAGGCGTGTT CGGGGAAATT CATGCCCATC 
GCTCAGTGGC TATACTTTGA TGCCCTGGAG TGCCTGCCAG AGGAAAACAA AGACGCCCTA 
CTGACGGAGG AGAACTGCAC CCCTAAACAC TGCCGATACG ATGGACAGAT TGCCGTCTTC 
GGCAGCGACC TGCAGGCTAA GCTGGGTCAG CAGAAGTACT TCCTGGTTGG TGCCGGTGCC 
ATTGGCTGCG AGCTGCTGAA GAACTTTGCC ATGATTGGTC TGGGCTGCGG TCAAGGCGGA 
GAAATAGTTG TGACGGACAT GGACACCATT GAGAAATCCA ACCTCAACAG GCAGTTCCTA 
TTCCGGCCTT GGGATGTGAC GAAAATGAAA TCAGATACAG CGGCTGCCTC TGTGAAAGAA 
ATGAACCCAC ACATCAACAT TGTGAGCCAC CAAGACCGCG TGGGCCCCGA CACTGAACGT 
GTTTATGATG ATGACTTCTT TGAGAACCTT GATGGAGTGG CAAATGCGTT GGACAATGTA 
GATGCCCGGA TGTACATGGA CCGTCGCTGT GTCTACTACC GCAAGCCCCT GCTGGAATCG 
GGGACGCTGG GGACCAAAGG GAACATCCAG GTGGTGATCC CCTTCCTGAC AGAGTCCTAC 
AGCTCCAGCC AGGACCCGCC CGAAAAGTCC ATCCCCATCT GCACTCTGAA GAACTTTCCC 
AATGCCATCG AGCACACCCT GCAGTGGGCC CGTGATGAAT TTGAAGGTCT TTTCAAGCAG 
CCTGCAGAAA ATGTCAACCA GTACATCACA GACGCCAAGT TCATGGAACG GACCCAGAAA 
CTGCCTGGCA CCCAACCTCT GGAGGTGTTA GAAGCTGTCT ACAAGAGCTT GGTGACTGAC 
CGGCCTAAGA CGTGGGCGGA CTGCATAGCC TGGGCATGCA ATCATTGGCA TACCCAGTAC 
TGCAACAACA TTCGTCAGCT GCTGCACAAT TTCCCTCCAA ACCAGAAAAC CAACTCGGGA 
ACCCTCTTCT GGTCGGGTCC CAAGCGATGC CCTCACGCCC TCACCTTCGA TACCAACAAT 
CCCTTGCACA TGGATTATGT GGTGGCAGGT GCGAACTTGT TTGCACAGAC CTACGGCATC 
ACAGGCACCA GGGACAGGGC GGCCATCGCG GAGCTGCTGC GCCAGGTCCA GGTCCCAGAA 
TTCACTCCCA AGTCGGGGGT GCGCATCCAC ATCTCCGACC AAGAGCTGCA GAACGCCAAC 
GCTTCAGTCG ACGACAGCCG GCTGGAGGAA CTGAAATCTT CACTCCCAAG TCCCCAGCAA 
CTGCAAGACT TCAGGATGTT TCCGGTCGAT TTTGAAAAGG ATGATGACAC GAACTTCCAC 
ATGGATTTCA TTGTGGCTGC GTCCAACCTG CGGGCAGAGA ACTACGATAT TCCTCCTGCT 
GACCGGCATA AGAGCAAGTT GATCGCCGGG AAGATTATAC CCGCCATCGC CACGACGACA 
GCAGCTGTAG TAGGCTTGGT GTGTCTGGAA CTCTACAAGG TGATACAGGG CCACCAGCGA 
CTTGAGTCCT TCAAGAATGG CTTCCTCAAC TTGGCGTTGC CTTTCTTTGC TTTCTCTGAG 
CCCGTTGCCT GCCCCAAGAA CAAGTATTAT AGCACTGAAT GGACTCTGTG GGACCGTTTT 
GAAGTGCAGG GCATCCAGCC CGACGGGCAG GAGATGACGC TGCGCGAATT CCTGGCCTAC 
TTCAAGAAAG AGCACAAGTT GGAGATTACG ATGTTGTCCC AGGGAGTCTC CATGCTGTAC 
TCGTTCTTCA TGCAGCCGGC GAAGCTGAAG GAGCGCCACG ACCAGCCGAT GACGGAGATT 
GTCACCAGAG TCTCCAAGAA GAAGATCGGC CGCCACGTGA AGGCCTTGGT CTTTGAGCTG 
TGCTGTAACG ACGACAGTGG GGACGACACG GAGGTGCCCT ACGTGCGCTA CACCATCCGC 
TAG