LRRN1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRN1
Entrez Gene ID	771599
Full Name	leucine rich repeat neuronal 1
Synonyms	LRCH4
General protein information	Preferred Names leucine rich repeat neuronal 1 Names leucine-rich repeat neuronal protein 1 leucine-rich repeats and calponin homology (CH) domain containing 4
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001097539.1	NP_001091008.1	leucine-rich repeat neuronal protein 1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LRRN1	NP_001074207.1
Homo sapiens (human)	LRRN1	NP_065924.3
Pan troglodytes (chimpanzee)	LRRN1	XP_526119.2
Rattus norvegicus (Norway rat)	Lrrn1	NP_001032440.1
Gallus gallus (chicken)	LRRN1	NP_001091008.1
Xenopus tropicalis (tropical clawed frog)	LOC100492182	XP_002938357.1
Danio rerio (zebrafish)	lrrn1	NP_001124166.1
	LRRN1	XP_001099632.1
Canis lupus familiaris (dog)	LRRN1	XP_003432798.1
Mus musculus (house mouse)	Lrrn1	NP_032542.1

Gene Ontology
Bibliography

Related articles in PubMed

Lrrn1 is required for formation of the midbrain-hindbrain boundary and organiser through regulation of affinity differences between midbrain and hindbrain cells in chick.
Tossell K, Andreae LC, Cudmore C, Lang E, Muthukrishnan U, Lumsden A, Gilthorpe JD, Irving C
Developmental biology352(2)341-52(2011 Apr)

Analysis of Lrrn1 expression and its relationship to neuromeric boundaries during chick neural development.
Andreae LC, Peukert D, Lumsden A, Gilthorpe JD
Neural development222(2007 Oct)

Expression of Lrrn1 marks the prospective site of the zona limitans thalami in the early embryonic chicken diencephalon.
Garc?a-Calero E, Garda AL, Mar?n F, Puelles L
Gene expression patterns : GEP6(8)879-85(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lrrn1 is expressed in midbrain cells but not in anterior hindbrain cells. Lrrn1 is down-regulated in the anterior hindbrain by the organiser signalling molecule FGF8, thereby creating a differential affinity between these two compartments.
Title: Lrrn1 is required for formation of the midbrain-hindbrain boundary and organiser through regulation of affinity differences between midbrain and hindbrain cells in chick.

Lrrn1 may regulate the subcellular localisation of specific components of signalling or cell-cell recognition pathways in neuroepithelial cells.
Title: Analysis of Lrrn1 expression and its relationship to neuromeric boundaries during chick neural development.

Lrrn1's expression preconfigures the zona limitans thalami site before overt formation of this boundary structure
Title: Expression of Lrrn1 marks the prospective site of the zona limitans thalami in the early embryonic chicken diencephalon.

The following LRRN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa24621	NM_001097539.1 Latest version!	Gallus gallus leucine rich repeat neuronal 1 (LRRN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRN1 ( NM_001097539.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001091008.1 Gallus gallus leucine rich repeat neuronal 1 (LRRN1), mRNA.

Price & Availability↑

CloneID	OGa24621
Clone ID Related Accession (Same CDS sequence)	NM_001097539.1
Accession Version	NM_001097539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	leucine-rich repeat neuronal protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF512462.1. On May 24, 2007 this sequence version replaced XM_001234385.1. ##Evidence-Data-START## Transcript exon combination :: EF512462.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTAAGA TTAGATTAGT TTTAACTGTT TGCCAGGTGG TGCTAGAATT GCTAACGAAT 
TCATTAACTG AGTCTTCCGT ACAGAGTAAT GAATGTCCCC AGCTTTGTGT ATGCGAAATC 
AGGCCATGGT TTACACCACA GTCAACATAC AGGGAAGCCA CAACAGTTGA CTGCAATGAC 
CTTCGTTTAA CAAAAATCCC CAGCAATCTT TCCAGTGACA CTCAAGTCCT TCTGTTACAA 
AGCAACAATA TCGCAAAGAC CACAGATGAA CTCCAACTGC TATTTAATTT AACAGAGTTA 
GATTTTTCAC AAAATAACTT CACAAGTATC AGAGACGTGG GGCTCTCAAA TCTTACTCAG 
CTAACTACTT TGCATCTGGA GGAAAATCAG ATCATGGAGA TGACTGACTA CTGCTTGCAA 
GACCTTTGCA ATCTTCAGGA GTTATATATA AATCATAATC AGATCAGCAG CATTTCTGCA 
AATGCATTCT CTGGCCTGAA GAATCTTTTA AGATTACATC TCAACTCCAA CAAATTAAAG 
GTTATTGACA GCCGTTGGTT TGATTCTACT CCTAACTTAG AGATTCTTAT GATTGGAGAA 
AATCCAGTGA TTGGAATACT AGATATGAAC TTCAAACCAC TCTCCAATTT AAGGAGTCTT 
GTTTTGGCGG GTATGTATCT TACAGACATC CCCGGCAATG CCTTGGTAGG CTTGGACAGT 
CTTGAAAGTC TTTCCTTCTA TGACAACAAA TTGGTAAAAG TTCCTCAGCT CGCACTTGAG 
AAAGTTCCAA ATCTAAAATT CCTGGATCTC AACAAAAATC CAATTCATAA AATTCAAGAA 
GGTGACTTTA GAAACATGCT CAGATTGAAA GAGCTTGGAA TCAATAATAT GGGAGAGCTT 
GTTTCTGTGG ATAGGTATGC GCTGGACAAC CTGCCTGAAC TTACAAAGCT GGAAGCCACC 
AACAACCCAA AGCTGTCCTA CATCCATCGT TTGGCATTTC GCAACGTCCC TGCCCTGGAG 
AGTTTGATGC TGAACAACAA CGCCTTGAAC GCTGTCTACC AAAAAACAGT GGAGTCCCTC 
CCAAACCTGC GTGAGATTAG CATCCACAGC AACCCCCTCC GGTGCGACTG TGTCATTCAC 
TGGATCAACT CAAACAAAAC CAACATCCGC TTCATGGAAC CTCTCTCCAT GTTCTGTGCC 
ATGCCTCCAG AGTACAGAGG ACAACAGGTA AAAGAAGTGC TAATACAGGA TTCAAACGAG 
CAATGTCTTC CAATGATCTC TCACGAGACC TTTCCAAATC ACTTAAACTT GGACATTGGC 
ATGACAGTAT TTTTAGACTG CAGGGCCATG GCAGAACCCG AGCCAGAAAT TTACTGGGTC 
ACTCCCCTCG GAAATAAAGT AACTGTTGAA AGCCTCTCTG ACAAATACAA GCTAAGCAGC 
GAAGGCACCT TGGAAATCTC CAATATTCAG ATTGAAGACT CTGGGAGGTA CACCTGTGTT 
GCTCAGAACA TAGAAGGAGC TGACACGAGG GTCGCCACAA TCCGGGTGAA CGGAACACTT 
CTGGATGGCA CCCAGGTTCT GAAAATCTTT GTGAAGCAAG CTGAATCGCA TTCCATTTTA 
GTTTCTTGGA AAGTTAACTC CAATGTCATG ACTTCCAATT TAAAGTGGTC ATCAGCTACT 
ATGAAGATTG ACAACCCTCA CATTACATAC ACCGCTCGGG TCCCAGTTGA TGTACATGAA 
TATAACCTCA CGCATTTACA ACCTTCTACA GATTATGAAG TGTGTCTCAC TGTGTCCAAC 
ATCCACCAAC AAACACAAAA GTCCTGTGTT AATGTTACAA CAAAAAATGC AGCTTTTGCG 
CTGGATATTT CAGACCAAGA AACCAGCACT GCTCTGGCAG CGGTAATGGG ATCGATGTTT 
GCTGTAATTA GCCTTGCCTC CATTTCTGTT TACATTGCAA AAAGATTTAA GAGAAAAAAC 
TACCACCATT CACTGAAAAA ATATATGCAA AAGACCTCCT CAATCCCCCT GAATGAGCTC 
TATCCTCCAC TTATTAATCT CTGGGAAGGC GACAGCGATA AGGACAAGGA TGGTTCTGCA 
GAGACCAAGC CAACCCAAGT TGACACATCC AGAAGCTATT ACATGTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091008.1
CDS	365..2515
Translation	MAKIRLVLTVCQVVLELLTNSLTESSVQSNECPQLCVCEIRPWFTPQSTYREATTVDCNDLRLTKIPSNLSSDTQVLLLQSNNIAKTTDELQLLFNLTELDFSQNNFTSIRDVGLSNLTQLTTLHLEENQIMEMTDYCLQDLCNLQELYINHNQISSISANAFSGLKNLLRLHLNSNKLKVIDSRWFDSTPNLEILMIGENPVIGILDMNFKPLSNLRSLVLAGMYLTDIPGNALVGLDSLESLSFYDNKLVKVPQLALEKVPNLKFLDLNKNPIHKIQEGDFRNMLRLKELGINNMGELVSVDRYALDNLPELTKLEATNNPKLSYIHRLAFRNVPALESLMLNNNALNAVYQKTVESLPNLREISIHSNPLRCDCVIHWINSNKTNIRFMEPLSMFCAMPPEYRGQQVKEVLIQDSNEQCLPMISHETFPNHLNLDIGMTVFLDCRAMAEPEPEIYWVTPLGNKVTVESLSDKYKLSSEGTLEISNIQIEDSGRYTCVAQNIEGADTRVATIRVNGTLLDGTQVLKIFVKQAESHSILVSWKVNSNVMTSNLKWSSATMKIDNPHITYTARVPVDVHEYNLTHLQPSTDYEVCLTVSNIHQQTQKSCVNVTTKNAAFALDISDQETSTALAAVMGSMFAVISLASISVYIAKRFKRKNYHHSLKKYMQKTSSIPLNELYPPLINLWEGDSDKDKDGSAETKPTQVDTSRSYYMW

Target ORF information:

RefSeq Version	NM_001097539.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus leucine rich repeat neuronal 1 (LRRN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097539.1

ORF Insert Sequence:

ATGGCTAAGA TTAGATTAGT TTTAACTGTT TGCCAGGTGG TGCTAGAATT GCTAACGAAT 
TCATTAACTG AGTCTTCCGT ACAGAGTAAT GAATGTCCCC AGCTTTGTGT ATGCGAAATC 
AGGCCATGGT TTACACCACA GTCAACATAC AGGGAAGCCA CAACAGTTGA CTGCAATGAC 
CTTCGTTTAA CAAAAATCCC CAGCAATCTT TCCAGTGACA CTCAAGTCCT TCTGTTACAA 
AGCAACAATA TCGCAAAGAC CACAGATGAA CTCCAACTGC TATTTAATTT AACAGAGTTA 
GATTTTTCAC AAAATAACTT CACAAGTATC AGAGACGTGG GGCTCTCAAA TCTTACTCAG 
CTAACTACTT TGCATCTGGA GGAAAATCAG ATCATGGAGA TGACTGACTA CTGCTTGCAA 
GACCTTTGCA ATCTTCAGGA GTTATATATA AATCATAATC AGATCAGCAG CATTTCTGCA 
AATGCATTCT CTGGCCTGAA GAATCTTTTA AGATTACATC TCAACTCCAA CAAATTAAAG 
GTTATTGACA GCCGTTGGTT TGATTCTACT CCTAACTTAG AGATTCTTAT GATTGGAGAA 
AATCCAGTGA TTGGAATACT AGATATGAAC TTCAAACCAC TCTCCAATTT AAGGAGTCTT 
GTTTTGGCGG GTATGTATCT TACAGACATC CCCGGCAATG CCTTGGTAGG CTTGGACAGT 
CTTGAAAGTC TTTCCTTCTA TGACAACAAA TTGGTAAAAG TTCCTCAGCT CGCACTTGAG 
AAAGTTCCAA ATCTAAAATT CCTGGATCTC AACAAAAATC CAATTCATAA AATTCAAGAA 
GGTGACTTTA GAAACATGCT CAGATTGAAA GAGCTTGGAA TCAATAATAT GGGAGAGCTT 
GTTTCTGTGG ATAGGTATGC GCTGGACAAC CTGCCTGAAC TTACAAAGCT GGAAGCCACC 
AACAACCCAA AGCTGTCCTA CATCCATCGT TTGGCATTTC GCAACGTCCC TGCCCTGGAG 
AGTTTGATGC TGAACAACAA CGCCTTGAAC GCTGTCTACC AAAAAACAGT GGAGTCCCTC 
CCAAACCTGC GTGAGATTAG CATCCACAGC AACCCCCTCC GGTGCGACTG TGTCATTCAC 
TGGATCAACT CAAACAAAAC CAACATCCGC TTCATGGAAC CTCTCTCCAT GTTCTGTGCC 
ATGCCTCCAG AGTACAGAGG ACAACAGGTA AAAGAAGTGC TAATACAGGA TTCAAACGAG 
CAATGTCTTC CAATGATCTC TCACGAGACC TTTCCAAATC ACTTAAACTT GGACATTGGC 
ATGACAGTAT TTTTAGACTG CAGGGCCATG GCAGAACCCG AGCCAGAAAT TTACTGGGTC 
ACTCCCCTCG GAAATAAAGT AACTGTTGAA AGCCTCTCTG ACAAATACAA GCTAAGCAGC 
GAAGGCACCT TGGAAATCTC CAATATTCAG ATTGAAGACT CTGGGAGGTA CACCTGTGTT 
GCTCAGAACA TAGAAGGAGC TGACACGAGG GTCGCCACAA TCCGGGTGAA CGGAACACTT 
CTGGATGGCA CCCAGGTTCT GAAAATCTTT GTGAAGCAAG CTGAATCGCA TTCCATTTTA 
GTTTCTTGGA AAGTTAACTC CAATGTCATG ACTTCCAATT TAAAGTGGTC ATCAGCTACT 
ATGAAGATTG ACAACCCTCA CATTACATAC ACCGCTCGGG TCCCAGTTGA TGTACATGAA 
TATAACCTCA CGCATTTACA ACCTTCTACA GATTATGAAG TGTGTCTCAC TGTGTCCAAC 
ATCCACCAAC AAACACAAAA GTCCTGTGTT AATGTTACAA CAAAAAATGC AGCTTTTGCG 
CTGGATATTT CAGACCAAGA AACCAGCACT GCTCTGGCAG CGGTAATGGG ATCGATGTTT 
GCTGTAATTA GCCTTGCCTC CATTTCTGTT TACATTGCAA AAAGATTTAA GAGAAAAAAC 
TACCACCATT CACTGAAAAA ATATATGCAA AAGACCTCCT CAATCCCCCT GAATGAGCTC 
TATCCTCCAC TTATTAATCT CTGGGAAGGC GACAGCGATA AGGACAAGGA TGGTTCTGCA 
GAGACCAAGC CAACCCAAGT TGACACATCC AGAAGCTATT ACATGTGGTA A