HCN2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCN2
Entrez Gene ID	770384
Full Name	hyperpolarization activated cyclic nucleotide-gated potassium channel 2
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide-gated potassium channel 2 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2-like
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003642867.3	XP_003642915.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
XM_003642867.4	XP_003642915.2	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN2	NP_001185.3
Gallus gallus (chicken)	HCN2	XP_003642915.1
Xenopus tropicalis (tropical clawed frog)	hcn2	XP_002939815.2
Canis lupus familiaris (dog)	HCN2	XP_005642548.1
Rattus norvegicus (Norway rat)	Hcn2	NP_446136.1
Pan troglodytes (chimpanzee)	LOC467640	XP_003952669.1
Bos taurus (cattle)	HCN2	XP_002689207.2
Mus musculus (house mouse)	Hcn2	NP_032252.1
Danio rerio (zebrafish)	hcn2b	XP_685414.3

Gene Ontology

The following HCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa148506	XM_003642867.4 Latest version!	Gallus gallus hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa20478	XM_003642867.3 Latest version!	Gallus gallus hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCN2 ( XM_003642867.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003642915.2 Gallus gallus hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2), mRNA.

Price & Availability↑

CloneID	OGa148506
Clone ID Related Accession (Same CDS sequence)	XM_003642867.4
Accession Version	XM_003642867.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006115.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003642867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG TGGGAGCACC GCCGGGGTTG CGCACCCGGG GGCTGCGCCA GCGGGGGGAG 
CTGCGGAGCC GCTGCGGTCC CGTCGCCCGC CCCCGGCCCG GCCCTCCGGC CCGCCCCCGG 
CCCGCCCCAC GGCGCGGCGG CGGGTACCGC TCGGGTACCG CTCGGGTACC ACTCGGCCGC 
CGGTGGCCTC GGCGGCAGCG CACGATGCGA TCGGGCGGGG AGGCGGCGGC AGAGGAGGAG 
GCGGCCGAAG GGGCCAAACG CGGCGGAGGG GCGGCCGCGG GGAGAGCGCG GGGCCGCGGG 
GGGAAAGGAT CCCCGAACGG GGAGTGCCGC CGCGGGGAAA CGCCGCGCAG CCCCAGCGCG 
GAGCAGCCCC GGGAGCCGAA GGTCTCCTTC TCCTGCGGCG GTGGCGGCGG CGGCGGCGGC 
GGCGGCAGCG CATCCCCAGG CGGGGCCAAG GCGGCCGAGG AAGGCGACGA TGCGGGCGAG 
GAGGTTCGCG GGAGCCAGGC CAGCTTCATG CAGAGGCAGT TCGGGGCCAT GCTCCAGCCC 
GGCGTCAACA AGTTCTCGCT GCGGATGTTC GGTTCCCAGA AGGCGGTGGA ACGGGAGCAG 
GAACGCGTTA AGTCAGCGGG GGCCTGGATC ATCCATCCCT ACAGCGATTT CAGGTTCTAC 
TGGGACTTCA CGATGCTGCT CTTCATGGTG GGCAACCTGA TCATCATTCC CGTGGGCATC 
ACCTTCTTCA AGGAGGAAAC CACGGCGCCC TGGATCGTCT TCAACGTGGT CTCCGACACC 
TTCTTCCTGA TGGACCTGGT GCTGAACTTC CGCACGGGGA TCGTCATCGA GGACAACACC 
GAGATCATCC TGGACCCCGA AAGGATCAAG AAGAAGTACC TGAAGACGTG GTTTGTGGTG 
GACTTCGTCT CCTCCATCCC CGTGGACTAC GTCTTCCTCA TTGTGGAGAA GGGCATCGAC 
TCGGAGGTCT ATAAGACAGC CCGTGCTCTC CGCATCGTCC GCTTCACCAA GATCCTCAGC 
CTGCTGCGCC TGCTGCGCCT CTCCCGCCTC ATCCGCTACA TCCACCAGTG GGAGGAGATC 
TTCCACATGA CCTACGACCT GGCCAGTGCC GTGATGAGGA TCATTAACCT CATTGGCATG 
ATGCTGCTGC TGTGCCACTG GGACGGCTGT CTCCAGTTCC TCGTGCCCAT GCTGCAGGAT 
TTCCCCCAGA ACTGCTGGGT CTCCATCAAT GGGATGGTGA ACGACTCCTG GAGCGAGCTG 
TACTCCTTCG CCCTCTTCAA GTCCATGAGC CACATGCTGT GCATCGGCTA CGGCAAGCAG 
GCGCCCGAGA GCATGACGGA CATCTGGCTG ACGATGCTGA GCATGATCGT GGGAGCCACC 
TGCTATGCCA TGTTCATCGG CCACGCCACC GCCCTCATCC AGTCTCTGGA CTCCTCCCGG 
CGCCAGTATC AGGAGAAGTA CAAGCAGGTG GAGCAGTACA TGTCCTTCCA CAAGCTGCCC 
GCTGACTTCA GACAGAAGAT CCACGACTAC TACGAGCACC GCTACCAGGG CAAGATGTTC 
GACGAGGACA GCATCCTTGG GGAGCTCAAC GAGCCCTTAC GTGAGGAAAT CGTGAACTTC 
AACTGCCGCA AACTGGTGGC CTCCATGCCT CTGTTTGCCA ACGCGGACCC CAACTTCGTG 
ACGGCGATGC TCACCAAGCT GAAGTTTGAG GTGTTCCAGC CAGGCGACTA CATCATCCGC 
GAGGGGACCA TCGGCAAGAA GATGTACTTC ATCCAGCACG GCGTGGTCAG CATCCTCACC 
AAGGGCAACA AGGAGATGAA GCTCTCTGAT GGCTCCTACT TCGGGGAAAT CTGCCTGCTG 
ACCCGTGGTC GGCGCACAGC CAGCGTCCGG GCGGACACCT ACTGCCGCCT CTACTCGCTG 
TCAGTGGACA ACTTCAACGA GGTGCTGGAG GAGTACCCCA TGATGAGGAG AGCCTTTGAG 
ACCGTGGCCA TTGACCGCCT TGATCGCATC GGGAAAAAGA ACTCGATCCT TCTTCACAAA 
GTGCAGCACG ACCTCAACTC GGGCGTCTTC AACAACCAGG AGAACGAAAT CATCCAGGAG 
ATTGTCAAGT ATGACCGAGA GATGGTCCAG CAGGCGGAGC TGCAGCAGCA CACGGCCATG 
TACAGCCCGG TGCAACCCCA GGTCACCTCT GCCATTGCCA CCTTGCAGCA AGCCGTCGCC 
ATGAGCTTCT GCCCACAGAT GGCCAGCCCG CTGGTGGGCT CCATGGCTCT GGGCTCGCCC 
CGCATGATGC GTCGCTTGCA ATACGCCCAG GCCGTGCCCA GCCCCTTCGC CGTCTCCCCA 
GTGCTGCTGC AGCAGAGCCC CCCGCAGCAG CCGCAGCCCC CCGTGCCCCA CGCCAACCCA 
TCGCCCTCGC AGGACCAGGC ACAGCCCGCC TCCGCCAGCG CCTTCGCTGC TGCCAGCCCG 
CCGTCCCAAA GCCCCTTGGC CAGCCGGACG TTTGCCTACG GAGGGGCCAA GGGGCAGCTG 
GGCTCCCAGC TCTCACTGAG CCAGCAGCAG ACGCCCGGCT CGCCCCCACG GTTGGCCGTT 
CACAAAAGCA CACAGGCGCT GCCCACCAGC AGCTTCAGCC AGGATTCACG GCCTCTATCG 
GCTTCGCAGC CCTCCCTGCC CCACGGGCTG GCGGCTGGCA GCACCCAGAG CCCCCCGGCC 
TCAGTGCACG AATCCTCTGC TTCCATCGGT GGAGGCCCCG CCGCCACCGC CGTCTCGCCG 
GGTTCGGGTC CCCCAGCCGG GCTGCGGGGC GCAGTGTCCT CGAGGGGGCC CCTGTCTCAC 
CCTGCGCCCA CCCTGCAGCA GGACTCGGCG GCAGCCCGCA AGGATTCGGT GGGCAGCATG 
CCCGACACGG ACCCAGCCAA ATCCAGGCTG TCTTCCAACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003642915.2
CDS	16..2940
Translation	MGAVGAPPGLRTRGLRQRGELRSRCGPVARPRPGPPARPRPAPRRGGGYRSGTARVPLGRRWPRRQRTMRSGGEAAAEEEAAEGAKRGGGAAAGRARGRGGKGSPNGECRRGETPRSPSAEQPREPKVSFSCGGGGGGGGGGSASPGGAKAAEEGDDAGEEVRGSQASFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKEETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPERIKKKYLKTWFVVDFVSSIPVDYVFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRIINLIGMMLLLCHWDGCLQFLVPMLQDFPQNCWVSINGMVNDSWSELYSFALFKSMSHMLCIGYGKQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNEPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSILTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENEIIQEIVKYDREMVQQAELQQHTAMYSPVQPQVTSAIATLQQAVAMSFCPQMASPLVGSMALGSPRMMRRLQYAQAVPSPFAVSPVLLQQSPPQQPQPPVPHANPSPSQDQAQPASASAFAAASPPSQSPLASRTFAYGGAKGQLGSQLSLSQQQTPGSPPRLAVHKSTQALPTSSFSQDSRPLSASQPSLPHGLAAGSTQSPPASVHESSASIGGGPAATAVSPGSGPPAGLRGAVSSRGPLSHPAPTLQQDSAAARKDSVGSMPDTDPAKSRLSSNL

Target ORF information:

RefSeq Version	XM_003642867.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003642867.4

ORF Insert Sequence:

ATGGGAGCGG TGGGAGCACC GCCGGGGTTG CGCACCCGGG GGCTGCGCCA GCGGGGGGAG 
CTGCGGAGCC GCTGCGGTCC CGTCGCCCGC CCCCGGCCCG GCCCTCCGGC CCGCCCCCGG 
CCCGCCCCAC GGCGCGGCGG CGGGTACCGC TCGGGTACCG CTCGGGTACC ACTCGGCCGC 
CGGTGGCCTC GGCGGCAGCG CACGATGCGA TCGGGCGGGG AGGCGGCGGC AGAGGAGGAG 
GCGGCCGAAG GGGCCAAACG CGGCGGAGGG GCGGCCGCGG GGAGAGCGCG GGGCCGCGGG 
GGGAAAGGAT CCCCGAACGG GGAGTGCCGC CGCGGGGAAA CGCCGCGCAG CCCCAGCGCG 
GAGCAGCCCC GGGAGCCGAA GGTCTCCTTC TCCTGCGGCG GTGGCGGCGG CGGCGGCGGC 
GGCGGCAGCG CATCCCCAGG CGGGGCCAAG GCGGCCGAGG AAGGCGACGA TGCGGGCGAG 
GAGGTTCGCG GGAGCCAGGC CAGCTTCATG CAGAGGCAGT TCGGGGCCAT GCTCCAGCCC 
GGCGTCAACA AGTTCTCGCT GCGGATGTTC GGTTCCCAGA AGGCGGTGGA ACGGGAGCAG 
GAACGCGTTA AGTCAGCGGG GGCCTGGATC ATCCATCCCT ACAGCGATTT CAGGTTCTAC 
TGGGACTTCA CGATGCTGCT CTTCATGGTG GGCAACCTGA TCATCATTCC CGTGGGCATC 
ACCTTCTTCA AGGAGGAAAC CACGGCGCCC TGGATCGTCT TCAACGTGGT CTCCGACACC 
TTCTTCCTGA TGGACCTGGT GCTGAACTTC CGCACGGGGA TCGTCATCGA GGACAACACC 
GAGATCATCC TGGACCCCGA AAGGATCAAG AAGAAGTACC TGAAGACGTG GTTTGTGGTG 
GACTTCGTCT CCTCCATCCC CGTGGACTAC GTCTTCCTCA TTGTGGAGAA GGGCATCGAC 
TCGGAGGTCT ATAAGACAGC CCGTGCTCTC CGCATCGTCC GCTTCACCAA GATCCTCAGC 
CTGCTGCGCC TGCTGCGCCT CTCCCGCCTC ATCCGCTACA TCCACCAGTG GGAGGAGATC 
TTCCACATGA CCTACGACCT GGCCAGTGCC GTGATGAGGA TCATTAACCT CATTGGCATG 
ATGCTGCTGC TGTGCCACTG GGACGGCTGT CTCCAGTTCC TCGTGCCCAT GCTGCAGGAT 
TTCCCCCAGA ACTGCTGGGT CTCCATCAAT GGGATGGTGA ACGACTCCTG GAGCGAGCTG 
TACTCCTTCG CCCTCTTCAA GTCCATGAGC CACATGCTGT GCATCGGCTA CGGCAAGCAG 
GCGCCCGAGA GCATGACGGA CATCTGGCTG ACGATGCTGA GCATGATCGT GGGAGCCACC 
TGCTATGCCA TGTTCATCGG CCACGCCACC GCCCTCATCC AGTCTCTGGA CTCCTCCCGG 
CGCCAGTATC AGGAGAAGTA CAAGCAGGTG GAGCAGTACA TGTCCTTCCA CAAGCTGCCC 
GCTGACTTCA GACAGAAGAT CCACGACTAC TACGAGCACC GCTACCAGGG CAAGATGTTC 
GACGAGGACA GCATCCTTGG GGAGCTCAAC GAGCCCTTAC GTGAGGAAAT CGTGAACTTC 
AACTGCCGCA AACTGGTGGC CTCCATGCCT CTGTTTGCCA ACGCGGACCC CAACTTCGTG 
ACGGCGATGC TCACCAAGCT GAAGTTTGAG GTGTTCCAGC CAGGCGACTA CATCATCCGC 
GAGGGGACCA TCGGCAAGAA GATGTACTTC ATCCAGCACG GCGTGGTCAG CATCCTCACC 
AAGGGCAACA AGGAGATGAA GCTCTCTGAT GGCTCCTACT TCGGGGAAAT CTGCCTGCTG 
ACCCGTGGTC GGCGCACAGC CAGCGTCCGG GCGGACACCT ACTGCCGCCT CTACTCGCTG 
TCAGTGGACA ACTTCAACGA GGTGCTGGAG GAGTACCCCA TGATGAGGAG AGCCTTTGAG 
ACCGTGGCCA TTGACCGCCT TGATCGCATC GGGAAAAAGA ACTCGATCCT TCTTCACAAA 
GTGCAGCACG ACCTCAACTC GGGCGTCTTC AACAACCAGG AGAACGAAAT CATCCAGGAG 
ATTGTCAAGT ATGACCGAGA GATGGTCCAG CAGGCGGAGC TGCAGCAGCA CACGGCCATG 
TACAGCCCGG TGCAACCCCA GGTCACCTCT GCCATTGCCA CCTTGCAGCA AGCCGTCGCC 
ATGAGCTTCT GCCCACAGAT GGCCAGCCCG CTGGTGGGCT CCATGGCTCT GGGCTCGCCC 
CGCATGATGC GTCGCTTGCA ATACGCCCAG GCCGTGCCCA GCCCCTTCGC CGTCTCCCCA 
GTGCTGCTGC AGCAGAGCCC CCCGCAGCAG CCGCAGCCCC CCGTGCCCCA CGCCAACCCA 
TCGCCCTCGC AGGACCAGGC ACAGCCCGCC TCCGCCAGCG CCTTCGCTGC TGCCAGCCCG 
CCGTCCCAAA GCCCCTTGGC CAGCCGGACG TTTGCCTACG GAGGGGCCAA GGGGCAGCTG 
GGCTCCCAGC TCTCACTGAG CCAGCAGCAG ACGCCCGGCT CGCCCCCACG GTTGGCCGTT 
CACAAAAGCA CACAGGCGCT GCCCACCAGC AGCTTCAGCC AGGATTCACG GCCTCTATCG 
GCTTCGCAGC CCTCCCTGCC CCACGGGCTG GCGGCTGGCA GCACCCAGAG CCCCCCGGCC 
TCAGTGCACG AATCCTCTGC TTCCATCGGT GGAGGCCCCG CCGCCACCGC CGTCTCGCCG 
GGTTCGGGTC CCCCAGCCGG GCTGCGGGGC GCAGTGTCCT CGAGGGGGCC CCTGTCTCAC 
CCTGCGCCCA CCCTGCAGCA GGACTCGGCG GCAGCCCGCA AGGATTCGGT GGGCAGCATG 
CCCGACACGG ACCCAGCCAA ATCCAGGCTG TCTTCCAACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN2 ( XM_003642867.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003642915.1 Gallus gallus hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2), mRNA.

Price & Availability↑

CloneID	OGa20478
Clone ID Related Accession (Same CDS sequence)	XM_003642867.3
Accession Version	XM_003642867.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003642867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCGG GCGGGGAGGC GGCGGCAGAG GAGGAGGCGG CCGAAGGGGC CAAACGCGGC 
GGAGGGGCGG CCGCGGGGAG AGCGCGGGGC CGCGGGGGGA AAGGATCCCC GAACGGGGAG 
TGCCGCCGCG GGGAAACGCC GCGCAGCCCC AGCGCGGAGC AGCCCCGGGA GCCGAAGGTC 
TCCTTCTCCT GCGGCGGTGG CGGCGGCGGC GGCGGCGGCG GCAGCGCATC CCCAGGCGGG 
GCCAAGGCGG CCGAGGAAGG CGACGATGCG GGCGAGGAGG TTCGCGGGAG CCAGGCCAGC 
TTCATGCAGA GGCAGTTCGG GGCCATGCTC CAGCCCGGCG TCAACAAGTT CTCGCTGCGG 
ATGTTCGGTT CCCAGAAGGC GGTGGAACGG GAGCAGGAAC GCGTTAAGTC AGCGGGGGCC 
TGGATCATCC ATCCCTACAG CGATTTCAGG TTCTACTGGG ACTTCACGAT GCTGCTCTTC 
ATGGTGGGCA ACCTGATCAT CATTCCCGTG GGCATCACCT TCTTCAAGGA GGAAACCACG 
GCGCCCTGGA TCGTCTTCAA CGTGGTCTCC GACACCTTCT TCCTGATGGA CCTGGTGCTG 
AACTTCCGCA CGGGGATCGT CATCGAGGAC AACACCGAGA TCATCCTGGA CCCCGAAAGG 
ATCAAGAAGA AGTACCTGAA GACGTGGTTT GTGGTGGACT TCGTCTCCTC CATCCCCGTG 
GACTACGTCT TCCTCATTGT GGAGAAGGGC ATCGACTCGG AGGTCTATAA GACAGCCCGT 
GCTCTCCGCA TCGTCCGCTT CACCAAGATC CTCAGCCTGC TGCGCCTGCT GCGCCTCTCC 
CGCCTCATCC GCTACATCCA CCAGTGGGAG GAGATCTTCC ACATGACCTA CGACCTGGCC 
AGTGCCGTGA TGAGGATCAT TAACCTCATT GGCATGATGC TGCTGCTGTG CCACTGGGAC 
GGCTGTCTCC AGTTCCTCGT GCCCATGCTG CAGGATTTCC CCCAGAACTG CTGGGTCTCC 
ATCAATGGGA TGGTGAACGA CTCCTGGAGC GAGCTGTACT CCTTCGCCCT CTTCAAGTCC 
ATGAGCCACA TGCTGTGCAT CGGCTACGGC AAGCAGGCGC CCGAGAGCAT GACGGACATC 
TGGCTGACGA TGCTGAGCAT GATCGTGGGA GCCACCTGCT ATGCCATGTT CATCGGCCAC 
GCCACCGCCC TCATCCAGTC TCTGGACTCC TCCCGGCGCC AGTATCAGGA GAAGTACAAG 
CAGGTGGAGC AGTACATGTC CTTCCACAAG CTGCCCGCTG ACTTCAGACA GAAGATCCAC 
GACTACTACG AGCACCGCTA CCAGGGCAAG ATGTTCGACG AGGACAGCAT CCTTGGGGAG 
CTCAACGAGC CCTTACGTGA GGAAATCGTG AACTTCAACT GCCGCAAACT GGTGGCCTCC 
ATGCCTCTGT TTGCCAACGC GGACCCCAAC TTCGTGACGG CGATGCTCAC CAAGCTGAAG 
TTTGAGGTGT TCCAGCCAGG CGACTACATC ATCCGCGAGG GGACCATCGG CAAGAAGATG 
TACTTCATCC AGCACGGCGT GGTCAGCATC CTCACCAAGG GCAACAAGGA GATGAAGCTC 
TCTGATGGCT CCTACTTCGG GGAAATCTGC CTGCTGACCC GTGGTCGGCG CACAGCCAGC 
GTCCGGGCGG ACACCTACTG CCGCCTCTAC TCGCTGTCAG TGGACAACTT CAACGAGGTG 
CTGGAGGAGT ACCCCATGAT GAGGAGAGCC TTTGAGACCG TGGCCATTGA CCGCCTTGAT 
CGCATCGGGA AAAAGAACTC GATCCTTCTT CACAAAGTGC AGCACGACCT CAACTCGGGC 
GTCTTCAACA ACCAGGAGAA CGAAATCATC CAGGAGATTG TCAAGTATGA CCGAGAGATG 
GTCCAGCAGG CGGAGCTGCA GCAGCACACG GCCATGTACA GCCCGGTGCA ACCCCAGGTC 
ACCTCTGCCA TTGCCACCTT GCAGCAAGCC GTCGCCATGA GCTTCTGCCC ACAGATGGCC 
AGCCCGCTGG TGGGCTCCAT GGCTCTGGGC TCGCCCCGCA TGATGCGTCG CTTGCAATAC 
GCCCAGGCCG TGCCCAGCCC CTTCGCCGTC TCCCCAGTGC TGCTGCAGCA GAGCCCCCCG 
CAGCAGCCGC AGCCCCCCGT GCCCCACGCC AACCCATCGC CCTCGCAGGA CCAGGCACAG 
CCCGCCTCCG CCAGCGCCTT CGCTGCTGCC AGCCCGCCGT CCCAAAGCCC CTTGGCCAGC 
CGGACGTTTG CCTACGGAGG GGCCAAGGGG CAGCTGGGCT CCCAGCTCTC ACTGAGCCAG 
CAGCAGACGC CCGGCTCGCC CCCACGGTTG GCCGTTCACA AAAGCACACA GGCGCTGCCC 
ACCAGCAGCT TCAGCCAGGA TTCACGGCCT CTATCGGCTT CGCAGCCCTC CCTGCCCCAC 
GGGCTGGCGG CTGGCAGCAC CCAGAGCCCC CCGGCCTCAG TGCACGAATC CTCTGCTTCC 
ATCGGTGGAG GCCCCGCCGC CACCGCCGTC TCGCCGGGTT CGGGTCCCCC AGCCGGGCTG 
CGGGGCGCAG TGTCCTCGAG GGGGCCCCTG TCTCACCCTG CGCCCACCCT GCAGCAGGAC 
TCGGCGGCAG CCCGCAAGGA TTCGGTGGGC AGCATGCCCG ACACGGACCC AGCCAAATCC 
AGGCTGTCTT CCAACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003642915.1
CDS	77..2797
Translation	MRSGGEAAAEEEAAEGAKRGGGAAAGRARGRGGKGSPNGECRRGETPRSPSAEQPREPKVSFSCGGGGGGGGGGSASPGGAKAAEEGDDAGEEVRGSQASFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKEETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPERIKKKYLKTWFVVDFVSSIPVDYVFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRIINLIGMMLLLCHWDGCLQFLVPMLQDFPQNCWVSINGMVNDSWSELYSFALFKSMSHMLCIGYGKQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNEPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSILTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENEIIQEIVKYDREMVQQAELQQHTAMYSPVQPQVTSAIATLQQAVAMSFCPQMASPLVGSMALGSPRMMRRLQYAQAVPSPFAVSPVLLQQSPPQQPQPPVPHANPSPSQDQAQPASASAFAAASPPSQSPLASRTFAYGGAKGQLGSQLSLSQQQTPGSPPRLAVHKSTQALPTSSFSQDSRPLSASQPSLPHGLAAGSTQSPPASVHESSASIGGGPAATAVSPGSGPPAGLRGAVSSRGPLSHPAPTLQQDSAAARKDSVGSMPDTDPAKSRLSSNL

Target ORF information:

RefSeq Version	XM_003642867.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (HCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003642867.3

ORF Insert Sequence:

ATGCGATCGG GCGGGGAGGC GGCGGCAGAG GAGGAGGCGG CCGAAGGGGC CAAACGCGGC 
GGAGGGGCGG CCGCGGGGAG AGCGCGGGGC CGCGGGGGGA AAGGATCCCC GAACGGGGAG 
TGCCGCCGCG GGGAAACGCC GCGCAGCCCC AGCGCGGAGC AGCCCCGGGA GCCGAAGGTC 
TCCTTCTCCT GCGGCGGTGG CGGCGGCGGC GGCGGCGGCG GCAGCGCATC CCCAGGCGGG 
GCCAAGGCGG CCGAGGAAGG CGACGATGCG GGCGAGGAGG TTCGCGGGAG CCAGGCCAGC 
TTCATGCAGA GGCAGTTCGG GGCCATGCTC CAGCCCGGCG TCAACAAGTT CTCGCTGCGG 
ATGTTCGGTT CCCAGAAGGC GGTGGAACGG GAGCAGGAAC GCGTTAAGTC AGCGGGGGCC 
TGGATCATCC ATCCCTACAG CGATTTCAGG TTCTACTGGG ACTTCACGAT GCTGCTCTTC 
ATGGTGGGCA ACCTGATCAT CATTCCCGTG GGCATCACCT TCTTCAAGGA GGAAACCACG 
GCGCCCTGGA TCGTCTTCAA CGTGGTCTCC GACACCTTCT TCCTGATGGA CCTGGTGCTG 
AACTTCCGCA CGGGGATCGT CATCGAGGAC AACACCGAGA TCATCCTGGA CCCCGAAAGG 
ATCAAGAAGA AGTACCTGAA GACGTGGTTT GTGGTGGACT TCGTCTCCTC CATCCCCGTG 
GACTACGTCT TCCTCATTGT GGAGAAGGGC ATCGACTCGG AGGTCTATAA GACAGCCCGT 
GCTCTCCGCA TCGTCCGCTT CACCAAGATC CTCAGCCTGC TGCGCCTGCT GCGCCTCTCC 
CGCCTCATCC GCTACATCCA CCAGTGGGAG GAGATCTTCC ACATGACCTA CGACCTGGCC 
AGTGCCGTGA TGAGGATCAT TAACCTCATT GGCATGATGC TGCTGCTGTG CCACTGGGAC 
GGCTGTCTCC AGTTCCTCGT GCCCATGCTG CAGGATTTCC CCCAGAACTG CTGGGTCTCC 
ATCAATGGGA TGGTGAACGA CTCCTGGAGC GAGCTGTACT CCTTCGCCCT CTTCAAGTCC 
ATGAGCCACA TGCTGTGCAT CGGCTACGGC AAGCAGGCGC CCGAGAGCAT GACGGACATC 
TGGCTGACGA TGCTGAGCAT GATCGTGGGA GCCACCTGCT ATGCCATGTT CATCGGCCAC 
GCCACCGCCC TCATCCAGTC TCTGGACTCC TCCCGGCGCC AGTATCAGGA GAAGTACAAG 
CAGGTGGAGC AGTACATGTC CTTCCACAAG CTGCCCGCTG ACTTCAGACA GAAGATCCAC 
GACTACTACG AGCACCGCTA CCAGGGCAAG ATGTTCGACG AGGACAGCAT CCTTGGGGAG 
CTCAACGAGC CCTTACGTGA GGAAATCGTG AACTTCAACT GCCGCAAACT GGTGGCCTCC 
ATGCCTCTGT TTGCCAACGC GGACCCCAAC TTCGTGACGG CGATGCTCAC CAAGCTGAAG 
TTTGAGGTGT TCCAGCCAGG CGACTACATC ATCCGCGAGG GGACCATCGG CAAGAAGATG 
TACTTCATCC AGCACGGCGT GGTCAGCATC CTCACCAAGG GCAACAAGGA GATGAAGCTC 
TCTGATGGCT CCTACTTCGG GGAAATCTGC CTGCTGACCC GTGGTCGGCG CACAGCCAGC 
GTCCGGGCGG ACACCTACTG CCGCCTCTAC TCGCTGTCAG TGGACAACTT CAACGAGGTG 
CTGGAGGAGT ACCCCATGAT GAGGAGAGCC TTTGAGACCG TGGCCATTGA CCGCCTTGAT 
CGCATCGGGA AAAAGAACTC GATCCTTCTT CACAAAGTGC AGCACGACCT CAACTCGGGC 
GTCTTCAACA ACCAGGAGAA CGAAATCATC CAGGAGATTG TCAAGTATGA CCGAGAGATG 
GTCCAGCAGG CGGAGCTGCA GCAGCACACG GCCATGTACA GCCCGGTGCA ACCCCAGGTC 
ACCTCTGCCA TTGCCACCTT GCAGCAAGCC GTCGCCATGA GCTTCTGCCC ACAGATGGCC 
AGCCCGCTGG TGGGCTCCAT GGCTCTGGGC TCGCCCCGCA TGATGCGTCG CTTGCAATAC 
GCCCAGGCCG TGCCCAGCCC CTTCGCCGTC TCCCCAGTGC TGCTGCAGCA GAGCCCCCCG 
CAGCAGCCGC AGCCCCCCGT GCCCCACGCC AACCCATCGC CCTCGCAGGA CCAGGCACAG 
CCCGCCTCCG CCAGCGCCTT CGCTGCTGCC AGCCCGCCGT CCCAAAGCCC CTTGGCCAGC 
CGGACGTTTG CCTACGGAGG GGCCAAGGGG CAGCTGGGCT CCCAGCTCTC ACTGAGCCAG 
CAGCAGACGC CCGGCTCGCC CCCACGGTTG GCCGTTCACA AAAGCACACA GGCGCTGCCC 
ACCAGCAGCT TCAGCCAGGA TTCACGGCCT CTATCGGCTT CGCAGCCCTC CCTGCCCCAC 
GGGCTGGCGG CTGGCAGCAC CCAGAGCCCC CCGGCCTCAG TGCACGAATC CTCTGCTTCC 
ATCGGTGGAG GCCCCGCCGC CACCGCCGTC TCGCCGGGTT CGGGTCCCCC AGCCGGGCTG 
CGGGGCGCAG TGTCCTCGAG GGGGCCCCTG TCTCACCCTG CGCCCACCCT GCAGCAGGAC 
TCGGCGGCAG CCCGCAAGGA TTCGGTGGGC AGCATGCCCG ACACGGACCC AGCCAAATCC 
AGGCTGTCTT CCAACTTGTG A