TLR4 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR4
Entrez Gene ID	417241
Full Name	toll like receptor 4
General protein information	Preferred Names toll like receptor 4 Names toll-like receptor 4
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030693.1	NP_001025864.1	toll-like receptor 4 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tlr4	NP_067272.1
Danio rerio (zebrafish)	tlr4ba	NP_001124523.1
Pan troglodytes (chimpanzee)	TLR4	NP_001138335.1
Gallus gallus (chicken)	TLR4	NP_001025864.1
Homo sapiens (human)	TLR4	NP_612564.1
	TLR4	NP_001032169.1
Rattus norvegicus (Norway rat)	Tlr4	NP_062051.1
Danio rerio (zebrafish)	tlr4al	XP_001919699.4
Danio rerio (zebrafish)	tlr4bb	NP_997978.1
Canis lupus familiaris (dog)	TLR4	NP_001002950.2
Bos taurus (cattle)	TLR4	NP_776623.5

Gene Ontology
Bibliography

Related articles in PubMed

TLR4 and TLR21 expression, MIF, IFN-?, MD-2, CD14 activation, and sIgA production in chickens administered with EFAL41 strain challenged with Campylobacter jejuni.
V K, E M, K B, R H, V R, D S, A K, M L, M L Jr, A L, Z ?, M L Sr
Folia microbiologica62(2)89-97(2017 Mar)

Effects of lipopolysaccharide on the histomorphology and expression of toll-like receptor 4 in the chicken trachea and lung.
Ansari AR, Ge XH, Huang HB, Huang XY, Zhao X, Peng KM, Zhong JM, Liu HZ
Avian pathology : journal of the W.V.P.A45(5)530-7(2016 Oct)

Difference in resistance to Salmonella enteritidis infection among allelic variants of TLR4 (903, 1832) in SPF chickens.
Liu L, Zhao XW, Song YM, Li QH, Li P, Liu RR, Zheng MQ, Wen J, Zhao GP
Journal of applied genetics57(3)389-96(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Enterococcus faecium probiotic protects chickens from Campylobacter jejuni infection by modulating the expression of TKR4, TLR21, and immune response proteins.
Title: TLR4 and TLR21 expression, MIF, IFN-?, MD-2, CD14 activation, and sIgA production in chickens administered with EFAL41 strain challenged with Campylobacter jejuni.

genetic variants in TLR4 loci 903 and 1832 are associated with resistance to Salmonella enteritidis infection in SPF White Leghorns
Title: Difference in resistance to Salmonella enteritidis infection among allelic variants of TLR4 (903, 1832) in SPF chickens.

The TLR4 gene is polymorphic among different chicken breeds.
Title: Polymorphisms of chicken TLR3 and 7 in different breeds.

Results show that the Toll-like receptors TLR4 and TLR7 that the mRNA expression levels in the spleen were lower in the 300, 600, and 900 mg/kg groups than those in the control group.
Title: Downregulation of TLR4 and 7 mRNA expression levels in broiler's spleen caused by diets supplemented with nickel chloride.

TLR4 is constitutively expressed by epithelial cells in the thymus, suggesting it may participate in thymic development by inducing factors affecting its development.
Title: TLR4 is constitutively expressed in chick thymic epithelial cells.

The following TLR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00258	NM_001030693.1 Latest version!	Gallus gallus toll like receptor 4 (TLR4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR4 ( NM_001030693.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001025864.1 Gallus gallus toll like receptor 4 (TLR4), mRNA.

Price & Availability↑

CloneID	OGa00258
Clone ID Related Accession (Same CDS sequence)	NM_001030693.1
Accession Version	NM_001030693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Gallus gallus(chicken)
Product	toll-like receptor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY064697.1. On Aug 8, 2005 this sequence version replaced XM_415518.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JQ711146.1, DR426681.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCCAGCA GAGCGGCTCC CACCGCGCTG ACTCTTGGGG TGCTGCTGCA GCTGCTGCTC 
GTGCTGTCCC TGCTGGCAGG ATGCATCCCC AGTCCGTGCC TGGAGGTCAT CCCCAGCACA 
GCTTTCAGAT GCACAGGACA GAACATCTCT GGAGTTCCTG CTGAAATCCC AAACACCACC 
CTGGACTTGG ACCTCAGTTT CAACAGTCTG AAATTGCTGA GCTCAAATTA TTTTTCATCA 
GTACCCGAAC TGCAGTTTCT GGATCTTTCA AGGTGCCACA TCCATACAAT AGAAGATAAC 
TCTTTTGTGG ATCTTTATAA CCTTTCCACC TTAATTTTAA CTGCCAATTC ACTCCAGCAC 
CTGGGTCTAG CAGCCTTCCA TGGCTTAACG TCGCTGAAAA AACTAGTACT GGTGGAAACA 
AGCATATCCT CTCTGTCTGA CCTACCCATC GGACACTTGA ATACCCTGCA GGAGCTGAAT 
CTGGGCCATA ACAACATTGC TTCATTGAAG CTTCCTAAGT ATTTTGCCAA CCTGACCTCT 
CTCAGGCACC TGAGCTTTTC CTCCAATAAT ATTACATATA TCTCCAAAGG AGACCTTGAT 
GCCCTGAGGG AAACAAACAG GCTCAACCTC ACGTTGGTAC TTTCTCTGAA CAATATAAAA 
TACATCCAGT CAGGATCCTT TGCAAAGATT CACCTTGGTG AACTGATTCT GAGATCCTCT 
TTTGAGAACC TCAATGCGAT GCACTCTTCT CTTCAGGGCC TGGCAGGTTT ACAGGTCAAC 
AGACTAATAG TTGGAGAATT CACTAACATT CTGAAAATAA CGGCATTTCA GAACGGACTC 
TTGAGTGGAC TGTGTCAGGT ACAGATGCAG GAGTTTGTCT TAATGTGTTT CAGAGAGTTT 
GAGAATGACA CGGACACTCT TTTTGACTGC ATAGGCAACG TCACCACTAT TCGGTTGGTG 
GACCTGAATC TTGAAACGTT GTCAGAGGTT CCTATGTTTT CTCAAGTGAA ACATCTGGAA 
TGGAAGAGAT GTAAGTTTCA GGAATTGCCT GCTGAGAAGC TGTCTCTTTT TAAGGAGTTG 
AGAGTGCTTC GTATTACCAA GAGCAAAGAC CTAAATGGCT TCGAGCAAAA GTTTGGGAGT 
CTAACTTACC TGGAGGTTGT AGATTTGAGT GAGAATCGTC TCTCCTTCCT TACCTGCTGT 
TCCCCTAAGT TTCCCAGGTC TCCAAATTTG AAACACTTGA ATCTAAGCTT CAATTCTGAC 
ATCAGTTTGA CTGGAGAATT TGCTAATCTG AGAAATCTGC TATACTTGGA CCTTCAACAC 
ACAAAGCTAA TTCATCATGG CACCTACCCT GTCTTTCTCC TCCTTCAGAA ACTCATTTAC 
CTTGATATCT CCTACACCAA AACTCATGTT ATGTCCCATC TCATCTTTCA TGGCTTGAAC 
TCTTTGCAAG TGCTGAAGAT GGCAGGCAAC TCCTTTGAGA ACAATACATT GACCAACAAC 
TTTGAAAATG TAAGGCGGCT CCGCATCTTG GATATTTCAA GTTGCAAATT AGTATGGGTG 
GACCAAAGTA CATTTAATGC TCTCTCTGAA CTAAAAGAGC TGATCATCAG CAACAACAAG 
CTATTGACTT TTGATCCTGT AACATACAAG CCACTCCAAG CCCTCACAGC TCTGGATTTC 
AGCAACAACC AGATGAGTTT CCTGTCGGAC TCAGCCCTGG AAATCCTGCC TGACAGTCTG 
GTCTTGCTAG ACATTTCTCA CAACTTGTTT GAATGCTCTT GCACACATTT GAACTTCCTG 
AAATGGGTCA AGGAAAAGCA GGATCTACTG CAGAACAAGC ATTCAATGAT ATGCCACACA 
CCTGCCTACA TGAAGAACAT GAGCCTGTCA AACTTTGATA TGTCCTCTTG CCATCCCAAC 
CCAACCACAG TAGCATGCTC AGTGACTGTG TTGCTTGCTG CAGGAGTGTT TCTATTCCTC 
ATTTATAAGT ACTACTTCCA GCTATACTAC TCATTGGTGC TGCTCAGTGG GTGTAAACAC 
TCTGCAGAAA GGGGAGATAT CTATGATGCC TTTGTTATCC ATTCCAGCAA AGACCAAGAA 
TGGGTGATGA AAGAGCTGGT GGAACCCTTA GAAGAAGGAA AACCTCCCTT CCAGCTTTGT 
CTTTACTTCA GGGATTTTTT ACCAGGGGTA CCCATTGTCA CTAATATAAT CCAAGAAGGT 
TTTCTGAGTA GCAGAAATGT CATTGCAGTC ATCTCAGCTG ACTTTCTGGA AAGCAAGTGG 
TGTAGCTTTG AGTTTGACAT TGCTCGGTCC TGGCAGCTTG TTGAAGGGAA GGCTGGAATA 
ATCATGATCA TCCTAGGGGA AGTGGATAAG ACCTTGCTGA GGCAGAGGCT GGGACTGTCC 
CGATATCTGA GGAGGAACAC TTATCTGGAG TGGAAAAACA AGGAAATAAG CAGGCATATC 
TTCTGGAGGC AGCTGACATC AGTCCTGCTA GAAGGCAAAA AATGGAATCA CGAGGAGATA 
AAACTCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025864.1
CDS	1..2532
Translation	MPSRAAPTALTLGVLLQLLLVLSLLAGCIPSPCLEVIPSTAFRCTGQNISGVPAEIPNTTLDLDLSFNSLKLLSSNYFSSVPELQFLDLSRCHIHTIEDNSFVDLYNLSTLILTANSLQHLGLAAFHGLTSLKKLVLVETSISSLSDLPIGHLNTLQELNLGHNNIASLKLPKYFANLTSLRHLSFSSNNITYISKGDLDALRETNRLNLTLVLSLNNIKYIQSGSFAKIHLGELILRSSFENLNAMHSSLQGLAGLQVNRLIVGEFTNILKITAFQNGLLSGLCQVQMQEFVLMCFREFENDTDTLFDCIGNVTTIRLVDLNLETLSEVPMFSQVKHLEWKRCKFQELPAEKLSLFKELRVLRITKSKDLNGFEQKFGSLTYLEVVDLSENRLSFLTCCSPKFPRSPNLKHLNLSFNSDISLTGEFANLRNLLYLDLQHTKLIHHGTYPVFLLLQKLIYLDISYTKTHVMSHLIFHGLNSLQVLKMAGNSFENNTLTNNFENVRRLRILDISSCKLVWVDQSTFNALSELKELIISNNKLLTFDPVTYKPLQALTALDFSNNQMSFLSDSALEILPDSLVLLDISHNLFECSCTHLNFLKWVKEKQDLLQNKHSMICHTPAYMKNMSLSNFDMSSCHPNPTTVACSVTVLLAAGVFLFLIYKYYFQLYYSLVLLSGCKHSAERGDIYDAFVIHSSKDQEWVMKELVEPLEEGKPPFQLCLYFRDFLPGVPIVTNIIQEGFLSSRNVIAVISADFLESKWCSFEFDIARSWQLVEGKAGIIMIILGEVDKTLLRQRLGLSRYLRRNTYLEWKNKEISRHIFWRQLTSVLLEGKKWNHEEIKLM

Target ORF information:

RefSeq Version	NM_001030693.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll like receptor 4 (TLR4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030693.1

ORF Insert Sequence:

ATGCCCAGCA GAGCGGCTCC CACCGCGCTG ACTCTTGGGG TGCTGCTGCA GCTGCTGCTC 
GTGCTGTCCC TGCTGGCAGG ATGCATCCCC AGTCCGTGCC TGGAGGTCAT CCCCAGCACA 
GCTTTCAGAT GCACAGGACA GAACATCTCT GGAGTTCCTG CTGAAATCCC AAACACCACC 
CTGGACTTGG ACCTCAGTTT CAACAGTCTG AAATTGCTGA GCTCAAATTA TTTTTCATCA 
GTACCCGAAC TGCAGTTTCT GGATCTTTCA AGGTGCCACA TCCATACAAT AGAAGATAAC 
TCTTTTGTGG ATCTTTATAA CCTTTCCACC TTAATTTTAA CTGCCAATTC ACTCCAGCAC 
CTGGGTCTAG CAGCCTTCCA TGGCTTAACG TCGCTGAAAA AACTAGTACT GGTGGAAACA 
AGCATATCCT CTCTGTCTGA CCTACCCATC GGACACTTGA ATACCCTGCA GGAGCTGAAT 
CTGGGCCATA ACAACATTGC TTCATTGAAG CTTCCTAAGT ATTTTGCCAA CCTGACCTCT 
CTCAGGCACC TGAGCTTTTC CTCCAATAAT ATTACATATA TCTCCAAAGG AGACCTTGAT 
GCCCTGAGGG AAACAAACAG GCTCAACCTC ACGTTGGTAC TTTCTCTGAA CAATATAAAA 
TACATCCAGT CAGGATCCTT TGCAAAGATT CACCTTGGTG AACTGATTCT GAGATCCTCT 
TTTGAGAACC TCAATGCGAT GCACTCTTCT CTTCAGGGCC TGGCAGGTTT ACAGGTCAAC 
AGACTAATAG TTGGAGAATT CACTAACATT CTGAAAATAA CGGCATTTCA GAACGGACTC 
TTGAGTGGAC TGTGTCAGGT ACAGATGCAG GAGTTTGTCT TAATGTGTTT CAGAGAGTTT 
GAGAATGACA CGGACACTCT TTTTGACTGC ATAGGCAACG TCACCACTAT TCGGTTGGTG 
GACCTGAATC TTGAAACGTT GTCAGAGGTT CCTATGTTTT CTCAAGTGAA ACATCTGGAA 
TGGAAGAGAT GTAAGTTTCA GGAATTGCCT GCTGAGAAGC TGTCTCTTTT TAAGGAGTTG 
AGAGTGCTTC GTATTACCAA GAGCAAAGAC CTAAATGGCT TCGAGCAAAA GTTTGGGAGT 
CTAACTTACC TGGAGGTTGT AGATTTGAGT GAGAATCGTC TCTCCTTCCT TACCTGCTGT 
TCCCCTAAGT TTCCCAGGTC TCCAAATTTG AAACACTTGA ATCTAAGCTT CAATTCTGAC 
ATCAGTTTGA CTGGAGAATT TGCTAATCTG AGAAATCTGC TATACTTGGA CCTTCAACAC 
ACAAAGCTAA TTCATCATGG CACCTACCCT GTCTTTCTCC TCCTTCAGAA ACTCATTTAC 
CTTGATATCT CCTACACCAA AACTCATGTT ATGTCCCATC TCATCTTTCA TGGCTTGAAC 
TCTTTGCAAG TGCTGAAGAT GGCAGGCAAC TCCTTTGAGA ACAATACATT GACCAACAAC 
TTTGAAAATG TAAGGCGGCT CCGCATCTTG GATATTTCAA GTTGCAAATT AGTATGGGTG 
GACCAAAGTA CATTTAATGC TCTCTCTGAA CTAAAAGAGC TGATCATCAG CAACAACAAG 
CTATTGACTT TTGATCCTGT AACATACAAG CCACTCCAAG CCCTCACAGC TCTGGATTTC 
AGCAACAACC AGATGAGTTT CCTGTCGGAC TCAGCCCTGG AAATCCTGCC TGACAGTCTG 
GTCTTGCTAG ACATTTCTCA CAACTTGTTT GAATGCTCTT GCACACATTT GAACTTCCTG 
AAATGGGTCA AGGAAAAGCA GGATCTACTG CAGAACAAGC ATTCAATGAT ATGCCACACA 
CCTGCCTACA TGAAGAACAT GAGCCTGTCA AACTTTGATA TGTCCTCTTG CCATCCCAAC 
CCAACCACAG TAGCATGCTC AGTGACTGTG TTGCTTGCTG CAGGAGTGTT TCTATTCCTC 
ATTTATAAGT ACTACTTCCA GCTATACTAC TCATTGGTGC TGCTCAGTGG GTGTAAACAC 
TCTGCAGAAA GGGGAGATAT CTATGATGCC TTTGTTATCC ATTCCAGCAA AGACCAAGAA 
TGGGTGATGA AAGAGCTGGT GGAACCCTTA GAAGAAGGAA AACCTCCCTT CCAGCTTTGT 
CTTTACTTCA GGGATTTTTT ACCAGGGGTA CCCATTGTCA CTAATATAAT CCAAGAAGGT 
TTTCTGAGTA GCAGAAATGT CATTGCAGTC ATCTCAGCTG ACTTTCTGGA AAGCAAGTGG 
TGTAGCTTTG AGTTTGACAT TGCTCGGTCC TGGCAGCTTG TTGAAGGGAA GGCTGGAATA 
ATCATGATCA TCCTAGGGGA AGTGGATAAG ACCTTGCTGA GGCAGAGGCT GGGACTGTCC 
CGATATCTGA GGAGGAACAC TTATCTGGAG TGGAAAAACA AGGAAATAAG CAGGCATATC 
TTCTGGAGGC AGCTGACATC AGTCCTGCTA GAAGGCAAAA AATGGAATCA CGAGGAGATA 
AAACTCATGT AA