T cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	T
Entrez Gene ID	395782
Full Name	T brachyury transcription factor
Synonyms	CH-T
General protein information	Preferred Names T brachyury transcription factor Names brachyury protein T, brachyury homolog brachyury (T) protein T
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204940.2	NP_990271.2	brachyury protein
XM_015284045.2	XP_015139531.2	brachyury protein isoform X1
XM_015284045.1	XP_015139531.1	brachyury protein isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	T	NP_001003092.1
Rattus norvegicus (Norway rat)	T	NP_001099679.1
Gallus gallus (chicken)	T	NP_990271.1
Danio rerio (zebrafish)	LOC100004296	XP_001343633.3
Mus musculus (house mouse)	T	NP_033335.1
Homo sapiens (human)	T	NP_003172.1
	T	XP_001101514.1
Pan troglodytes (chimpanzee)	T	XP_527563.3
Bos taurus (cattle)	T	NP_001179914.1
Xenopus tropicalis (tropical clawed frog)	t	NP_001008139.1

Gene Ontology
Bibliography

Related articles in PubMed

Two novel chick T-box genes related to mouse Brachyury are expressed in different, non-overlapping mesodermal domains during gastrulation.
Knezevic V, De Santo R, Mackem S
Development (Cambridge, England)124(2)411-9(1997 Jan)

The chick Brachyury gene: developmental expression pattern and response to axial induction by localized activin.
Kispert A, Ortner H, Cooke J, Herrmann BG
Developmental biology168(2)406-15(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following T gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the T cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa28381	NM_204940.2 Latest version!	Gallus gallus T, brachyury homolog (mouse) (T), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OGa148575	XM_015284045.2 Latest version!	Gallus gallus T brachyury transcription factor (T), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OGa33577	XM_015284045.1 Latest version!	Gallus gallus T, brachyury homolog (mouse) (T), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

T ( NM_204940.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990271.2 Gallus gallus T, brachyury homolog (mouse) (T), mRNA.

Price & Availability↑

CloneID	OGa28381
Clone ID Related Accession (Same CDS sequence)	NM_204940.2
Accession Version	NM_204940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-26
Organism	Gallus gallus(chicken)
Product	brachyury protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN03003030.1. On Jul 10, 2015 this sequence version replaced NM_204940.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: U25176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SRS352357, SRS352358 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCCC CGGAGGACGC GGGCAAGGCG CCGGCGTACC GAGTGGACCA CCTGCTGAGC 
GCGGTGGAGA GCGAGCTGCA GGCGGGCAGC GAGAAGGGAG ACCCGACGGA GCGCGAGCTG 
CGCGTGGCGC TGGAGGACGG CGAGCTGTGG CTGCGCTTCA AGGAGCTCAC CAACGAGATG 
ATCGTCACCA AGAACGGCAG GAGGATGTTC CCGGTGCTGA AGGTGAGCGT GTCGGGGCTG 
GACCCCAACG CCATGTACTC CTTCCTGCTG GACTTCGTGG CGGCCGACGG GCACCGCTGG 
AAGTACGTCA ACGGCGAGTG GGTGCCGGGC GGCAAGCCGG AGCCGCAGGC GCCCAGCTGC 
GTCTACATCC ACCCCGACTC GCCCAACTTC GGCGCCCACT GGATGAAGGC GCCCGTCTCC 
TTCAGCAAGG TCAAGCTCAC CAACAAGCTC AACGGCGGCG GGCAGATCAT GCTCAACTCC 
CTGCACAAGT ACGAGCCCCG CATCCACATC GTGCGGGTGG GCGGCCCGCA GCGGATGATC 
ACCAGCCATT CCTTCCCCGA GACCCAGTTC ATCGCTGTGA CGGCGTACCA GAACGAGGAG 
ATCACAGCTT TAAAAATTAA ATACAACCCA TTTGCGAAGG CATTTCTTGA CGCAAAGGAA 
AGAAATGATC ACAAAGACAT GATGGAGGAA GCGGGAGACA ACCAGCAGTC TGGATACTCG 
CAGTTAGGTA GTTGGCTTAT TCCCGGGACT GGGGCTCTGT GCCCACCTGC CAATCCTCAC 
TCTCAGTTTG GAGCACCCCT GTCTCTCTCC CCTGCTCACA GCTGTGAAAG GTACTCACCG 
CTGAGGAATC ACCGTTCTGC CCCCTACCCC AATCCCTACA CCCATAGAAA CAACTCACCA 
ACAGCTTACA CTGATAACTC CTCTGCCTGC CTTCCCATGC TCCAGTCCCA TGACAATTGG 
TCCTCCCTGG GAGTTCCCAC ACACACGACT ATGCTGCCCA TGAGCCACAG CACTGGCACG 
GCTACCAGCT CCAGTCAGTA CCCGAACTTA TGGTCCGTGA GTAACAGCAC CATCACCCCG 
GCGCCTCAGT CAAGCGGGAT GTCCAACGGC CTGAGCTCCC AGTTCCTGCG TGGCTCTCCG 
GTGCACTACA CCGCTCTCCC ACATCCCGTC ACTGCCACCA CCTCCACATC CCCACTGTAC 
GATGGAGGAG CACCTGCAGA CCTTCCTGAC AGCCAGTACG ATGCCTCTGC ACACACTCGG 
TTGGCATCCA TGTGGACGCC CATCACCCCA CCTTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990271.2
CDS	72..1373
Translation	MGSPEDAGKAPAYRVDHLLSAVESELQAGSEKGDPTERELRVALEDGELWLRFKELTNEMIVTKNGRRMFPVLKVSVSGLDPNAMYSFLLDFVAADGHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHSFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERNDHKDMMEEAGDNQQSGYSQLGSWLIPGTGALCPPANPHSQFGAPLSLSPAHSCERYSPLRNHRSAPYPNPYTHRNNSPTAYTDNSSACLPMLQSHDNWSSLGVPTHTTMLPMSHSTGTATSSSQYPNLWSVSNSTITPAPQSSGMSNGLSSQFLRGSPVHYTALPHPVTATTSTSPLYDGGAPADLPDSQYDASAHTRLASMWTPITPPSM

Target ORF information:

RefSeq Version	NM_204940.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus T, brachyury homolog (mouse) (T), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204940.2

ORF Insert Sequence:

ATGGGCTCCC CGGAGGACGC GGGCAAGGCG CCGGCGTACC GAGTGGACCA CCTGCTGAGC 
GCGGTGGAGA GCGAGCTGCA GGCGGGCAGC GAGAAGGGAG ACCCGACGGA GCGCGAGCTG 
CGCGTGGCGC TGGAGGACGG CGAGCTGTGG CTGCGCTTCA AGGAGCTCAC CAACGAGATG 
ATCGTCACCA AGAACGGCAG GAGGATGTTC CCGGTGCTGA AGGTGAGCGT GTCGGGGCTG 
GACCCCAACG CCATGTACTC CTTCCTGCTG GACTTCGTGG CGGCCGACGG GCACCGCTGG 
AAGTACGTCA ACGGCGAGTG GGTGCCGGGC GGCAAGCCGG AGCCGCAGGC GCCCAGCTGC 
GTCTACATCC ACCCCGACTC GCCCAACTTC GGCGCCCACT GGATGAAGGC GCCCGTCTCC 
TTCAGCAAGG TCAAGCTCAC CAACAAGCTC AACGGCGGCG GGCAGATCAT GCTCAACTCC 
CTGCACAAGT ACGAGCCCCG CATCCACATC GTGCGGGTGG GCGGCCCGCA GCGGATGATC 
ACCAGCCATT CCTTCCCCGA GACCCAGTTC ATCGCTGTGA CGGCGTACCA GAACGAGGAG 
ATCACAGCTT TAAAAATTAA ATACAACCCA TTTGCGAAGG CATTTCTTGA CGCAAAGGAA 
AGAAATGATC ACAAAGACAT GATGGAGGAA GCGGGAGACA ACCAGCAGTC TGGATACTCG 
CAGTTAGGTA GTTGGCTTAT TCCCGGGACT GGGGCTCTGT GCCCACCTGC CAATCCTCAC 
TCTCAGTTTG GAGCACCCCT GTCTCTCTCC CCTGCTCACA GCTGTGAAAG GTACTCACCG 
CTGAGGAATC ACCGTTCTGC CCCCTACCCC AATCCCTACA CCCATAGAAA CAACTCACCA 
ACAGCTTACA CTGATAACTC CTCTGCCTGC CTTCCCATGC TCCAGTCCCA TGACAATTGG 
TCCTCCCTGG GAGTTCCCAC ACACACGACT ATGCTGCCCA TGAGCCACAG CACTGGCACG 
GCTACCAGCT CCAGTCAGTA CCCGAACTTA TGGTCCGTGA GTAACAGCAC CATCACCCCG 
GCGCCTCAGT CAAGCGGGAT GTCCAACGGC CTGAGCTCCC AGTTCCTGCG TGGCTCTCCG 
GTGCACTACA CCGCTCTCCC ACATCCCGTC ACTGCCACCA CCTCCACATC CCCACTGTAC 
GATGGAGGAG CACCTGCAGA CCTTCCTGAC AGCCAGTACG ATGCCTCTGC ACACACTCGG 
TTGGCATCCA TGTGGACGCC CATCACCCCA CCTTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

T ( XM_015284045.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139531.2 Gallus gallus T brachyury transcription factor (T), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa148575
Clone ID Related Accession (Same CDS sequence)	XM_015284045.2
Accession Version	XM_015284045.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	brachyury protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015284045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCC CGGAGGACGC GGGCAAGGCG CCGGCGTACC GAGTGGACCA CCTGCTGAGC 
GCGGTGGAGA GCGAGCTGCA GGCGGGCAGC GAGAAGGGAG ACCCGACGGA GCGCGAGCTG 
CGCGTGGCGC TGGAGGACGG CGAGCTGTGG CTGCGCTTCA AGGAGCTCAC CAACGAGATG 
ATCGTCACCA AGAACGGCAG GAGGATGTTC CCGGTGCTGA AGGTGAGCGT GTCGGGGCTG 
GACCCCAACG CCATGTACTC CTTCCTGCTG GACTTCGTGG CGGCCGACGG GCACCGCTGG 
AAGTACGTCA ACGGCGAGTG GGTGCCGGGC GGCAAGCCGG AGCCGCAGGC GCCCAGCTGC 
GTCTACATCC ACCCCGACTC GCCCAACTTC GGCGCCCACT GGATGAAGGC GCCCGTCTCC 
TTCAGCAAGG TCAAGCTCAC CAACAAGCTC AACGGCGGCG GGCAGATCAT GCTCAACTCC 
CTGCACAAGT ACGAGCCCCG CATCCACATC GTGCGGGTGG GCGGCCCGCA GCGGATGATC 
ACCAGCCATT CCTTCCCCGA GACCCAGTTC ATCGCTGTGA CGGCGTACCA GAACGAGGAG 
ATCACAGCTT TAAAAATTAA ATACAACCCA TTTGCGAAGG CATTTCTTGA CGCAAAGGAA 
AGAAATGATC ACAAAGACAT GATGGAGGAA GCGGGAGACA ACCAGCAGTC TGGATACTCG 
CAGTTAGGTA GTTGGCTTAT TCCCGGGACT GGGGCTCTGT GCCCACCTGC CAATCCTCAC 
TCTCAGTTTG GAGCACCCCT GTCTCTCTCC CCTGCTCACA GCTGTGAAAG GTACTCACCG 
CTGAGGAATC ACCGTTCTGC CCCCTACCCC AATCCCTACA CCCATAGAAA CAACTCACCA 
ACTTACACTG ATAACTCCTC TGCCTGCCTT CCCATGCTCC AGTCCCATGA CAATTGGTCC 
TCCCTGGGAG TTCCCACACA CACGACTATG CTGCCCATGA GCCACAGCAC TGGCACGGCT 
ACCAGCTCCA GTCAGTACCC GAACTTATGG TCCGTGAGTA ACAGCACCAT CACCCCGGCG 
CCTCAGTCAA GCGGGATGTC CAACGGCCTG AGCTCCCAGT TCCTGCGTGG CTCTCCGGTG 
CACTACACCG CTCTCCCACA TCCCGTCACT GCCACCACCT CCACATCCCC ACTGTACGAT 
GGAGGAGCAC CTGCAGACCT TCCTGACAGC CAGTACGATG CCTCTGCACA CACTCGGTTG 
GCATCCATGT GGACGCCCAT CACCCCACCT TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139531.2
CDS	54..1352
Translation	MGSPEDAGKAPAYRVDHLLSAVESELQAGSEKGDPTERELRVALEDGELWLRFKELTNEMIVTKNGRRMFPVLKVSVSGLDPNAMYSFLLDFVAADGHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHSFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERNDHKDMMEEAGDNQQSGYSQLGSWLIPGTGALCPPANPHSQFGAPLSLSPAHSCERYSPLRNHRSAPYPNPYTHRNNSPTYTDNSSACLPMLQSHDNWSSLGVPTHTTMLPMSHSTGTATSSSQYPNLWSVSNSTITPAPQSSGMSNGLSSQFLRGSPVHYTALPHPVTATTSTSPLYDGGAPADLPDSQYDASAHTRLASMWTPITPPSM

Target ORF information:

RefSeq Version	XM_015284045.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus T brachyury transcription factor (T), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284045.2

ORF Insert Sequence:

ATGGGCTCCC CGGAGGACGC GGGCAAGGCG CCGGCGTACC GAGTGGACCA CCTGCTGAGC 
GCGGTGGAGA GCGAGCTGCA GGCGGGCAGC GAGAAGGGAG ACCCGACGGA GCGCGAGCTG 
CGCGTGGCGC TGGAGGACGG CGAGCTGTGG CTGCGCTTCA AGGAGCTCAC CAACGAGATG 
ATCGTCACCA AGAACGGCAG GAGGATGTTC CCGGTGCTGA AGGTGAGCGT GTCGGGGCTG 
GACCCCAACG CCATGTACTC CTTCCTGCTG GACTTCGTGG CGGCCGACGG GCACCGCTGG 
AAGTACGTCA ACGGCGAGTG GGTGCCGGGC GGCAAGCCGG AGCCGCAGGC GCCCAGCTGC 
GTCTACATCC ACCCCGACTC GCCCAACTTC GGCGCCCACT GGATGAAGGC GCCCGTCTCC 
TTCAGCAAGG TCAAGCTCAC CAACAAGCTC AACGGCGGCG GGCAGATCAT GCTCAACTCC 
CTGCACAAGT ACGAGCCCCG CATCCACATC GTGCGGGTGG GCGGCCCGCA GCGGATGATC 
ACCAGCCATT CCTTCCCCGA GACCCAGTTC ATCGCTGTGA CGGCGTACCA GAACGAGGAG 
ATCACAGCTT TAAAAATTAA ATACAACCCA TTTGCGAAGG CATTTCTTGA CGCAAAGGAA 
AGAAATGATC ACAAAGACAT GATGGAGGAA GCGGGAGACA ACCAGCAGTC TGGATACTCG 
CAGTTAGGTA GTTGGCTTAT TCCCGGGACT GGGGCTCTGT GCCCACCTGC CAATCCTCAC 
TCTCAGTTTG GAGCACCCCT GTCTCTCTCC CCTGCTCACA GCTGTGAAAG GTACTCACCG 
CTGAGGAATC ACCGTTCTGC CCCCTACCCC AATCCCTACA CCCATAGAAA CAACTCACCA 
ACTTACACTG ATAACTCCTC TGCCTGCCTT CCCATGCTCC AGTCCCATGA CAATTGGTCC 
TCCCTGGGAG TTCCCACACA CACGACTATG CTGCCCATGA GCCACAGCAC TGGCACGGCT 
ACCAGCTCCA GTCAGTACCC GAACTTATGG TCCGTGAGTA ACAGCACCAT CACCCCGGCG 
CCTCAGTCAA GCGGGATGTC CAACGGCCTG AGCTCCCAGT TCCTGCGTGG CTCTCCGGTG 
CACTACACCG CTCTCCCACA TCCCGTCACT GCCACCACCT CCACATCCCC ACTGTACGAT 
GGAGGAGCAC CTGCAGACCT TCCTGACAGC CAGTACGATG CCTCTGCACA CACTCGGTTG 
GCATCCATGT GGACGCCCAT CACCCCACCT TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

T ( XM_015284045.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139531.1 Gallus gallus T, brachyury homolog (mouse) (T), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa33577
Clone ID Related Accession (Same CDS sequence)	XM_015284045.1
Accession Version	XM_015284045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	brachyury protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCC CGGAGGACGC GGGCAAGGCG CCGGCGTACC GAGTGGACCA CCTGCTGAGC 
GCGGTGGAGA GCGAGCTGCA GGCGGGCAGC GAGAAGGGAG ACCCGACGGA GCGCGAGCTG 
CGCGTGGCGC TGGAGGACGG CGAGCTGTGG CTGCGCTTCA AGGAGCTCAC CAACGAGATG 
ATCGTCACCA AGAACGGCAG GAGGATGTTC CCGGTGCTGA AGGTGAGCGT GTCGGGGCTG 
GACCCCAACG CCATGTACTC CTTCCTGCTG GACTTCGTGG CGGCCGACGG GCACCGCTGG 
AAGTACGTCA ATGGAGAATG GGTGCCAGCT GGGAAACCAG AGCCGCAGGC GCCCAGCTGT 
GTCTACATCC ACCCCGACTC GCCCAACTTC GGCGCCCACT GGATGAAGGC GCCCGTCTCC 
TTCAGCAAGG TCAAGCTCAC CAACAAGCTC AACGGCGGCG GGCAGATCAT GCTCAACTCC 
CTGCACAAGT ACGAGCCCCG CATCCACATC GTGCGGGTGG GCGGCCCGCA GCGGATGATC 
ACCAGCCATT CCTTCCCCGA GACCCAGTTC ATCGCTGTGA CGGCGTACCA GAACGAGGAG 
ATCACAGCTT TAAAAATTAA ATACAACCCA TTTGCGAAGG CATTTCTTGA CGCAAAGGAA 
AGAAATGATC ACAAAGACAT GATGGAGGAA GCGGGAGACA ACCAGCAGTC TGGATACTCG 
CAGTTAGGTA GTTGGCTTAT TCCCGGGACT GGGGCTCTGT GCCCACCTGC CAATCCTCAC 
TCTCAGTTTG GAGCACCCCT GTCTCTCTCC CCTGCTCACA GCTGTGAAAG GTACTCACCG 
CTGAGGAATC ACCGTTCTGC CCCCTACCCC AATCCCTACA CCCATAGAAA CAACTCACCA 
ACTTACACTG ATAACTCCTC TGCCTGCCTT CCCATGCTCC AGTCCCATGA CAATTGGTCC 
TCCCTGGGAG TTCCCACACA CACGACTATG CTGCCCATGA GCCACAGCAC TGGCACGGCT 
ACCAGCTCCA GTCAGTACCC GAACTTATGG TCCGTGAGTA ACAGCACCAT CACCCCGGCG 
CCTCAGTCAA GCGGGATGTC CAACGGCCTG AGCTCCCAGT TCCTGCGTGG CTCTCCGGTG 
CACTACACCG CTCTCCCACA TCCCGTCACT GCCACCACCT CCACATCCCC ACTGTACGAT 
GGAGGAGCAC CTGCAGACCT TCCTGACAGC CAGTACGATG CCTCTGCACA CACTCGGTTG 
GCATCCATGT GGACGCCCAT CACCCCACCT TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139531.1
CDS	19..1317
Translation	MGSPEDAGKAPAYRVDHLLSAVESELQAGSEKGDPTERELRVALEDGELWLRFKELTNEMIVTKNGRRMFPVLKVSVSGLDPNAMYSFLLDFVAADGHRWKYVNGEWVPAGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHSFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERNDHKDMMEEAGDNQQSGYSQLGSWLIPGTGALCPPANPHSQFGAPLSLSPAHSCERYSPLRNHRSAPYPNPYTHRNNSPTYTDNSSACLPMLQSHDNWSSLGVPTHTTMLPMSHSTGTATSSSQYPNLWSVSNSTITPAPQSSGMSNGLSSQFLRGSPVHYTALPHPVTATTSTSPLYDGGAPADLPDSQYDASAHTRLASMWTPITPPSM

Target ORF information:

RefSeq Version	XM_015284045.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus T, brachyury homolog (mouse) (T), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015284045.1

ORF Insert Sequence:

ATGGGCTCCC CGGAGGACGC GGGCAAGGCG CCGGCGTACC GAGTGGACCA CCTGCTGAGC 
GCGGTGGAGA GCGAGCTGCA GGCGGGCAGC GAGAAGGGAG ACCCGACGGA GCGCGAGCTG 
CGCGTGGCGC TGGAGGACGG CGAGCTGTGG CTGCGCTTCA AGGAGCTCAC CAACGAGATG 
ATCGTCACCA AGAACGGCAG GAGGATGTTC CCGGTGCTGA AGGTGAGCGT GTCGGGGCTG 
GACCCCAACG CCATGTACTC CTTCCTGCTG GACTTCGTGG CGGCCGACGG GCACCGCTGG 
AAGTACGTCA ATGGAGAATG GGTGCCAGCT GGGAAACCAG AGCCGCAGGC GCCCAGCTGT 
GTCTACATCC ACCCCGACTC GCCCAACTTC GGCGCCCACT GGATGAAGGC GCCCGTCTCC 
TTCAGCAAGG TCAAGCTCAC CAACAAGCTC AACGGCGGCG GGCAGATCAT GCTCAACTCC 
CTGCACAAGT ACGAGCCCCG CATCCACATC GTGCGGGTGG GCGGCCCGCA GCGGATGATC 
ACCAGCCATT CCTTCCCCGA GACCCAGTTC ATCGCTGTGA CGGCGTACCA GAACGAGGAG 
ATCACAGCTT TAAAAATTAA ATACAACCCA TTTGCGAAGG CATTTCTTGA CGCAAAGGAA 
AGAAATGATC ACAAAGACAT GATGGAGGAA GCGGGAGACA ACCAGCAGTC TGGATACTCG 
CAGTTAGGTA GTTGGCTTAT TCCCGGGACT GGGGCTCTGT GCCCACCTGC CAATCCTCAC 
TCTCAGTTTG GAGCACCCCT GTCTCTCTCC CCTGCTCACA GCTGTGAAAG GTACTCACCG 
CTGAGGAATC ACCGTTCTGC CCCCTACCCC AATCCCTACA CCCATAGAAA CAACTCACCA 
ACTTACACTG ATAACTCCTC TGCCTGCCTT CCCATGCTCC AGTCCCATGA CAATTGGTCC 
TCCCTGGGAG TTCCCACACA CACGACTATG CTGCCCATGA GCCACAGCAC TGGCACGGCT 
ACCAGCTCCA GTCAGTACCC GAACTTATGG TCCGTGAGTA ACAGCACCAT CACCCCGGCG 
CCTCAGTCAA GCGGGATGTC CAACGGCCTG AGCTCCCAGT TCCTGCGTGG CTCTCCGGTG 
CACTACACCG CTCTCCCACA TCCCGTCACT GCCACCACCT CCACATCCCC ACTGTACGAT 
GGAGGAGCAC CTGCAGACCT TCCTGACAGC CAGTACGATG CCTCTGCACA CACTCGGTTG 
GCATCCATGT GGACGCCCAT CACCCCACCT TCCATGTAA