HMGB1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMGB1
Entrez Gene ID	395724
Full Name	high mobility group box 1
Synonyms	HMG-1,HMG1
General protein information	Preferred Names high mobility group box 1 Names high mobility group protein B1 high mobility group 1 protein high mobility group protein 1 high mobility group protein HMG1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015277670.1	XP_015133156.1	high mobility group protein B1 isoform X1
NM_204902.2	NP_990233.1	high mobility group protein B1
XM_015277667.1	XP_015133153.1	high mobility group protein B1 isoform X1
XM_015277667.2	XP_015133153.1	high mobility group protein B1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HMGB1	NP_788785.1
Gallus gallus (chicken)	HMGB1	NP_990233.1
	HMGB1	XP_001100203.1
Canis lupus familiaris (dog)	HMGB1	NP_001002937.1
Rattus norvegicus (Norway rat)	LOC685520	XP_003749961.1
Pan troglodytes (chimpanzee)	HMGB1	XP_509611.1
Danio rerio (zebrafish)	hmgb1a	NP_955849.2
Homo sapiens (human)	HMGB1	NP_002119.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct domains in HMGB1 are involved in specific intramolecular and nucleosomal interactions.
Kawase T, Sato K, Ueda T, Yoshida M
Biochemistry47(52)13991-6(2008 Dec)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

The effect of the acidic tail on the DNA-binding properties of the HMG1,2 class of proteins: insights from tail switching and tail removal.
Lee KB, Thomas JO
Journal of molecular biology304(2)135-49(2000 Nov)

The chicken genome contains no HMG1 retropseudogenes but a functional HMG1 gene with long introns.
Lum HK, Lee KD, Yu G
Biochimica et biophysica acta1493(1-2)64-72(2000 Sep)

Selection of a cDNA clone for chicken high-mobility-group 1 (HMG1) protein through its unusually conserved 3'-untranslated region, and improved expression of recombinant HMG1 in Escherichia coli.
Lee KB, Brooks DJ, Thomas JO
Gene225(1-2)97-105(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

specific regions of the acidic tail participate in intramolecular interactions with Lys2 of HMGB1 and in intermolecular interactions with Lys36 and Lys37 of histone H3.
Title: Distinct domains in HMGB1 are involved in specific intramolecular and nucleosomal interactions.

The following HMGB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMGB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa26770	NM_204902.2 Latest version!	Gallus gallus high mobility group box 1 (HMGB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa30947	XM_015277670.1 Latest version!	Gallus gallus high mobility group box 1 (HMGB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OGa30947	XM_015277667.2 Latest version!	Gallus gallus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OGa30947	XM_015277667.1	Gallus gallus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMGB1 ( NM_204902.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990233.1 Gallus gallus high mobility group box 1 (HMGB1), mRNA.

Price & Availability↑

CloneID	OGa26770
Clone ID Related Accession (Same CDS sequence)	NM_204902.2
Accession Version	NM_204902.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	high mobility group protein B1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ851648.1. On Feb 27, 2005 this sequence version replaced NM_204902.1. ##Evidence-Data-START## Transcript exon combination :: AJ851648.1, Y17968.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAGGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990233.1
CDS	113..760
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSSKEKGKFEDMAKADKLRYEKEMKNYVPPKGETKKKFKDPNAPKRPPSAFFLFCSEFRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKVDAGKKVVAKAEKSKKKKEEEEDEDEDEEDEEDEEEEEEEEEDDDDDE

Target ORF information:

RefSeq Version	NM_204902.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus high mobility group box 1 (HMGB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204902.2

ORF Insert Sequence:

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAGGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGB1 ( XM_015277670.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133156.1 Gallus gallus high mobility group box 1 (HMGB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa30947
Clone ID Related Accession (Same CDS sequence)	XM_015277670.1 , XM_015277667.1 , XM_015277667.2
Accession Version	XM_015277670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	high mobility group protein B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAAGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133156.1
CDS	61..708
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSSKEKGKFEDMAKADKLRYEKEMKNYVPPKGETKKKFKDPNAPKRPPSAFFLFCSEFRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKVDAGKKVVAKAEKSKKKKEEEEDEDEDEEDEEDEEEEEEEEEDDDDDE

Target ORF information:

RefSeq Version	XM_015277670.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus high mobility group box 1 (HMGB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277670.1

ORF Insert Sequence:

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAAGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGB1 ( XM_015277667.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133153.1 Gallus gallus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa30947
Clone ID Related Accession (Same CDS sequence)	XM_015277670.1 , XM_015277667.1 , XM_015277667.2
Accession Version	XM_015277667.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	high mobility group protein B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455760.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAAGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133153.1
CDS	33..680
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSSKEKGKFEDMAKADKLRYEKEMKNYVPPKGETKKKFKDPNAPKRPPSAFFLFCSEFRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKVDAGKKVVAKAEKSKKKKEEEEDEDEDEEDEEDEEEEEEEEEDDDDDE

Target ORF information:

RefSeq Version	XM_015277667.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277667.1

ORF Insert Sequence:

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAAGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGB1 ( XM_015277667.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133153.1 Gallus gallus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa30947
Clone ID Related Accession (Same CDS sequence)	XM_015277670.1 , XM_015277667.1 , XM_015277667.2
Accession Version	XM_015277667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	high mobility group protein B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAAGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133153.1
CDS	50..697
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSSKEKGKFEDMAKADKLRYEKEMKNYVPPKGETKKKFKDPNAPKRPPSAFFLFCSEFRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKVDAGKKVVAKAEKSKKKKEEEEDEDEDEEDEEDEEEEEEEEEDDDDDE

Target ORF information:

RefSeq Version	XM_015277667.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277667.2

ORF Insert Sequence:

ATGGGCAAAG GTGATCCCAA GAAGCCGAGA GGTAAAATGT CTTCATACGC CTTCTTTGTG 
CAAACCTGCC GGGAGGAGCA CAAGAAGAAA CATCCAGATG CTTCAGTGAA CTTCTCAGAG 
TTCTCAAAAA AATGCTCAGA ACGATGGAAG ACTATGTCTT CTAAGGAGAA AGGGAAGTTT 
GAAGATATGG CAAAGGCTGA CAAGCTTCGT TATGAAAAAG AAATGAAAAA CTATGTACCA 
CCTAAGGGGG AAACAAAAAA GAAGTTCAAG GATCCAAATG CACCGAAGAG GCCTCCTTCG 
GCTTTTTTCT TGTTTTGCTC TGAGTTTCGT CCAAAAATCA AAGGAGAACA TCCCGGTCTG 
TCCATTGGGG ACGTGGCAAA GAAACTGGGA GAGATGTGGA ACAACACCGC AGCAGATGAT 
AAACAGCCTT ACGAAAAGAA GGCTGCTAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT 
GCATACCGGG CCAAAGGGAA GGTTGATGCA GGCAAAAAAG TAGTTGCCAA GGCCGAGAAG 
AGCAAGAAGA AGAAGGAAGA AGAGGAAGAT GAGGACGAAG ACGAAGAGGA TGAAGAGGAC 
GAAGAAGAGG AAGAAGAGGA GGAGGAAGAT GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct domains in HMGB1 are involved in specific intramolecular and nucleosomal interactions.
Biochemistry47(52)13991-6(2008 Dec)
Kawase T,Sato K,Ueda T,Yoshida M

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

The effect of the acidic tail on the DNA-binding properties of the HMG1,2 class of proteins: insights from tail switching and tail removal.
Journal of molecular biology304(2)135-49(2000 Nov)
Lee KB,Thomas JO

The chicken genome contains no HMG1 retropseudogenes but a functional HMG1 gene with long introns.
Biochimica et biophysica acta1493(1-2)64-72(2000 Sep)
Lum HK,Lee KD,Yu G

Selection of a cDNA clone for chicken high-mobility-group 1 (HMG1) protein through its unusually conserved 3'-untranslated region, and improved expression of recombinant HMG1 in Escherichia coli.
Gene225(1-2)97-105(1998 Dec)
Lee KB,Brooks DJ,Thomas JO