CHAT cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHAT
Entrez Gene ID	395314
Full Name	choline O-acetyltransferase
General protein information	Preferred Names choline O-acetyltransferase Names choline O-acetyltransferase CHOACTase choline acetylase choline acetyltransferase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015287956.1	XP_015143442.1	choline O-acetyltransferase isoform X1
XM_015287955.2	XP_015143441.1	choline O-acetyltransferase isoform X1
XM_015287955.1	XP_015143441.1	choline O-acetyltransferase isoform X1
XM_015287956.2	XP_015143442.1	choline O-acetyltransferase isoform X1
NM_204610.1	NP_989941.1	choline O-acetyltransferase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chat	NP_034021.1
Drosophila melanogaster (fruit fly)	Cha	NP_477004.5
Homo sapiens (human)	CHAT	NP_001136405.1
Pan troglodytes (chimpanzee)	CHAT	XP_003339420.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002370	XP_312586.5
Caenorhabditis elegans (roundworm)	cha-1	NP_001023603.1
Xenopus tropicalis (tropical clawed frog)	chat	XP_002933959.1
	CHAT	XP_002805679.1
Bos taurus (cattle)	CHAT	XP_002698999.2
Canis lupus familiaris (dog)	CHAT	XP_005637542.1
Rattus norvegicus (Norway rat)	Chat	NP_001164064.1
Gallus gallus (chicken)	CHAT	NP_989941.1
Danio rerio (zebrafish)	chat	NP_001124191.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of choline acetyltransferase as a mechanism for cholinergic dysfunction induced by amyloid-? peptide oligomers.
Nunes-Tavares N, Santos LE, Stutz B, Brito-Moreira J, Klein WL, Ferreira ST, de Mello FG
The Journal of biological chemistry287(23)19377-85(2012 Jun)

Cloning of chicken choline acetyltransferase and its expression in early embryonic retina.
Mukherjee RS, Hausman RE
Brain research. Molecular brain research129(1-2)54-66(2004 Oct)

Neuron-enriched cultures derived from spinal cord of 10-day-old chick embryos: influence of neuropeptides on neuronal survival, proliferation and cholinergic expression.
Kentroti S, Vernadakis A
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience10(6)535-44(1992 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Reduction in ChAT activity instigated by AbetaOs may thus be a relevant event in early stage AD pathology, preceding the loss of cholinergic neurons commonly observed in AD brains.
Title: Inhibition of choline acetyltransferase as a mechanism for cholinergic dysfunction induced by amyloid-? peptide oligomers.

cloning; a 5.7 kb mRNA corresponding to the ChAT message was detected in embryonic retina and post-hatch brain; analysis of ChAT mRNA in embryonic retina shows the message can be detected by E6 and its level increased during early retinal development
Title: Cloning of chicken choline acetyltransferase and its expression in early embryonic retina.

The following CHAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa26785	NM_204610.1 Latest version!	Gallus gallus choline O-acetyltransferase (CHAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa37260	XM_015287956.2 Latest version!	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa37260	XM_015287955.2 Latest version!	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OGa37260	XM_015287955.1	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa37260	XM_015287956.1	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHAT ( NM_204610.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989941.1 Gallus gallus choline O-acetyltransferase (CHAT), mRNA.

Price & Availability↑

CloneID	OGa26785
Clone ID Related Accession (Same CDS sequence)	NM_204610.1
Accession Version	NM_204610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Gallus gallus(chicken)
Product	choline O-acetyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY044155.1. ##Evidence-Data-START## Transcript exon combination :: AY044155.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
GAGACCACAA ACTGGGTGTT TAACTATTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAAAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGC TTTTTGTTTT GGATGTTGGC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AATGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CACCAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCGCAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989941.1
CDS	1..1923
Translation	MPDLEKDMQKKEKDSRSKDEPAVPKLPVPPLQQTLQMYLQCMKHLVPEEQFRKTKSIVEQFGVAGGLGESLQLILEERREETTNWVFNYWLDDMYLNNRLALPVNSSPAIIFARQNFKDVNDQLRFAANLISGVQDYKALLDSHALPVDFARGQLSGQPLCMKQYYGLFSSYRLPGHTKDTLVAQKSCVMPEPEHIIVACNNQLFVLDVGINFRRLSEGDLFTQLRKIAKMAENEEEMLPPIGLLTTDGRTEWAEARTILMKDSTNRDSLDMIERCICLVCLDGTSGVELNDTNMALQLLHGGGYHKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLLRADSVSELPAPRRLRWKCSPEIQAHLASSAEKLQRIVKNLDFIAYKFVNYGKEFIKKQKTSPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATAEAFAFVKAMIDDKPALSDSEKMQRFKDAIAAQTNYTILAITGMAIDNHLLGLREVAREHFKELPEIFTDETYLTSNRFILSTSQVPTPMEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSDMLAKAVEESLLEIRDLCNKCSSSTAKSLAKQEEATQLRSDRKL

Target ORF information:

RefSeq Version	NM_204610.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus choline O-acetyltransferase (CHAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204610.1

ORF Insert Sequence:

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
GAGACCACAA ACTGGGTGTT TAACTATTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAAAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGC TTTTTGTTTT GGATGTTGGC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AATGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CACCAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCGCAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( XM_015287956.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015143442.1 Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa37260
Clone ID Related Accession (Same CDS sequence)	XM_015287956.1 , XM_015287955.2 , XM_015287955.1 , XM_015287956.2
Accession Version	XM_015287956.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	choline O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015143442.1
CDS	1967..3889
Translation	MPDLEKDMQKKEKDSRSKDEPAVPKLPVPPLQQTLQMYLQCMKHLVPEEQFRKTKSIVEQFGVAGGLGESLQLILEERREKTTNWVFNYWLDDMYLNNRLALPVNSSPAIIFARQNFKDVNDQLRFAANLISGVQDYKALLDSHALPVDFARGQLSGQPLCMKQYYGLFSSYRLPGHTKDTLVAQKSCVMPEPEHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIAKMAENEEEMLPPIGLLTTDGRTEWAEARTILMKDSTNRDSLDMIERCICLVCLDGTSGVELNDTNMALQLLHGGGYHKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLLRADSVSELPAPRRLRWKCSPEIQAHLASSAEKLQRIVKNLDFIAYKFVNYGKEFIKKQKTSPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATAEAFAFVKAMIDDKPALSDSEKMQRFKDAIAAQTNYTILAITGMAIDNHLLGLREVAREHFKELPEIFTDETYLTSNRFILSTSQVPTTMEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSDMLAKAVEESLLEIRDLCNKCSSSTAKSLAKQEEATQLRSDHKL

Target ORF information:

RefSeq Version	XM_015287956.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287956.1

ORF Insert Sequence:

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( XM_015287955.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015143441.1 Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa37260
Clone ID Related Accession (Same CDS sequence)	XM_015287956.1 , XM_015287955.2 , XM_015287955.1 , XM_015287956.2
Accession Version	XM_015287955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	choline O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015143441.1
CDS	189..2111
Translation	MPDLEKDMQKKEKDSRSKDEPAVPKLPVPPLQQTLQMYLQCMKHLVPEEQFRKTKSIVEQFGVAGGLGESLQLILEERREKTTNWVFNYWLDDMYLNNRLALPVNSSPAIIFARQNFKDVNDQLRFAANLISGVQDYKALLDSHALPVDFARGQLSGQPLCMKQYYGLFSSYRLPGHTKDTLVAQKSCVMPEPEHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIAKMAENEEEMLPPIGLLTTDGRTEWAEARTILMKDSTNRDSLDMIERCICLVCLDGTSGVELNDTNMALQLLHGGGYHKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLLRADSVSELPAPRRLRWKCSPEIQAHLASSAEKLQRIVKNLDFIAYKFVNYGKEFIKKQKTSPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATAEAFAFVKAMIDDKPALSDSEKMQRFKDAIAAQTNYTILAITGMAIDNHLLGLREVAREHFKELPEIFTDETYLTSNRFILSTSQVPTTMEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSDMLAKAVEESLLEIRDLCNKCSSSTAKSLAKQEEATQLRSDHKL

Target ORF information:

RefSeq Version	XM_015287955.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287955.2

ORF Insert Sequence:

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( XM_015287955.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015143441.1 Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa37260
Clone ID Related Accession (Same CDS sequence)	XM_015287956.1 , XM_015287955.2 , XM_015287955.1 , XM_015287956.2
Accession Version	XM_015287955.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	choline O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015143441.1
CDS	187..2109
Translation	MPDLEKDMQKKEKDSRSKDEPAVPKLPVPPLQQTLQMYLQCMKHLVPEEQFRKTKSIVEQFGVAGGLGESLQLILEERREKTTNWVFNYWLDDMYLNNRLALPVNSSPAIIFARQNFKDVNDQLRFAANLISGVQDYKALLDSHALPVDFARGQLSGQPLCMKQYYGLFSSYRLPGHTKDTLVAQKSCVMPEPEHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIAKMAENEEEMLPPIGLLTTDGRTEWAEARTILMKDSTNRDSLDMIERCICLVCLDGTSGVELNDTNMALQLLHGGGYHKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLLRADSVSELPAPRRLRWKCSPEIQAHLASSAEKLQRIVKNLDFIAYKFVNYGKEFIKKQKTSPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATAEAFAFVKAMIDDKPALSDSEKMQRFKDAIAAQTNYTILAITGMAIDNHLLGLREVAREHFKELPEIFTDETYLTSNRFILSTSQVPTTMEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSDMLAKAVEESLLEIRDLCNKCSSSTAKSLAKQEEATQLRSDHKL

Target ORF information:

RefSeq Version	XM_015287955.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287955.1

ORF Insert Sequence:

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAT ( XM_015287956.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015143442.1 Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa37260
Clone ID Related Accession (Same CDS sequence)	XM_015287956.1 , XM_015287955.2 , XM_015287955.1 , XM_015287956.2
Accession Version	XM_015287956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	choline O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006093.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015143442.1
CDS	1579..3501
Translation	MPDLEKDMQKKEKDSRSKDEPAVPKLPVPPLQQTLQMYLQCMKHLVPEEQFRKTKSIVEQFGVAGGLGESLQLILEERREKTTNWVFNYWLDDMYLNNRLALPVNSSPAIIFARQNFKDVNDQLRFAANLISGVQDYKALLDSHALPVDFARGQLSGQPLCMKQYYGLFSSYRLPGHTKDTLVAQKSCVMPEPEHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIAKMAENEEEMLPPIGLLTTDGRTEWAEARTILMKDSTNRDSLDMIERCICLVCLDGTSGVELNDTNMALQLLHGGGYHKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLLRADSVSELPAPRRLRWKCSPEIQAHLASSAEKLQRIVKNLDFIAYKFVNYGKEFIKKQKTSPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATAEAFAFVKAMIDDKPALSDSEKMQRFKDAIAAQTNYTILAITGMAIDNHLLGLREVAREHFKELPEIFTDETYLTSNRFILSTSQVPTTMEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSDMLAKAVEESLLEIRDLCNKCSSSTAKSLAKQEEATQLRSDHKL

Target ORF information:

RefSeq Version	XM_015287956.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus choline O-acetyltransferase (CHAT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015287956.2

ORF Insert Sequence:

ATGCCTGATC TAGAAAAAGA CATGCAGAAG AAGGAGAAAG ATTCACGCAG TAAAGATGAG 
CCAGCAGTAC CAAAACTTCC AGTCCCACCA CTGCAACAGA CCTTACAGAT GTACCTTCAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGAGCAT CGTGGAACAG 
TTTGGAGTTG CAGGAGGCCT TGGGGAATCC TTGCAGCTAA TCCTCGAGGA AAGAAGGGAA 
AAGACCACAA ACTGGGTGTT TAACTACTGG CTGGATGACA TGTACCTCAA CAACCGTTTG 
GCTCTTCCAG TCAACTCCAG CCCAGCAATT ATCTTTGCTC GTCAGAATTT CAAGGATGTA 
AATGACCAGC TAAGGTTTGC TGCCAATCTT ATTTCCGGCG TGCAAGACTA TAAAGCTTTA 
CTAGACAGCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCAGCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAC 
ACGCTTGTGG CCCAGAAAAG CTGTGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ATGAAGAGGA AATGCTGCCT 
CCAATTGGCT TGCTGACGAC TGATGGAAGA ACAGAGTGGG CAGAGGCCAG GACAATCCTT 
ATGAAAGATT CTACTAATCG CGACTCTCTT GACATGATTG AACGATGTAT ATGCCTGGTG 
TGCCTGGACG GTACAAGTGG GGTTGAACTC AACGATACAA ACATGGCGTT GCAACTGCTA 
CATGGAGGAG GCTACCATAA AAATGGTGCC AATCGTTGGT ATGACAAACC CATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGCGGCACT GTGTGTGAAC ATTCCCCTTT CGACGGCATC 
GTACTGGTGC AGTGCACCGA ACACCTGCTC AAGCACATGA AAGAAAGCTC CAAGAAATTA 
CTCCGAGCCG ATTCAGTAAG CGAGCTTCCT GCTCCACGGA GATTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCACTT GGCATCCTCA GCAGAAAAAC TCCAGAGGAT AGTAAAAAAT 
CTGGACTTTA TTGCCTACAA GTTTGTGAAC TATGGAAAGG AATTTATTAA GAAACAGAAG 
ACCAGCCCAG ATGCCTACAT TCAAGTAGCA CTGCAGCTTG CATTTTACAG GTGCCACAGG 
AGACTTGTCC CTACCTATGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AACATTAGGT CTGCTACGGC GGAAGCGTTT GCTTTTGTGA AAGCAATGAT TGATGACAAG 
CCAGCTCTGT CGGATTCTGA GAAGATGCAG AGATTTAAGG ACGCAATTGC AGCTCAAACT 
AATTACACTA TTTTGGCTAT TACTGGAATG GCAATAGACA ATCACCTACT AGGGCTGAGA 
GAGGTGGCAC GAGAACACTT CAAGGAACTG CCGGAGATCT TTACAGATGA GACCTATTTG 
ACAAGCAATA GATTCATCCT CTCAACCAGC CAGGTTCCAA CAACAATGGA AATGTTCTGC 
TGCTATGGGC CCGTGGTGCC CAACGGTTAT GGGGCATGTT ATAATCCTCA GCCAGAACAC 
ATATTATTCT GCATTTCAAG CTTTAAAGAC TGTAAAGAAA CGTCTTCAGA CATGCTTGCC 
AAAGCCGTGG AAGAAAGTCT CCTAGAAATC AGAGACCTGT GCAACAAATG CAGCTCTTCT 
ACAGCAAAGT CACTTGCTAA ACAAGAGGAA GCCACACAGT TGCGGAGTGA CCACAAACTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of choline acetyltransferase as a mechanism for cholinergic dysfunction induced by amyloid-? peptide oligomers.
The Journal of biological chemistry287(23)19377-85(2012 Jun)
Nunes-Tavares N,Santos LE,Stutz B,Brito-Moreira J,Klein WL,Ferreira ST,de Mello FG

Cloning of chicken choline acetyltransferase and its expression in early embryonic retina.
Brain research. Molecular brain research129(1-2)54-66(2004 Oct)
Mukherjee RS,Hausman RE

Neuron-enriched cultures derived from spinal cord of 10-day-old chick embryos: influence of neuropeptides on neuronal survival, proliferation and cholinergic expression.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience10(6)535-44(1992 Dec)
Kentroti S,Vernadakis A

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CHAT cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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