NUF2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUF2
Entrez Gene ID	395135
Full Name	NUF2, NDC80 kinetochore complex component
Synonyms	CDCA1
General protein information	Preferred Names NUF2, NDC80 kinetochore complex component Names kinetochore protein Nuf2 NUF2, NDC80 kinetochore complex component, homolog cell division cycle associated 1 cell division cycle-associated protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204478.3	NP_989809.2	kinetochore protein Nuf2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nuf2	NP_001012028.1
Danio rerio (zebrafish)	nuf2	NP_956604.1
Homo sapiens (human)	NUF2	NP_113611.2
	NUF2	XP_001083340.1
Gallus gallus (chicken)	NUF2	NP_989809.2
Pan troglodytes (chimpanzee)	NUF2	XP_001174484.1
Mus musculus (house mouse)	Nuf2	NP_075773.2
Canis lupus familiaris (dog)	NUF2	XP_536145.2
Bos taurus (cattle)	NUF2	NP_001092481.1
Xenopus tropicalis (tropical clawed frog)	nuf2	NP_001120109.1

Gene Ontology
Bibliography

Related articles in PubMed

CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1.
R?gnier V, Vagnarelli P, Fukagawa T, Zerjal T, Burns E, Trouche D, Earnshaw W, Brown W
Molecular and cellular biology25(10)3967-81(2005 May)

The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis.
Mikami Y, Hori T, Kimura H, Fukagawa T
Molecular and cellular biology25(5)1958-70(2005 Mar)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells.
Hori T, Haraguchi T, Hiraoka Y, Kimura H, Fukagawa T
Journal of cell science116(Pt 16)3347-62(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NUF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00017	NM_204478.3 Latest version!	Gallus gallus NUF2, NDC80 kinetochore complex component (NUF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUF2 ( NM_204478.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989809.2 Gallus gallus NUF2, NDC80 kinetochore complex component (NUF2), mRNA.

Price & Availability↑

CloneID	OGa00017
Clone ID Related Accession (Same CDS sequence)	NM_204478.3
Accession Version	NM_204478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Gallus gallus(chicken)
Product	kinetochore protein Nuf2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ720907.1. On Aug 31, 2012 this sequence version replaced NM_204478.2. ##Evidence-Data-START## Transcript exon combination :: AJ720907.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGGCGC TCACCTTTCC CCGCTACAGC CCGGATGATA TCGTCGCTTA TCTCCGCAGC 
CACGTCCTGG TGGGAGCCGA AGCCCGCAAT CTCACCAAGG CCGACCTGTT CGCCACCCTT 
AAGCCTGAAG TTCTGCACAT GATTTTCATA AGGATTCTGC AGAAGGTGTA TGGAATCCGC 
CTGGAGCACT TCTACATGAT GCCGGTCAAC GTTGATATAG TGTATCCACA GATATTTGAA 
GGCTTTCTAC CTGTTTGTAA TCTGTATATT CATATGGAGC GCTTCCTTCC AGTGTGCCGG 
GTTAATGACT TTCAAATGTC TGATGTTATA AATCCAAAGG CAAAGAGGAC AGCTCGCTTC 
TTGAGTGGCA TCCTCAACTT TGTGCATTTC AGAGAGTGCC GGCGTGAGGC CTACTTGGAG 
TTGCAGCTGA ACTATAAATC TGCTATGGAG AAACACCAAC AGCTGGAGAC AGCAAATCAG 
GAGTTAGAAA TGAAGCTGGA GAAACTAAAC ACTGTTCCAG TTGAACAGCA AGCAGAGTTC 
AAGCAACTGT CAGATGATAT TCAAGAGTTG GAGCAACTTC TAAGCCATGA CTATCGTCGG 
AAAACAGCAG CATTGCAAGA AGTGATTTCA CAAAAGAAAT CAGACATTAC AGAGAGGACC 
CGAAAACTGA ATGAACTTAA AGTGACAATG GCTACTTTGA AAGAAGAACA AGAGCAGCTG 
AAATCAAAGA TTGTGGAGAG TCCAGAGGAA TTGAAGAATT ATAAGGAACT AATGAAAGAG 
ACTGTTAAGA AACTCAAGAA ATCCAAGCAA GAGGTTATTG AGAAATATGA AGGTTATCGA 
GACCTGGTTG AAGTTCTGCC ATCATGCCAA TTGGAGGTGC AGTTATATCA GAAGAAGATG 
GAAAGACAGG CAGCAAACGT GGAAAGACTG GCCAGTGTAT TATCAGAGGT CAGAAATCTG 
GAAGACCAGC TGGAGAGTGC TCAAATAGAG CTGAAAAAAG GGAAGACGGA TGAGATGTCC 
CTGAAGAGAC TGGTCACTGC GAAACATGAG AGGCTGTCTA CAGCTGAAAT AAGGATAAAA 
AAGAAGCGTG AAGATGTTGA GCAATATAAG CACACTGTAT TTGAATATTT TAACAGAGTT 
CAGGAAAAGA GGGGTGTTGT TTATGATAAA GTGACAGCGA TTCAGAAGGA AATCCAGCAG 
ATGAGGTTCA AGATCCAGCA ACTGAATGAG AATGCTGAAG AAGAAGAAAT GAAAGCTAAA 
GAGATATACC AAAACCTGAA GGCTGGATTG GAGAAGCGTC ACGATTCTCT CATAAAGACA 
GCAAAGAATT ATGCAGCCTC AAGAGAAGAT AAAATTGCTG AACTGCAGAA GGGACTGCTT 
AGAGTTCAGA GTCCTGGAAG TAGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989809.2
CDS	21..1430
Translation	MEALTFPRYSPDDIVAYLRSHVLVGAEARNLTKADLFATLKPEVLHMIFIRILQKVYGIRLEHFYMMPVNVDIVYPQIFEGFLPVCNLYIHMERFLPVCRVNDFQMSDVINPKAKRTARFLSGILNFVHFRECRREAYLELQLNYKSAMEKHQQLETANQELEMKLEKLNTVPVEQQAEFKQLSDDIQELEQLLSHDYRRKTAALQEVISQKKSDITERTRKLNELKVTMATLKEEQEQLKSKIVESPEELKNYKELMKETVKKLKKSKQEVIEKYEGYRDLVEVLPSCQLEVQLYQKKMERQAANVERLASVLSEVRNLEDQLESAQIELKKGKTDEMSLKRLVTAKHERLSTAEIRIKKKREDVEQYKHTVFEYFNRVQEKRGVVYDKVTAIQKEIQQMRFKIQQLNENAEEEEMKAKEIYQNLKAGLEKRHDSLIKTAKNYAASREDKIAELQKGLLRVQSPGSSS

Target ORF information:

RefSeq Version	NM_204478.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NUF2, NDC80 kinetochore complex component (NUF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204478.3

ORF Insert Sequence:

ATGGAGGCGC TCACCTTTCC CCGCTACAGC CCGGATGATA TCGTCGCTTA TCTCCGCAGC 
CACGTCCTGG TGGGAGCCGA AGCCCGCAAT CTCACCAAGG CCGACCTGTT CGCCACCCTT 
AAGCCTGAAG TTCTGCACAT GATTTTCATA AGGATTCTGC AGAAGGTGTA TGGAATCCGC 
CTGGAGCACT TCTACATGAT GCCGGTCAAC GTTGATATAG TGTATCCACA GATATTTGAA 
GGCTTTCTAC CTGTTTGTAA TCTGTATATT CATATGGAGC GCTTCCTTCC AGTGTGCCGG 
GTTAATGACT TTCAAATGTC TGATGTTATA AATCCAAAGG CAAAGAGGAC AGCTCGCTTC 
TTGAGTGGCA TCCTCAACTT TGTGCATTTC AGAGAGTGCC GGCGTGAGGC CTACTTGGAG 
TTGCAGCTGA ACTATAAATC TGCTATGGAG AAACACCAAC AGCTGGAGAC AGCAAATCAG 
GAGTTAGAAA TGAAGCTGGA GAAACTAAAC ACTGTTCCAG TTGAACAGCA AGCAGAGTTC 
AAGCAACTGT CAGATGATAT TCAAGAGTTG GAGCAACTTC TAAGCCATGA CTATCGTCGG 
AAAACAGCAG CATTGCAAGA AGTGATTTCA CAAAAGAAAT CAGACATTAC AGAGAGGACC 
CGAAAACTGA ATGAACTTAA AGTGACAATG GCTACTTTGA AAGAAGAACA AGAGCAGCTG 
AAATCAAAGA TTGTGGAGAG TCCAGAGGAA TTGAAGAATT ATAAGGAACT AATGAAAGAG 
ACTGTTAAGA AACTCAAGAA ATCCAAGCAA GAGGTTATTG AGAAATATGA AGGTTATCGA 
GACCTGGTTG AAGTTCTGCC ATCATGCCAA TTGGAGGTGC AGTTATATCA GAAGAAGATG 
GAAAGACAGG CAGCAAACGT GGAAAGACTG GCCAGTGTAT TATCAGAGGT CAGAAATCTG 
GAAGACCAGC TGGAGAGTGC TCAAATAGAG CTGAAAAAAG GGAAGACGGA TGAGATGTCC 
CTGAAGAGAC TGGTCACTGC GAAACATGAG AGGCTGTCTA CAGCTGAAAT AAGGATAAAA 
AAGAAGCGTG AAGATGTTGA GCAATATAAG CACACTGTAT TTGAATATTT TAACAGAGTT 
CAGGAAAAGA GGGGTGTTGT TTATGATAAA GTGACAGCGA TTCAGAAGGA AATCCAGCAG 
ATGAGGTTCA AGATCCAGCA ACTGAATGAG AATGCTGAAG AAGAAGAAAT GAAAGCTAAA 
GAGATATACC AAAACCTGAA GGCTGGATTG GAGAAGCGTC ACGATTCTCT CATAAAGACA 
GCAAAGAATT ATGCAGCCTC AAGAGAAGAT AAAATTGCTG AACTGCAGAA GGGACTGCTT 
AGAGTTCAGA GTCCTGGAAG TAGTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1.
Molecular and cellular biology25(10)3967-81(2005 May)
R?gnier V,Vagnarelli P,Fukagawa T,Zerjal T,Burns E,Trouche D,Earnshaw W,Brown W

The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis.
Molecular and cellular biology25(5)1958-70(2005 Mar)
Mikami Y,Hori T,Kimura H,Fukagawa T

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells.
Journal of cell science116(Pt 16)3347-62(2003 Aug)
Hori T,Haraguchi T,Hiraoka Y,Kimura H,Fukagawa T