LIX1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIX1
Entrez Gene ID	374264
Full Name	limb and CNS expressed 1
General protein information	Preferred Names limb and CNS expressed 1 Names protein limb expression 1 Lix1 homolog limb expression 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204347.1	NP_989678.1	protein limb expression 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	lix1	XP_686266.2
	LIX1	XP_001095680.1
Mus musculus (house mouse)	Lix1	NP_079957.2
Gallus gallus (chicken)	LIX1	NP_989678.1
Rattus norvegicus (Norway rat)	Lix1	NP_001099684.1
Pan troglodytes (chimpanzee)	LIX1	XP_526965.2
Bos taurus (cattle)	LIX1	NP_001071471.1
Homo sapiens (human)	LIX1	NP_694966.3
Canis lupus familiaris (dog)	LIX1	XP_546011.2
Xenopus tropicalis (tropical clawed frog)	lix1	XP_002933479.1

Gene Ontology
Bibliography

Related articles in PubMed

LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors.
McKey J, Martire D, de Santa Barbara P, Faure S
BMC biology1434(2016 Apr)

Cloning and expression analysis of chicken Lix1, a founding member of a novel gene family.
Swindell EC, Moeller C, Thaller C, Eichele G
Mechanisms of development109(2)405-8(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression of LIX1 must be tightly regulated to allow fine-tuning of the transcript levels and state of activation of the pro-proliferative transcriptional coactivator YAP1 to regulate proliferation rates of stomach mesenchymal progenitors and their differentiation
Title: LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors.

LIX1 is expressed during early chicken limb development in the nascent hindlimb bud. LIX1 is also expressed in the basal plate of rhombomeres 3 and 5, in pharyngeal and in foregut mesenchyme and in all facial primordia except for the mandibular arches.
Title: Cloning and expression analysis of chicken Lix1, a founding member of a novel gene family.

The following LIX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa26670	NM_204347.1 Latest version!	Gallus gallus limb and CNS expressed 1 (LIX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIX1 ( NM_204347.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989678.1 Gallus gallus limb and CNS expressed 1 (LIX1), mRNA.

Price & Availability↑

CloneID	OGa26670
Clone ID Related Accession (Same CDS sequence)	NM_204347.1
Accession Version	NM_204347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Gallus gallus(chicken)
Product	protein limb expression 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF351203.1. ##Evidence-Data-START## Transcript exon combination :: AF351203.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACAGAA CCTTGGAATC TTTGCAGCTG GTGATTGCCC GAGTTCTGCC TCACAGAGAC 
CCTGCCCTGG TGTTTAAGGA CTTGAATGTT GTAGCAATAT TGCAAGAGTT CTGGGAAAGC 
AAACAGAAGA AGAAGGCCGT ACTTCCAAGT GAAGGCATCA TTGTGTATGA GTCACTGAGC 
AGCCTGGGCC CACCATTTGT GAGTTATGTT ACCTTGCCTG GAGGAAGTTG TTTCGGTAAC 
TTTCAGAGCT GTTTAAGCAG AGCAGAGGCA AGGCGGGATG CTGCAAAAGT GGCTCTGATC 
AACTCTCTCT TTAATGAATT GCCCTGTCGC AGAATCACAA AGGAATTCAT CATGGAAAGT 
GTTCAGGAGG CTGTTTCCTC AACAAGTGGC AACCTAAAGG ATGCTGATGA CCCCAGCACT 
AGTATTGGAG CTTATCATTA CATGCTAGAA TCAAACATTG GAAAGACAAT GCTGGAATTT 
CAGGAGCTGA TGATTGTCTT TCAGCTTCTG CATTGGAATG GAAGCCTTAA AGCTCTACGG 
GAAACAAAGT GCTCCCGGCA GGAAGTAATT TCCTACTACT CCCAGTGTTC CTTAGATGAA 
AAGATGAGAA GTCACATGGC CCTGGACTGG ATTATGAAGG AGCAAGAATC TCCAGGAATT 
ATTTCCCAAG AGCTGCAAGT GGCACTGAGG GAACTGGAAG AAGTCAGGAA AGCTGGGCAT 
GAACTGAGGT TCTACAAAGA GAAGAAGGAA ATACTGAGCT TAGCACTGAG TCAGATCTAC 
AGTGATGAAG TTACCACTTC CTCATGGGAC AATCAAATGA GCTTGGCCCT ACATGGCTAC 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989678.1
CDS	1..846
Translation	MDRTLESLQLVIARVLPHRDPALVFKDLNVVAILQEFWESKQKKKAVLPSEGIIVYESLSSLGPPFVSYVTLPGGSCFGNFQSCLSRAEARRDAAKVALINSLFNELPCRRITKEFIMESVQEAVSSTSGNLKDADDPSTSIGAYHYMLESNIGKTMLEFQELMIVFQLLHWNGSLKALRETKCSRQEVISYYSQCSLDEKMRSHMALDWIMKEQESPGIISQELQVALRELEEVRKAGHELRFYKEKKEILSLALSQIYSDEVTTSSWDNQMSLALHGYR

Target ORF information:

RefSeq Version	NM_204347.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus limb and CNS expressed 1 (LIX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204347.1

ORF Insert Sequence:

ATGGACAGAA CCTTGGAATC TTTGCAGCTG GTGATTGCCC GAGTTCTGCC TCACAGAGAC 
CCTGCCCTGG TGTTTAAGGA CTTGAATGTT GTAGCAATAT TGCAAGAGTT CTGGGAAAGC 
AAACAGAAGA AGAAGGCCGT ACTTCCAAGT GAAGGCATCA TTGTGTATGA GTCACTGAGC 
AGCCTGGGCC CACCATTTGT GAGTTATGTT ACCTTGCCTG GAGGAAGTTG TTTCGGTAAC 
TTTCAGAGCT GTTTAAGCAG AGCAGAGGCA AGGCGGGATG CTGCAAAAGT GGCTCTGATC 
AACTCTCTCT TTAATGAATT GCCCTGTCGC AGAATCACAA AGGAATTCAT CATGGAAAGT 
GTTCAGGAGG CTGTTTCCTC AACAAGTGGC AACCTAAAGG ATGCTGATGA CCCCAGCACT 
AGTATTGGAG CTTATCATTA CATGCTAGAA TCAAACATTG GAAAGACAAT GCTGGAATTT 
CAGGAGCTGA TGATTGTCTT TCAGCTTCTG CATTGGAATG GAAGCCTTAA AGCTCTACGG 
GAAACAAAGT GCTCCCGGCA GGAAGTAATT TCCTACTACT CCCAGTGTTC CTTAGATGAA 
AAGATGAGAA GTCACATGGC CCTGGACTGG ATTATGAAGG AGCAAGAATC TCCAGGAATT 
ATTTCCCAAG AGCTGCAAGT GGCACTGAGG GAACTGGAAG AAGTCAGGAA AGCTGGGCAT 
GAACTGAGGT TCTACAAAGA GAAGAAGGAA ATACTGAGCT TAGCACTGAG TCAGATCTAC 
AGTGATGAAG TTACCACTTC CTCATGGGAC AATCAAATGA GCTTGGCCCT ACATGGCTAC 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors.
BMC biology1434(2016 Apr)
McKey J,Martire D,de Santa Barbara P,Faure S

Cloning and expression analysis of chicken Lix1, a founding member of a novel gene family.
Mechanisms of development109(2)405-8(2001 Dec)
Swindell EC,Moeller C,Thaller C,Eichele G