SCX cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCX
Entrez Gene ID	374101
Full Name	scleraxis bHLH transcription factor
Synonyms	SCXB
General protein information	Preferred Names scleraxis bHLH transcription factor Names basic helix-loop-helix transcription factor scleraxis class II bHLH protein scleraxis scleraxis homolog B
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204253.1	NP_989584.1	basic helix-loop-helix transcription factor scleraxis

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Scx	NP_942588.1
Gallus gallus (chicken)	SCXB	NP_989584.1
Homo sapiens (human)	SCXB	NP_001073983.1
Canis lupus familiaris (dog)	SCXA	XP_005628354.1
Rattus norvegicus (Norway rat)	Scx	NP_001123980.1
Xenopus tropicalis (tropical clawed frog)	LOC100490170	XP_004919846.1
Bos taurus (cattle)	LOC615157	XP_002692591.1
Danio rerio (zebrafish)	scxa	NP_001076538.1

Gene Ontology
Bibliography

Related articles in PubMed

Four and a half domain 2 (FHL2) scaffolding protein is a marker of connective tissues of developing digits and regulates fibrogenic differentiation of limb mesodermal progenitors.
Lorda-Diez CI, Montero JA, Sanchez-Fernandez C, Garcia-Porrero JA, Chimal-Monroy J, Hurle JM
Journal of tissue engineering and regenerative medicine(2018 Jan)

The tumor suppressor BTG1 is expressed in the developing digits and regulates skeletogenic differentiation of limb mesodermal progenitors in high density cultures.
Lorda-Diez CI, Montero JA, Garcia-Porrero JA, Hurle JM
Cell and tissue research364(2)299-308(2016 05)

Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
Lorda-Diez CI, Montero JA, Choe S, Garcia-Porrero JA, Hurle JM
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research29(3)735-48(2014 Mar)

Tgf?-Smad and MAPK signaling mediate scleraxis and proteoglycan expression in heart valves.
Barnette DN, Hulin A, Ahmed AS, Colige AC, Azhar M, Lincoln J
Journal of molecular and cellular cardiology65137-46(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of Btg1 induced intense upregulation of Scleraxis. In complementary knockdown experiments, this marker was regulated in an opposite fashion.
Title: The tumor suppressor BTG1 is expressed in the developing digits and regulates skeletogenic differentiation of limb mesodermal progenitors in high density cultures.

The stage dependent transcriptional regulation of Scleraxis by BMPs is a finding relevant to understand BMP's function in connective tissue differentiation.
Title: Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.

These studies identify an important role for Scx in regulating proteoglycans in embryonic and mature valve cells.
Title: Tgf?-Smad and MAPK signaling mediate scleraxis and proteoglycan expression in heart valves.

More mature neurogenic progenitor cells express Sox2 and the bHLH transcription factor, E2A, and not Delta1
Title: Progenitor cell maturation in the developing vertebrate retina.

TGF B treatments upregulate Scleraxis preceding both fibrogenesis in the micromass cultures and cartilage differentiation during interdigital ectopic chondrogenesis.
Title: Transforming growth factors beta coordinate cartilage and tendon differentiation in the developing limb mesenchyme.

The following SCX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00363	NM_204253.1 Latest version!	Gallus gallus scleraxis bHLH transcription factor (SCX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCX ( NM_204253.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989584.1 Gallus gallus scleraxis bHLH transcription factor (SCX), mRNA.

Price & Availability↑

CloneID	OGa00363
Clone ID Related Accession (Same CDS sequence)	NM_204253.1
Accession Version	NM_204253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-06
Organism	Gallus gallus(chicken)
Product	basic helix-loop-helix transcription factor scleraxis
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF505881.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF505881.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCTTCG CCATGCTGCG CCCCGCGGCC GGCCGTTACC TGTACCCCGA GATCAGCATG 
CTGTCGGAGG ACGAGGAGAA CGGCAGCGAG AGCTCGGGCT CCGACGAGAA GCCCTTCCAC 
CTGGACGCCG ACGGCTTCGG CATCAAAGCC GGCAAGAGGA GGAGCGGCAA GAAAGCCGGG 
CGGCTGCACC GCGAGCCCCG GCAGCGGCAC ACGGCCAACG CGCGAGAGCG CGACCGCACC 
AACAGCGTCA ACACCGCCTT CACGGCGCTG CGCACCCTCA TCCCCACCGA GCCGGCCGAT 
AGGAAGCTGT CCAAGATCGA GACGCTGCGC CTGGCCTCCA GCTACATCTC CCACCTGGGC 
AACGTGCTGC TGGTGGGAGA GGCGTGCGGC GACGGGCAGC CGTGCCACAC CAGCCCGGCT 
TTCTTCCACC ACGGCGGAGG AGGCGGCAGC CCCCCGCCAC GCGACTCCGA GAACTCCCAG 
CCCAAACAGA TCTGCACTTT CTGCCTCAGC AACCAGAGGA AGCTGAGTAA AGACCGCGAC 
AGGAAGACGG CGATCCGGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989584.1
CDS	180..743
Translation	MSFAMLRPAAGRYLYPEISMLSEDEENGSESSGSDEKPFHLDADGFGIKAGKRRSGKKAGRLHREPRQRHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVLLVGEACGDGQPCHTSPAFFHHGGGGGSPPPRDSENSQPKQICTFCLSNQRKLSKDRDRKTAIRS

Target ORF information:

RefSeq Version	NM_204253.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus scleraxis bHLH transcription factor (SCX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204253.1

ORF Insert Sequence:

ATGTCCTTCG CCATGCTGCG CCCCGCGGCC GGCCGTTACC TGTACCCCGA GATCAGCATG 
CTGTCGGAGG ACGAGGAGAA CGGCAGCGAG AGCTCGGGCT CCGACGAGAA GCCCTTCCAC 
CTGGACGCCG ACGGCTTCGG CATCAAAGCC GGCAAGAGGA GGAGCGGCAA GAAAGCCGGG 
CGGCTGCACC GCGAGCCCCG GCAGCGGCAC ACGGCCAACG CGCGAGAGCG CGACCGCACC 
AACAGCGTCA ACACCGCCTT CACGGCGCTG CGCACCCTCA TCCCCACCGA GCCGGCCGAT 
AGGAAGCTGT CCAAGATCGA GACGCTGCGC CTGGCCTCCA GCTACATCTC CCACCTGGGC 
AACGTGCTGC TGGTGGGAGA GGCGTGCGGC GACGGGCAGC CGTGCCACAC CAGCCCGGCT 
TTCTTCCACC ACGGCGGAGG AGGCGGCAGC CCCCCGCCAC GCGACTCCGA GAACTCCCAG 
CCCAAACAGA TCTGCACTTT CTGCCTCAGC AACCAGAGGA AGCTGAGTAA AGACCGCGAC 
AGGAAGACGG CGATCCGGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Four and a half domain 2 (FHL2) scaffolding protein is a marker of connective tissues of developing digits and regulates fibrogenic differentiation of limb mesodermal progenitors.
Journal of tissue engineering and regenerative medicine(2018 Jan)
Lorda-Diez CI,Montero JA,Sanchez-Fernandez C,Garcia-Porrero JA,Chimal-Monroy J,Hurle JM

The tumor suppressor BTG1 is expressed in the developing digits and regulates skeletogenic differentiation of limb mesodermal progenitors in high density cultures.
Cell and tissue research364(2)299-308(2016 05)
Lorda-Diez CI,Montero JA,Garcia-Porrero JA,Hurle JM

Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research29(3)735-48(2014 Mar)
Lorda-Diez CI,Montero JA,Choe S,Garcia-Porrero JA,Hurle JM

Tgf?-Smad and MAPK signaling mediate scleraxis and proteoglycan expression in heart valves.
Journal of molecular and cellular cardiology65137-46(2013 Dec)
Barnette DN,Hulin A,Ahmed AS,Colige AC,Azhar M,Lincoln J