LOC105937193 cDNA ORF clone, Fundulus heteroclitus(Mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105937193
Entrez Gene ID	105937193
Full Name	arylsulfatase I-like
Gene Type	protein-coding
Organism	Fundulus heteroclitus(Mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012878381.2	XP_012733835.2	arylsulfatase I-like
XM_012878381.1	XP_012733835.1	arylsulfatase I-like

Pathways from BioSystems
Gene Ontology

The following LOC105937193 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105937193 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu122319	XM_012878381.1 Latest version!	Fundulus heteroclitus arylsulfatase I-like (LOC105937193), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OFu132878	XM_012878381.2 Latest version!	Fundulus heteroclitus arylsulfatase I-like (LOC105937193), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105937193 ( XM_012878381.1 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_012733835.1 Fundulus heteroclitus arylsulfatase I-like (LOC105937193), mRNA.

Price & Availability↑

CloneID	OFu122319
Clone ID Related Accession (Same CDS sequence)	XM_012878381.1
Accession Version	XM_012878381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	arylsulfatase I-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGACT ATGATGGCAA AGTGGACTCA AAGAAGGCGG GAAAGCCCAG GCCACCGCAC 
CTTATCTTCA TCATGGTGGA CGACCAGGGT TACGGAGACA TCGGGTATCA CGGCTCAGAC 
ATCCACACTC CTGCGCTGGA CCAGCTCGCA GCAGAGGGCG TGAAACTGGA AAATTATTAC 
GTCCAGCCTA TTTGCTCACC CTCTCGCAGT CAGCTCATGA CCGGACGTTA CCAGATTCAC 
ACTGGTCTCC AACACTCGAT CATCCGGCCA CGACAGCCCC TCTGTTTGCC TCCAGACGTT 
CCCACTCTAC CTGAACGTCT GGCTGAGGCT GGATACGCCA CACACATGGT TGGGAAATGG 
CACCTGGGCT TCTCAAGGCC AAACTGCCTG CCCACGCAAC GTGGATTTCA GAGTTTCCTG 
GGAACCCTGA CGGGCAGCGG AGACCACTTC TCCTATCAGA GCTGCGACGG GGCAGAAGCG 
TGTGGATTTG ACTTGCACGA CGGGAGCAGG CCTGCCTGGG AGATGAGAGG GAACTACTCT 
ACGCTGCTTT ACATTCAGAG AGTTAAGAAG ATTTTGCAGA GCCACGACTC CCGTACACCG 
CTCTTCCTCT ACCTGTCCCT CCAGGCTGCA CACACTCCCC TGCAGGCACC AGACCTTTTT 
CTGCGACACT ATAACACACA GGGCAATCGT CAAAGGCGTC ACTACGCAGC TATGCTAAGC 
TGCTTAGACG AAGGGGTTGC ACAGCTTGTC AACGAGCTGA AGACCAGAGG GCTCTATCAA 
AACTCAGTCC TGATCTACTC ATCTGATAAC GGTGGACAGC CACTCTCAGG CGGGAGCAAC 
TGGCCACTGA GAGGAGGCAA GGGGACCTAC TGGGAGGGAG GCATCCGGGC TGTGGGGTTT 
GTCCACAGTC CCCTTTTGAA GAGGAAGGGG ATCGTAAGCA GAGCCCTGAT CCACGTTTCT 
GACTGGTATC CCACATTGCT CGGGCTTGCA GGGGCCCTGC GATCCCATCG CGACCTGGAC 
GGCCATAACG TCTGGGGTAG CATTGGCGAG GGCTTACCCT GCCCTCGCAC GGAAATCCTT 
TTCAACATCG ACCCCGTGTC CAGGAAACCC GGGGAGCCAT ATGACAAGGC GCTGGTACTC 
AATGGCTTCG GGATCTGGGA CACAGCTGTG AGAGCCGCCC TGAGGGTTGG AGATTGGAAG 
CTATTGACAG GAAATGTGGG CGATGGAGAC TGGATACCGC CACAGGCTCT TCCGGTTGGC 
CCAGAACGGT GGCAGAAACT CGAGAAGCGG CGCAACAAGC TGGGGAAGTC GGTGTGGCTG 
TTTAACATCA GCGCCGACCC ATATGAGAGG TTCGACCTCG CTGAGGTTCG ACCAAAGGTG 
GTGAAACATC TGCTGGTTAG GCTGGCAGAG TATAACCACA CAGCTGTGAT GGCCAGGAAC 
CCACCAGATG ATCCCATGGC TGACCCAGAG CTACACGGAG GTGTATGGAG CCCCTGGCTG 
GGCTTGGAGG GGCAAGAAGA AAATAAGAAA GATGACGACG CCAAAAAGGA GAAGATCCTA 
AAGGTTAAAC ATTGCAAATT ATGCAAATTA AGAGCCCTTT TCAAAAAGGT GGGATCACGT 
CTGGAAAGAA AAACACTTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012733835.1
CDS	1..1644
Translation	MCDYDGKVDSKKAGKPRPPHLIFIMVDDQGYGDIGYHGSDIHTPALDQLAAEGVKLENYYVQPICSPSRSQLMTGRYQIHTGLQHSIIRPRQPLCLPPDVPTLPERLAEAGYATHMVGKWHLGFSRPNCLPTQRGFQSFLGTLTGSGDHFSYQSCDGAEACGFDLHDGSRPAWEMRGNYSTLLYIQRVKKILQSHDSRTPLFLYLSLQAAHTPLQAPDLFLRHYNTQGNRQRRHYAAMLSCLDEGVAQLVNELKTRGLYQNSVLIYSSDNGGQPLSGGSNWPLRGGKGTYWEGGIRAVGFVHSPLLKRKGIVSRALIHVSDWYPTLLGLAGALRSHRDLDGHNVWGSIGEGLPCPRTEILFNIDPVSRKPGEPYDKALVLNGFGIWDTAVRAALRVGDWKLLTGNVGDGDWIPPQALPVGPERWQKLEKRRNKLGKSVWLFNISADPYERFDLAEVRPKVVKHLLVRLAEYNHTAVMARNPPDDPMADPELHGGVWSPWLGLEGQEENKKDDDAKKEKILKVKHCKLCKLRALFKKVGSRLERKTLF

Target ORF information:

RefSeq Version	XM_012878381.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus arylsulfatase I-like (LOC105937193), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012878381.1

ORF Insert Sequence:

ATGTGTGACT ATGATGGCAA AGTGGACTCA AAGAAGGCGG GAAAGCCCAG GCCACCGCAC 
CTTATCTTCA TCATGGTGGA CGACCAGGGT TACGGAGACA TCGGGTATCA CGGCTCAGAC 
ATCCACACTC CTGCGCTGGA CCAGCTCGCA GCAGAGGGCG TGAAACTGGA AAATTATTAC 
GTCCAGCCTA TTTGCTCACC CTCTCGCAGT CAGCTCATGA CCGGACGTTA CCAGATTCAC 
ACTGGTCTCC AACACTCGAT CATCCGGCCA CGACAGCCCC TCTGTTTGCC TCCAGACGTT 
CCCACTCTAC CTGAACGTCT GGCTGAGGCT GGATACGCCA CACACATGGT TGGGAAATGG 
CACCTGGGCT TCTCAAGGCC AAACTGCCTG CCCACGCAAC GTGGATTTCA GAGTTTCCTG 
GGAACCCTGA CGGGCAGCGG AGACCACTTC TCCTATCAGA GCTGCGACGG GGCAGAAGCG 
TGTGGATTTG ACTTGCACGA CGGGAGCAGG CCTGCCTGGG AGATGAGAGG GAACTACTCT 
ACGCTGCTTT ACATTCAGAG AGTTAAGAAG ATTTTGCAGA GCCACGACTC CCGTACACCG 
CTCTTCCTCT ACCTGTCCCT CCAGGCTGCA CACACTCCCC TGCAGGCACC AGACCTTTTT 
CTGCGACACT ATAACACACA GGGCAATCGT CAAAGGCGTC ACTACGCAGC TATGCTAAGC 
TGCTTAGACG AAGGGGTTGC ACAGCTTGTC AACGAGCTGA AGACCAGAGG GCTCTATCAA 
AACTCAGTCC TGATCTACTC ATCTGATAAC GGTGGACAGC CACTCTCAGG CGGGAGCAAC 
TGGCCACTGA GAGGAGGCAA GGGGACCTAC TGGGAGGGAG GCATCCGGGC TGTGGGGTTT 
GTCCACAGTC CCCTTTTGAA GAGGAAGGGG ATCGTAAGCA GAGCCCTGAT CCACGTTTCT 
GACTGGTATC CCACATTGCT CGGGCTTGCA GGGGCCCTGC GATCCCATCG CGACCTGGAC 
GGCCATAACG TCTGGGGTAG CATTGGCGAG GGCTTACCCT GCCCTCGCAC GGAAATCCTT 
TTCAACATCG ACCCCGTGTC CAGGAAACCC GGGGAGCCAT ATGACAAGGC GCTGGTACTC 
AATGGCTTCG GGATCTGGGA CACAGCTGTG AGAGCCGCCC TGAGGGTTGG AGATTGGAAG 
CTATTGACAG GAAATGTGGG CGATGGAGAC TGGATACCGC CACAGGCTCT TCCGGTTGGC 
CCAGAACGGT GGCAGAAACT CGAGAAGCGG CGCAACAAGC TGGGGAAGTC GGTGTGGCTG 
TTTAACATCA GCGCCGACCC ATATGAGAGG TTCGACCTCG CTGAGGTTCG ACCAAAGGTG 
GTGAAACATC TGCTGGTTAG GCTGGCAGAG TATAACCACA CAGCTGTGAT GGCCAGGAAC 
CCACCAGATG ATCCCATGGC TGACCCAGAG CTACACGGAG GTGTATGGAG CCCCTGGCTG 
GGCTTGGAGG GGCAAGAAGA AAATAAGAAA GATGACGACG CCAAAAAGGA GAAGATCCTA 
AAGGTTAAAC ATTGCAAATT ATGCAAATTA AGAGCCCTTT TCAAAAAGGT GGGATCACGT 
CTGGAAAGAA AAACACTTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105937193 ( XM_012878381.2 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_012733835.2 Fundulus heteroclitus arylsulfatase I-like (LOC105937193), mRNA.

Price & Availability↑

CloneID	OFu132878
Clone ID Related Accession (Same CDS sequence)	XM_012878381.2
Accession Version	XM_012878381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	arylsulfatase I-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234473.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012878381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGAT TATCTGGAGG CATGAGGAAA GCAGTCCACT CTGTGCTCTT CTTCGGGGCA 
AGTGTAATCT TGCTAGGTGG TCTTGGCTGC ATGTGTGACT ATGATGGCAA AGTGGACTCA 
AAGAAGGCGG GAAAGCCCAG GCCACCGCAC CTTATCTTCA TCATGGTGGA CGACCAGGGT 
TACGGAGACA TCGGGTATCA CGGCTCAGAC ATCCACACTC CTGCGCTGGA CCAGCTCGCA 
GCAGAGGGCG TGAAACTGGA AAATTATTAC GTCCAGCCTA TTTGCTCACC CTCTCGCAGT 
CAGCTCATGA CCGGACGTTA CCAGATTCAC ACTGGTCTCC AACACTCGAT CATCCGGCCA 
CGACAGCCCC TCTGTTTGCC TCCAGACGTT CCCACTCTAC CTGAACGTCT GGCTGAGGCT 
GGATACGCCA CACACATGGT TGGGAAATGG CACCTGGGCT TCTCAAGGCC AAACTGCCTG 
CCCACGCAAC GTGGATTTCA GAGTTTCCTG GGAACCCTGA CGGGCAGCGG AGACCACTTC 
TCCTATCAGA GCTGCGACGG GGCAGAAGCG TGTGGATTTG ACTTGCACGA CGGGAGCAGG 
CCTGCCTGGG AGATGAGAGG GAACTACTCT ACGCTGCTTT ACATTCAGAG AGTTAAGAAG 
ATTTTGCAGA GCCACGACTC CCGTACACCG CTCTTCCTCT ACCTGTCCCT CCAGGCTGCA 
CACACTCCCC TGCAGGCACC AGACCTTTTT CTGCGACACT ATAACACACA GGGCAATCGT 
CAAAGGCGTC ACTACGCAGC TATGCTAAGC TGCTTAGACG AAGGGGTTGC ACAGCTTGTC 
AACGAGCTGA AGACCAGAGG GCTCTATCAA AACTCAGTCC TGATCTACTC ATCTGATAAC 
GGTGGACAGC CACTCTCAGG CGGGAGCAAC TGGCCACTGA GAGGAGGCAA GGGGACCTAC 
TGGGAGGGAG GCATCCGGGC TGTGGGGTTT GTCCACAGTC CCCTTTTGAA GAGGAAGGGG 
ATCGTAAGCA GAGCCCTGAT CCACGTTTCT GACTGGTATC CCACATTGCT CGGGCTTGCA 
GGGGCCCTGC GATCCCATCG CGACCTGGAC GGCCATAACG TCTGGGGTAG CATTGGCGAG 
GGCTTACCCT GCCCTCGCAC GGAAATCCTT TTCAACATCG ACCCCGTGTC CAGGAAACCC 
GGGGAGCCAT ATGACAAGGC GCTGGTACTC AATGGCTTCG GGATCTGGGA CACAGCTGTG 
AGAGCCGCCC TGAGGGTTGG AGATTGGAAG CTATTGACAG GAAATGTGGG CGATGGAGAC 
TGGATACCGC CACAGGCTCT TCCGGTTGGC CCAGAACGGT GGCAGAAACT CGAGAAGCGG 
CGCAACAAGC TGGGGAAGTC GGTGTGGCTG TTTAACATCA GCGCCGACCC ATATGAGAGG 
TTCGACCTCG CTGAGGTTCG ACCAAAGGTG GTGAAACATC TGCTGGTTAG GCTGGCAGAG 
TATAACCACA CAGCTGTGAT GGCCAGGAAC CCACCAGATG ATCCCATGGC TGACCCAGAG 
CTACACGGAG GTGTATGGAG CCCCTGGCTG GGCTTGGAGG GGCAAGAAGA AAATAAGAAA 
GATGACGACG CCAAAAAGGA GAAGATCCTA AAGGTTAAAC ATTGCAAATT ATGCAAATTA 
AGAGCCCTTT TCAAAAAGGT GGGATCACGT CTGGAAAGAA AAACACTTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012733835.2
CDS	191..1924
Translation	MCRLSGGMRKAVHSVLFFGASVILLGGLGCMCDYDGKVDSKKAGKPRPPHLIFIMVDDQGYGDIGYHGSDIHTPALDQLAAEGVKLENYYVQPICSPSRSQLMTGRYQIHTGLQHSIIRPRQPLCLPPDVPTLPERLAEAGYATHMVGKWHLGFSRPNCLPTQRGFQSFLGTLTGSGDHFSYQSCDGAEACGFDLHDGSRPAWEMRGNYSTLLYIQRVKKILQSHDSRTPLFLYLSLQAAHTPLQAPDLFLRHYNTQGNRQRRHYAAMLSCLDEGVAQLVNELKTRGLYQNSVLIYSSDNGGQPLSGGSNWPLRGGKGTYWEGGIRAVGFVHSPLLKRKGIVSRALIHVSDWYPTLLGLAGALRSHRDLDGHNVWGSIGEGLPCPRTEILFNIDPVSRKPGEPYDKALVLNGFGIWDTAVRAALRVGDWKLLTGNVGDGDWIPPQALPVGPERWQKLEKRRNKLGKSVWLFNISADPYERFDLAEVRPKVVKHLLVRLAEYNHTAVMARNPPDDPMADPELHGGVWSPWLGLEGQEENKKDDDAKKEKILKVKHCKLCKLRALFKKVGSRLERKTLF

Target ORF information:

RefSeq Version	XM_012878381.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus arylsulfatase I-like (LOC105937193), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012878381.2

ORF Insert Sequence:

ATGTGCAGAT TATCTGGAGG CATGAGGAAA GCAGTCCACT CTGTGCTCTT CTTCGGGGCA 
AGTGTAATCT TGCTAGGTGG TCTTGGCTGC ATGTGTGACT ATGATGGCAA AGTGGACTCA 
AAGAAGGCGG GAAAGCCCAG GCCACCGCAC CTTATCTTCA TCATGGTGGA CGACCAGGGT 
TACGGAGACA TCGGGTATCA CGGCTCAGAC ATCCACACTC CTGCGCTGGA CCAGCTCGCA 
GCAGAGGGCG TGAAACTGGA AAATTATTAC GTCCAGCCTA TTTGCTCACC CTCTCGCAGT 
CAGCTCATGA CCGGACGTTA CCAGATTCAC ACTGGTCTCC AACACTCGAT CATCCGGCCA 
CGACAGCCCC TCTGTTTGCC TCCAGACGTT CCCACTCTAC CTGAACGTCT GGCTGAGGCT 
GGATACGCCA CACACATGGT TGGGAAATGG CACCTGGGCT TCTCAAGGCC AAACTGCCTG 
CCCACGCAAC GTGGATTTCA GAGTTTCCTG GGAACCCTGA CGGGCAGCGG AGACCACTTC 
TCCTATCAGA GCTGCGACGG GGCAGAAGCG TGTGGATTTG ACTTGCACGA CGGGAGCAGG 
CCTGCCTGGG AGATGAGAGG GAACTACTCT ACGCTGCTTT ACATTCAGAG AGTTAAGAAG 
ATTTTGCAGA GCCACGACTC CCGTACACCG CTCTTCCTCT ACCTGTCCCT CCAGGCTGCA 
CACACTCCCC TGCAGGCACC AGACCTTTTT CTGCGACACT ATAACACACA GGGCAATCGT 
CAAAGGCGTC ACTACGCAGC TATGCTAAGC TGCTTAGACG AAGGGGTTGC ACAGCTTGTC 
AACGAGCTGA AGACCAGAGG GCTCTATCAA AACTCAGTCC TGATCTACTC ATCTGATAAC 
GGTGGACAGC CACTCTCAGG CGGGAGCAAC TGGCCACTGA GAGGAGGCAA GGGGACCTAC 
TGGGAGGGAG GCATCCGGGC TGTGGGGTTT GTCCACAGTC CCCTTTTGAA GAGGAAGGGG 
ATCGTAAGCA GAGCCCTGAT CCACGTTTCT GACTGGTATC CCACATTGCT CGGGCTTGCA 
GGGGCCCTGC GATCCCATCG CGACCTGGAC GGCCATAACG TCTGGGGTAG CATTGGCGAG 
GGCTTACCCT GCCCTCGCAC GGAAATCCTT TTCAACATCG ACCCCGTGTC CAGGAAACCC 
GGGGAGCCAT ATGACAAGGC GCTGGTACTC AATGGCTTCG GGATCTGGGA CACAGCTGTG 
AGAGCCGCCC TGAGGGTTGG AGATTGGAAG CTATTGACAG GAAATGTGGG CGATGGAGAC 
TGGATACCGC CACAGGCTCT TCCGGTTGGC CCAGAACGGT GGCAGAAACT CGAGAAGCGG 
CGCAACAAGC TGGGGAAGTC GGTGTGGCTG TTTAACATCA GCGCCGACCC ATATGAGAGG 
TTCGACCTCG CTGAGGTTCG ACCAAAGGTG GTGAAACATC TGCTGGTTAG GCTGGCAGAG 
TATAACCACA CAGCTGTGAT GGCCAGGAAC CCACCAGATG ATCCCATGGC TGACCCAGAG 
CTACACGGAG GTGTATGGAG CCCCTGGCTG GGCTTGGAGG GGCAAGAAGA AAATAAGAAA 
GATGACGACG CCAAAAAGGA GAAGATCCTA AAGGTTAAAC ATTGCAAATT ATGCAAATTA 
AGAGCCCTTT TCAAAAAGGT GGGATCACGT CTGGAAAGAA AAACACTTTT CTAA