DIO3 cDNA ORF clone, Fundulus heteroclitus(Mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO3
Entrez Gene ID	105917207
Full Name	iodothyronine deiodinase 3
Gene Type	protein-coding
Organism	Fundulus heteroclitus(Mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012851946.2	XP_012707400.1	thyroxine 5-deiodinase
XM_012851946.1	XP_012707400.1	type III iodothyronine deiodinase

Pathways from BioSystems
Gene Ontology

The following DIO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu101666	XM_012851946.2 Latest version!	Fundulus heteroclitus iodothyronine deiodinase 3 (dio3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OFu101666	XM_012851946.1	Fundulus heteroclitus deiodinase, iodothyronine, type III (dio3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO3 ( XM_012851946.2 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_012707400.1 Fundulus heteroclitus iodothyronine deiodinase 3 (dio3), mRNA.

Price & Availability↑

CloneID	OFu101666
Clone ID Related Accession (Same CDS sequence)	XM_012851946.2 , XM_012851946.1
Accession Version	XM_012851946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	thyroxine 5-deiodinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012851946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA CTGTTAAAGC TGTAAAAAAC GCCGTAGTCT GCTTCGTCTT GCTGCCCCGT 
TTTCTCGTGG CAGCGGTTGT CTTCTGGCTG CTGGACTTTC TGTGCATCAG GAAAAGGTTT 
TTCTTCAGGA TGAAGGAGCA GGGAGGCGAT GCCATTGATC CCCCTCTGTG CATATCGGAC 
TCCAACCGCC TGTTCAGCCT TGAGTCCCTG AAGGCCGTCT GGCACGGTCA GAAGCTGGAC 
TTCCTGAAGG CGGCGCGCCT CGGACGCGGA GCGCCCAACA CCGAAGTTGT CCGGCTGCAG 
GACCAGCGGC GCTCCCGGAT CCTGGACTGC GCGCAGGGCG CCAGACCGCT CATCCTCAAC 
TTCGGCAGCT GCACCTGACC GCCGTTCATG GCGCGCCTGA AGGCTTTCCA GGAGGTCGCG 
CAGCAGAACG CGGACATAGC GGACACGGCG GTGGTGTACA TCGAGGAGGC GCATCCGTCC 
GACGGCTGGA CGAGCACCGA CGCGCCTTAC CAGATCCCGA AGCATCGGAG TCTGGAGGAG 
AGGCTGAGCG CGGCGCACCT GATCCACCTG GAGGTGCCCG GCTGCCTGGT GGTAGCGGAC 
AACATGGAGA ACTCCTCCAG CGCCGCGTAC GGGGCGTATT TCAACAGACT TTATATAGTG 
CAGGAGGGGA AGGTGGTGTA CCAAGGCGGC AGGGGACCTG AGGGGTACCG GATCTCAGAG 
CTCAGAGACT GGCTGGACCA GCACAGAAAA GAGCTGGAGA AAACAGACAA TAATCTAAAC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012707400.1
CDS	42..827
Translation	MMNTVKAVKNAVVCFVLLPRFLVAAVVFWLLDFLCIRKRFFFRMKEQGGDAIDPPLCISDSNRLFSLESLKAVWHGQKLDFLKAARLGRGAPNTEVVRLQDQRRSRILDCAQGARPLILNFGSCT*PPFMARLKAFQEVAQQNADIADTAVVYIEEAHPSDGWTSTDAPYQIPKHRSLEERLSAAHLIHLEVPGCLVVADNMENSSSAAYGAYFNRLYIVQEGKVVYQGGRGPEGYRISELRDWLDQHRKELEKTDNNLNV

Target ORF information:

RefSeq Version	XM_012851946.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus iodothyronine deiodinase 3 (dio3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012851946.2

ORF Insert Sequence:

ATGATGAATA CTGTTAAAGC TGTAAAAAAC GCCGTAGTCT GCTTCGTCTT GCTGCCCCGT 
TTTCTCGTGG CAGCGGTTGT CTTCTGGCTG CTGGACTTTC TGTGCATCAG GAAAAGGTTT 
TTCTTCAGGA TGAAGGAGCA GGGAGGCGAT GCCATTGATC CCCCTCTGTG CATATCGGAC 
TCCAACCGCC TGTTCAGCCT TGAGTCCCTG AAGGCCGTCT GGCACGGTCA GAAGCTGGAC 
TTCCTGAAGG CGGCGCGCCT CGGACGCGGA GCGCCCAACA CCGAAGTTGT CCGGCTGCAG 
GACCAGCGGC GCTCCCGGAT CCTGGACTGC GCGCAGGGCG CCAGACCGCT CATCCTCAAC 
TTCGGCAGCT GCACCTGACC GCCGTTCATG GCGCGCCTGA AGGCTTTCCA GGAGGTCGCG 
CAGCAGAACG CGGACATAGC GGACACGGCG GTGGTGTACA TCGAGGAGGC GCATCCGTCC 
GACGGCTGGA CGAGCACCGA CGCGCCTTAC CAGATCCCGA AGCATCGGAG TCTGGAGGAG 
AGGCTGAGCG CGGCGCACCT GATCCACCTG GAGGTGCCCG GCTGCCTGGT GGTAGCGGAC 
AACATGGAGA ACTCCTCCAG CGCCGCGTAC GGGGCGTATT TCAACAGACT TTATATAGTG 
CAGGAGGGGA AGGTGGTGTA CCAAGGCGGC AGGGGACCTG AGGGGTACCG GATCTCAGAG 
CTCAGAGACT GGCTGGACCA GCACAGAAAA GAGCTGGAGA AAACAGACAA TAATCTAAAC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIO3 ( XM_012851946.1 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_012707400.1 Fundulus heteroclitus deiodinase, iodothyronine, type III (dio3), mRNA.

Price & Availability↑

CloneID	OFu101666
Clone ID Related Accession (Same CDS sequence)	XM_012851946.2 , XM_012851946.1
Accession Version	XM_012851946.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	type III iodothyronine deiodinase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAATA CTGTTAAAGC TGTAAAAAAC GCCGTAGTCT GCTTCGTCTT GCTGCCCCGT 
TTTCTCGTGG CAGCGGTTGT CTTCTGGCTG CTGGACTTTC TGTGCATCAG GAAAAGGTTT 
TTCTTCAGGA TGAAGGAGCA GGGAGGCGAT GCCATTGATC CCCCTCTGTG CATATCGGAC 
TCCAACCGCC TGTTCAGCCT TGAGTCCCTG AAGGCCGTCT GGCACGGTCA GAAGCTGGAC 
TTCCTGAAGG CGGCGCGCCT CGGACGCGGA GCGCCCAACA CCGAAGTTGT CCGGCTGCAG 
GACCAGCGGC GCTCCCGGAT CCTGGACTGC GCGCAGGGCG CCAGACCGCT CATCCTCAAC 
TTCGGCAGCT GCACCTGACC GCCGTTCATG GCGCGCCTGA AGGCTTTCCA GGAGGTCGCG 
CAGCAGAACG CGGACATAGC GGACACGGCG GTGGTGTACA TCGAGGAGGC GCATCCGTCC 
GACGGCTGGA CGAGCACCGA CGCGCCTTAC CAGATCCCGA AGCATCGGAG TCTGGAGGAG 
AGGCTGAGCG CGGCGCACCT GATCCACCTG GAGGTGCCCG GCTGCCTGGT GGTAGCGGAC 
AACATGGAGA ACTCCTCCAG CGCCGCGTAC GGGGCGTATT TCAACAGACT TTATATAGTG 
CAGGAGGGGA AGGTGGTGTA CCAAGGCGGC AGGGGACCTG AGGGGTACCG GATCTCAGAG 
CTCAGAGACT GGCTGGACCA GCACAGAAAA GAGCTGGAGA AAACAGACAA TAATCTAAAC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012707400.1
CDS	31..816
Translation	MMNTVKAVKNAVVCFVLLPRFLVAAVVFWLLDFLCIRKRFFFRMKEQGGDAIDPPLCISDSNRLFSLESLKAVWHGQKLDFLKAARLGRGAPNTEVVRLQDQRRSRILDCAQGARPLILNFGSCT*PPFMARLKAFQEVAQQNADIADTAVVYIEEAHPSDGWTSTDAPYQIPKHRSLEERLSAAHLIHLEVPGCLVVADNMENSSSAAYGAYFNRLYIVQEGKVVYQGGRGPEGYRISELRDWLDQHRKELEKTDNNLNV

Target ORF information:

RefSeq Version	XM_012851946.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus deiodinase, iodothyronine, type III (dio3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012851946.1

ORF Insert Sequence:

ATGATGAATA CTGTTAAAGC TGTAAAAAAC GCCGTAGTCT GCTTCGTCTT GCTGCCCCGT 
TTTCTCGTGG CAGCGGTTGT CTTCTGGCTG CTGGACTTTC TGTGCATCAG GAAAAGGTTT 
TTCTTCAGGA TGAAGGAGCA GGGAGGCGAT GCCATTGATC CCCCTCTGTG CATATCGGAC 
TCCAACCGCC TGTTCAGCCT TGAGTCCCTG AAGGCCGTCT GGCACGGTCA GAAGCTGGAC 
TTCCTGAAGG CGGCGCGCCT CGGACGCGGA GCGCCCAACA CCGAAGTTGT CCGGCTGCAG 
GACCAGCGGC GCTCCCGGAT CCTGGACTGC GCGCAGGGCG CCAGACCGCT CATCCTCAAC 
TTCGGCAGCT GCACCTGACC GCCGTTCATG GCGCGCCTGA AGGCTTTCCA GGAGGTCGCG 
CAGCAGAACG CGGACATAGC GGACACGGCG GTGGTGTACA TCGAGGAGGC GCATCCGTCC 
GACGGCTGGA CGAGCACCGA CGCGCCTTAC CAGATCCCGA AGCATCGGAG TCTGGAGGAG 
AGGCTGAGCG CGGCGCACCT GATCCACCTG GAGGTGCCCG GCTGCCTGGT GGTAGCGGAC 
AACATGGAGA ACTCCTCCAG CGCCGCGTAC GGGGCGTATT TCAACAGACT TTATATAGTG 
CAGGAGGGGA AGGTGGTGTA CCAAGGCGGC AGGGGACCTG AGGGGTACCG GATCTCAGAG 
CTCAGAGACT GGCTGGACCA GCACAGAAAA GAGCTGGAGA AAACAGACAA TAATCTAAAC 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.