LOC105916477 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105916477
Entrez Gene ID	105916477
Full Name	supervillin-like
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012851007.1	XP_012706461.1	supervillin-like
XM_021309733.1	XP_021165408.1	supervillin-like isoform X4
XM_021309730.1	XP_021165405.1	supervillin-like isoform X1
XM_021309734.1	XP_021165409.1	supervillin-like isoform X5
XM_021309731.1	XP_021165406.1	supervillin-like isoform X2
XM_021309732.1	XP_021165407.1	supervillin-like isoform X3
XM_021309735.1	XP_021165410.1	supervillin-like isoform X6
XM_021309729.1	XP_021165404.1	supervillin-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105916477 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105916477 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu100908	XM_012851007.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OFu128161	XM_021309735.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OFu128156	XM_021309730.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu128156	XM_021309729.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu128157	XM_021309731.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu128158	XM_021309732.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu128159	XM_021309733.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu128160	XM_021309734.1 Latest version!	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105916477 ( XM_012851007.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012706461.1 Fundulus heteroclitus supervillin-like (LOC105916477), mRNA.

Price & Availability↑

CloneID	OFu100908
Clone ID Related Accession (Same CDS sequence)	XM_012851007.1
Accession Version	XM_012851007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	supervillin-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGTG GTCCTGATAT TGGCCAAACC 
ACGTCTTCTT TGGACCTGGC TGCTGGTTCT GAGGGAAGCC GGCGGCGGAC CAGGCGTTAC 
CTTCCAGGCG CATCAGGAGG GGGTCGCAAA ACCAGCGAGC GCTTCAGGAC CCAGCCAATC 
ACAGCCAATG AGATGGAGGA GAGAACAGGG CTCTTGGACG CGGAAGTAAA TGACAACAAG 
AAAGCCGATG TGAAAACAGA TGATCGAGCC AAAATGAGCG TGGCAGCCAA GATGTCGCTG 
TTCAAAGAAT TGGAGAAGTC AGCGGCCCCC GATTCCTCGG CTCTGCTGAA GCCTCGCTCA 
GCCAGCAGCT TCCAGGAACG GAGGACGCGC CGTGGCAACG AGCATCGCTT TCTCACTCAG 
CCAATCACCT GTGAGGAAAT GGTGGCAATC AGCGCCCCTC CAGCAGAGGA GGTCCAGACC 
GAGCAGGCCG AACCGGTGGA GGCCGCAGAT GAAAGCTGCC GGATGAGCAT GAGAGAAAAG 
CTGGCCCTCT TCAACAGAGT GTCCCTGCAC GGAAAGAATG GGGCCGGCCC CACCGACGGG 
CCCCCAGAGA GGCGAAAGCA GAAAGGGGCT CGGTACCGCA CCCAGCCCAT CACAGTGGAC 
GAAGTCAGCC TGCTTCAGAA AGGCCCCATT CAGCTTCCTG CCTTCAGTTT GTCCCCTAAT 
CTGCACGACA GACAGCAGGC CTCGCCTGTC AACCTGAAAC CCAGCGAGGT GCGCCTTATA 
CAACCAATAT CTGAACTTTC AGAACCTGAA TCAACGCACC GGGGCTCGCG GGACTGCAAC 
TCCGTACCGC CGTCGAAGGG AATCCTGAAG AAGAGCTGCC CTGAAGGCTC AGAGTGGAGC 
GGGACCGGCG GTCGGGAGCC GCTACCCAAC CACCAACAAA ATGGTAGAGA CGTCACCGAT 
GCAGAGATGT TTGGGAGGAC GGAGGGAGCT GAGAGGCTGG AATCTCAGAG GGAGACACCG 
GGCGTGCAGC GAAGTTCAGT CCGAGCAGCT CCGTGGAGGC AGAGGGCTCG AAACCGGAGG 
GAGACCCCGA CACCGGCCTC CTCAGAGCGG GATGCTCCTC AGGAGGAGAG GCCTTCTCGG 
AGCGGCCCGC AGCAGGAGAT CGCTTCCTCT GTGGGAAACG GCTCAGATGT GACGAGGCAG 
GAGCCATCAG AAAGCCCTCC TCTTGAGACA CCTCAGTGCT GGGAACCCAT CTTTGCCTCT 
GTCTATTCTA TCAGTACACC CCAATATATC ATGTGTTTTA ACCAGACCAG CCTGTGCTTT 
GAGGCCCAAG AAGTCTCCTC TCCTACTAAG AGCGCAGTCC AGCCCCAGTG GAGGCAGAAG 
GCTAAAGAAG CCGAGGATGA GCTTCAGATG GAAGAAGCTG AAGAGAGGAC CGTGCAGCAG 
CAAAGCAGCG CACAGGAAGC TGCGATCAGC GTCAAGAGAA GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
TCCGTGCTTG CTGAACCACC CCAGCCTCAG TCGTCATCAT CGGACGGCCT GAGTCCCTGT 
GATGTTAAAG CTCTGGCGTC AGGCGGGCTG CTGGAGGAGG GCGTTTCCGT GCGGACCATT 
CTGGCTGGGG TCGACGTTCG TAGAGGCTGT GGGGTCATCA CCCTGGAGGA GGGCCATCAG 
ATGGAGCTGA GGACCGTCTC CGTGGACACC TGGCATGTCC AGGAGTTTGA CGACAGTGAA 
ATCCCAGTAG AGAGCACGGG GCAGCTGCAC GAAGGAGATT CCTATGTGAT CCGCTGGACA 
TACAGCATCA GTGCTGTTGA TAACCAGGAT GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA 
AACACAGCGT TTTTCTTGTG GCGTGGTCGG CACTCCAGCG TCAGCGGAGA AGACACAGCA 
GCTTTTCTAT CCATCGGGAT GAACGAGCAG GAAGACTCAC AGGTGGTCGT ACCTCAGGGT 
GCCGAACCCC CCTGCTTCCT GCAGCTCTTC CAGGGAGGCC TGGTCATCCA CAAGGGGAAG 
CGAGAGGAGG CCACCTCCAC TGCAGACTGG CGTCTGTTTT GCGTGCGTGG AGAGCTGCCA 
GAGGAGGCTT TGCTGCTAGA GGGGGAATGC TGCTGTTCTA GTCTGAGGTC CAGGGGCTCT 
GTAGTGCTGC TGAACAGCCA GCAGGGGGCG CTGTATCTCT GGACTGGCTG TAAAGCCCAT 
AGTGGCGCCC GTGAAGTCAG TAAGAGGGCA GTGGAGCGTC TCACTGAAAT GAGTCCATTG 
GAGCTGGGAC TCTGCAAAAA CAACCCTGTG AAGGTGCACT TTGTGGAGGA GGGTTCAGAA 
CCTGCAGACT TCTGGACGGC ACTGGGCCAG ATGAACAGGA AGGCCTATGA CTGCATGCTG 
CAAGATCCGG GAAAGTATAA CTTCACCCCC CGCCTCTTCC ACCTGAGTGC CTCTTCCGGT 
AAATTTAAAG CGGAGGAGCT CCAGAGTTTG ATCCGGCTGC CGGGGGTCGT GATGGCCATG 
CCCTTCGTCC AGGAAAGCTT GTACACTGTT CCTCAACCAG CCATGTTCCT GCTTGACAAC 
CGATTAGAGG TCTACCTGTG GCAGAGAGGT CAGCCTGAGC AAACGGAGAG CTCCGCTTCA 
GCCTGGAGCC TGTGGCATAA CGAGAGAAAG TGTGCCATGC AGACGGCTCT GCAGTACTGC 
AAAGAGATAA ACCCGAGACG GCCCCCGCAG GCGTATCTCA TCTTAGAGGG ATCAGAGCCT 
CTCACCTTCA CTAATGTCTT CCCCCGCTGG GAGAGGAACC TCGGACCCAC CACACAGTCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012706461.1
CDS	117..4559
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRQEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRRSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQS

Target ORF information:

RefSeq Version	XM_012851007.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012851007.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGTG GTCCTGATAT TGGCCAAACC 
ACGTCTTCTT TGGACCTGGC TGCTGGTTCT GAGGGAAGCC GGCGGCGGAC CAGGCGTTAC 
CTTCCAGGCG CATCAGGAGG GGGTCGCAAA ACCAGCGAGC GCTTCAGGAC CCAGCCAATC 
ACAGCCAATG AGATGGAGGA GAGAACAGGG CTCTTGGACG CGGAAGTAAA TGACAACAAG 
AAAGCCGATG TGAAAACAGA TGATCGAGCC AAAATGAGCG TGGCAGCCAA GATGTCGCTG 
TTCAAAGAAT TGGAGAAGTC AGCGGCCCCC GATTCCTCGG CTCTGCTGAA GCCTCGCTCA 
GCCAGCAGCT TCCAGGAACG GAGGACGCGC CGTGGCAACG AGCATCGCTT TCTCACTCAG 
CCAATCACCT GTGAGGAAAT GGTGGCAATC AGCGCCCCTC CAGCAGAGGA GGTCCAGACC 
GAGCAGGCCG AACCGGTGGA GGCCGCAGAT GAAAGCTGCC GGATGAGCAT GAGAGAAAAG 
CTGGCCCTCT TCAACAGAGT GTCCCTGCAC GGAAAGAATG GGGCCGGCCC CACCGACGGG 
CCCCCAGAGA GGCGAAAGCA GAAAGGGGCT CGGTACCGCA CCCAGCCCAT CACAGTGGAC 
GAAGTCAGCC TGCTTCAGAA AGGCCCCATT CAGCTTCCTG CCTTCAGTTT GTCCCCTAAT 
CTGCACGACA GACAGCAGGC CTCGCCTGTC AACCTGAAAC CCAGCGAGGT GCGCCTTATA 
CAACCAATAT CTGAACTTTC AGAACCTGAA TCAACGCACC GGGGCTCGCG GGACTGCAAC 
TCCGTACCGC CGTCGAAGGG AATCCTGAAG AAGAGCTGCC CTGAAGGCTC AGAGTGGAGC 
GGGACCGGCG GTCGGGAGCC GCTACCCAAC CACCAACAAA ATGGTAGAGA CGTCACCGAT 
GCAGAGATGT TTGGGAGGAC GGAGGGAGCT GAGAGGCTGG AATCTCAGAG GGAGACACCG 
GGCGTGCAGC GAAGTTCAGT CCGAGCAGCT CCGTGGAGGC AGAGGGCTCG AAACCGGAGG 
GAGACCCCGA CACCGGCCTC CTCAGAGCGG GATGCTCCTC AGGAGGAGAG GCCTTCTCGG 
AGCGGCCCGC AGCAGGAGAT CGCTTCCTCT GTGGGAAACG GCTCAGATGT GACGAGGCAG 
GAGCCATCAG AAAGCCCTCC TCTTGAGACA CCTCAGTGCT GGGAACCCAT CTTTGCCTCT 
GTCTATTCTA TCAGTACACC CCAATATATC ATGTGTTTTA ACCAGACCAG CCTGTGCTTT 
GAGGCCCAAG AAGTCTCCTC TCCTACTAAG AGCGCAGTCC AGCCCCAGTG GAGGCAGAAG 
GCTAAAGAAG CCGAGGATGA GCTTCAGATG GAAGAAGCTG AAGAGAGGAC CGTGCAGCAG 
CAAAGCAGCG CACAGGAAGC TGCGATCAGC GTCAAGAGAA GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
TCCGTGCTTG CTGAACCACC CCAGCCTCAG TCGTCATCAT CGGACGGCCT GAGTCCCTGT 
GATGTTAAAG CTCTGGCGTC AGGCGGGCTG CTGGAGGAGG GCGTTTCCGT GCGGACCATT 
CTGGCTGGGG TCGACGTTCG TAGAGGCTGT GGGGTCATCA CCCTGGAGGA GGGCCATCAG 
ATGGAGCTGA GGACCGTCTC CGTGGACACC TGGCATGTCC AGGAGTTTGA CGACAGTGAA 
ATCCCAGTAG AGAGCACGGG GCAGCTGCAC GAAGGAGATT CCTATGTGAT CCGCTGGACA 
TACAGCATCA GTGCTGTTGA TAACCAGGAT GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA 
AACACAGCGT TTTTCTTGTG GCGTGGTCGG CACTCCAGCG TCAGCGGAGA AGACACAGCA 
GCTTTTCTAT CCATCGGGAT GAACGAGCAG GAAGACTCAC AGGTGGTCGT ACCTCAGGGT 
GCCGAACCCC CCTGCTTCCT GCAGCTCTTC CAGGGAGGCC TGGTCATCCA CAAGGGGAAG 
CGAGAGGAGG CCACCTCCAC TGCAGACTGG CGTCTGTTTT GCGTGCGTGG AGAGCTGCCA 
GAGGAGGCTT TGCTGCTAGA GGGGGAATGC TGCTGTTCTA GTCTGAGGTC CAGGGGCTCT 
GTAGTGCTGC TGAACAGCCA GCAGGGGGCG CTGTATCTCT GGACTGGCTG TAAAGCCCAT 
AGTGGCGCCC GTGAAGTCAG TAAGAGGGCA GTGGAGCGTC TCACTGAAAT GAGTCCATTG 
GAGCTGGGAC TCTGCAAAAA CAACCCTGTG AAGGTGCACT TTGTGGAGGA GGGTTCAGAA 
CCTGCAGACT TCTGGACGGC ACTGGGCCAG ATGAACAGGA AGGCCTATGA CTGCATGCTG 
CAAGATCCGG GAAAGTATAA CTTCACCCCC CGCCTCTTCC ACCTGAGTGC CTCTTCCGGT 
AAATTTAAAG CGGAGGAGCT CCAGAGTTTG ATCCGGCTGC CGGGGGTCGT GATGGCCATG 
CCCTTCGTCC AGGAAAGCTT GTACACTGTT CCTCAACCAG CCATGTTCCT GCTTGACAAC 
CGATTAGAGG TCTACCTGTG GCAGAGAGGT CAGCCTGAGC AAACGGAGAG CTCCGCTTCA 
GCCTGGAGCC TGTGGCATAA CGAGAGAAAG TGTGCCATGC AGACGGCTCT GCAGTACTGC 
AAAGAGATAA ACCCGAGACG GCCCCCGCAG GCGTATCTCA TCTTAGAGGG ATCAGAGCCT 
CTCACCTTCA CTAATGTCTT CCCCCGCTGG GAGAGGAACC TCGGACCCAC CACACAGTCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309735.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165410.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OFu128161
Clone ID Related Accession (Same CDS sequence)	XM_021309735.1
Accession Version	XM_021309735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATGA GAGAAAAGCT GGCCCTCTTC AACAGAGTGT CCCTGCACGG AAAGAATGGG 
GCCGGCCCCA CCGACGGGCC CCCAGAGAGG CGAAAGCAGA AAGGGGCTCG GTACCGCACC 
CAGCCCATCA CAGTGGACGA AGTCAGCCTG CTTCAGAAAG GCCCCATTCA GCTTCCTGCC 
TTCAGTTTGT CCCCTAATCT GCACGACAGA CAGCAGGCCT CGCCTGTCAA CCTGAAACCC 
AGCGAGGTGC GCCTTATACA ACCAATATCT GAACTTTCAG AACCTGAATC AACGCACCGG 
GGCTCGCGGG ACTGCAACTC CGTACCGCCG TCGAAGGGAA TCCTGAAGAA GAGCTGCCCT 
GAAGGCTCAG AGTGGAGCGG GACCGGCGGT CGGGAGCCGC TACCCAACCA CCAACAAAAT 
GGTAGAGACG TCACCGATGC AGAGATGTTT GGGAGGACGG AGGGAGCTGA GAGGCTGGAA 
TCTCAGAGGG AGACACCGGG CGTGCAGCGA AGTTCAGTCC GAGCAGCTCC GTGGAGGCAG 
AGGGCTCGAA ACCGGAGGGA GACCCCGACA CCGGCCTCCT CAGAGCGGGA TGCTCCTCAG 
GAGGAGAGGC CTTCTCGGAG CGGCCCGCAG CAGGAGATCG CTTCCTCTGT GGGAAACGGC 
TCAGATGTGA CGAGAACACA AGATGAAGAG CAGAGCGACA AACCGAAACA AAACGTTTTG 
GTCTGCGGTG GAGTTCGCTC ACCTAAACAG CAGGAAGCAG CAGCATCTGT GAGAGGCACG 
GAGCCATCAG AAAGCCCTCC TCTTGAGACA CCTCAGTGCT GGGAACCCAT CTTTGCCTCT 
GTCTATTCTA TCAGTACACC CCAATATATC ATGTGTTTTA ACCAGACCAG CCTGTGCTTT 
GAGGCCCAAG AAGTCTCCTC TCCTACTAAG AGCGCAGTCC AGCCCCAGTG GAGGCAGAAG 
GCTAAAGAAG CCGAGGATGA GCTTCAGATG GAAGAAGCTG AAGAGAGGAC CGTGCAGCAG 
CAAAGCAGCG CACAGGAAGC TGCGATCAGC GTCAAGAGAA GTCCGGGATC TCCGGCTGAA 
CCTGACAGTA GGGATGTTTC CAAGAAGAAG ATGGACTCCA GTGAAGCTAC GACAGAGAGT 
GTCTCACACA TGAACCCGGC TCACGAGGAG GCACCAGTCG ATAGTTCCAA GATGTCGGAC 
GGAGGTTTCT GCGGCGGCTG CTTGCCTCCT CCAGCAGGCG GCCCCACACC GTGCGGCGAC 
GTGGCTGCGT CAGAGAGGGA GCGCGACTTT GCCACCCTCT GCCAAAGCAG CATGCCCATA 
CTCAGCTCAG CGGTCGCAGA GCACAGACGA GCAGTGCGTC CGTCTCGTCG GACCCAGGGC 
TCCAGAAACC CCCTTAGGGC TCTGGCAGCC AGAGAGGACA TCAGGCAGGA CTACATGGGG 
GACCGGGACA ACACCGCAGC CGAGGAGAGA GGCCAAGCAG AAAAAAACTG TAAGAACTCT 
TCCCCGCCGG AGTCACTTCC CATCACCTCT TCAGATAAGG AGACAACCAG GACTTTCCCT 
CCGTTCAGCA ACCTGATGCT AATTCACGTC AAGGGGAGGC GGCAGGTCCA GGTACGTCTG 
GTAGAACCCT CGGTCCGTTC GCTGAACAGC GGAGACTGCT TCCTGCTCGT CGCTCCGGAG 
CATTGCATCC TGTGGAGCGG AGAGTTTGCC AACAAAGAGG AGAGGGCAAA GGCCCTTGAA 
CTGGCAGCCT TTATCCAGGG CCATAGGGAC CTCGGCTGTC AGGCCCCCCA GGTGCTCCAC 
CTGGAGGAAG GCTTGAACTC CGATGGCGCC GACGCTGCAG ACTTTTGGAG CCTTCTCGGA 
GGGAGACAAC GTTACAGAGG AGCTGGTGCT CCAGAAGAGG ATGCGCTGTT TGAGCAAGGA 
GTGGTGGAGT CCAACTGTGT TTACAGCCTA GTGGAGGACA GATTGGTGCC TCACGAACGG 
GCCTGGGCCT CCGTCCCCAG CACCTCCCTG CTGGGCTCCT CTGAGGTCCT GGTGTTTGAT 
TTCGGCAGCG AGGTGTATCT GTGGCACGGA CGGGATGTTT CACCCGGCAG GAAGGACGTT 
GCCCTCCAGC TGACTCAGCA GGTGTGGGCG GGCGAGTACG ACTACGGCAA CTGTCGAGTC 
AACCCACTGG ATCCCACTCA GAGCAACCCC AGCGTTCAGC CGAGGGGAGA AGGCCGTCCC 
AGGTGGGCTC TGCTCGCCGT GGTCTCCGAG GACAGCGAGA CGGCTCTTTT CAGGGAAAAG 
TTCCAGGGCC CGACGCGTGG AAGCGGAGAC ACCGTAGAAG TGACTTCAGG GAGAGAAGAG 
ATGCAGGAAT ATTACAAACG ATTGATAATT TTCCAGTCCG TGCTTGCTGA ACCACCCCAG 
CCTCAGTCGT CATCATCGGA CGGCCTGAGT CCCTGTGATG TTAAAGCTCT GGCGTCAGGC 
GGGCTGCTGG AGGAGGGCGT TTCCGTGCGG ACCATTCTGG CTGGGGTCGA CGTTCGTAGA 
GGCTGTGGGG TCATCACCCT GGAGGAGGGC CATCAGATGG AGCTGAGGAC CGTCTCCGTG 
GACACCTGGC ATGTCCAGGA GTTTGACGAC AGTGAAATCC CAGTAGAGAG CACGGGGCAG 
CTGCACGAAG GAGATTCCTA TGTGATCCGC TGGACATACA GCATCAGTGC TGTTGATAAC 
CAGGATGAGT GTGGCAATGC TTCCAAATCT AAGGAAAACA CAGCGTTTTT CTTGTGGCGT 
GGTCGGCACT CCAGCGTCAG CGGAGAAGAC ACAGCAGCTT TTCTATCCAT CGGGATGAAC 
GAGCAGGAAG ACTCACAGGT GGTCGTACCT CAGGGTGCCG AACCCCCCTG CTTCCTGCAG 
CTCTTCCAGG GAGGCCTGGT CATCCACAAG GGGAAGCGAG AGGAGGCCAC CTCCACTGCA 
GCAGACTGGC GTCTGTTTTG CGTGCGTGGA GAGCTGCCAG AGGAGGCTTT GCTGCTAGAG 
GGGGAATGCT GCTGTTCTAG TCTGAGGTCC AGGGGCTCTG TAGTGCTGCT GAACAGCCAG 
CAGGGGGCGC TGTATCTCTG GACTGGCTGT AAAGCCCATA GTGGCGCCCG TGAAGTCAGT 
AAGAGGGCAG TGGAGCGTCT CACTGAAATG AGTCCATTGG AGCTGGGACT CTGCAAAAAC 
AACCCTGTGA AGGTGCACTT TGTGGAGGAG GGTTCAGAAC CTGCAGACTT CTGGACGGCA 
CTGGGCCAGA TGAACAGGAA GGCCTATGAC TGCATGCTGC AAGATCCGGG AAAGTATAAC 
TTCACCCCCC GCCTCTTCCA CCTGAGTGCC TCTTCCGGTA AATTTAAAGC GGAGGAGCTC 
CAGAGTTTGA TCCGGCTGCC GGGGGTCGTG ATGGCCATGC CCTTCGTCCA GGAAAGCTTG 
TACACTGTTC CTCAACCAGC CATGTTCCTG CTTGACAACC GATTAGAGGT CTACCTGTGG 
CAGAGAGGTC AGCCTGAGCA AACGGAGAGC TCCGCTTCAG CCTGGAGCCT GTGGCATAAC 
GAGAGAAAGT GTGCCATGCA GACGGCTCTG CAGTACTGCA AAGAGATAAA CCCGAGACGG 
CCCCCGCAGG CGTATCTCAT CTTAGAGGGA TCAGAGCCTC TCACCTTCAC TAATGTCTTC 
CCCCGCTGGG AGAGGAACCT CGGACCCACC ACACAGGCAT GCACACACAG TTACATAATT 
AGTATTTCCT GCCAGAAAGT AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165410.1
CDS	150..3896
Translation	MSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQEYYKRLIIFQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTAADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309735.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309735.1

ORF Insert Sequence:

ATGAGCATGA GAGAAAAGCT GGCCCTCTTC AACAGAGTGT CCCTGCACGG AAAGAATGGG 
GCCGGCCCCA CCGACGGGCC CCCAGAGAGG CGAAAGCAGA AAGGGGCTCG GTACCGCACC 
CAGCCCATCA CAGTGGACGA AGTCAGCCTG CTTCAGAAAG GCCCCATTCA GCTTCCTGCC 
TTCAGTTTGT CCCCTAATCT GCACGACAGA CAGCAGGCCT CGCCTGTCAA CCTGAAACCC 
AGCGAGGTGC GCCTTATACA ACCAATATCT GAACTTTCAG AACCTGAATC AACGCACCGG 
GGCTCGCGGG ACTGCAACTC CGTACCGCCG TCGAAGGGAA TCCTGAAGAA GAGCTGCCCT 
GAAGGCTCAG AGTGGAGCGG GACCGGCGGT CGGGAGCCGC TACCCAACCA CCAACAAAAT 
GGTAGAGACG TCACCGATGC AGAGATGTTT GGGAGGACGG AGGGAGCTGA GAGGCTGGAA 
TCTCAGAGGG AGACACCGGG CGTGCAGCGA AGTTCAGTCC GAGCAGCTCC GTGGAGGCAG 
AGGGCTCGAA ACCGGAGGGA GACCCCGACA CCGGCCTCCT CAGAGCGGGA TGCTCCTCAG 
GAGGAGAGGC CTTCTCGGAG CGGCCCGCAG CAGGAGATCG CTTCCTCTGT GGGAAACGGC 
TCAGATGTGA CGAGAACACA AGATGAAGAG CAGAGCGACA AACCGAAACA AAACGTTTTG 
GTCTGCGGTG GAGTTCGCTC ACCTAAACAG CAGGAAGCAG CAGCATCTGT GAGAGGCACG 
GAGCCATCAG AAAGCCCTCC TCTTGAGACA CCTCAGTGCT GGGAACCCAT CTTTGCCTCT 
GTCTATTCTA TCAGTACACC CCAATATATC ATGTGTTTTA ACCAGACCAG CCTGTGCTTT 
GAGGCCCAAG AAGTCTCCTC TCCTACTAAG AGCGCAGTCC AGCCCCAGTG GAGGCAGAAG 
GCTAAAGAAG CCGAGGATGA GCTTCAGATG GAAGAAGCTG AAGAGAGGAC CGTGCAGCAG 
CAAAGCAGCG CACAGGAAGC TGCGATCAGC GTCAAGAGAA GTCCGGGATC TCCGGCTGAA 
CCTGACAGTA GGGATGTTTC CAAGAAGAAG ATGGACTCCA GTGAAGCTAC GACAGAGAGT 
GTCTCACACA TGAACCCGGC TCACGAGGAG GCACCAGTCG ATAGTTCCAA GATGTCGGAC 
GGAGGTTTCT GCGGCGGCTG CTTGCCTCCT CCAGCAGGCG GCCCCACACC GTGCGGCGAC 
GTGGCTGCGT CAGAGAGGGA GCGCGACTTT GCCACCCTCT GCCAAAGCAG CATGCCCATA 
CTCAGCTCAG CGGTCGCAGA GCACAGACGA GCAGTGCGTC CGTCTCGTCG GACCCAGGGC 
TCCAGAAACC CCCTTAGGGC TCTGGCAGCC AGAGAGGACA TCAGGCAGGA CTACATGGGG 
GACCGGGACA ACACCGCAGC CGAGGAGAGA GGCCAAGCAG AAAAAAACTG TAAGAACTCT 
TCCCCGCCGG AGTCACTTCC CATCACCTCT TCAGATAAGG AGACAACCAG GACTTTCCCT 
CCGTTCAGCA ACCTGATGCT AATTCACGTC AAGGGGAGGC GGCAGGTCCA GGTACGTCTG 
GTAGAACCCT CGGTCCGTTC GCTGAACAGC GGAGACTGCT TCCTGCTCGT CGCTCCGGAG 
CATTGCATCC TGTGGAGCGG AGAGTTTGCC AACAAAGAGG AGAGGGCAAA GGCCCTTGAA 
CTGGCAGCCT TTATCCAGGG CCATAGGGAC CTCGGCTGTC AGGCCCCCCA GGTGCTCCAC 
CTGGAGGAAG GCTTGAACTC CGATGGCGCC GACGCTGCAG ACTTTTGGAG CCTTCTCGGA 
GGGAGACAAC GTTACAGAGG AGCTGGTGCT CCAGAAGAGG ATGCGCTGTT TGAGCAAGGA 
GTGGTGGAGT CCAACTGTGT TTACAGCCTA GTGGAGGACA GATTGGTGCC TCACGAACGG 
GCCTGGGCCT CCGTCCCCAG CACCTCCCTG CTGGGCTCCT CTGAGGTCCT GGTGTTTGAT 
TTCGGCAGCG AGGTGTATCT GTGGCACGGA CGGGATGTTT CACCCGGCAG GAAGGACGTT 
GCCCTCCAGC TGACTCAGCA GGTGTGGGCG GGCGAGTACG ACTACGGCAA CTGTCGAGTC 
AACCCACTGG ATCCCACTCA GAGCAACCCC AGCGTTCAGC CGAGGGGAGA AGGCCGTCCC 
AGGTGGGCTC TGCTCGCCGT GGTCTCCGAG GACAGCGAGA CGGCTCTTTT CAGGGAAAAG 
TTCCAGGGCC CGACGCGTGG AAGCGGAGAC ACCGTAGAAG TGACTTCAGG GAGAGAAGAG 
ATGCAGGAAT ATTACAAACG ATTGATAATT TTCCAGTCCG TGCTTGCTGA ACCACCCCAG 
CCTCAGTCGT CATCATCGGA CGGCCTGAGT CCCTGTGATG TTAAAGCTCT GGCGTCAGGC 
GGGCTGCTGG AGGAGGGCGT TTCCGTGCGG ACCATTCTGG CTGGGGTCGA CGTTCGTAGA 
GGCTGTGGGG TCATCACCCT GGAGGAGGGC CATCAGATGG AGCTGAGGAC CGTCTCCGTG 
GACACCTGGC ATGTCCAGGA GTTTGACGAC AGTGAAATCC CAGTAGAGAG CACGGGGCAG 
CTGCACGAAG GAGATTCCTA TGTGATCCGC TGGACATACA GCATCAGTGC TGTTGATAAC 
CAGGATGAGT GTGGCAATGC TTCCAAATCT AAGGAAAACA CAGCGTTTTT CTTGTGGCGT 
GGTCGGCACT CCAGCGTCAG CGGAGAAGAC ACAGCAGCTT TTCTATCCAT CGGGATGAAC 
GAGCAGGAAG ACTCACAGGT GGTCGTACCT CAGGGTGCCG AACCCCCCTG CTTCCTGCAG 
CTCTTCCAGG GAGGCCTGGT CATCCACAAG GGGAAGCGAG AGGAGGCCAC CTCCACTGCA 
GCAGACTGGC GTCTGTTTTG CGTGCGTGGA GAGCTGCCAG AGGAGGCTTT GCTGCTAGAG 
GGGGAATGCT GCTGTTCTAG TCTGAGGTCC AGGGGCTCTG TAGTGCTGCT GAACAGCCAG 
CAGGGGGCGC TGTATCTCTG GACTGGCTGT AAAGCCCATA GTGGCGCCCG TGAAGTCAGT 
AAGAGGGCAG TGGAGCGTCT CACTGAAATG AGTCCATTGG AGCTGGGACT CTGCAAAAAC 
AACCCTGTGA AGGTGCACTT TGTGGAGGAG GGTTCAGAAC CTGCAGACTT CTGGACGGCA 
CTGGGCCAGA TGAACAGGAA GGCCTATGAC TGCATGCTGC AAGATCCGGG AAAGTATAAC 
TTCACCCCCC GCCTCTTCCA CCTGAGTGCC TCTTCCGGTA AATTTAAAGC GGAGGAGCTC 
CAGAGTTTGA TCCGGCTGCC GGGGGTCGTG ATGGCCATGC CCTTCGTCCA GGAAAGCTTG 
TACACTGTTC CTCAACCAGC CATGTTCCTG CTTGACAACC GATTAGAGGT CTACCTGTGG 
CAGAGAGGTC AGCCTGAGCA AACGGAGAGC TCCGCTTCAG CCTGGAGCCT GTGGCATAAC 
GAGAGAAAGT GTGCCATGCA GACGGCTCTG CAGTACTGCA AAGAGATAAA CCCGAGACGG 
CCCCCGCAGG CGTATCTCAT CTTAGAGGGA TCAGAGCCTC TCACCTTCAC TAATGTCTTC 
CCCCGCTGGG AGAGGAACCT CGGACCCACC ACACAGGCAT GCACACACAG TTACATAATT 
AGTATTTCCT GCCAGAAAGT AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309730.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165405.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X2, mRNA.

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CloneID	OFu128156
Clone ID Related Accession (Same CDS sequence)	XM_021309730.1 , XM_021309729.1
Accession Version	XM_021309730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GAATATTACA AACGATTGAT AATTTTCCAG TCCGTGCTTG CTGAACCACC CCAGCCTCAG 
TCGTCATCAT CGGACGGCCT GAGTCCCTGT GATGTTAAAG CTCTGGCGTC AGGCGGGCTG 
CTGGAGGAGG GCGTTTCCGT GCGGACCATT CTGGCTGGGG TCGACGTTCG TAGAGGCTGT 
GGGGTCATCA CCCTGGAGGA GGGCCATCAG ATGGAGCTGA GGACCGTCTC CGTGGACACC 
TGGCATGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTAG AGAGCACGGG GCAGCTGCAC 
GAAGGAGATT CCTATGTGAT CCGCTGGACA TACAGCATCA GTGCTGTTGA TAACCAGGAT 
GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA AACACAGCGT TTTTCTTGTG GCGTGGTCGG 
CACTCCAGCG TCAGCGGAGA AGACACAGCA GCTTTTCTAT CCATCGGGAT GAACGAGCAG 
GAAGACTCAC AGGTGGTCGT ACCTCAGGGT GCCGAACCCC CCTGCTTCCT GCAGCTCTTC 
CAGGGAGGCC TGGTCATCCA CAAGGGGAAG CGAGAGGAGG CCACCTCCAC TGCAGCAGAC 
TGGCGTCTGT TTTGCGTGCG TGGAGAGCTG CCAGAGGAGG CTTTGCTGCT AGAGGGGGAA 
TGCTGCTGTT CTAGTCTGAG GTCCAGGGGC TCTGTAGTGC TGCTGAACAG CCAGCAGGGG 
GCGCTGTATC TCTGGACTGG CTGTAAAGCC CATAGTGGCG CCCGTGAAGT CAGTAAGAGG 
GCAGTGGAGC GTCTCACTGA AATGAGTCCA TTGGAGCTGG GACTCTGCAA AAACAACCCT 
GTGAAGGTGC ACTTTGTGGA GGAGGGTTCA GAACCTGCAG ACTTCTGGAC GGCACTGGGC 
CAGATGAACA GGAAGGCCTA TGACTGCATG CTGCAAGATC CGGGAAAGTA TAACTTCACC 
CCCCGCCTCT TCCACCTGAG TGCCTCTTCC GGTAAATTTA AAGCGGAGGA GCTCCAGAGT 
TTGATCCGGC TGCCGGGGGT CGTGATGGCC ATGCCCTTCG TCCAGGAAAG CTTGTACACT 
GTTCCTCAAC CAGCCATGTT CCTGCTTGAC AACCGATTAG AGGTCTACCT GTGGCAGAGA 
GGTCAGCCTG AGCAAACGGA GAGCTCCGCT TCAGCCTGGA GCCTGTGGCA TAACGAGAGA 
AAGTGTGCCA TGCAGACGGC TCTGCAGTAC TGCAAAGAGA TAAACCCGAG ACGGCCCCCG 
CAGGCGTATC TCATCTTAGA GGGATCAGAG CCTCTCACCT TCACTAATGT CTTCCCCCGC 
TGGGAGAGGA ACCTCGGACC CACCACACAG GCATGCACAC ACAGTTACAT AATTAGTATT 
TCCTGCCAGA AAGTAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165405.1
CDS	263..4903
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGQEKLGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQEYYKRLIIFQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTAADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309730.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309730.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GAATATTACA AACGATTGAT AATTTTCCAG TCCGTGCTTG CTGAACCACC CCAGCCTCAG 
TCGTCATCAT CGGACGGCCT GAGTCCCTGT GATGTTAAAG CTCTGGCGTC AGGCGGGCTG 
CTGGAGGAGG GCGTTTCCGT GCGGACCATT CTGGCTGGGG TCGACGTTCG TAGAGGCTGT 
GGGGTCATCA CCCTGGAGGA GGGCCATCAG ATGGAGCTGA GGACCGTCTC CGTGGACACC 
TGGCATGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTAG AGAGCACGGG GCAGCTGCAC 
GAAGGAGATT CCTATGTGAT CCGCTGGACA TACAGCATCA GTGCTGTTGA TAACCAGGAT 
GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA AACACAGCGT TTTTCTTGTG GCGTGGTCGG 
CACTCCAGCG TCAGCGGAGA AGACACAGCA GCTTTTCTAT CCATCGGGAT GAACGAGCAG 
GAAGACTCAC AGGTGGTCGT ACCTCAGGGT GCCGAACCCC CCTGCTTCCT GCAGCTCTTC 
CAGGGAGGCC TGGTCATCCA CAAGGGGAAG CGAGAGGAGG CCACCTCCAC TGCAGCAGAC 
TGGCGTCTGT TTTGCGTGCG TGGAGAGCTG CCAGAGGAGG CTTTGCTGCT AGAGGGGGAA 
TGCTGCTGTT CTAGTCTGAG GTCCAGGGGC TCTGTAGTGC TGCTGAACAG CCAGCAGGGG 
GCGCTGTATC TCTGGACTGG CTGTAAAGCC CATAGTGGCG CCCGTGAAGT CAGTAAGAGG 
GCAGTGGAGC GTCTCACTGA AATGAGTCCA TTGGAGCTGG GACTCTGCAA AAACAACCCT 
GTGAAGGTGC ACTTTGTGGA GGAGGGTTCA GAACCTGCAG ACTTCTGGAC GGCACTGGGC 
CAGATGAACA GGAAGGCCTA TGACTGCATG CTGCAAGATC CGGGAAAGTA TAACTTCACC 
CCCCGCCTCT TCCACCTGAG TGCCTCTTCC GGTAAATTTA AAGCGGAGGA GCTCCAGAGT 
TTGATCCGGC TGCCGGGGGT CGTGATGGCC ATGCCCTTCG TCCAGGAAAG CTTGTACACT 
GTTCCTCAAC CAGCCATGTT CCTGCTTGAC AACCGATTAG AGGTCTACCT GTGGCAGAGA 
GGTCAGCCTG AGCAAACGGA GAGCTCCGCT TCAGCCTGGA GCCTGTGGCA TAACGAGAGA 
AAGTGTGCCA TGCAGACGGC TCTGCAGTAC TGCAAAGAGA TAAACCCGAG ACGGCCCCCG 
CAGGCGTATC TCATCTTAGA GGGATCAGAG CCTCTCACCT TCACTAATGT CTTCCCCCGC 
TGGGAGAGGA ACCTCGGACC CACCACACAG GCATGCACAC ACAGTTACAT AATTAGTATT 
TCCTGCCAGA AAGTAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309729.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165404.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X1, mRNA.

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CloneID	OFu128156
Clone ID Related Accession (Same CDS sequence)	XM_021309730.1 , XM_021309729.1
Accession Version	XM_021309729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GAATATTACA AACGATTGAT AATTTTCCAG TCCGTGCTTG CTGAACCACC CCAGCCTCAG 
TCGTCATCAT CGGACGGCCT GAGTCCCTGT GATGTTAAAG CTCTGGCGTC AGGCGGGCTG 
CTGGAGGAGG GCGTTTCCGT GCGGACCATT CTGGCTGGGG TCGACGTTCG TAGAGGCTGT 
GGGGTCATCA CCCTGGAGGA GGGCCATCAG ATGGAGCTGA GGACCGTCTC CGTGGACACC 
TGGCATGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTAG AGAGCACGGG GCAGCTGCAC 
GAAGGAGATT CCTATGTGAT CCGCTGGACA TACAGCATCA GTGCTGTTGA TAACCAGGAT 
GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA AACACAGCGT TTTTCTTGTG GCGTGGTCGG 
CACTCCAGCG TCAGCGGAGA AGACACAGCA GCTTTTCTAT CCATCGGGAT GAACGAGCAG 
GAAGACTCAC AGGTGGTCGT ACCTCAGGGT GCCGAACCCC CCTGCTTCCT GCAGCTCTTC 
CAGGGAGGCC TGGTCATCCA CAAGGGGAAG CGAGAGGAGG CCACCTCCAC TGCAGCAGAC 
TGGCGTCTGT TTTGCGTGCG TGGAGAGCTG CCAGAGGAGG CTTTGCTGCT AGAGGGGGAA 
TGCTGCTGTT CTAGTCTGAG GTCCAGGGGC TCTGTAGTGC TGCTGAACAG CCAGCAGGGG 
GCGCTGTATC TCTGGACTGG CTGTAAAGCC CATAGTGGCG CCCGTGAAGT CAGTAAGAGG 
GCAGTGGAGC GTCTCACTGA AATGAGTCCA TTGGAGCTGG GACTCTGCAA AAACAACCCT 
GTGAAGGTGC ACTTTGTGGA GGAGGGTTCA GAACCTGCAG ACTTCTGGAC GGCACTGGGC 
CAGATGAACA GGAAGGCCTA TGACTGCATG CTGCAAGATC CGGGAAAGTA TAACTTCACC 
CCCCGCCTCT TCCACCTGAG TGCCTCTTCC GGTAAATTTA AAGCGGAGGA GCTCCAGAGT 
TTGATCCGGC TGCCGGGGGT CGTGATGGCC ATGCCCTTCG TCCAGGAAAG CTTGTACACT 
GTTCCTCAAC CAGCCATGTT CCTGCTTGAC AACCGATTAG AGGTCTACCT GTGGCAGAGA 
GGTCAGCCTG AGCAAACGGA GAGCTCCGCT TCAGCCTGGA GCCTGTGGCA TAACGAGAGA 
AAGTGTGCCA TGCAGACGGC TCTGCAGTAC TGCAAAGAGA TAAACCCGAG ACGGCCCCCG 
CAGGCGTATC TCATCTTAGA GGGATCAGAG CCTCTCACCT TCACTAATGT CTTCCCCCGC 
TGGGAGAGGA ACCTCGGACC CACCACACAG GCATGCACAC ACAGTTACAT AATTAGTATT 
TCCTGCCAGA AAGTAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165404.1
CDS	224..4864
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGQEKLGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQEYYKRLIIFQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTAADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309729.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309729.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GAATATTACA AACGATTGAT AATTTTCCAG TCCGTGCTTG CTGAACCACC CCAGCCTCAG 
TCGTCATCAT CGGACGGCCT GAGTCCCTGT GATGTTAAAG CTCTGGCGTC AGGCGGGCTG 
CTGGAGGAGG GCGTTTCCGT GCGGACCATT CTGGCTGGGG TCGACGTTCG TAGAGGCTGT 
GGGGTCATCA CCCTGGAGGA GGGCCATCAG ATGGAGCTGA GGACCGTCTC CGTGGACACC 
TGGCATGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTAG AGAGCACGGG GCAGCTGCAC 
GAAGGAGATT CCTATGTGAT CCGCTGGACA TACAGCATCA GTGCTGTTGA TAACCAGGAT 
GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA AACACAGCGT TTTTCTTGTG GCGTGGTCGG 
CACTCCAGCG TCAGCGGAGA AGACACAGCA GCTTTTCTAT CCATCGGGAT GAACGAGCAG 
GAAGACTCAC AGGTGGTCGT ACCTCAGGGT GCCGAACCCC CCTGCTTCCT GCAGCTCTTC 
CAGGGAGGCC TGGTCATCCA CAAGGGGAAG CGAGAGGAGG CCACCTCCAC TGCAGCAGAC 
TGGCGTCTGT TTTGCGTGCG TGGAGAGCTG CCAGAGGAGG CTTTGCTGCT AGAGGGGGAA 
TGCTGCTGTT CTAGTCTGAG GTCCAGGGGC TCTGTAGTGC TGCTGAACAG CCAGCAGGGG 
GCGCTGTATC TCTGGACTGG CTGTAAAGCC CATAGTGGCG CCCGTGAAGT CAGTAAGAGG 
GCAGTGGAGC GTCTCACTGA AATGAGTCCA TTGGAGCTGG GACTCTGCAA AAACAACCCT 
GTGAAGGTGC ACTTTGTGGA GGAGGGTTCA GAACCTGCAG ACTTCTGGAC GGCACTGGGC 
CAGATGAACA GGAAGGCCTA TGACTGCATG CTGCAAGATC CGGGAAAGTA TAACTTCACC 
CCCCGCCTCT TCCACCTGAG TGCCTCTTCC GGTAAATTTA AAGCGGAGGA GCTCCAGAGT 
TTGATCCGGC TGCCGGGGGT CGTGATGGCC ATGCCCTTCG TCCAGGAAAG CTTGTACACT 
GTTCCTCAAC CAGCCATGTT CCTGCTTGAC AACCGATTAG AGGTCTACCT GTGGCAGAGA 
GGTCAGCCTG AGCAAACGGA GAGCTCCGCT TCAGCCTGGA GCCTGTGGCA TAACGAGAGA 
AAGTGTGCCA TGCAGACGGC TCTGCAGTAC TGCAAAGAGA TAAACCCGAG ACGGCCCCCG 
CAGGCGTATC TCATCTTAGA GGGATCAGAG CCTCTCACCT TCACTAATGT CTTCCCCCGC 
TGGGAGAGGA ACCTCGGACC CACCACACAG GCATGCACAC ACAGTTACAT AATTAGTATT 
TCCTGCCAGA AAGTAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309731.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165406.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X3, mRNA.

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CloneID	OFu128157
Clone ID Related Accession (Same CDS sequence)	XM_021309731.1
Accession Version	XM_021309731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GAATATTACA AACGATTGAT AATTTTCCAG TCCGTGCTTG CTGAACCACC CCAGCCTCAG 
TCGTCATCAT CGGACGGCCT GAGTCCCTGT GATGTTAAAG CTCTGGCGTC AGGCGGGCTG 
CTGGAGGAGG GCGTTTCCGT GCGGACCATT CTGGCTGGGG TCGACGTTCG TAGAGGCTGT 
GGGGTCATCA CCCTGGAGGA GGGCCATCAG ATGGAGCTGA GGACCGTCTC CGTGGACACC 
TGGCATGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTAG AGAGCACGGG GCAGCTGCAC 
GAAGGAGATT CCTATGTGAT CCGCTGGACA TACAGCATCA GTGCTGTTGA TAACCAGGAT 
GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA AACACAGCGT TTTTCTTGTG GCGTGGTCGG 
CACTCCAGCG TCAGCGGAGA AGACACAGCA GCTTTTCTAT CCATCGGGAT GAACGAGCAG 
GAAGACTCAC AGGTGGTCGT ACCTCAGGGT GCCGAACCCC CCTGCTTCCT GCAGCTCTTC 
CAGGGAGGCC TGGTCATCCA CAAGGGGAAG CGAGAGGAGG CCACCTCCAC TGCAGACTGG 
CGTCTGTTTT GCGTGCGTGG AGAGCTGCCA GAGGAGGCTT TGCTGCTAGA GGGGGAATGC 
TGCTGTTCTA GTCTGAGGTC CAGGGGCTCT GTAGTGCTGC TGAACAGCCA GCAGGGGGCG 
CTGTATCTCT GGACTGGCTG TAAAGCCCAT AGTGGCGCCC GTGAAGTCAG TAAGAGGGCA 
GTGGAGCGTC TCACTGAAAT GAGTCCATTG GAGCTGGGAC TCTGCAAAAA CAACCCTGTG 
AAGGTGCACT TTGTGGAGGA GGGTTCAGAA CCTGCAGACT TCTGGACGGC ACTGGGCCAG 
ATGAACAGGA AGGCCTATGA CTGCATGCTG CAAGATCCGG GAAAGTATAA CTTCACCCCC 
CGCCTCTTCC ACCTGAGTGC CTCTTCCGGT AAATTTAAAG CGGAGGAGCT CCAGAGTTTG 
ATCCGGCTGC CGGGGGTCGT GATGGCCATG CCCTTCGTCC AGGAAAGCTT GTACACTGTT 
CCTCAACCAG CCATGTTCCT GCTTGACAAC CGATTAGAGG TCTACCTGTG GCAGAGAGGT 
CAGCCTGAGC AAACGGAGAG CTCCGCTTCA GCCTGGAGCC TGTGGCATAA CGAGAGAAAG 
TGTGCCATGC AGACGGCTCT GCAGTACTGC AAAGAGATAA ACCCGAGACG GCCCCCGCAG 
GCGTATCTCA TCTTAGAGGG ATCAGAGCCT CTCACCTTCA CTAATGTCTT CCCCCGCTGG 
GAGAGGAACC TCGGACCCAC CACACAGGCA TGCACACACA GTTACATAAT TAGTATTTCC 
TGCCAGAAAG TAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165406.1
CDS	224..4861
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGQEKLGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQEYYKRLIIFQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309731.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309731.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GAATATTACA AACGATTGAT AATTTTCCAG TCCGTGCTTG CTGAACCACC CCAGCCTCAG 
TCGTCATCAT CGGACGGCCT GAGTCCCTGT GATGTTAAAG CTCTGGCGTC AGGCGGGCTG 
CTGGAGGAGG GCGTTTCCGT GCGGACCATT CTGGCTGGGG TCGACGTTCG TAGAGGCTGT 
GGGGTCATCA CCCTGGAGGA GGGCCATCAG ATGGAGCTGA GGACCGTCTC CGTGGACACC 
TGGCATGTCC AGGAGTTTGA CGACAGTGAA ATCCCAGTAG AGAGCACGGG GCAGCTGCAC 
GAAGGAGATT CCTATGTGAT CCGCTGGACA TACAGCATCA GTGCTGTTGA TAACCAGGAT 
GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA AACACAGCGT TTTTCTTGTG GCGTGGTCGG 
CACTCCAGCG TCAGCGGAGA AGACACAGCA GCTTTTCTAT CCATCGGGAT GAACGAGCAG 
GAAGACTCAC AGGTGGTCGT ACCTCAGGGT GCCGAACCCC CCTGCTTCCT GCAGCTCTTC 
CAGGGAGGCC TGGTCATCCA CAAGGGGAAG CGAGAGGAGG CCACCTCCAC TGCAGACTGG 
CGTCTGTTTT GCGTGCGTGG AGAGCTGCCA GAGGAGGCTT TGCTGCTAGA GGGGGAATGC 
TGCTGTTCTA GTCTGAGGTC CAGGGGCTCT GTAGTGCTGC TGAACAGCCA GCAGGGGGCG 
CTGTATCTCT GGACTGGCTG TAAAGCCCAT AGTGGCGCCC GTGAAGTCAG TAAGAGGGCA 
GTGGAGCGTC TCACTGAAAT GAGTCCATTG GAGCTGGGAC TCTGCAAAAA CAACCCTGTG 
AAGGTGCACT TTGTGGAGGA GGGTTCAGAA CCTGCAGACT TCTGGACGGC ACTGGGCCAG 
ATGAACAGGA AGGCCTATGA CTGCATGCTG CAAGATCCGG GAAAGTATAA CTTCACCCCC 
CGCCTCTTCC ACCTGAGTGC CTCTTCCGGT AAATTTAAAG CGGAGGAGCT CCAGAGTTTG 
ATCCGGCTGC CGGGGGTCGT GATGGCCATG CCCTTCGTCC AGGAAAGCTT GTACACTGTT 
CCTCAACCAG CCATGTTCCT GCTTGACAAC CGATTAGAGG TCTACCTGTG GCAGAGAGGT 
CAGCCTGAGC AAACGGAGAG CTCCGCTTCA GCCTGGAGCC TGTGGCATAA CGAGAGAAAG 
TGTGCCATGC AGACGGCTCT GCAGTACTGC AAAGAGATAA ACCCGAGACG GCCCCCGCAG 
GCGTATCTCA TCTTAGAGGG ATCAGAGCCT CTCACCTTCA CTAATGTCTT CCCCCGCTGG 
GAGAGGAACC TCGGACCCAC CACACAGGCA TGCACACACA GTTACATAAT TAGTATTTCC 
TGCCAGAAAG TAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309732.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165407.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFu128158
Clone ID Related Accession (Same CDS sequence)	XM_021309732.1
Accession Version	XM_021309732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GTTAACCTCA TATCCGTGCT TGCTGAACCA CCCCAGCCTC AGTCGTCATC ATCGGACGGC 
CTGAGTCCCT GTGATGTTAA AGCTCTGGCG TCAGGCGGGC TGCTGGAGGA GGGCGTTTCC 
GTGCGGACCA TTCTGGCTGG GGTCGACGTT CGTAGAGGCT GTGGGGTCAT CACCCTGGAG 
GAGGGCCATC AGATGGAGCT GAGGACCGTC TCCGTGGACA CCTGGCATGT CCAGGAGTTT 
GACGACAGTG AAATCCCAGT AGAGAGCACG GGGCAGCTGC ACGAAGGAGA TTCCTATGTG 
ATCCGCTGGA CATACAGCAT CAGTGCTGTT GATAACCAGG ATGAGTGTGG CAATGCTTCC 
AAATCTAAGG AAAACACAGC GTTTTTCTTG TGGCGTGGTC GGCACTCCAG CGTCAGCGGA 
GAAGACACAG CAGCTTTTCT ATCCATCGGG ATGAACGAGC AGGAAGACTC ACAGGTGGTC 
GTACCTCAGG GTGCCGAACC CCCCTGCTTC CTGCAGCTCT TCCAGGGAGG CCTGGTCATC 
CACAAGGGGA AGCGAGAGGA GGCCACCTCC ACTGCAGCAG ACTGGCGTCT GTTTTGCGTG 
CGTGGAGAGC TGCCAGAGGA GGCTTTGCTG CTAGAGGGGG AATGCTGCTG TTCTAGTCTG 
AGGTCCAGGG GCTCTGTAGT GCTGCTGAAC AGCCAGCAGG GGGCGCTGTA TCTCTGGACT 
GGCTGTAAAG CCCATAGTGG CGCCCGTGAA GTCAGTAAGA GGGCAGTGGA GCGTCTCACT 
GAAATGAGTC CATTGGAGCT GGGACTCTGC AAAAACAACC CTGTGAAGGT GCACTTTGTG 
GAGGAGGGTT CAGAACCTGC AGACTTCTGG ACGGCACTGG GCCAGATGAA CAGGAAGGCC 
TATGACTGCA TGCTGCAAGA TCCGGGAAAG TATAACTTCA CCCCCCGCCT CTTCCACCTG 
AGTGCCTCTT CCGGTAAATT TAAAGCGGAG GAGCTCCAGA GTTTGATCCG GCTGCCGGGG 
GTCGTGATGG CCATGCCCTT CGTCCAGGAA AGCTTGTACA CTGTTCCTCA ACCAGCCATG 
TTCCTGCTTG ACAACCGATT AGAGGTCTAC CTGTGGCAGA GAGGTCAGCC TGAGCAAACG 
GAGAGCTCCG CTTCAGCCTG GAGCCTGTGG CATAACGAGA GAAAGTGTGC CATGCAGACG 
GCTCTGCAGT ACTGCAAAGA GATAAACCCG AGACGGCCCC CGCAGGCGTA TCTCATCTTA 
GAGGGATCAG AGCCTCTCAC CTTCACTAAT GTCTTCCCCC GCTGGGAGAG GAACCTCGGA 
CCCACCACAC AGGCATGCAC ACACAGTTAC ATAATTAGTA TTTCCTGCCA GAAAGTAGGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165407.1
CDS	224..4846
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGQEKLGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQVNLISVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTAADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309732.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309732.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
GTTAACCTCA TATCCGTGCT TGCTGAACCA CCCCAGCCTC AGTCGTCATC ATCGGACGGC 
CTGAGTCCCT GTGATGTTAA AGCTCTGGCG TCAGGCGGGC TGCTGGAGGA GGGCGTTTCC 
GTGCGGACCA TTCTGGCTGG GGTCGACGTT CGTAGAGGCT GTGGGGTCAT CACCCTGGAG 
GAGGGCCATC AGATGGAGCT GAGGACCGTC TCCGTGGACA CCTGGCATGT CCAGGAGTTT 
GACGACAGTG AAATCCCAGT AGAGAGCACG GGGCAGCTGC ACGAAGGAGA TTCCTATGTG 
ATCCGCTGGA CATACAGCAT CAGTGCTGTT GATAACCAGG ATGAGTGTGG CAATGCTTCC 
AAATCTAAGG AAAACACAGC GTTTTTCTTG TGGCGTGGTC GGCACTCCAG CGTCAGCGGA 
GAAGACACAG CAGCTTTTCT ATCCATCGGG ATGAACGAGC AGGAAGACTC ACAGGTGGTC 
GTACCTCAGG GTGCCGAACC CCCCTGCTTC CTGCAGCTCT TCCAGGGAGG CCTGGTCATC 
CACAAGGGGA AGCGAGAGGA GGCCACCTCC ACTGCAGCAG ACTGGCGTCT GTTTTGCGTG 
CGTGGAGAGC TGCCAGAGGA GGCTTTGCTG CTAGAGGGGG AATGCTGCTG TTCTAGTCTG 
AGGTCCAGGG GCTCTGTAGT GCTGCTGAAC AGCCAGCAGG GGGCGCTGTA TCTCTGGACT 
GGCTGTAAAG CCCATAGTGG CGCCCGTGAA GTCAGTAAGA GGGCAGTGGA GCGTCTCACT 
GAAATGAGTC CATTGGAGCT GGGACTCTGC AAAAACAACC CTGTGAAGGT GCACTTTGTG 
GAGGAGGGTT CAGAACCTGC AGACTTCTGG ACGGCACTGG GCCAGATGAA CAGGAAGGCC 
TATGACTGCA TGCTGCAAGA TCCGGGAAAG TATAACTTCA CCCCCCGCCT CTTCCACCTG 
AGTGCCTCTT CCGGTAAATT TAAAGCGGAG GAGCTCCAGA GTTTGATCCG GCTGCCGGGG 
GTCGTGATGG CCATGCCCTT CGTCCAGGAA AGCTTGTACA CTGTTCCTCA ACCAGCCATG 
TTCCTGCTTG ACAACCGATT AGAGGTCTAC CTGTGGCAGA GAGGTCAGCC TGAGCAAACG 
GAGAGCTCCG CTTCAGCCTG GAGCCTGTGG CATAACGAGA GAAAGTGTGC CATGCAGACG 
GCTCTGCAGT ACTGCAAAGA GATAAACCCG AGACGGCCCC CGCAGGCGTA TCTCATCTTA 
GAGGGATCAG AGCCTCTCAC CTTCACTAAT GTCTTCCCCC GCTGGGAGAG GAACCTCGGA 
CCCACCACAC AGGCATGCAC ACACAGTTAC ATAATTAGTA TTTCCTGCCA GAAAGTAGGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309733.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165408.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X5, mRNA.

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CloneID	OFu128159
Clone ID Related Accession (Same CDS sequence)	XM_021309733.1
Accession Version	XM_021309733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
TCCGTGCTTG CTGAACCACC CCAGCCTCAG TCGTCATCAT CGGACGGCCT GAGTCCCTGT 
GATGTTAAAG CTCTGGCGTC AGGCGGGCTG CTGGAGGAGG GCGTTTCCGT GCGGACCATT 
CTGGCTGGGG TCGACGTTCG TAGAGGCTGT GGGGTCATCA CCCTGGAGGA GGGCCATCAG 
ATGGAGCTGA GGACCGTCTC CGTGGACACC TGGCATGTCC AGGAGTTTGA CGACAGTGAA 
ATCCCAGTAG AGAGCACGGG GCAGCTGCAC GAAGGAGATT CCTATGTGAT CCGCTGGACA 
TACAGCATCA GTGCTGTTGA TAACCAGGAT GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA 
AACACAGCGT TTTTCTTGTG GCGTGGTCGG CACTCCAGCG TCAGCGGAGA AGACACAGCA 
GCTTTTCTAT CCATCGGGAT GAACGAGCAG GAAGACTCAC AGGTGGTCGT ACCTCAGGGT 
GCCGAACCCC CCTGCTTCCT GCAGCTCTTC CAGGGAGGCC TGGTCATCCA CAAGGGGAAG 
CGAGAGGAGG CCACCTCCAC TGCAGCAGAC TGGCGTCTGT TTTGCGTGCG TGGAGAGCTG 
CCAGAGGAGG CTTTGCTGCT AGAGGGGGAA TGCTGCTGTT CTAGTCTGAG GTCCAGGGGC 
TCTGTAGTGC TGCTGAACAG CCAGCAGGGG GCGCTGTATC TCTGGACTGG CTGTAAAGCC 
CATAGTGGCG CCCGTGAAGT CAGTAAGAGG GCAGTGGAGC GTCTCACTGA AATGAGTCCA 
TTGGAGCTGG GACTCTGCAA AAACAACCCT GTGAAGGTGC ACTTTGTGGA GGAGGGTTCA 
GAACCTGCAG ACTTCTGGAC GGCACTGGGC CAGATGAACA GGAAGGCCTA TGACTGCATG 
CTGCAAGATC CGGGAAAGTA TAACTTCACC CCCCGCCTCT TCCACCTGAG TGCCTCTTCC 
GGTAAATTTA AAGCGGAGGA GCTCCAGAGT TTGATCCGGC TGCCGGGGGT CGTGATGGCC 
ATGCCCTTCG TCCAGGAAAG CTTGTACACT GTTCCTCAAC CAGCCATGTT CCTGCTTGAC 
AACCGATTAG AGGTCTACCT GTGGCAGAGA GGTCAGCCTG AGCAAACGGA GAGCTCCGCT 
TCAGCCTGGA GCCTGTGGCA TAACGAGAGA AAGTGTGCCA TGCAGACGGC TCTGCAGTAC 
TGCAAAGAGA TAAACCCGAG ACGGCCCCCG CAGGCGTATC TCATCTTAGA GGGATCAGAG 
CCTCTCACCT TCACTAATGT CTTCCCCCGC TGGGAGAGGA ACCTCGGACC CACCACACAG 
GCATGCACAC ACAGTTACAT AATTAGTATT TCCTGCCAGA AAGTAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165408.1
CDS	224..4834
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGQEKLGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWEPIFASVYSISTPQYIMCFNQTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTAADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309733.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309733.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGGAAC CCATCTTTGC CTCTGTCTAT 
TCTATCAGTA CACCCCAATA TATCATGTGT TTTAACCAGA CCAGCCTGTG CTTTGAGGCC 
CAAGAAGTCT CCTCTCCTAC TAAGAGCGCA GTCCAGCCCC AGTGGAGGCA GAAGGCTAAA 
GAAGCCGAGG ATGAGCTTCA GATGGAAGAA GCTGAAGAGA GGACCGTGCA GCAGCAAAGC 
AGCGCACAGG AAGCTGCGAT CAGCGTCAAG AGAAGTCCGG GATCTCCGGC TGAACCTGAC 
AGTAGGGATG TTTCCAAGAA GAAGATGGAC TCCAGTGAAG CTACGACAGA GAGTGTCTCA 
CACATGAACC CGGCTCACGA GGAGGCACCA GTCGATAGTT CCAAGATGTC GGACGGAGGT 
TTCTGCGGCG GCTGCTTGCC TCCTCCAGCA GGCGGCCCCA CACCGTGCGG CGACGTGGCT 
GCGTCAGAGA GGGAGCGCGA CTTTGCCACC CTCTGCCAAA GCAGCATGCC CATACTCAGC 
TCAGCGGTCG CAGAGCACAG ACGAGCAGTG CGTCCGTCTC GTCGGACCCA GGGCTCCAGA 
AACCCCCTTA GGGCTCTGGC AGCCAGAGAG GACATCAGGC AGGACTACAT GGGGGACCGG 
GACAACACCG CAGCCGAGGA GAGAGGCCAA GCAGAAAAAA ACTGTAAGAA CTCTTCCCCG 
CCGGAGTCAC TTCCCATCAC CTCTTCAGAT AAGGAGACAA CCAGGACTTT CCCTCCGTTC 
AGCAACCTGA TGCTAATTCA CGTCAAGGGG AGGCGGCAGG TCCAGGTACG TCTGGTAGAA 
CCCTCGGTCC GTTCGCTGAA CAGCGGAGAC TGCTTCCTGC TCGTCGCTCC GGAGCATTGC 
ATCCTGTGGA GCGGAGAGTT TGCCAACAAA GAGGAGAGGG CAAAGGCCCT TGAACTGGCA 
GCCTTTATCC AGGGCCATAG GGACCTCGGC TGTCAGGCCC CCCAGGTGCT CCACCTGGAG 
GAAGGCTTGA ACTCCGATGG CGCCGACGCT GCAGACTTTT GGAGCCTTCT CGGAGGGAGA 
CAACGTTACA GAGGAGCTGG TGCTCCAGAA GAGGATGCGC TGTTTGAGCA AGGAGTGGTG 
GAGTCCAACT GTGTTTACAG CCTAGTGGAG GACAGATTGG TGCCTCACGA ACGGGCCTGG 
GCCTCCGTCC CCAGCACCTC CCTGCTGGGC TCCTCTGAGG TCCTGGTGTT TGATTTCGGC 
AGCGAGGTGT ATCTGTGGCA CGGACGGGAT GTTTCACCCG GCAGGAAGGA CGTTGCCCTC 
CAGCTGACTC AGCAGGTGTG GGCGGGCGAG TACGACTACG GCAACTGTCG AGTCAACCCA 
CTGGATCCCA CTCAGAGCAA CCCCAGCGTT CAGCCGAGGG GAGAAGGCCG TCCCAGGTGG 
GCTCTGCTCG CCGTGGTCTC CGAGGACAGC GAGACGGCTC TTTTCAGGGA AAAGTTCCAG 
GGCCCGACGC GTGGAAGCGG AGACACCGTA GAAGTGACTT CAGGGAGAGA AGAGATGCAG 
TCCGTGCTTG CTGAACCACC CCAGCCTCAG TCGTCATCAT CGGACGGCCT GAGTCCCTGT 
GATGTTAAAG CTCTGGCGTC AGGCGGGCTG CTGGAGGAGG GCGTTTCCGT GCGGACCATT 
CTGGCTGGGG TCGACGTTCG TAGAGGCTGT GGGGTCATCA CCCTGGAGGA GGGCCATCAG 
ATGGAGCTGA GGACCGTCTC CGTGGACACC TGGCATGTCC AGGAGTTTGA CGACAGTGAA 
ATCCCAGTAG AGAGCACGGG GCAGCTGCAC GAAGGAGATT CCTATGTGAT CCGCTGGACA 
TACAGCATCA GTGCTGTTGA TAACCAGGAT GAGTGTGGCA ATGCTTCCAA ATCTAAGGAA 
AACACAGCGT TTTTCTTGTG GCGTGGTCGG CACTCCAGCG TCAGCGGAGA AGACACAGCA 
GCTTTTCTAT CCATCGGGAT GAACGAGCAG GAAGACTCAC AGGTGGTCGT ACCTCAGGGT 
GCCGAACCCC CCTGCTTCCT GCAGCTCTTC CAGGGAGGCC TGGTCATCCA CAAGGGGAAG 
CGAGAGGAGG CCACCTCCAC TGCAGCAGAC TGGCGTCTGT TTTGCGTGCG TGGAGAGCTG 
CCAGAGGAGG CTTTGCTGCT AGAGGGGGAA TGCTGCTGTT CTAGTCTGAG GTCCAGGGGC 
TCTGTAGTGC TGCTGAACAG CCAGCAGGGG GCGCTGTATC TCTGGACTGG CTGTAAAGCC 
CATAGTGGCG CCCGTGAAGT CAGTAAGAGG GCAGTGGAGC GTCTCACTGA AATGAGTCCA 
TTGGAGCTGG GACTCTGCAA AAACAACCCT GTGAAGGTGC ACTTTGTGGA GGAGGGTTCA 
GAACCTGCAG ACTTCTGGAC GGCACTGGGC CAGATGAACA GGAAGGCCTA TGACTGCATG 
CTGCAAGATC CGGGAAAGTA TAACTTCACC CCCCGCCTCT TCCACCTGAG TGCCTCTTCC 
GGTAAATTTA AAGCGGAGGA GCTCCAGAGT TTGATCCGGC TGCCGGGGGT CGTGATGGCC 
ATGCCCTTCG TCCAGGAAAG CTTGTACACT GTTCCTCAAC CAGCCATGTT CCTGCTTGAC 
AACCGATTAG AGGTCTACCT GTGGCAGAGA GGTCAGCCTG AGCAAACGGA GAGCTCCGCT 
TCAGCCTGGA GCCTGTGGCA TAACGAGAGA AAGTGTGCCA TGCAGACGGC TCTGCAGTAC 
TGCAAAGAGA TAAACCCGAG ACGGCCCCCG CAGGCGTATC TCATCTTAGA GGGATCAGAG 
CCTCTCACCT TCACTAATGT CTTCCCCCGC TGGGAGAGGA ACCTCGGACC CACCACACAG 
GCATGCACAC ACAGTTACAT AATTAGTATT TCCTGCCAGA AAGTAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105916477 ( XM_021309734.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021165409.1 Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X6, mRNA.

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CloneID	OFu128160
Clone ID Related Accession (Same CDS sequence)	XM_021309734.1
Accession Version	XM_021309734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	supervillin-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224580.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGACCA GCCTGTGCTT TGAGGCCCAA 
GAAGTCTCCT CTCCTACTAA GAGCGCAGTC CAGCCCCAGT GGAGGCAGAA GGCTAAAGAA 
GCCGAGGATG AGCTTCAGAT GGAAGAAGCT GAAGAGAGGA CCGTGCAGCA GCAAAGCAGC 
GCACAGGAAG CTGCGATCAG CGTCAAGAGA AGTCCGGGAT CTCCGGCTGA ACCTGACAGT 
AGGGATGTTT CCAAGAAGAA GATGGACTCC AGTGAAGCTA CGACAGAGAG TGTCTCACAC 
ATGAACCCGG CTCACGAGGA GGCACCAGTC GATAGTTCCA AGATGTCGGA CGGAGGTTTC 
TGCGGCGGCT GCTTGCCTCC TCCAGCAGGC GGCCCCACAC CGTGCGGCGA CGTGGCTGCG 
TCAGAGAGGG AGCGCGACTT TGCCACCCTC TGCCAAAGCA GCATGCCCAT ACTCAGCTCA 
GCGGTCGCAG AGCACAGACG AGCAGTGCGT CCGTCTCGTC GGACCCAGGG CTCCAGAAAC 
CCCCTTAGGG CTCTGGCAGC CAGAGAGGAC ATCAGGCAGG ACTACATGGG GGACCGGGAC 
AACACCGCAG CCGAGGAGAG AGGCCAAGCA GAAAAAAACT GTAAGAACTC TTCCCCGCCG 
GAGTCACTTC CCATCACCTC TTCAGATAAG GAGACAACCA GGACTTTCCC TCCGTTCAGC 
AACCTGATGC TAATTCACGT CAAGGGGAGG CGGCAGGTCC AGGTACGTCT GGTAGAACCC 
TCGGTCCGTT CGCTGAACAG CGGAGACTGC TTCCTGCTCG TCGCTCCGGA GCATTGCATC 
CTGTGGAGCG GAGAGTTTGC CAACAAAGAG GAGAGGGCAA AGGCCCTTGA ACTGGCAGCC 
TTTATCCAGG GCCATAGGGA CCTCGGCTGT CAGGCCCCCC AGGTGCTCCA CCTGGAGGAA 
GGCTTGAACT CCGATGGCGC CGACGCTGCA GACTTTTGGA GCCTTCTCGG AGGGAGACAA 
CGTTACAGAG GAGCTGGTGC TCCAGAAGAG GATGCGCTGT TTGAGCAAGG AGTGGTGGAG 
TCCAACTGTG TTTACAGCCT AGTGGAGGAC AGATTGGTGC CTCACGAACG GGCCTGGGCC 
TCCGTCCCCA GCACCTCCCT GCTGGGCTCC TCTGAGGTCC TGGTGTTTGA TTTCGGCAGC 
GAGGTGTATC TGTGGCACGG ACGGGATGTT TCACCCGGCA GGAAGGACGT TGCCCTCCAG 
CTGACTCAGC AGGTGTGGGC GGGCGAGTAC GACTACGGCA ACTGTCGAGT CAACCCACTG 
GATCCCACTC AGAGCAACCC CAGCGTTCAG CCGAGGGGAG AAGGCCGTCC CAGGTGGGCT 
CTGCTCGCCG TGGTCTCCGA GGACAGCGAG ACGGCTCTTT TCAGGGAAAA GTTCCAGGGC 
CCGACGCGTG GAAGCGGAGA CACCGTAGAA GTGACTTCAG GGAGAGAAGA GATGCAGGAA 
TATTACAAAC GATTGATAAT TTTCCAGTCC GTGCTTGCTG AACCACCCCA GCCTCAGTCG 
TCATCATCGG ACGGCCTGAG TCCCTGTGAT GTTAAAGCTC TGGCGTCAGG CGGGCTGCTG 
GAGGAGGGCG TTTCCGTGCG GACCATTCTG GCTGGGGTCG ACGTTCGTAG AGGCTGTGGG 
GTCATCACCC TGGAGGAGGG CCATCAGATG GAGCTGAGGA CCGTCTCCGT GGACACCTGG 
CATGTCCAGG AGTTTGACGA CAGTGAAATC CCAGTAGAGA GCACGGGGCA GCTGCACGAA 
GGAGATTCCT ATGTGATCCG CTGGACATAC AGCATCAGTG CTGTTGATAA CCAGGATGAG 
TGTGGCAATG CTTCCAAATC TAAGGAAAAC ACAGCGTTTT TCTTGTGGCG TGGTCGGCAC 
TCCAGCGTCA GCGGAGAAGA CACAGCAGCT TTTCTATCCA TCGGGATGAA CGAGCAGGAA 
GACTCACAGG TGGTCGTACC TCAGGGTGCC GAACCCCCCT GCTTCCTGCA GCTCTTCCAG 
GGAGGCCTGG TCATCCACAA GGGGAAGCGA GAGGAGGCCA CCTCCACTGC AGCAGACTGG 
CGTCTGTTTT GCGTGCGTGG AGAGCTGCCA GAGGAGGCTT TGCTGCTAGA GGGGGAATGC 
TGCTGTTCTA GTCTGAGGTC CAGGGGCTCT GTAGTGCTGC TGAACAGCCA GCAGGGGGCG 
CTGTATCTCT GGACTGGCTG TAAAGCCCAT AGTGGCGCCC GTGAAGTCAG TAAGAGGGCA 
GTGGAGCGTC TCACTGAAAT GAGTCCATTG GAGCTGGGAC TCTGCAAAAA CAACCCTGTG 
AAGGTGCACT TTGTGGAGGA GGGTTCAGAA CCTGCAGACT TCTGGACGGC ACTGGGCCAG 
ATGAACAGGA AGGCCTATGA CTGCATGCTG CAAGATCCGG GAAAGTATAA CTTCACCCCC 
CGCCTCTTCC ACCTGAGTGC CTCTTCCGGT AAATTTAAAG CGGAGGAGCT CCAGAGTTTG 
ATCCGGCTGC CGGGGGTCGT GATGGCCATG CCCTTCGTCC AGGAAAGCTT GTACACTGTT 
CCTCAACCAG CCATGTTCCT GCTTGACAAC CGATTAGAGG TCTACCTGTG GCAGAGAGGT 
CAGCCTGAGC AAACGGAGAG CTCCGCTTCA GCCTGGAGCC TGTGGCATAA CGAGAGAAAG 
TGTGCCATGC AGACGGCTCT GCAGTACTGC AAAGAGATAA ACCCGAGACG GCCCCCGCAG 
GCGTATCTCA TCTTAGAGGG ATCAGAGCCT CTCACCTTCA CTAATGTCTT CCCCCGCTGG 
GAGAGGAACC TCGGACCCAC CACACAGGCA TGCACACACA GTTACATAAT TAGTATTTCC 
TGCCAGAAAG TAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021165409.1
CDS	225..4802
Translation	MDATEKQALEPRAERIARYKAERRRELAELYGDLEELPSKWVRRDGKKVNNPATPARGGALNSDGRGEGVNGRTREVTNGVEADPPTEPSYLRSQSYQDSATVLVEDAPQLHTRVSVGQLKSVLLQQAESGAGQEKLGPDIGQTTSSLDLAAGSEGSRRRTRRYLPGASGGGRKTSERFRTQPITANEMEERTGLLDAEVNDNKKADVKTDDRAKMSVAAKMSLFKELEKSAAPDSSALLKPRSASSFQERRTRRGNEHRFLTQPITCEEMVAISAPPAEEVQTEQAEPVEAADESCRMSMREKLALFNRVSLHGKNGAGPTDGPPERRKQKGARYRTQPITVDEVSLLQKGPIQLPAFSLSPNLHDRQQASPVNLKPSEVRLIQPISELSEPESTHRGSRDCNSVPPSKGILKKSCPEGSEWSGTGGREPLPNHQQNGRDVTDAEMFGRTEGAERLESQRETPGVQRSSVRAAPWRQRARNRRETPTPASSERDAPQEERPSRSGPQQEIASSVGNGSDVTRTQDEEQSDKPKQNVLVCGGVRSPKQQEAAASVRGTEPSESPPLETPQCWTSLCFEAQEVSSPTKSAVQPQWRQKAKEAEDELQMEEAEERTVQQQSSAQEAAISVKRSPGSPAEPDSRDVSKKKMDSSEATTESVSHMNPAHEEAPVDSSKMSDGGFCGGCLPPPAGGPTPCGDVAASERERDFATLCQSSMPILSSAVAEHRRAVRPSRRTQGSRNPLRALAAREDIRQDYMGDRDNTAAEERGQAEKNCKNSSPPESLPITSSDKETTRTFPPFSNLMLIHVKGRRQVQVRLVEPSVRSLNSGDCFLLVAPEHCILWSGEFANKEERAKALELAAFIQGHRDLGCQAPQVLHLEEGLNSDGADAADFWSLLGGRQRYRGAGAPEEDALFEQGVVESNCVYSLVEDRLVPHERAWASVPSTSLLGSSEVLVFDFGSEVYLWHGRDVSPGRKDVALQLTQQVWAGEYDYGNCRVNPLDPTQSNPSVQPRGEGRPRWALLAVVSEDSETALFREKFQGPTRGSGDTVEVTSGREEMQEYYKRLIIFQSVLAEPPQPQSSSSDGLSPCDVKALASGGLLEEGVSVRTILAGVDVRRGCGVITLEEGHQMELRTVSVDTWHVQEFDDSEIPVESTGQLHEGDSYVIRWTYSISAVDNQDECGNASKSKENTAFFLWRGRHSSVSGEDTAAFLSIGMNEQEDSQVVVPQGAEPPCFLQLFQGGLVIHKGKREEATSTAADWRLFCVRGELPEEALLLEGECCCSSLRSRGSVVLLNSQQGALYLWTGCKAHSGAREVSKRAVERLTEMSPLELGLCKNNPVKVHFVEEGSEPADFWTALGQMNRKAYDCMLQDPGKYNFTPRLFHLSASSGKFKAEELQSLIRLPGVVMAMPFVQESLYTVPQPAMFLLDNRLEVYLWQRGQPEQTESSASAWSLWHNERKCAMQTALQYCKEINPRRPPQAYLILEGSEPLTFTNVFPRWERNLGPTTQACTHSYIISISCQKVG

Target ORF information:

RefSeq Version	XM_021309734.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus supervillin-like (LOC105916477), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309734.1

ORF Insert Sequence:

ATGGATGCAA CGGAAAAGCA GGCTCTGGAG CCCAGAGCAG AGCGGATCGC CCGCTACAAG 
GCCGAGAGGA GGCGAGAGCT GGCCGAGCTC TACGGAGACT TAGAGGAGCT TCCCTCCAAG 
TGGGTGAGAC GGGATGGAAA GAAGGTCAAC AACCCAGCAA CCCCAGCCCG CGGCGGGGCG 
TTAAACTCAG ACGGCCGCGG TGAAGGGGTG AACGGCAGAA CACGAGAAGT TACAAATGGA 
GTCGAGGCGG ATCCTCCGAC AGAGCCCAGC TACCTGAGAA GCCAGAGTTA CCAAGATTCT 
GCCACCGTCC TGGTCGAGGA TGCCCCACAG CTCCACACCC GGGTGTCGGT GGGCCAGTTG 
AAGAGTGTCC TTCTGCAGCA GGCAGAGAGC GGAGCAGGCC AGGAGAAACT TGGTCCTGAT 
ATTGGCCAAA CCACGTCTTC TTTGGACCTG GCTGCTGGTT CTGAGGGAAG CCGGCGGCGG 
ACCAGGCGTT ACCTTCCAGG CGCATCAGGA GGGGGTCGCA AAACCAGCGA GCGCTTCAGG 
ACCCAGCCAA TCACAGCCAA TGAGATGGAG GAGAGAACAG GGCTCTTGGA CGCGGAAGTA 
AATGACAACA AGAAAGCCGA TGTGAAAACA GATGATCGAG CCAAAATGAG CGTGGCAGCC 
AAGATGTCGC TGTTCAAAGA ATTGGAGAAG TCAGCGGCCC CCGATTCCTC GGCTCTGCTG 
AAGCCTCGCT CAGCCAGCAG CTTCCAGGAA CGGAGGACGC GCCGTGGCAA CGAGCATCGC 
TTTCTCACTC AGCCAATCAC CTGTGAGGAA ATGGTGGCAA TCAGCGCCCC TCCAGCAGAG 
GAGGTCCAGA CCGAGCAGGC CGAACCGGTG GAGGCCGCAG ATGAAAGCTG CCGGATGAGC 
ATGAGAGAAA AGCTGGCCCT CTTCAACAGA GTGTCCCTGC ACGGAAAGAA TGGGGCCGGC 
CCCACCGACG GGCCCCCAGA GAGGCGAAAG CAGAAAGGGG CTCGGTACCG CACCCAGCCC 
ATCACAGTGG ACGAAGTCAG CCTGCTTCAG AAAGGCCCCA TTCAGCTTCC TGCCTTCAGT 
TTGTCCCCTA ATCTGCACGA CAGACAGCAG GCCTCGCCTG TCAACCTGAA ACCCAGCGAG 
GTGCGCCTTA TACAACCAAT ATCTGAACTT TCAGAACCTG AATCAACGCA CCGGGGCTCG 
CGGGACTGCA ACTCCGTACC GCCGTCGAAG GGAATCCTGA AGAAGAGCTG CCCTGAAGGC 
TCAGAGTGGA GCGGGACCGG CGGTCGGGAG CCGCTACCCA ACCACCAACA AAATGGTAGA 
GACGTCACCG ATGCAGAGAT GTTTGGGAGG ACGGAGGGAG CTGAGAGGCT GGAATCTCAG 
AGGGAGACAC CGGGCGTGCA GCGAAGTTCA GTCCGAGCAG CTCCGTGGAG GCAGAGGGCT 
CGAAACCGGA GGGAGACCCC GACACCGGCC TCCTCAGAGC GGGATGCTCC TCAGGAGGAG 
AGGCCTTCTC GGAGCGGCCC GCAGCAGGAG ATCGCTTCCT CTGTGGGAAA CGGCTCAGAT 
GTGACGAGAA CACAAGATGA AGAGCAGAGC GACAAACCGA AACAAAACGT TTTGGTCTGC 
GGTGGAGTTC GCTCACCTAA ACAGCAGGAA GCAGCAGCAT CTGTGAGAGG CACGGAGCCA 
TCAGAAAGCC CTCCTCTTGA GACACCTCAG TGCTGGACCA GCCTGTGCTT TGAGGCCCAA 
GAAGTCTCCT CTCCTACTAA GAGCGCAGTC CAGCCCCAGT GGAGGCAGAA GGCTAAAGAA 
GCCGAGGATG AGCTTCAGAT GGAAGAAGCT GAAGAGAGGA CCGTGCAGCA GCAAAGCAGC 
GCACAGGAAG CTGCGATCAG CGTCAAGAGA AGTCCGGGAT CTCCGGCTGA ACCTGACAGT 
AGGGATGTTT CCAAGAAGAA GATGGACTCC AGTGAAGCTA CGACAGAGAG TGTCTCACAC 
ATGAACCCGG CTCACGAGGA GGCACCAGTC GATAGTTCCA AGATGTCGGA CGGAGGTTTC 
TGCGGCGGCT GCTTGCCTCC TCCAGCAGGC GGCCCCACAC CGTGCGGCGA CGTGGCTGCG 
TCAGAGAGGG AGCGCGACTT TGCCACCCTC TGCCAAAGCA GCATGCCCAT ACTCAGCTCA 
GCGGTCGCAG AGCACAGACG AGCAGTGCGT CCGTCTCGTC GGACCCAGGG CTCCAGAAAC 
CCCCTTAGGG CTCTGGCAGC CAGAGAGGAC ATCAGGCAGG ACTACATGGG GGACCGGGAC 
AACACCGCAG CCGAGGAGAG AGGCCAAGCA GAAAAAAACT GTAAGAACTC TTCCCCGCCG 
GAGTCACTTC CCATCACCTC TTCAGATAAG GAGACAACCA GGACTTTCCC TCCGTTCAGC 
AACCTGATGC TAATTCACGT CAAGGGGAGG CGGCAGGTCC AGGTACGTCT GGTAGAACCC 
TCGGTCCGTT CGCTGAACAG CGGAGACTGC TTCCTGCTCG TCGCTCCGGA GCATTGCATC 
CTGTGGAGCG GAGAGTTTGC CAACAAAGAG GAGAGGGCAA AGGCCCTTGA ACTGGCAGCC 
TTTATCCAGG GCCATAGGGA CCTCGGCTGT CAGGCCCCCC AGGTGCTCCA CCTGGAGGAA 
GGCTTGAACT CCGATGGCGC CGACGCTGCA GACTTTTGGA GCCTTCTCGG AGGGAGACAA 
CGTTACAGAG GAGCTGGTGC TCCAGAAGAG GATGCGCTGT TTGAGCAAGG AGTGGTGGAG 
TCCAACTGTG TTTACAGCCT AGTGGAGGAC AGATTGGTGC CTCACGAACG GGCCTGGGCC 
TCCGTCCCCA GCACCTCCCT GCTGGGCTCC TCTGAGGTCC TGGTGTTTGA TTTCGGCAGC 
GAGGTGTATC TGTGGCACGG ACGGGATGTT TCACCCGGCA GGAAGGACGT TGCCCTCCAG 
CTGACTCAGC AGGTGTGGGC GGGCGAGTAC GACTACGGCA ACTGTCGAGT CAACCCACTG 
GATCCCACTC AGAGCAACCC CAGCGTTCAG CCGAGGGGAG AAGGCCGTCC CAGGTGGGCT 
CTGCTCGCCG TGGTCTCCGA GGACAGCGAG ACGGCTCTTT TCAGGGAAAA GTTCCAGGGC 
CCGACGCGTG GAAGCGGAGA CACCGTAGAA GTGACTTCAG GGAGAGAAGA GATGCAGGAA 
TATTACAAAC GATTGATAAT TTTCCAGTCC GTGCTTGCTG AACCACCCCA GCCTCAGTCG 
TCATCATCGG ACGGCCTGAG TCCCTGTGAT GTTAAAGCTC TGGCGTCAGG CGGGCTGCTG 
GAGGAGGGCG TTTCCGTGCG GACCATTCTG GCTGGGGTCG ACGTTCGTAG AGGCTGTGGG 
GTCATCACCC TGGAGGAGGG CCATCAGATG GAGCTGAGGA CCGTCTCCGT GGACACCTGG 
CATGTCCAGG AGTTTGACGA CAGTGAAATC CCAGTAGAGA GCACGGGGCA GCTGCACGAA 
GGAGATTCCT ATGTGATCCG CTGGACATAC AGCATCAGTG CTGTTGATAA CCAGGATGAG 
TGTGGCAATG CTTCCAAATC TAAGGAAAAC ACAGCGTTTT TCTTGTGGCG TGGTCGGCAC 
TCCAGCGTCA GCGGAGAAGA CACAGCAGCT TTTCTATCCA TCGGGATGAA CGAGCAGGAA 
GACTCACAGG TGGTCGTACC TCAGGGTGCC GAACCCCCCT GCTTCCTGCA GCTCTTCCAG 
GGAGGCCTGG TCATCCACAA GGGGAAGCGA GAGGAGGCCA CCTCCACTGC AGCAGACTGG 
CGTCTGTTTT GCGTGCGTGG AGAGCTGCCA GAGGAGGCTT TGCTGCTAGA GGGGGAATGC 
TGCTGTTCTA GTCTGAGGTC CAGGGGCTCT GTAGTGCTGC TGAACAGCCA GCAGGGGGCG 
CTGTATCTCT GGACTGGCTG TAAAGCCCAT AGTGGCGCCC GTGAAGTCAG TAAGAGGGCA 
GTGGAGCGTC TCACTGAAAT GAGTCCATTG GAGCTGGGAC TCTGCAAAAA CAACCCTGTG 
AAGGTGCACT TTGTGGAGGA GGGTTCAGAA CCTGCAGACT TCTGGACGGC ACTGGGCCAG 
ATGAACAGGA AGGCCTATGA CTGCATGCTG CAAGATCCGG GAAAGTATAA CTTCACCCCC 
CGCCTCTTCC ACCTGAGTGC CTCTTCCGGT AAATTTAAAG CGGAGGAGCT CCAGAGTTTG 
ATCCGGCTGC CGGGGGTCGT GATGGCCATG CCCTTCGTCC AGGAAAGCTT GTACACTGTT 
CCTCAACCAG CCATGTTCCT GCTTGACAAC CGATTAGAGG TCTACCTGTG GCAGAGAGGT 
CAGCCTGAGC AAACGGAGAG CTCCGCTTCA GCCTGGAGCC TGTGGCATAA CGAGAGAAAG 
TGTGCCATGC AGACGGCTCT GCAGTACTGC AAAGAGATAA ACCCGAGACG GCCCCCGCAG 
GCGTATCTCA TCTTAGAGGG ATCAGAGCCT CTCACCTTCA CTAATGTCTT CCCCCGCTGG 
GAGAGGAACC TCGGACCCAC CACACAGGCA TGCACACACA GTTACATAAT TAGTATTTCC 
TGCCAGAAAG TAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC105916477 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム