AYO20_11644 cDNA ORF clone, Fonsecaea nubica

Gene
Clones
gRNAs

Summary

Gene Symbol	AYO20_11644
Entrez Gene ID	34595020
Full Name	tRNA pseudouridine(38-40) synthase, variant
Gene Type	protein-coding
Organism	Fonsecaea nubica

mRNA and Protein(s)

mRNA	Protein	Name
XM_022649867.1	XP_022494157.1	tRNA pseudouridine(38-40) synthase, variant

Pathways from BioSystems
Gene Ontology

The following AYO20_11644 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AYO20_11644 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFo92620	XM_022649867.1 Latest version!	Fonsecaea nubica tRNA pseudouridine(38-40) synthase, variant (AYO20_11644), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AYO20_11644 ( XM_022649867.1 ) cDNA ORF clone, Fonsecaea nubica -> XP_022494157.1 Fonsecaea nubica tRNA pseudouridine(38-40) synthase, variant (AYO20_11644), partial mRNA.

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CloneID	OFo92620
Clone ID Related Accession (Same CDS sequence)	XM_022649867.1
Accession Version	XM_022649867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Fonsecaea nubica
Product	tRNA pseudouridine(38-40) synthase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_019173848). COMPLETENESS: incomplete on both ends.

ATGGAGACTG GCATTTCGAA GGCCCCGGCG CCAGGTGCGC CCGAAGGTCC CTCTACCACC 
AGCGTCGAGT CAGAGTCGAA GCCTCCAACC TCTCACCAAG GTGGAGCAGC GCATGTTCGT 
GAACAGGAAG GAGGTGGTTC AGATGCAAGA CGTTCGAATC AGAATGGCGC TACAAGGCGA 
GATGCCCGAG GTGGTCGAGG AGGTCGTCAG GGCAAGAAGA GAGATATGGG GAGGAAGGAG 
TGGGCTAAGA CCAAAGTAGA CAAACGCCAA CGGAACGATG ACGAGCAAGC CGCCAAACGC 
CAGCGCATCG ACCAAGGCAA GGAGGAACTA CCGATTTACG CTACGAAGTT CAGCGAGGAA 
GTGCTTGCGG CCGAGGAGAG AAGACCTAAA AAGAAGGTTG CTGTCCTTAT TGGATACGCT 
GGAACTGGCT ACAAGGGGAT GCAATTAGCG CATGACCAGA AAACGATCGA AGGAGACCTC 
TTCCAAGCCT TCGTTGCGGC CGGCGCCATA TCCAAAGCCA ACGCTGACGA TCCCAAGAAG 
TCTTCCTTTG TGCGGTGCGC GCGGACCGAC AAGGGTGTCC ATGCCGCCGG CAACGTGATA 
TCACTGAAGT TGATCATCGA AGACGAAGAC ATAGTGAAGA AGATCAATGA AAACCTGGTC 
CCCCAAATTC GGGTCTGGGG GTATGAACGG ACCAGTAACA GTTTCAGTGC TTATCAATTG 
GTCGACTCTC GGATATATGA GTATCTCATT CCTACACACA GCTTCCTACC ACCACACCCG 
AGCAGTTTTC TTGCGAGGAA CTGTGAAAAA TGGGCAGAGA AGAAGGGTGA CGTGGACGGG 
TACAGAAAGA GACAGGAAGA AGTATTGGGA TACTGGGAAC GGGTCGACGA GGAGATGATC 
AAGCCGATTC TGGCGGAATA CGACGAAGAG ATTCGTGCCA TTCTAGAGAA GGCACTGTGG 
CTGAAAACAG AAGGTGACTA CGACGGGGCC AGTGGTGCAG ATCTGTCGAT TCCCACGACT 
ACGGAGGTTG AAGGAGACGT TTCCAAACCA GAGTTGCCGC ATGTAGCGGA AGGCAAAGGT 
GCAGAATCGA AGGGCAAGCT TCCTGTAGAC AACAACAACA ACAACAACAG CGAAGAAGAC 
ATCTCCCCAG CAGAAGACTC AACAGATCCA TCCAAATTAC CGCGATCGCG TATCCTCGAA 
GAAGCCACGA AACGGATCCG GCAGGCCTAT ATCGCCGCCA AACGAGCCTA CCGCATTGCC 
CCTGAACGAC TCGACCGGAT CCAAGAGGCA CTCCATCTGT TTGTGGGCAC ACGCAACTTT 
CACAACTACA CCGTCCAGAA GACTTTCAAG GATCCCAGCG CAAAGCGAGT CATCAAATCC 
TTCCTTGTCA ATAGGACGCC CATCCTTATC AACGGAACAG AGTGGCTGAG TATGAAAGTG 
CACGGGCAGA GTTTTATGAT GCACCAGATC CGCAAGATGG TCGGGATGGT CGCGCTCGTC 
GTGCGTTGTG GATGTGAGCC TCAGCGACTA GTAGAGAGCA TGGGACCTGA CAGACTGTCC 
ATCCCCAAAG CTCCCAGCTT GGGTCTTCTG CTCGAACGTC CGGTATTCGA ATCATACAAC 
AAGCGTGCCA AAGACTCACT CGCCAAAGAA GCCATCGACT TTGACAAATA CAAGGAGGAG 
ATGGACAAGT TCAAGCAGGA ACAAATTTAC GAGCGCATGT ACCGGGACGA GGAGAAGGAC 
AACGTCTTTG GCAATTTCTT CAATCACGTC GACAACTTTC CTAGCGAACC CTTTTTGTTT 
GTCACCTCAG GCGGCATCGA AGCAACAAGG ACGAAACCGT TGGTTGGAGC GATTGAGAAA 
GACGAGACGA AGGGTGCGGA CGCTCTATTG AATGATGGGG ATGTCGGTGT CGACGACGAG 
GACAGCGACG CGGACAATAC TGTCAAGGAT AACCATGGTG ACGAAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022494157.1
CDS	1..1971
Translation	METGISKAPAPGAPEGPSTTSVESESKPPTSHQGGAAHVREQEGGGSDARRSNQNGATRRDARGGRGGRQGKKRDMGRKEWAKTKVDKRQRNDDEQAAKRQRIDQGKEELPIYATKFSEEVLAAEERRPKKKVAVLIGYAGTGYKGMQLAHDQKTIEGDLFQAFVAAGAISKANADDPKKSSFVRCARTDKGVHAAGNVISLKLIIEDEDIVKKINENLVPQIRVWGYERTSNSFSAYQLVDSRIYEYLIPTHSFLPPHPSSFLARNCEKWAEKKGDVDGYRKRQEEVLGYWERVDEEMIKPILAEYDEEIRAILEKALWLKTEGDYDGASGADLSIPTTTEVEGDVSKPELPHVAEGKGAESKGKLPVDNNNNNNSEEDISPAEDSTDPSKLPRSRILEEATKRIRQAYIAAKRAYRIAPERLDRIQEALHLFVGTRNFHNYTVQKTFKDPSAKRVIKSFLVNRTPILINGTEWLSMKVHGQSFMMHQIRKMVGMVALVVRCGCEPQRLVESMGPDRLSIPKAPSLGLLLERPVFESYNKRAKDSLAKEAIDFDKYKEEMDKFKQEQIYERMYRDEEKDNVFGNFFNHVDNFPSEPFLFVTSGGIEATRTKPLVGAIEKDETKGADALLNDGDVGVDDEDSDADNTVKDNHGDEG

Target ORF information:

RefSeq Version	XM_022649867.1
Organism	Fonsecaea nubica
Definition	Fonsecaea nubica tRNA pseudouridine(38-40) synthase, variant (AYO20_11644), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022649867.1

ORF Insert Sequence:

ATGGAGACTG GCATTTCGAA GGCCCCGGCG CCAGGTGCGC CCGAAGGTCC CTCTACCACC 
AGCGTCGAGT CAGAGTCGAA GCCTCCAACC TCTCACCAAG GTGGAGCAGC GCATGTTCGT 
GAACAGGAAG GAGGTGGTTC AGATGCAAGA CGTTCGAATC AGAATGGCGC TACAAGGCGA 
GATGCCCGAG GTGGTCGAGG AGGTCGTCAG GGCAAGAAGA GAGATATGGG GAGGAAGGAG 
TGGGCTAAGA CCAAAGTAGA CAAACGCCAA CGGAACGATG ACGAGCAAGC CGCCAAACGC 
CAGCGCATCG ACCAAGGCAA GGAGGAACTA CCGATTTACG CTACGAAGTT CAGCGAGGAA 
GTGCTTGCGG CCGAGGAGAG AAGACCTAAA AAGAAGGTTG CTGTCCTTAT TGGATACGCT 
GGAACTGGCT ACAAGGGGAT GCAATTAGCG CATGACCAGA AAACGATCGA AGGAGACCTC 
TTCCAAGCCT TCGTTGCGGC CGGCGCCATA TCCAAAGCCA ACGCTGACGA TCCCAAGAAG 
TCTTCCTTTG TGCGGTGCGC GCGGACCGAC AAGGGTGTCC ATGCCGCCGG CAACGTGATA 
TCACTGAAGT TGATCATCGA AGACGAAGAC ATAGTGAAGA AGATCAATGA AAACCTGGTC 
CCCCAAATTC GGGTCTGGGG GTATGAACGG ACCAGTAACA GTTTCAGTGC TTATCAATTG 
GTCGACTCTC GGATATATGA GTATCTCATT CCTACACACA GCTTCCTACC ACCACACCCG 
AGCAGTTTTC TTGCGAGGAA CTGTGAAAAA TGGGCAGAGA AGAAGGGTGA CGTGGACGGG 
TACAGAAAGA GACAGGAAGA AGTATTGGGA TACTGGGAAC GGGTCGACGA GGAGATGATC 
AAGCCGATTC TGGCGGAATA CGACGAAGAG ATTCGTGCCA TTCTAGAGAA GGCACTGTGG 
CTGAAAACAG AAGGTGACTA CGACGGGGCC AGTGGTGCAG ATCTGTCGAT TCCCACGACT 
ACGGAGGTTG AAGGAGACGT TTCCAAACCA GAGTTGCCGC ATGTAGCGGA AGGCAAAGGT 
GCAGAATCGA AGGGCAAGCT TCCTGTAGAC AACAACAACA ACAACAACAG CGAAGAAGAC 
ATCTCCCCAG CAGAAGACTC AACAGATCCA TCCAAATTAC CGCGATCGCG TATCCTCGAA 
GAAGCCACGA AACGGATCCG GCAGGCCTAT ATCGCCGCCA AACGAGCCTA CCGCATTGCC 
CCTGAACGAC TCGACCGGAT CCAAGAGGCA CTCCATCTGT TTGTGGGCAC ACGCAACTTT 
CACAACTACA CCGTCCAGAA GACTTTCAAG GATCCCAGCG CAAAGCGAGT CATCAAATCC 
TTCCTTGTCA ATAGGACGCC CATCCTTATC AACGGAACAG AGTGGCTGAG TATGAAAGTG 
CACGGGCAGA GTTTTATGAT GCACCAGATC CGCAAGATGG TCGGGATGGT CGCGCTCGTC 
GTGCGTTGTG GATGTGAGCC TCAGCGACTA GTAGAGAGCA TGGGACCTGA CAGACTGTCC 
ATCCCCAAAG CTCCCAGCTT GGGTCTTCTG CTCGAACGTC CGGTATTCGA ATCATACAAC 
AAGCGTGCCA AAGACTCACT CGCCAAAGAA GCCATCGACT TTGACAAATA CAAGGAGGAG 
ATGGACAAGT TCAAGCAGGA ACAAATTTAC GAGCGCATGT ACCGGGACGA GGAGAAGGAC 
AACGTCTTTG GCAATTTCTT CAATCACGTC GACAACTTTC CTAGCGAACC CTTTTTGTTT 
GTCACCTCAG GCGGCATCGA AGCAACAAGG ACGAAACCGT TGGTTGGAGC GATTGAGAAA 
GACGAGACGA AGGGTGCGGA CGCTCTATTG AATGATGGGG ATGTCGGTGT CGACGACGAG 
GACAGCGACG CGGACAATAC TGTCAAGGAT AACCATGGTG ACGAAGGCTG A