PFKM cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKM
Entrez Gene ID	100034116
Full Name	phosphofructokinase, muscle
Synonyms	ATP-PFK,PFK-A,PFK-M
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005611076.2	XP_005611133.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
XM_023642167.1	XP_023497935.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
XM_014740748.1	XP_014596234.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_005611076.3	XP_005611133.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
NM_001081922.2	NP_001075391.1	ATP-dependent 6-phosphofructokinase, muscle type

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando L, MacLeod JN
PloS one10(6)e0124375(2015)

Molecular characterization of glycogen synthase 1 and its tissue expression profile with type II hexokinase and muscle-type phosphofructokinase in horses.
Echigoya Y, Okabe H, Itou T, Endo H, Sakai T
Molecular biology reports38(1)461-9(2011 Jan)

Molecular cloning of equine muscle-type phosphofructokinase cDNA.
Sato T, Itou T, Sakai T
The Journal of veterinary medical science65(5)645-8(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The tissue expression profiles of eGYS1, equine type II hexokinase (eHKII) and muscle-type phosphofructokinase (ePFKM) were determined by real-time PCR and western blot analysis.
Title: Molecular characterization of glycogen synthase 1 and its tissue expression profile with type II hexokinase and muscle-type phosphofructokinase in horses.

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb00123	NM_001081922.2 Latest version!	Equus caballus phosphofructokinase, muscle (PFKM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OHb22366	XM_005611076.3 Latest version!	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OHb31354	XM_014740748.1 Latest version!	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OHb66016	XM_023642167.1 Latest version!	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHb22366	XM_005611076.2	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKM ( NM_001081922.2 ) cDNA ORF clone, Equus caballus(horse) -> NP_001075391.1 Equus caballus phosphofructokinase, muscle (PFKM), mRNA.

Price & Availability↑

CloneID	OHb00123
Clone ID Related Accession (Same CDS sequence)	NM_001081922.2
Accession Version	NM_001081922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Equus caballus(horse)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB088683.1 and CX601578.1. On May 14, 2015 this sequence version replaced NM_001081922.1. ##Evidence-Data-START## Transcript exon combination :: AB088683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2452530, SAMEA2452531 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGACC ACATCAGGGA GGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAGGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCTTGGGATT ACCAACCTGT GTGTCATTGG GGGCGATGGT 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC TGAGGAGGCT ACGAAGTCCA ACTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATTGACAA TGACTTTTGC GGCACTGATA TGACCATTGG CACTGACTCT 
GCCCTGCACC GGATCATAGA GATTGTAGAC GCCATCACTA CTACTGCTCA GAGCCACCAG 
AGGACGTTCG TGTTAGAGGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA TGACTGGGAG 
GATCATCTTT GTCGTCGGCT CAGTGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAGGAAT GGGAAACCAA TCACCTCAGA ATCCATCAAG 
GATCTGGTGG TAAAGCGTCT GGGATATGAC ACCCGGGTCA CCGTCTTGGG GCATGTGCAG 
CGAGGTGGGA CCCCGTCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGC GACCCCAGAC ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT 
AATCAGGCTG TGCGGCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGCTG AGAGGAAATT TGATGAGGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTGGCTCAC ATCAGACCCC CAGTTTCTAA GAGTGGCTCA 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCGGCTGCAG GCATGAATGC TGCCGTCCGC 
TCCACTGTGA GAATTGGCCT CATCCAGGGC AACCGAGTGC TGGTTGTGCA CGATGGCTTC 
GAGGGCCTGG CCAAGGGCAG AATTGAGGAA GCTGGCTGGA GCTACGTTGG CGGCTGGACT 
GGCCAAGGGG GTTCTAAACT TGGGACTAAA AGGACGCTAC CCAGGAAGAG CTTCGAGCAG 
ATCAGTGCCA ACATCACTAA GTTCAACATT CAAGGCCTGA TCATCGTTGG GGGCTTCGAG 
GCTTACACAG GGGGCCTGGA GCTGATGGAG GGCAGGAAGC AGTACGACGA GCTCTGCATC 
CCGTTTGTGG TCATCCCTGC CACGGTCTCC AACAACGTCC CTGGCTCAGA CTTCAGCGTG 
GGGGCTGACA CCGCACTCAA TACTATCTGC ATGACCTGTG ACCGCATCAA GCAGTCAGCA 
GCAGGTACCA AGCGTCGAGT GTTTATCATT GAAACCATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CAGGACTGGC AGCTGGAGCC GATGCGGCCT ACATTTTCGA GGAGCCATTC 
ACCATTCGAG ACCTGCAGGT GAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAGAAG TGCAATGAGA ACTATAGCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGC 
CACATGCAGC AGGGTGGGAG CCCAACTCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGT 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CCCCAGACTC AGGCTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAACCAGTGA CTGAGCTGAA GGAACAGACG GATTTCGAGC ACCGCATCCC CAAGGAACAG 
TGGTGGCTGA AGCTGAGGCC CATCCTCAAG ATCCTAGCCA AGTATGAGAT TGACCTGGAT 
ACATCAGAGC ACGCCCACCT GGAGCACATC AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075391.1
CDS	1..2343
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKLGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSNYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEDHLCRRLSETRTRGSRLNIIIVAEGAIDRNGKPITSESIKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMAERKFDEAMKLRGRSFMNNWEVYKLLAHIRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGRIEEAGWSYVGGWTGQGGSKLGTKRTLPRKSFEQISANITKFNIQGLIIVGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYSTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEAPA

Target ORF information:

RefSeq Version	NM_001081922.2
Organism	Equus caballus(horse)
Definition	Equus caballus phosphofructokinase, muscle (PFKM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081922.2

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGACC ACATCAGGGA GGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAGGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCTTGGGATT ACCAACCTGT GTGTCATTGG GGGCGATGGT 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC TGAGGAGGCT ACGAAGTCCA ACTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATTGACAA TGACTTTTGC GGCACTGATA TGACCATTGG CACTGACTCT 
GCCCTGCACC GGATCATAGA GATTGTAGAC GCCATCACTA CTACTGCTCA GAGCCACCAG 
AGGACGTTCG TGTTAGAGGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA TGACTGGGAG 
GATCATCTTT GTCGTCGGCT CAGTGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAGGAAT GGGAAACCAA TCACCTCAGA ATCCATCAAG 
GATCTGGTGG TAAAGCGTCT GGGATATGAC ACCCGGGTCA CCGTCTTGGG GCATGTGCAG 
CGAGGTGGGA CCCCGTCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGC GACCCCAGAC ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT 
AATCAGGCTG TGCGGCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGCTG AGAGGAAATT TGATGAGGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTGGCTCAC ATCAGACCCC CAGTTTCTAA GAGTGGCTCA 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCGGCTGCAG GCATGAATGC TGCCGTCCGC 
TCCACTGTGA GAATTGGCCT CATCCAGGGC AACCGAGTGC TGGTTGTGCA CGATGGCTTC 
GAGGGCCTGG CCAAGGGCAG AATTGAGGAA GCTGGCTGGA GCTACGTTGG CGGCTGGACT 
GGCCAAGGGG GTTCTAAACT TGGGACTAAA AGGACGCTAC CCAGGAAGAG CTTCGAGCAG 
ATCAGTGCCA ACATCACTAA GTTCAACATT CAAGGCCTGA TCATCGTTGG GGGCTTCGAG 
GCTTACACAG GGGGCCTGGA GCTGATGGAG GGCAGGAAGC AGTACGACGA GCTCTGCATC 
CCGTTTGTGG TCATCCCTGC CACGGTCTCC AACAACGTCC CTGGCTCAGA CTTCAGCGTG 
GGGGCTGACA CCGCACTCAA TACTATCTGC ATGACCTGTG ACCGCATCAA GCAGTCAGCA 
GCAGGTACCA AGCGTCGAGT GTTTATCATT GAAACCATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CAGGACTGGC AGCTGGAGCC GATGCGGCCT ACATTTTCGA GGAGCCATTC 
ACCATTCGAG ACCTGCAGGT GAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAGAAG TGCAATGAGA ACTATAGCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGC 
CACATGCAGC AGGGTGGGAG CCCAACTCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGT 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CCCCAGACTC AGGCTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAACCAGTGA CTGAGCTGAA GGAACAGACG GATTTCGAGC ACCGCATCCC CAAGGAACAG 
TGGTGGCTGA AGCTGAGGCC CATCCTCAAG ATCCTAGCCA AGTATGAGAT TGACCTGGAT 
ACATCAGAGC ACGCCCACCT GGAGCACATC AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005611076.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005611133.1 Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb22366
Clone ID Related Accession (Same CDS sequence)	XM_005611076.2 , XM_005611076.3
Accession Version	XM_005611076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005611076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGACC ACATCAGGGA GGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAGGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCTTGGGATT ACCAACCTGT GTGTCATTGG GGGCGATGGT 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC TGAGGAGGCT ACGAAGTCCA ACTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATTGACAA TGACTTTTGC GGCACTGATA TGACCATTGG CACTGACTCT 
GCCCTGCACC GGATCATAGA GATTGTAGAC GCCATCACTA CTACTGCTCA GAGCCACCAG 
AGGACGTTCG TGTTAGAGGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA TGACTGGGAG 
GATCATCTTT GTCGTCGGCT CAGTGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAGGAAT GGGAAACCAA TCACCTCAGA ATCCATCAAG 
GATCTGGTGG TAAAGCGTCT GGGATATGAC ACCCGGGTCA CCGTCTTGGG GCATGTGCAG 
CGAGGTGGGA CCCCGTCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGC GACCCCAGAC ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT 
AATCAGGCTG TGCGGCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGCTG AGAGGAAATT TGATGAGGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTGGCTCAC ATCAGACCCC CAGTTTCTAA GAGTGGCTCA 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCGGCTGCAG GCATGAATGC TGCCGTCCGC 
TCCACTGTGA GAATTGGCCT CATCCAGGGC AACCGAGTGC TGGTTGTGCA CGATGGCTTC 
GAGGGCCTGG CCAAGGGCCA GATTGAGGAA GCTGGCTGGA GCTACGTTGG CGGCTGGACT 
GGCCAAGGGG GTTCTAAACT TGGGACTAAA AGGACGCTAC CCAGGAAGAG CTTCGAGCAG 
ATCAGTGCCA ACATCACTAA GTTCAACATT CAAGGCCTGA TCATCGTTGG GGGCTTCGAG 
GCTTACACAG GGGGCCTGGA GCTGATGGAG GGCAGGAAGC AGTACGACGA GCTCTGCATC 
CCGTTTGTGG TCATCCCTGC CACGGTCTCC AACAACGTCC CTGGCTCAGA CTTCAGCGTG 
GGGGCTGACA CCGCACTCAA TACTATCTGC ATGACCTGTG ACCGCATCAA GCAGTCAGCA 
GCAGGTACCA AGCGTCGAGT GTTTATCATT GAAACCATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CAGGACTGGC AGCTGGAGCC GATGCGGCCT ACATTTTCGA GGAGCCATTC 
ACCATTCGAG ACCTGCAGGT GAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAGAAG TGCAATGAGA ACTATAGCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGC 
CACATGCAGC AGGGTGGGAG CCCAACTCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGT 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CCCCAGACTC AGGCTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAACCAGTGA CTGAGCTGAA GGAACAGACG GATTTCGAGC ACCGCATCCC CAAGGAACAG 
TGGTGGCTGA AGCTGAGGCC CATCCTCAAG ATCCTAGCCA AGTATGAGAT TGACCTGGAT 
ACATCAGAGC ACGCCCACCT GGAGCACATC AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005611133.1
CDS	97..2439
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKLGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSNYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEDHLCRRLSETRTRGSRLNIIIVAEGAIDRNGKPITSESIKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMAERKFDEAMKLRGRSFMNNWEVYKLLAHIRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPRKSFEQISANITKFNIQGLIIVGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYSTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEAPA

Target ORF information:

RefSeq Version	XM_005611076.2
Organism	Equus caballus(horse)
Definition	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005611076.2

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGACC ACATCAGGGA GGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAGGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCTTGGGATT ACCAACCTGT GTGTCATTGG GGGCGATGGT 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC TGAGGAGGCT ACGAAGTCCA ACTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATTGACAA TGACTTTTGC GGCACTGATA TGACCATTGG CACTGACTCT 
GCCCTGCACC GGATCATAGA GATTGTAGAC GCCATCACTA CTACTGCTCA GAGCCACCAG 
AGGACGTTCG TGTTAGAGGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA TGACTGGGAG 
GATCATCTTT GTCGTCGGCT CAGTGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAGGAAT GGGAAACCAA TCACCTCAGA ATCCATCAAG 
GATCTGGTGG TAAAGCGTCT GGGATATGAC ACCCGGGTCA CCGTCTTGGG GCATGTGCAG 
CGAGGTGGGA CCCCGTCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGC GACCCCAGAC ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT 
AATCAGGCTG TGCGGCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGCTG AGAGGAAATT TGATGAGGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTGGCTCAC ATCAGACCCC CAGTTTCTAA GAGTGGCTCA 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCGGCTGCAG GCATGAATGC TGCCGTCCGC 
TCCACTGTGA GAATTGGCCT CATCCAGGGC AACCGAGTGC TGGTTGTGCA CGATGGCTTC 
GAGGGCCTGG CCAAGGGCCA GATTGAGGAA GCTGGCTGGA GCTACGTTGG CGGCTGGACT 
GGCCAAGGGG GTTCTAAACT TGGGACTAAA AGGACGCTAC CCAGGAAGAG CTTCGAGCAG 
ATCAGTGCCA ACATCACTAA GTTCAACATT CAAGGCCTGA TCATCGTTGG GGGCTTCGAG 
GCTTACACAG GGGGCCTGGA GCTGATGGAG GGCAGGAAGC AGTACGACGA GCTCTGCATC 
CCGTTTGTGG TCATCCCTGC CACGGTCTCC AACAACGTCC CTGGCTCAGA CTTCAGCGTG 
GGGGCTGACA CCGCACTCAA TACTATCTGC ATGACCTGTG ACCGCATCAA GCAGTCAGCA 
GCAGGTACCA AGCGTCGAGT GTTTATCATT GAAACCATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CAGGACTGGC AGCTGGAGCC GATGCGGCCT ACATTTTCGA GGAGCCATTC 
ACCATTCGAG ACCTGCAGGT GAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAGAAG TGCAATGAGA ACTATAGCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGC 
CACATGCAGC AGGGTGGGAG CCCAACTCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGT 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CCCCAGACTC AGGCTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAACCAGTGA CTGAGCTGAA GGAACAGACG GATTTCGAGC ACCGCATCCC CAAGGAACAG 
TGGTGGCTGA AGCTGAGGCC CATCCTCAAG ATCCTAGCCA AGTATGAGAT TGACCTGGAT 
ACATCAGAGC ACGCCCACCT GGAGCACATC AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005611076.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005611133.1 Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb22366
Clone ID Related Accession (Same CDS sequence)	XM_005611076.2 , XM_005611076.3
Accession Version	XM_005611076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005611076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGACC ACATCAGGGA GGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAGGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCTTGGGATT ACCAACCTGT GTGTCATTGG GGGCGATGGT 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC TGAGGAGGCT ACGAAGTCCA ACTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATTGACAA TGACTTTTGC GGCACTGATA TGACCATTGG CACTGACTCT 
GCCCTGCACC GGATCATAGA GATTGTAGAC GCCATCACTA CTACTGCTCA GAGCCACCAG 
AGGACGTTCG TGTTAGAGGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA TGACTGGGAG 
GATCATCTTT GTCGTCGGCT CAGTGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAGGAAT GGGAAACCAA TCACCTCAGA ATCCATCAAG 
GATCTGGTGG TAAAGCGTCT GGGATATGAC ACCCGGGTCA CCGTCTTGGG GCATGTGCAG 
CGAGGTGGGA CCCCGTCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGC GACCCCAGAC ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT 
AATCAGGCTG TGCGGCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGCTG AGAGGAAATT TGATGAGGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTGGCTCAC ATCAGACCCC CAGTTTCTAA GAGTGGCTCA 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCGGCTGCAG GCATGAATGC TGCCGTCCGC 
TCCACTGTGA GAATTGGCCT CATCCAGGGC AACCGAGTGC TGGTTGTGCA CGATGGCTTC 
GAGGGCCTGG CCAAGGGCCA GATTGAGGAA GCTGGCTGGA GCTACGTTGG CGGCTGGACT 
GGCCAAGGGG GTTCTAAACT TGGGACTAAA AGGACGCTAC CCAGGAAGAG CTTCGAGCAG 
ATCAGTGCCA ACATCACTAA GTTCAACATT CAAGGCCTGA TCATCGTTGG GGGCTTCGAG 
GCTTACACAG GGGGCCTGGA GCTGATGGAG GGCAGGAAGC AGTACGACGA GCTCTGCATC 
CCGTTTGTGG TCATCCCTGC CACGGTCTCC AACAACGTCC CTGGCTCAGA CTTCAGCGTG 
GGGGCTGACA CCGCACTCAA TACTATCTGC ATGACCTGTG ACCGCATCAA GCAGTCAGCA 
GCAGGTACCA AGCGTCGAGT GTTTATCATT GAAACCATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CAGGACTGGC AGCTGGAGCC GATGCGGCCT ACATTTTCGA GGAGCCATTC 
ACCATTCGAG ACCTGCAGGT GAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAGAAG TGCAATGAGA ACTATAGCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGC 
CACATGCAGC AGGGTGGGAG CCCAACTCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGT 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CCCCAGACTC AGGCTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAACCAGTGA CTGAGCTGAA GGAACAGACG GATTTCGAGC ACCGCATCCC CAAGGAACAG 
TGGTGGCTGA AGCTGAGGCC CATCCTCAAG ATCCTAGCCA AGTATGAGAT TGACCTGGAT 
ACATCAGAGC ACGCCCACCT GGAGCACATC AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005611133.1
CDS	143..2485
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKLGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSNYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEDHLCRRLSETRTRGSRLNIIIVAEGAIDRNGKPITSESIKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMAERKFDEAMKLRGRSFMNNWEVYKLLAHIRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPRKSFEQISANITKFNIQGLIIVGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYSTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEAPA

Target ORF information:

RefSeq Version	XM_005611076.3
Organism	Equus caballus(horse)
Definition	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005611076.3

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TCGGCAAAGC CATCGCCGTG 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CTGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGACC ACATCAGGGA GGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGT 
GGCACAGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAGGG ACGACTCCGA 
GCTGCCCACA ACCTGGTGAA GCTTGGGATT ACCAACCTGT GTGTCATTGG GGGCGATGGT 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCGGGTA AGATCACAGC TGAGGAGGCT ACGAAGTCCA ACTACCTGAA CATCGTGGGC 
CTGGTTGGCT CAATTGACAA TGACTTTTGC GGCACTGATA TGACCATTGG CACTGACTCT 
GCCCTGCACC GGATCATAGA GATTGTAGAC GCCATCACTA CTACTGCTCA GAGCCACCAG 
AGGACGTTCG TGTTAGAGGT GATGGGCCGG CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTCTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA TGACTGGGAG 
GATCATCTTT GTCGTCGGCT CAGTGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCGAT TGACAGGAAT GGGAAACCAA TCACCTCAGA ATCCATCAAG 
GATCTGGTGG TAAAGCGTCT GGGATATGAC ACCCGGGTCA CCGTCTTGGG GCATGTGCAG 
CGAGGTGGGA CCCCGTCAGC CTTTGACCGG ATCCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGC GACCCCAGAC ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT 
AATCAGGCTG TGCGGCTGCC CCTCATGGAG TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGCTG AGAGGAAATT TGATGAGGCC ATGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TATACAAGCT TCTGGCTCAC ATCAGACCCC CAGTTTCTAA GAGTGGCTCA 
CACACAGTGG CCGTGATGAA TGTGGGGGCC CCGGCTGCAG GCATGAATGC TGCCGTCCGC 
TCCACTGTGA GAATTGGCCT CATCCAGGGC AACCGAGTGC TGGTTGTGCA CGATGGCTTC 
GAGGGCCTGG CCAAGGGCCA GATTGAGGAA GCTGGCTGGA GCTACGTTGG CGGCTGGACT 
GGCCAAGGGG GTTCTAAACT TGGGACTAAA AGGACGCTAC CCAGGAAGAG CTTCGAGCAG 
ATCAGTGCCA ACATCACTAA GTTCAACATT CAAGGCCTGA TCATCGTTGG GGGCTTCGAG 
GCTTACACAG GGGGCCTGGA GCTGATGGAG GGCAGGAAGC AGTACGACGA GCTCTGCATC 
CCGTTTGTGG TCATCCCTGC CACGGTCTCC AACAACGTCC CTGGCTCAGA CTTCAGCGTG 
GGGGCTGACA CCGCACTCAA TACTATCTGC ATGACCTGTG ACCGCATCAA GCAGTCAGCA 
GCAGGTACCA AGCGTCGAGT GTTTATCATT GAAACCATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CAGGACTGGC AGCTGGAGCC GATGCGGCCT ACATTTTCGA GGAGCCATTC 
ACCATTCGAG ACCTGCAGGT GAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAGAAG TGCAATGAGA ACTATAGCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGC 
CACATGCAGC AGGGTGGGAG CCCAACTCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGT 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CCCCAGACTC AGGCTGTGTT CTGGGGATGC GTAAGAGGGC CCTGGTCTTT 
CAACCAGTGA CTGAGCTGAA GGAACAGACG GATTTCGAGC ACCGCATCCC CAAGGAACAG 
TGGTGGCTGA AGCTGAGGCC CATCCTCAAG ATCCTAGCCA AGTATGAGAT TGACCTGGAT 
ACATCAGAGC ACGCCCACCT GGAGCACATC AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_014740748.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014596234.1 Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb31354
Clone ID Related Accession (Same CDS sequence)	XM_014740748.1
Accession Version	XM_014740748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTAAAG AGGAATTACA TCTGAAATTT TTCATGTGTG TGATTCAATC TCGCCAATTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCAACTT CCAGCATGCT CATACAAAAG 
CCACCACCTG AGACAGACGC CTCGAAGATT CTAGATACTA TGGATGCTCC AGACACCATG 
GAAGGCATAC ATGTTTTGAA AAATGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATCGGCAA AGCCATCGCC GTGTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACTGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ACCACATCAG GGAGGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGTGGCACAG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA GGGACGACTC CGAGCTGCCC ACAACCTGGT GAAGCTTGGG 
ATTACCAACC TGTGTGTCAT TGGGGGCGAT GGTAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCGG GTAAGATCAC AGCTGAGGAG 
GCTACGAAGT CCAACTACCT GAACATCGTG GGCCTGGTTG GCTCAATTGA CAATGACTTT 
TGCGGCACTG ATATGACCAT TGGCACTGAC TCTGCCCTGC ACCGGATCAT AGAGATTGTA 
GACGCCATCA CTACTACTGC TCAGAGCCAC CAGAGGACGT TCGTGTTAGA GGTGATGGGC 
CGGCACTGTG GATACCTGGC CCTTGTCACC TCTCTCTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGATGACTGG GAGGATCATC TTTGTCGTCG GCTCAGTGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC GATTGACAGG 
AATGGGAAAC CAATCACCTC AGAATCCATC AAGGATCTGG TGGTAAAGCG TCTGGGATAT 
GACACCCGGG TCACCGTCTT GGGGCATGTG CAGCGAGGTG GGACCCCGTC AGCCTTTGAC 
CGGATCCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGCGACCCCA 
GACACCCCAG CCTGCGTGGT GAGCCTCTCT GGTAATCAGG CTGTGCGGCT GCCCCTCATG 
GAGTGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG CTGAGAGGAA ATTTGATGAG 
GCCATGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTATACAA GCTTCTGGCT 
CACATCAGAC CCCCAGTTTC TAAGAGTGGC TCACACACAG TGGCCGTGAT GAATGTGGGG 
GCCCCGGCTG CAGGCATGAA TGCTGCCGTC CGCTCCACTG TGAGAATTGG CCTCATCCAG 
GGCAACCGAG TGCTGGTTGT GCACGATGGC TTCGAGGGCC TGGCCAAGGG CCAGATTGAG 
GAAGCTGGCT GGAGCTACGT TGGCGGCTGG ACTGGCCAAG GGGGTTCTAA ACTTGGGACT 
AAAAGGACGC TACCCAGGAA GAGCTTCGAG CAGATCAGTG CCAACATCAC TAAGTTCAAC 
ATTCAAGGCC TGATCATCGT TGGGGGCTTC GAGGCTTACA CAGGGGGCCT GGAGCTGATG 
GAGGGCAGGA AGCAGTACGA CGAGCTCTGC ATCCCGTTTG TGGTCATCCC TGCCACGGTC 
TCCAACAACG TCCCTGGCTC AGACTTCAGC GTGGGGGCTG ACACCGCACT CAATACTATC 
TGCATGACCT GTGACCGCAT CAAGCAGTCA GCAGCAGGTA CCAAGCGTCG AGTGTTTATC 
ATTGAAACCA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCAGGACT GGCAGCTGGA 
GCCGATGCGG CCTACATTTT CGAGGAGCCA TTCACCATTC GAGACCTGCA GGTGAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAG 
AAGTGCAATG AGAACTATAG CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGCCACATGC AGCAGGGTGG GAGCCCAACT 
CCATTTGATA GGAATTTTGC CACTAAGATG GGTGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACCCCAGA CTCAGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCCCTGGTC TTTCAACCAG TGACTGAGCT GAAGGAACAG 
ACGGATTTCG AGCACCGCAT CCCCAAGGAA CAGTGGTGGC TGAAGCTGAG GCCCATCCTC 
AAGATCCTAG CCAAGTATGA GATTGACCTG GATACATCAG AGCACGCCCA CCTGGAGCAC 
ATCAGTCGGA AGCGGTCTGG AGAAGCTCCT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014596234.1
CDS	10..2565
Translation	MRKEELHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIQKPPPETDASKILDTMDAPDTMEGIHVLKNEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKLGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSNYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEDHLCRRLSETRTRGSRLNIIIVAEGAIDRNGKPITSESIKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMAERKFDEAMKLRGRSFMNNWEVYKLLAHIRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPRKSFEQISANITKFNIQGLIIVGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYSTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEAPA

Target ORF information:

RefSeq Version	XM_014740748.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014740748.1

ORF Insert Sequence:

ATGCGTAAAG AGGAATTACA TCTGAAATTT TTCATGTGTG TGATTCAATC TCGCCAATTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCAACTT CCAGCATGCT CATACAAAAG 
CCACCACCTG AGACAGACGC CTCGAAGATT CTAGATACTA TGGATGCTCC AGACACCATG 
GAAGGCATAC ATGTTTTGAA AAATGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATCGGCAA AGCCATCGCC GTGTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACTGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ACCACATCAG GGAGGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGTGGCACAG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA GGGACGACTC CGAGCTGCCC ACAACCTGGT GAAGCTTGGG 
ATTACCAACC TGTGTGTCAT TGGGGGCGAT GGTAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCGG GTAAGATCAC AGCTGAGGAG 
GCTACGAAGT CCAACTACCT GAACATCGTG GGCCTGGTTG GCTCAATTGA CAATGACTTT 
TGCGGCACTG ATATGACCAT TGGCACTGAC TCTGCCCTGC ACCGGATCAT AGAGATTGTA 
GACGCCATCA CTACTACTGC TCAGAGCCAC CAGAGGACGT TCGTGTTAGA GGTGATGGGC 
CGGCACTGTG GATACCTGGC CCTTGTCACC TCTCTCTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGATGACTGG GAGGATCATC TTTGTCGTCG GCTCAGTGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC GATTGACAGG 
AATGGGAAAC CAATCACCTC AGAATCCATC AAGGATCTGG TGGTAAAGCG TCTGGGATAT 
GACACCCGGG TCACCGTCTT GGGGCATGTG CAGCGAGGTG GGACCCCGTC AGCCTTTGAC 
CGGATCCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGCGACCCCA 
GACACCCCAG CCTGCGTGGT GAGCCTCTCT GGTAATCAGG CTGTGCGGCT GCCCCTCATG 
GAGTGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG CTGAGAGGAA ATTTGATGAG 
GCCATGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTATACAA GCTTCTGGCT 
CACATCAGAC CCCCAGTTTC TAAGAGTGGC TCACACACAG TGGCCGTGAT GAATGTGGGG 
GCCCCGGCTG CAGGCATGAA TGCTGCCGTC CGCTCCACTG TGAGAATTGG CCTCATCCAG 
GGCAACCGAG TGCTGGTTGT GCACGATGGC TTCGAGGGCC TGGCCAAGGG CCAGATTGAG 
GAAGCTGGCT GGAGCTACGT TGGCGGCTGG ACTGGCCAAG GGGGTTCTAA ACTTGGGACT 
AAAAGGACGC TACCCAGGAA GAGCTTCGAG CAGATCAGTG CCAACATCAC TAAGTTCAAC 
ATTCAAGGCC TGATCATCGT TGGGGGCTTC GAGGCTTACA CAGGGGGCCT GGAGCTGATG 
GAGGGCAGGA AGCAGTACGA CGAGCTCTGC ATCCCGTTTG TGGTCATCCC TGCCACGGTC 
TCCAACAACG TCCCTGGCTC AGACTTCAGC GTGGGGGCTG ACACCGCACT CAATACTATC 
TGCATGACCT GTGACCGCAT CAAGCAGTCA GCAGCAGGTA CCAAGCGTCG AGTGTTTATC 
ATTGAAACCA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCAGGACT GGCAGCTGGA 
GCCGATGCGG CCTACATTTT CGAGGAGCCA TTCACCATTC GAGACCTGCA GGTGAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAG 
AAGTGCAATG AGAACTATAG CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGCCACATGC AGCAGGGTGG GAGCCCAACT 
CCATTTGATA GGAATTTTGC CACTAAGATG GGTGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACCCCAGA CTCAGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCCCTGGTC TTTCAACCAG TGACTGAGCT GAAGGAACAG 
ACGGATTTCG AGCACCGCAT CCCCAAGGAA CAGTGGTGGC TGAAGCTGAG GCCCATCCTC 
AAGATCCTAG CCAAGTATGA GATTGACCTG GATACATCAG AGCACGCCCA CCTGGAGCAC 
ATCAGTCGGA AGCGGTCTGG AGAAGCTCCT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_023642167.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023497935.1 Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb66016
Clone ID Related Accession (Same CDS sequence)	XM_023642167.1
Accession Version	XM_023642167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTAAAG AGGAATTACA TCTGAAATTT TTCATGTGTG TGATTCAATC TCGCCAATTA 
GTCAGGACTC CTCAGAGAAC AGAGTGGATC ATGACCCATG AAGAGCACCA TGCAGCCAAA 
ACCCTGGGGA TCGGCAAAGC CATCGCCGTG TTAACCTCTG GTGGAGATGC CCAAGGTATG 
AATGCTGCTG TCAGGGCTGT GGTTCGAGTT GGTATCTTCA CTGGTGCCCG TGTCTTCTTT 
GTCCATGAGG GTTATCAAGG CCTGGTGGAT GGTGGAGACC ACATCAGGGA GGCCACCTGG 
GAGAGCGTTT CGATGATGCT TCAGCTGGGT GGCACAGTGA TTGGAAGTGC CCGGTGCAAG 
GACTTTCGGG AACGAGAGGG ACGACTCCGA GCTGCCCACA ACCTGGTGAA GCTTGGGATT 
ACCAACCTGT GTGTCATTGG GGGCGATGGT AGCCTCACTG GGGCTGACAC CTTCCGTTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAAGCGGGTA AGATCACAGC TGAGGAGGCT 
ACGAAGTCCA ACTACCTGAA CATCGTGGGC CTGGTTGGCT CAATTGACAA TGACTTTTGC 
GGCACTGATA TGACCATTGG CACTGACTCT GCCCTGCACC GGATCATAGA GATTGTAGAC 
GCCATCACTA CTACTGCTCA GAGCCACCAG AGGACGTTCG TGTTAGAGGT GATGGGCCGG 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTCTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAATGTC CACCAGATGA TGACTGGGAG GATCATCTTT GTCGTCGGCT CAGTGAGACA 
AGGACCCGTG GTTCTCGTCT CAACATCATC ATTGTGGCTG AGGGTGCGAT TGACAGGAAT 
GGGAAACCAA TCACCTCAGA ATCCATCAAG GATCTGGTGG TAAAGCGTCT GGGATATGAC 
ACCCGGGTCA CCGTCTTGGG GCATGTGCAG CGAGGTGGGA CCCCGTCAGC CTTTGACCGG 
ATCCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGC GACCCCAGAC 
ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT AATCAGGCTG TGCGGCTGCC CCTCATGGAG 
TGTGTCCAGG TGACCAAAGA TGTGACCAAG GCCATGGCTG AGAGGAAATT TGATGAGGCC 
ATGAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TATACAAGCT TCTGGCTCAC 
ATCAGACCCC CAGTTTCTAA GAGTGGCTCA CACACAGTGG CCGTGATGAA TGTGGGGGCC 
CCGGCTGCAG GCATGAATGC TGCCGTCCGC TCCACTGTGA GAATTGGCCT CATCCAGGGC 
AACCGAGTGC TGGTTGTGCA CGATGGCTTC GAGGGCCTGG CCAAGGGCCA GATTGAGGAA 
GCTGGCTGGA GCTACGTTGG CGGCTGGACT GGCCAAGGGG GTTCTAAACT TGGGACTAAA 
AGGACGCTAC CCAGGAAGAG CTTCGAGCAG ATCAGTGCCA ACATCACTAA GTTCAACATT 
CAAGGCCTGA TCATCGTTGG GGGCTTCGAG GCTTACACAG GGGGCCTGGA GCTGATGGAG 
GGCAGGAAGC AGTACGACGA GCTCTGCATC CCGTTTGTGG TCATCCCTGC CACGGTCTCC 
AACAACGTCC CTGGCTCAGA CTTCAGCGTG GGGGCTGACA CCGCACTCAA TACTATCTGC 
ATGACCTGTG ACCGCATCAA GCAGTCAGCA GCAGGTACCA AGCGTCGAGT GTTTATCATT 
GAAACCATGG GTGGCTACTG TGGCTACCTG GCTACCATGG CAGGACTGGC AGCTGGAGCC 
GATGCGGCCT ACATTTTCGA GGAGCCATTC ACCATTCGAG ACCTGCAGGT GAATGTTGAA 
CATCTGGTGC AAAAGATGAA AACAACTGTG AAAAGGGGCT TGGTGTTAAG GAATGAGAAG 
TGCAATGAGA ACTATAGCAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA TGTGCTTGGC CACATGCAGC AGGGTGGGAG CCCAACTCCA 
TTTGATAGGA ATTTTGCCAC TAAGATGGGT GCCAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATA CCCCAGACTC AGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC CCTGGTCTTT CAACCAGTGA CTGAGCTGAA GGAACAGACG 
GATTTCGAGC ACCGCATCCC CAAGGAACAG TGGTGGCTGA AGCTGAGGCC CATCCTCAAG 
ATCCTAGCCA AGTATGAGAT TGACCTGGAT ACATCAGAGC ACGCCCACCT GGAGCACATC 
AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023497935.1
CDS	10..2442
Translation	MRKEELHLKFFMCVIQSRQLVRTPQRTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKLGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATKSNYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIIEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEDHLCRRLSETRTRGSRLNIIIVAEGAIDRNGKPITSESIKDLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMAERKFDEAMKLRGRSFMNNWEVYKLLAHIRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPRKSFEQISANITKFNIQGLIIVGGFEAYTGGLELMEGRKQYDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICMTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEKCNENYSTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKEQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSEHAHLEHISRKRSGEAPA

Target ORF information:

RefSeq Version	XM_023642167.1
Organism	Equus caballus(horse)
Definition	Equus caballus phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023642167.1

ORF Insert Sequence:

ATGCGTAAAG AGGAATTACA TCTGAAATTT TTCATGTGTG TGATTCAATC TCGCCAATTA 
GTCAGGACTC CTCAGAGAAC AGAGTGGATC ATGACCCATG AAGAGCACCA TGCAGCCAAA 
ACCCTGGGGA TCGGCAAAGC CATCGCCGTG TTAACCTCTG GTGGAGATGC CCAAGGTATG 
AATGCTGCTG TCAGGGCTGT GGTTCGAGTT GGTATCTTCA CTGGTGCCCG TGTCTTCTTT 
GTCCATGAGG GTTATCAAGG CCTGGTGGAT GGTGGAGACC ACATCAGGGA GGCCACCTGG 
GAGAGCGTTT CGATGATGCT TCAGCTGGGT GGCACAGTGA TTGGAAGTGC CCGGTGCAAG 
GACTTTCGGG AACGAGAGGG ACGACTCCGA GCTGCCCACA ACCTGGTGAA GCTTGGGATT 
ACCAACCTGT GTGTCATTGG GGGCGATGGT AGCCTCACTG GGGCTGACAC CTTCCGTTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAAGCGGGTA AGATCACAGC TGAGGAGGCT 
ACGAAGTCCA ACTACCTGAA CATCGTGGGC CTGGTTGGCT CAATTGACAA TGACTTTTGC 
GGCACTGATA TGACCATTGG CACTGACTCT GCCCTGCACC GGATCATAGA GATTGTAGAC 
GCCATCACTA CTACTGCTCA GAGCCACCAG AGGACGTTCG TGTTAGAGGT GATGGGCCGG 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTCTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAATGTC CACCAGATGA TGACTGGGAG GATCATCTTT GTCGTCGGCT CAGTGAGACA 
AGGACCCGTG GTTCTCGTCT CAACATCATC ATTGTGGCTG AGGGTGCGAT TGACAGGAAT 
GGGAAACCAA TCACCTCAGA ATCCATCAAG GATCTGGTGG TAAAGCGTCT GGGATATGAC 
ACCCGGGTCA CCGTCTTGGG GCATGTGCAG CGAGGTGGGA CCCCGTCAGC CTTTGACCGG 
ATCCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGC GACCCCAGAC 
ACCCCAGCCT GCGTGGTGAG CCTCTCTGGT AATCAGGCTG TGCGGCTGCC CCTCATGGAG 
TGTGTCCAGG TGACCAAAGA TGTGACCAAG GCCATGGCTG AGAGGAAATT TGATGAGGCC 
ATGAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TATACAAGCT TCTGGCTCAC 
ATCAGACCCC CAGTTTCTAA GAGTGGCTCA CACACAGTGG CCGTGATGAA TGTGGGGGCC 
CCGGCTGCAG GCATGAATGC TGCCGTCCGC TCCACTGTGA GAATTGGCCT CATCCAGGGC 
AACCGAGTGC TGGTTGTGCA CGATGGCTTC GAGGGCCTGG CCAAGGGCCA GATTGAGGAA 
GCTGGCTGGA GCTACGTTGG CGGCTGGACT GGCCAAGGGG GTTCTAAACT TGGGACTAAA 
AGGACGCTAC CCAGGAAGAG CTTCGAGCAG ATCAGTGCCA ACATCACTAA GTTCAACATT 
CAAGGCCTGA TCATCGTTGG GGGCTTCGAG GCTTACACAG GGGGCCTGGA GCTGATGGAG 
GGCAGGAAGC AGTACGACGA GCTCTGCATC CCGTTTGTGG TCATCCCTGC CACGGTCTCC 
AACAACGTCC CTGGCTCAGA CTTCAGCGTG GGGGCTGACA CCGCACTCAA TACTATCTGC 
ATGACCTGTG ACCGCATCAA GCAGTCAGCA GCAGGTACCA AGCGTCGAGT GTTTATCATT 
GAAACCATGG GTGGCTACTG TGGCTACCTG GCTACCATGG CAGGACTGGC AGCTGGAGCC 
GATGCGGCCT ACATTTTCGA GGAGCCATTC ACCATTCGAG ACCTGCAGGT GAATGTTGAA 
CATCTGGTGC AAAAGATGAA AACAACTGTG AAAAGGGGCT TGGTGTTAAG GAATGAGAAG 
TGCAATGAGA ACTATAGCAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA TGTGCTTGGC CACATGCAGC AGGGTGGGAG CCCAACTCCA 
TTTGATAGGA ATTTTGCCAC TAAGATGGGT GCCAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATA CCCCAGACTC AGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC CCTGGTCTTT CAACCAGTGA CTGAGCTGAA GGAACAGACG 
GATTTCGAGC ACCGCATCCC CAAGGAACAG TGGTGGCTGA AGCTGAGGCC CATCCTCAAG 
ATCCTAGCCA AGTATGAGAT TGACCTGGAT ACATCAGAGC ACGCCCACCT GGAGCACATC 
AGTCGGAAGC GGTCTGGAGA AGCTCCTGCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Annotation of the Protein Coding Regions of the Equine Genome.
PloS one10(6)e0124375(2015)
Hestand MS,Kalbfleisch TS,Coleman SJ,Zeng Z,Liu J,Orlando L,MacLeod JN

Molecular characterization of glycogen synthase 1 and its tissue expression profile with type II hexokinase and muscle-type phosphofructokinase in horses.
Molecular biology reports38(1)461-9(2011 Jan)
Echigoya Y,Okabe H,Itou T,Endo H,Sakai T

Molecular cloning of equine muscle-type phosphofructokinase cDNA.
The Journal of veterinary medical science65(5)645-8(2003 May)
Sato T,Itou T,Sakai T

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PFKM cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム