TNF cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	100033834
Full Name	tumor necrosis factor
Synonyms	TNF-a,TNF-alpha,TNFA,TNFSF2
General protein information	Preferred Names tumor necrosis factor Names tumor necrosis factor cachectin tumor necrosis factor (TNF superfamily, member 2) tumor necrosis factor ligand superfamily member 2 tumor necrosis factor-alpha
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081819.2	NP_001075288.2	tumor necrosis factor
XM_005603490.1	XP_005603547.1	tumor necrosis factor isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative genomic analysis of eutherian tumor necrosis factor ligand genes.
Premzl M
Immunogenetics68(2)125-32(2016 Feb)

The production and distribution of IL-6 and TNF-a in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome.
Basinska K, Marycz K, ?ieszek A, Nicpo? J
Journal of veterinary science16(1)113-20(2015)

Ovarian steroid-dependent tumor necrosis factor-? production and its action on the equine endometrium in vitro.
Sz?stek AZ, Adamowski M, Galv?o AM, Ferreira-Dias GM, Skarzynski DJ
Cytokine67(2)85-91(2014 Jun)

Role of tumor necrosis factor-?, interferon-? and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.
Galv?o AM, Sz?stek AZ, Skarzynski DJ, Ferreira-Dias GM
Cytokine64(1)18-21(2013 Oct)

Cytokines tumor necrosis factor-? and interferon-? participate in modulation of the equine corpus luteum as autocrine and paracrine factors.
Galv?o A, Skarzynski DJ, Sz?stek A, Silva E, Tramontano A, Mollo A, Mateus L, Ferreira-Dias G
Journal of reproductive immunology93(1)28-37(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with equine metabolic syndrome was enhanced while TNF-alpha levels of both groups were comparable.
Title: The production and distribution of IL-6 and TNF-a in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome.

Data indicate that tumor necrosis factor-alpha production is closely related to ovarian steroid actions.
Title: Ovarian steroid-dependent tumor necrosis factor-? production and its action on the equine endometrium in vitro.

These results indicate that in equine corpus luteum, cytokines TNF, IFNG and FASL regulate nitric oxide activity, via eNOS expression modulation.
Title: Role of tumor necrosis factor-?, interferon-? and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.

These data show the presence of cytokines TNF and IFNG, and their receptors, in the equine corpus luteum and indicate their potential involvement in regulation of luteal function.
Title: Cytokines tumor necrosis factor-? and interferon-? participate in modulation of the equine corpus luteum as autocrine and paracrine factors.

These results suggest that basal oxidative stress markers, circulating cytokines and anti-inflammatory neuroendocrine hormones appear to correlate with endurance performance in horses.
Title: Neuroendocrine and non-neuroendocrine markers of inflammation associated with performance in endurance horses.

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb28101	NM_001081819.2 Latest version!	Equus caballus tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OHb11275	XM_005603490.1 Latest version!	Equus caballus tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_001081819.2 ) cDNA ORF clone, Equus caballus(horse) -> NP_001075288.2 Equus caballus tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OHb28101
Clone ID Related Accession (Same CDS sequence)	NM_001081819.2
Accession Version	NM_001081819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Equus caballus(horse)
Product	tumor necrosis factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB035735.1 and EU438779.1. On May 29, 2015 this sequence version replaced NM_001081819.1. ##Evidence-Data-START## Transcript exon combination :: AB035735.1, EU438779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2452530, SAMEA2743238 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACTG AAAGCATGAT CCGAGATGTG GAGCTGGCAG AGGAGGAGCT CGCCAAGAAG 
GCAGGGGGCC CCCAGGGCTC CAGACGGTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTT 
GTCGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTTG GGGTGATCGG CCCCCAGAGG 
GAAGAGCAGT TACCGAATGC CTTCCAGTCA ATCAACCCTC TGGCCCAGAC ACTCAGATCA 
TCTTCTCGAA CCCCAAGTGA CAAGCCTGTA GCCCATGTTG TAGCAAACCC CCAAGCCGAG 
GGGCAGCTCC AGTGGCTGAG TGGGCGTGCA AATGCCCTCC TGGCCAATGG CGTGAAGCTG 
ACAGACAACC AGCTGGTGGT ACCATTGGAT GGGCTGTACC TCATCTACTC CCAGGTCCTC 
TTCAAAGGCC AAGGCTGCCC TTCCACCCAT GTGCTCCTCA CCCACACCAT CAGCCGCTTA 
GCTGTCTCCT ACCCGTCCAA GGTCAACCTC CTCTCTGCCA TCAAGAGCCC TTGCCACACG 
GAGTCCCCAG AGCAGGCTGA AGCCAAGCCC TGGTATGAGC CCATCTACCT GGGAGGAGTC 
TTCCAGCTGG AGAAGGGTGA TCAACTCAGC GCTGAGATCA ATCAGCCCAA CTATCTCGAC 
TTTGCGGAGT CCGGGCAGGT CTACTTTGGG ATCATTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075288.2
CDS	36..740
Translation	MSTESMIRDVELAEEELAKKAGGPQGSRRCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREEQLPNAFQSINPLAQTLRSSSRTPSDKPVAHVVANPQAEGQLQWLSGRANALLANGVKLTDNQLVVPLDGLYLIYSQVLFKGQGCPSTHVLLTHTISRLAVSYPSKVNLLSAIKSPCHTESPEQAEAKPWYEPIYLGGVFQLEKGDQLSAEINQPNYLDFAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_001081819.2
Organism	Equus caballus(horse)
Definition	Equus caballus tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081819.2

ORF Insert Sequence:

ATGAGCACTG AAAGCATGAT CCGAGATGTG GAGCTGGCAG AGGAGGAGCT CGCCAAGAAG 
GCAGGGGGCC CCCAGGGCTC CAGACGGTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTT 
GTCGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTTG GGGTGATCGG CCCCCAGAGG 
GAAGAGCAGT TACCGAATGC CTTCCAGTCA ATCAACCCTC TGGCCCAGAC ACTCAGATCA 
TCTTCTCGAA CCCCAAGTGA CAAGCCTGTA GCCCATGTTG TAGCAAACCC CCAAGCCGAG 
GGGCAGCTCC AGTGGCTGAG TGGGCGTGCA AATGCCCTCC TGGCCAATGG CGTGAAGCTG 
ACAGACAACC AGCTGGTGGT ACCATTGGAT GGGCTGTACC TCATCTACTC CCAGGTCCTC 
TTCAAAGGCC AAGGCTGCCC TTCCACCCAT GTGCTCCTCA CCCACACCAT CAGCCGCTTA 
GCTGTCTCCT ACCCGTCCAA GGTCAACCTC CTCTCTGCCA TCAAGAGCCC TTGCCACACG 
GAGTCCCCAG AGCAGGCTGA AGCCAAGCCC TGGTATGAGC CCATCTACCT GGGAGGAGTC 
TTCCAGCTGG AGAAGGGTGA TCAACTCAGC GCTGAGATCA ATCAGCCCAA CTATCTCGAC 
TTTGCGGAGT CCGGGCAGGT CTACTTTGGG ATCATTGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_005603490.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_005603547.1 Equus caballus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb11275
Clone ID Related Accession (Same CDS sequence)	XM_005603490.1
Accession Version	XM_005603490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009163.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGG GCTCGACCAT CTCCCAGCTG GACTTGAGCC CCTCTGGAAA GGACATCATG 
AGCACTGAAA GCATGATCCG AGATGTGGAG CTGGCAGAGG AGGAGCTCGC CAAGAAGGCA 
GGGGGCCCCC AGGGCTCCAG ACGGTGCTTG TGCCTCAGCC TCTTCTCCTT CCTCCTTGTC 
GCAGGAGCCA CCACGCTCTT CTGCCTGCTG CACTTTGGGG TGATCGGCCC CCAGAGGGAA 
GAGTTACCGA ATGCCTTCCA GTCAATCAAC CCTCTGGCCC AGACACTCAG ATCATCTTCT 
CGAACCCCAA GTGACAAGCC TGTAGCCCAT GTTGTAGCAA ACCCCCAAGC CGAGGGGCAG 
CTCCAGTGGC TGAGTGGGCG TGCAAATGCC CTCCTGGCCA ATGGCGTGAA GCTGACAGAC 
AACCAGCTGG TGGTACCATT GGATGGGCTG TACCTCATCT ACTCCCAGGT CCTCTTCAAA 
GGCCAAGGCT GCCCTTCCAC CCATGTGCTC CTCACCCACA CCATCAGCCG CTTAGCTGTC 
TCCTACCCGT CCAAGGTCAA CCTCCTCTCT GCCATCAAGA GCCCTTGCCA CACGGAGTCC 
CCAGAGCAGG CTGAAGCCAA GCCCTGGTAT GAGCCCATCT ACCTGGGAGG AGTCTTCCAG 
CTGGAGAAGG GTGATCAACT CAGCGCTGAG ATCAATCAGC CCAACTATCT CGACTTTGCG 
GAGTCCGGGC AGGTCTACTT TGGGATCATT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005603547.1
CDS	115..873
Translation	MPQGSTISQLDLSPSGKDIMSTESMIRDVELAEEELAKKAGGPQGSRRCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREELPNAFQSINPLAQTLRSSSRTPSDKPVAHVVANPQAEGQLQWLSGRANALLANGVKLTDNQLVVPLDGLYLIYSQVLFKGQGCPSTHVLLTHTISRLAVSYPSKVNLLSAIKSPCHTESPEQAEAKPWYEPIYLGGVFQLEKGDQLSAEINQPNYLDFAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_005603490.1
Organism	Equus caballus(horse)
Definition	Equus caballus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005603490.1

ORF Insert Sequence:

ATGCCCCAGG GCTCGACCAT CTCCCAGCTG GACTTGAGCC CCTCTGGAAA GGACATCATG 
AGCACTGAAA GCATGATCCG AGATGTGGAG CTGGCAGAGG AGGAGCTCGC CAAGAAGGCA 
GGGGGCCCCC AGGGCTCCAG ACGGTGCTTG TGCCTCAGCC TCTTCTCCTT CCTCCTTGTC 
GCAGGAGCCA CCACGCTCTT CTGCCTGCTG CACTTTGGGG TGATCGGCCC CCAGAGGGAA 
GAGTTACCGA ATGCCTTCCA GTCAATCAAC CCTCTGGCCC AGACACTCAG ATCATCTTCT 
CGAACCCCAA GTGACAAGCC TGTAGCCCAT GTTGTAGCAA ACCCCCAAGC CGAGGGGCAG 
CTCCAGTGGC TGAGTGGGCG TGCAAATGCC CTCCTGGCCA ATGGCGTGAA GCTGACAGAC 
AACCAGCTGG TGGTACCATT GGATGGGCTG TACCTCATCT ACTCCCAGGT CCTCTTCAAA 
GGCCAAGGCT GCCCTTCCAC CCATGTGCTC CTCACCCACA CCATCAGCCG CTTAGCTGTC 
TCCTACCCGT CCAAGGTCAA CCTCCTCTCT GCCATCAAGA GCCCTTGCCA CACGGAGTCC 
CCAGAGCAGG CTGAAGCCAA GCCCTGGTAT GAGCCCATCT ACCTGGGAGG AGTCTTCCAG 
CTGGAGAAGG GTGATCAACT CAGCGCTGAG ATCAATCAGC CCAACTATCT CGACTTTGCG 
GAGTCCGGGC AGGTCTACTT TGGGATCATT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative genomic analysis of eutherian tumor necrosis factor ligand genes.
Immunogenetics68(2)125-32(2016 Feb)
Premzl M

The production and distribution of IL-6 and TNF-a in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome.
Journal of veterinary science16(1)113-20(2015)
Basinska K,Marycz K,?ieszek A,Nicpo? J

Ovarian steroid-dependent tumor necrosis factor-? production and its action on the equine endometrium in vitro.
Cytokine67(2)85-91(2014 Jun)
Sz?stek AZ,Adamowski M,Galv?o AM,Ferreira-Dias GM,Skarzynski DJ

Role of tumor necrosis factor-?, interferon-? and Fas-ligand on in vitro nitric oxide activity in the corpus luteum.
Cytokine64(1)18-21(2013 Oct)
Galv?o AM,Sz?stek AZ,Skarzynski DJ,Ferreira-Dias GM

Cytokines tumor necrosis factor-? and interferon-? participate in modulation of the equine corpus luteum as autocrine and paracrine factors.
Journal of reproductive immunology93(1)28-37(2012 Jan)
Galv?o A,Skarzynski DJ,Sz?stek A,Silva E,Tramontano A,Mollo A,Mateus L,Ferreira-Dias G