crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLi136770	XM_029493567.1 Latest version!	Echeneis naucrates cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_029493567.1 ) cDNA ORF clone, -> XP_029349427.1 Echeneis naucrates cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OLi136770
Clone ID Related Accession (Same CDS sequence)	XM_029493567.1
Accession Version	XM_029493567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-15
Organism	Echeneis naucrates(live sharksucker)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042532.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Echeneis naucrates Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCA AGTGCTCCGC CGTGATGTTG AACCTCCTCT TCCTGGCCCG AGCGCTCCTG 
GCTCAGGAGT GCACGCCGTG TAACCTCCGG ACGTGTCCGA TCCAGAGCCC GCACGGCTGC 
GGGGCCGGCC GGACCTTGGC CCGGGATCCG TGCGGATGCT GCGACCAGTG CAGCCGCCTG 
GAGCTGGAGC CCTGCGGCGG GGCGGGCTGG ACTCACGGCT ACTGCGCCCT GGGGCTGACC 
TGCGCCTCGC TAAACCAAAC CGGACCTGCC ACCATCCCTG AAATTGGAGT ATGTAAAGTT 
CTCCCTGAGT TTCCAGAGGC AGGTCTGGAG GACGAGCGCT GTCCCCTGAT AACTGGCTGC 
GACAGAGCTG GAGGACAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG TTCTTTCTCC 
TATCCAGACC AAGAAACCTG CATGAAGGCT AGTAAGTCAG AGGGTCGGAA GCACGAACAC 
CGGGAAAGAC ACAGAGAGAA GTATGTGGGA CCATCCAACC CGGCCTGCAT GTTCTCTGGT 
TGTAACCTGA CTGCTGAGGG CTGTGTGTGT GAATCTCAGA GCTGCCACCA TCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCT GCAGAGGAGC TGAGGTGTGC AGGGGTGACG 
TGCCCTGCCC TGCAGGTGCC CTCCTGCCCC CACGGTTCAG TCCTGACCAA GAGCTACACA 
CCCCCTGCTG GCTGCTGCCC ATCCATACCT CCCCAGTGCA CTTGCGACCT CCGTGCCTGC 
CAAAAGCCCC AGTGCCCGAG CGGACAGCGA GCTGTGCTGG TCAGCCAGGC CAGCGGTCAG 
CCAGGAGACT GCTGTGATGT CTTTGAGTGC CAGAAAGTCT CTCCAAAGTG CGTCCACAAT 
GGGAAGGAGT TCACCGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT TTGTCAATGC 
AGAGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGCGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCCG AGGGCGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTGCT GTCGATGGAC 
AGCACGAAGG CCAGCTGCTG GGTGAACAAT AAGCTCAGAG CTCATGAGGA ACAGTGGAAA 
GAGGACGACT GCACTTTTTG CCAGTGTGTG GATGGAGAAC CTCACTGCAC AGCCATGGCC 
TGCAAGCAGA GCTGCCAAAA CCCTGTCAAG ATACCTGGGG AGTGCTGCCC TTTCTGCGAA 
GAACCTTCTT ATGAGACAGT CAGCCCCATT TTATGTCCTC CCCTGGAAAA CTGCTCGCTG 
TCAGGGAATG AATGTCCCTA CGGCTTCCAG CAAGACAAAA ATGGATGTCT GCTCTGCCAG 
TGTCTCAACA ATGACTCCTG CCCTGCTCTG GGAAAATACT GTTCCCTGCA ATGCCCGATG 
GGGTATGAGA GGGACGACTT TGGCTGTGAG GTGTGTGAGT GCAGCATCCC AGTACCCAAG 
TGCCGACCTC TGACCTGCAC TAAGATCTGC CCCTATGGAT ATGTACGGAA CAAGCATGGC 
TGTGAGATGT GCCGTTGCGT CAAGTGTCCC CCCTTCACCT GCGACAAGCA CTGCAGCGAT 
GGCTACCGAC AGAACCGGAG GGGCTGCTCG ATCTGCATGT GTAAAGAAAG TGGTCACGTC 
CCAAGCACCC CTGCCCCAAC CCCAAAAACC AGCTACTGCC TCACCTCCAA TGGGCATCGG 
TACGAGGAAG GTGACGGCTG GCATGATGGC TGCCGCGACT GCTACTGCCA CTCTGGCCGG 
GAGATGTGCG TGCTCATTTC ATGTCCTGTA CCCAGCTGTT CAAATCCAGT GGTCAGGGCT 
GACCAGTGTT GCCCCACATG TGAGGATGAG TCAGGCAGTG GTCAACCAGA AGGAATGGAC 
ATGGTGGTGT GCAGAGCACC TGGGGGTGAA TTTTACGTGG AGGGGGAGAC TTGGAACCTG 
GACGAGTGCA CACGATGCAC CTGCAGGAAG GGACGTGTCC TGTGCGACAC AGAAGTGTGT 
CCCCCAGCTC TCTGCCAGAC CCCAGTCAGG AACAAGGACA CCTGTTGCCA CGTATGCCCA 
GAGGAGACAC TGAGCCCCCT GCTGCCAGTG AACAACAGCC AGCAGGTGTA CTGCATAACC 
GGCGACGGCG ACGTGCTGCT CGCTGGAGAC TCCTGGAAGG CGAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCCGGCTGC CAACTGCAGG 
GTGCCCGTCT TACGCAAGGG CCAGTGCTGC CCACACTGTC TCGAAATTAC AACGTCGGTG 
CCAGTGATGG TGTCTACCAA CGCCCCCAAG ACGACGGCTA AAGCTACAAC AGAGAGTGCG 
ACGTGGATCA CCACAGTCTC TGTACCATCC ATCACCGCTG ATACAGATGA ATCAGCTCTG 
GGTGAGAATT ACCCCAGCCA AACAGAGATG GCTCTCATCT ACCAATCAGC TGCCTGGATC 
CTGGCAGGTC TCCTCCTGGC CATCATCATC TTCCTCATTG TAGCGGTGCT CATCAATAAG 
AAGAAGAAGT GGGTGCAGAT GTCCTGCTAC AGTGCACCAA AGAAGACGGT GATCCTGAAG 
AAGCATGTAA ACAAGAACTC AGTTGTCTAC ATGGAGCCTT CCAAGGAGAA CAAGTTTCAG 
AACGTAAAGA ACGACTGCGG TATCATCCAC TTCTCTCCTG AAATGAGTTC CCCCTGCACA 
GACAAGATGA CCATCCCCAG GGCCAAGCTG GGCAACGATT GGACGTCACG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029349427.1
CDS	235..3048
Translation	MLGKCSAVMLNLLFLARALLAQECTPCNLRTCPIQSPHGCGAGRTLARDPCGCCDQCSRLELEPCGGAGWTHGYCALGLTCASLNQTGPATIPEIGVCKVLPEFPEAGLEDERCPLITGCDRAGGQCVCDARHSCLGSFSYPDQETCMKASKSEGRKHEHRERHREKYVGPSNPACMFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPALQVPSCPHGSVLTKSYTPPAGCCPSIPPQCTCDLRACQKPQCPSGQRAVLVSQASGQPGDCCDVFECQKVSPKCVHNGKEFTEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPILCPPLENCSLSGNECPYGFQQDKNGCLLCQCLNNDSCPALGKYCSLQCPMGYERDDFGCEVCECSIPVPKCRPLTCTKICPYGYVRNKHGCEMCRCVKCPPFTCDKHCSDGYRQNRRGCSICMCKESGHVPSTPAPTPKTSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSNPVVRADQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPVRNKDTCCHVCPEETLSPLLPVNNSQQVYCITGDGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEITTSVPVMVSTNAPKTTAKATTESATWITTVSVPSITADTDESALGENYPSQTEMALIYQSAAWILAGLLLAIIIFLIVAVLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKNDCGIIHFSPEMSSPCTDKMTIPRAKLGNDWTSR

Target ORF information:

RefSeq Version	XM_029493567.1
Organism	Echeneis naucrates(live sharksucker)
Definition	Echeneis naucrates cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029493567.1

ORF Insert Sequence:

ATGTTGGGCA AGTGCTCCGC CGTGATGTTG AACCTCCTCT TCCTGGCCCG AGCGCTCCTG 
GCTCAGGAGT GCACGCCGTG TAACCTCCGG ACGTGTCCGA TCCAGAGCCC GCACGGCTGC 
GGGGCCGGCC GGACCTTGGC CCGGGATCCG TGCGGATGCT GCGACCAGTG CAGCCGCCTG 
GAGCTGGAGC CCTGCGGCGG GGCGGGCTGG ACTCACGGCT ACTGCGCCCT GGGGCTGACC 
TGCGCCTCGC TAAACCAAAC CGGACCTGCC ACCATCCCTG AAATTGGAGT ATGTAAAGTT 
CTCCCTGAGT TTCCAGAGGC AGGTCTGGAG GACGAGCGCT GTCCCCTGAT AACTGGCTGC 
GACAGAGCTG GAGGACAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG TTCTTTCTCC 
TATCCAGACC AAGAAACCTG CATGAAGGCT AGTAAGTCAG AGGGTCGGAA GCACGAACAC 
CGGGAAAGAC ACAGAGAGAA GTATGTGGGA CCATCCAACC CGGCCTGCAT GTTCTCTGGT 
TGTAACCTGA CTGCTGAGGG CTGTGTGTGT GAATCTCAGA GCTGCCACCA TCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCT GCAGAGGAGC TGAGGTGTGC AGGGGTGACG 
TGCCCTGCCC TGCAGGTGCC CTCCTGCCCC CACGGTTCAG TCCTGACCAA GAGCTACACA 
CCCCCTGCTG GCTGCTGCCC ATCCATACCT CCCCAGTGCA CTTGCGACCT CCGTGCCTGC 
CAAAAGCCCC AGTGCCCGAG CGGACAGCGA GCTGTGCTGG TCAGCCAGGC CAGCGGTCAG 
CCAGGAGACT GCTGTGATGT CTTTGAGTGC CAGAAAGTCT CTCCAAAGTG CGTCCACAAT 
GGGAAGGAGT TCACCGAGGG AGAGGTGTAC CGCATGGACC CCTGCTGGCT TTGTCAATGC 
AGAGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGCGCTG AGCTGGACTG TGAAAACTTC 
TACATTCCCG AGGGCGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTGCT GTCGATGGAC 
AGCACGAAGG CCAGCTGCTG GGTGAACAAT AAGCTCAGAG CTCATGAGGA ACAGTGGAAA 
GAGGACGACT GCACTTTTTG CCAGTGTGTG GATGGAGAAC CTCACTGCAC AGCCATGGCC 
TGCAAGCAGA GCTGCCAAAA CCCTGTCAAG ATACCTGGGG AGTGCTGCCC TTTCTGCGAA 
GAACCTTCTT ATGAGACAGT CAGCCCCATT TTATGTCCTC CCCTGGAAAA CTGCTCGCTG 
TCAGGGAATG AATGTCCCTA CGGCTTCCAG CAAGACAAAA ATGGATGTCT GCTCTGCCAG 
TGTCTCAACA ATGACTCCTG CCCTGCTCTG GGAAAATACT GTTCCCTGCA ATGCCCGATG 
GGGTATGAGA GGGACGACTT TGGCTGTGAG GTGTGTGAGT GCAGCATCCC AGTACCCAAG 
TGCCGACCTC TGACCTGCAC TAAGATCTGC CCCTATGGAT ATGTACGGAA CAAGCATGGC 
TGTGAGATGT GCCGTTGCGT CAAGTGTCCC CCCTTCACCT GCGACAAGCA CTGCAGCGAT 
GGCTACCGAC AGAACCGGAG GGGCTGCTCG ATCTGCATGT GTAAAGAAAG TGGTCACGTC 
CCAAGCACCC CTGCCCCAAC CCCAAAAACC AGCTACTGCC TCACCTCCAA TGGGCATCGG 
TACGAGGAAG GTGACGGCTG GCATGATGGC TGCCGCGACT GCTACTGCCA CTCTGGCCGG 
GAGATGTGCG TGCTCATTTC ATGTCCTGTA CCCAGCTGTT CAAATCCAGT GGTCAGGGCT 
GACCAGTGTT GCCCCACATG TGAGGATGAG TCAGGCAGTG GTCAACCAGA AGGAATGGAC 
ATGGTGGTGT GCAGAGCACC TGGGGGTGAA TTTTACGTGG AGGGGGAGAC TTGGAACCTG 
GACGAGTGCA CACGATGCAC CTGCAGGAAG GGACGTGTCC TGTGCGACAC AGAAGTGTGT 
CCCCCAGCTC TCTGCCAGAC CCCAGTCAGG AACAAGGACA CCTGTTGCCA CGTATGCCCA 
GAGGAGACAC TGAGCCCCCT GCTGCCAGTG AACAACAGCC AGCAGGTGTA CTGCATAACC 
GGCGACGGCG ACGTGCTGCT CGCTGGAGAC TCCTGGAAGG CGAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCCGGCTGC CAACTGCAGG 
GTGCCCGTCT TACGCAAGGG CCAGTGCTGC CCACACTGTC TCGAAATTAC AACGTCGGTG 
CCAGTGATGG TGTCTACCAA CGCCCCCAAG ACGACGGCTA AAGCTACAAC AGAGAGTGCG 
ACGTGGATCA CCACAGTCTC TGTACCATCC ATCACCGCTG ATACAGATGA ATCAGCTCTG 
GGTGAGAATT ACCCCAGCCA AACAGAGATG GCTCTCATCT ACCAATCAGC TGCCTGGATC 
CTGGCAGGTC TCCTCCTGGC CATCATCATC TTCCTCATTG TAGCGGTGCT CATCAATAAG 
AAGAAGAAGT GGGTGCAGAT GTCCTGCTAC AGTGCACCAA AGAAGACGGT GATCCTGAAG 
AAGCATGTAA ACAAGAACTC AGTTGTCTAC ATGGAGCCTT CCAAGGAGAA CAAGTTTCAG 
AACGTAAAGA ACGACTGCGG TATCATCCAC TTCTCTCCTG AAATGAGTTC CCCCTGCACA 
GACAAGATGA CCATCCCCAG GGCCAAGCTG GGCAACGATT GGACGTCACG CTAG