fray cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fray
Entrez Gene ID	44233
Full Name	frayed
Synonyms	4624,CG7693,Dmel\CG7693,Dosr1,Fray,STE20-like/SPAK,dOSR1,dSTRAD,l(3)07551,lag
General protein information	Preferred Names frayed Names CG7693 gene product from transcript CG7693-RG CG7693-PA CG7693-PB CG7693-PC CG7693-PE CG7693-PF CG7693-PG CG7693-PH CG7693-PI Ste20-like Ste20-like protein kinase fray-PA fray-PB fray-PC fray-PE fray-PF fray-PG fray-PH fray-PI laggard oxidative-stress-responsive kinase 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91B4-91B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300466.1	NP_001287395.1	frayed, isoform G
NM_169823.2	NP_732352.1	frayed, isoform B
NM_001144605.2	NP_001138077.1	frayed, isoform C
NM_001144607.2	NP_001138079.1	frayed, isoform F
NM_001316520.1	NP_001303449.1	frayed, isoform H
NM_057960.4	NP_477308.1	frayed, isoform A
NM_001144606.1	NP_001138078.1	frayed, isoform E
NM_001316521.1	NP_001303450.1	frayed, isoform I

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	OXSR1	XP_418527.1
Drosophila melanogaster (fruit fly)	fray	NP_732352.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006715	XP_309028.4
Homo sapiens (human)	OXSR1	NP_005100.1
Canis lupus familiaris (dog)	OXSR1	XP_849715.1
Pan troglodytes (chimpanzee)	OXSR1	XP_526174.2
Bos taurus (cattle)	OXSR1	NP_001069360.2
Caenorhabditis elegans (roundworm)	gck-3	NP_507517.2
	OXSR1	XP_001087567.1
Mus musculus (house mouse)	Oxsr1	NP_598746.2
Rattus norvegicus (Norway rat)	Oxsr1	NP_001101664.1
Xenopus tropicalis (tropical clawed frog)	stk39	NP_001001224.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila WNK-SPAK/OSR1-NKCC cascade is an essential molecular pathway for osmoregulation.
Title: Hypotonicity stimulates potassium flux through the WNK-SPAK/OSR1 kinase cascade and the Ncc69 sodium-potassium-2-chloride cotransporter in the Drosophila renal tubule.

This study proposed that normally functioning nerves generate extracellular solutes that are removed by Ncc69 under the control of Fray.
Title: Drosophila glia use a conserved cotransporter mechanism to regulate extracellular volume.

We suggest from those results that Mo25 and Fray operate together or in the same pathway in Drosophila asymmetric processes, and that their function counterbalances Lkb1.
Title: The GC kinase Fray and Mo25 regulate Drosophila asymmetric divisions.

The following fray gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fray cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06830	NM_001144606.1 Latest version!	Drosophila melanogaster frayed (fray), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_169823.2 Latest version!	Drosophila melanogaster frayed (fray), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_001144607.2 Latest version!	Drosophila melanogaster frayed (fray), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_057960.4 Latest version!	Drosophila melanogaster frayed (fray), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_001316520.1 Latest version!	Drosophila melanogaster frayed (fray), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_001300466.1 Latest version!	Drosophila melanogaster frayed (fray), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_001144605.2 Latest version!	Drosophila melanogaster frayed (fray), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06830	NM_001316521.1 Latest version!	Drosophila melanogaster frayed (fray), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fray ( NM_001300466.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287395.1 Drosophila melanogaster frayed (fray), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_001300466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287395.1
CDS	820..2478
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_001300466.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300466.1

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_169823.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732352.1 Drosophila melanogaster frayed (fray), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_169823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_169823.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732352.1
CDS	546..2204
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_169823.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169823.2

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_001144605.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138077.1 Drosophila melanogaster frayed (fray), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_001144605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_001144605.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138077.1
CDS	867..2525
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_001144605.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144605.2

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_001144607.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138079.1 Drosophila melanogaster frayed (fray), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_001144607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_001144607.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138079.1
CDS	620..2278
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_001144607.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144607.2

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_001316520.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303449.1 Drosophila melanogaster frayed (fray), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_001316520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303449.1
CDS	721..2379
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_001316520.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316520.1

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_057960.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477308.1 Drosophila melanogaster frayed (fray), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_057960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_057960.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477308.1
CDS	885..2543
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_057960.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057960.4

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_001144606.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138078.1 Drosophila melanogaster frayed (fray), transcript variant E, mRNA.

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CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_001144606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138078.1
CDS	527..2185
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_001144606.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144606.1

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fray ( NM_001316521.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303450.1 Drosophila melanogaster frayed (fray), transcript variant I, mRNA.

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CloneID	OFa06830
Clone ID Related Accession (Same CDS sequence)	NM_001300466.1 , NM_169823.2 , NM_001144605.2 , NM_001144607.2 , NM_001316520.1 , NM_057960.4 , NM_001144606.1 , NM_001316521.1
Accession Version	NM_001316521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frayed, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303450.1
CDS	630..2288
Translation	MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDELLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHSNGQIHRDIKAGNILIGDDGTIQIADFGVSAWLATGRDLSRQKVRHTFVGTPCWMAPEVMEQDHGYDFKADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAYGKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWSSEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAGIAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMKTITFQLSSGVQPGEVPDERSLVGYAQISITD

Target ORF information:

RefSeq Version	NM_001316521.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frayed (fray), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316521.1

ORF Insert Sequence:

ATGACCTCCA TACCCGCCAA TCTGTCTAGC AATAATGTTG CTGGTGCAGC AACCTTACCA 
GGAGCTGCGG CACCGCCAGA GAAATATACA TGGCCGAACT CCAAGGACGA TTACGAGCTG 
AGGGACGTCA TTGGTGTGGG TGCCACAGCC GTTGTCCATG GGGCTTACTG TATTCCAAGG 
AACGAGAAGT GCGCCATTAA GCGCATCAAC CTGGAGAAGT GGAACACATC GATGGACGAG 
CTGCTCAAGG AGATTCAGGC CATGTCCTCC TGTTTTCACG AGAACGTGGT GACCTATCAC 
ACCTCATTTG TGGTTAGGGA AGAGCTCTGG TTGGTGCTAA GACTTCTAGA AGGCGGTTCT 
TTGCTGGACA TCATCAAGCA CAAGATGCGA ACGTCCAACT GCAAACAGGG TGTCTTCGAC 
GAAGCCACCA TTGCCACGGT ACTGAAGGAA GTCCTAAAGG GATTGGAATA TTTCCATTCG 
AACGGGCAAA TCCATCGTGA TATCAAGGCC GGCAATATTT TGATTGGTGA CGATGGCACC 
ATTCAAATCG CTGACTTCGG TGTGAGTGCA TGGCTGGCCA CTGGCAGGGA TTTGTCCAGG 
CAGAAAGTGC GCCACACCTT TGTGGGCACA CCGTGTTGGA TGGCTCCCGA GGTGATGGAG 
CAGGATCATG GCTATGACTT TAAGGCGGAC ATTTGGTCCT TTGGAATAAC AGCTATCGAA 
ATGGCCACGG GCACTGCACC ATACCATAAA TACCCGCCCA TGAAGGTGCT AATGCTGACC 
CTGCAAAATG ATCCGCCCAC CTTGGACACG GGTGCGGACG ATAAAGATCA ATATAAGGCG 
TATGGCAAGA CATTCCGCAA GATGATCGTC GAGTGCTTGC AAAAGGAGCC ATCGAAACGT 
CCCACGGCTA GTGAGCTTTT GAAGCACGCT TTCTTCAAGA AGGCCAAGGA TCGCAAATAT 
CTCACCCAGA CGTTGCTGCA ATCTGGGCCC AGTATGGAGA CCAGGGTTCA CAAGGCGGCG 
AAACGCCAAC CAGGAGCCTC GGGCCGTTTG CATCGCACGG TGACAGGCGA ATGGGTCTGG 
TCTAGCGAAG AGGAGGACAA TGGTGGCTCT GCCACCGGTT CCGGAACAGG AGACAGAAAA 
CATCCATCTT CGGACTCGGA TTCCGAGGAT AGACCCATGA ATCGATTGGA AAGAGCCGAC 
TCATCGGACA GCGACCGGGA AGAGCCTTCT CCCGAAATAA CCCACAGTGT TTCCTCAGCC 
ACAGTGACGC CTGGAGCTCC AGCTGCTGCT GGTGCTGGTG CAGCCCAAGA GCTAACAGCT 
GGCATTGCTC AATTACCACT ACCTAGCGAA GCGGCCGGCG AGGCGCCACC AGTCAATCTT 
GTCCTTCGCA TGCGCAACTT GCGACGTGAA CTTCACGACA TTCGCTTCGA GTTCGTTGTG 
GGCAAGGACA CTGCCGAGGG TATCGCTACA GAACTGGTAG ACGCCGGACT CGTTGATGCG 
CTGGATACCC AACCGATGGC AACGCATCTG GATCAACTGA TAGCTGCCAG TGCAACGATG 
AAAACGATTA CTTTCCAATT GAGCTCCGGC GTGCAGCCGG GCGAAGTGCC CGACGAACGA 
TCACTGGTGG GATATGCGCA GATCTCCATC ACGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fray cDNA ORF clone, Drosophila melanogaster(fruit fly)

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