ect cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ect
Entrez Gene ID	44135
Full Name	ectodermal
Synonyms	CG11965,CG6611,DROECT,Difl,Dmel\CG6611,Ifl
General protein information	Preferred Names ectodermal Names CG6611 gene product from transcript CG6611-RA CG6611-PA CG6611-PB CG6611-PD CG6611-PE CG6611-PF Interferon-like protein ect-PA ect-PB ect-PD ect-PE ect-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67D10-67D10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300090.1	NP_001287019.1	ectodermal, isoform F
NM_079967.6	NP_524706.2	ectodermal, isoform A
NM_168400.3	NP_729596.1	ectodermal, isoform B
NM_168401.3	NP_729597.2	ectodermal, isoform D
NM_001274748.1	NP_001261677.1	ectodermal, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ect gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ect cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20466	NM_168401.3 Latest version!	Drosophila melanogaster ectodermal (ect), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa19369	NM_001274748.1 Latest version!	Drosophila melanogaster ectodermal (ect), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa20465	NM_079967.6 Latest version!	Drosophila melanogaster ectodermal (ect), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa20465	NM_168400.3 Latest version!	Drosophila melanogaster ectodermal (ect), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa22208	NM_001300090.1 Latest version!	Drosophila melanogaster ectodermal (ect), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ect ( NM_168401.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729597.2 Drosophila melanogaster ectodermal (ect), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa20466
Clone ID Related Accession (Same CDS sequence)	NM_168401.3
Accession Version	NM_168401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ectodermal, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168401.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTAT CGCCGGTGAC 
GACGACGAGG AGGCCGACGA TGATGATGAC GATGACGACG ATGACATTAT CGGCGATGAC 
ATCATCGAGG CCCGCCGTGA AGCACGTGAG TCCAAATCGA AGTCGGAAAC GGCGACGAAA 
GAAACCAAGG CCAAGAAATC AACAAGTCCC TCAAAGAAGG CCAAGAAAGA GCTGAAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729597.2
CDS	191..1033
Translation	MKFVILLCVLSALLLQIEASPLQRLSRSERAVSRQQTVNNEVAPAVAPASDDDDDDDDEDDDDEPDLGDLIDDDDDDDEEEDDDEDEEDDTPQGQAAPAATASDDDEEEDDDDDDYLDRLFDDILGDDEDEDDDDETPAATAQQSAVAAAPAQTLDEVAPAAASSVSAGISDGVDLPAESGNAAEVLAAGNAADESVSAAVAPAQNAAASSSNNEGIAGDDDEEADDDDDDDDDDIIGDDIIEARREARESKSKSETATKETKAKKSTSPSKKAKKELKH

Target ORF information:

RefSeq Version	NM_168401.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ectodermal (ect), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168401.3

ORF Insert Sequence:

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTAT CGCCGGTGAC 
GACGACGAGG AGGCCGACGA TGATGATGAC GATGACGACG ATGACATTAT CGGCGATGAC 
ATCATCGAGG CCCGCCGTGA AGCACGTGAG TCCAAATCGA AGTCGGAAAC GGCGACGAAA 
GAAACCAAGG CCAAGAAATC AACAAGTCCC TCAAAGAAGG CCAAGAAAGA GCTGAAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect ( NM_001274748.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261677.1 Drosophila melanogaster ectodermal (ect), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa19369
Clone ID Related Accession (Same CDS sequence)	NM_001274748.1
Accession Version	NM_001274748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ectodermal, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTGA CCTGAAGAAC 
AACGTCATCG ATATGTCCAC GGATGCTTTC CAGGCCCGCC ACAACCACTT CATCGATGCC 
ATCTTCTCGA GGATCAATCG CATTGTTTCG GAAAACTATG ATCCCTATGT GGTCAATCTA 
ACGGGTCGCT CCGTTTTGCC AAATGCCGCC AGTGGGTCAA ATGTGAAGGC CGCTAACAAG 
AAGCAATCGA CGAGCAGCAA GGTCGGAGGT CACAAGAAGC TGAAGAACAC GCGCTCGGAG 
CCCCGTTCCG TCAATTCGAA CCAGGAGGCA CGGGAGGCTG CGGTGAAGCT GCAGGAGCAA 
TTGACGCAAT TACAGTCCGA GCTGGCCATC AAAGCCGTGG AGAAGAGGCC CAGCCCCACA 
TCGCCTGCCA CCGAGTCCGA GTCCGATTCC GGATCACCGA AGGCGGAAAC GCGCACCGTT 
TCGGGCTCAA AAACGGGCCA AAAGAAGTCC TCGAACAAGC AGAACACGAA AAAGGCGTCG 
GGCCTGAAAT CAGGAAACTA CAATCAGCCA GCGGGGGCCA GCAAGGCGGG AGCCTCCAGC 
GCTGGTGCAG TGGAAAAGCT GCAAAAGGCC GAGGGCAGGC TCTCCGGACT GGCATCCTTG 
AAGCGCGTGG GAAATGTCAA GGTGATCAGC GATGCCGAGG GACGCAACTC GACGATCAAG 
GCCAAGTTCA CGCTGGGTCC CTTGGTTCTG CGGGTGGAGA AGTCCTTTAA GAAGGGCAGC 
GTGAGGAGCG TGAAGAGTGC GACCGCCAGG ACGAACGAGA TGATGGGTCG CATCAAGTTC 
AGCGTGCTCG ATGATCGGGC CACCCTGATG TCCATCAAGG TGCAGCAGCC CAAGCAGGTG 
GAGGTGGAGA GCAAGGACAA TCACGACCGC ACCCGCGAAT TTGTGTGGCG ACGCACGCCC 
AAAATTGCCA AATTGGTCAA CGAGAAACTG AAATTGGCAG CCGAATCCTT GTTTGCCCCA 
CAGGGCGTCG AGGTTGTCAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261677.1
CDS	134..1780
Translation	MKFVILLCVLSALLLQIEASPLQRLSRSERAVSRQQTVNNEVAPAVAPASDDDDDDDDEDDDDEPDLGDLIDDDDDDDEEEDDDEDEEDDTPQGQAAPAATASDDDEEEDDDDDDYLDRLFDDILGDDEDEDDDDETPAATAQQSAVAAAPAQTLDEVAPAAASSVSAGISDGVDLPAESGNAAEVLAAGNAADESVSAAVAPAQNAAASSSNNEGDLKNNVIDMSTDAFQARHNHFIDAIFSRINRIVSENYDPYVVNLTGRSVLPNAASGSNVKAANKKQSTSSKVGGHKKLKNTRSEPRSVNSNQEAREAAVKLQEQLTQLQSELAIKAVEKRPSPTSPATESESDSGSPKAETRTVSGSKTGQKKSSNKQNTKKASGLKSGNYNQPAGASKAGASSAGAVEKLQKAEGRLSGLASLKRVGNVKVISDAEGRNSTIKAKFTLGPLVLRVEKSFKKGSVRSVKSATARTNEMMGRIKFSVLDDRATLMSIKVQQPKQVEVESKDNHDRTREFVWRRTPKIAKLVNEKLKLAAESLFAPQGVEVVRL

Target ORF information:

RefSeq Version	NM_001274748.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ectodermal (ect), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274748.1

ORF Insert Sequence:

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTGA CCTGAAGAAC 
AACGTCATCG ATATGTCCAC GGATGCTTTC CAGGCCCGCC ACAACCACTT CATCGATGCC 
ATCTTCTCGA GGATCAATCG CATTGTTTCG GAAAACTATG ATCCCTATGT GGTCAATCTA 
ACGGGTCGCT CCGTTTTGCC AAATGCCGCC AGTGGGTCAA ATGTGAAGGC CGCTAACAAG 
AAGCAATCGA CGAGCAGCAA GGTCGGAGGT CACAAGAAGC TGAAGAACAC GCGCTCGGAG 
CCCCGTTCCG TCAATTCGAA CCAGGAGGCA CGGGAGGCTG CGGTGAAGCT GCAGGAGCAA 
TTGACGCAAT TACAGTCCGA GCTGGCCATC AAAGCCGTGG AGAAGAGGCC CAGCCCCACA 
TCGCCTGCCA CCGAGTCCGA GTCCGATTCC GGATCACCGA AGGCGGAAAC GCGCACCGTT 
TCGGGCTCAA AAACGGGCCA AAAGAAGTCC TCGAACAAGC AGAACACGAA AAAGGCGTCG 
GGCCTGAAAT CAGGAAACTA CAATCAGCCA GCGGGGGCCA GCAAGGCGGG AGCCTCCAGC 
GCTGGTGCAG TGGAAAAGCT GCAAAAGGCC GAGGGCAGGC TCTCCGGACT GGCATCCTTG 
AAGCGCGTGG GAAATGTCAA GGTGATCAGC GATGCCGAGG GACGCAACTC GACGATCAAG 
GCCAAGTTCA CGCTGGGTCC CTTGGTTCTG CGGGTGGAGA AGTCCTTTAA GAAGGGCAGC 
GTGAGGAGCG TGAAGAGTGC GACCGCCAGG ACGAACGAGA TGATGGGTCG CATCAAGTTC 
AGCGTGCTCG ATGATCGGGC CACCCTGATG TCCATCAAGG TGCAGCAGCC CAAGCAGGTG 
GAGGTGGAGA GCAAGGACAA TCACGACCGC ACCCGCGAAT TTGTGTGGCG ACGCACGCCC 
AAAATTGCCA AATTGGTCAA CGAGAAACTG AAATTGGCAG CCGAATCCTT GTTTGCCCCA 
CAGGGCGTCG AGGTTGTCAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect ( NM_079967.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524706.2 Drosophila melanogaster ectodermal (ect), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20465
Clone ID Related Accession (Same CDS sequence)	NM_079967.6 , NM_168400.3
Accession Version	NM_079967.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ectodermal, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079967.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTAT CGCCGGTGAC 
GACGACGAGG AGGCCGACGA TGATGATGAC GATGACGACG ATGACATTAT CGGCGATGAC 
ATCATCGAGG CCCGCCGTGA AGCACGTGAC CTGAAGAACA ACGTCATCGA TATGTCCACG 
GATGCTTTCC AGGCCCGCCA CAACCACTTC ATCGATGCCA TCTTCTCGAG GATCAATCGC 
ATTGTTTCGG AAAACTATGA TCCCTATGTG GTCAATCTAA CGGGTCGCTC CGTTTTGCCA 
AATGCCGCCA GTGGGTCAAA TGTGAAGGCC GCTAACAAGA AGCAATCGAC GAGCAGCAAG 
GTCGGAGGTC ACAAGAAGCT GAAGAACACG CGCTCGGAGC CCCGTTCCGT CAATTCGAAC 
CAGGAGGCAC GGGAGGCTGC GGTGAAGCTG CAGGAGCAAT TGACGCAATT ACAGTCCGAG 
CTGGCCATCA AAGCCGTGGA GAAGAGGCCC AGCCCCACAT CGCCTGCCAC CGAGTCCGAG 
TCCGATTCCG GATCACCGAA GGCGGAAACG CGCACCGTTT CGGGCTCAAA AACGGGCCAA 
AAGAAGTCCT CGAACAAGCA GAACACGAAA AAGGCGTCGG GCCTGAAATC AGGAAACTAC 
AATCAGCCAG CGGGGGCCAG CAAGGCGGGA GCCTCCAGCG CTGGTGCAGT GGAAAAGCTG 
CAAAAGGCCG AGGGCAGGCT CTCCGGACTG GCATCCTTGA AGCGCGTGGG AAATGTCAAG 
GTGATCAGCG ATGCCGAGGG ACGCAACTCG ACGATCAAGG CCAAGTTCAC GCTGGGTCCC 
TTGGTTCTGC GGGTGGAGAA GTCCTTTAAG AAGGGCAGCG TGAGGAGCGT GAAGAGTGCG 
ACCGCCAGGA CGAACGAGAT GATGGGTCGC ATCAAGTTCA GCGTGCTCGA TGATCGGGCC 
ACCCTGATGT CCATCAAGGT GCAGCAGCCC AAGCAGGTGG AGGTGGAGAG CAAGGACAAT 
CACGACCGCA CCCGCGAATT TGTGTGGCGA CGCACGCCCA AAATTGCCAA ATTGGTCAAC 
GAGAAACTGA AATTGGCAGC CGAATCCTTG TTTGCCCCAC AGGGCGTCGA GGTTGTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524706.2
CDS	191..1936
Translation	MKFVILLCVLSALLLQIEASPLQRLSRSERAVSRQQTVNNEVAPAVAPASDDDDDDDDEDDDDEPDLGDLIDDDDDDDEEEDDDEDEEDDTPQGQAAPAATASDDDEEEDDDDDDYLDRLFDDILGDDEDEDDDDETPAATAQQSAVAAAPAQTLDEVAPAAASSVSAGISDGVDLPAESGNAAEVLAAGNAADESVSAAVAPAQNAAASSSNNEGIAGDDDEEADDDDDDDDDDIIGDDIIEARREARDLKNNVIDMSTDAFQARHNHFIDAIFSRINRIVSENYDPYVVNLTGRSVLPNAASGSNVKAANKKQSTSSKVGGHKKLKNTRSEPRSVNSNQEAREAAVKLQEQLTQLQSELAIKAVEKRPSPTSPATESESDSGSPKAETRTVSGSKTGQKKSSNKQNTKKASGLKSGNYNQPAGASKAGASSAGAVEKLQKAEGRLSGLASLKRVGNVKVISDAEGRNSTIKAKFTLGPLVLRVEKSFKKGSVRSVKSATARTNEMMGRIKFSVLDDRATLMSIKVQQPKQVEVESKDNHDRTREFVWRRTPKIAKLVNEKLKLAAESLFAPQGVEVVRL

Target ORF information:

RefSeq Version	NM_079967.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ectodermal (ect), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079967.6

ORF Insert Sequence:

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTAT CGCCGGTGAC 
GACGACGAGG AGGCCGACGA TGATGATGAC GATGACGACG ATGACATTAT CGGCGATGAC 
ATCATCGAGG CCCGCCGTGA AGCACGTGAC CTGAAGAACA ACGTCATCGA TATGTCCACG 
GATGCTTTCC AGGCCCGCCA CAACCACTTC ATCGATGCCA TCTTCTCGAG GATCAATCGC 
ATTGTTTCGG AAAACTATGA TCCCTATGTG GTCAATCTAA CGGGTCGCTC CGTTTTGCCA 
AATGCCGCCA GTGGGTCAAA TGTGAAGGCC GCTAACAAGA AGCAATCGAC GAGCAGCAAG 
GTCGGAGGTC ACAAGAAGCT GAAGAACACG CGCTCGGAGC CCCGTTCCGT CAATTCGAAC 
CAGGAGGCAC GGGAGGCTGC GGTGAAGCTG CAGGAGCAAT TGACGCAATT ACAGTCCGAG 
CTGGCCATCA AAGCCGTGGA GAAGAGGCCC AGCCCCACAT CGCCTGCCAC CGAGTCCGAG 
TCCGATTCCG GATCACCGAA GGCGGAAACG CGCACCGTTT CGGGCTCAAA AACGGGCCAA 
AAGAAGTCCT CGAACAAGCA GAACACGAAA AAGGCGTCGG GCCTGAAATC AGGAAACTAC 
AATCAGCCAG CGGGGGCCAG CAAGGCGGGA GCCTCCAGCG CTGGTGCAGT GGAAAAGCTG 
CAAAAGGCCG AGGGCAGGCT CTCCGGACTG GCATCCTTGA AGCGCGTGGG AAATGTCAAG 
GTGATCAGCG ATGCCGAGGG ACGCAACTCG ACGATCAAGG CCAAGTTCAC GCTGGGTCCC 
TTGGTTCTGC GGGTGGAGAA GTCCTTTAAG AAGGGCAGCG TGAGGAGCGT GAAGAGTGCG 
ACCGCCAGGA CGAACGAGAT GATGGGTCGC ATCAAGTTCA GCGTGCTCGA TGATCGGGCC 
ACCCTGATGT CCATCAAGGT GCAGCAGCCC AAGCAGGTGG AGGTGGAGAG CAAGGACAAT 
CACGACCGCA CCCGCGAATT TGTGTGGCGA CGCACGCCCA AAATTGCCAA ATTGGTCAAC 
GAGAAACTGA AATTGGCAGC CGAATCCTTG TTTGCCCCAC AGGGCGTCGA GGTTGTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect ( NM_168400.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729596.1 Drosophila melanogaster ectodermal (ect), transcript variant B, mRNA.

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CloneID	OFa20465
Clone ID Related Accession (Same CDS sequence)	NM_079967.6 , NM_168400.3
Accession Version	NM_168400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ectodermal, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168400.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTAT CGCCGGTGAC 
GACGACGAGG AGGCCGACGA TGATGATGAC GATGACGACG ATGACATTAT CGGCGATGAC 
ATCATCGAGG CCCGCCGTGA AGCACGTGAC CTGAAGAACA ACGTCATCGA TATGTCCACG 
GATGCTTTCC AGGCCCGCCA CAACCACTTC ATCGATGCCA TCTTCTCGAG GATCAATCGC 
ATTGTTTCGG AAAACTATGA TCCCTATGTG GTCAATCTAA CGGGTCGCTC CGTTTTGCCA 
AATGCCGCCA GTGGGTCAAA TGTGAAGGCC GCTAACAAGA AGCAATCGAC GAGCAGCAAG 
GTCGGAGGTC ACAAGAAGCT GAAGAACACG CGCTCGGAGC CCCGTTCCGT CAATTCGAAC 
CAGGAGGCAC GGGAGGCTGC GGTGAAGCTG CAGGAGCAAT TGACGCAATT ACAGTCCGAG 
CTGGCCATCA AAGCCGTGGA GAAGAGGCCC AGCCCCACAT CGCCTGCCAC CGAGTCCGAG 
TCCGATTCCG GATCACCGAA GGCGGAAACG CGCACCGTTT CGGGCTCAAA AACGGGCCAA 
AAGAAGTCCT CGAACAAGCA GAACACGAAA AAGGCGTCGG GCCTGAAATC AGGAAACTAC 
AATCAGCCAG CGGGGGCCAG CAAGGCGGGA GCCTCCAGCG CTGGTGCAGT GGAAAAGCTG 
CAAAAGGCCG AGGGCAGGCT CTCCGGACTG GCATCCTTGA AGCGCGTGGG AAATGTCAAG 
GTGATCAGCG ATGCCGAGGG ACGCAACTCG ACGATCAAGG CCAAGTTCAC GCTGGGTCCC 
TTGGTTCTGC GGGTGGAGAA GTCCTTTAAG AAGGGCAGCG TGAGGAGCGT GAAGAGTGCG 
ACCGCCAGGA CGAACGAGAT GATGGGTCGC ATCAAGTTCA GCGTGCTCGA TGATCGGGCC 
ACCCTGATGT CCATCAAGGT GCAGCAGCCC AAGCAGGTGG AGGTGGAGAG CAAGGACAAT 
CACGACCGCA CCCGCGAATT TGTGTGGCGA CGCACGCCCA AAATTGCCAA ATTGGTCAAC 
GAGAAACTGA AATTGGCAGC CGAATCCTTG TTTGCCCCAC AGGGCGTCGA GGTTGTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729596.1
CDS	134..1879
Translation	MKFVILLCVLSALLLQIEASPLQRLSRSERAVSRQQTVNNEVAPAVAPASDDDDDDDDEDDDDEPDLGDLIDDDDDDDEEEDDDEDEEDDTPQGQAAPAATASDDDEEEDDDDDDYLDRLFDDILGDDEDEDDDDETPAATAQQSAVAAAPAQTLDEVAPAAASSVSAGISDGVDLPAESGNAAEVLAAGNAADESVSAAVAPAQNAAASSSNNEGIAGDDDEEADDDDDDDDDDIIGDDIIEARREARDLKNNVIDMSTDAFQARHNHFIDAIFSRINRIVSENYDPYVVNLTGRSVLPNAASGSNVKAANKKQSTSSKVGGHKKLKNTRSEPRSVNSNQEAREAAVKLQEQLTQLQSELAIKAVEKRPSPTSPATESESDSGSPKAETRTVSGSKTGQKKSSNKQNTKKASGLKSGNYNQPAGASKAGASSAGAVEKLQKAEGRLSGLASLKRVGNVKVISDAEGRNSTIKAKFTLGPLVLRVEKSFKKGSVRSVKSATARTNEMMGRIKFSVLDDRATLMSIKVQQPKQVEVESKDNHDRTREFVWRRTPKIAKLVNEKLKLAAESLFAPQGVEVVRL

Target ORF information:

RefSeq Version	NM_168400.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ectodermal (ect), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168400.3

ORF Insert Sequence:

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGATGA TGATGAGGAG 
GAGGACGATG ACGAGGACGA GGAGGATGAT ACGCCCCAGG GTCAGGCTGC TCCAGCGGCC 
ACTGCCAGCG ATGATGACGA AGAGGAAGAC GATGATGATG ACGATTATCT GGACAGGCTG 
TTCGATGATA TCCTGGGCGA TGATGAAGAT GAGGATGATG ATGACGAGAC ACCCGCTGCC 
ACCGCCCAGC AGAGCGCTGT GGCCGCCGCT CCCGCCCAAA CTCTGGATGA GGTCGCCCCC 
GCTGCCGCCT CCTCCGTGAG CGCCGGTATC TCCGATGGTG TTGATCTGCC CGCCGAGTCC 
GGAAATGCCG CTGAGGTCCT GGCCGCTGGA AATGCTGCCG ATGAGTCCGT TTCCGCTGCC 
GTTGCTCCGG CGCAAAACGC TGCCGCCAGC AGCAGCAACA ACGAAGGTAT CGCCGGTGAC 
GACGACGAGG AGGCCGACGA TGATGATGAC GATGACGACG ATGACATTAT CGGCGATGAC 
ATCATCGAGG CCCGCCGTGA AGCACGTGAC CTGAAGAACA ACGTCATCGA TATGTCCACG 
GATGCTTTCC AGGCCCGCCA CAACCACTTC ATCGATGCCA TCTTCTCGAG GATCAATCGC 
ATTGTTTCGG AAAACTATGA TCCCTATGTG GTCAATCTAA CGGGTCGCTC CGTTTTGCCA 
AATGCCGCCA GTGGGTCAAA TGTGAAGGCC GCTAACAAGA AGCAATCGAC GAGCAGCAAG 
GTCGGAGGTC ACAAGAAGCT GAAGAACACG CGCTCGGAGC CCCGTTCCGT CAATTCGAAC 
CAGGAGGCAC GGGAGGCTGC GGTGAAGCTG CAGGAGCAAT TGACGCAATT ACAGTCCGAG 
CTGGCCATCA AAGCCGTGGA GAAGAGGCCC AGCCCCACAT CGCCTGCCAC CGAGTCCGAG 
TCCGATTCCG GATCACCGAA GGCGGAAACG CGCACCGTTT CGGGCTCAAA AACGGGCCAA 
AAGAAGTCCT CGAACAAGCA GAACACGAAA AAGGCGTCGG GCCTGAAATC AGGAAACTAC 
AATCAGCCAG CGGGGGCCAG CAAGGCGGGA GCCTCCAGCG CTGGTGCAGT GGAAAAGCTG 
CAAAAGGCCG AGGGCAGGCT CTCCGGACTG GCATCCTTGA AGCGCGTGGG AAATGTCAAG 
GTGATCAGCG ATGCCGAGGG ACGCAACTCG ACGATCAAGG CCAAGTTCAC GCTGGGTCCC 
TTGGTTCTGC GGGTGGAGAA GTCCTTTAAG AAGGGCAGCG TGAGGAGCGT GAAGAGTGCG 
ACCGCCAGGA CGAACGAGAT GATGGGTCGC ATCAAGTTCA GCGTGCTCGA TGATCGGGCC 
ACCCTGATGT CCATCAAGGT GCAGCAGCCC AAGCAGGTGG AGGTGGAGAG CAAGGACAAT 
CACGACCGCA CCCGCGAATT TGTGTGGCGA CGCACGCCCA AAATTGCCAA ATTGGTCAAC 
GAGAAACTGA AATTGGCAGC CGAATCCTTG TTTGCCCCAC AGGGCGTCGA GGTTGTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect ( NM_001300090.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287019.1 Drosophila melanogaster ectodermal (ect), transcript variant F, mRNA.

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CloneID	OFa22208
Clone ID Related Accession (Same CDS sequence)	NM_001300090.1
Accession Version	NM_001300090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ectodermal, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGTTCT TTTGATTTCG 
GGACTTTCAG ATGATGATGA GGAGGAGGAC GATGACGAGG ACGAGGAGGA TGATACGCCC 
CAGGGTCAGG CTGCTCCAGC GGCCACTGCC AGCGATGATG ACGAAGAGGA AGACGATGAT 
GATGACGATT ATCTGGACAG GCTGTTCGAT GATATCCTGG GCGATGATGA AGATGAGGAT 
GATGATGACG AGACACCCGC TGCCACCGCC CAGCAGAGCG CTGTGGCCGC CGCTCCCGCC 
CAAACTCTGG ATGAGGTCGC CCCCGCTGCC GCCTCCTCCG TGAGCGCCGG TATCTCCGAT 
GGTGTTGATC TGCCCGCCGA GTCCGGAAAT GCCGCTGAGG TCCTGGCCGC TGGAAATGCT 
GCCGATGAGT CCGTTTCCGC TGCCGTTGCT CCGGCGCAAA ACGCTGCCGC CAGCAGCAGC 
AACAACGAAG GTATCGCCGG TGACGACGAC GAGGAGGCCG ACGATGATGA TGACGATGAC 
GACGATGACA TTATCGGCGA TGACATCATC GAGGCCCGCC GTGAAGCACG TGACCTGAAG 
AACAACGTCA TCGATATGTC CACGGATGCT TTCCAGGCCC GCCACAACCA CTTCATCGAT 
GCCATCTTCT CGAGGATCAA TCGCATTGTT TCGGAAAACT ATGATCCCTA TGTGGTCAAT 
CTAACGGGTC GCTCCGTTTT GCCAAATGCC GCCAGTGGGT CAAATGTGAA GGCCGCTAAC 
AAGAAGCAAT CGACGAGCAG CAAGGTCGGA GGTCACAAGA AGCTGAAGAA CACGCGCTCG 
GAGCCCCGTT CCGTCAATTC GAACCAGGAG GCACGGGAGG CTGCGGTGAA GCTGCAGGAG 
CAATTGACGC AATTACAGTC CGAGCTGGCC ATCAAAGCCG TGGAGAAGAG GCCCAGCCCC 
ACATCGCCTG CCACCGAGTC CGAGTCCGAT TCCGGATCAC CGAAGGCGGA AACGCGCACC 
GTTTCGGGCT CAAAAACGGG CCAAAAGAAG TCCTCGAACA AGCAGAACAC GAAAAAGGCG 
TCGGGCCTGA AATCAGGAAA CTACAATCAG CCAGCGGGGG CCAGCAAGGC GGGAGCCTCC 
AGCGCTGGTG CAGTGGAAAA GCTGCAAAAG GCCGAGGGCA GGCTCTCCGG ACTGGCATCC 
TTGAAGCGCG TGGGAAATGT CAAGGTGATC AGCGATGCCG AGGGACGCAA CTCGACGATC 
AAGGCCAAGT TCACGCTGGG TCCCTTGGTT CTGCGGGTGG AGAAGTCCTT TAAGAAGGGC 
AGCGTGAGGA GCGTGAAGAG TGCGACCGCC AGGACGAACG AGATGATGGG TCGCATCAAG 
TTCAGCGTGC TCGATGATCG GGCCACCCTG ATGTCCATCA AGGTGCAGCA GCCCAAGCAG 
GTGGAGGTGG AGAGCAAGGA CAATCACGAC CGCACCCGCG AATTTGTGTG GCGACGCACG 
CCCAAAATTG CCAAATTGGT CAACGAGAAA CTGAAATTGG CAGCCGAATC CTTGTTTGCC 
CCACAGGGCG TCGAGGTTGT CAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287019.1
CDS	134..1903
Translation	MKFVILLCVLSALLLQIEASPLQRLSRSERAVSRQQTVNNEVAPAVAPASDDDDDDDDEDDDDEPDLGDLIDDDDVLLISGLSDDDEEEDDDEDEEDDTPQGQAAPAATASDDDEEEDDDDDDYLDRLFDDILGDDEDEDDDDETPAATAQQSAVAAAPAQTLDEVAPAAASSVSAGISDGVDLPAESGNAAEVLAAGNAADESVSAAVAPAQNAAASSSNNEGIAGDDDEEADDDDDDDDDDIIGDDIIEARREARDLKNNVIDMSTDAFQARHNHFIDAIFSRINRIVSENYDPYVVNLTGRSVLPNAASGSNVKAANKKQSTSSKVGGHKKLKNTRSEPRSVNSNQEAREAAVKLQEQLTQLQSELAIKAVEKRPSPTSPATESESDSGSPKAETRTVSGSKTGQKKSSNKQNTKKASGLKSGNYNQPAGASKAGASSAGAVEKLQKAEGRLSGLASLKRVGNVKVISDAEGRNSTIKAKFTLGPLVLRVEKSFKKGSVRSVKSATARTNEMMGRIKFSVLDDRATLMSIKVQQPKQVEVESKDNHDRTREFVWRRTPKIAKLVNEKLKLAAESLFAPQGVEVVRL

Target ORF information:

RefSeq Version	NM_001300090.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ectodermal (ect), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300090.1

ORF Insert Sequence:

ATGAAGTTCG TGATTTTGCT GTGCGTGCTG AGCGCGTTGC TGCTGCAAAT TGAAGCGTCG 
CCCCTCCAGC GGCTTTCGAG AAGCGAGCGG GCTGTTTCCC GCCAACAGAC GGTCAACAAT 
GAGGTGGCTC CGGCGGTAGC TCCTGCCAGC GATGATGACG ATGATGACGA CGATGAGGAC 
GACGATGATG AGCCTGATCT AGGAGATCTG ATCGATGACG ATGATGTTCT TTTGATTTCG 
GGACTTTCAG ATGATGATGA GGAGGAGGAC GATGACGAGG ACGAGGAGGA TGATACGCCC 
CAGGGTCAGG CTGCTCCAGC GGCCACTGCC AGCGATGATG ACGAAGAGGA AGACGATGAT 
GATGACGATT ATCTGGACAG GCTGTTCGAT GATATCCTGG GCGATGATGA AGATGAGGAT 
GATGATGACG AGACACCCGC TGCCACCGCC CAGCAGAGCG CTGTGGCCGC CGCTCCCGCC 
CAAACTCTGG ATGAGGTCGC CCCCGCTGCC GCCTCCTCCG TGAGCGCCGG TATCTCCGAT 
GGTGTTGATC TGCCCGCCGA GTCCGGAAAT GCCGCTGAGG TCCTGGCCGC TGGAAATGCT 
GCCGATGAGT CCGTTTCCGC TGCCGTTGCT CCGGCGCAAA ACGCTGCCGC CAGCAGCAGC 
AACAACGAAG GTATCGCCGG TGACGACGAC GAGGAGGCCG ACGATGATGA TGACGATGAC 
GACGATGACA TTATCGGCGA TGACATCATC GAGGCCCGCC GTGAAGCACG TGACCTGAAG 
AACAACGTCA TCGATATGTC CACGGATGCT TTCCAGGCCC GCCACAACCA CTTCATCGAT 
GCCATCTTCT CGAGGATCAA TCGCATTGTT TCGGAAAACT ATGATCCCTA TGTGGTCAAT 
CTAACGGGTC GCTCCGTTTT GCCAAATGCC GCCAGTGGGT CAAATGTGAA GGCCGCTAAC 
AAGAAGCAAT CGACGAGCAG CAAGGTCGGA GGTCACAAGA AGCTGAAGAA CACGCGCTCG 
GAGCCCCGTT CCGTCAATTC GAACCAGGAG GCACGGGAGG CTGCGGTGAA GCTGCAGGAG 
CAATTGACGC AATTACAGTC CGAGCTGGCC ATCAAAGCCG TGGAGAAGAG GCCCAGCCCC 
ACATCGCCTG CCACCGAGTC CGAGTCCGAT TCCGGATCAC CGAAGGCGGA AACGCGCACC 
GTTTCGGGCT CAAAAACGGG CCAAAAGAAG TCCTCGAACA AGCAGAACAC GAAAAAGGCG 
TCGGGCCTGA AATCAGGAAA CTACAATCAG CCAGCGGGGG CCAGCAAGGC GGGAGCCTCC 
AGCGCTGGTG CAGTGGAAAA GCTGCAAAAG GCCGAGGGCA GGCTCTCCGG ACTGGCATCC 
TTGAAGCGCG TGGGAAATGT CAAGGTGATC AGCGATGCCG AGGGACGCAA CTCGACGATC 
AAGGCCAAGT TCACGCTGGG TCCCTTGGTT CTGCGGGTGG AGAAGTCCTT TAAGAAGGGC 
AGCGTGAGGA GCGTGAAGAG TGCGACCGCC AGGACGAACG AGATGATGGG TCGCATCAAG 
TTCAGCGTGC TCGATGATCG GGCCACCCTG ATGTCCATCA AGGTGCAGCA GCCCAAGCAG 
GTGGAGGTGG AGAGCAAGGA CAATCACGAC CGCACCCGCG AATTTGTGTG GCGACGCACG 
CCCAAAATTG CCAAATTGGT CAACGAGAAA CTGAAATTGG CAGCCGAATC CTTGTTTGCC 
CCACAGGGCG TCGAGGTTGT CAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ect cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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