Nct cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nct
Entrez Gene ID	42964
Full Name	nicastrin
Synonyms	CG7012,Dmel\CG7012,Nct,Nic,S5A,ST96B,agro,dNct,nic
General protein information	Preferred Names nicastrin Names CG7012 gene product from transcript CG7012-RC CG7012-PA CG7012-PC CG7012-PD CG7012-PE agoraphobic dNicastrin nct-PA nct-PC nct-PD nct-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96B1-96B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_206568.4	NP_996291.2	nicastrin, isoform D
NM_001276003.1	NP_001262932.1	nicastrin, isoform E
NM_001170242.2	NP_001163713.1	nicastrin, isoform C
NM_143040.5	NP_651297.2	nicastrin, isoform A

Pathways from BioSystems

Homologies

	NCSTN	XP_001117549.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001734	XP_321352.4
Caenorhabditis elegans (roundworm)	aph-2	NP_492712.2
Arabidopsis thaliana (thale cress)	AT3G52640	NP_974419.1
Homo sapiens (human)	NCSTN	NP_056146.1
Danio rerio (zebrafish)	ncstn	NP_001009556.1
Pan troglodytes (chimpanzee)	NCSTN	XP_003949661.1
Rattus norvegicus (Norway rat)	Ncstn	NP_777353.1
Drosophila melanogaster (fruit fly)	nct	NP_001262932.1
Bos taurus (cattle)	NCSTN	NP_001029647.1
Mus musculus (house mouse)	Ncstn	NP_067620.3
Canis lupus familiaris (dog)	NCSTN	XP_005640953.1
Gallus gallus (chicken)	NCSTN	NP_001004413.2
Oryza sativa (rice)	Os02g0736500	NP_001048054.1
Xenopus tropicalis (tropical clawed frog)	ncstn	NP_001123711.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nct is required for neuronal survival during aging and normal lifespan.
Title: An Evolutionarily Conserved Role of Presenilin in Neuronal Protection in the Aging

Down-regulation of the ATP-binding cassette transporter 2 (Abca2) reduces amyloid-beta production by altering Nicastrin maturation and intracellular localization.
Title: Down-regulation of the ATP-binding cassette transporter 2 (Abca2) reduces amyloid-? production by altering Nicastrin maturation and intracellular localization.

Nicastrin is essential to early photoreceptor neuron development
Title: Nicastrin controls aspects of photoreceptor neuron specification and differentiation in the Drosophila eye.

presenilin, nicastrin, APH-1, and PEN-2, are present and enriched on phagosome membranes from both murine macrophages and Drosophila S2 phagocytes
Title: Gamma-secretase is a functional component of phagosomes.

the proper assembly of the Aph-1.nicastrin subcomplex with presenilin is the prerequisite for the trafficking as well as the enzymatic activity of the gamma-secretase complex and Aph-1 functions as a stabilizing scaffold in the assembly of this complex
Title: Aph-1 contributes to the stabilization and trafficking of the gamma-secretase complex through mechanisms involving intermolecular and intramolecular interactions.

The following Nct gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nct cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06030	NM_206568.4 Latest version!	Drosophila melanogaster nicastrin, transcript variant D (Nct), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06031	NM_001170242.2 Latest version!	Drosophila melanogaster nicastrin, transcript variant C (Nct), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06031	NM_143040.5 Latest version!	Drosophila melanogaster nicastrin, transcript variant A (Nct), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa07742	NM_001276003.1 Latest version!	Drosophila melanogaster nicastrin, transcript variant E (Nct), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nct ( NM_206568.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996291.2 Drosophila melanogaster nicastrin, transcript variant D (Nct), mRNA.

Price & Availability↑

CloneID	OFa06030
Clone ID Related Accession (Same CDS sequence)	NM_206568.4
Accession Version	NM_206568.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicastrin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_206568.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996291.2
CDS	49..2127
Translation	MEMRLNAASIWLLILSYGATIAQGERTRDKMYEPIGGASCFRRLNGTHQTGCSSTYSGSVGVLHLINVEADLEFLLSSPPSPPYAPMIPPHLFTRNNLMRLKEAGPKNISVVLLINRTNQMKQFSHELNCPNQYSGLNSTSETCDASNPAKNWNPWGTGLLHEDFPFPIYYIADLDQVTKLEKCFQDFNNHNYETHALRSLCAVEVKSFMSAAVNTEVCMRRTNFINNLGGSKYCDPLEGRNVYATLYPRKPAIENNLETVHTNEKFILVTCRLDTTTMFDGVGLGAMDSLMGFAVFTHVAYLLKQLLPPQSKDLHNVLFVTFNGESYDYIGSQRFVYDMEKLQFPTESTGTPPIAFDNIDFMLDIGTLDDISNIKLHALNGTTLAQQILERLNNYAKSPRYGFNLNIQSEMSAHLPPTSAQSFLRRDPNFNALILNARPTNKYYHSIYDDADNVDFTYANTSKDFTQLTEVNDFKSLNPDSLQMKVRNVSSIVAMALYQTITGKEYTGTKVANPLMADEFLYCFLQSADCPLFKAASYPGSQLTNLPPMRYISVLGGSQESSGYTYRLLGYLLSQLQPDIHRDNCTDLPLHYFAGFNNIGECRLTTQNYSHALSPAFLIDGYDWSSGMYSTWTESTWSQFSARIFLRPSNVHQVTTLSVGIVVLIISFCLVYIISSRSEVLFEDLPASNAA

Target ORF information:

RefSeq Version	NM_206568.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicastrin, transcript variant D (Nct), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206568.4

ORF Insert Sequence:

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nct ( NM_001170242.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163713.1 Drosophila melanogaster nicastrin, transcript variant C (Nct), mRNA.

Price & Availability↑

CloneID	OFa06031
Clone ID Related Accession (Same CDS sequence)	NM_001170242.2 , NM_143040.5
Accession Version	NM_001170242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicastrin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170242.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCATTAT TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163713.1
CDS	49..2136
Translation	MEMRLNAASIWLLILSYGATIAQGERTRDKMYEPIGGASCFRRLNGTHQTGCSSTYSGSVGVLHLINVEADLEFLLSSPPSPPYAPMIPPHLFTRNNLMRLKEAGPKNISVVLLINRTNQMKQFSHELNCPNQYSGLNSTSETCDASNPAKNWNPWGTGLLHEDFPFPIYYIADLDQVTKLEKCFQDFNNHNYETHALRSLCAVEVKSFMSAAVNTEVCMRRTNFINNLGGSKYCDPLEGRNVYATLYPRKPAIENNLETVHTNEKFILVTCRLDTTTMFDGVGLGAMDSLMGFAVFTHVAYLLKQLLPPQSKDLHNVLFVTFNGESYDYIGSQRFVYDMEKLQFPTESTGTPPIAFDNIDFMLDIGTLDDISNIKLHALNGTTLAQQILERLNNYAKSPRYGFNLNIQSEMSAHLPPTSAQSFLRRDPNFNALILNARPTNKYYHSIYDDADNVDFTYANTSKDFTQLTEVNDFKSLNPDSLQMKVRNVSSIVAMALYQTITGKEYTGTKVANPLMADEFLYCFLQSADCPLFKAASYPGSQLTNLPPMRYISVLGGSQESSGYTYRLLGYLLSQLQPDIHRDNCTDLPLHYFAGFNNIGECRLTTQNYSHALSPAFLIDGYDWSSGMYSTWTESTWSQFSARIFLRPSNVHQVTTLSVGIVVLIISFCLVYIISSRSEVLFEDLPASNAALFG

Target ORF information:

RefSeq Version	NM_001170242.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicastrin, transcript variant C (Nct), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170242.2

ORF Insert Sequence:

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCATTAT TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nct ( NM_143040.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651297.2 Drosophila melanogaster nicastrin, transcript variant A (Nct), mRNA.

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CloneID	OFa06031
Clone ID Related Accession (Same CDS sequence)	NM_001170242.2 , NM_143040.5
Accession Version	NM_143040.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicastrin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143040.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCATTAT TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651297.2
CDS	49..2136
Translation	MEMRLNAASIWLLILSYGATIAQGERTRDKMYEPIGGASCFRRLNGTHQTGCSSTYSGSVGVLHLINVEADLEFLLSSPPSPPYAPMIPPHLFTRNNLMRLKEAGPKNISVVLLINRTNQMKQFSHELNCPNQYSGLNSTSETCDASNPAKNWNPWGTGLLHEDFPFPIYYIADLDQVTKLEKCFQDFNNHNYETHALRSLCAVEVKSFMSAAVNTEVCMRRTNFINNLGGSKYCDPLEGRNVYATLYPRKPAIENNLETVHTNEKFILVTCRLDTTTMFDGVGLGAMDSLMGFAVFTHVAYLLKQLLPPQSKDLHNVLFVTFNGESYDYIGSQRFVYDMEKLQFPTESTGTPPIAFDNIDFMLDIGTLDDISNIKLHALNGTTLAQQILERLNNYAKSPRYGFNLNIQSEMSAHLPPTSAQSFLRRDPNFNALILNARPTNKYYHSIYDDADNVDFTYANTSKDFTQLTEVNDFKSLNPDSLQMKVRNVSSIVAMALYQTITGKEYTGTKVANPLMADEFLYCFLQSADCPLFKAASYPGSQLTNLPPMRYISVLGGSQESSGYTYRLLGYLLSQLQPDIHRDNCTDLPLHYFAGFNNIGECRLTTQNYSHALSPAFLIDGYDWSSGMYSTWTESTWSQFSARIFLRPSNVHQVTTLSVGIVVLIISFCLVYIISSRSEVLFEDLPASNAALFG

Target ORF information:

RefSeq Version	NM_143040.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicastrin, transcript variant A (Nct), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143040.5

ORF Insert Sequence:

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCATTAT TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nct ( NM_001276003.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262932.1 Drosophila melanogaster nicastrin, transcript variant E (Nct), mRNA.

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CloneID	OFa07742
Clone ID Related Accession (Same CDS sequence)	NM_001276003.1
Accession Version	NM_001276003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicastrin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCATCGC CGCGGCCGAC AGCCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262932.1
CDS	49..2148
Translation	MEMRLNAASIWLLILSYGATIAQGERTRDKMYEPIGGASCFRRLNGTHQTGCSSTYSGSVGVLHLINVEADLEFLLSSPPSPPYAPMIPPHLFTRNNLMRLKEAGPKNISVVLLINRTNQMKQFSHELNCPNQYSGLNSTSETCDASNPAKNWNPWGTGLLHEDFPFPIYYIADLDQVTKLEKCFQDFNNHNYETHALRSLCAVEVKSFMSAAVNTEVCMRRTNFINNLGGSKYCDPLEGRNVYATLYPRKPAIENNLETVHTNEKFILVTCRLDTTTMFDGVGLGAMDSLMGFAVFTHVAYLLKQLLPPQSKDLHNVLFVTFNGESYDYIGSQRFVYDMEKLQFPTESTGTPPIAFDNIDFMLDIGTLDDISNIKLHALNGTTLAQQILERLNNYAKSPRYGFNLNIQSEMSAHLPPTSAQSFLRRDPNFNALILNARPTNKYYHSIYDDADNVDFTYANTSKDFTQLTEVNDFKSLNPDSLQMKVRNVSSIVAMALYQTITGKEYTGTKVANPLMADEFLYCFLQSADCPLFKAASYPGSQLTNLPPMRYISVLGGSQESSGYTYRLLGYLLSQLQPDIHRDNCTDLPLHYFAGFNNIGECRLTTQNYSHALSPAFLIDGYDWSSGMYSTWTESTWSQFSARIFLRPSNVHQVTTLSVGIVVLIISFCLVYIISSRSEVLFEDLPASNAASPRPTAC

Target ORF information:

RefSeq Version	NM_001276003.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicastrin, transcript variant E (Nct), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276003.1

ORF Insert Sequence:

ATGGAAATGC GTCTGAATGC GGCTTCCATA TGGCTGTTAA TACTGTCGTA TGGAGCAACT 
ATTGCTCAAG GAGAAAGAAC CCGCGATAAG ATGTACGAGC CCATTGGAGG AGCTAGCTGT 
TTCCGACGGC TGAATGGCAC CCATCAGACA GGCTGTTCCT CAACCTACTC CGGTTCCGTG 
GGCGTACTAC ATCTAATAAA CGTCGAGGCC GACCTGGAAT TTCTTCTTAG CAGCCCACCA 
TCTCCACCTT ACGCCCCCAT GATACCACCT CACCTGTTCA CACGTAACAA CCTGATGCGC 
CTAAAGGAAG CCGGACCAAA GAACATTTCT GTGGTGCTGC TGATCAACCG CACGAACCAG 
ATGAAGCAGT TCTCGCACGA ACTCAACTGC CCCAATCAGT ACAGCGGCCT GAACAGCACC 
AGTGAGACCT GCGACGCCAG CAATCCAGCC AAAAACTGGA ATCCCTGGGG CACTGGACTT 
CTGCACGAGG ACTTTCCCTT TCCTATCTAT TACATAGCCG ATTTGGATCA GGTCACCAAG 
CTAGAGAAGT GCTTTCAGGA CTTTAACAAC CATAACTACG AGACGCACGC GCTGCGTAGC 
TTGTGCGCCG TCGAGGTCAA GTCCTTTATG TCCGCCGCTG TCAACACCGA GGTCTGTATG 
CGCCGCACCA ACTTCATCAA TAATCTTGGA GGAAGCAAGT ACTGCGATCC GCTCGAGGGA 
CGGAATGTTT ACGCCACCTT GTACCCCCGA AAGCCAGCAA TCGAAAACAA CTTGGAGACA 
GTCCATACGA ATGAAAAGTT CATATTAGTA ACCTGTCGCC TGGACACCAC CACCATGTTC 
GATGGCGTCG GTCTTGGAGC CATGGACTCC CTCATGGGAT TTGCTGTTTT CACTCATGTG 
GCGTATCTAT TAAAACAACT ACTTCCGCCG CAAAGCAAAG ACCTTCATAA TGTCCTCTTT 
GTGACTTTTA ATGGCGAATC CTATGACTAC ATTGGTTCTC AAAGATTTGT ATACGACATG 
GAGAAACTTC AATTTCCTAC TGAATCCACA GGCACGCCTC CGATTGCCTT TGACAATATT 
GACTTCATGC TGGACATCGG GACACTGGAT GACATATCGA ATATTAAGCT GCATGCGTTA 
AATGGAACGA CTTTGGCTCA GCAAATTCTA GAGCGGCTAA ATAACTATGC GAAGTCGCCA 
CGCTATGGCT TTAACCTGAA CATTCAGTCC GAGATGAGCG CTCACTTACC ACCTACGTCG 
GCGCAATCAT TTCTGCGACG TGATCCAAAC TTCAATGCAT TGATTCTAAA CGCTCGTCCA 
ACGAACAAGT ATTATCATTC CATATACGAT GACGCGGATA ACGTGGACTT CACCTATGCG 
AACACAAGCA AGGATTTCAC CCAGCTGACG GAAGTTAATG ACTTTAAAAG CTTGAACCCA 
GATTCACTGC AAATGAAAGT GCGCAACGTT TCCTCTATTG TGGCCATGGC CCTATATCAG 
ACAATAACTG GAAAGGAGTA CACTGGCACC AAGGTGGCCA ACCCTCTGAT GGCAGATGAG 
TTCCTTTACT GTTTCCTGCA ATCGGCGGAC TGCCCACTCT TTAAGGCCGC ATCTTATCCG 
GGCAGTCAGC TCACCAATTT GCCTCCGATG CGCTACATAA GCGTCTTGGG TGGCTCTCAA 
GAGTCGTCGG GCTATACGTA TAGATTGCTG GGCTATCTCC TGTCACAACT GCAGCCAGAC 
ATTCACAGAG ATAACTGCAC CGACTTGCCG CTGCACTATT TCGCCGGATT CAACAATATC 
GGAGAGTGTC GCCTCACCAC GCAGAACTAC AGTCACGCCC TGAGTCCAGC TTTTCTTATT 
GATGGCTACG ATTGGAGTTC CGGCATGTAT TCCACTTGGA CTGAATCCAC CTGGTCACAG 
TTCAGTGCAC GCATCTTCCT GCGCCCGTCC AATGTGCACC AGGTCACAAC TCTAAGCGTT 
GGCATAGTGG TGCTGATAAT ATCCTTCTGT TTGGTGTATA TAATCAGCTC ACGATCGGAA 
GTCCTCTTTG AGGATTTGCC GGCAAGCAAT GCCGCATCGC CGCGGCCGAC AGCCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nct cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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