Dlc90F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dlc90F
Entrez Gene ID	42199
Full Name	Dynein light chain 90F
Synonyms	CG12363,Dmel\CG12363,TcTex-1,Tctex,Tctex-1,Tctex1,bs35g12.y1,dlc90F,dtctex-1,l(3)04091,tctex,tctex-1
General protein information	Preferred Names Dynein light chain 90F Names CG12363 gene product from transcript CG12363-RA CG12363-PA Dlc90F-PA dynein light chain 90F dynein light chain; tctex-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91A2-91A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_058008.4	NP_477356.1	dynein light chain 90F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Dlc90F	NP_477356.1
Homo sapiens (human)	DYNLT1	NP_006510.1
Bos taurus (cattle)	DYNLT1	NP_777045.1
Mus musculus (house mouse)	Dynlt1f	NP_001160099.1
Mus musculus (house mouse)	Dynlt1a	NP_001160101.1
Danio rerio (zebrafish)	dynlt1	NP_001129255.1
Pan troglodytes (chimpanzee)	DYNLT1	XP_001147088.1
Gallus gallus (chicken)	DYNLT1	XP_001232357.1
Mus musculus (house mouse)	Dynlt1c	NP_001160102.1
Xenopus tropicalis (tropical clawed frog)	dynlt1	NP_001011428.1
Canis lupus familiaris (dog)	DYNLT1	XP_003638830.1
Mus musculus (house mouse)	Dynlt1b	NP_033368.1
Rattus norvegicus (Norway rat)	Dynlt1	NP_112608.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001229	XP_321929.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dynein light chain 1 is required for protein clearance by autophagy that is associated with autophagic cell death and neurodegeneration.
Title: Dynein light chain 1 is required for autophagy, protein clearance, and cell death in Drosophila.

x-ray crystallographic analysis of dynein light chain TcTex-1 structure
Title: Crystal structure of dynein light chain TcTex-1.

Dlc90F is required for the localization of cytoplasmic dynein and is not essential for early development or in the adult organism.
Title: The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation.

Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74
Title: Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74.

The following Dlc90F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dlc90F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08495	NM_058008.4 Latest version!	Drosophila melanogaster dynein light chain 90F (Dlc90F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dlc90F ( NM_058008.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477356.1 Drosophila melanogaster dynein light chain 90F (Dlc90F), mRNA.

Price & Availability↑

CloneID	OFa08495
Clone ID Related Accession (Same CDS sequence)	NM_058008.4
Accession Version	NM_058008.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dynein light chain 90F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_058008.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACT CACGCGAAGA AAGCCAGTTC ATTGTGGACG ACGTGAGCAA GACGATTAAA 
GAGGCCATCG AGACGACCAT CGGCGGTAAC GCCTACCAGC ACGACAAGGT GAACAACTGG 
ACCGGCCAGG TGGTGGAGAA CTGCCTGACG GTGCTCACCA AGGAGCAGAA GCCCTACAAG 
TACATTGTGA CCGCCATGAT CATGCAAAAG AACGGTGCTG GACTCCACAC CGCCAGCTCC 
TGCTACTGGA ACAACGACAC CGACGGATCG TGCACAGTAC GCTGGGAGAA CAAGACCATG 
TACTGCATCG TTTCGGTCTT CGGACTGGCC GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477356.1
CDS	194..529
Translation	MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV

Target ORF information:

RefSeq Version	NM_058008.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dynein light chain 90F (Dlc90F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058008.4

ORF Insert Sequence:

ATGGATGACT CACGCGAAGA AAGCCAGTTC ATTGTGGACG ACGTGAGCAA GACGATTAAA 
GAGGCCATCG AGACGACCAT CGGCGGTAAC GCCTACCAGC ACGACAAGGT GAACAACTGG 
ACCGGCCAGG TGGTGGAGAA CTGCCTGACG GTGCTCACCA AGGAGCAGAA GCCCTACAAG 
TACATTGTGA CCGCCATGAT CATGCAAAAG AACGGTGCTG GACTCCACAC CGCCAGCTCC 
TGCTACTGGA ACAACGACAC CGACGGATCG TGCACAGTAC GCTGGGAGAA CAAGACCATG 
TACTGCATCG TTTCGGTCTT CGGACTGGCC GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dlc90F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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