ATP8B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP8B
Entrez Gene ID	41469
Full Name	ATPase 8B
Synonyms	CG14741,DmCG14741,Dmel\CG14741,dATP8B
General protein information	Preferred Names ATPase 8B Names CG14741 gene product from transcript CG14741-RF ATP8B-PB ATP8B-PC ATP8B-PD ATP8B-PF ATP8B-PG ATP8B-PH ATP8B-PI CG14741-PB CG14741-PC CG14741-PD CG14741-PF CG14741-PG CG14741-PH CG14741-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87A7-87A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260138.2	NP_001247067.1	ATPase 8B, isoform C
NM_001260139.2	NP_001247068.1	ATPase 8B, isoform D
NM_169450.4	NP_731669.2	ATPase 8B, isoform B
NM_001300366.1	NP_001287295.1	ATPase 8B, isoform G
NM_001300365.1	NP_001287294.1	ATPase 8B, isoform F
NM_001260140.2	NP_001247069.2	ATPase 8B, isoform H
NM_001300367.1	NP_001287296.1	ATPase 8B, isoform I

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP8B2	XP_005622844.1
Mus musculus (house mouse)	Atp8b2	NP_001074651.2
Pan troglodytes (chimpanzee)	ATP8B2	XP_003949623.1
Rattus norvegicus (Norway rat)	LOC685152	XP_001062555.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002644	XP_312283.5
Homo sapiens (human)	ATP8B2	NP_065185.1
	ATP8B2	XP_001114383.1
Gallus gallus (chicken)	ATP8B2	XP_001233350.3
Caenorhabditis elegans (roundworm)	tat-2	NP_001023252.2
Bos taurus (cattle)	ATP8B2	NP_001178150.2
Drosophila melanogaster (fruit fly)	CG14741	NP_001247068.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dATP8B, the Drosophila homolog of mammalian ATP8B, is important for lipid translocation in olfactory neurons, which in turn is important for proper subcellular trafficking of receptor subunits.
Title: Lipid flippase modulates olfactory receptor expression and odorant sensitivity in Drosophila.

The following ATP8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa22085	NM_001300365.1 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa04637	NM_169450.4 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa08332	NM_001260138.2 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa08333	NM_001260139.2 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22086	NM_001300367.1 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22087	NM_001300366.1 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22088	NM_001260140.2 Latest version!	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP8B ( NM_001300365.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287294.1 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa22085
Clone ID Related Accession (Same CDS sequence)	NM_001300365.1
Accession Version	NM_001300365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGGCCCTT 
CAAAGTGTTG ACTTTTCGGC CAATCCGCAT CACGAGAGTG ACTTCCGCTG GTACGATCGC 
ACGTTGCTGG ATGCCGTCCG GTCGGATGAA GAGCACTCCC ATGTGTTTTT CCGCCTCCTG 
GCCCTCTGCC ACACGGTCAT GGCCGAAACG GTTGATGGTA AGTTGGAGTA CCAGGCCCAA 
AGTCCCGACG AAGCTGCCCT CGTTTCGGCC GCTCGAAATT TTGGCTTTGT CTTTCGCACA 
CGAACACCGA ATAGTATTAC CATCGAGGTG ATGGGACAAA CGGAGGAGTA CGAACTCCTA 
AATATCCTCG ACTTTAACAA CGTCCGCAAG CGGATGTCTG TGATTCTCCG GCGCGGTGAC 
TCCATGGTTC TCTACTGCAA AGGAGCGGAC AATGTGATAT ATGATCGTCT GCATGGCGGA 
CAGGAGGACC TTAAGGCGCG CACCCAGGAC CACCTTAATA AATTTGCCGG CGAAGGACTA 
CGTACTTTAG CGCTGGCTGA ACGGCGTTTA ACAGAGCAGT ACTACAACGA CTGGAGGAGT 
CGGCAACAGG AGGCAGCCCT TTCGATGGAC TCGAGGGAGC AGAAGCTGAA TGCCATTTAC 
GAGGAGATCG AGAGCGAGAT GCAGCTGGTC GGGGTGACCG CAATTGAGGA CAAACTGCAG 
GACGGCGTGC CCAAGTCAAT TGCTAATTTG CAGAATGCAG GCATAAAGAT ATGGGTCCTA 
ACCGGCGACA AGCAGGAAAC TGCCATCAAT ATCGGCTACT CCTGCCAACT GCTTACGGAT 
GAACTGGCGG ATGTCTTTAT CGTGGACGGC AATTCGGTGG AGGAAGTGGA AAAGCAGCTA 
AGGCAGTTTA AGGAATCTAT TAAGATTTAT AACCGGTTTC GACCAGGCGG ATTTGATCCA 
TTTGATCGCT TAAACAGCGA CAGCAACATG GATCCGCTAA GCGTGACCAT GACACAAACA 
TCGGCTTTCA TGCAGGAGAC GAACCTCCCG CCCACGCCGC CTCCGCCGCC CGCTATTTCG 
GTGGTTACCT TTAGGTGGGA TGAGAAAAAT AAGGATAATA AGGGCGGACC GGACAGTGCT 
GAGTGCAATA ACTTGTTCGG TGATGAAAAG GGAAGCGAAG ATGGAGGCAC TGCCTCCATT 
GTGGTGGATG AAAATACAGG ATTCGCTCTG GTGGTAAATG GTCATTCCCT GGTGCACTGT 
CTTTCGCCGG AATTGGAGAA CAAATTCCTG GACATCGCCT CGCAGTGCAA GGCGGTCATC 
TGTTGTCGGG TAACGCCACT TCAGAAGGCG CTGGTCGTCG AGCTAATAAA GCGTGCCAAA 
AACGCAGTCA CTCTGGCCAT TGGCGATGGT GCCAACGATG TGTCCATGAT TAAAGCTGCT 
CACATAGGCG TTGGAATCTC AGGACAGGAG GGTCTTCAGG CTGTCCTCTC CAGCGACTAT 
TCCATTGCCC AGTTTCGATA CCTTGAGCGA TTGTTACTGG TACATGGTCG ATGGTCCTAC 
TACCGCATGT GCAAGTTCCT CAGATACTTT TTCTACAAGA ACTTTGCATT TACACTGTGC 
CATTGCTGGT ATTCGCTCTT CTGCGGCTTC AGCGCTCAGA CGGTTTTCGA CCCGATGTTC 
ATATCAGTGT ATAATCTATT TTACACATCG CTACCCGTTT TGGCTTTGGG CGTCTTCGAA 
CAGGATGTCT CGGACAAGAA CAGTCTAGAG TTTCCGCGCC TCTACACTCC GGGTCTAAAG 
AGCGAGTTGT TCAACATTCG AGAGTTCATC TACAGTGTGT TGCACGGCGC CTTCACCTCA 
CTGGTCCTGT TCCTGATTCC TTATGGCGTC TACAAGGATG GCGTTTCGGC AAACGGATTT 
GTTGTGAGCG ATCACATGAC CCTGGGCGCT GTTGTCGCCA CCATACTTAT AGTGGATAAT 
ACAGCGCAGA TATCGTTGTA CACCTCCTAT TGGACCGTTG TGAATCATGT GACCATTTGG 
GGTAGTCTAG TTTGGTACTT TGTGCTGGAC TACTTCTACA ACTATGTGAT TGGTGGCCCT 
TACGTTGGCT CCTTGACTCA AGCTATGAAG GACCTGACCT TTTGGGTTAC CATGTTGATC 
ACGGTGATGG CGTTGGTGGC TCCTGTGTTG GCCTACAAGT TCTATTTGCT AGATGTACAT 
CCGAGCCTGT CGGATAAGAT TCGGCAAAAA TCTCTGAAGA AGATCCACTC CAGAGCTTCA 
AGTGATGTCA GGCGAACGGC CTCATCACGT CGTGGACGTC GCTCCGTACG TTCGGGTTAT 
GCCTTTGCCC ATCAGGAGGG CTTTGGTCGT CTGATAACCT CCGGCAAAAT TATGCACAAG 
CTGCCGCAGG ACTTTGCTTT TCCTCTGGGC TTGGGCACCA AGAAGACACA GGTGCTACAC 
AACAACCTGA ACTCGGCCGA TGGACCAAAT TCTAAGGGCA ACAATGTGAC TGGCCATCAT 
ATGGTCAACA ACAACACAAA TATGCGACAT AATCAGAACC AAAACCACTC GTCGATGGCA 
GACATAACGG CCGATGGGCG GGGCAATGGG GGAGATGATG GCAGAGGAAG TGGAGGCTCA 
GATGATATGA GTCCTCGTGC TCCCTGCCAG GACCTGGATA CGATTAATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287294.1
CDS	649..4602
Translation	MGTKTQPQLAKENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_001300365.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300365.1

ORF Insert Sequence:

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGGCCCTT 
CAAAGTGTTG ACTTTTCGGC CAATCCGCAT CACGAGAGTG ACTTCCGCTG GTACGATCGC 
ACGTTGCTGG ATGCCGTCCG GTCGGATGAA GAGCACTCCC ATGTGTTTTT CCGCCTCCTG 
GCCCTCTGCC ACACGGTCAT GGCCGAAACG GTTGATGGTA AGTTGGAGTA CCAGGCCCAA 
AGTCCCGACG AAGCTGCCCT CGTTTCGGCC GCTCGAAATT TTGGCTTTGT CTTTCGCACA 
CGAACACCGA ATAGTATTAC CATCGAGGTG ATGGGACAAA CGGAGGAGTA CGAACTCCTA 
AATATCCTCG ACTTTAACAA CGTCCGCAAG CGGATGTCTG TGATTCTCCG GCGCGGTGAC 
TCCATGGTTC TCTACTGCAA AGGAGCGGAC AATGTGATAT ATGATCGTCT GCATGGCGGA 
CAGGAGGACC TTAAGGCGCG CACCCAGGAC CACCTTAATA AATTTGCCGG CGAAGGACTA 
CGTACTTTAG CGCTGGCTGA ACGGCGTTTA ACAGAGCAGT ACTACAACGA CTGGAGGAGT 
CGGCAACAGG AGGCAGCCCT TTCGATGGAC TCGAGGGAGC AGAAGCTGAA TGCCATTTAC 
GAGGAGATCG AGAGCGAGAT GCAGCTGGTC GGGGTGACCG CAATTGAGGA CAAACTGCAG 
GACGGCGTGC CCAAGTCAAT TGCTAATTTG CAGAATGCAG GCATAAAGAT ATGGGTCCTA 
ACCGGCGACA AGCAGGAAAC TGCCATCAAT ATCGGCTACT CCTGCCAACT GCTTACGGAT 
GAACTGGCGG ATGTCTTTAT CGTGGACGGC AATTCGGTGG AGGAAGTGGA AAAGCAGCTA 
AGGCAGTTTA AGGAATCTAT TAAGATTTAT AACCGGTTTC GACCAGGCGG ATTTGATCCA 
TTTGATCGCT TAAACAGCGA CAGCAACATG GATCCGCTAA GCGTGACCAT GACACAAACA 
TCGGCTTTCA TGCAGGAGAC GAACCTCCCG CCCACGCCGC CTCCGCCGCC CGCTATTTCG 
GTGGTTACCT TTAGGTGGGA TGAGAAAAAT AAGGATAATA AGGGCGGACC GGACAGTGCT 
GAGTGCAATA ACTTGTTCGG TGATGAAAAG GGAAGCGAAG ATGGAGGCAC TGCCTCCATT 
GTGGTGGATG AAAATACAGG ATTCGCTCTG GTGGTAAATG GTCATTCCCT GGTGCACTGT 
CTTTCGCCGG AATTGGAGAA CAAATTCCTG GACATCGCCT CGCAGTGCAA GGCGGTCATC 
TGTTGTCGGG TAACGCCACT TCAGAAGGCG CTGGTCGTCG AGCTAATAAA GCGTGCCAAA 
AACGCAGTCA CTCTGGCCAT TGGCGATGGT GCCAACGATG TGTCCATGAT TAAAGCTGCT 
CACATAGGCG TTGGAATCTC AGGACAGGAG GGTCTTCAGG CTGTCCTCTC CAGCGACTAT 
TCCATTGCCC AGTTTCGATA CCTTGAGCGA TTGTTACTGG TACATGGTCG ATGGTCCTAC 
TACCGCATGT GCAAGTTCCT CAGATACTTT TTCTACAAGA ACTTTGCATT TACACTGTGC 
CATTGCTGGT ATTCGCTCTT CTGCGGCTTC AGCGCTCAGA CGGTTTTCGA CCCGATGTTC 
ATATCAGTGT ATAATCTATT TTACACATCG CTACCCGTTT TGGCTTTGGG CGTCTTCGAA 
CAGGATGTCT CGGACAAGAA CAGTCTAGAG TTTCCGCGCC TCTACACTCC GGGTCTAAAG 
AGCGAGTTGT TCAACATTCG AGAGTTCATC TACAGTGTGT TGCACGGCGC CTTCACCTCA 
CTGGTCCTGT TCCTGATTCC TTATGGCGTC TACAAGGATG GCGTTTCGGC AAACGGATTT 
GTTGTGAGCG ATCACATGAC CCTGGGCGCT GTTGTCGCCA CCATACTTAT AGTGGATAAT 
ACAGCGCAGA TATCGTTGTA CACCTCCTAT TGGACCGTTG TGAATCATGT GACCATTTGG 
GGTAGTCTAG TTTGGTACTT TGTGCTGGAC TACTTCTACA ACTATGTGAT TGGTGGCCCT 
TACGTTGGCT CCTTGACTCA AGCTATGAAG GACCTGACCT TTTGGGTTAC CATGTTGATC 
ACGGTGATGG CGTTGGTGGC TCCTGTGTTG GCCTACAAGT TCTATTTGCT AGATGTACAT 
CCGAGCCTGT CGGATAAGAT TCGGCAAAAA TCTCTGAAGA AGATCCACTC CAGAGCTTCA 
AGTGATGTCA GGCGAACGGC CTCATCACGT CGTGGACGTC GCTCCGTACG TTCGGGTTAT 
GCCTTTGCCC ATCAGGAGGG CTTTGGTCGT CTGATAACCT CCGGCAAAAT TATGCACAAG 
CTGCCGCAGG ACTTTGCTTT TCCTCTGGGC TTGGGCACCA AGAAGACACA GGTGCTACAC 
AACAACCTGA ACTCGGCCGA TGGACCAAAT TCTAAGGGCA ACAATGTGAC TGGCCATCAT 
ATGGTCAACA ACAACACAAA TATGCGACAT AATCAGAACC AAAACCACTC GTCGATGGCA 
GACATAACGG CCGATGGGCG GGGCAATGGG GGAGATGATG GCAGAGGAAG TGGAGGCTCA 
GATGATATGA GTCCTCGTGC TCCCTGCCAG GACCTGGATA CGATTAATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8B ( NM_169450.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731669.2 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04637
Clone ID Related Accession (Same CDS sequence)	NM_169450.4
Accession Version	NM_169450.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169450.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCGGGG GCAGGAGATG CAGCGAGGCC GCCCGGCGGC TAAGGAGGCG TCCTGACACT 
CTGGAACTTT CAGTTCTGGG GGGCCAACCT ATCGAAACTG AACTCCAGGA GGACAATGAG 
CGGACCCCAA CAAAGGGAGC CGGAAATGTG GATGAGCCCG GAATAGAAGC TCCTGAAGCT 
TCGTGCTCTG CGCCCAGCAA AAGAACCCGA TGGTTTCAGT TCGCCAGGAG CTCCAAAAAT 
CGAAGGAAAC GATTGCACTG TAATGAAGAT GAGGATGTTA AGCAGCCGGA AAGAAGGAAT 
TCCGATTTGG AGGGCAGTCC GAGGGCTTGC CTCGCTCAAC CGGAGGGCAT GAATATAGGC 
AGCTCTACGC AAACCATTGC CACACCCCTA ATGATAGGAG ATAGCTTCTA CAGCTTGGCG 
CCGGGAGGAG GCAATCCACC ACCCAACAAA TCCGATGACC AGGAACTGTA TAAGCAGAGA 
GATCCTTCCG TGACCTCGGA GTCAAAGTCC GTGCCAAGTT TGAGACGTCT TTCCCAGATT 
AGAAGGCGTC GCAGCTCGTA CTATTTTTCG GAAAACGAAC GTAGAATTCG AGCCAACGAT 
AAAGAGTTCA ATGCCCAGTT CAAATATCAT AACAACTACA TCAAGACCTC CAAGTATTCG 
CTGTTCACGT TTCTGCCATT CAATCTGCTG GAGCAATTTC AGCGGCTGGC CAACTTCTAT 
TTCCTCTGCC TGCTGGTCCT CCAGCTGATA CCCGCGATAT CCTCACTCAC CCCAGTGACC 
ACAGCAATTC CCCTGATAGG AGTACTTACT CTGACGGCTG TTAAGGATGC GTATGATGAT 
ATTCAACGTC ATATTTCTGA CTCGCAGGTA AACAATCGCA AGTCGAAAAC GCTGCGCAAT 
GGCAAGTTGG TGGAGGCCAA GTGGTCGGAG GTACAGGTGG GCGATGTCAT TCGGCTGGAC 
AACAATCAGT TCGTGGCCGC CGACACCCTG TTGCTGTCCA CATCCGAGCC AAATGGTCTG 
TGTTTCATCG AGACAGCCGA ACTGGATGGG GAGACGAATC TCAAGGCAAA GCAATGCCTG 
ACCGAAACCA TCGAGCTGGG TGATCGCCAT GACTCGCTAT GGAACTTCAA TGGCGAGATC 
ATTTGCGAGC GGCCCAACAA CCTGCTGAAC AAATTCGATG GCACCTTGAT TTGGCGGGGC 
CAGAGATTCG CCCTGGACAA CGAGAAGATC CTGCTGAGGG GCTGCGTCCT CCGGAACACG 
CAGTGGTGCT ATGGCGTGGT GGTCTTCGCC GGAGTGGACA CCAAGTTGAT GCAGAACTCC 
GGAAAGACGC AGTTCAAGAG CACTGGCGTG GATCGCCTGC TCAACTTTAT TATCATTGGG 
ATCGTCCTCT TTCTGGTGTC GATTTGTGCC CTTTTTGCGA TTGGCTGTGC CATCTGGGAG 
GGCCTGATTG GCCAGCACTT CCAGCTGTAT CTGCCTTGGG AGCACATCAT ACCCAAAGAT 
TACATACCCA CGGGAGCAAC AGTCATTGGA TTGCTGGTCT TCTTCTCGTA TGCCATAGTC 
TTAAACACAG TTGTGCCAAT CTCACTCTAC GTTTCAGTAG AGGTTATACG CTTTGTACAG 
TCGTTCCTCA TCAACTGGGA TGAGGAGATG TACTATCCGA CCACCAATAC CTATGCCAAA 
GCCCGCACCA CCACGCTCAA CGAGGAGCTG GGCCAGATCC AGTACATATT CTCGGATAAA 
ACGGGCACCC TCACCCAGAA CATCATGACC TTCAACAAGT GCAGCATCAA TGGGCGCAGC 
TATGGCGATG TGATTGACCT GCGCACCGGC GAGTTGGTCG AGATTACGGA GCAGCAAACA 
ATTTTCCAAA ATAGCAACAC CAACAACAGA CCCAGCCCCT TAAGTGGAGC TATCGTAGCA 
CCACCTGCAG CACCACCCCC CATCATCCTA GTGCACAAGG CCGAGGTCCA TGCGAAGAAG 
ACTTCCATGG TGGTCACATC CAGCGGGGAG GCGCAAGTGC TGCCAGACAG ACCCAGATCG 
GATATCGAGC GCTCTGCTCC GCCGATGGAC GCCAGCGAGA AGCGGCCGGG ACTCAAGCAT 
GTGCGATACT CGGCTCCCAG TAGAAGTCAG GATGCAGACG CTGGTCGTTT GTCACCCAGG 
TTGGATGGAA GTGGAGGACT TAGTCCACCC GTAGGCAATG AGGAGCGACG AATTAGTGGC 
GGCTTCAAAA GGAGTGGAGC CGGATGTATG CAGCGCCAAT TGTCCCGCAC TAGCAGTTGC 
GACAAAGTAA AGATTTTGCA TGACAGCCAA CAGACAGAAA CACACAACAT GCACCATTCA 
AGTCACAATC GCAAGCGATT AGTCAAGATC CGGTTTAAAA AAGCGCCATC AACGGCCACG 
CTGGGTGTTG TCATCTCCCA GCTCGAGCAC CACCTCGACG AGGAGCCACA ATCCACATCC 
ACACTTAGCA CCACCAAGCA CCACCACCAT CGATCCAAGG CATTCGCCAC CAATTGGAGT 
TCGTCGCCTC ACAGAGTGCA CGCCCTTCAA AGTGTTGACT TTTCGGCCAA TCCGCATCAC 
GAGAGTGACT TCCGCTGGTA CGATCGCACG TTGCTGGATG CCGTCCGGTC GGATGAAGAG 
CACTCCCATG TGTTTTTCCG CCTCCTGGCC CTCTGCCACA CGGTCATGGC CGAAACGGTT 
GATGGTAAGT TGGAGTACCA GGCCCAAAGT CCCGACGAAG CTGCCCTCGT TTCGGCCGCT 
CGAAATTTTG GCTTTGTCTT TCGCACACGA ACACCGAATA GTATTACCAT CGAGGTGATG 
GGACAAACGG AGGAGTACGA ACTCCTAAAT ATCCTCGACT TTAACAACGT CCGCAAGCGG 
ATGTCTGTGA TTCTCCGGCG CGGTGACTCC ATGGTTCTCT ACTGCAAAGG AGCGGACAAT 
GTGATATATG ATCGTCTGCA TGGCGGACAG GAGGACCTTA AGGCGCGCAC CCAGGACCAC 
CTTAATAAAT TTGCCGGCGA AGGACTACGT ACTTTAGCGC TGGCTGAACG GCGTTTAACA 
GAGCAGTACT ACAACGACTG GAGGAGTCGG CAACAGGAGG CAGCCCTTTC GATGGACTCG 
AGGGAGCAGA AGCTGAATGC CATTTACGAG GAGATCGAGA GCGAGATGCA GCTGGTCGGG 
GTGACCGCAA TTGAGGACAA ACTGCAGGAC GGCGTGCCCA AGTCAATTGC TAATTTGCAG 
AATGCAGGCA TAAAGATATG GGTCCTAACC GGCGACAAGC AGGAAACTGC CATCAATATC 
GGCTACTCCT GCCAACTGCT TACGGATGAA CTGGCGGATG TCTTTATCGT GGACGGCAAT 
TCGGTGGAGG AAGTGGAAAA GCAGCTAAGG CAGTTTAAGG AATCTATTAA GATTTATAAC 
CGGTTTCGAC CAGGCGGATT TGATCCATTT GATCGCTTAA ACAGCGACAG CAACATGGAT 
CCGCTAAGCG TGACCATGAC ACAAACATCG GCTTTCATGC AGGAGACGAA CCTCCCGCCC 
ACGCCGCCTC CGCCGCCCGC TATTTCGGTG GTTACCTTTA GGTGGGATGA GAAAAATAAG 
GATAATAAGG GCGGACCGGA CAGTGCTGAG TGCAATAACT TGTTCGGTGA TGAAAAGGGA 
AGCGAAGATG GAGGCACTGC CTCCATTGTG GTGGATGAAA ATACAGGATT CGCTCTGGTG 
GTAAATGGTC ATTCCCTGGT GCACTGTCTT TCGCCGGAAT TGGAGAACAA ATTCCTGGAC 
ATCGCCTCGC AGTGCAAGGC GGTCATCTGT TGTCGGGTAA CGCCACTTCA GAAGGCGCTG 
GTCGTCGAGC TAATAAAGCG TGCCAAAAAC GCAGTCACTC TGGCCATTGG CGATGGTGCC 
AACGATGTGT CCATGATTAA AGCTGCTCAC ATAGGCGTTG GAATCTCAGG ACAGGAGGGT 
CTTCAGGCTG TCCTCTCCAG CGACTATTCC ATTGCCCAGT TTCGATACCT TGAGCGATTG 
TTACTGGTAC ATGGTCGATG GTCCTACTAC CGCATGTGCA AGTTCCTCAG ATACTTTTTC 
TACAAGAACT TTGCATTTAC ACTGTGCCAT TGCTGGTATT CGCTCTTCTG CGGCTTCAGC 
GCTCAGACGG TTTTCGACCC GATGTTCATA TCAGTGTATA ATCTATTTTA CACATCGCTA 
CCCGTTTTGG CTTTGGGCGT CTTCGAACAG GATGTCTCGG ACAAGAACAG TCTAGAGTTT 
CCGCGCCTCT ACACTCCGGG TCTAAAGAGC GAGTTGTTCA ACATTCGAGA GTTCATCTAC 
AGTGTGTTGC ACGGCGCCTT CACCTCACTG GTCCTGTTCC TGATTCCTTA TGGCGTCTAC 
AAGGATGGCG TTTCGGCAAA CGGATTTGTT GTGAGCGATC ACATGACCCT GGGCGCTGTT 
GTCGCCACCA TACTTATAGT GGATAATACA GCGCAGATAT CGTTGTACAC CTCCTATTGG 
ACCGTTGTGA ATCATGTGAC CATTTGGGGT AGTCTAGTTT GGTACTTTGT GCTGGACTAC 
TTCTACAACT ATGTGATTGG TGGCCCTTAC GTTGGCTCCT TGACTCAAGC TATGAAGGAC 
CTGACCTTTT GGGTTACCAT GTTGATCACG GTGATGGCGT TGGTGGCTCC TGTGTTGGCC 
TACAAGTTCT ATTTGCTAGA TGTACATCCG AGCCTGTCGG ATAAGATTCG GCAAAAATCT 
CTGAAGAAGA TCCACTCCAG AGCTTCAAGT GATGTCAGGC GAACGGCCTC ATCACGTCGT 
GGACGTCGCT CCGTACGTTC GGGTTATGCC TTTGCCCATC AGGAGGGCTT TGGTCGTCTG 
ATAACCTCCG GCAAAATTAT GCACAAGCTG CCGCAGGACT TTGCTTTTCC TCTGGGCTTG 
GGCACCAAGA AGACACAGGT GCTACACAAC AACCTGAACT CGGCCGATGG ACCAAATTCT 
AAGGGCAACA ATGTGACTGG CCATCATATG GTCAACAACA ACACAAATAT GCGACATAAT 
CAGAACCAAA ACCACTCGTC GATGGCAGAC ATAACGGCCG ATGGGCGGGG CAATGGGGGA 
GATGATGGCA GAGGAAGTGG AGGCTCAGAT GATATGAGTC CTCGTGCTCC CTGCCAGGAC 
CTGGATACGA TTAATCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731669.2
CDS	309..5489
Translation	MIGGRRCSEAARRLRRRPDTLELSVLGGQPIETELQEDNERTPTKGAGNVDEPGIEAPEASCSAPSKRTRWFQFARSSKNRRKRLHCNEDEDVKQPERRNSDLEGSPRACLAQPEGMNIGSSTQTIATPLMIGDSFYSLAPGGGNPPPNKSDDQELYKQRDPSVTSESKSVPSLRRLSQIRRRRSSYYFSENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKVKILHDSQQTETHNMHHSSHNRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTSTLSTTKHHHHRSKAFATNWSSSPHRVHALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_169450.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169450.4

ORF Insert Sequence:

ATGATCGGGG GCAGGAGATG CAGCGAGGCC GCCCGGCGGC TAAGGAGGCG TCCTGACACT 
CTGGAACTTT CAGTTCTGGG GGGCCAACCT ATCGAAACTG AACTCCAGGA GGACAATGAG 
CGGACCCCAA CAAAGGGAGC CGGAAATGTG GATGAGCCCG GAATAGAAGC TCCTGAAGCT 
TCGTGCTCTG CGCCCAGCAA AAGAACCCGA TGGTTTCAGT TCGCCAGGAG CTCCAAAAAT 
CGAAGGAAAC GATTGCACTG TAATGAAGAT GAGGATGTTA AGCAGCCGGA AAGAAGGAAT 
TCCGATTTGG AGGGCAGTCC GAGGGCTTGC CTCGCTCAAC CGGAGGGCAT GAATATAGGC 
AGCTCTACGC AAACCATTGC CACACCCCTA ATGATAGGAG ATAGCTTCTA CAGCTTGGCG 
CCGGGAGGAG GCAATCCACC ACCCAACAAA TCCGATGACC AGGAACTGTA TAAGCAGAGA 
GATCCTTCCG TGACCTCGGA GTCAAAGTCC GTGCCAAGTT TGAGACGTCT TTCCCAGATT 
AGAAGGCGTC GCAGCTCGTA CTATTTTTCG GAAAACGAAC GTAGAATTCG AGCCAACGAT 
AAAGAGTTCA ATGCCCAGTT CAAATATCAT AACAACTACA TCAAGACCTC CAAGTATTCG 
CTGTTCACGT TTCTGCCATT CAATCTGCTG GAGCAATTTC AGCGGCTGGC CAACTTCTAT 
TTCCTCTGCC TGCTGGTCCT CCAGCTGATA CCCGCGATAT CCTCACTCAC CCCAGTGACC 
ACAGCAATTC CCCTGATAGG AGTACTTACT CTGACGGCTG TTAAGGATGC GTATGATGAT 
ATTCAACGTC ATATTTCTGA CTCGCAGGTA AACAATCGCA AGTCGAAAAC GCTGCGCAAT 
GGCAAGTTGG TGGAGGCCAA GTGGTCGGAG GTACAGGTGG GCGATGTCAT TCGGCTGGAC 
AACAATCAGT TCGTGGCCGC CGACACCCTG TTGCTGTCCA CATCCGAGCC AAATGGTCTG 
TGTTTCATCG AGACAGCCGA ACTGGATGGG GAGACGAATC TCAAGGCAAA GCAATGCCTG 
ACCGAAACCA TCGAGCTGGG TGATCGCCAT GACTCGCTAT GGAACTTCAA TGGCGAGATC 
ATTTGCGAGC GGCCCAACAA CCTGCTGAAC AAATTCGATG GCACCTTGAT TTGGCGGGGC 
CAGAGATTCG CCCTGGACAA CGAGAAGATC CTGCTGAGGG GCTGCGTCCT CCGGAACACG 
CAGTGGTGCT ATGGCGTGGT GGTCTTCGCC GGAGTGGACA CCAAGTTGAT GCAGAACTCC 
GGAAAGACGC AGTTCAAGAG CACTGGCGTG GATCGCCTGC TCAACTTTAT TATCATTGGG 
ATCGTCCTCT TTCTGGTGTC GATTTGTGCC CTTTTTGCGA TTGGCTGTGC CATCTGGGAG 
GGCCTGATTG GCCAGCACTT CCAGCTGTAT CTGCCTTGGG AGCACATCAT ACCCAAAGAT 
TACATACCCA CGGGAGCAAC AGTCATTGGA TTGCTGGTCT TCTTCTCGTA TGCCATAGTC 
TTAAACACAG TTGTGCCAAT CTCACTCTAC GTTTCAGTAG AGGTTATACG CTTTGTACAG 
TCGTTCCTCA TCAACTGGGA TGAGGAGATG TACTATCCGA CCACCAATAC CTATGCCAAA 
GCCCGCACCA CCACGCTCAA CGAGGAGCTG GGCCAGATCC AGTACATATT CTCGGATAAA 
ACGGGCACCC TCACCCAGAA CATCATGACC TTCAACAAGT GCAGCATCAA TGGGCGCAGC 
TATGGCGATG TGATTGACCT GCGCACCGGC GAGTTGGTCG AGATTACGGA GCAGCAAACA 
ATTTTCCAAA ATAGCAACAC CAACAACAGA CCCAGCCCCT TAAGTGGAGC TATCGTAGCA 
CCACCTGCAG CACCACCCCC CATCATCCTA GTGCACAAGG CCGAGGTCCA TGCGAAGAAG 
ACTTCCATGG TGGTCACATC CAGCGGGGAG GCGCAAGTGC TGCCAGACAG ACCCAGATCG 
GATATCGAGC GCTCTGCTCC GCCGATGGAC GCCAGCGAGA AGCGGCCGGG ACTCAAGCAT 
GTGCGATACT CGGCTCCCAG TAGAAGTCAG GATGCAGACG CTGGTCGTTT GTCACCCAGG 
TTGGATGGAA GTGGAGGACT TAGTCCACCC GTAGGCAATG AGGAGCGACG AATTAGTGGC 
GGCTTCAAAA GGAGTGGAGC CGGATGTATG CAGCGCCAAT TGTCCCGCAC TAGCAGTTGC 
GACAAAGTAA AGATTTTGCA TGACAGCCAA CAGACAGAAA CACACAACAT GCACCATTCA 
AGTCACAATC GCAAGCGATT AGTCAAGATC CGGTTTAAAA AAGCGCCATC AACGGCCACG 
CTGGGTGTTG TCATCTCCCA GCTCGAGCAC CACCTCGACG AGGAGCCACA ATCCACATCC 
ACACTTAGCA CCACCAAGCA CCACCACCAT CGATCCAAGG CATTCGCCAC CAATTGGAGT 
TCGTCGCCTC ACAGAGTGCA CGCCCTTCAA AGTGTTGACT TTTCGGCCAA TCCGCATCAC 
GAGAGTGACT TCCGCTGGTA CGATCGCACG TTGCTGGATG CCGTCCGGTC GGATGAAGAG 
CACTCCCATG TGTTTTTCCG CCTCCTGGCC CTCTGCCACA CGGTCATGGC CGAAACGGTT 
GATGGTAAGT TGGAGTACCA GGCCCAAAGT CCCGACGAAG CTGCCCTCGT TTCGGCCGCT 
CGAAATTTTG GCTTTGTCTT TCGCACACGA ACACCGAATA GTATTACCAT CGAGGTGATG 
GGACAAACGG AGGAGTACGA ACTCCTAAAT ATCCTCGACT TTAACAACGT CCGCAAGCGG 
ATGTCTGTGA TTCTCCGGCG CGGTGACTCC ATGGTTCTCT ACTGCAAAGG AGCGGACAAT 
GTGATATATG ATCGTCTGCA TGGCGGACAG GAGGACCTTA AGGCGCGCAC CCAGGACCAC 
CTTAATAAAT TTGCCGGCGA AGGACTACGT ACTTTAGCGC TGGCTGAACG GCGTTTAACA 
GAGCAGTACT ACAACGACTG GAGGAGTCGG CAACAGGAGG CAGCCCTTTC GATGGACTCG 
AGGGAGCAGA AGCTGAATGC CATTTACGAG GAGATCGAGA GCGAGATGCA GCTGGTCGGG 
GTGACCGCAA TTGAGGACAA ACTGCAGGAC GGCGTGCCCA AGTCAATTGC TAATTTGCAG 
AATGCAGGCA TAAAGATATG GGTCCTAACC GGCGACAAGC AGGAAACTGC CATCAATATC 
GGCTACTCCT GCCAACTGCT TACGGATGAA CTGGCGGATG TCTTTATCGT GGACGGCAAT 
TCGGTGGAGG AAGTGGAAAA GCAGCTAAGG CAGTTTAAGG AATCTATTAA GATTTATAAC 
CGGTTTCGAC CAGGCGGATT TGATCCATTT GATCGCTTAA ACAGCGACAG CAACATGGAT 
CCGCTAAGCG TGACCATGAC ACAAACATCG GCTTTCATGC AGGAGACGAA CCTCCCGCCC 
ACGCCGCCTC CGCCGCCCGC TATTTCGGTG GTTACCTTTA GGTGGGATGA GAAAAATAAG 
GATAATAAGG GCGGACCGGA CAGTGCTGAG TGCAATAACT TGTTCGGTGA TGAAAAGGGA 
AGCGAAGATG GAGGCACTGC CTCCATTGTG GTGGATGAAA ATACAGGATT CGCTCTGGTG 
GTAAATGGTC ATTCCCTGGT GCACTGTCTT TCGCCGGAAT TGGAGAACAA ATTCCTGGAC 
ATCGCCTCGC AGTGCAAGGC GGTCATCTGT TGTCGGGTAA CGCCACTTCA GAAGGCGCTG 
GTCGTCGAGC TAATAAAGCG TGCCAAAAAC GCAGTCACTC TGGCCATTGG CGATGGTGCC 
AACGATGTGT CCATGATTAA AGCTGCTCAC ATAGGCGTTG GAATCTCAGG ACAGGAGGGT 
CTTCAGGCTG TCCTCTCCAG CGACTATTCC ATTGCCCAGT TTCGATACCT TGAGCGATTG 
TTACTGGTAC ATGGTCGATG GTCCTACTAC CGCATGTGCA AGTTCCTCAG ATACTTTTTC 
TACAAGAACT TTGCATTTAC ACTGTGCCAT TGCTGGTATT CGCTCTTCTG CGGCTTCAGC 
GCTCAGACGG TTTTCGACCC GATGTTCATA TCAGTGTATA ATCTATTTTA CACATCGCTA 
CCCGTTTTGG CTTTGGGCGT CTTCGAACAG GATGTCTCGG ACAAGAACAG TCTAGAGTTT 
CCGCGCCTCT ACACTCCGGG TCTAAAGAGC GAGTTGTTCA ACATTCGAGA GTTCATCTAC 
AGTGTGTTGC ACGGCGCCTT CACCTCACTG GTCCTGTTCC TGATTCCTTA TGGCGTCTAC 
AAGGATGGCG TTTCGGCAAA CGGATTTGTT GTGAGCGATC ACATGACCCT GGGCGCTGTT 
GTCGCCACCA TACTTATAGT GGATAATACA GCGCAGATAT CGTTGTACAC CTCCTATTGG 
ACCGTTGTGA ATCATGTGAC CATTTGGGGT AGTCTAGTTT GGTACTTTGT GCTGGACTAC 
TTCTACAACT ATGTGATTGG TGGCCCTTAC GTTGGCTCCT TGACTCAAGC TATGAAGGAC 
CTGACCTTTT GGGTTACCAT GTTGATCACG GTGATGGCGT TGGTGGCTCC TGTGTTGGCC 
TACAAGTTCT ATTTGCTAGA TGTACATCCG AGCCTGTCGG ATAAGATTCG GCAAAAATCT 
CTGAAGAAGA TCCACTCCAG AGCTTCAAGT GATGTCAGGC GAACGGCCTC ATCACGTCGT 
GGACGTCGCT CCGTACGTTC GGGTTATGCC TTTGCCCATC AGGAGGGCTT TGGTCGTCTG 
ATAACCTCCG GCAAAATTAT GCACAAGCTG CCGCAGGACT TTGCTTTTCC TCTGGGCTTG 
GGCACCAAGA AGACACAGGT GCTACACAAC AACCTGAACT CGGCCGATGG ACCAAATTCT 
AAGGGCAACA ATGTGACTGG CCATCATATG GTCAACAACA ACACAAATAT GCGACATAAT 
CAGAACCAAA ACCACTCGTC GATGGCAGAC ATAACGGCCG ATGGGCGGGG CAATGGGGGA 
GATGATGGCA GAGGAAGTGG AGGCTCAGAT GATATGAGTC CTCGTGCTCC CTGCCAGGAC 
CTGGATACGA TTAATCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8B ( NM_001260138.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247067.1 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant C, mRNA.

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CloneID	OFa08332
Clone ID Related Accession (Same CDS sequence)	NM_001260138.2
Accession Version	NM_001260138.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260138.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG TAAAGATTTT GCATGACAGC CAACAGACAG AAACACACAA CATGCACCAT 
TCAAGTCACA ATCGCAAGCG ATTAGTCAAG ATCCGGTTTA AAAAAGCGCC ATCAACGGCC 
ACGCTGGGTG TTGTCATCTC CCAGCTCGAG CACCACCTCG ACGAGGAGCC ACAATCCACA 
TCCACACTTA GCACCACCAA GCACCACCAC CATCGATCCA AGGCATTCGC CACCAATTGG 
AGTTCGTCGC CTCACAGAGT GCACGCCCTT CAAAGTGTTG ACTTTTCGGC CAATCCGCAT 
CACGAGAGTG ACTTCCGCTG GTACGATCGC ACGTTGCTGG ATGCCGTCCG GTCGGATGAA 
GAGCACTCCC ATGTGTTTTT CCGCCTCCTG GCCCTCTGCC ACACGGTCAT GGCCGAAACG 
GTTGATGGTA AGTTGGAGTA CCAGGCCCAA AGTCCCGACG AAGCTGCCCT CGTTTCGGCC 
GCTCGAAATT TTGGCTTTGT CTTTCGCACA CGAACACCGA ATAGTATTAC CATCGAGGTG 
ATGGGACAAA CGGAGGAGTA CGAACTCCTA AATATCCTCG ACTTTAACAA CGTCCGCAAG 
CGGATGTCTG TGATTCTCCG GCGCGGTGAC TCCATGGTTC TCTACTGCAA AGGAGCGGAC 
AATGTGATAT ATGATCGTCT GCATGGCGGA CAGGAGGACC TTAAGGCGCG CACCCAGGAC 
CACCTTAATA AATTTGCCGG CGAAGGACTA CGTACTTTAG CGCTGGCTGA ACGGCGTTTA 
ACAGAGCAGT ACTACAACGA CTGGAGGAGT CGGCAACAGG AGGCAGCCCT TTCGATGGAC 
TCGAGGGAGC AGAAGCTGAA TGCCATTTAC GAGGAGATCG AGAGCGAGAT GCAGCTGGTC 
GGGGTGACCG CAATTGAGGA CAAACTGCAG GACGGCGTGC CCAAGTCAAT TGCTAATTTG 
CAGAATGCAG GCATAAAGAT ATGGGTCCTA ACCGGCGACA AGCAGGAAAC TGCCATCAAT 
ATCGGCTACT CCTGCCAACT GCTTACGGAT GAACTGGCGG ATGTCTTTAT CGTGGACGGC 
AATTCGGTGG AGGAAGTGGA AAAGCAGCTA AGGCAGTTTA AGGAATCTAT TAAGATTTAT 
AACCGGTTTC GACCAGGCGG ATTTGATCCA TTTGATCGCT TAAACAGCGA CAGCAACATG 
GATCCGCTAA GCGTGACCAT GACACAAACA TCGGCTTTCA TGCAGGAGAC GAACCTCCCG 
CCCACGCCGC CTCCGCCGCC CGCTATTTCG GTGGTTACCT TTAGGTGGGA TGAGAAAAAT 
AAGGATAATA AGGGCGGACC GGACAGTGCT GAGTGCAATA ACTTGTTCGG TGATGAAAAG 
GGAAGCGAAG ATGGAGGCAC TGCCTCCATT GTGGTGGATG AAAATACAGG ATTCGCTCTG 
GTGGTAAATG GTCATTCCCT GGTGCACTGT CTTTCGCCGG AATTGGAGAA CAAATTCCTG 
GACATCGCCT CGCAGTGCAA GGCGGTCATC TGTTGTCGGG TAACGCCACT TCAGAAGGCG 
CTGGTCGTCG AGCTAATAAA GCGTGCCAAA AACGCAGTCA CTCTGGCCAT TGGCGATGGT 
GCCAACGATG TGTCCATGAT TAAAGCTGCT CACATAGGCG TTGGAATCTC AGGACAGGAG 
GGTCTTCAGG CTGTCCTCTC CAGCGACTAT TCCATTGCCC AGTTTCGATA CCTTGAGCGA 
TTGTTACTGG TACATGGTCG ATGGTCCTAC TACCGCATGT GCAAGTTCCT CAGATACTTT 
TTCTACAAGA ACTTTGCATT TACACTGTGC CATTGCTGGT ATTCGCTCTT CTGCGGCTTC 
AGCGCTCAGA CGGTTTTCGA CCCGATGTTC ATATCAGTGT ATAATCTATT TTACACATCG 
CTACCCGTTT TGGCTTTGGG CGTCTTCGAA CAGGATGTCT CGGACAAGAA CAGTCTAGAG 
TTTCCGCGCC TCTACACTCC GGGTCTAAAG AGCGAGTTGT TCAACATTCG AGAGTTCATC 
TACAGTGTGT TGCACGGCGC CTTCACCTCA CTGGTCCTGT TCCTGATTCC TTATGGCGTC 
TACAAGGATG GCGTTTCGGC AAACGGATTT GTTGTGAGCG ATCACATGAC CCTGGGCGCT 
GTTGTCGCCA CCATACTTAT AGTGGATAAT ACAGCGCAGA TATCGTTGTA CACCTCCTAT 
TGGACCGTTG TGAATCATGT GACCATTTGG GGTAGTCTAG TTTGGTACTT TGTGCTGGAC 
TACTTCTACA ACTATGTGAT TGGTGGCCCT TACGTTGGCT CCTTGACTCA AGCTATGAAG 
GACCTGACCT TTTGGGTTAC CATGTTGATC ACGGTGATGG CGTTGGTGGC TCCTGTGTTG 
GCCTACAAGT TCTATTTGCT AGATGTACAT CCGAGCCTGT CGGATAAGAT TCGGCAAAAA 
TCTCTGAAGA AGATCCACTC CAGAGCTTCA AGTGATGTCA GGCGAACGGC CTCATCACGT 
CGTGGACGTC GCTCCGTACG TTCGGGTTAT GCCTTTGCCC ATCAGGAGGG CTTTGGTCGT 
CTGATAACCT CCGGCAAAAT TATGCACAAG CTGCCGCAGG ACTTTGCTTT TCCTCTGGGC 
TTGGGCACCA AGAAGACACA GGTGCTACAC AACAACCTGA ACTCGGCCGA TGGACCAAAT 
TCTAAGGGCA ACAATGTGAC TGGCCATCAT ATGGTCAACA ACAACACAAA TATGCGACAT 
AATCAGAACC AAAACCACTC GTCGATGGCA GACATAACGG CCGATGGGCG GGGCAATGGG 
GGAGATGATG GCAGAGGAAG TGGAGGCTCA GATGATATGA GTCCTCGTGC TCCCTGCCAG 
GACCTGGATA CGATTAATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247067.1
CDS	649..5292
Translation	MGTKTQPQLAKENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKVKILHDSQQTETHNMHHSSHNRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTSTLSTTKHHHHRSKAFATNWSSSPHRVHALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_001260138.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260138.2

ORF Insert Sequence:

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG TAAAGATTTT GCATGACAGC CAACAGACAG AAACACACAA CATGCACCAT 
TCAAGTCACA ATCGCAAGCG ATTAGTCAAG ATCCGGTTTA AAAAAGCGCC ATCAACGGCC 
ACGCTGGGTG TTGTCATCTC CCAGCTCGAG CACCACCTCG ACGAGGAGCC ACAATCCACA 
TCCACACTTA GCACCACCAA GCACCACCAC CATCGATCCA AGGCATTCGC CACCAATTGG 
AGTTCGTCGC CTCACAGAGT GCACGCCCTT CAAAGTGTTG ACTTTTCGGC CAATCCGCAT 
CACGAGAGTG ACTTCCGCTG GTACGATCGC ACGTTGCTGG ATGCCGTCCG GTCGGATGAA 
GAGCACTCCC ATGTGTTTTT CCGCCTCCTG GCCCTCTGCC ACACGGTCAT GGCCGAAACG 
GTTGATGGTA AGTTGGAGTA CCAGGCCCAA AGTCCCGACG AAGCTGCCCT CGTTTCGGCC 
GCTCGAAATT TTGGCTTTGT CTTTCGCACA CGAACACCGA ATAGTATTAC CATCGAGGTG 
ATGGGACAAA CGGAGGAGTA CGAACTCCTA AATATCCTCG ACTTTAACAA CGTCCGCAAG 
CGGATGTCTG TGATTCTCCG GCGCGGTGAC TCCATGGTTC TCTACTGCAA AGGAGCGGAC 
AATGTGATAT ATGATCGTCT GCATGGCGGA CAGGAGGACC TTAAGGCGCG CACCCAGGAC 
CACCTTAATA AATTTGCCGG CGAAGGACTA CGTACTTTAG CGCTGGCTGA ACGGCGTTTA 
ACAGAGCAGT ACTACAACGA CTGGAGGAGT CGGCAACAGG AGGCAGCCCT TTCGATGGAC 
TCGAGGGAGC AGAAGCTGAA TGCCATTTAC GAGGAGATCG AGAGCGAGAT GCAGCTGGTC 
GGGGTGACCG CAATTGAGGA CAAACTGCAG GACGGCGTGC CCAAGTCAAT TGCTAATTTG 
CAGAATGCAG GCATAAAGAT ATGGGTCCTA ACCGGCGACA AGCAGGAAAC TGCCATCAAT 
ATCGGCTACT CCTGCCAACT GCTTACGGAT GAACTGGCGG ATGTCTTTAT CGTGGACGGC 
AATTCGGTGG AGGAAGTGGA AAAGCAGCTA AGGCAGTTTA AGGAATCTAT TAAGATTTAT 
AACCGGTTTC GACCAGGCGG ATTTGATCCA TTTGATCGCT TAAACAGCGA CAGCAACATG 
GATCCGCTAA GCGTGACCAT GACACAAACA TCGGCTTTCA TGCAGGAGAC GAACCTCCCG 
CCCACGCCGC CTCCGCCGCC CGCTATTTCG GTGGTTACCT TTAGGTGGGA TGAGAAAAAT 
AAGGATAATA AGGGCGGACC GGACAGTGCT GAGTGCAATA ACTTGTTCGG TGATGAAAAG 
GGAAGCGAAG ATGGAGGCAC TGCCTCCATT GTGGTGGATG AAAATACAGG ATTCGCTCTG 
GTGGTAAATG GTCATTCCCT GGTGCACTGT CTTTCGCCGG AATTGGAGAA CAAATTCCTG 
GACATCGCCT CGCAGTGCAA GGCGGTCATC TGTTGTCGGG TAACGCCACT TCAGAAGGCG 
CTGGTCGTCG AGCTAATAAA GCGTGCCAAA AACGCAGTCA CTCTGGCCAT TGGCGATGGT 
GCCAACGATG TGTCCATGAT TAAAGCTGCT CACATAGGCG TTGGAATCTC AGGACAGGAG 
GGTCTTCAGG CTGTCCTCTC CAGCGACTAT TCCATTGCCC AGTTTCGATA CCTTGAGCGA 
TTGTTACTGG TACATGGTCG ATGGTCCTAC TACCGCATGT GCAAGTTCCT CAGATACTTT 
TTCTACAAGA ACTTTGCATT TACACTGTGC CATTGCTGGT ATTCGCTCTT CTGCGGCTTC 
AGCGCTCAGA CGGTTTTCGA CCCGATGTTC ATATCAGTGT ATAATCTATT TTACACATCG 
CTACCCGTTT TGGCTTTGGG CGTCTTCGAA CAGGATGTCT CGGACAAGAA CAGTCTAGAG 
TTTCCGCGCC TCTACACTCC GGGTCTAAAG AGCGAGTTGT TCAACATTCG AGAGTTCATC 
TACAGTGTGT TGCACGGCGC CTTCACCTCA CTGGTCCTGT TCCTGATTCC TTATGGCGTC 
TACAAGGATG GCGTTTCGGC AAACGGATTT GTTGTGAGCG ATCACATGAC CCTGGGCGCT 
GTTGTCGCCA CCATACTTAT AGTGGATAAT ACAGCGCAGA TATCGTTGTA CACCTCCTAT 
TGGACCGTTG TGAATCATGT GACCATTTGG GGTAGTCTAG TTTGGTACTT TGTGCTGGAC 
TACTTCTACA ACTATGTGAT TGGTGGCCCT TACGTTGGCT CCTTGACTCA AGCTATGAAG 
GACCTGACCT TTTGGGTTAC CATGTTGATC ACGGTGATGG CGTTGGTGGC TCCTGTGTTG 
GCCTACAAGT TCTATTTGCT AGATGTACAT CCGAGCCTGT CGGATAAGAT TCGGCAAAAA 
TCTCTGAAGA AGATCCACTC CAGAGCTTCA AGTGATGTCA GGCGAACGGC CTCATCACGT 
CGTGGACGTC GCTCCGTACG TTCGGGTTAT GCCTTTGCCC ATCAGGAGGG CTTTGGTCGT 
CTGATAACCT CCGGCAAAAT TATGCACAAG CTGCCGCAGG ACTTTGCTTT TCCTCTGGGC 
TTGGGCACCA AGAAGACACA GGTGCTACAC AACAACCTGA ACTCGGCCGA TGGACCAAAT 
TCTAAGGGCA ACAATGTGAC TGGCCATCAT ATGGTCAACA ACAACACAAA TATGCGACAT 
AATCAGAACC AAAACCACTC GTCGATGGCA GACATAACGG CCGATGGGCG GGGCAATGGG 
GGAGATGATG GCAGAGGAAG TGGAGGCTCA GATGATATGA GTCCTCGTGC TCCCTGCCAG 
GACCTGGATA CGATTAATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8B ( NM_001260139.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247068.1 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant D, mRNA.

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CloneID	OFa08333
Clone ID Related Accession (Same CDS sequence)	NM_001260139.2
Accession Version	NM_001260139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260139.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCGGGG GCAGGAGATG CAGCGAGGCC GCCCGGCGGC TAAGGAGGCG TCCTGACACT 
CTGGAACTTT CAGTTCTGGG GGGCCAACCT ATCGAAACTG AACTCCAGGA GGACAATGAG 
CGGACCCCAA CAAAGGGAGC CGGAAATGTG GATGAGCCCG GAATAGAAGC TCCTGAAGCT 
TCGTGCTCTG CGCCCAGCAA AAGAACCCGA TGGTTTCAGT TCGCCAGGAG CTCCAAAAAT 
CGAAGGAAAC GATTGCACTG TAATGAAGAT GAGGATGTTA AGCAGCCGGA AAGAAGGAAT 
TCCGATTTGG AGGGCAGTCC GAGGGCTTGC CTCGCTCAAC CGGAGGGCAT GAATATAGGC 
AGCTCTACGC AAACCATTGC CACACCCCTA ATGATAGGAG ATAGCTTCTA CAGCTTGGCG 
CCGGGAGGAG GCAATCCACC ACCCAACAAA TCCGATGACC AGGAACTGTA TAAGCAGAGA 
GATCCTTCCG TGACCTCGGA GTCAAAGTCC GTGCCAAGTT TGAGACGTCT TTCCCAGATT 
AGAAGGCGTC GCAGCTCGTA CTATTTTTCG GAAAACGAAC GTAGAATTCG AGCCAACGAT 
AAAGAGTTCA ATGCCCAGTT CAAATATCAT AACAACTACA TCAAGACCTC CAAGTATTCG 
CTGTTCACGT TTCTGCCATT CAATCTGCTG GAGCAATTTC AGCGGCTGGC CAACTTCTAT 
TTCCTCTGCC TGCTGGTCCT CCAGCTGATA CCCGCGATAT CCTCACTCAC CCCAGTGACC 
ACAGCAATTC CCCTGATAGG AGTACTTACT CTGACGGCTG TTAAGGATGC GTATGATGAT 
ATTCAACGTC ATATTTCTGA CTCGCAGGTA AACAATCGCA AGTCGAAAAC GCTGCGCAAT 
GGCAAGTTGG TGGAGGCCAA GTGGTCGGAG GTACAGGTGG GCGATGTCAT TCGGCTGGAC 
AACAATCAGT TCGTGGCCGC CGACACCCTG TTGCTGTCCA CATCCGAGCC AAATGGTCTG 
TGTTTCATCG AGACAGCCGA ACTGGATGGG GAGACGAATC TCAAGGCAAA GCAATGCCTG 
ACCGAAACCA TCGAGCTGGG TGATCGCCAT GACTCGCTAT GGAACTTCAA TGGCGAGATC 
ATTTGCGAGC GGCCCAACAA CCTGCTGAAC AAATTCGATG GCACCTTGAT TTGGCGGGGC 
CAGAGATTCG CCCTGGACAA CGAGAAGATC CTGCTGAGGG GCTGCGTCCT CCGGAACACG 
CAGTGGTGCT ATGGCGTGGT GGTCTTCGCC GGAGTGGACA CCAAGTTGAT GCAGAACTCC 
GGAAAGACGC AGTTCAAGAG CACTGGCGTG GATCGCCTGC TCAACTTTAT TATCATTGGG 
ATCGTCCTCT TTCTGGTGTC GATTTGTGCC CTTTTTGCGA TTGGCTGTGC CATCTGGGAG 
GGCCTGATTG GCCAGCACTT CCAGCTGTAT CTGCCTTGGG AGCACATCAT ACCCAAAGAT 
TACATACCCA CGGGAGCAAC AGTCATTGGA TTGCTGGTCT TCTTCTCGTA TGCCATAGTC 
TTAAACACAG TTGTGCCAAT CTCACTCTAC GTTTCAGTAG AGGTTATACG CTTTGTACAG 
TCGTTCCTCA TCAACTGGGA TGAGGAGATG TACTATCCGA CCACCAATAC CTATGCCAAA 
GCCCGCACCA CCACGCTCAA CGAGGAGCTG GGCCAGATCC AGTACATATT CTCGGATAAA 
ACGGGCACCC TCACCCAGAA CATCATGACC TTCAACAAGT GCAGCATCAA TGGGCGCAGC 
TATGGCGATG TGATTGACCT GCGCACCGGC GAGTTGGTCG AGATTACGGA GGCCCTTCAA 
AGTGTTGACT TTTCGGCCAA TCCGCATCAC GAGAGTGACT TCCGCTGGTA CGATCGCACG 
TTGCTGGATG CCGTCCGGTC GGATGAAGAG CACTCCCATG TGTTTTTCCG CCTCCTGGCC 
CTCTGCCACA CGGTCATGGC CGAAACGGTT GATGGTAAGT TGGAGTACCA GGCCCAAAGT 
CCCGACGAAG CTGCCCTCGT TTCGGCCGCT CGAAATTTTG GCTTTGTCTT TCGCACACGA 
ACACCGAATA GTATTACCAT CGAGGTGATG GGACAAACGG AGGAGTACGA ACTCCTAAAT 
ATCCTCGACT TTAACAACGT CCGCAAGCGG ATGTCTGTGA TTCTCCGGCG CGGTGACTCC 
ATGGTTCTCT ACTGCAAAGG AGCGGACAAT GTGATATATG ATCGTCTGCA TGGCGGACAG 
GAGGACCTTA AGGCGCGCAC CCAGGACCAC CTTAATAAAT TTGCCGGCGA AGGACTACGT 
ACTTTAGCGC TGGCTGAACG GCGTTTAACA GAGCAGTACT ACAACGACTG GAGGAGTCGG 
CAACAGGAGG CAGCCCTTTC GATGGACTCG AGGGAGCAGA AGCTGAATGC CATTTACGAG 
GAGATCGAGA GCGAGATGCA GCTGGTCGGG GTGACCGCAA TTGAGGACAA ACTGCAGGAC 
GGCGTGCCCA AGTCAATTGC TAATTTGCAG AATGCAGGCA TAAAGATATG GGTCCTAACC 
GGCGACAAGC AGGAAACTGC CATCAATATC GGCTACTCCT GCCAACTGCT TACGGATGAA 
CTGGCGGATG TCTTTATCGT GGACGGCAAT TCGGTGGAGG AAGTGGAAAA GCAGCTAAGG 
CAGTTTAAGG AATCTATTAA GATTTATAAC CGGTTTCGAC CAGGCGGATT TGATCCATTT 
GATCGCTTAA ACAGCGACAG CAACATGGAT CCGCTAAGCG TGACCATGAC ACAAACATCG 
GCTTTCATGC AGGAGACGAA CCTCCCGCCC ACGCCGCCTC CGCCGCCCGC TATTTCGGTG 
GTTACCTTTA GGTGGGATGA GAAAAATAAG GATAATAAGG GCGGACCGGA CAGTGCTGAG 
TGCAATAACT TGTTCGGTGA TGAAAAGGGA AGCGAAGATG GAGGCACTGC CTCCATTGTG 
GTGGATGAAA ATACAGGATT CGCTCTGGTG GTAAATGGTC ATTCCCTGGT GCACTGTCTT 
TCGCCGGAAT TGGAGAACAA ATTCCTGGAC ATCGCCTCGC AGTGCAAGGC GGTCATCTGT 
TGTCGGGTAA CGCCACTTCA GAAGGCGCTG GTCGTCGAGC TAATAAAGCG TGCCAAAAAC 
GCAGTCACTC TGGCCATTGG CGATGGTGCC AACGATGTGT CCATGATTAA AGCTGCTCAC 
ATAGGCGTTG GAATCTCAGG ACAGGAGGGT CTTCAGGCTG TCCTCTCCAG CGACTATTCC 
ATTGCCCAGT TTCGATACCT TGAGCGATTG TTACTGGTAC ATGGTCGATG GTCCTACTAC 
CGCATGTGCA AGTTCCTCAG ATACTTTTTC TACAAGAACT TTGCATTTAC ACTGTGCCAT 
TGCTGGTATT CGCTCTTCTG CGGCTTCAGC GCTCAGACGG TTTTCGACCC GATGTTCATA 
TCAGTGTATA ATCTATTTTA CACATCGCTA CCCGTTTTGG CTTTGGGCGT CTTCGAACAG 
GATGTCTCGG ACAAGAACAG TCTAGAGTTT CCGCGCCTCT ACACTCCGGG TCTAAAGAGC 
GAGTTGTTCA ACATTCGAGA GTTCATCTAC AGTGTGTTGC ACGGCGCCTT CACCTCACTG 
GTCCTGTTCC TGATTCCTTA TGGCGTCTAC AAGGATGGCG TTTCGGCAAA CGGATTTGTT 
GTGAGCGATC ACATGACCCT GGGCGCTGTT GTCGCCACCA TACTTATAGT GGATAATACA 
GCGCAGATAT CGTTGTACAC CTCCTATTGG ACCGTTGTGA ATCATGTGAC CATTTGGGGT 
AGTCTAGTTT GGTACTTTGT GCTGGACTAC TTCTACAACT ATGTGATTGG TGGCCCTTAC 
GTTGGCTCCT TGACTCAAGC TATGAAGGAC CTGACCTTTT GGGTTACCAT GTTGATCACG 
GTGATGGCGT TGGTGGCTCC TGTGTTGGCC TACAAGTTCT ATTTGCTAGA TGTACATCCG 
AGCCTGTCGG ATAAGATTCG GCAAAAATCT CTGAAGAAGA TCCACTCCAG AGCTTCAAGT 
GATGTCAGGC GAACGGCCTC ATCACGTCGT GGACGTCGCT CCGTACGTTC GGGTTATGCC 
TTTGCCCATC AGGAGGGCTT TGGTCGTCTG ATAACCTCCG GCAAAATTAT GCACAAGCTG 
CCGCAGGACT TTGCTTTTCC TCTGGGCTTG GGCACCAAGA AGACACAGGT GCTACACAAC 
AACCTGAACT CGGCCGATGG ACCAAATTCT AAGGGCAACA ATGTGACTGG CCATCATATG 
GTCAACAACA ACACAAATAT GCGACATAAT CAGAACCAAA ACCACTCGTC GATGGCAGAC 
ATAACGGCCG ATGGGCGGGG CAATGGGGGA GATGATGGCA GAGGAAGTGG AGGCTCAGAT 
GATATGAGTC CTCGTGCTCC CTGCCAGGAC CTGGATACGA TTAATCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247068.1
CDS	309..4799
Translation	MIGGRRCSEAARRLRRRPDTLELSVLGGQPIETELQEDNERTPTKGAGNVDEPGIEAPEASCSAPSKRTRWFQFARSSKNRRKRLHCNEDEDVKQPERRNSDLEGSPRACLAQPEGMNIGSSTQTIATPLMIGDSFYSLAPGGGNPPPNKSDDQELYKQRDPSVTSESKSVPSLRRLSQIRRRRSSYYFSENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_001260139.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260139.2

ORF Insert Sequence:

ATGATCGGGG GCAGGAGATG CAGCGAGGCC GCCCGGCGGC TAAGGAGGCG TCCTGACACT 
CTGGAACTTT CAGTTCTGGG GGGCCAACCT ATCGAAACTG AACTCCAGGA GGACAATGAG 
CGGACCCCAA CAAAGGGAGC CGGAAATGTG GATGAGCCCG GAATAGAAGC TCCTGAAGCT 
TCGTGCTCTG CGCCCAGCAA AAGAACCCGA TGGTTTCAGT TCGCCAGGAG CTCCAAAAAT 
CGAAGGAAAC GATTGCACTG TAATGAAGAT GAGGATGTTA AGCAGCCGGA AAGAAGGAAT 
TCCGATTTGG AGGGCAGTCC GAGGGCTTGC CTCGCTCAAC CGGAGGGCAT GAATATAGGC 
AGCTCTACGC AAACCATTGC CACACCCCTA ATGATAGGAG ATAGCTTCTA CAGCTTGGCG 
CCGGGAGGAG GCAATCCACC ACCCAACAAA TCCGATGACC AGGAACTGTA TAAGCAGAGA 
GATCCTTCCG TGACCTCGGA GTCAAAGTCC GTGCCAAGTT TGAGACGTCT TTCCCAGATT 
AGAAGGCGTC GCAGCTCGTA CTATTTTTCG GAAAACGAAC GTAGAATTCG AGCCAACGAT 
AAAGAGTTCA ATGCCCAGTT CAAATATCAT AACAACTACA TCAAGACCTC CAAGTATTCG 
CTGTTCACGT TTCTGCCATT CAATCTGCTG GAGCAATTTC AGCGGCTGGC CAACTTCTAT 
TTCCTCTGCC TGCTGGTCCT CCAGCTGATA CCCGCGATAT CCTCACTCAC CCCAGTGACC 
ACAGCAATTC CCCTGATAGG AGTACTTACT CTGACGGCTG TTAAGGATGC GTATGATGAT 
ATTCAACGTC ATATTTCTGA CTCGCAGGTA AACAATCGCA AGTCGAAAAC GCTGCGCAAT 
GGCAAGTTGG TGGAGGCCAA GTGGTCGGAG GTACAGGTGG GCGATGTCAT TCGGCTGGAC 
AACAATCAGT TCGTGGCCGC CGACACCCTG TTGCTGTCCA CATCCGAGCC AAATGGTCTG 
TGTTTCATCG AGACAGCCGA ACTGGATGGG GAGACGAATC TCAAGGCAAA GCAATGCCTG 
ACCGAAACCA TCGAGCTGGG TGATCGCCAT GACTCGCTAT GGAACTTCAA TGGCGAGATC 
ATTTGCGAGC GGCCCAACAA CCTGCTGAAC AAATTCGATG GCACCTTGAT TTGGCGGGGC 
CAGAGATTCG CCCTGGACAA CGAGAAGATC CTGCTGAGGG GCTGCGTCCT CCGGAACACG 
CAGTGGTGCT ATGGCGTGGT GGTCTTCGCC GGAGTGGACA CCAAGTTGAT GCAGAACTCC 
GGAAAGACGC AGTTCAAGAG CACTGGCGTG GATCGCCTGC TCAACTTTAT TATCATTGGG 
ATCGTCCTCT TTCTGGTGTC GATTTGTGCC CTTTTTGCGA TTGGCTGTGC CATCTGGGAG 
GGCCTGATTG GCCAGCACTT CCAGCTGTAT CTGCCTTGGG AGCACATCAT ACCCAAAGAT 
TACATACCCA CGGGAGCAAC AGTCATTGGA TTGCTGGTCT TCTTCTCGTA TGCCATAGTC 
TTAAACACAG TTGTGCCAAT CTCACTCTAC GTTTCAGTAG AGGTTATACG CTTTGTACAG 
TCGTTCCTCA TCAACTGGGA TGAGGAGATG TACTATCCGA CCACCAATAC CTATGCCAAA 
GCCCGCACCA CCACGCTCAA CGAGGAGCTG GGCCAGATCC AGTACATATT CTCGGATAAA 
ACGGGCACCC TCACCCAGAA CATCATGACC TTCAACAAGT GCAGCATCAA TGGGCGCAGC 
TATGGCGATG TGATTGACCT GCGCACCGGC GAGTTGGTCG AGATTACGGA GGCCCTTCAA 
AGTGTTGACT TTTCGGCCAA TCCGCATCAC GAGAGTGACT TCCGCTGGTA CGATCGCACG 
TTGCTGGATG CCGTCCGGTC GGATGAAGAG CACTCCCATG TGTTTTTCCG CCTCCTGGCC 
CTCTGCCACA CGGTCATGGC CGAAACGGTT GATGGTAAGT TGGAGTACCA GGCCCAAAGT 
CCCGACGAAG CTGCCCTCGT TTCGGCCGCT CGAAATTTTG GCTTTGTCTT TCGCACACGA 
ACACCGAATA GTATTACCAT CGAGGTGATG GGACAAACGG AGGAGTACGA ACTCCTAAAT 
ATCCTCGACT TTAACAACGT CCGCAAGCGG ATGTCTGTGA TTCTCCGGCG CGGTGACTCC 
ATGGTTCTCT ACTGCAAAGG AGCGGACAAT GTGATATATG ATCGTCTGCA TGGCGGACAG 
GAGGACCTTA AGGCGCGCAC CCAGGACCAC CTTAATAAAT TTGCCGGCGA AGGACTACGT 
ACTTTAGCGC TGGCTGAACG GCGTTTAACA GAGCAGTACT ACAACGACTG GAGGAGTCGG 
CAACAGGAGG CAGCCCTTTC GATGGACTCG AGGGAGCAGA AGCTGAATGC CATTTACGAG 
GAGATCGAGA GCGAGATGCA GCTGGTCGGG GTGACCGCAA TTGAGGACAA ACTGCAGGAC 
GGCGTGCCCA AGTCAATTGC TAATTTGCAG AATGCAGGCA TAAAGATATG GGTCCTAACC 
GGCGACAAGC AGGAAACTGC CATCAATATC GGCTACTCCT GCCAACTGCT TACGGATGAA 
CTGGCGGATG TCTTTATCGT GGACGGCAAT TCGGTGGAGG AAGTGGAAAA GCAGCTAAGG 
CAGTTTAAGG AATCTATTAA GATTTATAAC CGGTTTCGAC CAGGCGGATT TGATCCATTT 
GATCGCTTAA ACAGCGACAG CAACATGGAT CCGCTAAGCG TGACCATGAC ACAAACATCG 
GCTTTCATGC AGGAGACGAA CCTCCCGCCC ACGCCGCCTC CGCCGCCCGC TATTTCGGTG 
GTTACCTTTA GGTGGGATGA GAAAAATAAG GATAATAAGG GCGGACCGGA CAGTGCTGAG 
TGCAATAACT TGTTCGGTGA TGAAAAGGGA AGCGAAGATG GAGGCACTGC CTCCATTGTG 
GTGGATGAAA ATACAGGATT CGCTCTGGTG GTAAATGGTC ATTCCCTGGT GCACTGTCTT 
TCGCCGGAAT TGGAGAACAA ATTCCTGGAC ATCGCCTCGC AGTGCAAGGC GGTCATCTGT 
TGTCGGGTAA CGCCACTTCA GAAGGCGCTG GTCGTCGAGC TAATAAAGCG TGCCAAAAAC 
GCAGTCACTC TGGCCATTGG CGATGGTGCC AACGATGTGT CCATGATTAA AGCTGCTCAC 
ATAGGCGTTG GAATCTCAGG ACAGGAGGGT CTTCAGGCTG TCCTCTCCAG CGACTATTCC 
ATTGCCCAGT TTCGATACCT TGAGCGATTG TTACTGGTAC ATGGTCGATG GTCCTACTAC 
CGCATGTGCA AGTTCCTCAG ATACTTTTTC TACAAGAACT TTGCATTTAC ACTGTGCCAT 
TGCTGGTATT CGCTCTTCTG CGGCTTCAGC GCTCAGACGG TTTTCGACCC GATGTTCATA 
TCAGTGTATA ATCTATTTTA CACATCGCTA CCCGTTTTGG CTTTGGGCGT CTTCGAACAG 
GATGTCTCGG ACAAGAACAG TCTAGAGTTT CCGCGCCTCT ACACTCCGGG TCTAAAGAGC 
GAGTTGTTCA ACATTCGAGA GTTCATCTAC AGTGTGTTGC ACGGCGCCTT CACCTCACTG 
GTCCTGTTCC TGATTCCTTA TGGCGTCTAC AAGGATGGCG TTTCGGCAAA CGGATTTGTT 
GTGAGCGATC ACATGACCCT GGGCGCTGTT GTCGCCACCA TACTTATAGT GGATAATACA 
GCGCAGATAT CGTTGTACAC CTCCTATTGG ACCGTTGTGA ATCATGTGAC CATTTGGGGT 
AGTCTAGTTT GGTACTTTGT GCTGGACTAC TTCTACAACT ATGTGATTGG TGGCCCTTAC 
GTTGGCTCCT TGACTCAAGC TATGAAGGAC CTGACCTTTT GGGTTACCAT GTTGATCACG 
GTGATGGCGT TGGTGGCTCC TGTGTTGGCC TACAAGTTCT ATTTGCTAGA TGTACATCCG 
AGCCTGTCGG ATAAGATTCG GCAAAAATCT CTGAAGAAGA TCCACTCCAG AGCTTCAAGT 
GATGTCAGGC GAACGGCCTC ATCACGTCGT GGACGTCGCT CCGTACGTTC GGGTTATGCC 
TTTGCCCATC AGGAGGGCTT TGGTCGTCTG ATAACCTCCG GCAAAATTAT GCACAAGCTG 
CCGCAGGACT TTGCTTTTCC TCTGGGCTTG GGCACCAAGA AGACACAGGT GCTACACAAC 
AACCTGAACT CGGCCGATGG ACCAAATTCT AAGGGCAACA ATGTGACTGG CCATCATATG 
GTCAACAACA ACACAAATAT GCGACATAAT CAGAACCAAA ACCACTCGTC GATGGCAGAC 
ATAACGGCCG ATGGGCGGGG CAATGGGGGA GATGATGGCA GAGGAAGTGG AGGCTCAGAT 
GATATGAGTC CTCGTGCTCC CTGCCAGGAC CTGGATACGA TTAATCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8B ( NM_001300367.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287296.1 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant I, mRNA.

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CloneID	OFa22086
Clone ID Related Accession (Same CDS sequence)	NM_001300367.1
Accession Version	NM_001300367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG CCCTTCAAAG TGTTGACTTT TCGGCCAATC CGCATCACGA GAGTGACTTC 
CGCTGGTACG ATCGCACGTT GCTGGATGCC GTCCGGTCGG ATGAAGAGCA CTCCCATGTG 
TTTTTCCGCC TCCTGGCCCT CTGCCACACG GTCATGGCCG AAACGGTTGA TGGTAAGTTG 
GAGTACCAGG CCCAAAGTCC CGACGAAGCT GCCCTCGTTT CGGCCGCTCG AAATTTTGGC 
TTTGTCTTTC GCACACGAAC ACCGAATAGT ATTACCATCG AGGTGATGGG ACAAACGGAG 
GAGTACGAAC TCCTAAATAT CCTCGACTTT AACAACGTCC GCAAGCGGAT GTCTGTGATT 
CTCCGGCGCG GTGACTCCAT GGTTCTCTAC TGCAAAGGAG CGGACAATGT GATATATGAT 
CGTCTGCATG GCGGACAGGA GGACCTTAAG GCGCGCACCC AGGACCACCT TAATAAATTT 
GCCGGCGAAG GACTACGTAC TTTAGCGCTG GCTGAACGGC GTTTAACAGA GCAGTACTAC 
AACGACTGGA GGAGTCGGCA ACAGGAGGCA GCCCTTTCGA TGGACTCGAG GGAGCAGAAG 
CTGAATGCCA TTTACGAGGA GATCGAGAGC GAGATGCAGC TGGTCGGGGT GACCGCAATT 
GAGGACAAAC TGCAGGACGG CGTGCCCAAG TCAATTGCTA ATTTGCAGAA TGCAGGCATA 
AAGATATGGG TCCTAACCGG CGACAAGCAG GAAACTGCCA TCAATATCGG CTACTCCTGC 
CAACTGCTTA CGGATGAACT GGCGGATGTC TTTATCGTGG ACGGCAATTC GGTGGAGGAA 
GTGGAAAAGC AGCTAAGGCA GTTTAAGGAA TCTATTAAGA TTTATAACCG GTTTCGACCA 
GGCGGATTTG ATCCATTTGA TCGCTTAAAC AGCGACAGCA ACATGGATCC GCTAAGCGTG 
ACCATGACAC AAACATCGGC TTTCATGCAG GAGACGAACC TCCCGCCCAC GCCGCCTCCG 
CCGCCCGCTA TTTCGGTGGT TACCTTTAGT GCTGAGTGCA ATAACTTGTT CGGTGATGAA 
AAGGGAAGCG AAGATGGAGG CACTGCCTCC ATTGTGGTGG ATGAAAATAC AGGATTCGCT 
CTGGTGGTAA ATGGTCATTC CCTGGTGCAC TGTCTTTCGC CGGAATTGGA GAACAAATTC 
CTGGACATCG CCTCGCAGTG CAAGGCGGTC ATCTGTTGTC GGGTAACGCC ACTTCAGAAG 
GCGCTGGTCG TCGAGCTAAT AAAGCGTGCC AAAAACGCAG TCACTCTGGC CATTGGCGAT 
GGTGCCAACG ATGTGTCCAT GATTAAAGCT GCTCACATAG GCGTTGGAAT CTCAGGACAG 
GAGGGTCTTC AGGCTGTCCT CTCCAGCGAC TATTCCATTG CCCAGTTTCG ATACCTTGAG 
CGATTGTTAC TGGTACATGG TCGATGGTCC TACTACCGCA TGTGCAAGTT CCTCAGATAC 
TTTTTCTACA AGAACTTTGC ATTTACACTG TGCCATTGCT GGTATTCGCT CTTCTGCGGC 
TTCAGCGCTC AGACGGTTTT CGACCCGATG TTCATATCAG TGTATAATCT ATTTTACACA 
TCGCTACCCG TTTTGGCTTT GGGCGTCTTC GAACAGGATG TCTCGGACAA GAACAGTCTA 
GAGTTTCCGC GCCTCTACAC TCCGGGTCTA AAGAGCGAGT TGTTCAACAT TCGAGAGTTC 
ATCTACAGTG TGTTGCACGG CGCCTTCACC TCACTGGTCC TGTTCCTGAT TCCTTATGGC 
GTCTACAAGG ATGGCGTTTC GGCAAACGGA TTTGTTGTGA GCGATCACAT GACCCTGGGC 
GCTGTTGTCG CCACCATACT TATAGTGGAT AATACAGCGC AGATATCGTT GTACACCTCC 
TATTGGACCG TTGTGAATCA TGTGACCATT TGGGGTAGTC TAGTTTGGTA CTTTGTGCTG 
GACTACTTCT ACAACTATGT GATTGGTGGC CCTTACGTTG GCTCCTTGAC TCAAGCTATG 
AAGGACCTGA CCTTTTGGGT TACCATGTTG ATCACGGTGA TGGCGTTGGT GGCTCCTGTG 
TTGGCCTACA AGTTCTATTT GCTAGATGTA CATCCGAGCC TGTCGGATAA GATTCGGCAA 
AAATCTCTGA AGAAGATCCA CTCCAGAGCT TCAAGTGATG TCAGGCGAAC GGCCTCATCA 
CGTCGTGGAC GTCGCTCCGT ACGTTCGGGT TATGCCTTTG CCCATCAGGA GGGCTTTGGT 
CGTCTGATAA CCTCCGGCAA AATTATGCAC AAGCTGCCGC AGGACTTTGC TTTTCCTCTG 
GGCTTGGGCA CCAAGAAGAC ACAGGTGCTA CACAACAACC TGAACTCGGC CGATGGACCA 
AATTCTAAGG GCAACAATGT GACTGGCCAT CATATGGTCA ACAACAACAC AAATATGCGA 
CATAATCAGA ACCAAAACCA CTCGTCGATG GCAGACATAA CGGCCGATGG GCGGGGCAAT 
GGGGGAGATG ATGGCAGAGG AAGTGGAGGC TCAGATGATA TGAGTCCTCG TGCTCCCTGC 
CAGGACCTGG ATACGATTAA TCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287296.1
CDS	649..4995
Translation	MGTKTQPQLAKENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_001300367.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300367.1

ORF Insert Sequence:

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG CCCTTCAAAG TGTTGACTTT TCGGCCAATC CGCATCACGA GAGTGACTTC 
CGCTGGTACG ATCGCACGTT GCTGGATGCC GTCCGGTCGG ATGAAGAGCA CTCCCATGTG 
TTTTTCCGCC TCCTGGCCCT CTGCCACACG GTCATGGCCG AAACGGTTGA TGGTAAGTTG 
GAGTACCAGG CCCAAAGTCC CGACGAAGCT GCCCTCGTTT CGGCCGCTCG AAATTTTGGC 
TTTGTCTTTC GCACACGAAC ACCGAATAGT ATTACCATCG AGGTGATGGG ACAAACGGAG 
GAGTACGAAC TCCTAAATAT CCTCGACTTT AACAACGTCC GCAAGCGGAT GTCTGTGATT 
CTCCGGCGCG GTGACTCCAT GGTTCTCTAC TGCAAAGGAG CGGACAATGT GATATATGAT 
CGTCTGCATG GCGGACAGGA GGACCTTAAG GCGCGCACCC AGGACCACCT TAATAAATTT 
GCCGGCGAAG GACTACGTAC TTTAGCGCTG GCTGAACGGC GTTTAACAGA GCAGTACTAC 
AACGACTGGA GGAGTCGGCA ACAGGAGGCA GCCCTTTCGA TGGACTCGAG GGAGCAGAAG 
CTGAATGCCA TTTACGAGGA GATCGAGAGC GAGATGCAGC TGGTCGGGGT GACCGCAATT 
GAGGACAAAC TGCAGGACGG CGTGCCCAAG TCAATTGCTA ATTTGCAGAA TGCAGGCATA 
AAGATATGGG TCCTAACCGG CGACAAGCAG GAAACTGCCA TCAATATCGG CTACTCCTGC 
CAACTGCTTA CGGATGAACT GGCGGATGTC TTTATCGTGG ACGGCAATTC GGTGGAGGAA 
GTGGAAAAGC AGCTAAGGCA GTTTAAGGAA TCTATTAAGA TTTATAACCG GTTTCGACCA 
GGCGGATTTG ATCCATTTGA TCGCTTAAAC AGCGACAGCA ACATGGATCC GCTAAGCGTG 
ACCATGACAC AAACATCGGC TTTCATGCAG GAGACGAACC TCCCGCCCAC GCCGCCTCCG 
CCGCCCGCTA TTTCGGTGGT TACCTTTAGT GCTGAGTGCA ATAACTTGTT CGGTGATGAA 
AAGGGAAGCG AAGATGGAGG CACTGCCTCC ATTGTGGTGG ATGAAAATAC AGGATTCGCT 
CTGGTGGTAA ATGGTCATTC CCTGGTGCAC TGTCTTTCGC CGGAATTGGA GAACAAATTC 
CTGGACATCG CCTCGCAGTG CAAGGCGGTC ATCTGTTGTC GGGTAACGCC ACTTCAGAAG 
GCGCTGGTCG TCGAGCTAAT AAAGCGTGCC AAAAACGCAG TCACTCTGGC CATTGGCGAT 
GGTGCCAACG ATGTGTCCAT GATTAAAGCT GCTCACATAG GCGTTGGAAT CTCAGGACAG 
GAGGGTCTTC AGGCTGTCCT CTCCAGCGAC TATTCCATTG CCCAGTTTCG ATACCTTGAG 
CGATTGTTAC TGGTACATGG TCGATGGTCC TACTACCGCA TGTGCAAGTT CCTCAGATAC 
TTTTTCTACA AGAACTTTGC ATTTACACTG TGCCATTGCT GGTATTCGCT CTTCTGCGGC 
TTCAGCGCTC AGACGGTTTT CGACCCGATG TTCATATCAG TGTATAATCT ATTTTACACA 
TCGCTACCCG TTTTGGCTTT GGGCGTCTTC GAACAGGATG TCTCGGACAA GAACAGTCTA 
GAGTTTCCGC GCCTCTACAC TCCGGGTCTA AAGAGCGAGT TGTTCAACAT TCGAGAGTTC 
ATCTACAGTG TGTTGCACGG CGCCTTCACC TCACTGGTCC TGTTCCTGAT TCCTTATGGC 
GTCTACAAGG ATGGCGTTTC GGCAAACGGA TTTGTTGTGA GCGATCACAT GACCCTGGGC 
GCTGTTGTCG CCACCATACT TATAGTGGAT AATACAGCGC AGATATCGTT GTACACCTCC 
TATTGGACCG TTGTGAATCA TGTGACCATT TGGGGTAGTC TAGTTTGGTA CTTTGTGCTG 
GACTACTTCT ACAACTATGT GATTGGTGGC CCTTACGTTG GCTCCTTGAC TCAAGCTATG 
AAGGACCTGA CCTTTTGGGT TACCATGTTG ATCACGGTGA TGGCGTTGGT GGCTCCTGTG 
TTGGCCTACA AGTTCTATTT GCTAGATGTA CATCCGAGCC TGTCGGATAA GATTCGGCAA 
AAATCTCTGA AGAAGATCCA CTCCAGAGCT TCAAGTGATG TCAGGCGAAC GGCCTCATCA 
CGTCGTGGAC GTCGCTCCGT ACGTTCGGGT TATGCCTTTG CCCATCAGGA GGGCTTTGGT 
CGTCTGATAA CCTCCGGCAA AATTATGCAC AAGCTGCCGC AGGACTTTGC TTTTCCTCTG 
GGCTTGGGCA CCAAGAAGAC ACAGGTGCTA CACAACAACC TGAACTCGGC CGATGGACCA 
AATTCTAAGG GCAACAATGT GACTGGCCAT CATATGGTCA ACAACAACAC AAATATGCGA 
CATAATCAGA ACCAAAACCA CTCGTCGATG GCAGACATAA CGGCCGATGG GCGGGGCAAT 
GGGGGAGATG ATGGCAGAGG AAGTGGAGGC TCAGATGATA TGAGTCCTCG TGCTCCCTGC 
CAGGACCTGG ATACGATTAA TCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8B ( NM_001300366.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287295.1 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa22087
Clone ID Related Accession (Same CDS sequence)	NM_001300366.1
Accession Version	NM_001300366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG CCCTTCAAAG TGTTGACTTT TCGGCCAATC CGCATCACGA GAGTGACTTC 
CGCTGGTACG ATCGCACGTT GCTGGATGCC GTCCGGTCGG ATGAAGAGCA CTCCCATGTG 
TTTTTCCGCC TCCTGGCCCT CTGCCACACG GTCATGGCCG AAACGGTTGA TGGTAAGTTG 
GAGTACCAGG CCCAAAGTCC CGACGAAGCT GCCCTCGTTT CGGCCGCTCG AAATTTTGGC 
TTTGTCTTTC GCACACGAAC ACCGAATAGT ATTACCATCG AGGTGATGGG ACAAACGGAG 
GAGTACGAAC TCCTAAATAT CCTCGACTTT AACAACGTCC GCAAGCGGAT GTCTGTGATT 
CTCCGGCGCG GTGACTCCAT GGTTCTCTAC TGCAAAGGAG CGGACAATGT GATATATGAT 
CGTCTGCATG GCGGACAGGA GGACCTTAAG GCGCGCACCC AGGACCACCT TAATAAATTT 
GCCGGCGAAG GACTACGTAC TTTAGCGCTG GCTGAACGGC GTTTAACAGA GCAGTACTAC 
AACGACTGGA GGAGTCGGCA ACAGGAGGCA GCCCTTTCGA TGGACTCGAG GGAGCAGAAG 
CTGAATGCCA TTTACGAGGA GATCGAGAGC GAGATGCAGC TGGTCGGGGT GACCGCAATT 
GAGGACAAAC TGCAGGACGG CGTGCCCAAG TCAATTGCTA ATTTGCAGAA TGCAGGCATA 
AAGATATGGG TCCTAACCGG CGACAAGCAG GAAACTGCCA TCAATATCGG CTACTCCTGC 
CAACTGCTTA CGGATGAACT GGCGGATGTC TTTATCGTGG ACGGCAATTC GGTGGAGGAA 
GTGGAAAAGC AGCTAAGGCA GTTTAAGGAA TCTATTAAGA TTTATAACCG GTTTCGACCA 
GGCGGATTTG ATCCATTTGA TCGCTTAAAC AGCGACAGCA ACATGGATCC GCTAAGCGTG 
ACCATGACAC AAACATCGGC TTTCATGCAG GAGACGAACC TCCCGCCCAC GCCGCCTCCG 
CCGCCCGCTA TTTCGGTGGT TACCTTTAGG TGGGATGAGA AAAATAAGGA TAATAAGGGC 
GGACCGGACA GTGCTGAGTG CAATAACTTG TTCGGTGATG AAAAGGGAAG CGAAGATGGA 
GGCACTGCCT CCATTGTGGT GGATGAAAAT ACAGGATTCG CTCTGGTGGT AAATGGTCAT 
TCCCTGGTGC ACTGTCTTTC GCCGGAATTG GAGAACAAAT TCCTGGACAT CGCCTCGCAG 
TGCAAGGCGG TCATCTGTTG TCGGGTAACG CCACTTCAGA AGGCGCTGGT CGTCGAGCTA 
ATAAAGCGTG CCAAAAACGC AGTCACTCTG GCCATTGGCG ATGGTGCCAA CGATGTGTCC 
ATGATTAAAG CTGCTCACAT AGGCGTTGGA ATCTCAGGAC AGGAGGGTCT TCAGGCTGTC 
CTCTCCAGCG ACTATTCCAT TGCCCAGTTT CGATACCTTG AGCGATTGTT ACTGGTACAT 
GGTCGATGGT CCTACTACCG CATGTGCAAG TTCCTCAGAT ACTTTTTCTA CAAGAACTTT 
GCATTTACAC TGTGCCATTG CTGGTATTCG CTCTTCTGCG GCTTCAGCGC TCAGACGGTT 
TTCGACCCGA TGTTCATATC AGTGTATAAT CTATTTTACA CATCGCTACC CGTTTTGGCT 
TTGGGCGTCT TCGAACAGGA TGTCTCGGAC AAGAACAGTC TAGAGTTTCC GCGCCTCTAC 
ACTCCGGGTC TAAAGAGCGA GTTGTTCAAC ATTCGAGAGT TCATCTACAG TGTGTTGCAC 
GGCGCCTTCA CCTCACTGGT CCTGTTCCTG ATTCCTTATG GCGTCTACAA GGATGGCGTT 
TCGGCAAACG GATTTGTTGT GAGCGATCAC ATGACCCTGG GCGCTGTTGT CGCCACCATA 
CTTATAGTGG ATAATACAGC GCAGATATCG TTGTACACCT CCTATTGGAC CGTTGTGAAT 
CATGTGACCA TTTGGGGTAG TCTAGTTTGG TACTTTGTGC TGGACTACTT CTACAACTAT 
GTGATTGGTG GCCCTTACGT TGGCTCCTTG ACTCAAGCTA TGAAGGACCT GACCTTTTGG 
GTTACCATGT TGATCACGGT GATGGCGTTG GTGGCTCCTG TGTTGGCCTA CAAGTTCTAT 
TTGCTAGATG TACATCCGAG CCTGTCGGAT AAGATTCGGC AAAAATCTCT GAAGAAGATC 
CACTCCAGAG CTTCAAGTGA TGTCAGGCGA ACGGCCTCAT CACGTCGTGG ACGTCGCTCC 
GTACGTTCGG GTTATGCCTT TGCCCATCAG GAGGGCTTTG GTCGTCTGAT AACCTCCGGC 
AAAATTATGC ACAAGCTGCC GCAGGACTTT GCTTTTCCTC TGGGCTTGGG CACCAAGAAG 
ACACAGGTGC TACACAACAA CCTGAACTCG GCCGATGGAC CAAATTCTAA GGGCAACAAT 
GTGACTGGCC ATCATATGGT CAACAACAAC ACAAATATGC GACATAATCA GAACCAAAAC 
CACTCGTCGA TGGCAGACAT AACGGCCGAT GGGCGGGGCA ATGGGGGAGA TGATGGCAGA 
GGAAGTGGAG GCTCAGATGA TATGAGTCCT CGTGCTCCCT GCCAGGACCT GGATACGATT 
AATCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287295.1
CDS	649..5037
Translation	MGTKTQPQLAKENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_001300366.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300366.1

ORF Insert Sequence:

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG CCCTTCAAAG TGTTGACTTT TCGGCCAATC CGCATCACGA GAGTGACTTC 
CGCTGGTACG ATCGCACGTT GCTGGATGCC GTCCGGTCGG ATGAAGAGCA CTCCCATGTG 
TTTTTCCGCC TCCTGGCCCT CTGCCACACG GTCATGGCCG AAACGGTTGA TGGTAAGTTG 
GAGTACCAGG CCCAAAGTCC CGACGAAGCT GCCCTCGTTT CGGCCGCTCG AAATTTTGGC 
TTTGTCTTTC GCACACGAAC ACCGAATAGT ATTACCATCG AGGTGATGGG ACAAACGGAG 
GAGTACGAAC TCCTAAATAT CCTCGACTTT AACAACGTCC GCAAGCGGAT GTCTGTGATT 
CTCCGGCGCG GTGACTCCAT GGTTCTCTAC TGCAAAGGAG CGGACAATGT GATATATGAT 
CGTCTGCATG GCGGACAGGA GGACCTTAAG GCGCGCACCC AGGACCACCT TAATAAATTT 
GCCGGCGAAG GACTACGTAC TTTAGCGCTG GCTGAACGGC GTTTAACAGA GCAGTACTAC 
AACGACTGGA GGAGTCGGCA ACAGGAGGCA GCCCTTTCGA TGGACTCGAG GGAGCAGAAG 
CTGAATGCCA TTTACGAGGA GATCGAGAGC GAGATGCAGC TGGTCGGGGT GACCGCAATT 
GAGGACAAAC TGCAGGACGG CGTGCCCAAG TCAATTGCTA ATTTGCAGAA TGCAGGCATA 
AAGATATGGG TCCTAACCGG CGACAAGCAG GAAACTGCCA TCAATATCGG CTACTCCTGC 
CAACTGCTTA CGGATGAACT GGCGGATGTC TTTATCGTGG ACGGCAATTC GGTGGAGGAA 
GTGGAAAAGC AGCTAAGGCA GTTTAAGGAA TCTATTAAGA TTTATAACCG GTTTCGACCA 
GGCGGATTTG ATCCATTTGA TCGCTTAAAC AGCGACAGCA ACATGGATCC GCTAAGCGTG 
ACCATGACAC AAACATCGGC TTTCATGCAG GAGACGAACC TCCCGCCCAC GCCGCCTCCG 
CCGCCCGCTA TTTCGGTGGT TACCTTTAGG TGGGATGAGA AAAATAAGGA TAATAAGGGC 
GGACCGGACA GTGCTGAGTG CAATAACTTG TTCGGTGATG AAAAGGGAAG CGAAGATGGA 
GGCACTGCCT CCATTGTGGT GGATGAAAAT ACAGGATTCG CTCTGGTGGT AAATGGTCAT 
TCCCTGGTGC ACTGTCTTTC GCCGGAATTG GAGAACAAAT TCCTGGACAT CGCCTCGCAG 
TGCAAGGCGG TCATCTGTTG TCGGGTAACG CCACTTCAGA AGGCGCTGGT CGTCGAGCTA 
ATAAAGCGTG CCAAAAACGC AGTCACTCTG GCCATTGGCG ATGGTGCCAA CGATGTGTCC 
ATGATTAAAG CTGCTCACAT AGGCGTTGGA ATCTCAGGAC AGGAGGGTCT TCAGGCTGTC 
CTCTCCAGCG ACTATTCCAT TGCCCAGTTT CGATACCTTG AGCGATTGTT ACTGGTACAT 
GGTCGATGGT CCTACTACCG CATGTGCAAG TTCCTCAGAT ACTTTTTCTA CAAGAACTTT 
GCATTTACAC TGTGCCATTG CTGGTATTCG CTCTTCTGCG GCTTCAGCGC TCAGACGGTT 
TTCGACCCGA TGTTCATATC AGTGTATAAT CTATTTTACA CATCGCTACC CGTTTTGGCT 
TTGGGCGTCT TCGAACAGGA TGTCTCGGAC AAGAACAGTC TAGAGTTTCC GCGCCTCTAC 
ACTCCGGGTC TAAAGAGCGA GTTGTTCAAC ATTCGAGAGT TCATCTACAG TGTGTTGCAC 
GGCGCCTTCA CCTCACTGGT CCTGTTCCTG ATTCCTTATG GCGTCTACAA GGATGGCGTT 
TCGGCAAACG GATTTGTTGT GAGCGATCAC ATGACCCTGG GCGCTGTTGT CGCCACCATA 
CTTATAGTGG ATAATACAGC GCAGATATCG TTGTACACCT CCTATTGGAC CGTTGTGAAT 
CATGTGACCA TTTGGGGTAG TCTAGTTTGG TACTTTGTGC TGGACTACTT CTACAACTAT 
GTGATTGGTG GCCCTTACGT TGGCTCCTTG ACTCAAGCTA TGAAGGACCT GACCTTTTGG 
GTTACCATGT TGATCACGGT GATGGCGTTG GTGGCTCCTG TGTTGGCCTA CAAGTTCTAT 
TTGCTAGATG TACATCCGAG CCTGTCGGAT AAGATTCGGC AAAAATCTCT GAAGAAGATC 
CACTCCAGAG CTTCAAGTGA TGTCAGGCGA ACGGCCTCAT CACGTCGTGG ACGTCGCTCC 
GTACGTTCGG GTTATGCCTT TGCCCATCAG GAGGGCTTTG GTCGTCTGAT AACCTCCGGC 
AAAATTATGC ACAAGCTGCC GCAGGACTTT GCTTTTCCTC TGGGCTTGGG CACCAAGAAG 
ACACAGGTGC TACACAACAA CCTGAACTCG GCCGATGGAC CAAATTCTAA GGGCAACAAT 
GTGACTGGCC ATCATATGGT CAACAACAAC ACAAATATGC GACATAATCA GAACCAAAAC 
CACTCGTCGA TGGCAGACAT AACGGCCGAT GGGCGGGGCA ATGGGGGAGA TGATGGCAGA 
GGAAGTGGAG GCTCAGATGA TATGAGTCCT CGTGCTCCCT GCCAGGACCT GGATACGATT 
AATCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8B ( NM_001260140.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247069.2 Drosophila melanogaster ATPase 8B (ATP8B), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa22088
Clone ID Related Accession (Same CDS sequence)	NM_001260140.2
Accession Version	NM_001260140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATPase 8B, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260140.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG TAAAGATTTT GCATGACAGC CAACAGACAG AAACACACAA CATGCACCAT 
TCAAGTCACA ATCGCAAGCG ATTAGTCAAG ATCCGGTTTA AAAAAGCGCC ATCAACGGCC 
ACGCTGGGTG TTGTCATCTC CCAGCTCGAG CACCACCTCG ACGAGGAGCC ACAATCCACA 
TCCACACTTA GCACCACCAA GCACCACCAC CATCGATCCA AGGCATTCGC CACCAATTGG 
AGTTCGTCGC CTCACAGAGT GCACGCCCTT CAAAGTGTTG ACTTTTCGGC CAATCCGCAT 
CACGAGAGTG ACTTCCGCTG GTACGATCGC ACGTTGCTGG ATGCCGTCCG GTCGGATGAA 
GAGCACTCCC ATGTGTTTTT CCGCCTCCTG GCCCTCTGCC ACACGGTCAT GGCCGAAACG 
GTTGATGGTA AGTTGGAGTA CCAGGCCCAA AGTCCCGACG AAGCTGCCCT CGTTTCGGCC 
GCTCGAAATT TTGGCTTTGT CTTTCGCACA CGAACACCGA ATAGTATTAC CATCGAGGTG 
ATGGGACAAA CGGAGGAGTA CGAACTCCTA AATATCCTCG ACTTTAACAA CGTCCGCAAG 
CGGATGTCTG TGATTCTCCG GCGCGGTGAC TCCATGGTTC TCTACTGCAA AGGAGCGGAC 
AATGTGATAT ATGATCGTCT GCATGGCGGA CAGGAGGACC TTAAGGCGCG CACCCAGGAC 
CACCTTAATA AATTTGCCGG CGAAGGACTA CGTACTTTAG CGCTGGCTGA ACGGCGTTTA 
ACAGAGCAGT ACTACAACGA CTGGAGGAGT CGGCAACAGG AGGCAGCCCT TTCGATGGAC 
TCGAGGGAGC AGAAGCTGAA TGCCATTTAC GAGGAGATCG AGAGCGAGAT GCAGCTGGTC 
GGGGTGACCG CAATTGAGGA CAAACTGCAG GACGGCGTGC CCAAGTCAAT TGCTAATTTG 
CAGAATGCAG GCATAAAGAT ATGGGTCCTA ACCGGCGACA AGCAGGAAAC TGCCATCAAT 
ATCGGCTACT CCTGCCAACT GCTTACGGAT GAACTGGCGG ATGTCTTTAT CGTGGACGGC 
AATTCGGTGG AGGAAGTGGA AAAGCAGCTA AGGCAGTTTA AGGAATCTAT TAAGATTTAT 
AACCGGTTTC GACCAGGCGG ATTTGATCCA TTTGATCGCT TAAACAGCGA CAGCAACATG 
GATCCGCTAA GCGTGACCAT GACACAAACA TCGGCTTTCA TGCAGGAGAC GAACCTCCCG 
CCCACGCCGC CTCCGCCGCC CGCTATTTCG GTGGTTACCT TTAGTGCTGA GTGCAATAAC 
TTGTTCGGTG ATGAAAAGGG AAGCGAAGAT GGAGGCACTG CCTCCATTGT GGTGGATGAA 
AATACAGGAT TCGCTCTGGT GGTAAATGGT CATTCCCTGG TGCACTGTCT TTCGCCGGAA 
TTGGAGAACA AATTCCTGGA CATCGCCTCG CAGTGCAAGG CGGTCATCTG TTGTCGGGTA 
ACGCCACTTC AGAAGGCGCT GGTCGTCGAG CTAATAAAGC GTGCCAAAAA CGCAGTCACT 
CTGGCCATTG GCGATGGTGC CAACGATGTG TCCATGATTA AAGCTGCTCA CATAGGCGTT 
GGAATCTCAG GACAGGAGGG TCTTCAGGCT GTCCTCTCCA GCGACTATTC CATTGCCCAG 
TTTCGATACC TTGAGCGATT GTTACTGGTA CATGGTCGAT GGTCCTACTA CCGCATGTGC 
AAGTTCCTCA GATACTTTTT CTACAAGAAC TTTGCATTTA CACTGTGCCA TTGCTGGTAT 
TCGCTCTTCT GCGGCTTCAG CGCTCAGACG GTTTTCGACC CGATGTTCAT ATCAGTGTAT 
AATCTATTTT ACACATCGCT ACCCGTTTTG GCTTTGGGCG TCTTCGAACA GGATGTCTCG 
GACAAGAACA GTCTAGAGTT TCCGCGCCTC TACACTCCGG GTCTAAAGAG CGAGTTGTTC 
AACATTCGAG AGTTCATCTA CAGTGTGTTG CACGGCGCCT TCACCTCACT GGTCCTGTTC 
CTGATTCCTT ATGGCGTCTA CAAGGATGGC GTTTCGGCAA ACGGATTTGT TGTGAGCGAT 
CACATGACCC TGGGCGCTGT TGTCGCCACC ATACTTATAG TGGATAATAC AGCGCAGATA 
TCGTTGTACA CCTCCTATTG GACCGTTGTG AATCATGTGA CCATTTGGGG TAGTCTAGTT 
TGGTACTTTG TGCTGGACTA CTTCTACAAC TATGTGATTG GTGGCCCTTA CGTTGGCTCC 
TTGACTCAAG CTATGAAGGA CCTGACCTTT TGGGTTACCA TGTTGATCAC GGTGATGGCG 
TTGGTGGCTC CTGTGTTGGC CTACAAGTTC TATTTGCTAG ATGTACATCC GAGCCTGTCG 
GATAAGATTC GGCAAAAATC TCTGAAGAAG ATCCACTCCA GAGCTTCAAG TGATGTCAGG 
CGAACGGCCT CATCACGTCG TGGACGTCGC TCCGTACGTT CGGGTTATGC CTTTGCCCAT 
CAGGAGGGCT TTGGTCGTCT GATAACCTCC GGCAAAATTA TGCACAAGCT GCCGCAGGAC 
TTTGCTTTTC CTCTGGGCTT GGGCACCAAG AAGACACAGG TGCTACACAA CAACCTGAAC 
TCGGCCGATG GACCAAATTC TAAGGGCAAC AATGTGACTG GCCATCATAT GGTCAACAAC 
AACACAAATA TGCGACATAA TCAGAACCAA AACCACTCGT CGATGGCAGA CATAACGGCC 
GATGGGCGGG GCAATGGGGG AGATGATGGC AGAGGAAGTG GAGGCTCAGA TGATATGAGT 
CCTCGTGCTC CCTGCCAGGA CCTGGATACG ATTAATCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247069.2
CDS	649..5250
Translation	MGTKTQPQLAKENERRIRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGTLIWRGQRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNFIIIGIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTGATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKVKILHDSQQTETHNMHHSSHNRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTSTLSTTKHHHHRSKAFATNWSSSPHRVHALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVILRRGDSMVLYCKGADNVIYDRLHGGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLSPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWYFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSMADITADGRGNGGDDGRGSGGSDDMSPRAPCQDLDTINL

Target ORF information:

RefSeq Version	NM_001260140.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATPase 8B (ATP8B), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260140.2

ORF Insert Sequence:

ATGGGCACAA AAACCCAACC ACAATTGGCC AAAGAAAACG AACGTAGAAT TCGAGCCAAC 
GATAAAGAGT TCAATGCCCA GTTCAAATAT CATAACAACT ACATCAAGAC CTCCAAGTAT 
TCGCTGTTCA CGTTTCTGCC ATTCAATCTG CTGGAGCAAT TTCAGCGGCT GGCCAACTTC 
TATTTCCTCT GCCTGCTGGT CCTCCAGCTG ATACCCGCGA TATCCTCACT CACCCCAGTG 
ACCACAGCAA TTCCCCTGAT AGGAGTACTT ACTCTGACGG CTGTTAAGGA TGCGTATGAT 
GATATTCAAC GTCATATTTC TGACTCGCAG GTAAACAATC GCAAGTCGAA AACGCTGCGC 
AATGGCAAGT TGGTGGAGGC CAAGTGGTCG GAGGTACAGG TGGGCGATGT CATTCGGCTG 
GACAACAATC AGTTCGTGGC CGCCGACACC CTGTTGCTGT CCACATCCGA GCCAAATGGT 
CTGTGTTTCA TCGAGACAGC CGAACTGGAT GGGGAGACGA ATCTCAAGGC AAAGCAATGC 
CTGACCGAAA CCATCGAGCT GGGTGATCGC CATGACTCGC TATGGAACTT CAATGGCGAG 
ATCATTTGCG AGCGGCCCAA CAACCTGCTG AACAAATTCG ATGGCACCTT GATTTGGCGG 
GGCCAGAGAT TCGCCCTGGA CAACGAGAAG ATCCTGCTGA GGGGCTGCGT CCTCCGGAAC 
ACGCAGTGGT GCTATGGCGT GGTGGTCTTC GCCGGAGTGG ACACCAAGTT GATGCAGAAC 
TCCGGAAAGA CGCAGTTCAA GAGCACTGGC GTGGATCGCC TGCTCAACTT TATTATCATT 
GGGATCGTCC TCTTTCTGGT GTCGATTTGT GCCCTTTTTG CGATTGGCTG TGCCATCTGG 
GAGGGCCTGA TTGGCCAGCA CTTCCAGCTG TATCTGCCTT GGGAGCACAT CATACCCAAA 
GATTACATAC CCACGGGAGC AACAGTCATT GGATTGCTGG TCTTCTTCTC GTATGCCATA 
GTCTTAAACA CAGTTGTGCC AATCTCACTC TACGTTTCAG TAGAGGTTAT ACGCTTTGTA 
CAGTCGTTCC TCATCAACTG GGATGAGGAG ATGTACTATC CGACCACCAA TACCTATGCC 
AAAGCCCGCA CCACCACGCT CAACGAGGAG CTGGGCCAGA TCCAGTACAT ATTCTCGGAT 
AAAACGGGCA CCCTCACCCA GAACATCATG ACCTTCAACA AGTGCAGCAT CAATGGGCGC 
AGCTATGGCG ATGTGATTGA CCTGCGCACC GGCGAGTTGG TCGAGATTAC GGAGCAGCAA 
ACAATTTTCC AAAATAGCAA CACCAACAAC AGACCCAGCC CCTTAAGTGG AGCTATCGTA 
GCACCACCTG CAGCACCACC CCCCATCATC CTAGTGCACA AGGCCGAGGT CCATGCGAAG 
AAGACTTCCA TGGTGGTCAC ATCCAGCGGG GAGGCGCAAG TGCTGCCAGA CAGACCCAGA 
TCGGATATCG AGCGCTCTGC TCCGCCGATG GACGCCAGCG AGAAGCGGCC GGGACTCAAG 
CATGTGCGAT ACTCGGCTCC CAGTAGAAGT CAGGATGCAG ACGCTGGTCG TTTGTCACCC 
AGGTTGGATG GAAGTGGAGG ACTTAGTCCA CCCGTAGGCA ATGAGGAGCG ACGAATTAGT 
GGCGGCTTCA AAAGGAGTGG AGCCGGATGT ATGCAGCGCC AATTGTCCCG CACTAGCAGT 
TGCGACAAAG TAAAGATTTT GCATGACAGC CAACAGACAG AAACACACAA CATGCACCAT 
TCAAGTCACA ATCGCAAGCG ATTAGTCAAG ATCCGGTTTA AAAAAGCGCC ATCAACGGCC 
ACGCTGGGTG TTGTCATCTC CCAGCTCGAG CACCACCTCG ACGAGGAGCC ACAATCCACA 
TCCACACTTA GCACCACCAA GCACCACCAC CATCGATCCA AGGCATTCGC CACCAATTGG 
AGTTCGTCGC CTCACAGAGT GCACGCCCTT CAAAGTGTTG ACTTTTCGGC CAATCCGCAT 
CACGAGAGTG ACTTCCGCTG GTACGATCGC ACGTTGCTGG ATGCCGTCCG GTCGGATGAA 
GAGCACTCCC ATGTGTTTTT CCGCCTCCTG GCCCTCTGCC ACACGGTCAT GGCCGAAACG 
GTTGATGGTA AGTTGGAGTA CCAGGCCCAA AGTCCCGACG AAGCTGCCCT CGTTTCGGCC 
GCTCGAAATT TTGGCTTTGT CTTTCGCACA CGAACACCGA ATAGTATTAC CATCGAGGTG 
ATGGGACAAA CGGAGGAGTA CGAACTCCTA AATATCCTCG ACTTTAACAA CGTCCGCAAG 
CGGATGTCTG TGATTCTCCG GCGCGGTGAC TCCATGGTTC TCTACTGCAA AGGAGCGGAC 
AATGTGATAT ATGATCGTCT GCATGGCGGA CAGGAGGACC TTAAGGCGCG CACCCAGGAC 
CACCTTAATA AATTTGCCGG CGAAGGACTA CGTACTTTAG CGCTGGCTGA ACGGCGTTTA 
ACAGAGCAGT ACTACAACGA CTGGAGGAGT CGGCAACAGG AGGCAGCCCT TTCGATGGAC 
TCGAGGGAGC AGAAGCTGAA TGCCATTTAC GAGGAGATCG AGAGCGAGAT GCAGCTGGTC 
GGGGTGACCG CAATTGAGGA CAAACTGCAG GACGGCGTGC CCAAGTCAAT TGCTAATTTG 
CAGAATGCAG GCATAAAGAT ATGGGTCCTA ACCGGCGACA AGCAGGAAAC TGCCATCAAT 
ATCGGCTACT CCTGCCAACT GCTTACGGAT GAACTGGCGG ATGTCTTTAT CGTGGACGGC 
AATTCGGTGG AGGAAGTGGA AAAGCAGCTA AGGCAGTTTA AGGAATCTAT TAAGATTTAT 
AACCGGTTTC GACCAGGCGG ATTTGATCCA TTTGATCGCT TAAACAGCGA CAGCAACATG 
GATCCGCTAA GCGTGACCAT GACACAAACA TCGGCTTTCA TGCAGGAGAC GAACCTCCCG 
CCCACGCCGC CTCCGCCGCC CGCTATTTCG GTGGTTACCT TTAGTGCTGA GTGCAATAAC 
TTGTTCGGTG ATGAAAAGGG AAGCGAAGAT GGAGGCACTG CCTCCATTGT GGTGGATGAA 
AATACAGGAT TCGCTCTGGT GGTAAATGGT CATTCCCTGG TGCACTGTCT TTCGCCGGAA 
TTGGAGAACA AATTCCTGGA CATCGCCTCG CAGTGCAAGG CGGTCATCTG TTGTCGGGTA 
ACGCCACTTC AGAAGGCGCT GGTCGTCGAG CTAATAAAGC GTGCCAAAAA CGCAGTCACT 
CTGGCCATTG GCGATGGTGC CAACGATGTG TCCATGATTA AAGCTGCTCA CATAGGCGTT 
GGAATCTCAG GACAGGAGGG TCTTCAGGCT GTCCTCTCCA GCGACTATTC CATTGCCCAG 
TTTCGATACC TTGAGCGATT GTTACTGGTA CATGGTCGAT GGTCCTACTA CCGCATGTGC 
AAGTTCCTCA GATACTTTTT CTACAAGAAC TTTGCATTTA CACTGTGCCA TTGCTGGTAT 
TCGCTCTTCT GCGGCTTCAG CGCTCAGACG GTTTTCGACC CGATGTTCAT ATCAGTGTAT 
AATCTATTTT ACACATCGCT ACCCGTTTTG GCTTTGGGCG TCTTCGAACA GGATGTCTCG 
GACAAGAACA GTCTAGAGTT TCCGCGCCTC TACACTCCGG GTCTAAAGAG CGAGTTGTTC 
AACATTCGAG AGTTCATCTA CAGTGTGTTG CACGGCGCCT TCACCTCACT GGTCCTGTTC 
CTGATTCCTT ATGGCGTCTA CAAGGATGGC GTTTCGGCAA ACGGATTTGT TGTGAGCGAT 
CACATGACCC TGGGCGCTGT TGTCGCCACC ATACTTATAG TGGATAATAC AGCGCAGATA 
TCGTTGTACA CCTCCTATTG GACCGTTGTG AATCATGTGA CCATTTGGGG TAGTCTAGTT 
TGGTACTTTG TGCTGGACTA CTTCTACAAC TATGTGATTG GTGGCCCTTA CGTTGGCTCC 
TTGACTCAAG CTATGAAGGA CCTGACCTTT TGGGTTACCA TGTTGATCAC GGTGATGGCG 
TTGGTGGCTC CTGTGTTGGC CTACAAGTTC TATTTGCTAG ATGTACATCC GAGCCTGTCG 
GATAAGATTC GGCAAAAATC TCTGAAGAAG ATCCACTCCA GAGCTTCAAG TGATGTCAGG 
CGAACGGCCT CATCACGTCG TGGACGTCGC TCCGTACGTT CGGGTTATGC CTTTGCCCAT 
CAGGAGGGCT TTGGTCGTCT GATAACCTCC GGCAAAATTA TGCACAAGCT GCCGCAGGAC 
TTTGCTTTTC CTCTGGGCTT GGGCACCAAG AAGACACAGG TGCTACACAA CAACCTGAAC 
TCGGCCGATG GACCAAATTC TAAGGGCAAC AATGTGACTG GCCATCATAT GGTCAACAAC 
AACACAAATA TGCGACATAA TCAGAACCAA AACCACTCGT CGATGGCAGA CATAACGGCC 
GATGGGCGGG GCAATGGGGG AGATGATGGC AGAGGAAGTG GAGGCTCAGA TGATATGAGT 
CCTCGTGCTC CCTGCCAGGA CCTGGATACG ATTAATCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ATP8B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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