Bruce cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bruce
Entrez Gene ID	41260
Full Name	BIR repeat containing ubiquitin-conjugating enzyme
Synonyms	BRUCE,CG6303,Dmel\CG6303,bruce,dBRUCE,dBruce,dbruce,mod86
General protein information	Preferred Names BIR repeat containing ubiquitin-conjugating enzyme Names CG6303 gene product from transcript CG6303-RD Bruce-PA Bruce-PB Bruce-PC Bruce-PD Bruce-PE CG6303-PA CG6303-PB CG6303-PC CG6303-PD CG6303-PE dbruce mod86
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86A7-86A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_141738.5	NP_649995.2	BIR repeat containing ubiquitin-conjugating enzyme, isoform A
NM_001275531.2	NP_001262460.1	BIR repeat containing ubiquitin-conjugating enzyme, isoform B
NM_001275533.2	NP_001262462.1	BIR repeat containing ubiquitin-conjugating enzyme, isoform D
NM_001275534.2	NP_001262463.1	BIR repeat containing ubiquitin-conjugating enzyme, isoform E
NM_001275532.2	NP_001262461.1	BIR repeat containing ubiquitin-conjugating enzyme, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BIRC6	XP_003309025.1
Gallus gallus (chicken)	BIRC6	XP_419512.4
Homo sapiens (human)	BIRC6	NP_057336.3
Drosophila melanogaster (fruit fly)	Bruce	NP_001262460.1
Canis lupus familiaris (dog)	BIRC6	XP_005630657.1
Rattus norvegicus (Norway rat)	Birc6	NP_001164067.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002651	XP_312274.4
Bos taurus (cattle)	BIRC6	NP_001193511.1
Mus musculus (house mouse)	Birc6	NP_031592.3
Xenopus tropicalis (tropical clawed frog)	birc6	XP_002934505.2
Danio rerio (zebrafish)	birc6	XP_001336902.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dBruce physically interacts with Reaper, dependent upon Reaper's IAP-binding (IBM) and GH3 motifs.
Title: Drosophila BRUCE inhibits apoptosis through non-lysine ubiquitination of the IAP-antagonist REAPER.

Drosophila inhibitor of apoptosis dBruce is degraded by autophagy and this degradation promotes DNA fragmentation and subsequent nurse cell death.
Title: Autophagy as a trigger for cell death: autophagic degradation of inhibitor of apoptosis dBruce controls DNA fragmentation during late oogenesis in Drosophila.

Results reveal autophagic degradation of the IAP protein Bruce as a novel mechanism of triggering cell death and thereby provide a mechanistic link between autophagy and cell death.
Title: Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis.

Data show that the effector caspase Dcp-1 and the inhibitor of apoptosis protein Bruce function to regulate both autophagy and starvation-induced cell death .
Title: Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesis.

Drosophila Bruce can potently suppress Rpr- and Grim-dependent but not Hid-dependent cell death
Title: Drosophila Bruce can potently suppress Rpr- and Grim-dependent but not Hid-dependent cell death.

The following Bruce gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bruce cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04451	NM_141738.5 Latest version!	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08255	NM_001275533.2 Latest version!	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08256	NM_001275532.2 Latest version!	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08257	NM_001275534.2 Latest version!	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08258	NM_001275531.2 Latest version!	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bruce ( NM_141738.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649995.2 Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04451
Clone ID Related Accession (Same CDS sequence)	NM_141738.5
Accession Version	NM_141738.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BIR repeat containing ubiquitin-conjugating enzyme, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141738.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCACTGCTT CCTCGTGTAC TGAGGCACTA 
TTTGAGACGC TGATGCCGAC AGCAAGAGAA TTTTATCAAG AGGCCGTCAA ATATAATTAC 
CTACCTTCAT TAATTGAATG CGATGACACG GAATTCGATG AAATGCTAAA CGACATTGAC 
ATGATTGAAC GAACGAAGCC AGTTACCAAA AAGGAAAGTA ATGCCCTGTC TAAGAATACA 
TTCAATTTCT TCTGCAAGTC TATCTCGATT GCTCTGGATT CACGATTGGA CGTGGGTCTG 
GAATTGCACG TAGAAACTCA GCTGAGAAGG CTAACAAATC GCACATCCCT GGATCTCTAT 
TCATCGCTTC CTCAGGTGTT GACCAATGAG TTTGTCTCTC CACCACCTGA GGCTGCTCCT 
TGGCCAGAAT ATTTGACTCA ACTTTGGTCA GGCACGGAAT ACAACGGTGA CAATACTTAT 
GTGATGTTTA AAAGGGTCTT TGACTCCATC CTGGCGGAAT TGCACCTTGA AGACACGTGG 
GTGCACTTGG AGCAGGTGCT GCAGATGTGG CTGACGCTAA ATTGCGAGCT CTCGGACAAG 
CCATACACCA GTGGCATCAC TCACTCGGAT GTTCCAAAAA TTCCGTTTGG AGCAAATGCG 
GTGCAGGGAT TGCTCCTGGC TTTGGCTTGG CACAGTGATA TAAAACTGCG GACCTGGTGT 
CTTGGCTTTC AGTGCCTGTT TCTCGCTTGC AACTCATTGC CTATCGGAGA TGATGCGGAT 
TGTACGCGTA TCAACGAGGC TATCGTGAAT GATGAAAACT TTGAAAAAGT GCTGCTACGA 
TTCTTTTCCG GTTATGGCAT GAGCTCATCT ATCATCACCA ACCGATGCGC TGGACCTACT 
ATTTGCAAGC ACTTGCATGA GTTATTGCTC TGGCTGCACA GCAAAAGCGA GACGAGTGGC 
ATTCCGTGCA AGCGTCGACT TAAGGACATT TTGTTGCATG TTGTGCTTCA GTTGGTGCAG 
CCTGGTGGCT CTATTAGCAA CCAGCAGGGT CCAATTGATG CTCAAAACCA GCTGGTTCGT 
GATTTGCTTA TGATGCCCAA TGATAAGGGG GACCTCAACA TTGCGCTTAA AATCACAGAA 
AGTGTTTCTT TCCTGGTGTA CAACAACATA TCCAATGGCG AGAGGCTACA ATGTCAGCGT 
GGAAATGAAA ACAGCCCAGT TGGCAACAAT TTTAGCAATT TGTTTGCCAA CGTTCTGGGA 
TCAGAAAATC CCAACAAGCA GAATGCCGCA ATTTGCGACA ACTCACTTAT TATAAATCTG 
CTGAAACTAT CGTCACACAT GGTATTGACA GAGATGCCCC GGCAACCAAG TGAGGTTACC 
ATTCCCGAGG AGGAGGAACT TTCCAATCAG AGTCAAACGG ATGAGACTAA AGCGGAGCAG 
TTAAACACGG AAGGACATCG CAGCAAAGTG CCTTGCATTG CAGATTGGGT ACTCCGCCAT 
TTCCCCACAA TGAAGCGACT TTTTGGAACG CTTTCCCAGT GCTCGACAAA CACCTTCACC 
ATGATGTCCT CTGGCTGTTT CTTTTCACCG AAGTCCAATA TGATTTTAGA TGACCCGCAG 
ACAATTGCAG ACGCCGTCTT TAGTTTGATG ATTACTCTCA ACGACAAGGC TTCGCATCCC 
CGACTTGTTG TGGTGCCCAT TTGCCGATAC CTTGAAGAAA CCACTATTCC GAGAAATGCA 
ACGCTGCCGC GTCTGCCGCA TTCTGAACCT TTCCTGTGGT ACATCTCGAA GGTATTGGAA 
GTACCAGCGG CAGTGCAAAC GTTCACCCAA TTGGGCGGAA TTCAGATTAT ATGTCATAGC 
CTGGTGCGAC TTAATAAAAC TATCATCAAC ATGCAACCAG GATTGATTTC TTTGATTATG 
CAACACTTGA CGCGCAATAC GCGCTTTAAG CGTAATAATC ATGTGGCGGC AAGTAGTGCT 
TGCAAAAAGA CAACGACTGG TACGGGAACC ACATGTCCCA CGTCAGCACA GCCGCCGAGA 
AGTGCTGCTC AGTACGATGG TCTGATCAAC TTTGCACCCT TCTCGCATAT TGTCTCCGAA 
AATGATGCGA CACAAAGTGC AGAGGTACTA ATATCAAATC CAAATAATTC ACATCGAAGA 
CCGCGATCGC CAGCCTGGAC CTATATGTTC TATCCAAATG AGACGCATGT GGATCTGACT 
ATAACGTTGC CCACAGCAAT TTTGTTGAAA GAGGTGCAGC TGGTGCCGCA CACAACTAGT 
TTGGCATCCT GTCCATCTGC GGTGGCTCTA GAGCTGTCTA GAGATTACGG CCTTGGTTCG 
ATCCCAGTGG GTGCTCCCAT GGCCACCACT GGTCTGACCT GCATTAGACT AAAGCTGGCC 
AAGGCAGAGG TGGCTACCAG CATTGTTCTG AGGCTGTACA AACCCAAGGA CTGTGGAAAT 
GTGGGTCTCG TACAAATCGC TGTGCTTGGT CAGACAATCT TCGGAAATCG CATGCATGGC 
CTTCGCTCAC CGAGTCCGTT CATTGACTCC CTAGCAACGT GCTCCTCGCC AGTGCAAACG 
GCAGTTTTAG CCGCAATGGA TGCGGATTCC ATGCTTGAAG ATGATGCTTT TGCGAAGACT 
AGCATTGGCT GGGTTCGCAT TCTGTCGCGC TGTTTCCATG TGGCCGCTTT ACAACCAGAC 
AGTAAGCTGG CTGATCTTAT CGCCGCCTAT CCGGCTGCAT ATCCCGGCTT TTTAGAGGCC 
TGCTGCTCAT TGCTAAACAT AATGCCAATG ATTCCTAGTC TTGCACAGCA ACACTTGGAG 
ACCATTTTGT TAAAATTAAG TGCATACAAC CACGAGATCA GTTTGCAACT TCTTAGGACG 
CTCCTGTGGC ACACCACTCC GCAAGTTTTT AAACTCTCCA GCGATTCGGT GTGTGATCTT 
ATTTATGAAG TAGTTACCGG ATCGACGGAG CACCTACTGG ACAAACTACG CGTGCTTATC 
GACTGGGTAA TGCAATTGCA CGACAACTAC AGCAAGCAGG CGCGCTGTAA TAACCCACAA 
AGTGGATTTG TTAAGGTGAT TGCTTCGATT CTGTGGAAAG TGCATACCCA GCAACAATTG 
CCTGAGTTAC CGCTTCTCAT TTCATCTTCG CTGTTCGAGA CCTGTTTTGA ATGGGTCTCT 
TCGCTGGAGC ACGATGAGCC ACTGAAGAGC AGTTTTGACT CCTTGTTGTG TGCATTGTGC 
TTCATTAAGC CAGAACTGTT TAACCAGCTG TTGGTCAAGC TGGGAGTTAA GCTGGAACCT 
CCAGCTCGAG GTCAAACTGA TGACAGCAAA GTGCAAAGTG GTAGTGCTTG GTTCCGCCGT 
GAGGGTAGCG AAAACCTTAG TGTTTTGCTA CAGCGTCCCA CATTCCTAAA GACTCTAGCC 
TTGGCCTGCC AGTCCCCTGC AGCAGTTTAC CAGATGCTAG ACTCCGGACT TCCAAAGCTA 
TTGGCCCACG CCATTTACGA GTATTGCATG CATCTCATGC CGGGATCGGA GCCAGTAGCT 
TCTGCATCTA CAGTGCCGGA AGACGAACCG TTAGCCAACA ATCAGGCAGC CGCTGCAGCA 
GATGACGAGG ACTCGTCTCT TACGGACTTT GACAAGGCCG AGGCAAATGT GGTTTATAAT 
ACTCCACTGC TAAGTAGCTC GAATGTACCC AAAGTGCTAG ATTTCTTTGC CGAATGTTGT 
GCCGAAGGTC CAATGCGTGA TTGGTTAGGT ACTATGCAGG GCTCCGTATT TTGGAAGCCT 
CTGCTGCAGT TGCTCTGTAA CACACATGCC GCCCAGTTCA GCAAGACGTT GCCTAAGGAA 
CAATCGTTTA TCCGCCTGGA GAGGGCCACA ATCAACTTCT TTACGCGAGT TAGCGCCTGC 
CATCCCGGAA ACCAGGAAAA TCTTACATCG TTGCTCATTG GAGCCATAAT TTGCAAACCA 
TTAAGATTTT CGCATGGAGT TCGACCAACT ATATCTGGAT TTACTAGACA GATGGTGCTG 
CAGCTCCTCC TCGAGAATGA ACACATCACA GTCTCTGTTA GAAGTCAGCA GCCCTTGCAA 
CGGAGGGATA CCCTCTCCGC CGTTCTAGGC GGGATTTCGA GTGGTGCAAG TACTAGCTTA 
CCCGTAGCAG CAGTCAACAA TCATCCGGCC AGGAGGAGCA GCGCCCACCA CATGCTATTC 
ACGGTGACCA CTAGCACCAC GTGCCAAGAG ATTATGCAGA ATTGCGTTAG TGTGTTTAGT 
AATCTAGTTT ATCAACAACC AACAGATCAG CGAGCTGGCG AGACCTCTGC CTCGAGCAGC 
GGCGTATCGA GTGCAACTGC CAAATCCAGT GAGAGCAAAA TGGAAAAGAG CAGCTTCCTG 
AACTTTAACA ACGTAGAAGC GGGTAGCATG GAGTTCCTTA CCGTTGGTGC TGCTGTGGCA 
GCCAAGGACA AGCGCATAAA AGATGCTAAG AACCAAGCAG CCATGAACAA GGACAAGGAG 
ACTCAGACCA GCTCAACGAA CTTGGCTTCC AATATATTCA ATGTGGATGA GTTCTTGTTG 
CAATCGGCGA ATGCCGTTAA TGGTAACCAG TTGGTCATTC CAGAGTATTC TGATATTGTT 
CTAGCTGGAG ATGCCACCAT CTCGCAGGTT TTGGCCTCGC TTCAGAATGC AGGACATTCT 
CTTTCGACGC CCTGTATATC GTTTGATCTG GTACTTCAAC AGCGCTCTGA TGACACAAGC 
GAGGCAAAGA AGACCAAAGC TGCCTGCACT GAGCCGTTGC CATCACCACT GCAACGATTT 
TCCAGCAGTG GTGGTCTATC TTTACTCGCT CACTACCTGC CCACCGTATA TCCGGAACAC 
TCAGGTCGCA AGACCACCTT GGTGTTTAGC GAGAAGGAGA AAAGTCCCCC GCTCTCTGAC 
TGGGTGAAAT TGGAACCGAA CGACGAGATC TATGAGGATT TGGAGGACAC TCTCTGTGAG 
CCAGCAGCTA AGCTGGCAAC GGTCAGTTCG GTGCCGCAGC ACTCGCTGGC CGCATTTGGA 
TTATTCCTGC GCCTGCCCGC CTACTCTGAT GTCTTGCTGC GAGACAAGAT GCGGGCGCAG 
TGTCTTCTCC GCTTAGTGCT CGGAGTCACG GGAGATGGGG ATGGCAATGA TATATACAGT 
CTGTCGCTGG CACCCTCCTT GCCTACATTA CCCTTCGAGG TCTTCCGTCA GCTTCTTGAT 
AGTGTGCCGT TATCCACCGA CGACGGTGTT TTGCTACGAC GCGTGGTAAT CGAAGTGGGG 
GCCATGCACC TAGTGCTTAA TTGCCTCGGC ATTTTCTCGC ATCAGTCGCA CAACAACAGG 
ATTGGAGCAC CGATTAGCGA ACCATCGCCA AGTGGAACCA GTAGTGGTAG CGGCACCATT 
GGCAATGGAA GTGGAAACGG AAGTGTAAGC GGTACCAAAA CTAATGCACC AGAAGAGGCC 
ACACCTAGTG CCACGTCGGA CGACAAAAGC CACATGTACT GGGCCAAGGG CACTGGTTTC 
GGTACCGGTT CTACTACTCA GTCGTGGAAT GTGGAGCAGG CGCTTCTTCG GCAAAAGAGC 
GAGGAGGAGC ATGTCACAGT GTTGCTGCTT GTTCTAGCCA GTTACATAAA TCCGGGTGAC 
TGTATCCCAA GTGAAATGTG CGGAGAGGAC ATCCTAGCTT ATCATGATGT CCGAGATGTT 
ACCGGCGAGT TGCCGCCCAT CTTCCAAACC CTACTGCAGC AGTCTTGCTT AATCCCCGCC 
CTCAGCTCCT ATCTGCGCAA CGATTCAGTG CTTGATATCA CGCGCCACAT TCCGCTATAC 
CGTGCCATAC TCAAACTTCT GCGTGCCCTA TCGCTGTCTA AGATGCTTGT CTCGTTGCTC 
CAACCATCTG CCAGTGGCAG TGGGGACAGC ACGCCACCCA TAGTGGAGTT GCTCACAAGC 
ATGAAGACCT GTGTGGATAC CTACGCCAAA CGGTTAAAAG TTAACAAGAA GTCAAACATA 
AAGGGACAGA CACATCAGTT GACCTTTAAC ATCGATGATG GCGACGATGA GGGATTAGCT 
TTGCTTATTC CGGATATCCA TGAAACCAGT GTGCTGGTGC AAAAAACCAC AGATGCTGAT 
GCGCTAATCA ACATGTTGCA TACCAACGAA GACGGCAGCG CACAGCTAGA GCCCCAGAGC 
AAATCAATTG AACAGCGCTA CTTAGAGCTA ATGAAGAAGC GCCAGTTCGA TACGTTTGAC 
ATGATTGTGG AGTCGGATAA CAACAGTTTC CGCTTTGTGG TCTCGCATCA CTTCGAAAAA 
ATGGTTCGGC TCGCTGGCGA TCGTTACCAT CCGTCGCGGG TCAAGCGACT TGCCCAGGAG 
GCAGTTACAC TGTCCACCAG CCTACCCCTC AGCTTTAGTA GTTCTGTATT TGTACGCTGT 
GATACTGATA GATTGGACAT CATGAAGGTA CTCATAACTG GTCCGGCGGA CACGCCTTAC 
GCCAACGGGT GCTTTGAGTT TGATGTCTTC TTTCCGCCGG ACTACCCTAA CCAGCCCATG 
CTCATCAATC TGGAGACGAC GGGTCGCCAT TCGGTGCGCT TTAACCCAAA CCTCTATAAT 
GACGGCAAAG TTTGCCTGTC AGTTCTCAAC ACCTGGCATG GTCGGCCTGA GGAGAAATGG 
AATGCACAGA CTTCAAGCTT CCTGCAGGTA CTCGTTTCCA TCCAGTCCCT GATCCTCGTT 
CCCGAGCCCT ATTTCAATGA ACCGGGCTTT GAAAGAAGTC GTGGCAGTCC AAGTGGTACC 
AATTCATCGC GAGAATATAA TAGCAATATA TATCAGGCCT GTGTGCGGTG GGCTATGTTG 
GAGCAAATAC GGAGTCCAAG TCAGTGCTTT AAAGATGTCA TACACAAGCA CTTCTGGTTG 
AAGAGAGAGG AGATTTGCGC CCAGATAGAG GGCTGGATCG AGGAACTCGG TAAGCCGCAG 
TACACCGAGA GGGCCAGTCG CACTATATCG TTCAACTCAA TGGTCCTGCG CCGCCACTAT 
CGCCACCTGC GTGAGGAGTT GTCGAAACTG AAGCCGCCAC GTGGCCTCGA GGACTTGGAT 
GCGCCCTTTA ATCCTGTGGC CACGCTACCA CCGATGGATG TGTCCGCCGT GGCTCCTTCA 
CTAGCAGCCA CAAATACCGT CCAAGCGCAA CCGGATGATG CCATAGTGAC CGATATTACC 
CTGCTCGGCG AGAATGCGGA TGAATGCGAA GCTGATGGCG ATGCCGAAGG CGATGGAGAA 
GTGGACGATG GCTTTAAGGG TTTCCCGCAA GAAGAGGGTT TGCTGACGGG CGAGGAAGAG 
GTCGTGGAAA TTTTAAGTGA TACGGAGAAC GAGAGCAGTA TGTGGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649995.2
CDS	167..14797
Translation	MATEQHHQQRVLKQDGYLDLGDCNSAPSRPFEMRNVVYHPQLNVLLLFDNGSTVKVLDANSGVILQTYHLSSNGDSIVHGRYMPLQDKILFWDGHNLGLRGDYNGVLLLDTILQAPIGQNDDYIKLEIILSEAIIFQNCITELESEGLECPCDISNELTQKINQAQLKAKKGIKAQRWNTICLEVPYSSLKLVSNNMVILLKRLERHIPVLAIASAINERLTDMMMGSRVPDFGWNFSNFQRVLMHSEAVRRQTFEKWPHMDYKWALPDQMAQAGFYHQPSSSGEDRAMCFTCSVCLVCWEKTDEPWSEHERHSPLCPFVKGEYTQNVPLSITYATNPALPAPGLGFDIISNSDYANVLCTSCSQTGELSVWSIERHLKLMHTFHVPTLLNYIFEESFEWARVTAICVLPNARARTKVNYVYSAANYGGSGSSNGAGCNSGLNAVSGGKFGSVNAQHITSTSTLRAGVVGSKIVLGVSVRQSSGELALRMVVLNIVEVDRNSESSTSVSNDSGSVSSGGGQLMKSSSPMVNVSAENNATVTQVNLESKDDDNKAMTPYEKFADGFDSNGFVSALMTYNKNNGVSQNTDDASALYDFDLCTLSQILDHNLEEPLSLMVGSSNTVNVSNIGMGNEINKLVVDVEISHQNNNNNNNNNNEASSLGGNSSNNNNNIMINGGTNSSIDKMTAIEEIDCVVESWTSVKRMNALDGASSGVNGVNHMDQEVIEIAEILPTTPKCNQLLVKLLRTKAPEAEGSKIIMATVAKEEEEEDEEQRTDESMETDNNAEGNVAAQLLVFDYDDTEISNDYTLAMTFSRAKFPKQLCILPHFNSSPEHKDAGSICVVCADGTVEIYSLADFQGTMVIEEEGEHFESVVYCRNLDRLCCCSRQGGLLFYSLSDGENDSGDELLEMDDDCSTTLTQAADVSVTDGGRQLARTDLLGKTLLANESICMDAGSEAAAIPNSSSAAQGAHVQTSPSASSAPSTNANLLAYKTADLTLEDLKVFYALTQFDDKLTVYSAEVPSCWNDIGQVQNQRKQSQNMRHGGDERDFTRTWRLHNDATTWDEHIIELNLAQPVSLGHIDLKFTVYQQCSNPAAIQVTLLKQNNNGFGYRMKGPHSSYKPNVIDDNIDLSYIANENPVLSEEYLLAHNAEVLAGPIELSSCIDLCDQGGTATLTSPKLFKTRTRNFLLHIKTVSDPSKDGQNKTRGCDWLHEISISVRAVKSHSRISNGRMQRIAMLESNVLLVQLLRIASDPASSSLAKNLSLDILNWICFIRLNRFRSPKSERLKDQINKQTAANIQDLLAQQFECISLAEKHIEQLLRNCVIYSERSTAHKCVKLLIMLTEGATNLPAEVQDHTTLDYSFKAAIANTFPELPRAQCGSVVRWYMMLTCATSTAESHNSISEQAISLLKEIAAEIDKRWDPHCALLCTRFGLYGYPFEPEIFDFDFPNVSRQGPGSSVANVIRSSPTILPVTGLDIKRLSSIDGVDFRTFPYLIRGKSISNQLRGLLEVVQLHFNCVQTSEATRIDNIDSVGGNTGANVIIEDVMMMPKQVVAKEKGEEMLNDLVNDVECLVEEMKVDENEKQEKVDYDAFGLVNGFKDKTLSSLNSVKQTLKEEVKKEKADIMDKLTIAKFLPKLSLLESYMQKKINEHISFDDFGKLDNWDGNTTPPFNLEVEAPIVCSEATSESNMSQQATSAGGNILDDAADSEKITESLNANPCQPIAWHKLITPPPKQMIVIDRMHSGARRFVVLDFGQHILLTDLVIPSCDELTSLNIDIWCFDEETDCTRLVQVNDIQTKTLVLSDIQPAPICRYLKLTIIGRIGMSATKCKVPLGRFFGHPVVLEHDGYGDQLMKYIKHPTHSLQSQIKSLNALYEDVHCRYSLASSKLMDLLAPMRNSELSNVAHLQAFINKQREEELSGLENNNKVVSLYDECMLLQYQINVIRNVVLRLERALSPNSPTTTLLPGQSIPLTEDILRAASRDKLRVLSLSLVEVLLHFSIEYGIKNILPVHQRFNAATAKALFNALVVYGDAQLQLATCSLLVRMCCFQPWWGDFLADTFCSLFSAQNCKAFPQDRIFFLLTYLGRRSIAMGACRSIVIDAVLKTLVKLLAPISPHYKFQSEQPSTSANAADRNAAMGTTSDLQLITWLLLFLSVCLDDNERKDKSAARWDFMSCEGDFTKARPSATPNIKQLRCFKKRIIQQNKYLAQPYADWGKKIYMIQNEHPGIFMELSTKSKGAGPTKSTSTGGKINMGSSGLCVGGPSSSSGNTSKSAASSSSPKNQAVPGTDGERDSNFDKGLKSLRMDNIKVVIRGLFALLLEMDFDCNMDQFLLTCKVIARLVAACRPSVQLCKIVTIAQLQQLVRLSVWNDQQHPWAVHAITCLLQDLLDADKQFRDNDVTPTNDTPRTMEAAIQETRDLFSDYTSTASSCTEALFETLMPTAREFYQEAVKYNYLPSLIECDDTEFDEMLNDIDMIERTKPVTKKESNALSKNTFNFFCKSISIALDSRLDVGLELHVETQLRRLTNRTSLDLYSSLPQVLTNEFVSPPPEAAPWPEYLTQLWSGTEYNGDNTYVMFKRVFDSILAELHLEDTWVHLEQVLQMWLTLNCELSDKPYTSGITHSDVPKIPFGANAVQGLLLALAWHSDIKLRTWCLGFQCLFLACNSLPIGDDADCTRINEAIVNDENFEKVLLRFFSGYGMSSSIITNRCAGPTICKHLHELLLWLHSKSETSGIPCKRRLKDILLHVVLQLVQPGGSISNQQGPIDAQNQLVRDLLMMPNDKGDLNIALKITESVSFLVYNNISNGERLQCQRGNENSPVGNNFSNLFANVLGSENPNKQNAAICDNSLIINLLKLSSHMVLTEMPRQPSEVTIPEEEELSNQSQTDETKAEQLNTEGHRSKVPCIADWVLRHFPTMKRLFGTLSQCSTNTFTMMSSGCFFSPKSNMILDDPQTIADAVFSLMITLNDKASHPRLVVVPICRYLEETTIPRNATLPRLPHSEPFLWYISKVLEVPAAVQTFTQLGGIQIICHSLVRLNKTIINMQPGLISLIMQHLTRNTRFKRNNHVAASSACKKTTTGTGTTCPTSAQPPRSAAQYDGLINFAPFSHIVSENDATQSAEVLISNPNNSHRRPRSPAWTYMFYPNETHVDLTITLPTAILLKEVQLVPHTTSLASCPSAVALELSRDYGLGSIPVGAPMATTGLTCIRLKLAKAEVATSIVLRLYKPKDCGNVGLVQIAVLGQTIFGNRMHGLRSPSPFIDSLATCSSPVQTAVLAAMDADSMLEDDAFAKTSIGWVRILSRCFHVAALQPDSKLADLIAAYPAAYPGFLEACCSLLNIMPMIPSLAQQHLETILLKLSAYNHEISLQLLRTLLWHTTPQVFKLSSDSVCDLIYEVVTGSTEHLLDKLRVLIDWVMQLHDNYSKQARCNNPQSGFVKVIASILWKVHTQQQLPELPLLISSSLFETCFEWVSSLEHDEPLKSSFDSLLCALCFIKPELFNQLLVKLGVKLEPPARGQTDDSKVQSGSAWFRREGSENLSVLLQRPTFLKTLALACQSPAAVYQMLDSGLPKLLAHAIYEYCMHLMPGSEPVASASTVPEDEPLANNQAAAAADDEDSSLTDFDKAEANVVYNTPLLSSSNVPKVLDFFAECCAEGPMRDWLGTMQGSVFWKPLLQLLCNTHAAQFSKTLPKEQSFIRLERATINFFTRVSACHPGNQENLTSLLIGAIICKPLRFSHGVRPTISGFTRQMVLQLLLENEHITVSVRSQQPLQRRDTLSAVLGGISSGASTSLPVAAVNNHPARRSSAHHMLFTVTTSTTCQEIMQNCVSVFSNLVYQQPTDQRAGETSASSSGVSSATAKSSESKMEKSSFLNFNNVEAGSMEFLTVGAAVAAKDKRIKDAKNQAAMNKDKETQTSSTNLASNIFNVDEFLLQSANAVNGNQLVIPEYSDIVLAGDATISQVLASLQNAGHSLSTPCISFDLVLQQRSDDTSEAKKTKAACTEPLPSPLQRFSSSGGLSLLAHYLPTVYPEHSGRKTTLVFSEKEKSPPLSDWVKLEPNDEIYEDLEDTLCEPAAKLATVSSVPQHSLAAFGLFLRLPAYSDVLLRDKMRAQCLLRLVLGVTGDGDGNDIYSLSLAPSLPTLPFEVFRQLLDSVPLSTDDGVLLRRVVIEVGAMHLVLNCLGIFSHQSHNNRIGAPISEPSPSGTSSGSGTIGNGSGNGSVSGTKTNAPEEATPSATSDDKSHMYWAKGTGFGTGSTTQSWNVEQALLRQKSEEEHVTVLLLVLASYINPGDCIPSEMCGEDILAYHDVRDVTGELPPIFQTLLQQSCLIPALSSYLRNDSVLDITRHIPLYRAILKLLRALSLSKMLVSLLQPSASGSGDSTPPIVELLTSMKTCVDTYAKRLKVNKKSNIKGQTHQLTFNIDDGDDEGLALLIPDIHETSVLVQKTTDADALINMLHTNEDGSAQLEPQSKSIEQRYLELMKKRQFDTFDMIVESDNNSFRFVVSHHFEKMVRLAGDRYHPSRVKRLAQEAVTLSTSLPLSFSSSVFVRCDTDRLDIMKVLITGPADTPYANGCFEFDVFFPPDYPNQPMLINLETTGRHSVRFNPNLYNDGKVCLSVLNTWHGRPEEKWNAQTSSFLQVLVSIQSLILVPEPYFNEPGFERSRGSPSGTNSSREYNSNIYQACVRWAMLEQIRSPSQCFKDVIHKHFWLKREEICAQIEGWIEELGKPQYTERASRTISFNSMVLRRHYRHLREELSKLKPPRGLEDLDAPFNPVATLPPMDVSAVAPSLAATNTVQAQPDDAIVTDITLLGENADECEADGDAEGDGEVDDGFKGFPQEEGLLTGEEEVVEILSDTENESSMWQ

Target ORF information:

RefSeq Version	NM_141738.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141738.5

ORF Insert Sequence:

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCACTGCTT CCTCGTGTAC TGAGGCACTA 
TTTGAGACGC TGATGCCGAC AGCAAGAGAA TTTTATCAAG AGGCCGTCAA ATATAATTAC 
CTACCTTCAT TAATTGAATG CGATGACACG GAATTCGATG AAATGCTAAA CGACATTGAC 
ATGATTGAAC GAACGAAGCC AGTTACCAAA AAGGAAAGTA ATGCCCTGTC TAAGAATACA 
TTCAATTTCT TCTGCAAGTC TATCTCGATT GCTCTGGATT CACGATTGGA CGTGGGTCTG 
GAATTGCACG TAGAAACTCA GCTGAGAAGG CTAACAAATC GCACATCCCT GGATCTCTAT 
TCATCGCTTC CTCAGGTGTT GACCAATGAG TTTGTCTCTC CACCACCTGA GGCTGCTCCT 
TGGCCAGAAT ATTTGACTCA ACTTTGGTCA GGCACGGAAT ACAACGGTGA CAATACTTAT 
GTGATGTTTA AAAGGGTCTT TGACTCCATC CTGGCGGAAT TGCACCTTGA AGACACGTGG 
GTGCACTTGG AGCAGGTGCT GCAGATGTGG CTGACGCTAA ATTGCGAGCT CTCGGACAAG 
CCATACACCA GTGGCATCAC TCACTCGGAT GTTCCAAAAA TTCCGTTTGG AGCAAATGCG 
GTGCAGGGAT TGCTCCTGGC TTTGGCTTGG CACAGTGATA TAAAACTGCG GACCTGGTGT 
CTTGGCTTTC AGTGCCTGTT TCTCGCTTGC AACTCATTGC CTATCGGAGA TGATGCGGAT 
TGTACGCGTA TCAACGAGGC TATCGTGAAT GATGAAAACT TTGAAAAAGT GCTGCTACGA 
TTCTTTTCCG GTTATGGCAT GAGCTCATCT ATCATCACCA ACCGATGCGC TGGACCTACT 
ATTTGCAAGC ACTTGCATGA GTTATTGCTC TGGCTGCACA GCAAAAGCGA GACGAGTGGC 
ATTCCGTGCA AGCGTCGACT TAAGGACATT TTGTTGCATG TTGTGCTTCA GTTGGTGCAG 
CCTGGTGGCT CTATTAGCAA CCAGCAGGGT CCAATTGATG CTCAAAACCA GCTGGTTCGT 
GATTTGCTTA TGATGCCCAA TGATAAGGGG GACCTCAACA TTGCGCTTAA AATCACAGAA 
AGTGTTTCTT TCCTGGTGTA CAACAACATA TCCAATGGCG AGAGGCTACA ATGTCAGCGT 
GGAAATGAAA ACAGCCCAGT TGGCAACAAT TTTAGCAATT TGTTTGCCAA CGTTCTGGGA 
TCAGAAAATC CCAACAAGCA GAATGCCGCA ATTTGCGACA ACTCACTTAT TATAAATCTG 
CTGAAACTAT CGTCACACAT GGTATTGACA GAGATGCCCC GGCAACCAAG TGAGGTTACC 
ATTCCCGAGG AGGAGGAACT TTCCAATCAG AGTCAAACGG ATGAGACTAA AGCGGAGCAG 
TTAAACACGG AAGGACATCG CAGCAAAGTG CCTTGCATTG CAGATTGGGT ACTCCGCCAT 
TTCCCCACAA TGAAGCGACT TTTTGGAACG CTTTCCCAGT GCTCGACAAA CACCTTCACC 
ATGATGTCCT CTGGCTGTTT CTTTTCACCG AAGTCCAATA TGATTTTAGA TGACCCGCAG 
ACAATTGCAG ACGCCGTCTT TAGTTTGATG ATTACTCTCA ACGACAAGGC TTCGCATCCC 
CGACTTGTTG TGGTGCCCAT TTGCCGATAC CTTGAAGAAA CCACTATTCC GAGAAATGCA 
ACGCTGCCGC GTCTGCCGCA TTCTGAACCT TTCCTGTGGT ACATCTCGAA GGTATTGGAA 
GTACCAGCGG CAGTGCAAAC GTTCACCCAA TTGGGCGGAA TTCAGATTAT ATGTCATAGC 
CTGGTGCGAC TTAATAAAAC TATCATCAAC ATGCAACCAG GATTGATTTC TTTGATTATG 
CAACACTTGA CGCGCAATAC GCGCTTTAAG CGTAATAATC ATGTGGCGGC AAGTAGTGCT 
TGCAAAAAGA CAACGACTGG TACGGGAACC ACATGTCCCA CGTCAGCACA GCCGCCGAGA 
AGTGCTGCTC AGTACGATGG TCTGATCAAC TTTGCACCCT TCTCGCATAT TGTCTCCGAA 
AATGATGCGA CACAAAGTGC AGAGGTACTA ATATCAAATC CAAATAATTC ACATCGAAGA 
CCGCGATCGC CAGCCTGGAC CTATATGTTC TATCCAAATG AGACGCATGT GGATCTGACT 
ATAACGTTGC CCACAGCAAT TTTGTTGAAA GAGGTGCAGC TGGTGCCGCA CACAACTAGT 
TTGGCATCCT GTCCATCTGC GGTGGCTCTA GAGCTGTCTA GAGATTACGG CCTTGGTTCG 
ATCCCAGTGG GTGCTCCCAT GGCCACCACT GGTCTGACCT GCATTAGACT AAAGCTGGCC 
AAGGCAGAGG TGGCTACCAG CATTGTTCTG AGGCTGTACA AACCCAAGGA CTGTGGAAAT 
GTGGGTCTCG TACAAATCGC TGTGCTTGGT CAGACAATCT TCGGAAATCG CATGCATGGC 
CTTCGCTCAC CGAGTCCGTT CATTGACTCC CTAGCAACGT GCTCCTCGCC AGTGCAAACG 
GCAGTTTTAG CCGCAATGGA TGCGGATTCC ATGCTTGAAG ATGATGCTTT TGCGAAGACT 
AGCATTGGCT GGGTTCGCAT TCTGTCGCGC TGTTTCCATG TGGCCGCTTT ACAACCAGAC 
AGTAAGCTGG CTGATCTTAT CGCCGCCTAT CCGGCTGCAT ATCCCGGCTT TTTAGAGGCC 
TGCTGCTCAT TGCTAAACAT AATGCCAATG ATTCCTAGTC TTGCACAGCA ACACTTGGAG 
ACCATTTTGT TAAAATTAAG TGCATACAAC CACGAGATCA GTTTGCAACT TCTTAGGACG 
CTCCTGTGGC ACACCACTCC GCAAGTTTTT AAACTCTCCA GCGATTCGGT GTGTGATCTT 
ATTTATGAAG TAGTTACCGG ATCGACGGAG CACCTACTGG ACAAACTACG CGTGCTTATC 
GACTGGGTAA TGCAATTGCA CGACAACTAC AGCAAGCAGG CGCGCTGTAA TAACCCACAA 
AGTGGATTTG TTAAGGTGAT TGCTTCGATT CTGTGGAAAG TGCATACCCA GCAACAATTG 
CCTGAGTTAC CGCTTCTCAT TTCATCTTCG CTGTTCGAGA CCTGTTTTGA ATGGGTCTCT 
TCGCTGGAGC ACGATGAGCC ACTGAAGAGC AGTTTTGACT CCTTGTTGTG TGCATTGTGC 
TTCATTAAGC CAGAACTGTT TAACCAGCTG TTGGTCAAGC TGGGAGTTAA GCTGGAACCT 
CCAGCTCGAG GTCAAACTGA TGACAGCAAA GTGCAAAGTG GTAGTGCTTG GTTCCGCCGT 
GAGGGTAGCG AAAACCTTAG TGTTTTGCTA CAGCGTCCCA CATTCCTAAA GACTCTAGCC 
TTGGCCTGCC AGTCCCCTGC AGCAGTTTAC CAGATGCTAG ACTCCGGACT TCCAAAGCTA 
TTGGCCCACG CCATTTACGA GTATTGCATG CATCTCATGC CGGGATCGGA GCCAGTAGCT 
TCTGCATCTA CAGTGCCGGA AGACGAACCG TTAGCCAACA ATCAGGCAGC CGCTGCAGCA 
GATGACGAGG ACTCGTCTCT TACGGACTTT GACAAGGCCG AGGCAAATGT GGTTTATAAT 
ACTCCACTGC TAAGTAGCTC GAATGTACCC AAAGTGCTAG ATTTCTTTGC CGAATGTTGT 
GCCGAAGGTC CAATGCGTGA TTGGTTAGGT ACTATGCAGG GCTCCGTATT TTGGAAGCCT 
CTGCTGCAGT TGCTCTGTAA CACACATGCC GCCCAGTTCA GCAAGACGTT GCCTAAGGAA 
CAATCGTTTA TCCGCCTGGA GAGGGCCACA ATCAACTTCT TTACGCGAGT TAGCGCCTGC 
CATCCCGGAA ACCAGGAAAA TCTTACATCG TTGCTCATTG GAGCCATAAT TTGCAAACCA 
TTAAGATTTT CGCATGGAGT TCGACCAACT ATATCTGGAT TTACTAGACA GATGGTGCTG 
CAGCTCCTCC TCGAGAATGA ACACATCACA GTCTCTGTTA GAAGTCAGCA GCCCTTGCAA 
CGGAGGGATA CCCTCTCCGC CGTTCTAGGC GGGATTTCGA GTGGTGCAAG TACTAGCTTA 
CCCGTAGCAG CAGTCAACAA TCATCCGGCC AGGAGGAGCA GCGCCCACCA CATGCTATTC 
ACGGTGACCA CTAGCACCAC GTGCCAAGAG ATTATGCAGA ATTGCGTTAG TGTGTTTAGT 
AATCTAGTTT ATCAACAACC AACAGATCAG CGAGCTGGCG AGACCTCTGC CTCGAGCAGC 
GGCGTATCGA GTGCAACTGC CAAATCCAGT GAGAGCAAAA TGGAAAAGAG CAGCTTCCTG 
AACTTTAACA ACGTAGAAGC GGGTAGCATG GAGTTCCTTA CCGTTGGTGC TGCTGTGGCA 
GCCAAGGACA AGCGCATAAA AGATGCTAAG AACCAAGCAG CCATGAACAA GGACAAGGAG 
ACTCAGACCA GCTCAACGAA CTTGGCTTCC AATATATTCA ATGTGGATGA GTTCTTGTTG 
CAATCGGCGA ATGCCGTTAA TGGTAACCAG TTGGTCATTC CAGAGTATTC TGATATTGTT 
CTAGCTGGAG ATGCCACCAT CTCGCAGGTT TTGGCCTCGC TTCAGAATGC AGGACATTCT 
CTTTCGACGC CCTGTATATC GTTTGATCTG GTACTTCAAC AGCGCTCTGA TGACACAAGC 
GAGGCAAAGA AGACCAAAGC TGCCTGCACT GAGCCGTTGC CATCACCACT GCAACGATTT 
TCCAGCAGTG GTGGTCTATC TTTACTCGCT CACTACCTGC CCACCGTATA TCCGGAACAC 
TCAGGTCGCA AGACCACCTT GGTGTTTAGC GAGAAGGAGA AAAGTCCCCC GCTCTCTGAC 
TGGGTGAAAT TGGAACCGAA CGACGAGATC TATGAGGATT TGGAGGACAC TCTCTGTGAG 
CCAGCAGCTA AGCTGGCAAC GGTCAGTTCG GTGCCGCAGC ACTCGCTGGC CGCATTTGGA 
TTATTCCTGC GCCTGCCCGC CTACTCTGAT GTCTTGCTGC GAGACAAGAT GCGGGCGCAG 
TGTCTTCTCC GCTTAGTGCT CGGAGTCACG GGAGATGGGG ATGGCAATGA TATATACAGT 
CTGTCGCTGG CACCCTCCTT GCCTACATTA CCCTTCGAGG TCTTCCGTCA GCTTCTTGAT 
AGTGTGCCGT TATCCACCGA CGACGGTGTT TTGCTACGAC GCGTGGTAAT CGAAGTGGGG 
GCCATGCACC TAGTGCTTAA TTGCCTCGGC ATTTTCTCGC ATCAGTCGCA CAACAACAGG 
ATTGGAGCAC CGATTAGCGA ACCATCGCCA AGTGGAACCA GTAGTGGTAG CGGCACCATT 
GGCAATGGAA GTGGAAACGG AAGTGTAAGC GGTACCAAAA CTAATGCACC AGAAGAGGCC 
ACACCTAGTG CCACGTCGGA CGACAAAAGC CACATGTACT GGGCCAAGGG CACTGGTTTC 
GGTACCGGTT CTACTACTCA GTCGTGGAAT GTGGAGCAGG CGCTTCTTCG GCAAAAGAGC 
GAGGAGGAGC ATGTCACAGT GTTGCTGCTT GTTCTAGCCA GTTACATAAA TCCGGGTGAC 
TGTATCCCAA GTGAAATGTG CGGAGAGGAC ATCCTAGCTT ATCATGATGT CCGAGATGTT 
ACCGGCGAGT TGCCGCCCAT CTTCCAAACC CTACTGCAGC AGTCTTGCTT AATCCCCGCC 
CTCAGCTCCT ATCTGCGCAA CGATTCAGTG CTTGATATCA CGCGCCACAT TCCGCTATAC 
CGTGCCATAC TCAAACTTCT GCGTGCCCTA TCGCTGTCTA AGATGCTTGT CTCGTTGCTC 
CAACCATCTG CCAGTGGCAG TGGGGACAGC ACGCCACCCA TAGTGGAGTT GCTCACAAGC 
ATGAAGACCT GTGTGGATAC CTACGCCAAA CGGTTAAAAG TTAACAAGAA GTCAAACATA 
AAGGGACAGA CACATCAGTT GACCTTTAAC ATCGATGATG GCGACGATGA GGGATTAGCT 
TTGCTTATTC CGGATATCCA TGAAACCAGT GTGCTGGTGC AAAAAACCAC AGATGCTGAT 
GCGCTAATCA ACATGTTGCA TACCAACGAA GACGGCAGCG CACAGCTAGA GCCCCAGAGC 
AAATCAATTG AACAGCGCTA CTTAGAGCTA ATGAAGAAGC GCCAGTTCGA TACGTTTGAC 
ATGATTGTGG AGTCGGATAA CAACAGTTTC CGCTTTGTGG TCTCGCATCA CTTCGAAAAA 
ATGGTTCGGC TCGCTGGCGA TCGTTACCAT CCGTCGCGGG TCAAGCGACT TGCCCAGGAG 
GCAGTTACAC TGTCCACCAG CCTACCCCTC AGCTTTAGTA GTTCTGTATT TGTACGCTGT 
GATACTGATA GATTGGACAT CATGAAGGTA CTCATAACTG GTCCGGCGGA CACGCCTTAC 
GCCAACGGGT GCTTTGAGTT TGATGTCTTC TTTCCGCCGG ACTACCCTAA CCAGCCCATG 
CTCATCAATC TGGAGACGAC GGGTCGCCAT TCGGTGCGCT TTAACCCAAA CCTCTATAAT 
GACGGCAAAG TTTGCCTGTC AGTTCTCAAC ACCTGGCATG GTCGGCCTGA GGAGAAATGG 
AATGCACAGA CTTCAAGCTT CCTGCAGGTA CTCGTTTCCA TCCAGTCCCT GATCCTCGTT 
CCCGAGCCCT ATTTCAATGA ACCGGGCTTT GAAAGAAGTC GTGGCAGTCC AAGTGGTACC 
AATTCATCGC GAGAATATAA TAGCAATATA TATCAGGCCT GTGTGCGGTG GGCTATGTTG 
GAGCAAATAC GGAGTCCAAG TCAGTGCTTT AAAGATGTCA TACACAAGCA CTTCTGGTTG 
AAGAGAGAGG AGATTTGCGC CCAGATAGAG GGCTGGATCG AGGAACTCGG TAAGCCGCAG 
TACACCGAGA GGGCCAGTCG CACTATATCG TTCAACTCAA TGGTCCTGCG CCGCCACTAT 
CGCCACCTGC GTGAGGAGTT GTCGAAACTG AAGCCGCCAC GTGGCCTCGA GGACTTGGAT 
GCGCCCTTTA ATCCTGTGGC CACGCTACCA CCGATGGATG TGTCCGCCGT GGCTCCTTCA 
CTAGCAGCCA CAAATACCGT CCAAGCGCAA CCGGATGATG CCATAGTGAC CGATATTACC 
CTGCTCGGCG AGAATGCGGA TGAATGCGAA GCTGATGGCG ATGCCGAAGG CGATGGAGAA 
GTGGACGATG GCTTTAAGGG TTTCCCGCAA GAAGAGGGTT TGCTGACGGG CGAGGAAGAG 
GTCGTGGAAA TTTTAAGTGA TACGGAGAAC GAGAGCAGTA TGTGGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bruce ( NM_001275533.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262462.1 Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant D, mRNA.

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CloneID	OFa08255
Clone ID Related Accession (Same CDS sequence)	NM_001275533.2
Accession Version	NM_001275533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BIR repeat containing ubiquitin-conjugating enzyme, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275533.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCTCGCT GGGACTTCAT GAGCTGCGAG 
GGCGATTTTA CTAAGGCCAG ACCTAGTGCA ACTCCCAACA TCAAACAGCT ACGATGCTTT 
AAAAAACGCA TCATTCAGCA AAACAAGTAC TTGGCGCAGC CTTACGCTGA CTGGGGAAAG 
AAGATTTATA TGATACAGAA TGAGCATCCA GGCATTTTCA TGGAGCTGTC AACGAAATCA 
AAGGGCGCTG GACCAACCAA GTCGACCTCT ACAGGTGGCA AGATTAATAT GGGATCCTCT 
GGCCTGTGCG TGGGTGGTCC TTCAAGTTCC AGTGGCAATA CATCAAAGAG TGCGGCTTCC 
TCATCCTCTC CTAAGAATCA GGCTGTGCCG GGTACCGATG GCGAACGAGA CAGCAACTTT 
GACAAGGGAT TAAAATCACT TCGAATGGAT AACATTAAGG TGGTTATTCG CGGTCTCTTT 
GCACTGTTGC TTGAAATGGA CTTCGATTGC AATATGGACC AGTTTTTGCT CACTTGCAAA 
GTCATTGCCC GTTTGGTGGC TGCATGCAGA CCGTCGGTAC AGCTGTGTAA GATTGTGACC 
ATCGCCCAAC TGCAGCAACT TGTACGTCTA TCCGTGTGGA ATGATCAGCA GCATCCCTGG 
GCTGTTCATG CCATTACGTG TTTACTGCAA GATCTTTTGG ATGCGGACAA GCAGTTCCGG 
GACAATGATG TCACACCTAC AAATGACACG CCGCGTACAA TGGAAGCTGC GATCCAGGAA 
ACACGGGATC TTTTCAGCGA CTATACTTCC ACTGCTTCCT CGTGTACTGA GGCACTATTT 
GAGACGCTGA TGCCGACAGC AAGAGAATTT TATCAAGAGG CCGTCAAATA TAATTACCTA 
CCTTCATTAA TTGAATGCGA TGACACGGAA TTCGATGAAA TGCTAAACGA CATTGACATG 
ATTGAACGAA CGAAGCCAGT TACCAAAAAG GAAAGTAATG CCCTGTCTAA GAATACATTC 
AATTTCTTCT GCAAGTCTAT CTCGATTGCT CTGGATTCAC GATTGGACGT GGGTCTGGAA 
TTGCACGTAG AAACTCAGCT GAGAAGGCTA ACAAATCGCA CATCCCTGGA TCTCTATTCA 
TCGCTTCCTC AGGTGTTGAC CAATGAGTTT GTCTCTCCAC CACCTGAGGC TGCTCCTTGG 
CCAGAATATT TGACTCAACT TTGGTCAGGC ACGGAATACA ACGGTGACAA TACTTATGTG 
ATGTTTAAAA GGGTCTTTGA CTCCATCCTG GCGGAATTGC ACCTTGAAGA CACGTGGGTG 
CACTTGGAGC AGGTGCTGCA GATGTGGCTG ACGCTAAATT GCGAGCTCTC GGACAAGCCA 
TACACCAGTG GCATCACTCA CTCGGATGTT CCAAAAATTC CGTTTGGAGC AAATGCGGTG 
CAGGGATTGC TCCTGGCTTT GGCTTGGCAC AGTGATATAA AACTGCGGAC CTGGTGTCTT 
GGCTTTCAGT GCCTGTTTCT CGCTTGCAAC TCATTGCCTA TCGGAGATGA TGCGGATTGT 
ACGCGTATCA ACGAGGCTAT CGTGAATGAT GAAAACTTTG AAAAAGTGCT GCTACGATTC 
TTTTCCGGTT ATGGCATGAG CTCATCTATC ATCACCAACC GATGCGCTGG ACCTACTATT 
TGCAAGCACT TGCATGAGTT ATTGCTCTGG CTGCACAGCA AAAGCGAGAC GAGTGGCATT 
CCGTGCAAGC GTCGACTTAA GGACATTTTG TTGCATGTTG TGCTTCAGTT GGTGCAGCCT 
GGTGGCTCTA TTAGCAACCA GCAGGGTCCA ATTGATGCTC AAAACCAGCT GGTTCGTGAT 
TTGCTTATGA TGCCCAATGA TAAGGGGGAC CTCAACATTG CGCTTAAAAT CACAGAAAGT 
GTTTCTTTCC TGGTGTACAA CAACATATCC AATGGCGAGA GGCTACAATG TCAGCGTGGA 
AATGAAAACA GCCCAGTTGG CAACAATTTT AGCAATTTGT TTGCCAACGT TCTGGGATCA 
GAAAATCCCA ACAAGCAGAA TGCCGCAATT TGCGACAACT CACTTATTAT AAATCTGCTG 
AAACTATCGT CACACATGGT ATTGACAGAG ATGCCCCGGC AACCAAGTGA GGTTACCATT 
CCCGAGGAGG AGGAACTTTC CAATCAGAGT CAAACGGATG AGACTAAAGC GGAGCAGTTA 
AACACGGAAG GACATCGCAG CAAAGTGCCT TGCATTGCAG ATTGGGTACT CCGCCATTTC 
CCCACAATGA AGCGACTTTT TGGAACGCTT TCCCAGTGCT CGACAAACAC CTTCACCATG 
ATGTCCTCTG GCTGTTTCTT TTCACCGAAG TCCAATATGA TTTTAGATGA CCCGCAGACA 
ATTGCAGACG CCGTCTTTAG TTTGATGATT ACTCTCAACG ACAAGGCTTC GCATCCCCGA 
CTTGTTGTGG TGCCCATTTG CCGATACCTT GAAGAAACCA CTATTCCGAG AAATGCAACG 
CTGCCGCGTC TGCCGCATTC TGAACCTTTC CTGTGGTACA TCTCGAAGGT ATTGGAAGTA 
CCAGCGGCAG TGCAAACGTT CACCCAATTG GGCGGAATTC AGATTATATG TCATAGCCTG 
GTGCGACTTA ATAAAACTAT CATCAACATG CAACCAGGAT TGATTTCTTT GATTATGCAA 
CACTTGACGC GCAATACGCG CTTTAAGCGT AATAATCATG TGGCGGCAAG TAGTGCTTGC 
AAAAAGACAA CGACTGGTAC GGGAACCACA TGTCCCACGT CAGCACAGCC GCCGAGAAGT 
GCTGCTCAGT ACGATGGTCT GATCAACTTT GCACCCTTCT CGCATATTGT CTCCGAAAAT 
GATGCGACAC AAAGTGCAGA GGTACTAATA TCAAATCCAA ATAATTCACA TCGAAGACCG 
CGATCGCCAG CCTGGACCTA TATGTTCTAT CCAAATGAGA CGCATGTGGA TCTGACTATA 
ACGTTGCCCA CAGCAATTTT GTTGAAAGAG GTGCAGCTGG TGCCGCACAC AACTAGTTTG 
GCATCCTGTC CATCTGCGGT GGCTCTAGAG CTGTCTAGAG ATTACGGCCT TGGTTCGATC 
CCAGTGGGTG CTCCCATGGC CACCACTGGT CTGACCTGCA TTAGACTAAA GCTGGCCAAG 
GCAGAGGTGG CTACCAGCAT TGTTCTGAGG CTGTACAAAC CCAAGGACTG TGGAAATGTG 
GGTCTCGTAC AAATCGCTGT GCTTGGTCAG ACAATCTTCG GAAATCGCAT GCATGGCCTT 
CGCTCACCGA GTCCGTTCAT TGACTCCCTA GCAACGTGCT CCTCGCCAGT GCAAACGGCA 
GTTTTAGCCG CAATGGATGC GGATTCCATG CTTGAAGATG ATGCTTTTGC GAAGACTAGC 
ATTGGCTGGG TTCGCATTCT GTCGCGCTGT TTCCATGTGG CCGCTTTACA ACCAGACAGT 
AAGCTGGCTG ATCTTATCGC CGCCTATCCG GCTGCATATC CCGGCTTTTT AGAGGCCTGC 
TGCTCATTGC TAAACATAAT GCCAATGATT CCTAGTCTTG CACAGCAACA CTTGGAGACC 
ATTTTGTTAA AATTAAGTGC ATACAACCAC GAGATCAGTT TGCAACTTCT TAGGACGCTC 
CTGTGGCACA CCACTCCGCA AGTTTTTAAA CTCTCCAGCG ATTCGGTGTG TGATCTTATT 
TATGAAGTAG TTACCGGATC GACGGAGCAC CTACTGGACA AACTACGCGT GCTTATCGAC 
TGGGTAATGC AATTGCACGA CAACTACAGC AAGCAGGCGC GCTGTAATAA CCCACAAAGT 
GGATTTGTTA AGGTGATTGC TTCGATTCTG TGGAAAGTGC ATACCCAGCA ACAATTGCCT 
GAGTTACCGC TTCTCATTTC ATCTTCGCTG TTCGAGACCT GTTTTGAATG GGTCTCTTCG 
CTGGAGCACG ATGAGCCACT GAAGAGCAGT TTTGACTCCT TGTTGTGTGC ATTGTGCTTC 
ATTAAGCCAG AACTGTTTAA CCAGCTGTTG GTCAAGCTGG GAGTTAAGCT GGAACCTCCA 
GCTCGAGGTC AAACTGATGA CAGCAAAGTG CAAAGTGGTA GTGCTTGGTT CCGCCGTGAG 
GGTAGCGAAA ACCTTAGTGT TTTGCTACAG CGTCCCACAT TCCTAAAGAC TCTAGCCTTG 
GCCTGCCAGT CCCCTGCAGC AGTTTACCAG ATGCTAGACT CCGGACTTCC AAAGCTATTG 
GCCCACGCCA TTTACGAGTA TTGCATGCAT CTCATGCCGG GATCGGAGCC AGTAGCTTCT 
GCATCTACAG TGCCGGAAGA CGAACCGTTA GCCAACAATC AGGCAGCCGC TGCAGCAGAT 
GACGAGGACT CGTCTCTTAC GGACTTTGAC AAGGCCGAGG CAAATGTGGT TTATAATACT 
CCACTGCTAA GTAGCTCGAA TGTACCCAAA GTGCTAGATT TCTTTGCCGA ATGTTGTGCC 
GAAGGTCCAA TGCGTGATTG GTTAGGTACT ATGCAGGGCT CCGTATTTTG GAAGCCTCTG 
CTGCAGTTGC TCTGTAACAC ACATGCCGCC CAGTTCAGCA AGACGTTGCC TAAGGAACAA 
TCGTTTATCC GCCTGGAGAG GGCCACAATC AACTTCTTTA CGCGAGTTAG CGCCTGCCAT 
CCCGGAAACC AGGAAAATCT TACATCGTTG CTCATTGGAG CCATAATTTG CAAACCATTA 
AGATTTTCGC ATGGAGTTCG ACCAACTATA TCTGGATTTA CTAGACAGAT GGTGCTGCAG 
CTCCTCCTCG AGAATGAACA CATCACAGTC TCTGTTAGAA GTCAGCAGCC CTTGCAACGG 
AGGGATACCC TCTCCGCCGT TCTAGGCGGG ATTTCGAGTG GTGCAAGTAC TAGCTTACCC 
GTAGCAGCAG TCAACAATCA TCCGGCCAGG AGGAGCAGCG CCCACCACAT GCTATTCACG 
GTGACCACTA GCACCACGTG CCAAGAGATT ATGCAGAATT GCGTTAGTGT GTTTAGTAAT 
CTAGTTTATC AACAACCAAC AGATCAGCGA GCTGGCGAGA CCTCTGCCTC GAGCAGCGGC 
GTATCGAGTG CAACTGCCAA ATCCAGTGAG AGCAAAATGG AAAAGAGCAG CTTCCTGAAC 
TTTAACAACG TAGAAGCGGG TAGCATGGAG TTCCTTACCG TTGGTGCTGC TGTGGCAGCC 
AAGGACAAGC GCATAAAAGA TGCTAAGAAC CAAGCAGCCA TGAACAAGGA CAAGGAGACT 
CAGACCAGCT CAACGAACTT GGCTTCCAAT ATATTCAATG TGGATGAGTT CTTGTTGCAA 
TCGGCGAATG CCGTTAATGG TAACCAGTTG GTCATTCCAG AGTATTCTGA TATTGTTCTA 
GCTGGAGATG CCACCATCTC GCAGGTTTTG GCCTCGCTTC AGAATGCAGG ACATTCTCTT 
TCGACGCCCT GTATATCGTT TGATCTGGTA CTTCAACAGC GCTCTGATGA CACAAGCGAG 
GCAAAGAAGA CCAAAGCTGC CTGCACTGAG CCGTTGCCAT CACCACTGCA ACGATTTTCC 
AGCAGTGGTG GTCTATCTTT ACTCGCTCAC TACCTGCCCA CCGTATATCC GGAACACTCA 
GGTCGCAAGA CCACCTTGGT GTTTAGCGAG AAGGAGAAAA GTCCCCCGCT CTCTGACTGG 
GTGAAATTGG AACCGAACGA CGAGATCTAT GAGGATTTGG AGGACACTCT CTGTGAGCCA 
GCAGCTAAGC TGGCAACGGT CAGTTCGGTG CCGCAGCACT CGCTGGCCGC ATTTGGATTA 
TTCCTGCGCC TGCCCGCCTA CTCTGATGTC TTGCTGCGAG ACAAGATGCG GGCGCAGTGT 
CTTCTCCGCT TAGTGCTCGG AGTCACGGGA GATGGGGATG GCAATGATAT ATACAGTCTG 
TCGCTGGCAC CCTCCTTGCC TACATTACCC TTCGAGGTCT TCCGTCAGCT TCTTGATAGT 
GTGCCGTTAT CCACCGACGA CGGTGTTTTG CTACGACGCG TGGTAATCGA AGTGGGGGCC 
ATGCACCTAG TGCTTAATTG CCTCGGCATT TTCTCGCATC AGTCGCACAA CAACAGGATT 
GGAGCACCGA TTAGCGAACC ATCGCCAAGT GGAACCAGTA GTGGTAGCGG CACCATTGGC 
AATGGAAGTG GAAACGGAAG TGTAAGCGGT ACCAAAACTA ATGCACCAGA AGAGGCCACA 
CCTAGTGCCA CGTCGGACGA CAAAAGCCAC ATGTACTGGG CCAAGGGCAC TGGTTTCGGT 
ACCGGTTCTA CTACTCAGTC GTGGAATGTG GAGCAGGCGC TTCTTCGGCA AAAGAGCGAG 
GAGGAGCATG TCACAGTGTT GCTGCTTGTT CTAGCCAGTT ACATAAATCC GGGTGACTGT 
ATCCCAAGTG AAATGTGCGG AGAGGACATC CTAGCTTATC ATGATGTCCG AGATGTTACC 
GGCGAGTTGC CGCCCATCTT CCAAACCCTA CTGCAGCAGT CTTGCTTAAT CCCCGCCCTC 
AGCTCCTATC TGCGCAACGA TTCAGTGCTT GATATCACGC GCCACATTCC GCTATACCGT 
GCCATACTCA AACTTCTGCG TGCCCTATCG CTGTCTAAGA TGCTTGTCTC GTTGCTCCAA 
CCATCTGCCA GTGGCAGTGG GGACAGCACG CCACCCATAG TGGAGTTGCT CACAAGCATG 
AAGACCTGTG TGGATACCTA CGCCAAACGG TTAAAAGTTA ACAAGAAGTC AAACATAAAG 
GGACAGACAC ATCAGTTGAC CTTTAACATC GATGATGGCG ACGATGAGGG ATTAGCTTTG 
CTTATTCCGG ATATCCATGA AACCAGTGTG CTGGTGCAAA AAACCACAGA TGCTGATGCG 
CTAATCAACA TGTTGCATAC CAACGAAGAC GGCAGCGCAC AGCTAGAGCC CCAGAGCAAA 
TCAATTGAAC AGCGCTACTT AGAGCTAATG AAGAAGCGCC AGTTCGATAC GTTTGACATG 
ATTGTGGAGT CGGATAACAA CAGTTTCCGC TTTGTGGTCT CGCATCACTT CGAAAAAATG 
GTTCGGCTCG CTGGCGATCG TTACCATCCG TCGCGGGTCA AGCGACTTGC CCAGGAGGCA 
GTTACACTGT CCACCAGCCT ACCCCTCAGC TTTAGTAGTT CTGTATTTGT ACGCTGTGAT 
ACTGATAGAT TGGACATCAT GAAGGTACTC ATAACTGGTC CGGCGGACAC GCCTTACGCC 
AACGGGTGCT TTGAGTTTGA TGTCTTCTTT CCGCCGGACT ACCCTAACCA GCCCATGCTC 
ATCAATCTGG AGACGACGGG TCGCCATTCG GTGCGCTTTA ACCCAAACCT CTATAATGAC 
GGCAAAGTTT GCCTGTCAGT TCTCAACACC TGGCATGGTC GGCCTGAGGA GAAATGGAAT 
GCACAGACTT CAAGCTTCCT GCAGGTACTC GTTTCCATCC AGTCCCTGAT CCTCGTTCCC 
GAGCCCTATT TCAATGAACC GGGCTTTGAA AGAAGTCGTG GCAGTCCAAG TGGTACCAAT 
TCATCGCGAG AATATAATAG CAATATATAT CAGGCCTGTG TGCGGTGGGC TATGTTGGAG 
CAAATACGGA GTCCAAGTCA GTGCTTTAAA GATGTCATAC ACAAGCACTT CTGGTTGAAG 
AGAGAGGAGA TTTGCGCCCA GATAGAGGGC TGGATCGAGG AACTCGGTAA GCCGCAGTAC 
ACCGAGAGGG CCAGTCGCAC TATATCGTTC AACTCAATGG TCCTGCGCCG CCACTATCGC 
CACCTGCGTG AGGAGTTGTC GAAACTGAAG CCGCCACGTG GCCTCGAGGA CTTGGATGCG 
CCCTTTAATC CTGTGGCCAC GCTACCACCG ATGGATGTGT CCGCCGTGGC TCCTTCACTA 
GCAGCCACAA ATACCGTCCA AGCGCAACCG GATGATGCCA TAGTGACCGA TATTACCCTG 
CTCGGCGAGA ATGCGGATGA ATGCGAAGCT GATGGCGATG CCGAAGGCGA TGGAGAAGTG 
GACGATGGCT TTAAGGGTTT CCCGCAAGAA GAGGGTTTGC TGACGGGCGA GGAAGAGGTC 
GTGGAAATTT TAAGTGATAC GGAGAACGAG AGCAGTATGT GGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262462.1
CDS	167..14794
Translation	MATEQHHQQRVLKQDGYLDLGDCNSAPSRPFEMRNVVYHPQLNVLLLFDNGSTVKVLDANSGVILQTYHLSSNGDSIVHGRYMPLQDKILFWDGHNLGLRGDYNGVLLLDTILQAPIGQNDDYIKLEIILSEAIIFQNCITELESEGLECPCDISNELTQKINQAQLKAKKGIKAQRWNTICLEVPYSSLKLVSNNMVILLKRLERHIPVLAIASAINERLTDMMMGSRVPDFGWNFSNFQRVLMHSEAVRRQTFEKWPHMDYKWALPDQMAQAGFYHQPSSSGEDRAMCFTCSVCLVCWEKTDEPWSEHERHSPLCPFVKGEYTQNVPLSITYATNPALPAPGLGFDIISNSDYANVLCTSCSQTGELSVWSIERHLKLMHTFHVPTLLNYIFEESFEWARVTAICVLPNARARTKVNYVYSAANYGGSGSSNGAGCNSGLNAVSGGKFGSVNAQHITSTSTLRAGVVGSKIVLGVSVRQSSGELALRMVVLNIVEVDRNSESSTSVSNDSGSVSSGGGQLMKSSSPMVNVSAENNATVTQVNLESKDDDNKAMTPYEKFADGFDSNGFVSALMTYNKNNGVSQNTDDASALYDFDLCTLSQILDHNLEEPLSLMVGSSNTVNVSNIGMGNEINKLVVDVEISHQNNNNNNNNNNEASSLGGNSSNNNNNIMINGGTNSSIDKMTAIEEIDCVVESWTSVKRMNALDGASSGVNGVNHMDQEVIEIAEILPTTPKCNQLLVKLLRTKAPEAEGSKIIMATVAKEEEEEDEEQRTDESMETDNNAEGNVAAQLLVFDYDDTEISNDYTLAMTFSRAKFPKQLCILPHFNSSPEHKDAGSICVVCADGTVEIYSLADFQGTMVIEEEGEHFESVVYCRNLDRLCCCSRQGGLLFYSLSDGENDSGDELLEMDDDCSTTLTQAADVSVTDGGRQLARTDLLGKTLLANESICMDAGSEAAAIPNSSSAAQGAHVQTSPSASSAPSTNANLLAYKTADLTLEDLKVFYALTQFDDKLTVYSAEVPSCWNDIGQVQNQRKQSQNMRHGGDERDFTRTWRLHNDATTWDEHIIELNLAQPVSLGHIDLKFTVYQQCSNPAAIQVTLLKQNNNGFGYRMKGPHSSYKPNVIDDNIDLSYIANENPVLSEEYLLAHNAEVLAGPIELSSCIDLCDQGGTATLTSPKLFKTRTRNFLLHIKTVSDPSKDGQNKTRGCDWLHEISISVRAVKSHSRISNGRMQRIAMLESNVLLVQLLRIASDPASSSLAKNLSLDILNWICFIRLNRFRSPKSERLKDQINKQTAANIQDLLAQQFECISLAEKHIEQLLRNCVIYSERSTAHKCVKLLIMLTEGATNLPAEVQDHTTLDYSFKAAIANTFPELPRAQCGSVVRWYMMLTCATSTAESHNSISEQAISLLKEIAAEIDKRWDPHCALLCTRFGLYGYPFEPEIFDFDFPNVSRQGPGSSVANVIRSSPTILPVTGLDIKRLSSIDGVDFRTFPYLIRGKSISNQLRGLLEVVQLHFNCVQTSEATRIDNIDSVGGNTGANVIIEDVMMMPKQVVAKEKGEEMLNDLVNDVECLVEEMKVDENEKQEKVDYDAFGLVNGFKDKTLSSLNSVKQTLKEEVKKEKADIMDKLTIAKFLPKLSLLESYMQKKINEHISFDDFGKLDNWDGNTTPPFNLEVEAPIVCSEATSESNMSQQATSAGGNILDDAADSEKITESLNANPCQPIAWHKLITPPPKQMIVIDRMHSGARRFVVLDFGQHILLTDLVIPSCDELTSLNIDIWCFDEETDCTRLVQVNDIQTKTLVLSDIQPAPICRYLKLTIIGRIGMSATKCKVPLGRFFGHPVVLEHDGYGDQLMKYIKHPTHSLQSQIKSLNALYEDVHCRYSLASSKLMDLLAPMRNSELSNVAHLQAFINKQREEELSGLENNNKVVSLYDECMLLQYQINVIRNVVLRLERALSPNSPTTTLLPGQSIPLTEDILRAASRDKLRVLSLSLVEVLLHFSIEYGIKNILPVHQRFNAATAKALFNALVVYGDAQLQLATCSLLVRMCCFQPWWGDFLADTFCSLFSAQNCKAFPQDRIFFLLTYLGRRSIAMGACRSIVIDAVLKTLVKLLAPISPHYKFQSEQPSTSANAADRNAAMGTTSDLQLITWLLLFLSVCLDDNERKDKSARWDFMSCEGDFTKARPSATPNIKQLRCFKKRIIQQNKYLAQPYADWGKKIYMIQNEHPGIFMELSTKSKGAGPTKSTSTGGKINMGSSGLCVGGPSSSSGNTSKSAASSSSPKNQAVPGTDGERDSNFDKGLKSLRMDNIKVVIRGLFALLLEMDFDCNMDQFLLTCKVIARLVAACRPSVQLCKIVTIAQLQQLVRLSVWNDQQHPWAVHAITCLLQDLLDADKQFRDNDVTPTNDTPRTMEAAIQETRDLFSDYTSTASSCTEALFETLMPTAREFYQEAVKYNYLPSLIECDDTEFDEMLNDIDMIERTKPVTKKESNALSKNTFNFFCKSISIALDSRLDVGLELHVETQLRRLTNRTSLDLYSSLPQVLTNEFVSPPPEAAPWPEYLTQLWSGTEYNGDNTYVMFKRVFDSILAELHLEDTWVHLEQVLQMWLTLNCELSDKPYTSGITHSDVPKIPFGANAVQGLLLALAWHSDIKLRTWCLGFQCLFLACNSLPIGDDADCTRINEAIVNDENFEKVLLRFFSGYGMSSSIITNRCAGPTICKHLHELLLWLHSKSETSGIPCKRRLKDILLHVVLQLVQPGGSISNQQGPIDAQNQLVRDLLMMPNDKGDLNIALKITESVSFLVYNNISNGERLQCQRGNENSPVGNNFSNLFANVLGSENPNKQNAAICDNSLIINLLKLSSHMVLTEMPRQPSEVTIPEEEELSNQSQTDETKAEQLNTEGHRSKVPCIADWVLRHFPTMKRLFGTLSQCSTNTFTMMSSGCFFSPKSNMILDDPQTIADAVFSLMITLNDKASHPRLVVVPICRYLEETTIPRNATLPRLPHSEPFLWYISKVLEVPAAVQTFTQLGGIQIICHSLVRLNKTIINMQPGLISLIMQHLTRNTRFKRNNHVAASSACKKTTTGTGTTCPTSAQPPRSAAQYDGLINFAPFSHIVSENDATQSAEVLISNPNNSHRRPRSPAWTYMFYPNETHVDLTITLPTAILLKEVQLVPHTTSLASCPSAVALELSRDYGLGSIPVGAPMATTGLTCIRLKLAKAEVATSIVLRLYKPKDCGNVGLVQIAVLGQTIFGNRMHGLRSPSPFIDSLATCSSPVQTAVLAAMDADSMLEDDAFAKTSIGWVRILSRCFHVAALQPDSKLADLIAAYPAAYPGFLEACCSLLNIMPMIPSLAQQHLETILLKLSAYNHEISLQLLRTLLWHTTPQVFKLSSDSVCDLIYEVVTGSTEHLLDKLRVLIDWVMQLHDNYSKQARCNNPQSGFVKVIASILWKVHTQQQLPELPLLISSSLFETCFEWVSSLEHDEPLKSSFDSLLCALCFIKPELFNQLLVKLGVKLEPPARGQTDDSKVQSGSAWFRREGSENLSVLLQRPTFLKTLALACQSPAAVYQMLDSGLPKLLAHAIYEYCMHLMPGSEPVASASTVPEDEPLANNQAAAAADDEDSSLTDFDKAEANVVYNTPLLSSSNVPKVLDFFAECCAEGPMRDWLGTMQGSVFWKPLLQLLCNTHAAQFSKTLPKEQSFIRLERATINFFTRVSACHPGNQENLTSLLIGAIICKPLRFSHGVRPTISGFTRQMVLQLLLENEHITVSVRSQQPLQRRDTLSAVLGGISSGASTSLPVAAVNNHPARRSSAHHMLFTVTTSTTCQEIMQNCVSVFSNLVYQQPTDQRAGETSASSSGVSSATAKSSESKMEKSSFLNFNNVEAGSMEFLTVGAAVAAKDKRIKDAKNQAAMNKDKETQTSSTNLASNIFNVDEFLLQSANAVNGNQLVIPEYSDIVLAGDATISQVLASLQNAGHSLSTPCISFDLVLQQRSDDTSEAKKTKAACTEPLPSPLQRFSSSGGLSLLAHYLPTVYPEHSGRKTTLVFSEKEKSPPLSDWVKLEPNDEIYEDLEDTLCEPAAKLATVSSVPQHSLAAFGLFLRLPAYSDVLLRDKMRAQCLLRLVLGVTGDGDGNDIYSLSLAPSLPTLPFEVFRQLLDSVPLSTDDGVLLRRVVIEVGAMHLVLNCLGIFSHQSHNNRIGAPISEPSPSGTSSGSGTIGNGSGNGSVSGTKTNAPEEATPSATSDDKSHMYWAKGTGFGTGSTTQSWNVEQALLRQKSEEEHVTVLLLVLASYINPGDCIPSEMCGEDILAYHDVRDVTGELPPIFQTLLQQSCLIPALSSYLRNDSVLDITRHIPLYRAILKLLRALSLSKMLVSLLQPSASGSGDSTPPIVELLTSMKTCVDTYAKRLKVNKKSNIKGQTHQLTFNIDDGDDEGLALLIPDIHETSVLVQKTTDADALINMLHTNEDGSAQLEPQSKSIEQRYLELMKKRQFDTFDMIVESDNNSFRFVVSHHFEKMVRLAGDRYHPSRVKRLAQEAVTLSTSLPLSFSSSVFVRCDTDRLDIMKVLITGPADTPYANGCFEFDVFFPPDYPNQPMLINLETTGRHSVRFNPNLYNDGKVCLSVLNTWHGRPEEKWNAQTSSFLQVLVSIQSLILVPEPYFNEPGFERSRGSPSGTNSSREYNSNIYQACVRWAMLEQIRSPSQCFKDVIHKHFWLKREEICAQIEGWIEELGKPQYTERASRTISFNSMVLRRHYRHLREELSKLKPPRGLEDLDAPFNPVATLPPMDVSAVAPSLAATNTVQAQPDDAIVTDITLLGENADECEADGDAEGDGEVDDGFKGFPQEEGLLTGEEEVVEILSDTENESSMWQ

Target ORF information:

RefSeq Version	NM_001275533.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275533.2

ORF Insert Sequence:

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCTCGCT GGGACTTCAT GAGCTGCGAG 
GGCGATTTTA CTAAGGCCAG ACCTAGTGCA ACTCCCAACA TCAAACAGCT ACGATGCTTT 
AAAAAACGCA TCATTCAGCA AAACAAGTAC TTGGCGCAGC CTTACGCTGA CTGGGGAAAG 
AAGATTTATA TGATACAGAA TGAGCATCCA GGCATTTTCA TGGAGCTGTC AACGAAATCA 
AAGGGCGCTG GACCAACCAA GTCGACCTCT ACAGGTGGCA AGATTAATAT GGGATCCTCT 
GGCCTGTGCG TGGGTGGTCC TTCAAGTTCC AGTGGCAATA CATCAAAGAG TGCGGCTTCC 
TCATCCTCTC CTAAGAATCA GGCTGTGCCG GGTACCGATG GCGAACGAGA CAGCAACTTT 
GACAAGGGAT TAAAATCACT TCGAATGGAT AACATTAAGG TGGTTATTCG CGGTCTCTTT 
GCACTGTTGC TTGAAATGGA CTTCGATTGC AATATGGACC AGTTTTTGCT CACTTGCAAA 
GTCATTGCCC GTTTGGTGGC TGCATGCAGA CCGTCGGTAC AGCTGTGTAA GATTGTGACC 
ATCGCCCAAC TGCAGCAACT TGTACGTCTA TCCGTGTGGA ATGATCAGCA GCATCCCTGG 
GCTGTTCATG CCATTACGTG TTTACTGCAA GATCTTTTGG ATGCGGACAA GCAGTTCCGG 
GACAATGATG TCACACCTAC AAATGACACG CCGCGTACAA TGGAAGCTGC GATCCAGGAA 
ACACGGGATC TTTTCAGCGA CTATACTTCC ACTGCTTCCT CGTGTACTGA GGCACTATTT 
GAGACGCTGA TGCCGACAGC AAGAGAATTT TATCAAGAGG CCGTCAAATA TAATTACCTA 
CCTTCATTAA TTGAATGCGA TGACACGGAA TTCGATGAAA TGCTAAACGA CATTGACATG 
ATTGAACGAA CGAAGCCAGT TACCAAAAAG GAAAGTAATG CCCTGTCTAA GAATACATTC 
AATTTCTTCT GCAAGTCTAT CTCGATTGCT CTGGATTCAC GATTGGACGT GGGTCTGGAA 
TTGCACGTAG AAACTCAGCT GAGAAGGCTA ACAAATCGCA CATCCCTGGA TCTCTATTCA 
TCGCTTCCTC AGGTGTTGAC CAATGAGTTT GTCTCTCCAC CACCTGAGGC TGCTCCTTGG 
CCAGAATATT TGACTCAACT TTGGTCAGGC ACGGAATACA ACGGTGACAA TACTTATGTG 
ATGTTTAAAA GGGTCTTTGA CTCCATCCTG GCGGAATTGC ACCTTGAAGA CACGTGGGTG 
CACTTGGAGC AGGTGCTGCA GATGTGGCTG ACGCTAAATT GCGAGCTCTC GGACAAGCCA 
TACACCAGTG GCATCACTCA CTCGGATGTT CCAAAAATTC CGTTTGGAGC AAATGCGGTG 
CAGGGATTGC TCCTGGCTTT GGCTTGGCAC AGTGATATAA AACTGCGGAC CTGGTGTCTT 
GGCTTTCAGT GCCTGTTTCT CGCTTGCAAC TCATTGCCTA TCGGAGATGA TGCGGATTGT 
ACGCGTATCA ACGAGGCTAT CGTGAATGAT GAAAACTTTG AAAAAGTGCT GCTACGATTC 
TTTTCCGGTT ATGGCATGAG CTCATCTATC ATCACCAACC GATGCGCTGG ACCTACTATT 
TGCAAGCACT TGCATGAGTT ATTGCTCTGG CTGCACAGCA AAAGCGAGAC GAGTGGCATT 
CCGTGCAAGC GTCGACTTAA GGACATTTTG TTGCATGTTG TGCTTCAGTT GGTGCAGCCT 
GGTGGCTCTA TTAGCAACCA GCAGGGTCCA ATTGATGCTC AAAACCAGCT GGTTCGTGAT 
TTGCTTATGA TGCCCAATGA TAAGGGGGAC CTCAACATTG CGCTTAAAAT CACAGAAAGT 
GTTTCTTTCC TGGTGTACAA CAACATATCC AATGGCGAGA GGCTACAATG TCAGCGTGGA 
AATGAAAACA GCCCAGTTGG CAACAATTTT AGCAATTTGT TTGCCAACGT TCTGGGATCA 
GAAAATCCCA ACAAGCAGAA TGCCGCAATT TGCGACAACT CACTTATTAT AAATCTGCTG 
AAACTATCGT CACACATGGT ATTGACAGAG ATGCCCCGGC AACCAAGTGA GGTTACCATT 
CCCGAGGAGG AGGAACTTTC CAATCAGAGT CAAACGGATG AGACTAAAGC GGAGCAGTTA 
AACACGGAAG GACATCGCAG CAAAGTGCCT TGCATTGCAG ATTGGGTACT CCGCCATTTC 
CCCACAATGA AGCGACTTTT TGGAACGCTT TCCCAGTGCT CGACAAACAC CTTCACCATG 
ATGTCCTCTG GCTGTTTCTT TTCACCGAAG TCCAATATGA TTTTAGATGA CCCGCAGACA 
ATTGCAGACG CCGTCTTTAG TTTGATGATT ACTCTCAACG ACAAGGCTTC GCATCCCCGA 
CTTGTTGTGG TGCCCATTTG CCGATACCTT GAAGAAACCA CTATTCCGAG AAATGCAACG 
CTGCCGCGTC TGCCGCATTC TGAACCTTTC CTGTGGTACA TCTCGAAGGT ATTGGAAGTA 
CCAGCGGCAG TGCAAACGTT CACCCAATTG GGCGGAATTC AGATTATATG TCATAGCCTG 
GTGCGACTTA ATAAAACTAT CATCAACATG CAACCAGGAT TGATTTCTTT GATTATGCAA 
CACTTGACGC GCAATACGCG CTTTAAGCGT AATAATCATG TGGCGGCAAG TAGTGCTTGC 
AAAAAGACAA CGACTGGTAC GGGAACCACA TGTCCCACGT CAGCACAGCC GCCGAGAAGT 
GCTGCTCAGT ACGATGGTCT GATCAACTTT GCACCCTTCT CGCATATTGT CTCCGAAAAT 
GATGCGACAC AAAGTGCAGA GGTACTAATA TCAAATCCAA ATAATTCACA TCGAAGACCG 
CGATCGCCAG CCTGGACCTA TATGTTCTAT CCAAATGAGA CGCATGTGGA TCTGACTATA 
ACGTTGCCCA CAGCAATTTT GTTGAAAGAG GTGCAGCTGG TGCCGCACAC AACTAGTTTG 
GCATCCTGTC CATCTGCGGT GGCTCTAGAG CTGTCTAGAG ATTACGGCCT TGGTTCGATC 
CCAGTGGGTG CTCCCATGGC CACCACTGGT CTGACCTGCA TTAGACTAAA GCTGGCCAAG 
GCAGAGGTGG CTACCAGCAT TGTTCTGAGG CTGTACAAAC CCAAGGACTG TGGAAATGTG 
GGTCTCGTAC AAATCGCTGT GCTTGGTCAG ACAATCTTCG GAAATCGCAT GCATGGCCTT 
CGCTCACCGA GTCCGTTCAT TGACTCCCTA GCAACGTGCT CCTCGCCAGT GCAAACGGCA 
GTTTTAGCCG CAATGGATGC GGATTCCATG CTTGAAGATG ATGCTTTTGC GAAGACTAGC 
ATTGGCTGGG TTCGCATTCT GTCGCGCTGT TTCCATGTGG CCGCTTTACA ACCAGACAGT 
AAGCTGGCTG ATCTTATCGC CGCCTATCCG GCTGCATATC CCGGCTTTTT AGAGGCCTGC 
TGCTCATTGC TAAACATAAT GCCAATGATT CCTAGTCTTG CACAGCAACA CTTGGAGACC 
ATTTTGTTAA AATTAAGTGC ATACAACCAC GAGATCAGTT TGCAACTTCT TAGGACGCTC 
CTGTGGCACA CCACTCCGCA AGTTTTTAAA CTCTCCAGCG ATTCGGTGTG TGATCTTATT 
TATGAAGTAG TTACCGGATC GACGGAGCAC CTACTGGACA AACTACGCGT GCTTATCGAC 
TGGGTAATGC AATTGCACGA CAACTACAGC AAGCAGGCGC GCTGTAATAA CCCACAAAGT 
GGATTTGTTA AGGTGATTGC TTCGATTCTG TGGAAAGTGC ATACCCAGCA ACAATTGCCT 
GAGTTACCGC TTCTCATTTC ATCTTCGCTG TTCGAGACCT GTTTTGAATG GGTCTCTTCG 
CTGGAGCACG ATGAGCCACT GAAGAGCAGT TTTGACTCCT TGTTGTGTGC ATTGTGCTTC 
ATTAAGCCAG AACTGTTTAA CCAGCTGTTG GTCAAGCTGG GAGTTAAGCT GGAACCTCCA 
GCTCGAGGTC AAACTGATGA CAGCAAAGTG CAAAGTGGTA GTGCTTGGTT CCGCCGTGAG 
GGTAGCGAAA ACCTTAGTGT TTTGCTACAG CGTCCCACAT TCCTAAAGAC TCTAGCCTTG 
GCCTGCCAGT CCCCTGCAGC AGTTTACCAG ATGCTAGACT CCGGACTTCC AAAGCTATTG 
GCCCACGCCA TTTACGAGTA TTGCATGCAT CTCATGCCGG GATCGGAGCC AGTAGCTTCT 
GCATCTACAG TGCCGGAAGA CGAACCGTTA GCCAACAATC AGGCAGCCGC TGCAGCAGAT 
GACGAGGACT CGTCTCTTAC GGACTTTGAC AAGGCCGAGG CAAATGTGGT TTATAATACT 
CCACTGCTAA GTAGCTCGAA TGTACCCAAA GTGCTAGATT TCTTTGCCGA ATGTTGTGCC 
GAAGGTCCAA TGCGTGATTG GTTAGGTACT ATGCAGGGCT CCGTATTTTG GAAGCCTCTG 
CTGCAGTTGC TCTGTAACAC ACATGCCGCC CAGTTCAGCA AGACGTTGCC TAAGGAACAA 
TCGTTTATCC GCCTGGAGAG GGCCACAATC AACTTCTTTA CGCGAGTTAG CGCCTGCCAT 
CCCGGAAACC AGGAAAATCT TACATCGTTG CTCATTGGAG CCATAATTTG CAAACCATTA 
AGATTTTCGC ATGGAGTTCG ACCAACTATA TCTGGATTTA CTAGACAGAT GGTGCTGCAG 
CTCCTCCTCG AGAATGAACA CATCACAGTC TCTGTTAGAA GTCAGCAGCC CTTGCAACGG 
AGGGATACCC TCTCCGCCGT TCTAGGCGGG ATTTCGAGTG GTGCAAGTAC TAGCTTACCC 
GTAGCAGCAG TCAACAATCA TCCGGCCAGG AGGAGCAGCG CCCACCACAT GCTATTCACG 
GTGACCACTA GCACCACGTG CCAAGAGATT ATGCAGAATT GCGTTAGTGT GTTTAGTAAT 
CTAGTTTATC AACAACCAAC AGATCAGCGA GCTGGCGAGA CCTCTGCCTC GAGCAGCGGC 
GTATCGAGTG CAACTGCCAA ATCCAGTGAG AGCAAAATGG AAAAGAGCAG CTTCCTGAAC 
TTTAACAACG TAGAAGCGGG TAGCATGGAG TTCCTTACCG TTGGTGCTGC TGTGGCAGCC 
AAGGACAAGC GCATAAAAGA TGCTAAGAAC CAAGCAGCCA TGAACAAGGA CAAGGAGACT 
CAGACCAGCT CAACGAACTT GGCTTCCAAT ATATTCAATG TGGATGAGTT CTTGTTGCAA 
TCGGCGAATG CCGTTAATGG TAACCAGTTG GTCATTCCAG AGTATTCTGA TATTGTTCTA 
GCTGGAGATG CCACCATCTC GCAGGTTTTG GCCTCGCTTC AGAATGCAGG ACATTCTCTT 
TCGACGCCCT GTATATCGTT TGATCTGGTA CTTCAACAGC GCTCTGATGA CACAAGCGAG 
GCAAAGAAGA CCAAAGCTGC CTGCACTGAG CCGTTGCCAT CACCACTGCA ACGATTTTCC 
AGCAGTGGTG GTCTATCTTT ACTCGCTCAC TACCTGCCCA CCGTATATCC GGAACACTCA 
GGTCGCAAGA CCACCTTGGT GTTTAGCGAG AAGGAGAAAA GTCCCCCGCT CTCTGACTGG 
GTGAAATTGG AACCGAACGA CGAGATCTAT GAGGATTTGG AGGACACTCT CTGTGAGCCA 
GCAGCTAAGC TGGCAACGGT CAGTTCGGTG CCGCAGCACT CGCTGGCCGC ATTTGGATTA 
TTCCTGCGCC TGCCCGCCTA CTCTGATGTC TTGCTGCGAG ACAAGATGCG GGCGCAGTGT 
CTTCTCCGCT TAGTGCTCGG AGTCACGGGA GATGGGGATG GCAATGATAT ATACAGTCTG 
TCGCTGGCAC CCTCCTTGCC TACATTACCC TTCGAGGTCT TCCGTCAGCT TCTTGATAGT 
GTGCCGTTAT CCACCGACGA CGGTGTTTTG CTACGACGCG TGGTAATCGA AGTGGGGGCC 
ATGCACCTAG TGCTTAATTG CCTCGGCATT TTCTCGCATC AGTCGCACAA CAACAGGATT 
GGAGCACCGA TTAGCGAACC ATCGCCAAGT GGAACCAGTA GTGGTAGCGG CACCATTGGC 
AATGGAAGTG GAAACGGAAG TGTAAGCGGT ACCAAAACTA ATGCACCAGA AGAGGCCACA 
CCTAGTGCCA CGTCGGACGA CAAAAGCCAC ATGTACTGGG CCAAGGGCAC TGGTTTCGGT 
ACCGGTTCTA CTACTCAGTC GTGGAATGTG GAGCAGGCGC TTCTTCGGCA AAAGAGCGAG 
GAGGAGCATG TCACAGTGTT GCTGCTTGTT CTAGCCAGTT ACATAAATCC GGGTGACTGT 
ATCCCAAGTG AAATGTGCGG AGAGGACATC CTAGCTTATC ATGATGTCCG AGATGTTACC 
GGCGAGTTGC CGCCCATCTT CCAAACCCTA CTGCAGCAGT CTTGCTTAAT CCCCGCCCTC 
AGCTCCTATC TGCGCAACGA TTCAGTGCTT GATATCACGC GCCACATTCC GCTATACCGT 
GCCATACTCA AACTTCTGCG TGCCCTATCG CTGTCTAAGA TGCTTGTCTC GTTGCTCCAA 
CCATCTGCCA GTGGCAGTGG GGACAGCACG CCACCCATAG TGGAGTTGCT CACAAGCATG 
AAGACCTGTG TGGATACCTA CGCCAAACGG TTAAAAGTTA ACAAGAAGTC AAACATAAAG 
GGACAGACAC ATCAGTTGAC CTTTAACATC GATGATGGCG ACGATGAGGG ATTAGCTTTG 
CTTATTCCGG ATATCCATGA AACCAGTGTG CTGGTGCAAA AAACCACAGA TGCTGATGCG 
CTAATCAACA TGTTGCATAC CAACGAAGAC GGCAGCGCAC AGCTAGAGCC CCAGAGCAAA 
TCAATTGAAC AGCGCTACTT AGAGCTAATG AAGAAGCGCC AGTTCGATAC GTTTGACATG 
ATTGTGGAGT CGGATAACAA CAGTTTCCGC TTTGTGGTCT CGCATCACTT CGAAAAAATG 
GTTCGGCTCG CTGGCGATCG TTACCATCCG TCGCGGGTCA AGCGACTTGC CCAGGAGGCA 
GTTACACTGT CCACCAGCCT ACCCCTCAGC TTTAGTAGTT CTGTATTTGT ACGCTGTGAT 
ACTGATAGAT TGGACATCAT GAAGGTACTC ATAACTGGTC CGGCGGACAC GCCTTACGCC 
AACGGGTGCT TTGAGTTTGA TGTCTTCTTT CCGCCGGACT ACCCTAACCA GCCCATGCTC 
ATCAATCTGG AGACGACGGG TCGCCATTCG GTGCGCTTTA ACCCAAACCT CTATAATGAC 
GGCAAAGTTT GCCTGTCAGT TCTCAACACC TGGCATGGTC GGCCTGAGGA GAAATGGAAT 
GCACAGACTT CAAGCTTCCT GCAGGTACTC GTTTCCATCC AGTCCCTGAT CCTCGTTCCC 
GAGCCCTATT TCAATGAACC GGGCTTTGAA AGAAGTCGTG GCAGTCCAAG TGGTACCAAT 
TCATCGCGAG AATATAATAG CAATATATAT CAGGCCTGTG TGCGGTGGGC TATGTTGGAG 
CAAATACGGA GTCCAAGTCA GTGCTTTAAA GATGTCATAC ACAAGCACTT CTGGTTGAAG 
AGAGAGGAGA TTTGCGCCCA GATAGAGGGC TGGATCGAGG AACTCGGTAA GCCGCAGTAC 
ACCGAGAGGG CCAGTCGCAC TATATCGTTC AACTCAATGG TCCTGCGCCG CCACTATCGC 
CACCTGCGTG AGGAGTTGTC GAAACTGAAG CCGCCACGTG GCCTCGAGGA CTTGGATGCG 
CCCTTTAATC CTGTGGCCAC GCTACCACCG ATGGATGTGT CCGCCGTGGC TCCTTCACTA 
GCAGCCACAA ATACCGTCCA AGCGCAACCG GATGATGCCA TAGTGACCGA TATTACCCTG 
CTCGGCGAGA ATGCGGATGA ATGCGAAGCT GATGGCGATG CCGAAGGCGA TGGAGAAGTG 
GACGATGGCT TTAAGGGTTT CCCGCAAGAA GAGGGTTTGC TGACGGGCGA GGAAGAGGTC 
GTGGAAATTT TAAGTGATAC GGAGAACGAG AGCAGTATGT GGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bruce ( NM_001275532.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262461.1 Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant C, mRNA.

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CloneID	OFa08256
Clone ID Related Accession (Same CDS sequence)	NM_001275532.2
Accession Version	NM_001275532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BIR repeat containing ubiquitin-conjugating enzyme, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275532.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCGTCAAAT ATAATTACCT ACCTTCATTA 
ATTGAATGCG ATGACACGGA ATTCGATGAA ATGCTAAACG ACATTGACAT GATTGAACGA 
ACGAAGCCAG TTACCAAAAA GGAAAGTAAT GCCCTGTCTA AGAATACATT CAATTTCTTC 
TGCAAGTCTA TCTCGATTGC TCTGGATTCA CGATTGGACG TGGGTCTGGA ATTGCACGTA 
GAAACTCAGC TGAGAAGGCT AACAAATCGC ACATCCCTGG ATCTCTATTC ATCGCTTCCT 
CAGGTGTTGA CCAATGAGTT TGTCTCTCCA CCACCTGAGG CTGCTCCTTG GCCAGAATAT 
TTGACTCAAC TTTGGTCAGG CACGGAATAC AACGGTGACA ATACTTATGT GATGTTTAAA 
AGGGTCTTTG ACTCCATCCT GGCGGAATTG CACCTTGAAG ACACGTGGGT GCACTTGGAG 
CAGGTGCTGC AGATGTGGCT GACGCTAAAT TGCGAGCTCT CGGACAAGCC ATACACCAGT 
GGCATCACTC ACTCGGATGT TCCAAAAATT CCGTTTGGAG CAAATGCGGT GCAGGGATTG 
CTCCTGGCTT TGGCTTGGCA CAGTGATATA AAACTGCGGA CCTGGTGTCT TGGCTTTCAG 
TGCCTGTTTC TCGCTTGCAA CTCATTGCCT ATCGGAGATG ATGCGGATTG TACGCGTATC 
AACGAGGCTA TCGTGAATGA TGAAAACTTT GAAAAAGTGC TGCTACGATT CTTTTCCGGT 
TATGGCATGA GCTCATCTAT CATCACCAAC CGATGCGCTG GACCTACTAT TTGCAAGCAC 
TTGCATGAGT TATTGCTCTG GCTGCACAGC AAAAGCGAGA CGAGTGGCAT TCCGTGCAAG 
CGTCGACTTA AGGACATTTT GTTGCATGTT GTGCTTCAGT TGGTGCAGCC TGGTGGCTCT 
ATTAGCAACC AGCAGGGTCC AATTGATGCT CAAAACCAGC TGGTTCGTGA TTTGCTTATG 
ATGCCCAATG ATAAGGGGGA CCTCAACATT GCGCTTAAAA TCACAGAAAG TGTTTCTTTC 
CTGGTGTACA ACAACATATC CAATGGCGAG AGGCTACAAT GTCAGCGTGG AAATGAAAAC 
AGCCCAGTTG GCAACAATTT TAGCAATTTG TTTGCCAACG TTCTGGGATC AGAAAATCCC 
AACAAGCAGA ATGCCGCAAT TTGCGACAAC TCACTTATTA TAAATCTGCT GAAACTATCG 
TCACACATGG TATTGACAGA GATGCCCCGG CAACCAAGTG AGGTTACCAT TCCCGAGGAG 
GAGGAACTTT CCAATCAGAG TCAAACGGAT GAGACTAAAG CGGAGCAGTT AAACACGGAA 
GGACATCGCA GCAAAGTGCC TTGCATTGCA GATTGGGTAC TCCGCCATTT CCCCACAATG 
AAGCGACTTT TTGGAACGCT TTCCCAGTGC TCGACAAACA CCTTCACCAT GATGTCCTCT 
GGCTGTTTCT TTTCACCGAA GTCCAATATG ATTTTAGATG ACCCGCAGAC AATTGCAGAC 
GCCGTCTTTA GTTTGATGAT TACTCTCAAC GACAAGGCTT CGCATCCCCG ACTTGTTGTG 
GTGCCCATTT GCCGATACCT TGAAGAAACC ACTATTCCGA GAAATGCAAC GCTGCCGCGT 
CTGCCGCATT CTGAACCTTT CCTGTGGTAC ATCTCGAAGG TATTGGAAGT ACCAGCGGCA 
GTGCAAACGT TCACCCAATT GGGCGGAATT CAGATTATAT GTCATAGCCT GGTGCGACTT 
AATAAAACTA TCATCAACAT GCAACCAGGA TTGATTTCTT TGATTATGCA ACACTTGACG 
CGCAATACGC GCTTTAAGCG TAATAATCAT GTGGCGGCAA GTAGTGCTTG CAAAAAGACA 
ACGACTGGTA CGGGAACCAC ATGTCCCACG TCAGCACAGC CGCCGAGAAG TGCTGCTCAG 
TACGATGGTC TGATCAACTT TGCACCCTTC TCGCATATTG TCTCCGAAAA TGATGCGACA 
CAAAGTGCAG AGGTACTAAT ATCAAATCCA AATAATTCAC ATCGAAGACC GCGATCGCCA 
GCCTGGACCT ATATGTTCTA TCCAAATGAG ACGCATGTGG ATCTGACTAT AACGTTGCCC 
ACAGCAATTT TGTTGAAAGA GGTGCAGCTG GTGCCGCACA CAACTAGTTT GGCATCCTGT 
CCATCTGCGG TGGCTCTAGA GCTGTCTAGA GATTACGGCC TTGGTTCGAT CCCAGTGGGT 
GCTCCCATGG CCACCACTGG TCTGACCTGC ATTAGACTAA AGCTGGCCAA GGCAGAGGTG 
GCTACCAGCA TTGTTCTGAG GCTGTACAAA CCCAAGGACT GTGGAAATGT GGGTCTCGTA 
CAAATCGCTG TGCTTGGTCA GACAATCTTC GGAAATCGCA TGCATGGCCT TCGCTCACCG 
AGTCCGTTCA TTGACTCCCT AGCAACGTGC TCCTCGCCAG TGCAAACGGC AGTTTTAGCC 
GCAATGGATG CGGATTCCAT GCTTGAAGAT GATGCTTTTG CGAAGACTAG CATTGGCTGG 
GTTCGCATTC TGTCGCGCTG TTTCCATGTG GCCGCTTTAC AACCAGACAG TAAGCTGGCT 
GATCTTATCG CCGCCTATCC GGCTGCATAT CCCGGCTTTT TAGAGGCCTG CTGCTCATTG 
CTAAACATAA TGCCAATGAT TCCTAGTCTT GCACAGCAAC ACTTGGAGAC CATTTTGTTA 
AAATTAAGTG CATACAACCA CGAGATCAGT TTGCAACTTC TTAGGACGCT CCTGTGGCAC 
ACCACTCCGC AAGTTTTTAA ACTCTCCAGC GATTCGGTGT GTGATCTTAT TTATGAAGTA 
GTTACCGGAT CGACGGAGCA CCTACTGGAC AAACTACGCG TGCTTATCGA CTGGGTAATG 
CAATTGCACG ACAACTACAG CAAGCAGGCG CGCTGTAATA ACCCACAAAG TGGATTTGTT 
AAGGTGATTG CTTCGATTCT GTGGAAAGTG CATACCCAGC AACAATTGCC TGAGTTACCG 
CTTCTCATTT CATCTTCGCT GTTCGAGACC TGTTTTGAAT GGGTCTCTTC GCTGGAGCAC 
GATGAGCCAC TGAAGAGCAG TTTTGACTCC TTGTTGTGTG CATTGTGCTT CATTAAGCCA 
GAACTGTTTA ACCAGCTGTT GGTCAAGCTG GGAGTTAAGC TGGAACCTCC AGCTCGAGGT 
CAAACTGATG ACAGCAAAGT GCAAAGTGGT AGTGCTTGGT TCCGCCGTGA GGGTAGCGAA 
AACCTTAGTG TTTTGCTACA GCGTCCCACA TTCCTAAAGA CTCTAGCCTT GGCCTGCCAG 
TCCCCTGCAG CAGTTTACCA GATGCTAGAC TCCGGACTTC CAAAGCTATT GGCCCACGCC 
ATTTACGAGT ATTGCATGCA TCTCATGCCG GGATCGGAGC CAGTAGCTTC TGCATCTACA 
GTGCCGGAAG ACGAACCGTT AGCCAACAAT CAGGCAGCCG CTGCAGCAGA TGACGAGGAC 
TCGTCTCTTA CGGACTTTGA CAAGGCCGAG GCAAATGTGG TTTATAATAC TCCACTGCTA 
AGTAGCTCGA ATGTACCCAA AGTGCTAGAT TTCTTTGCCG AATGTTGTGC CGAAGGTCCA 
ATGCGTGATT GGTTAGGTAC TATGCAGGGC TCCGTATTTT GGAAGCCTCT GCTGCAGTTG 
CTCTGTAACA CACATGCCGC CCAGTTCAGC AAGACGTTGC CTAAGGAACA ATCGTTTATC 
CGCCTGGAGA GGGCCACAAT CAACTTCTTT ACGCGAGTTA GCGCCTGCCA TCCCGGAAAC 
CAGGAAAATC TTACATCGTT GCTCATTGGA GCCATAATTT GCAAACCATT AAGATTTTCG 
CATGGAGTTC GACCAACTAT ATCTGGATTT ACTAGACAGA TGGTGCTGCA GCTCCTCCTC 
GAGAATGAAC ACATCACAGT CTCTGTTAGA AGTCAGCAGC CCTTGCAACG GAGGGATACC 
CTCTCCGCCG TTCTAGGCGG GATTTCGAGT GGTGCAAGTA CTAGCTTACC CGTAGCAGCA 
GTCAACAATC ATCCGGCCAG GAGGAGCAGC GCCCACCACA TGCTATTCAC GGTGACCACT 
AGCACCACGT GCCAAGAGAT TATGCAGAAT TGCGTTAGTG TGTTTAGTAA TCTAGTTTAT 
CAACAACCAA CAGATCAGCG AGCTGGCGAG ACCTCTGCCT CGAGCAGCGG CGTATCGAGT 
GCAACTGCCA AATCCAGTGA GAGCAAAATG GAAAAGAGCA GCTTCCTGAA CTTTAACAAC 
GTAGAAGCGG GTAGCATGGA GTTCCTTACC GTTGGTGCTG CTGTGGCAGC CAAGGACAAG 
CGCATAAAAG ATGCTAAGAA CCAAGCAGCC ATGAACAAGG ACAAGGAGAC TCAGACCAGC 
TCAACGAACT TGGCTTCCAA TATATTCAAT GTGGATGAGT TCTTGTTGCA ATCGGCGAAT 
GCCGTTAATG GTAACCAGTT GGTCATTCCA GAGTATTCTG ATATTGTTCT AGCTGGAGAT 
GCCACCATCT CGCAGGTTTT GGCCTCGCTT CAGAATGCAG GACATTCTCT TTCGACGCCC 
TGTATATCGT TTGATCTGGT ACTTCAACAG CGCTCTGATG ACACAAGCGA GGCAAAGAAG 
ACCAAAGCTG CCTGCACTGA GCCGTTGCCA TCACCACTGC AACGATTTTC CAGCAGTGGT 
GGTCTATCTT TACTCGCTCA CTACCTGCCC ACCGTATATC CGGAACACTC AGGTCGCAAG 
ACCACCTTGG TGTTTAGCGA GAAGGAGAAA AGTCCCCCGC TCTCTGACTG GGTGAAATTG 
GAACCGAACG ACGAGATCTA TGAGGATTTG GAGGACACTC TCTGTGAGCC AGCAGCTAAG 
CTGGCAACGG TCAGTTCGGT GCCGCAGCAC TCGCTGGCCG CATTTGGATT ATTCCTGCGC 
CTGCCCGCCT ACTCTGATGT CTTGCTGCGA GACAAGATGC GGGCGCAGTG TCTTCTCCGC 
TTAGTGCTCG GAGTCACGGG AGATGGGGAT GGCAATGATA TATACAGTCT GTCGCTGGCA 
CCCTCCTTGC CTACATTACC CTTCGAGGTC TTCCGTCAGC TTCTTGATAG TGTGCCGTTA 
TCCACCGACG ACGGTGTTTT GCTACGACGC GTGGTAATCG AAGTGGGGGC CATGCACCTA 
GTGCTTAATT GCCTCGGCAT TTTCTCGCAT CAGTCGCACA ACAACAGGAT TGGAGCACCG 
ATTAGCGAAC CATCGCCAAG TGGAACCAGT AGTGGTAGCG GCACCATTGG CAATGGAAGT 
GGAAACGGAA GTGTAAGCGG TACCAAAACT AATGCACCAG AAGAGGCCAC ACCTAGTGCC 
ACGTCGGACG ACAAAAGCCA CATGTACTGG GCCAAGGGCA CTGGTTTCGG TACCGGTTCT 
ACTACTCAGT CGTGGAATGT GGAGCAGGCG CTTCTTCGGC AAAAGAGCGA GGAGGAGCAT 
GTCACAGTGT TGCTGCTTGT TCTAGCCAGT TACATAAATC CGGGTGACTG TATCCCAAGT 
GAAATGTGCG GAGAGGACAT CCTAGCTTAT CATGATGTCC GAGATGTTAC CGGCGAGTTG 
CCGCCCATCT TCCAAACCCT ACTGCAGCAG TCTTGCTTAA TCCCCGCCCT CAGCTCCTAT 
CTGCGCAACG ATTCAGTGCT TGATATCACG CGCCACATTC CGCTATACCG TGCCATACTC 
AAACTTCTGC GTGCCCTATC GCTGTCTAAG ATGCTTGTCT CGTTGCTCCA ACCATCTGCC 
AGTGGCAGTG GGGACAGCAC GCCACCCATA GTGGAGTTGC TCACAAGCAT GAAGACCTGT 
GTGGATACCT ACGCCAAACG GTTAAAAGTT AACAAGAAGT CAAACATAAA GGGACAGACA 
CATCAGTTGA CCTTTAACAT CGATGATGGC GACGATGAGG GATTAGCTTT GCTTATTCCG 
GATATCCATG AAACCAGTGT GCTGGTGCAA AAAACCACAG ATGCTGATGC GCTAATCAAC 
ATGTTGCATA CCAACGAAGA CGGCAGCGCA CAGCTAGAGC CCCAGAGCAA ATCAATTGAA 
CAGCGCTACT TAGAGCTAAT GAAGAAGCGC CAGTTCGATA CGTTTGACAT GATTGTGGAG 
TCGGATAACA ACAGTTTCCG CTTTGTGGTC TCGCATCACT TCGAAAAAAT GGTTCGGCTC 
GCTGGCGATC GTTACCATCC GTCGCGGGTC AAGCGACTTG CCCAGGAGGC AGTTACACTG 
TCCACCAGCC TACCCCTCAG CTTTAGTAGT TCTGTATTTG TACGCTGTGA TACTGATAGA 
TTGGACATCA TGAAGGTACT CATAACTGGT CCGGCGGACA CGCCTTACGC CAACGGGTGC 
TTTGAGTTTG ATGTCTTCTT TCCGCCGGAC TACCCTAACC AGCCCATGCT CATCAATCTG 
GAGACGACGG GTCGCCATTC GGTGCGCTTT AACCCAAACC TCTATAATGA CGGCAAAGTT 
TGCCTGTCAG TTCTCAACAC CTGGCATGGT CGGCCTGAGG AGAAATGGAA TGCACAGACT 
TCAAGCTTCC TGCAGGTACT CGTTTCCATC CAGTCCCTGA TCCTCGTTCC CGAGCCCTAT 
TTCAATGAAC CGGGCTTTGA AAGAAGTCGT GGCAGTCCAA GTGGTACCAA TTCATCGCGA 
GAATATAATA GCAATATATA TCAGGCCTGT GTGCGGTGGG CTATGTTGGA GCAAATACGG 
AGTCCAAGTC AGTGCTTTAA AGATGTCATA CACAAGCACT TCTGGTTGAA GAGAGAGGAG 
ATTTGCGCCC AGATAGAGGG CTGGATCGAG GAACTCGGTA AGCCGCAGTA CACCGAGAGG 
GCCAGTCGCA CTATATCGTT CAACTCAATG GTCCTGCGCC GCCACTATCG CCACCTGCGT 
GAGGAGTTGT CGAAACTGAA GCCGCCACGT GGCCTCGAGG ACTTGGATGC GCCCTTTAAT 
CCTGTGGCCA CGCTACCACC GATGGATGTG TCCGCCGTGG CTCCTTCACT AGCAGCCACA 
AATACCGTCC AAGCGCAACC GGATGATGCC ATAGTGACCG ATATTACCCT GCTCGGCGAG 
AATGCGGATG AATGCGAAGC TGATGGCGAT GCCGAAGGCG ATGGAGAAGT GGACGATGGC 
TTTAAGGGTT TCCCGCAAGA AGAGGGTTTG CTGACGGGCG AGGAAGAGGT CGTGGAAATT 
TTAAGTGATA CGGAGAACGA GAGCAGTATG TGGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262461.1
CDS	167..14725
Translation	MATEQHHQQRVLKQDGYLDLGDCNSAPSRPFEMRNVVYHPQLNVLLLFDNGSTVKVLDANSGVILQTYHLSSNGDSIVHGRYMPLQDKILFWDGHNLGLRGDYNGVLLLDTILQAPIGQNDDYIKLEIILSEAIIFQNCITELESEGLECPCDISNELTQKINQAQLKAKKGIKAQRWNTICLEVPYSSLKLVSNNMVILLKRLERHIPVLAIASAINERLTDMMMGSRVPDFGWNFSNFQRVLMHSEAVRRQTFEKWPHMDYKWALPDQMAQAGFYHQPSSSGEDRAMCFTCSVCLVCWEKTDEPWSEHERHSPLCPFVKGEYTQNVPLSITYATNPALPAPGLGFDIISNSDYANVLCTSCSQTGELSVWSIERHLKLMHTFHVPTLLNYIFEESFEWARVTAICVLPNARARTKVNYVYSAANYGGSGSSNGAGCNSGLNAVSGGKFGSVNAQHITSTSTLRAGVVGSKIVLGVSVRQSSGELALRMVVLNIVEVDRNSESSTSVSNDSGSVSSGGGQLMKSSSPMVNVSAENNATVTQVNLESKDDDNKAMTPYEKFADGFDSNGFVSALMTYNKNNGVSQNTDDASALYDFDLCTLSQILDHNLEEPLSLMVGSSNTVNVSNIGMGNEINKLVVDVEISHQNNNNNNNNNNEASSLGGNSSNNNNNIMINGGTNSSIDKMTAIEEIDCVVESWTSVKRMNALDGASSGVNGVNHMDQEVIEIAEILPTTPKCNQLLVKLLRTKAPEAEGSKIIMATVAKEEEEEDEEQRTDESMETDNNAEGNVAAQLLVFDYDDTEISNDYTLAMTFSRAKFPKQLCILPHFNSSPEHKDAGSICVVCADGTVEIYSLADFQGTMVIEEEGEHFESVVYCRNLDRLCCCSRQGGLLFYSLSDGENDSGDELLEMDDDCSTTLTQAADVSVTDGGRQLARTDLLGKTLLANESICMDAGSEAAAIPNSSSAAQGAHVQTSPSASSAPSTNANLLAYKTADLTLEDLKVFYALTQFDDKLTVYSAEVPSCWNDIGQVQNQRKQSQNMRHGGDERDFTRTWRLHNDATTWDEHIIELNLAQPVSLGHIDLKFTVYQQCSNPAAIQVTLLKQNNNGFGYRMKGPHSSYKPNVIDDNIDLSYIANENPVLSEEYLLAHNAEVLAGPIELSSCIDLCDQGGTATLTSPKLFKTRTRNFLLHIKTVSDPSKDGQNKTRGCDWLHEISISVRAVKSHSRISNGRMQRIAMLESNVLLVQLLRIASDPASSSLAKNLSLDILNWICFIRLNRFRSPKSERLKDQINKQTAANIQDLLAQQFECISLAEKHIEQLLRNCVIYSERSTAHKCVKLLIMLTEGATNLPAEVQDHTTLDYSFKAAIANTFPELPRAQCGSVVRWYMMLTCATSTAESHNSISEQAISLLKEIAAEIDKRWDPHCALLCTRFGLYGYPFEPEIFDFDFPNVSRQGPGSSVANVIRSSPTILPVTGLDIKRLSSIDGVDFRTFPYLIRGKSISNQLRGLLEVVQLHFNCVQTSEATRIDNIDSVGGNTGANVIIEDVMMMPKQVVAKEKGEEMLNDLVNDVECLVEEMKVDENEKQEKVDYDAFGLVNGFKDKTLSSLNSVKQTLKEEVKKEKADIMDKLTIAKFLPKLSLLESYMQKKINEHISFDDFGKLDNWDGNTTPPFNLEVEAPIVCSEATSESNMSQQATSAGGNILDDAADSEKITESLNANPCQPIAWHKLITPPPKQMIVIDRMHSGARRFVVLDFGQHILLTDLVIPSCDELTSLNIDIWCFDEETDCTRLVQVNDIQTKTLVLSDIQPAPICRYLKLTIIGRIGMSATKCKVPLGRFFGHPVVLEHDGYGDQLMKYIKHPTHSLQSQIKSLNALYEDVHCRYSLASSKLMDLLAPMRNSELSNVAHLQAFINKQREEELSGLENNNKVVSLYDECMLLQYQINVIRNVVLRLERALSPNSPTTTLLPGQSIPLTEDILRAASRDKLRVLSLSLVEVLLHFSIEYGIKNILPVHQRFNAATAKALFNALVVYGDAQLQLATCSLLVRMCCFQPWWGDFLADTFCSLFSAQNCKAFPQDRIFFLLTYLGRRSIAMGACRSIVIDAVLKTLVKLLAPISPHYKFQSEQPSTSANAADRNAAMGTTSDLQLITWLLLFLSVCLDDNERKDKSAARWDFMSCEGDFTKARPSATPNIKQLRCFKKRIIQQNKYLAQPYADWGKKIYMIQNEHPGIFMELSTKSKGAGPTKSTSTGGKINMGSSGLCVGGPSSSSGNTSKSAASSSSPKNQAVPGTDGERDSNFDKGLKSLRMDNIKVVIRGLFALLLEMDFDCNMDQFLLTCKVIARLVAACRPSVQLCKIVTIAQLQQLVRLSVWNDQQHPWAVHAITCLLQDLLDADKQFRDNDVTPTNDTPRTMEAAIQETRDLFSDYTSVKYNYLPSLIECDDTEFDEMLNDIDMIERTKPVTKKESNALSKNTFNFFCKSISIALDSRLDVGLELHVETQLRRLTNRTSLDLYSSLPQVLTNEFVSPPPEAAPWPEYLTQLWSGTEYNGDNTYVMFKRVFDSILAELHLEDTWVHLEQVLQMWLTLNCELSDKPYTSGITHSDVPKIPFGANAVQGLLLALAWHSDIKLRTWCLGFQCLFLACNSLPIGDDADCTRINEAIVNDENFEKVLLRFFSGYGMSSSIITNRCAGPTICKHLHELLLWLHSKSETSGIPCKRRLKDILLHVVLQLVQPGGSISNQQGPIDAQNQLVRDLLMMPNDKGDLNIALKITESVSFLVYNNISNGERLQCQRGNENSPVGNNFSNLFANVLGSENPNKQNAAICDNSLIINLLKLSSHMVLTEMPRQPSEVTIPEEEELSNQSQTDETKAEQLNTEGHRSKVPCIADWVLRHFPTMKRLFGTLSQCSTNTFTMMSSGCFFSPKSNMILDDPQTIADAVFSLMITLNDKASHPRLVVVPICRYLEETTIPRNATLPRLPHSEPFLWYISKVLEVPAAVQTFTQLGGIQIICHSLVRLNKTIINMQPGLISLIMQHLTRNTRFKRNNHVAASSACKKTTTGTGTTCPTSAQPPRSAAQYDGLINFAPFSHIVSENDATQSAEVLISNPNNSHRRPRSPAWTYMFYPNETHVDLTITLPTAILLKEVQLVPHTTSLASCPSAVALELSRDYGLGSIPVGAPMATTGLTCIRLKLAKAEVATSIVLRLYKPKDCGNVGLVQIAVLGQTIFGNRMHGLRSPSPFIDSLATCSSPVQTAVLAAMDADSMLEDDAFAKTSIGWVRILSRCFHVAALQPDSKLADLIAAYPAAYPGFLEACCSLLNIMPMIPSLAQQHLETILLKLSAYNHEISLQLLRTLLWHTTPQVFKLSSDSVCDLIYEVVTGSTEHLLDKLRVLIDWVMQLHDNYSKQARCNNPQSGFVKVIASILWKVHTQQQLPELPLLISSSLFETCFEWVSSLEHDEPLKSSFDSLLCALCFIKPELFNQLLVKLGVKLEPPARGQTDDSKVQSGSAWFRREGSENLSVLLQRPTFLKTLALACQSPAAVYQMLDSGLPKLLAHAIYEYCMHLMPGSEPVASASTVPEDEPLANNQAAAAADDEDSSLTDFDKAEANVVYNTPLLSSSNVPKVLDFFAECCAEGPMRDWLGTMQGSVFWKPLLQLLCNTHAAQFSKTLPKEQSFIRLERATINFFTRVSACHPGNQENLTSLLIGAIICKPLRFSHGVRPTISGFTRQMVLQLLLENEHITVSVRSQQPLQRRDTLSAVLGGISSGASTSLPVAAVNNHPARRSSAHHMLFTVTTSTTCQEIMQNCVSVFSNLVYQQPTDQRAGETSASSSGVSSATAKSSESKMEKSSFLNFNNVEAGSMEFLTVGAAVAAKDKRIKDAKNQAAMNKDKETQTSSTNLASNIFNVDEFLLQSANAVNGNQLVIPEYSDIVLAGDATISQVLASLQNAGHSLSTPCISFDLVLQQRSDDTSEAKKTKAACTEPLPSPLQRFSSSGGLSLLAHYLPTVYPEHSGRKTTLVFSEKEKSPPLSDWVKLEPNDEIYEDLEDTLCEPAAKLATVSSVPQHSLAAFGLFLRLPAYSDVLLRDKMRAQCLLRLVLGVTGDGDGNDIYSLSLAPSLPTLPFEVFRQLLDSVPLSTDDGVLLRRVVIEVGAMHLVLNCLGIFSHQSHNNRIGAPISEPSPSGTSSGSGTIGNGSGNGSVSGTKTNAPEEATPSATSDDKSHMYWAKGTGFGTGSTTQSWNVEQALLRQKSEEEHVTVLLLVLASYINPGDCIPSEMCGEDILAYHDVRDVTGELPPIFQTLLQQSCLIPALSSYLRNDSVLDITRHIPLYRAILKLLRALSLSKMLVSLLQPSASGSGDSTPPIVELLTSMKTCVDTYAKRLKVNKKSNIKGQTHQLTFNIDDGDDEGLALLIPDIHETSVLVQKTTDADALINMLHTNEDGSAQLEPQSKSIEQRYLELMKKRQFDTFDMIVESDNNSFRFVVSHHFEKMVRLAGDRYHPSRVKRLAQEAVTLSTSLPLSFSSSVFVRCDTDRLDIMKVLITGPADTPYANGCFEFDVFFPPDYPNQPMLINLETTGRHSVRFNPNLYNDGKVCLSVLNTWHGRPEEKWNAQTSSFLQVLVSIQSLILVPEPYFNEPGFERSRGSPSGTNSSREYNSNIYQACVRWAMLEQIRSPSQCFKDVIHKHFWLKREEICAQIEGWIEELGKPQYTERASRTISFNSMVLRRHYRHLREELSKLKPPRGLEDLDAPFNPVATLPPMDVSAVAPSLAATNTVQAQPDDAIVTDITLLGENADECEADGDAEGDGEVDDGFKGFPQEEGLLTGEEEVVEILSDTENESSMWQ

Target ORF information:

RefSeq Version	NM_001275532.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275532.2

ORF Insert Sequence:

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCGTCAAAT ATAATTACCT ACCTTCATTA 
ATTGAATGCG ATGACACGGA ATTCGATGAA ATGCTAAACG ACATTGACAT GATTGAACGA 
ACGAAGCCAG TTACCAAAAA GGAAAGTAAT GCCCTGTCTA AGAATACATT CAATTTCTTC 
TGCAAGTCTA TCTCGATTGC TCTGGATTCA CGATTGGACG TGGGTCTGGA ATTGCACGTA 
GAAACTCAGC TGAGAAGGCT AACAAATCGC ACATCCCTGG ATCTCTATTC ATCGCTTCCT 
CAGGTGTTGA CCAATGAGTT TGTCTCTCCA CCACCTGAGG CTGCTCCTTG GCCAGAATAT 
TTGACTCAAC TTTGGTCAGG CACGGAATAC AACGGTGACA ATACTTATGT GATGTTTAAA 
AGGGTCTTTG ACTCCATCCT GGCGGAATTG CACCTTGAAG ACACGTGGGT GCACTTGGAG 
CAGGTGCTGC AGATGTGGCT GACGCTAAAT TGCGAGCTCT CGGACAAGCC ATACACCAGT 
GGCATCACTC ACTCGGATGT TCCAAAAATT CCGTTTGGAG CAAATGCGGT GCAGGGATTG 
CTCCTGGCTT TGGCTTGGCA CAGTGATATA AAACTGCGGA CCTGGTGTCT TGGCTTTCAG 
TGCCTGTTTC TCGCTTGCAA CTCATTGCCT ATCGGAGATG ATGCGGATTG TACGCGTATC 
AACGAGGCTA TCGTGAATGA TGAAAACTTT GAAAAAGTGC TGCTACGATT CTTTTCCGGT 
TATGGCATGA GCTCATCTAT CATCACCAAC CGATGCGCTG GACCTACTAT TTGCAAGCAC 
TTGCATGAGT TATTGCTCTG GCTGCACAGC AAAAGCGAGA CGAGTGGCAT TCCGTGCAAG 
CGTCGACTTA AGGACATTTT GTTGCATGTT GTGCTTCAGT TGGTGCAGCC TGGTGGCTCT 
ATTAGCAACC AGCAGGGTCC AATTGATGCT CAAAACCAGC TGGTTCGTGA TTTGCTTATG 
ATGCCCAATG ATAAGGGGGA CCTCAACATT GCGCTTAAAA TCACAGAAAG TGTTTCTTTC 
CTGGTGTACA ACAACATATC CAATGGCGAG AGGCTACAAT GTCAGCGTGG AAATGAAAAC 
AGCCCAGTTG GCAACAATTT TAGCAATTTG TTTGCCAACG TTCTGGGATC AGAAAATCCC 
AACAAGCAGA ATGCCGCAAT TTGCGACAAC TCACTTATTA TAAATCTGCT GAAACTATCG 
TCACACATGG TATTGACAGA GATGCCCCGG CAACCAAGTG AGGTTACCAT TCCCGAGGAG 
GAGGAACTTT CCAATCAGAG TCAAACGGAT GAGACTAAAG CGGAGCAGTT AAACACGGAA 
GGACATCGCA GCAAAGTGCC TTGCATTGCA GATTGGGTAC TCCGCCATTT CCCCACAATG 
AAGCGACTTT TTGGAACGCT TTCCCAGTGC TCGACAAACA CCTTCACCAT GATGTCCTCT 
GGCTGTTTCT TTTCACCGAA GTCCAATATG ATTTTAGATG ACCCGCAGAC AATTGCAGAC 
GCCGTCTTTA GTTTGATGAT TACTCTCAAC GACAAGGCTT CGCATCCCCG ACTTGTTGTG 
GTGCCCATTT GCCGATACCT TGAAGAAACC ACTATTCCGA GAAATGCAAC GCTGCCGCGT 
CTGCCGCATT CTGAACCTTT CCTGTGGTAC ATCTCGAAGG TATTGGAAGT ACCAGCGGCA 
GTGCAAACGT TCACCCAATT GGGCGGAATT CAGATTATAT GTCATAGCCT GGTGCGACTT 
AATAAAACTA TCATCAACAT GCAACCAGGA TTGATTTCTT TGATTATGCA ACACTTGACG 
CGCAATACGC GCTTTAAGCG TAATAATCAT GTGGCGGCAA GTAGTGCTTG CAAAAAGACA 
ACGACTGGTA CGGGAACCAC ATGTCCCACG TCAGCACAGC CGCCGAGAAG TGCTGCTCAG 
TACGATGGTC TGATCAACTT TGCACCCTTC TCGCATATTG TCTCCGAAAA TGATGCGACA 
CAAAGTGCAG AGGTACTAAT ATCAAATCCA AATAATTCAC ATCGAAGACC GCGATCGCCA 
GCCTGGACCT ATATGTTCTA TCCAAATGAG ACGCATGTGG ATCTGACTAT AACGTTGCCC 
ACAGCAATTT TGTTGAAAGA GGTGCAGCTG GTGCCGCACA CAACTAGTTT GGCATCCTGT 
CCATCTGCGG TGGCTCTAGA GCTGTCTAGA GATTACGGCC TTGGTTCGAT CCCAGTGGGT 
GCTCCCATGG CCACCACTGG TCTGACCTGC ATTAGACTAA AGCTGGCCAA GGCAGAGGTG 
GCTACCAGCA TTGTTCTGAG GCTGTACAAA CCCAAGGACT GTGGAAATGT GGGTCTCGTA 
CAAATCGCTG TGCTTGGTCA GACAATCTTC GGAAATCGCA TGCATGGCCT TCGCTCACCG 
AGTCCGTTCA TTGACTCCCT AGCAACGTGC TCCTCGCCAG TGCAAACGGC AGTTTTAGCC 
GCAATGGATG CGGATTCCAT GCTTGAAGAT GATGCTTTTG CGAAGACTAG CATTGGCTGG 
GTTCGCATTC TGTCGCGCTG TTTCCATGTG GCCGCTTTAC AACCAGACAG TAAGCTGGCT 
GATCTTATCG CCGCCTATCC GGCTGCATAT CCCGGCTTTT TAGAGGCCTG CTGCTCATTG 
CTAAACATAA TGCCAATGAT TCCTAGTCTT GCACAGCAAC ACTTGGAGAC CATTTTGTTA 
AAATTAAGTG CATACAACCA CGAGATCAGT TTGCAACTTC TTAGGACGCT CCTGTGGCAC 
ACCACTCCGC AAGTTTTTAA ACTCTCCAGC GATTCGGTGT GTGATCTTAT TTATGAAGTA 
GTTACCGGAT CGACGGAGCA CCTACTGGAC AAACTACGCG TGCTTATCGA CTGGGTAATG 
CAATTGCACG ACAACTACAG CAAGCAGGCG CGCTGTAATA ACCCACAAAG TGGATTTGTT 
AAGGTGATTG CTTCGATTCT GTGGAAAGTG CATACCCAGC AACAATTGCC TGAGTTACCG 
CTTCTCATTT CATCTTCGCT GTTCGAGACC TGTTTTGAAT GGGTCTCTTC GCTGGAGCAC 
GATGAGCCAC TGAAGAGCAG TTTTGACTCC TTGTTGTGTG CATTGTGCTT CATTAAGCCA 
GAACTGTTTA ACCAGCTGTT GGTCAAGCTG GGAGTTAAGC TGGAACCTCC AGCTCGAGGT 
CAAACTGATG ACAGCAAAGT GCAAAGTGGT AGTGCTTGGT TCCGCCGTGA GGGTAGCGAA 
AACCTTAGTG TTTTGCTACA GCGTCCCACA TTCCTAAAGA CTCTAGCCTT GGCCTGCCAG 
TCCCCTGCAG CAGTTTACCA GATGCTAGAC TCCGGACTTC CAAAGCTATT GGCCCACGCC 
ATTTACGAGT ATTGCATGCA TCTCATGCCG GGATCGGAGC CAGTAGCTTC TGCATCTACA 
GTGCCGGAAG ACGAACCGTT AGCCAACAAT CAGGCAGCCG CTGCAGCAGA TGACGAGGAC 
TCGTCTCTTA CGGACTTTGA CAAGGCCGAG GCAAATGTGG TTTATAATAC TCCACTGCTA 
AGTAGCTCGA ATGTACCCAA AGTGCTAGAT TTCTTTGCCG AATGTTGTGC CGAAGGTCCA 
ATGCGTGATT GGTTAGGTAC TATGCAGGGC TCCGTATTTT GGAAGCCTCT GCTGCAGTTG 
CTCTGTAACA CACATGCCGC CCAGTTCAGC AAGACGTTGC CTAAGGAACA ATCGTTTATC 
CGCCTGGAGA GGGCCACAAT CAACTTCTTT ACGCGAGTTA GCGCCTGCCA TCCCGGAAAC 
CAGGAAAATC TTACATCGTT GCTCATTGGA GCCATAATTT GCAAACCATT AAGATTTTCG 
CATGGAGTTC GACCAACTAT ATCTGGATTT ACTAGACAGA TGGTGCTGCA GCTCCTCCTC 
GAGAATGAAC ACATCACAGT CTCTGTTAGA AGTCAGCAGC CCTTGCAACG GAGGGATACC 
CTCTCCGCCG TTCTAGGCGG GATTTCGAGT GGTGCAAGTA CTAGCTTACC CGTAGCAGCA 
GTCAACAATC ATCCGGCCAG GAGGAGCAGC GCCCACCACA TGCTATTCAC GGTGACCACT 
AGCACCACGT GCCAAGAGAT TATGCAGAAT TGCGTTAGTG TGTTTAGTAA TCTAGTTTAT 
CAACAACCAA CAGATCAGCG AGCTGGCGAG ACCTCTGCCT CGAGCAGCGG CGTATCGAGT 
GCAACTGCCA AATCCAGTGA GAGCAAAATG GAAAAGAGCA GCTTCCTGAA CTTTAACAAC 
GTAGAAGCGG GTAGCATGGA GTTCCTTACC GTTGGTGCTG CTGTGGCAGC CAAGGACAAG 
CGCATAAAAG ATGCTAAGAA CCAAGCAGCC ATGAACAAGG ACAAGGAGAC TCAGACCAGC 
TCAACGAACT TGGCTTCCAA TATATTCAAT GTGGATGAGT TCTTGTTGCA ATCGGCGAAT 
GCCGTTAATG GTAACCAGTT GGTCATTCCA GAGTATTCTG ATATTGTTCT AGCTGGAGAT 
GCCACCATCT CGCAGGTTTT GGCCTCGCTT CAGAATGCAG GACATTCTCT TTCGACGCCC 
TGTATATCGT TTGATCTGGT ACTTCAACAG CGCTCTGATG ACACAAGCGA GGCAAAGAAG 
ACCAAAGCTG CCTGCACTGA GCCGTTGCCA TCACCACTGC AACGATTTTC CAGCAGTGGT 
GGTCTATCTT TACTCGCTCA CTACCTGCCC ACCGTATATC CGGAACACTC AGGTCGCAAG 
ACCACCTTGG TGTTTAGCGA GAAGGAGAAA AGTCCCCCGC TCTCTGACTG GGTGAAATTG 
GAACCGAACG ACGAGATCTA TGAGGATTTG GAGGACACTC TCTGTGAGCC AGCAGCTAAG 
CTGGCAACGG TCAGTTCGGT GCCGCAGCAC TCGCTGGCCG CATTTGGATT ATTCCTGCGC 
CTGCCCGCCT ACTCTGATGT CTTGCTGCGA GACAAGATGC GGGCGCAGTG TCTTCTCCGC 
TTAGTGCTCG GAGTCACGGG AGATGGGGAT GGCAATGATA TATACAGTCT GTCGCTGGCA 
CCCTCCTTGC CTACATTACC CTTCGAGGTC TTCCGTCAGC TTCTTGATAG TGTGCCGTTA 
TCCACCGACG ACGGTGTTTT GCTACGACGC GTGGTAATCG AAGTGGGGGC CATGCACCTA 
GTGCTTAATT GCCTCGGCAT TTTCTCGCAT CAGTCGCACA ACAACAGGAT TGGAGCACCG 
ATTAGCGAAC CATCGCCAAG TGGAACCAGT AGTGGTAGCG GCACCATTGG CAATGGAAGT 
GGAAACGGAA GTGTAAGCGG TACCAAAACT AATGCACCAG AAGAGGCCAC ACCTAGTGCC 
ACGTCGGACG ACAAAAGCCA CATGTACTGG GCCAAGGGCA CTGGTTTCGG TACCGGTTCT 
ACTACTCAGT CGTGGAATGT GGAGCAGGCG CTTCTTCGGC AAAAGAGCGA GGAGGAGCAT 
GTCACAGTGT TGCTGCTTGT TCTAGCCAGT TACATAAATC CGGGTGACTG TATCCCAAGT 
GAAATGTGCG GAGAGGACAT CCTAGCTTAT CATGATGTCC GAGATGTTAC CGGCGAGTTG 
CCGCCCATCT TCCAAACCCT ACTGCAGCAG TCTTGCTTAA TCCCCGCCCT CAGCTCCTAT 
CTGCGCAACG ATTCAGTGCT TGATATCACG CGCCACATTC CGCTATACCG TGCCATACTC 
AAACTTCTGC GTGCCCTATC GCTGTCTAAG ATGCTTGTCT CGTTGCTCCA ACCATCTGCC 
AGTGGCAGTG GGGACAGCAC GCCACCCATA GTGGAGTTGC TCACAAGCAT GAAGACCTGT 
GTGGATACCT ACGCCAAACG GTTAAAAGTT AACAAGAAGT CAAACATAAA GGGACAGACA 
CATCAGTTGA CCTTTAACAT CGATGATGGC GACGATGAGG GATTAGCTTT GCTTATTCCG 
GATATCCATG AAACCAGTGT GCTGGTGCAA AAAACCACAG ATGCTGATGC GCTAATCAAC 
ATGTTGCATA CCAACGAAGA CGGCAGCGCA CAGCTAGAGC CCCAGAGCAA ATCAATTGAA 
CAGCGCTACT TAGAGCTAAT GAAGAAGCGC CAGTTCGATA CGTTTGACAT GATTGTGGAG 
TCGGATAACA ACAGTTTCCG CTTTGTGGTC TCGCATCACT TCGAAAAAAT GGTTCGGCTC 
GCTGGCGATC GTTACCATCC GTCGCGGGTC AAGCGACTTG CCCAGGAGGC AGTTACACTG 
TCCACCAGCC TACCCCTCAG CTTTAGTAGT TCTGTATTTG TACGCTGTGA TACTGATAGA 
TTGGACATCA TGAAGGTACT CATAACTGGT CCGGCGGACA CGCCTTACGC CAACGGGTGC 
TTTGAGTTTG ATGTCTTCTT TCCGCCGGAC TACCCTAACC AGCCCATGCT CATCAATCTG 
GAGACGACGG GTCGCCATTC GGTGCGCTTT AACCCAAACC TCTATAATGA CGGCAAAGTT 
TGCCTGTCAG TTCTCAACAC CTGGCATGGT CGGCCTGAGG AGAAATGGAA TGCACAGACT 
TCAAGCTTCC TGCAGGTACT CGTTTCCATC CAGTCCCTGA TCCTCGTTCC CGAGCCCTAT 
TTCAATGAAC CGGGCTTTGA AAGAAGTCGT GGCAGTCCAA GTGGTACCAA TTCATCGCGA 
GAATATAATA GCAATATATA TCAGGCCTGT GTGCGGTGGG CTATGTTGGA GCAAATACGG 
AGTCCAAGTC AGTGCTTTAA AGATGTCATA CACAAGCACT TCTGGTTGAA GAGAGAGGAG 
ATTTGCGCCC AGATAGAGGG CTGGATCGAG GAACTCGGTA AGCCGCAGTA CACCGAGAGG 
GCCAGTCGCA CTATATCGTT CAACTCAATG GTCCTGCGCC GCCACTATCG CCACCTGCGT 
GAGGAGTTGT CGAAACTGAA GCCGCCACGT GGCCTCGAGG ACTTGGATGC GCCCTTTAAT 
CCTGTGGCCA CGCTACCACC GATGGATGTG TCCGCCGTGG CTCCTTCACT AGCAGCCACA 
AATACCGTCC AAGCGCAACC GGATGATGCC ATAGTGACCG ATATTACCCT GCTCGGCGAG 
AATGCGGATG AATGCGAAGC TGATGGCGAT GCCGAAGGCG ATGGAGAAGT GGACGATGGC 
TTTAAGGGTT TCCCGCAAGA AGAGGGTTTG CTGACGGGCG AGGAAGAGGT CGTGGAAATT 
TTAAGTGATA CGGAGAACGA GAGCAGTATG TGGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bruce ( NM_001275534.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262463.1 Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant E, mRNA.

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CloneID	OFa08257
Clone ID Related Accession (Same CDS sequence)	NM_001275534.2
Accession Version	NM_001275534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BIR repeat containing ubiquitin-conjugating enzyme, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275534.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCACTGCTT CCTCGTGTAC TGAGGCACTA 
TTTGAGACGC TGATGCCGAC AGCAAGAGAA TTTTATCAAG AGGCCGTCAA ATATAATTAC 
CTACCTTCAT TAATTGAATG CGATGACACG GAATTCGATG AAATGCTAAA CGACATTGAC 
ATGATTGAAC GAACGAAGCC AGTTACCAAA AAGGAAAGTA ATGCCCTGTC TAAGAATACA 
TTCAATTTCT TCTGCAAGTC TATCTCGATT GCTCTGGATT CACGATTGGA CGTGGGTCTG 
GAATTGCACG TAGAAACTCA GCTGAGAAGG CTAACAAATC GCACATCCCT GGATCTCTAT 
TCATCGCTTC CTCAGGTGTT GACCAATGAG TTTGTCTCTC CACCACCTGA GGCTGCTCCT 
TGGCCAGAAT ATTTGACTCA ACTTTGGTCA GGCACGGAAT ACAACGGTGA CAATACTTAT 
GTGATGTTTA AAAGGGTCTT TGACTCCATC CTGGCGGAAT TGCACCTTGA AGACACGTGG 
GTGCACTTGG AGCAGGTGCT GCAGATGTGG CTGACGCTAA ATTGCGAGCT CTCGGACAAG 
CCATACACCA GTGGCATCAC TCACTCGGAT GTTCCAAAAA TTCCGTTTGG AGCAAATGCG 
GTGCAGGGAT TGCTCCTGGC TTTGGCTTGG CACAGTGATA TAAAACTGCG GACCTGGTGT 
CTTGGCTTTC AGTGCCTGTT TCTCGCTTGC AACTCATTGC CTATCGGAGA TGATGCGGAT 
TGTACGCGTA TCAACGAGGC TATCGTGAAT GATGAAAACT TTGAAAAAGT GCTGCTACGA 
TTCTTTTCCG GTTATGGCAT GAGCTCATCT ATCATCACCA ACCGATGCGC TGGACCTACT 
ATTTGCAAGC ACTTGCATGA GTTATTGCTC TGGCTGCACA GCAAAAGCGA GACGAGTGGC 
ATTCCGTGCA AGCGTCGACT TAAGGACATT TTGTTGCATG TTGTGCTTCA GTTGGTGCAG 
CCTGGTGGCT CTATTAGCAA CCAGCAGGGT CCAATTGATG CTCAAAACCA GCTGGTTCGT 
GATTTGCTTA TGATGCCCAA TGATAAGGGG GACCTCAACA TTGCGCTTAA AATCACAGAA 
AGTGTTTCTT TCCTGGTGTA CAACAACATA TCCAATGGCG AGAGGCTACA ATGTCAGCGT 
GGAAATGAAA ACAGCCCAGT TGGCAACAAT TTTAGCAATT TGTTTGCCAA CGTTCTGGGA 
TCAGAAAATC CCAACAAGCA GAATGCCGCA ATTTGCGACA ACTCACTTAT TATAAATCTG 
CTGAAACTAT CGTCACACAT GGTATTGACA GAGATGCCCC GGCAACCAAG TGAGGTTACC 
ATTCCCGAGG AGGAGGAACT TTCCAATCAG AGTCAAACGG ATGAGACTAA AGCGGAGCAG 
TTAAACACGG AAGGACATCG CAGCAAAGTG CCTTGCATTG CAGATTGGGT ACTCCGCCAT 
TTCCCCACAA TGAAGCGACT TTTTGGAACG CTTTCCCAGT GCTCGACAAA CACCTTCACC 
ATGATGTCCT CTGGCTGTTT CTTTTCACCG AAGTCCAATA TGATTTTAGA TGACCCGCAG 
ACAATTGCAG ACGCCGTCTT TAGTTTGATG ATTACTCTCA ACGACAAGGC TTCGCATCCC 
CGACTTGTTG TGGTGCCCAT TTGCCGATAC CTTGAAGAAA CCACTATTCC GAGAAATGCA 
ACGCTGCCGC GTCTGCCGCA TTCTGAACCT TTCCTGTGGT ACATCTCGAA GGTATTGGAA 
GTACCAGCGG CAGTGCAAAC GTTCACCCAA TTGGGCGGAA TTCAGATTAT ATGTCATAGC 
CTGGTGCGAC TTAATAAAAC TATCATCAAC ATGCAACCAG GATTGATTTC TTTGATTATG 
CAACACTTGA CGCGCAATAC GCGCTTTAAG CGTAATAATC ATGTGGCGGC AAGTAGTGCT 
TGCAAAAAGA CAACGACTGG TACGGGAACC ACATGTCCCA CGTCAGCACA GCCGCCGAGA 
AGTGCTGCTC AGTACGATGG TCTGATCAAC TTTGCACCCT TCTCGCATAT TGTCTCCGAA 
AATGATGCGA CACAAAGTGC AGAGGTACTA ATATCAAATC CAAATAATTC ACATCGAAGA 
CCGCGATCGC CAGCCTGGAC CTATATGTTC TATCCAAATG AGACGCATGT GGATCTGACT 
ATAACGTTGC CCACAGCAAT TTTGTTGAAA GAGGTGCAGC TGGTGCCGCA CACAACTAGT 
TTGGCATCCT GTCCATCTGC GGTGGCTCTA GAGCTGTCTA GAGATTACGG CCTTGGTTCG 
ATCCCAGTGG GTGCTCCCAT GGCCACCACT GGTCTGACCT GCATTAGACT AAAGCTGGCC 
AAGGCAGAGG TGGCTACCAG CATTGTTCTG AGGCTGTACA AACCCAAGGA CTGTGGAAAT 
GTGGGTCTCG TACAAATCGC TGTGCTTGGT CAGACAATCT TCGGAAATCG CATGCATGGC 
CTTCGCTCAC CGAGTCCGTT CATTGACTCC CTAGCAACGT GCTCCTCGCC AGTGCAAACG 
GCAGTTTTAG CCGCAATGGA TGCGGATTCC ATGCTTGAAG ATGATGCTTT TGCGAAGACT 
AGCATTGGCT GGGTTCGCAT TCTGTCGCGC TGTTTCCATG TGGCCGCTTT ACAACCAGAC 
AGTAAGCTGG CTGATCTTAT CGCCGCCTAT CCGGCTGCAT ATCCCGGCTT TTTAGAGGCC 
TGCTGCTCAT TGCTAAACAT AATGCCAATG ATTCCTAGTC TTGCACAGCA ACACTTGGAG 
ACCATTTTGT TAAAATTAAG TGCATACAAC CACGAGATCA GTTTGCAACT TCTTAGGACG 
CTCCTGTGGC ACACCACTCC GCAAGTTTTT AAACTCTCCA GCGATTCGGT GTGTGATCTT 
ATTTATGAAG TAGTTACCGG ATCGACGGAG CACCTACTGG ACAAACTACG CGTGCTTATC 
GACTGGGTAA TGCAATTGCA CGACAACTAC AGCAAGCAGG CGCGCTGTAA TAACCCACAA 
AGTGGATTTG TTAAGGTGAT TGCTTCGATT CTGTGGAAAG TGCATACCCA GCAACAATTG 
CCTGAGTTAC CGCTTCTCAT TTCATCTTCG CTGTTCGAGA CCTGTTTTGA ATGGGTCTCT 
TCGCTGGAGC ACGATGAGCC ACTGAAGAGC AGTTTTGACT CCTTGTTGTG TGCATTGTGC 
TTCATTAAGC CAGAACTGTT TAACCAGCTG TTGGTCAAGC TGGGAGTTAA GCTGGAACCT 
CCAGCTCGAG GTCAAACTGA TGACAGCAAA GTGCAAAGTG GTAGTGCTTG GTTCCGCCGT 
GAGGGTAGCG AAAACCTTAG TGTTTTGCTA CAGCGTCCCA CATTCCTAAA GACTCTAGCC 
TTGGCCTGCC AGTCCCCTGC AGCAGTTTAC CAGATGCTAG ACTCCGGACT TCCAAAGCTA 
TTGGCCCACG CCATTTACGA GTATTGCATG CATCTCATGC CGGGATCGGA GCCAGTAGCT 
TCTGCATCTA CAGTGCCGGA AGACGAACCG TTAGCCAACA ATCAGGCAGC CGCTGCAGCA 
GATGACGAGG ACTCGTCTCT TACGGACTTT GACAAGGCCG AGGCAAATGT GGTTTATAAT 
ACTCCACTGC TAAGTAGCTC GAATGTACCC AAAGTGCTAG ATTTCTTTGC CGAATGTTGT 
GCCGAAGGTC CAATGCGTGA TTGGTTAGGT ACTATGCAGG GCTCCGTATT TTGGAAGCCT 
CTGCTGCAGT TGCTCTGTAA CACACATGCC GCCCAGTTCA GCAAGACGTT GCCTAAGGAA 
CAATCGTTTA TCCGCCTGGA GAGGGCCACA ATCAACTTCT TTACGCGAGT TAGCGCCTGC 
CATCCCGGAA ACCAGGAAAA TCTTACATCG TTGCTCATTG GAGCCATAAT TTGCAAACCA 
TTAAGATTTT CGCATGGAGT TCGACCAACT ATATCTGGAT TTACTAGACA GATGGTGCTG 
CAGCTCCTCC TCGAGAATGA ACACATCACA GTCTCTGTTA GAAGTCAGCA GCCCTTGCAA 
CGGAGGGATA CCCTCTCCGC CGTTCTAGGC GGGATTTCGA GTGGTGCAAG TACTAGCTTA 
CCCGTAGCAG CAGTCAACAA TCATCCGGCC AGGAGGAGCA GCGCCCACCA CATGCTATTC 
ACGGTGACCA CTAGCACCAC GTGCCAAGAG ATTATGCAGA ATTGCGTTAG TGATCAGCGA 
GCTGGCGAGA CCTCTGCCTC GAGCAGCGGC GTATCGAGTG CAACTGCCAA ATCCAGTGAG 
AGCAAAATGG AAAAGAGCAG CTTCCTGAAC TTTAACAACG TAGAAGCGGG TAGCATGGAG 
TTCCTTACCG TTGGTGCTGC TGTGGCAGCC AAGGACAAGC GCATAAAAGA TGCTAAGAAC 
CAAGCAGCCA TGAACAAGGA CAAGGAGACT CAGACCAGCT CAACGAACTT GGCTTCCAAT 
ATATTCAATG TGGATGAGTT CTTGTTGCAA TCGGCGAATG CCGTTAATGG TAACCAGTTG 
GTCATTCCAG AGTATTCTGA TATTGTTCTA GCTGGAGATG CCACCATCTC GCAGGTTTTG 
GCCTCGCTTC AGAATGCAGG ACATTCTCTT TCGACGCCCT GTATATCGTT TGATCTGGTA 
CTTCAACAGC GCTCTGATGA CACAAGCGAG GCAAAGAAGA CCAAAGCTGC CTGCACTGAG 
CCGTTGCCAT CACCACTGCA ACGATTTTCC AGCAGTGGTG GTCTATCTTT ACTCGCTCAC 
TACCTGCCCA CCGTATATCC GGAACACTCA GGTCGCAAGA CCACCTTGGT GTTTAGCGAG 
AAGGAGAAAA GTCCCCCGCT CTCTGACTGG GTGAAATTGG AACCGAACGA CGAGATCTAT 
GAGGATTTGG AGGACACTCT CTGTGAGCCA GCAGCTAAGC TGGCAACGGT CAGTTCGGTG 
CCGCAGCACT CGCTGGCCGC ATTTGGATTA TTCCTGCGCC TGCCCGCCTA CTCTGATGTC 
TTGCTGCGAG ACAAGATGCG GGCGCAGTGT CTTCTCCGCT TAGTGCTCGG AGTCACGGGA 
GATGGGGATG GCAATGATAT ATACAGTCTG TCGCTGGCAC CCTCCTTGCC TACATTACCC 
TTCGAGGTCT TCCGTCAGCT TCTTGATAGT GTGCCGTTAT CCACCGACGA CGGTGTTTTG 
CTACGACGCG TGGTAATCGA AGTGGGGGCC ATGCACCTAG TGCTTAATTG CCTCGGCATT 
TTCTCGCATC AGTCGCACAA CAACAGGATT GGAGCACCGA TTAGCGAACC ATCGCCAAGT 
GGAACCAGTA GTGGTAGCGG CACCATTGGC AATGGAAGTG GAAACGGAAG TGTAAGCGGT 
ACCAAAACTA ATGCACCAGA AGAGGCCACA CCTAGTGCCA CGTCGGACGA CAAAAGCCAC 
ATGTACTGGG CCAAGGGCAC TGGTTTCGGT ACCGGTTCTA CTACTCAGTC GTGGAATGTG 
GAGCAGGCGC TTCTTCGGCA AAAGAGCGAG GAGGAGCATG TCACAGTGTT GCTGCTTGTT 
CTAGCCAGTT ACATAAATCC GGGTGACTGT ATCCCAAGTG AAATGTGCGG AGAGGACATC 
CTAGCTTATC ATGATGTCCG AGATGTTACC GGCGAGTTGC CGCCCATCTT CCAAACCCTA 
CTGCAGCAGT CTTGCTTAAT CCCCGCCCTC AGCTCCTATC TGCGCAACGA TTCAGTGCTT 
GATATCACGC GCCACATTCC GCTATACCGT GCCATACTCA AACTTCTGCG TGCCCTATCG 
CTGTCTAAGA TGCTTGTCTC GTTGCTCCAA CCATCTGCCA GTGGCAGTGG GGACAGCACG 
CCACCCATAG TGGAGTTGCT CACAAGCATG AAGACCTGTG TGGATACCTA CGCCAAACGG 
TTAAAAGTTA ACAAGAAGTC AAACATAAAG GGACAGACAC ATCAGTTGAC CTTTAACATC 
GATGATGGCG ACGATGAGGG ATTAGCTTTG CTTATTCCGG ATATCCATGA AACCAGTGTG 
CTGGTGCAAA AAACCACAGA TGCTGATGCG CTAATCAACA TGTTGCATAC CAACGAAGAC 
GGCAGCGCAC AGCTAGAGCC CCAGAGCAAA TCAATTGAAC AGCGCTACTT AGAGCTAATG 
AAGAAGCGCC AGTTCGATAC GTTTGACATG ATTGTGGAGT CGGATAACAA CAGTTTCCGC 
TTTGTGGTCT CGCATCACTT CGAAAAAATG GTTCGGCTCG CTGGCGATCG TTACCATCCG 
TCGCGGGTCA AGCGACTTGC CCAGGAGGCA GTTACACTGT CCACCAGCCT ACCCCTCAGC 
TTTAGTAGTT CTGTATTTGT ACGCTGTGAT ACTGATAGAT TGGACATCAT GAAGGTACTC 
ATAACTGGTC CGGCGGACAC GCCTTACGCC AACGGGTGCT TTGAGTTTGA TGTCTTCTTT 
CCGCCGGACT ACCCTAACCA GCCCATGCTC ATCAATCTGG AGACGACGGG TCGCCATTCG 
GTGCGCTTTA ACCCAAACCT CTATAATGAC GGCAAAGTTT GCCTGTCAGT TCTCAACACC 
TGGCATGGTC GGCCTGAGGA GAAATGGAAT GCACAGACTT CAAGCTTCCT GCAGGTACTC 
GTTTCCATCC AGTCCCTGAT CCTCGTTCCC GAGCCCTATT TCAATGAACC GGGCTTTGAA 
AGAAGTCGTG GCAGTCCAAG TGGTACCAAT TCATCGCGAG AATATAATAG CAATATATAT 
CAGGCCTGTG TGCGGTGGGC TATGTTGGAG CAAATACGGA GTCCAAGTCA GTGCTTTAAA 
GATGTCATAC ACAAGCACTT CTGGTTGAAG AGAGAGGAGA TTTGCGCCCA GATAGAGGGC 
TGGATCGAGG AACTCGGTAA GCCGCAGTAC ACCGAGAGGG CCAGTCGCAC TATATCGTTC 
AACTCAATGG TCCTGCGCCG CCACTATCGC CACCTGCGTG AGGAGTTGTC GAAACTGAAG 
CCGCCACGTG GCCTCGAGGA CTTGGATGCG CCCTTTAATC CTGTGGCCAC GCTACCACCG 
ATGGATGTGT CCGCCGTGGC TCCTTCACTA GCAGCCACAA ATACCGTCCA AGCGCAACCG 
GATGATGCCA TAGTGACCGA TATTACCCTG CTCGGCGAGA ATGCGGATGA ATGCGAAGCT 
GATGGCGATG CCGAAGGCGA TGGAGAAGTG GACGATGGCT TTAAGGGTTT CCCGCAAGAA 
GAGGGTTTGC TGACGGGCGA GGAAGAGGTC GTGGAAATTT TAAGTGATAC GGAGAACGAG 
AGCAGTATGT GGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262463.1
CDS	167..14764
Translation	MATEQHHQQRVLKQDGYLDLGDCNSAPSRPFEMRNVVYHPQLNVLLLFDNGSTVKVLDANSGVILQTYHLSSNGDSIVHGRYMPLQDKILFWDGHNLGLRGDYNGVLLLDTILQAPIGQNDDYIKLEIILSEAIIFQNCITELESEGLECPCDISNELTQKINQAQLKAKKGIKAQRWNTICLEVPYSSLKLVSNNMVILLKRLERHIPVLAIASAINERLTDMMMGSRVPDFGWNFSNFQRVLMHSEAVRRQTFEKWPHMDYKWALPDQMAQAGFYHQPSSSGEDRAMCFTCSVCLVCWEKTDEPWSEHERHSPLCPFVKGEYTQNVPLSITYATNPALPAPGLGFDIISNSDYANVLCTSCSQTGELSVWSIERHLKLMHTFHVPTLLNYIFEESFEWARVTAICVLPNARARTKVNYVYSAANYGGSGSSNGAGCNSGLNAVSGGKFGSVNAQHITSTSTLRAGVVGSKIVLGVSVRQSSGELALRMVVLNIVEVDRNSESSTSVSNDSGSVSSGGGQLMKSSSPMVNVSAENNATVTQVNLESKDDDNKAMTPYEKFADGFDSNGFVSALMTYNKNNGVSQNTDDASALYDFDLCTLSQILDHNLEEPLSLMVGSSNTVNVSNIGMGNEINKLVVDVEISHQNNNNNNNNNNEASSLGGNSSNNNNNIMINGGTNSSIDKMTAIEEIDCVVESWTSVKRMNALDGASSGVNGVNHMDQEVIEIAEILPTTPKCNQLLVKLLRTKAPEAEGSKIIMATVAKEEEEEDEEQRTDESMETDNNAEGNVAAQLLVFDYDDTEISNDYTLAMTFSRAKFPKQLCILPHFNSSPEHKDAGSICVVCADGTVEIYSLADFQGTMVIEEEGEHFESVVYCRNLDRLCCCSRQGGLLFYSLSDGENDSGDELLEMDDDCSTTLTQAADVSVTDGGRQLARTDLLGKTLLANESICMDAGSEAAAIPNSSSAAQGAHVQTSPSASSAPSTNANLLAYKTADLTLEDLKVFYALTQFDDKLTVYSAEVPSCWNDIGQVQNQRKQSQNMRHGGDERDFTRTWRLHNDATTWDEHIIELNLAQPVSLGHIDLKFTVYQQCSNPAAIQVTLLKQNNNGFGYRMKGPHSSYKPNVIDDNIDLSYIANENPVLSEEYLLAHNAEVLAGPIELSSCIDLCDQGGTATLTSPKLFKTRTRNFLLHIKTVSDPSKDGQNKTRGCDWLHEISISVRAVKSHSRISNGRMQRIAMLESNVLLVQLLRIASDPASSSLAKNLSLDILNWICFIRLNRFRSPKSERLKDQINKQTAANIQDLLAQQFECISLAEKHIEQLLRNCVIYSERSTAHKCVKLLIMLTEGATNLPAEVQDHTTLDYSFKAAIANTFPELPRAQCGSVVRWYMMLTCATSTAESHNSISEQAISLLKEIAAEIDKRWDPHCALLCTRFGLYGYPFEPEIFDFDFPNVSRQGPGSSVANVIRSSPTILPVTGLDIKRLSSIDGVDFRTFPYLIRGKSISNQLRGLLEVVQLHFNCVQTSEATRIDNIDSVGGNTGANVIIEDVMMMPKQVVAKEKGEEMLNDLVNDVECLVEEMKVDENEKQEKVDYDAFGLVNGFKDKTLSSLNSVKQTLKEEVKKEKADIMDKLTIAKFLPKLSLLESYMQKKINEHISFDDFGKLDNWDGNTTPPFNLEVEAPIVCSEATSESNMSQQATSAGGNILDDAADSEKITESLNANPCQPIAWHKLITPPPKQMIVIDRMHSGARRFVVLDFGQHILLTDLVIPSCDELTSLNIDIWCFDEETDCTRLVQVNDIQTKTLVLSDIQPAPICRYLKLTIIGRIGMSATKCKVPLGRFFGHPVVLEHDGYGDQLMKYIKHPTHSLQSQIKSLNALYEDVHCRYSLASSKLMDLLAPMRNSELSNVAHLQAFINKQREEELSGLENNNKVVSLYDECMLLQYQINVIRNVVLRLERALSPNSPTTTLLPGQSIPLTEDILRAASRDKLRVLSLSLVEVLLHFSIEYGIKNILPVHQRFNAATAKALFNALVVYGDAQLQLATCSLLVRMCCFQPWWGDFLADTFCSLFSAQNCKAFPQDRIFFLLTYLGRRSIAMGACRSIVIDAVLKTLVKLLAPISPHYKFQSEQPSTSANAADRNAAMGTTSDLQLITWLLLFLSVCLDDNERKDKSAARWDFMSCEGDFTKARPSATPNIKQLRCFKKRIIQQNKYLAQPYADWGKKIYMIQNEHPGIFMELSTKSKGAGPTKSTSTGGKINMGSSGLCVGGPSSSSGNTSKSAASSSSPKNQAVPGTDGERDSNFDKGLKSLRMDNIKVVIRGLFALLLEMDFDCNMDQFLLTCKVIARLVAACRPSVQLCKIVTIAQLQQLVRLSVWNDQQHPWAVHAITCLLQDLLDADKQFRDNDVTPTNDTPRTMEAAIQETRDLFSDYTSTASSCTEALFETLMPTAREFYQEAVKYNYLPSLIECDDTEFDEMLNDIDMIERTKPVTKKESNALSKNTFNFFCKSISIALDSRLDVGLELHVETQLRRLTNRTSLDLYSSLPQVLTNEFVSPPPEAAPWPEYLTQLWSGTEYNGDNTYVMFKRVFDSILAELHLEDTWVHLEQVLQMWLTLNCELSDKPYTSGITHSDVPKIPFGANAVQGLLLALAWHSDIKLRTWCLGFQCLFLACNSLPIGDDADCTRINEAIVNDENFEKVLLRFFSGYGMSSSIITNRCAGPTICKHLHELLLWLHSKSETSGIPCKRRLKDILLHVVLQLVQPGGSISNQQGPIDAQNQLVRDLLMMPNDKGDLNIALKITESVSFLVYNNISNGERLQCQRGNENSPVGNNFSNLFANVLGSENPNKQNAAICDNSLIINLLKLSSHMVLTEMPRQPSEVTIPEEEELSNQSQTDETKAEQLNTEGHRSKVPCIADWVLRHFPTMKRLFGTLSQCSTNTFTMMSSGCFFSPKSNMILDDPQTIADAVFSLMITLNDKASHPRLVVVPICRYLEETTIPRNATLPRLPHSEPFLWYISKVLEVPAAVQTFTQLGGIQIICHSLVRLNKTIINMQPGLISLIMQHLTRNTRFKRNNHVAASSACKKTTTGTGTTCPTSAQPPRSAAQYDGLINFAPFSHIVSENDATQSAEVLISNPNNSHRRPRSPAWTYMFYPNETHVDLTITLPTAILLKEVQLVPHTTSLASCPSAVALELSRDYGLGSIPVGAPMATTGLTCIRLKLAKAEVATSIVLRLYKPKDCGNVGLVQIAVLGQTIFGNRMHGLRSPSPFIDSLATCSSPVQTAVLAAMDADSMLEDDAFAKTSIGWVRILSRCFHVAALQPDSKLADLIAAYPAAYPGFLEACCSLLNIMPMIPSLAQQHLETILLKLSAYNHEISLQLLRTLLWHTTPQVFKLSSDSVCDLIYEVVTGSTEHLLDKLRVLIDWVMQLHDNYSKQARCNNPQSGFVKVIASILWKVHTQQQLPELPLLISSSLFETCFEWVSSLEHDEPLKSSFDSLLCALCFIKPELFNQLLVKLGVKLEPPARGQTDDSKVQSGSAWFRREGSENLSVLLQRPTFLKTLALACQSPAAVYQMLDSGLPKLLAHAIYEYCMHLMPGSEPVASASTVPEDEPLANNQAAAAADDEDSSLTDFDKAEANVVYNTPLLSSSNVPKVLDFFAECCAEGPMRDWLGTMQGSVFWKPLLQLLCNTHAAQFSKTLPKEQSFIRLERATINFFTRVSACHPGNQENLTSLLIGAIICKPLRFSHGVRPTISGFTRQMVLQLLLENEHITVSVRSQQPLQRRDTLSAVLGGISSGASTSLPVAAVNNHPARRSSAHHMLFTVTTSTTCQEIMQNCVSDQRAGETSASSSGVSSATAKSSESKMEKSSFLNFNNVEAGSMEFLTVGAAVAAKDKRIKDAKNQAAMNKDKETQTSSTNLASNIFNVDEFLLQSANAVNGNQLVIPEYSDIVLAGDATISQVLASLQNAGHSLSTPCISFDLVLQQRSDDTSEAKKTKAACTEPLPSPLQRFSSSGGLSLLAHYLPTVYPEHSGRKTTLVFSEKEKSPPLSDWVKLEPNDEIYEDLEDTLCEPAAKLATVSSVPQHSLAAFGLFLRLPAYSDVLLRDKMRAQCLLRLVLGVTGDGDGNDIYSLSLAPSLPTLPFEVFRQLLDSVPLSTDDGVLLRRVVIEVGAMHLVLNCLGIFSHQSHNNRIGAPISEPSPSGTSSGSGTIGNGSGNGSVSGTKTNAPEEATPSATSDDKSHMYWAKGTGFGTGSTTQSWNVEQALLRQKSEEEHVTVLLLVLASYINPGDCIPSEMCGEDILAYHDVRDVTGELPPIFQTLLQQSCLIPALSSYLRNDSVLDITRHIPLYRAILKLLRALSLSKMLVSLLQPSASGSGDSTPPIVELLTSMKTCVDTYAKRLKVNKKSNIKGQTHQLTFNIDDGDDEGLALLIPDIHETSVLVQKTTDADALINMLHTNEDGSAQLEPQSKSIEQRYLELMKKRQFDTFDMIVESDNNSFRFVVSHHFEKMVRLAGDRYHPSRVKRLAQEAVTLSTSLPLSFSSSVFVRCDTDRLDIMKVLITGPADTPYANGCFEFDVFFPPDYPNQPMLINLETTGRHSVRFNPNLYNDGKVCLSVLNTWHGRPEEKWNAQTSSFLQVLVSIQSLILVPEPYFNEPGFERSRGSPSGTNSSREYNSNIYQACVRWAMLEQIRSPSQCFKDVIHKHFWLKREEICAQIEGWIEELGKPQYTERASRTISFNSMVLRRHYRHLREELSKLKPPRGLEDLDAPFNPVATLPPMDVSAVAPSLAATNTVQAQPDDAIVTDITLLGENADECEADGDAEGDGEVDDGFKGFPQEEGLLTGEEEVVEILSDTENESSMWQ

Target ORF information:

RefSeq Version	NM_001275534.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275534.2

ORF Insert Sequence:

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCACTGCTT CCTCGTGTAC TGAGGCACTA 
TTTGAGACGC TGATGCCGAC AGCAAGAGAA TTTTATCAAG AGGCCGTCAA ATATAATTAC 
CTACCTTCAT TAATTGAATG CGATGACACG GAATTCGATG AAATGCTAAA CGACATTGAC 
ATGATTGAAC GAACGAAGCC AGTTACCAAA AAGGAAAGTA ATGCCCTGTC TAAGAATACA 
TTCAATTTCT TCTGCAAGTC TATCTCGATT GCTCTGGATT CACGATTGGA CGTGGGTCTG 
GAATTGCACG TAGAAACTCA GCTGAGAAGG CTAACAAATC GCACATCCCT GGATCTCTAT 
TCATCGCTTC CTCAGGTGTT GACCAATGAG TTTGTCTCTC CACCACCTGA GGCTGCTCCT 
TGGCCAGAAT ATTTGACTCA ACTTTGGTCA GGCACGGAAT ACAACGGTGA CAATACTTAT 
GTGATGTTTA AAAGGGTCTT TGACTCCATC CTGGCGGAAT TGCACCTTGA AGACACGTGG 
GTGCACTTGG AGCAGGTGCT GCAGATGTGG CTGACGCTAA ATTGCGAGCT CTCGGACAAG 
CCATACACCA GTGGCATCAC TCACTCGGAT GTTCCAAAAA TTCCGTTTGG AGCAAATGCG 
GTGCAGGGAT TGCTCCTGGC TTTGGCTTGG CACAGTGATA TAAAACTGCG GACCTGGTGT 
CTTGGCTTTC AGTGCCTGTT TCTCGCTTGC AACTCATTGC CTATCGGAGA TGATGCGGAT 
TGTACGCGTA TCAACGAGGC TATCGTGAAT GATGAAAACT TTGAAAAAGT GCTGCTACGA 
TTCTTTTCCG GTTATGGCAT GAGCTCATCT ATCATCACCA ACCGATGCGC TGGACCTACT 
ATTTGCAAGC ACTTGCATGA GTTATTGCTC TGGCTGCACA GCAAAAGCGA GACGAGTGGC 
ATTCCGTGCA AGCGTCGACT TAAGGACATT TTGTTGCATG TTGTGCTTCA GTTGGTGCAG 
CCTGGTGGCT CTATTAGCAA CCAGCAGGGT CCAATTGATG CTCAAAACCA GCTGGTTCGT 
GATTTGCTTA TGATGCCCAA TGATAAGGGG GACCTCAACA TTGCGCTTAA AATCACAGAA 
AGTGTTTCTT TCCTGGTGTA CAACAACATA TCCAATGGCG AGAGGCTACA ATGTCAGCGT 
GGAAATGAAA ACAGCCCAGT TGGCAACAAT TTTAGCAATT TGTTTGCCAA CGTTCTGGGA 
TCAGAAAATC CCAACAAGCA GAATGCCGCA ATTTGCGACA ACTCACTTAT TATAAATCTG 
CTGAAACTAT CGTCACACAT GGTATTGACA GAGATGCCCC GGCAACCAAG TGAGGTTACC 
ATTCCCGAGG AGGAGGAACT TTCCAATCAG AGTCAAACGG ATGAGACTAA AGCGGAGCAG 
TTAAACACGG AAGGACATCG CAGCAAAGTG CCTTGCATTG CAGATTGGGT ACTCCGCCAT 
TTCCCCACAA TGAAGCGACT TTTTGGAACG CTTTCCCAGT GCTCGACAAA CACCTTCACC 
ATGATGTCCT CTGGCTGTTT CTTTTCACCG AAGTCCAATA TGATTTTAGA TGACCCGCAG 
ACAATTGCAG ACGCCGTCTT TAGTTTGATG ATTACTCTCA ACGACAAGGC TTCGCATCCC 
CGACTTGTTG TGGTGCCCAT TTGCCGATAC CTTGAAGAAA CCACTATTCC GAGAAATGCA 
ACGCTGCCGC GTCTGCCGCA TTCTGAACCT TTCCTGTGGT ACATCTCGAA GGTATTGGAA 
GTACCAGCGG CAGTGCAAAC GTTCACCCAA TTGGGCGGAA TTCAGATTAT ATGTCATAGC 
CTGGTGCGAC TTAATAAAAC TATCATCAAC ATGCAACCAG GATTGATTTC TTTGATTATG 
CAACACTTGA CGCGCAATAC GCGCTTTAAG CGTAATAATC ATGTGGCGGC AAGTAGTGCT 
TGCAAAAAGA CAACGACTGG TACGGGAACC ACATGTCCCA CGTCAGCACA GCCGCCGAGA 
AGTGCTGCTC AGTACGATGG TCTGATCAAC TTTGCACCCT TCTCGCATAT TGTCTCCGAA 
AATGATGCGA CACAAAGTGC AGAGGTACTA ATATCAAATC CAAATAATTC ACATCGAAGA 
CCGCGATCGC CAGCCTGGAC CTATATGTTC TATCCAAATG AGACGCATGT GGATCTGACT 
ATAACGTTGC CCACAGCAAT TTTGTTGAAA GAGGTGCAGC TGGTGCCGCA CACAACTAGT 
TTGGCATCCT GTCCATCTGC GGTGGCTCTA GAGCTGTCTA GAGATTACGG CCTTGGTTCG 
ATCCCAGTGG GTGCTCCCAT GGCCACCACT GGTCTGACCT GCATTAGACT AAAGCTGGCC 
AAGGCAGAGG TGGCTACCAG CATTGTTCTG AGGCTGTACA AACCCAAGGA CTGTGGAAAT 
GTGGGTCTCG TACAAATCGC TGTGCTTGGT CAGACAATCT TCGGAAATCG CATGCATGGC 
CTTCGCTCAC CGAGTCCGTT CATTGACTCC CTAGCAACGT GCTCCTCGCC AGTGCAAACG 
GCAGTTTTAG CCGCAATGGA TGCGGATTCC ATGCTTGAAG ATGATGCTTT TGCGAAGACT 
AGCATTGGCT GGGTTCGCAT TCTGTCGCGC TGTTTCCATG TGGCCGCTTT ACAACCAGAC 
AGTAAGCTGG CTGATCTTAT CGCCGCCTAT CCGGCTGCAT ATCCCGGCTT TTTAGAGGCC 
TGCTGCTCAT TGCTAAACAT AATGCCAATG ATTCCTAGTC TTGCACAGCA ACACTTGGAG 
ACCATTTTGT TAAAATTAAG TGCATACAAC CACGAGATCA GTTTGCAACT TCTTAGGACG 
CTCCTGTGGC ACACCACTCC GCAAGTTTTT AAACTCTCCA GCGATTCGGT GTGTGATCTT 
ATTTATGAAG TAGTTACCGG ATCGACGGAG CACCTACTGG ACAAACTACG CGTGCTTATC 
GACTGGGTAA TGCAATTGCA CGACAACTAC AGCAAGCAGG CGCGCTGTAA TAACCCACAA 
AGTGGATTTG TTAAGGTGAT TGCTTCGATT CTGTGGAAAG TGCATACCCA GCAACAATTG 
CCTGAGTTAC CGCTTCTCAT TTCATCTTCG CTGTTCGAGA CCTGTTTTGA ATGGGTCTCT 
TCGCTGGAGC ACGATGAGCC ACTGAAGAGC AGTTTTGACT CCTTGTTGTG TGCATTGTGC 
TTCATTAAGC CAGAACTGTT TAACCAGCTG TTGGTCAAGC TGGGAGTTAA GCTGGAACCT 
CCAGCTCGAG GTCAAACTGA TGACAGCAAA GTGCAAAGTG GTAGTGCTTG GTTCCGCCGT 
GAGGGTAGCG AAAACCTTAG TGTTTTGCTA CAGCGTCCCA CATTCCTAAA GACTCTAGCC 
TTGGCCTGCC AGTCCCCTGC AGCAGTTTAC CAGATGCTAG ACTCCGGACT TCCAAAGCTA 
TTGGCCCACG CCATTTACGA GTATTGCATG CATCTCATGC CGGGATCGGA GCCAGTAGCT 
TCTGCATCTA CAGTGCCGGA AGACGAACCG TTAGCCAACA ATCAGGCAGC CGCTGCAGCA 
GATGACGAGG ACTCGTCTCT TACGGACTTT GACAAGGCCG AGGCAAATGT GGTTTATAAT 
ACTCCACTGC TAAGTAGCTC GAATGTACCC AAAGTGCTAG ATTTCTTTGC CGAATGTTGT 
GCCGAAGGTC CAATGCGTGA TTGGTTAGGT ACTATGCAGG GCTCCGTATT TTGGAAGCCT 
CTGCTGCAGT TGCTCTGTAA CACACATGCC GCCCAGTTCA GCAAGACGTT GCCTAAGGAA 
CAATCGTTTA TCCGCCTGGA GAGGGCCACA ATCAACTTCT TTACGCGAGT TAGCGCCTGC 
CATCCCGGAA ACCAGGAAAA TCTTACATCG TTGCTCATTG GAGCCATAAT TTGCAAACCA 
TTAAGATTTT CGCATGGAGT TCGACCAACT ATATCTGGAT TTACTAGACA GATGGTGCTG 
CAGCTCCTCC TCGAGAATGA ACACATCACA GTCTCTGTTA GAAGTCAGCA GCCCTTGCAA 
CGGAGGGATA CCCTCTCCGC CGTTCTAGGC GGGATTTCGA GTGGTGCAAG TACTAGCTTA 
CCCGTAGCAG CAGTCAACAA TCATCCGGCC AGGAGGAGCA GCGCCCACCA CATGCTATTC 
ACGGTGACCA CTAGCACCAC GTGCCAAGAG ATTATGCAGA ATTGCGTTAG TGATCAGCGA 
GCTGGCGAGA CCTCTGCCTC GAGCAGCGGC GTATCGAGTG CAACTGCCAA ATCCAGTGAG 
AGCAAAATGG AAAAGAGCAG CTTCCTGAAC TTTAACAACG TAGAAGCGGG TAGCATGGAG 
TTCCTTACCG TTGGTGCTGC TGTGGCAGCC AAGGACAAGC GCATAAAAGA TGCTAAGAAC 
CAAGCAGCCA TGAACAAGGA CAAGGAGACT CAGACCAGCT CAACGAACTT GGCTTCCAAT 
ATATTCAATG TGGATGAGTT CTTGTTGCAA TCGGCGAATG CCGTTAATGG TAACCAGTTG 
GTCATTCCAG AGTATTCTGA TATTGTTCTA GCTGGAGATG CCACCATCTC GCAGGTTTTG 
GCCTCGCTTC AGAATGCAGG ACATTCTCTT TCGACGCCCT GTATATCGTT TGATCTGGTA 
CTTCAACAGC GCTCTGATGA CACAAGCGAG GCAAAGAAGA CCAAAGCTGC CTGCACTGAG 
CCGTTGCCAT CACCACTGCA ACGATTTTCC AGCAGTGGTG GTCTATCTTT ACTCGCTCAC 
TACCTGCCCA CCGTATATCC GGAACACTCA GGTCGCAAGA CCACCTTGGT GTTTAGCGAG 
AAGGAGAAAA GTCCCCCGCT CTCTGACTGG GTGAAATTGG AACCGAACGA CGAGATCTAT 
GAGGATTTGG AGGACACTCT CTGTGAGCCA GCAGCTAAGC TGGCAACGGT CAGTTCGGTG 
CCGCAGCACT CGCTGGCCGC ATTTGGATTA TTCCTGCGCC TGCCCGCCTA CTCTGATGTC 
TTGCTGCGAG ACAAGATGCG GGCGCAGTGT CTTCTCCGCT TAGTGCTCGG AGTCACGGGA 
GATGGGGATG GCAATGATAT ATACAGTCTG TCGCTGGCAC CCTCCTTGCC TACATTACCC 
TTCGAGGTCT TCCGTCAGCT TCTTGATAGT GTGCCGTTAT CCACCGACGA CGGTGTTTTG 
CTACGACGCG TGGTAATCGA AGTGGGGGCC ATGCACCTAG TGCTTAATTG CCTCGGCATT 
TTCTCGCATC AGTCGCACAA CAACAGGATT GGAGCACCGA TTAGCGAACC ATCGCCAAGT 
GGAACCAGTA GTGGTAGCGG CACCATTGGC AATGGAAGTG GAAACGGAAG TGTAAGCGGT 
ACCAAAACTA ATGCACCAGA AGAGGCCACA CCTAGTGCCA CGTCGGACGA CAAAAGCCAC 
ATGTACTGGG CCAAGGGCAC TGGTTTCGGT ACCGGTTCTA CTACTCAGTC GTGGAATGTG 
GAGCAGGCGC TTCTTCGGCA AAAGAGCGAG GAGGAGCATG TCACAGTGTT GCTGCTTGTT 
CTAGCCAGTT ACATAAATCC GGGTGACTGT ATCCCAAGTG AAATGTGCGG AGAGGACATC 
CTAGCTTATC ATGATGTCCG AGATGTTACC GGCGAGTTGC CGCCCATCTT CCAAACCCTA 
CTGCAGCAGT CTTGCTTAAT CCCCGCCCTC AGCTCCTATC TGCGCAACGA TTCAGTGCTT 
GATATCACGC GCCACATTCC GCTATACCGT GCCATACTCA AACTTCTGCG TGCCCTATCG 
CTGTCTAAGA TGCTTGTCTC GTTGCTCCAA CCATCTGCCA GTGGCAGTGG GGACAGCACG 
CCACCCATAG TGGAGTTGCT CACAAGCATG AAGACCTGTG TGGATACCTA CGCCAAACGG 
TTAAAAGTTA ACAAGAAGTC AAACATAAAG GGACAGACAC ATCAGTTGAC CTTTAACATC 
GATGATGGCG ACGATGAGGG ATTAGCTTTG CTTATTCCGG ATATCCATGA AACCAGTGTG 
CTGGTGCAAA AAACCACAGA TGCTGATGCG CTAATCAACA TGTTGCATAC CAACGAAGAC 
GGCAGCGCAC AGCTAGAGCC CCAGAGCAAA TCAATTGAAC AGCGCTACTT AGAGCTAATG 
AAGAAGCGCC AGTTCGATAC GTTTGACATG ATTGTGGAGT CGGATAACAA CAGTTTCCGC 
TTTGTGGTCT CGCATCACTT CGAAAAAATG GTTCGGCTCG CTGGCGATCG TTACCATCCG 
TCGCGGGTCA AGCGACTTGC CCAGGAGGCA GTTACACTGT CCACCAGCCT ACCCCTCAGC 
TTTAGTAGTT CTGTATTTGT ACGCTGTGAT ACTGATAGAT TGGACATCAT GAAGGTACTC 
ATAACTGGTC CGGCGGACAC GCCTTACGCC AACGGGTGCT TTGAGTTTGA TGTCTTCTTT 
CCGCCGGACT ACCCTAACCA GCCCATGCTC ATCAATCTGG AGACGACGGG TCGCCATTCG 
GTGCGCTTTA ACCCAAACCT CTATAATGAC GGCAAAGTTT GCCTGTCAGT TCTCAACACC 
TGGCATGGTC GGCCTGAGGA GAAATGGAAT GCACAGACTT CAAGCTTCCT GCAGGTACTC 
GTTTCCATCC AGTCCCTGAT CCTCGTTCCC GAGCCCTATT TCAATGAACC GGGCTTTGAA 
AGAAGTCGTG GCAGTCCAAG TGGTACCAAT TCATCGCGAG AATATAATAG CAATATATAT 
CAGGCCTGTG TGCGGTGGGC TATGTTGGAG CAAATACGGA GTCCAAGTCA GTGCTTTAAA 
GATGTCATAC ACAAGCACTT CTGGTTGAAG AGAGAGGAGA TTTGCGCCCA GATAGAGGGC 
TGGATCGAGG AACTCGGTAA GCCGCAGTAC ACCGAGAGGG CCAGTCGCAC TATATCGTTC 
AACTCAATGG TCCTGCGCCG CCACTATCGC CACCTGCGTG AGGAGTTGTC GAAACTGAAG 
CCGCCACGTG GCCTCGAGGA CTTGGATGCG CCCTTTAATC CTGTGGCCAC GCTACCACCG 
ATGGATGTGT CCGCCGTGGC TCCTTCACTA GCAGCCACAA ATACCGTCCA AGCGCAACCG 
GATGATGCCA TAGTGACCGA TATTACCCTG CTCGGCGAGA ATGCGGATGA ATGCGAAGCT 
GATGGCGATG CCGAAGGCGA TGGAGAAGTG GACGATGGCT TTAAGGGTTT CCCGCAAGAA 
GAGGGTTTGC TGACGGGCGA GGAAGAGGTC GTGGAAATTT TAAGTGATAC GGAGAACGAG 
AGCAGTATGT GGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bruce ( NM_001275531.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262460.1 Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant B, mRNA.

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CloneID	OFa08258
Clone ID Related Accession (Same CDS sequence)	NM_001275531.2
Accession Version	NM_001275531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	BIR repeat containing ubiquitin-conjugating enzyme, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275531.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGATTCACCA ACGCGGCACA AGCGGCCGCA TCATGTTGAG 
GGATTTGTGG AACGTAACAA GGTCTCTAGA AACCCCTTTA ATGTGGCGGC AAATATAATC 
AGTGTGGTCA AGACAGCAGT CCTTGAAGTA TTTCCAGCGG CAAAAAACGC TTGCGTTAAA 
ATTATTCAAG TAAACAAACC CAAGACCCAA GACAATGAAA AGGTATTTGT GGCGAAGATC 
ATTAAAAACA AGACCCAAAG CCAGTCAGCT GCAGTTGCTC CCTCTGTTGG ACGCAGGCTC 
GAGCAACTGC CCAGTAGGGA AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCACTGCTT CCTCGTGTAC TGAGGCACTA 
TTTGAGACGC TGATGCCGAC AGCAAGAGAA TTTTATCAAG AGGCCGTCAA ATATAATTAC 
CTACCTTCAT TAATTGAATG CGATGACACG GAATTCGATG AAATGCTAAA CGACATTGAC 
ATGATTGAAC GAACGAAGCC AGTTACCAAA AAGGAAAGTA ATGCCCTGTC TAAGAATACA 
TTCAATTTCT TCTGCAAGTC TATCTCGATT GCTCTGGATT CACGATTGGA CGTGGGTCTG 
GAATTGCACG TAGAAACTCA GCTGAGAAGG CTAACAAATC GCACATCCCT GGATCTCTAT 
TCATCGCTTC CTCAGGTGTT GACCAATGAG TTTGTCTCTC CACCACCTGA GGCTGCTCCT 
TGGCCAGAAT ATTTGACTCA ACTTTGGTCA GGCACGGAAT ACAACGGTGA CAATACTTAT 
GTGATGTTTA AAAGGGTCTT TGACTCCATC CTGGCGGAAT TGCACCTTGA AGACACGTGG 
GTGCACTTGG AGCAGGTGCT GCAGATGTGG CTGACGCTAA ATTGCGAGCT CTCGGACAAG 
CCATACACCA GTGGCATCAC TCACTCGGAT GTTCCAAAAA TTCCGTTTGG AGCAAATGCG 
GTGCAGGGAT TGCTCCTGGC TTTGGCTTGG CACAGTGATA TAAAACTGCG GACCTGGTGT 
CTTGGCTTTC AGTGCCTGTT TCTCGCTTGC AACTCATTGC CTATCGGAGA TGATGCGGAT 
TGTACGCGTA TCAACGAGGC TATCGTGAAT GATGAAAACT TTGAAAAAGT GCTGCTACGA 
TTCTTTTCCG GTTATGGCAT GAGCTCATCT ATCATCACCA ACCGATGCGC TGGACCTACT 
ATTTGCAAGC ACTTGCATGA GTTATTGCTC TGGCTGCACA GCAAAAGCGA GACGAGTGGC 
ATTCCGTGCA AGCGTCGACT TAAGGACATT TTGTTGCATG TTGTGCTTCA GTTGGTGCAG 
CCTGGTGGCT CTATTAGCAA CCAGCAGGGT CCAATTGATG CTCAAAACCA GCTGGTTCGT 
GATTTGCTTA TGATGCCCAA TGATAAGGGG GACCTCAACA TTGCGCTTAA AATCACAGAA 
AGTGTTTCTT TCCTGGTGTA CAACAACATA TCCAATGGCG AGAGGCTACA ATGTCAGCGT 
GGAAATGAAA ACAGCCCAGT TGGCAACAAT TTTAGCAATT TGTTTGCCAA CGTTCTGGGA 
TCAGAAAATC CCAACAAGCA GAATGCCGCA ATTTGCGACA ACTCACTTAT TATAAATCTG 
CTGAAACTAT CGTCACACAT GGTATTGACA GAGATGCCCC GGCAACCAAG TGAGGTTACC 
ATTCCCGAGG AGGAGGAACT TTCCAATCAG AGTCAAACGG ATGAGACTAA AGCGGAGCAG 
TTAAACACGG AAGGACATCG CAGCAAAGTG CCTTGCATTG CAGATTGGGT ACTCCGCCAT 
TTCCCCACAA TGAAGCGACT TTTTGGAACG CTTTCCCAGT GCTCGACAAA CACCTTCACC 
ATGATGTCCT CTGGCTGTTT CTTTTCACCG AAGTCCAATA TGATTTTAGA TGACCCGCAG 
ACAATTGCAG ACGCCGTCTT TAGTTTGATG ATTACTCTCA ACGACAAGGC TTCGCATCCC 
CGACTTGTTG TGGTGCCCAT TTGCCGATAC CTTGAAGAAA CCACTATTCC GAGAAATGCA 
ACGCTGCCGC GTCTGCCGCA TTCTGAACCT TTCCTGTGGT ACATCTCGAA GGTATTGGAA 
GTACCAGCGG CAGTGCAAAC GTTCACCCAA TTGGGCGGAA TTCAGATTAT ATGTCATAGC 
CTGGTGCGAC TTAATAAAAC TATCATCAAC ATGCAACCAG GATTGATTTC TTTGATTATG 
CAACACTTGA CGCGCAATAC GCGCTTTAAG CGTAATAATC ATGTGGCGGC AAGTAGTGCT 
TGCAAAAAGA CAACGACTGG TACGGGAACC ACATGTCCCA CGTCAGCACA GCCGCCGAGA 
AGTGCTGCTC AGTACGATGG TCTGATCAAC TTTGCACCCT TCTCGCATAT TGTCTCCGAA 
AATGATGCGA CACAAAGTGC AGAGGTACTA ATATCAAATC CAAATAATTC ACATCGAAGA 
CCGCGATCGC CAGCCTGGAC CTATATGTTC TATCCAAATG AGACGCATGT GGATCTGACT 
ATAACGTTGC CCACAGCAAT TTTGTTGAAA GAGGTGCAGC TGGTGCCGCA CACAACTAGT 
TTGGCATCCT GTCCATCTGC GGTGGCTCTA GAGCTGTCTA GAGATTACGG CCTTGGTTCG 
ATCCCAGTGG GTGCTCCCAT GGCCACCACT GGTCTGACCT GCATTAGACT AAAGCTGGCC 
AAGGCAGAGG TGGCTACCAG CATTGTTCTG AGGCTGTACA AACCCAAGGA CTGTGGAAAT 
GTGGGTCTCG TACAAATCGC TGTGCTTGGT CAGACAATCT TCGGAAATCG CATGCATGGC 
CTTCGCTCAC CGAGTCCGTT CATTGACTCC CTAGCAACGT GCTCCTCGCC AGTGCAAACG 
GCAGTTTTAG CCGCAATGGA TGCGGATTCC ATGCTTGAAG ATGATGCTTT TGCGAAGACT 
AGCATTGGCT GGGTTCGCAT TCTGTCGCGC TGTTTCCATG TGGCCGCTTT ACAACCAGAC 
AGTAAGCTGG CTGATCTTAT CGCCGCCTAT CCGGCTGCAT ATCCCGGCTT TTTAGAGGCC 
TGCTGCTCAT TGCTAAACAT AATGCCAATG ATTCCTAGTC TTGCACAGCA ACACTTGGAG 
ACCATTTTGT TAAAATTAAG TGCATACAAC CACGAGATCA GTTTGCAACT TCTTAGGACG 
CTCCTGTGGC ACACCACTCC GCAAGTTTTT AAACTCTCCA GCGATTCGGT GTGTGATCTT 
ATTTATGAAG TAGTTACCGG ATCGACGGAG CACCTACTGG ACAAACTACG CGTGCTTATC 
GACTGGGTAA TGCAATTGCA CGACAACTAC AGCAAGCAGG CGCGCTGTAA TAACCCACAA 
AGTGGATTTG TTAAGGTGAT TGCTTCGATT CTGTGGAAAG TGCATACCCA GCAACAATTG 
CCTGAGTTAC CGCTTCTCAT TTCATCTTCG CTGTTCGAGA CCTGTTTTGA ATGGGTCTCT 
TCGCTGGAGC ACGATGAGCC ACTGAAGAGC AGTTTTGACT CCTTGTTGTG TGCATTGTGC 
TTCATTAAGC CAGAACTGTT TAACCAGCTG TTGGTCAAGC TGGGAGTTAA GCTGGAACCT 
CCAGCTCGAG GTCAAACTGA TGACAGCAAA GTGCAAAGTG GTAGTGCTTG GTTCCGCCGT 
GAGGGTAGCG AAAACCTTAG TGTTTTGCTA CAGCGTCCCA CATTCCTAAA GACTCTAGCC 
TTGGCCTGCC AGTCCCCTGC AGCAGTTTAC CAGATGCTAG ACTCCGGACT TCCAAAGCTA 
TTGGCCCACG CCATTTACGA GTATTGCATG CATCTCATGC CGGGATCGGA GCCAGTAGCT 
TCTGCATCTA CAGTGCCGGA AGACGAACCG TTAGCCAACA ATCAGGCAGC CGCTGCAGCA 
GATGACGAGG ACTCGTCTCT TACGGACTTT GACAAGGCCG AGGCAAATGT GGTTTATAAT 
ACTCCACTGC TAAGTAGCTC GAATGTACCC AAAGTGCTAG ATTTCTTTGC CGAATGTTGT 
GCCGAAGGTC CAATGCGTGA TTGGTTAGGT ACTATGCAGG GCTCCGTATT TTGGAAGCCT 
CTGCTGCAGT TGCTCTGTAA CACACATGCC GCCCAGTTCA GCAAGACGTT GCCTAAGGAA 
CAATCGTTTA TCCGCCTGGA GAGGGCCACA ATCAACTTCT TTACGCGAGT TAGCGCCTGC 
CATCCCGGAA ACCAGGAAAA TCTTACATCG TTGCTCATTG GAGCCATAAT TTGCAAACCA 
TTAAGATTTT CGCATGGAGT TCGACCAACT ATATCTGGAT TTACTAGACA GATGGTGCTG 
CAGCTCCTCC TCGAGAATGA ACACATCACA GTCTCTGTTA GAAGTCAGCA GCCCTTGCAA 
CGGAGGGATA CCCTCTCCGC CGTTCTAGGC GGGATTTCGA GTGGTGCAAG TACTAGCTTA 
CCCGTAGCAG CAGTCAACAA TCATCCGGCC AGGAGGAGCA GCGCCCACCA CATGCTATTC 
ACGGTGACCA CTAGCACCAC GTGCCAAGAG ATTATGCAGA ATTGCGTTAG TGTGTTTAGT 
AATCTAGTTT ATCAACAACC AACAGATCAG CGAGCTGGCG AGACCTCTGC CTCGAGCAGC 
GGCGTATCGA GTGCAACTGC CAAATCCAGT GAGAGCAAAA TGGAAAAGAG CAGCTTCCTG 
AACTTTAACA ACGTAGAAGC GGGTAGCATG GAGTTCCTTA CCGTTGGTGC TGCTGTGGCA 
GCCAAGGACA AGCGCATAAA AGATGCTAAG AACCAAGCAG CCATGAACAA GGACAAGGAG 
ACTCAGACCA GCTCAACGAA CTTGGCTTCC AATATATTCA ATGTGGATGA GTTCTTGTTG 
CAATCGGCGA ATGCCGTTAA TGGTAACCAG TTGGTCATTC CAGAGTATTC TGATATTGTT 
CTAGCTGGAG ATGCCACCAT CTCGCAGGTT TTGGCCTCGC TTCAGAATGC AGGACATTCT 
CTTTCGACGC CCTGTATATC GTTTGATCTG GTACTTCAAC AGCGCTCTGA TGACACAAGC 
GAGGCAAAGA AGACCAAAGC TGCCTGCACT GAGCCGTTGC CATCACCACT GCAACGATTT 
TCCAGCAGTG GTGGTCTATC TTTACTCGCT CACTACCTGC CCACCGTATA TCCGGAACAC 
TCAGGTCGCA AGACCACCTT GGTGTTTAGC GAGAAGGAGA AAAGTCCCCC GCTCTCTGAC 
TGGGTGAAAT TGGAACCGAA CGACGAGATC TATGAGGATT TGGAGGACAC TCTCTGTGAG 
CCAGCAGCTA AGCTGGCAAC GGTCAGTTCG GTGCCGCAGC ACTCGCTGGC CGCATTTGGA 
TTATTCCTGC GCCTGCCCGC CTACTCTGAT GTCTTGCTGC GAGACAAGAT GCGGGCGCAG 
TGTCTTCTCC GCTTAGTGCT CGGAGTCACG GGAGATGGGG ATGGCAATGA TATATACAGT 
CTGTCGCTGG CACCCTCCTT GCCTACATTA CCCTTCGAGG TCTTCCGTCA GCTTCTTGAT 
AGTGTGCCGT TATCCACCGA CGACGGTGTT TTGCTACGAC GCGTGGTAAT CGAAGTGGGG 
GCCATGCACC TAGTGCTTAA TTGCCTCGGC ATTTTCTCGC ATCAGTCGCA CAACAACAGG 
ATTGGAGCAC CGATTAGCGA ACCATCGCCA AGTGGAACCA GTAGTGGTAG CGGCACCATT 
GGCAATGGAA GTGGAAACGG AAGTGTAAGC GGTACCAAAA CTAATGCACC AGAAGAGGCC 
ACACCTAGTG CCACGTCGGA CGACAAAAGC CACATGTACT GGGCCAAGGG CACTGGTTTC 
GGTACCGGTT CTACTACTCA GTCGTGGAAT GTGGAGCAGG CGCTTCTTCG GCAAAAGAGC 
GAGGAGGAGC ATGTCACAGT GTTGCTGCTT GTTCTAGCCA GTTACATAAA TCCGGGTGAC 
TGTATCCCAA GTGAAATGTG CGGAGAGGAC ATCCTAGCTT ATCATGATGT CCGAGATGTT 
ACCGGCGAGT TGCCGCCCAT CTTCCAAACC CTACTGCAGC AGTCTTGCTT AATCCCCGCC 
CTCAGCTCCT ATCTGCGCAA CGATTCAGTG CTTGATATCA CGCGCCACAT TCCGCTATAC 
CGTGCCATAC TCAAACTTCT GCGTGCCCTA TCGCTGTCTA AGATGCTTGT CTCGTTGCTC 
CAACCATCTG CCAGTGGCAG TGGGGACAGC ACGCCACCCA TAGTGGAGTT GCTCACAAGC 
ATGAAGACCT GTGTGGATAC CTACGCCAAA CGGTTAAAAG TTAACAAGAA GTCAAACATA 
AAGGGACAGA CACATCAGTT GACCTTTAAC ATCGATGATG GCGACGATGA GGGATTAGCT 
TTGCTTATTC CGGATATCCA TGAAACCAGT GTGCTGGTGC AAAAAACCAC AGATGCTGAT 
GCGCTAATCA ACATGTTGCA TACCAACGAA GACGGCAGCG CACAGCTAGA GCCCCAGAGC 
AAATCAATTG AACAGCGCTA CTTAGAGCTA ATGAAGAAGC GCCAGTTCGA TACGTTTGAC 
ATGATTGTGG AGTCGGATAA CAACAGTTTC CGCTTTGTGG TCTCGCATCA CTTCGAAAAA 
ATGGTTCGGC TCGCTGGCGA TCGTTACCAT CCGTCGCGGG TCAAGCGACT TGCCCAGGAG 
GCAGTTACAC TGTCCACCAG CCTACCCCTC AGCTTTAGTA GTTCTGTATT TGTACGCTGT 
GATACTGATA GATTGGACAT CATGAAGGTA CTCATAACTG GTCCGGCGGA CACGCCTTAC 
GCCAACGGGT GCTTTGAGTT TGATGTCTTC TTTCCGCCGG ACTACCCTAA CCAGCCCATG 
CTCATCAATC TGGAGACGAC GGGTCGCCAT TCGGTGCGCT TTAACCCAAA CCTCTATAAT 
GACGGCAAAG TTTGCCTGTC AGTTCTCAAC ACCTGGCATG GTCGGCCTGA GGAGAAATGG 
AATGCACAGA CTTCAAGCTT CCTGCAGGTA CTCGTTTCCA TCCAGTCCCT GATCCTCGTT 
CCCGAGCCCT ATTTCAATGA ACCGGGCTTT GAAAGAAGTC GTGGCAGTCC AAGTGGTACC 
AATTCATCGC GAGAATATAA TAGCAATATA TATCAGGCCT GTGTGCGGTG GGCTATGTTG 
GAGCAAATAC GGAGTCCAAG TCAGTGCTTT AAAGATGTCA TACACAAGCA CTTCTGGTTG 
AAGAGAGAGG AGATTTGCGC CCAGATAGAG GGCTGGATCG AGGAACTCGG TAAGCCGCAG 
TACACCGAGA GGGCCAGTCG CACTATATCG TTCAACTCAA TGGTCCTGCG CCGCCACTAT 
CGCCACCTGC GTGAGGAGTT GTCGAAACTG AAGCCGCCAC GTGGCCTCGA GGACTTGGAT 
GCGCCCTTTA ATCCTGTGGC CACGCTACCA CCGATGGATG TGTCCGCCGT GGCTCCTTCA 
CTAGCAGCCA CAAATACCGT CCAAGCGCAA CCGGATGATG CCATAGTGAC CGATATTACC 
CTGCTCGGCG AGAATGCGGA TGAATGCGAA GCTGATGGCG ATGCCGAAGG CGATGGAGAA 
GTGGACGATG GCTTTAAGGG TTTCCCGCAA GAAGAGGGTT TGCTGACGGG CGAGGAAGAG 
GTCGTGGAAA TTTTAAGTGA TACGGAGAAC GAGAGCAGTA TGTGGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262460.1
CDS	167..15097
Translation	MATEQHHQQRVLKQDGYLDLGDCNSAPSRPFEMRNVVYHPQLNVLLLFDNGSTVKVLDANSGVILQTYHLSSNGDSIVHGRYMPLQDKILFWDGHNLGLRGDYNGVLLLDTILQAPIGQNDDYIKLEIILSEAIIFQNCITELESEGLECPCDISNELTQKINQAQLKAKKGIKAQRWNTICLEVPYSSLKLVSNNMVILLKRLERHIPVLAIASAINERLTDMMMGSRVPDFGWNFSNFQRVLMHSEAVRRQTFEKWPHMDYKWALPDQMAQAGFYHQPSSSGEDRAMCFTCSVCLVCWEKTDEPWSEHERHSPLCPFVKGEYTQNVPLSITYATNPALPAPGLGFDIISNSDYANVLCTSCSQTGELSVWSIERHLKLMHTFHVPTLLNYIFEESFEWARVTAICVLPNARARTKVNYVYSAANYGGSGSSNGAGCNSGLNAVSGGKFGSVNAQHITSTSTLRAGVVGSKIVLGVSVRQSSGELALRMVVLNIVEVDRNSESSTSVSNDSGSVSSGGGQLMKSSSPMVNVSAENNATVTQVNLESKDDDNKAMTPYEKFADGFDSNGFVSALMTYNKNNGVSQNTDDASALYDFDLCTLSQILDHNLEEPLSLMVGSSNTVNVSNIGMGNEINKLVVDVEISHQNNNNNNNNNNEASSLGGNSSNNNNNIMINGGTNSSIDKMTAIEEIDCVVESWTSVKRMNALDGASSGVNGVNHMDQEVIEIAEILPTTPKCNQLLVKLLRTKAPEAEGSKIIMATVAKEEEEEDEEQRTDESMETDNNAEGNVAAQLLVFDYDDTEISNDYTLAMTFSRAKFPKQLCILPHFNSSPEHKDAGSICVVCADGTVEIYSLADFQGTMVIEEEGEHFESVVYCRNLDRLCCCSRQGGLLFYSLSDGENDSGDELLEMDDDCSTTLTQAADVSVTDGGRQLARTDLLGKTLLANESICMDAGSEAAAIPNSSSAAQGAHVQTSPSASSAPSTNANLLAYKTADLTLEDLKVFYALTQFDDKLTVYSAEVPSCWNDIGQVQNQRKQSQNMRHGGDERDFTRTWRLHNDATTWDEHIIELNLAQPVSLGHIDLKFTVYQQCSNPAAIQVTLLKQNNNGFGYRMKGPHSSYKPNVIDDNIDLSYIANENPVLSEEYLLAHNAEVLAGPIELSSCIDLCDQGGTATLTSPKLFKTRTRNFLLHIKTVSDPSKDGQNKTRDSPTRHKRPHHVEGFVERNKVSRNPFNVAANIISVVKTAVLEVFPAAKNACVKIIQVNKPKTQDNEKVFVAKIIKNKTQSQSAAVAPSVGRRLEQLPSREGCDWLHEISISVRAVKSHSRISNGRMQRIAMLESNVLLVQLLRIASDPASSSLAKNLSLDILNWICFIRLNRFRSPKSERLKDQINKQTAANIQDLLAQQFECISLAEKHIEQLLRNCVIYSERSTAHKCVKLLIMLTEGATNLPAEVQDHTTLDYSFKAAIANTFPELPRAQCGSVVRWYMMLTCATSTAESHNSISEQAISLLKEIAAEIDKRWDPHCALLCTRFGLYGYPFEPEIFDFDFPNVSRQGPGSSVANVIRSSPTILPVTGLDIKRLSSIDGVDFRTFPYLIRGKSISNQLRGLLEVVQLHFNCVQTSEATRIDNIDSVGGNTGANVIIEDVMMMPKQVVAKEKGEEMLNDLVNDVECLVEEMKVDENEKQEKVDYDAFGLVNGFKDKTLSSLNSVKQTLKEEVKKEKADIMDKLTIAKFLPKLSLLESYMQKKINEHISFDDFGKLDNWDGNTTPPFNLEVEAPIVCSEATSESNMSQQATSAGGNILDDAADSEKITESLNANPCQPIAWHKLITPPPKQMIVIDRMHSGARRFVVLDFGQHILLTDLVIPSCDELTSLNIDIWCFDEETDCTRLVQVNDIQTKTLVLSDIQPAPICRYLKLTIIGRIGMSATKCKVPLGRFFGHPVVLEHDGYGDQLMKYIKHPTHSLQSQIKSLNALYEDVHCRYSLASSKLMDLLAPMRNSELSNVAHLQAFINKQREEELSGLENNNKVVSLYDECMLLQYQINVIRNVVLRLERALSPNSPTTTLLPGQSIPLTEDILRAASRDKLRVLSLSLVEVLLHFSIEYGIKNILPVHQRFNAATAKALFNALVVYGDAQLQLATCSLLVRMCCFQPWWGDFLADTFCSLFSAQNCKAFPQDRIFFLLTYLGRRSIAMGACRSIVIDAVLKTLVKLLAPISPHYKFQSEQPSTSANAADRNAAMGTTSDLQLITWLLLFLSVCLDDNERKDKSAARWDFMSCEGDFTKARPSATPNIKQLRCFKKRIIQQNKYLAQPYADWGKKIYMIQNEHPGIFMELSTKSKGAGPTKSTSTGGKINMGSSGLCVGGPSSSSGNTSKSAASSSSPKNQAVPGTDGERDSNFDKGLKSLRMDNIKVVIRGLFALLLEMDFDCNMDQFLLTCKVIARLVAACRPSVQLCKIVTIAQLQQLVRLSVWNDQQHPWAVHAITCLLQDLLDADKQFRDNDVTPTNDTPRTMEAAIQETRDLFSDYTSTASSCTEALFETLMPTAREFYQEAVKYNYLPSLIECDDTEFDEMLNDIDMIERTKPVTKKESNALSKNTFNFFCKSISIALDSRLDVGLELHVETQLRRLTNRTSLDLYSSLPQVLTNEFVSPPPEAAPWPEYLTQLWSGTEYNGDNTYVMFKRVFDSILAELHLEDTWVHLEQVLQMWLTLNCELSDKPYTSGITHSDVPKIPFGANAVQGLLLALAWHSDIKLRTWCLGFQCLFLACNSLPIGDDADCTRINEAIVNDENFEKVLLRFFSGYGMSSSIITNRCAGPTICKHLHELLLWLHSKSETSGIPCKRRLKDILLHVVLQLVQPGGSISNQQGPIDAQNQLVRDLLMMPNDKGDLNIALKITESVSFLVYNNISNGERLQCQRGNENSPVGNNFSNLFANVLGSENPNKQNAAICDNSLIINLLKLSSHMVLTEMPRQPSEVTIPEEEELSNQSQTDETKAEQLNTEGHRSKVPCIADWVLRHFPTMKRLFGTLSQCSTNTFTMMSSGCFFSPKSNMILDDPQTIADAVFSLMITLNDKASHPRLVVVPICRYLEETTIPRNATLPRLPHSEPFLWYISKVLEVPAAVQTFTQLGGIQIICHSLVRLNKTIINMQPGLISLIMQHLTRNTRFKRNNHVAASSACKKTTTGTGTTCPTSAQPPRSAAQYDGLINFAPFSHIVSENDATQSAEVLISNPNNSHRRPRSPAWTYMFYPNETHVDLTITLPTAILLKEVQLVPHTTSLASCPSAVALELSRDYGLGSIPVGAPMATTGLTCIRLKLAKAEVATSIVLRLYKPKDCGNVGLVQIAVLGQTIFGNRMHGLRSPSPFIDSLATCSSPVQTAVLAAMDADSMLEDDAFAKTSIGWVRILSRCFHVAALQPDSKLADLIAAYPAAYPGFLEACCSLLNIMPMIPSLAQQHLETILLKLSAYNHEISLQLLRTLLWHTTPQVFKLSSDSVCDLIYEVVTGSTEHLLDKLRVLIDWVMQLHDNYSKQARCNNPQSGFVKVIASILWKVHTQQQLPELPLLISSSLFETCFEWVSSLEHDEPLKSSFDSLLCALCFIKPELFNQLLVKLGVKLEPPARGQTDDSKVQSGSAWFRREGSENLSVLLQRPTFLKTLALACQSPAAVYQMLDSGLPKLLAHAIYEYCMHLMPGSEPVASASTVPEDEPLANNQAAAAADDEDSSLTDFDKAEANVVYNTPLLSSSNVPKVLDFFAECCAEGPMRDWLGTMQGSVFWKPLLQLLCNTHAAQFSKTLPKEQSFIRLERATINFFTRVSACHPGNQENLTSLLIGAIICKPLRFSHGVRPTISGFTRQMVLQLLLENEHITVSVRSQQPLQRRDTLSAVLGGISSGASTSLPVAAVNNHPARRSSAHHMLFTVTTSTTCQEIMQNCVSVFSNLVYQQPTDQRAGETSASSSGVSSATAKSSESKMEKSSFLNFNNVEAGSMEFLTVGAAVAAKDKRIKDAKNQAAMNKDKETQTSSTNLASNIFNVDEFLLQSANAVNGNQLVIPEYSDIVLAGDATISQVLASLQNAGHSLSTPCISFDLVLQQRSDDTSEAKKTKAACTEPLPSPLQRFSSSGGLSLLAHYLPTVYPEHSGRKTTLVFSEKEKSPPLSDWVKLEPNDEIYEDLEDTLCEPAAKLATVSSVPQHSLAAFGLFLRLPAYSDVLLRDKMRAQCLLRLVLGVTGDGDGNDIYSLSLAPSLPTLPFEVFRQLLDSVPLSTDDGVLLRRVVIEVGAMHLVLNCLGIFSHQSHNNRIGAPISEPSPSGTSSGSGTIGNGSGNGSVSGTKTNAPEEATPSATSDDKSHMYWAKGTGFGTGSTTQSWNVEQALLRQKSEEEHVTVLLLVLASYINPGDCIPSEMCGEDILAYHDVRDVTGELPPIFQTLLQQSCLIPALSSYLRNDSVLDITRHIPLYRAILKLLRALSLSKMLVSLLQPSASGSGDSTPPIVELLTSMKTCVDTYAKRLKVNKKSNIKGQTHQLTFNIDDGDDEGLALLIPDIHETSVLVQKTTDADALINMLHTNEDGSAQLEPQSKSIEQRYLELMKKRQFDTFDMIVESDNNSFRFVVSHHFEKMVRLAGDRYHPSRVKRLAQEAVTLSTSLPLSFSSSVFVRCDTDRLDIMKVLITGPADTPYANGCFEFDVFFPPDYPNQPMLINLETTGRHSVRFNPNLYNDGKVCLSVLNTWHGRPEEKWNAQTSSFLQVLVSIQSLILVPEPYFNEPGFERSRGSPSGTNSSREYNSNIYQACVRWAMLEQIRSPSQCFKDVIHKHFWLKREEICAQIEGWIEELGKPQYTERASRTISFNSMVLRRHYRHLREELSKLKPPRGLEDLDAPFNPVATLPPMDVSAVAPSLAATNTVQAQPDDAIVTDITLLGENADECEADGDAEGDGEVDDGFKGFPQEEGLLTGEEEVVEILSDTENESSMWQ

Target ORF information:

RefSeq Version	NM_001275531.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster BIR repeat containing ubiquitin-conjugating enzyme (Bruce), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275531.2

ORF Insert Sequence:

ATGGCCACGG AGCAGCATCA CCAGCAGCGC GTCCTCAAGC AAGATGGCTA CTTGGATTTG 
GGCGACTGTA ATAGCGCTCC ATCTCGACCA TTCGAGATGC GCAACGTCGT CTACCATCCG 
CAATTAAATG TCCTTTTGCT TTTTGACAAC GGAAGCACGG TCAAGGTCCT GGACGCCAAT 
TCAGGAGTGA TTCTGCAGAC CTATCACCTC AGTAGCAATG GCGACAGCAT TGTGCATGGT 
CGATATATGC CTCTGCAAGA CAAGATCTTG TTCTGGGATG GTCACAATCT CGGATTGCGA 
GGCGATTATA ATGGCGTGCT GTTGCTGGAC ACCATTCTAC AGGCTCCAAT TGGCCAAAAC 
GACGACTACA TCAAGCTGGA GATCATCCTG TCCGAAGCGA TCATCTTTCA GAACTGCATA 
ACCGAACTGG AAAGTGAGGG CTTGGAGTGC CCCTGTGACA TTAGCAACGA GCTGACGCAA 
AAGATAAACC AGGCGCAGCT GAAAGCCAAG AAGGGCATTA AGGCCCAGAG GTGGAATACC 
ATATGTCTGG AGGTACCCTA TTCTAGCCTA AAGCTAGTAT CCAATAATAT GGTTATCCTA 
CTGAAGCGGC TGGAGCGCCA CATTCCCGTG CTGGCCATCG CTTCCGCCAT TAATGAGCGT 
CTGACCGACA TGATGATGGG CTCCCGCGTG CCAGATTTTG GCTGGAACTT TAGCAATTTT 
CAGCGCGTAC TTATGCATTC GGAGGCAGTG CGACGCCAGA CGTTTGAGAA GTGGCCCCAT 
ATGGACTACA AATGGGCTCT GCCCGATCAG ATGGCTCAGG CCGGTTTTTA CCATCAGCCA 
TCTTCATCTG GAGAGGATCG CGCCATGTGC TTCACCTGCA GCGTGTGTCT TGTGTGCTGG 
GAGAAGACGG ATGAGCCGTG GAGCGAGCAT GAGAGGCACT CACCACTGTG TCCATTTGTC 
AAGGGCGAAT ATACACAGAA CGTCCCGCTG TCCATAACCT ACGCCACTAA TCCCGCATTG 
CCAGCACCAG GACTTGGGTT CGATATCATT TCAAACAGTG ATTACGCGAA CGTGCTCTGC 
ACCAGTTGCT CGCAAACGGG AGAGTTGAGC GTGTGGAGCA TTGAACGCCA TCTGAAGCTG 
ATGCACACCT TTCATGTTCC GACACTGCTT AACTACATTT TCGAGGAGAG CTTCGAGTGG 
GCACGCGTTA CTGCCATATG TGTGCTACCG AATGCACGAG CTCGCACCAA GGTGAATTAT 
GTGTACTCGG CGGCTAATTA TGGCGGCTCA GGAAGCAGCA ATGGAGCGGG ATGCAACTCG 
GGATTGAATG CTGTATCTGG TGGAAAGTTT GGCAGTGTGA ATGCCCAGCA TATCACCTCC 
ACGTCCACGC TTCGGGCCGG CGTTGTTGGA TCCAAAATTG TTCTGGGAGT CAGCGTGCGC 
CAGAGCAGCG GAGAATTGGC CTTGCGCATG GTAGTGCTTA ACATCGTGGA AGTGGACCGC 
AACAGTGAGA GCTCAACGAG CGTCAGCAAT GATAGCGGCA GCGTCTCAAG CGGTGGAGGC 
CAACTGATGA AGTCTTCTTC TCCGATGGTC AACGTGTCCG CTGAAAACAA TGCCACAGTG 
ACCCAAGTGA ATCTTGAGTC CAAAGATGAT GATAACAAGG CTATGACACC ATACGAGAAG 
TTCGCCGATG GCTTCGATAG CAACGGATTT GTGTCCGCTC TTATGACCTA CAACAAGAAT 
AATGGAGTTA GCCAAAACAC CGACGATGCC TCAGCCCTGT ATGATTTCGA TTTGTGCACA 
CTGTCCCAGA TTCTGGATCA CAATCTGGAG GAGCCTCTCA GTTTGATGGT GGGATCATCG 
AATACGGTGA ATGTCAGCAA TATTGGCATG GGCAACGAGA TTAATAAGCT GGTCGTGGAT 
GTGGAGATTA GTCACCAAAA CAATAATAAT AATAATAACA ACAACAACGA GGCGAGTTCT 
CTGGGAGGAA ATTCCTCTAA TAATAACAAT AACATCATGA TCAATGGAGG CACTAACAGC 
AGTATCGACA AAATGACAGC CATCGAAGAG ATAGATTGTG TAGTTGAGAG CTGGACAAGT 
GTGAAGCGCA TGAACGCTTT AGATGGTGCC AGCAGTGGGG TAAACGGTGT GAACCACATG 
GACCAGGAGG TAATCGAAAT TGCCGAAATT CTTCCGACTA CTCCCAAGTG CAACCAACTG 
CTGGTCAAGT TGCTGCGTAC CAAGGCACCG GAGGCAGAGG GAAGCAAAAT CATCATGGCT 
ACCGTGGCCA AGGAGGAGGA GGAAGAGGAT GAGGAGCAGC GGACAGACGA GTCAATGGAG 
ACAGATAATA ACGCCGAAGG GAATGTAGCA GCTCAGCTCT TAGTGTTCGA CTACGATGAC 
ACTGAAATAT CCAACGACTA CACCCTGGCC ATGACTTTCA GTAGGGCCAA GTTCCCCAAG 
CAACTGTGCA TTCTGCCGCA CTTCAACTCT TCTCCAGAGC ATAAGGATGC CGGGTCCATA 
TGTGTGGTGT GTGCAGATGG CACCGTGGAA ATATATTCTC TCGCTGACTT CCAAGGCACA 
ATGGTCATCG AGGAGGAAGG CGAGCACTTC GAGTCTGTGG TTTATTGCCG GAACTTGGAT 
AGGCTATGCT GTTGCTCTCG ACAGGGTGGC TTGCTCTTCT ACTCGCTAAG CGATGGGGAG 
AACGATTCTG GAGACGAACT TTTGGAAATG GACGATGACT GCAGTACCAC GTTAACACAG 
GCAGCGGACG TAAGTGTTAC GGATGGCGGA CGCCAATTAG CCAGAACGGA TCTACTGGGC 
AAGACCCTGC TCGCCAACGA ATCTATTTGC ATGGATGCTG GCAGTGAGGC AGCTGCAATC 
CCGAACTCCT CCAGCGCGGC TCAGGGTGCG CACGTCCAAA CTTCGCCATC GGCATCTTCC 
GCGCCATCCA CCAATGCGAA CCTGCTCGCT TACAAGACGG CTGACCTGAC ACTCGAAGAC 
CTAAAAGTCT TTTACGCCCT CACCCAATTC GATGATAAAC TAACCGTCTA CTCAGCTGAA 
GTTCCCAGTT GTTGGAACGA TATCGGGCAG GTGCAGAATC AACGTAAACA GTCCCAAAAC 
ATGCGACATG GTGGCGACGA ACGAGATTTC ACTCGCACCT GGCGTCTTCA CAACGATGCA 
ACCACCTGGG ACGAGCACAT TATAGAGCTT AACTTGGCAC AACCCGTATC TCTGGGACAC 
ATTGATCTAA AGTTTACGGT CTACCAGCAA TGCTCAAACC CTGCAGCAAT TCAGGTTACG 
TTATTGAAAC AAAATAACAA TGGATTTGGG TACAGAATGA AGGGACCTCA CTCCAGTTAT 
AAGCCGAATG TCATCGATGA CAACATTGAT CTCTCATATA TTGCCAACGA AAATCCTGTG 
CTGAGTGAGG AATATTTACT AGCCCACAAT GCGGAAGTTT TGGCCGGTCC CATTGAACTC 
TCCTCTTGCA TTGATCTGTG TGATCAGGGA GGCACCGCCA CGCTCACATC CCCAAAACTA 
TTTAAGACCC GTACTAGAAA CTTCTTGCTC CACATCAAAA CAGTGTCGGA TCCGTCGAAA 
GATGGGCAGA ACAAGACTAG AGATTCACCA ACGCGGCACA AGCGGCCGCA TCATGTTGAG 
GGATTTGTGG AACGTAACAA GGTCTCTAGA AACCCCTTTA ATGTGGCGGC AAATATAATC 
AGTGTGGTCA AGACAGCAGT CCTTGAAGTA TTTCCAGCGG CAAAAAACGC TTGCGTTAAA 
ATTATTCAAG TAAACAAACC CAAGACCCAA GACAATGAAA AGGTATTTGT GGCGAAGATC 
ATTAAAAACA AGACCCAAAG CCAGTCAGCT GCAGTTGCTC CCTCTGTTGG ACGCAGGCTC 
GAGCAACTGC CCAGTAGGGA AGGCTGCGAC TGGTTGCACG AAATATCCAT CAGTGTGCGT 
GCTGTGAAGT CTCACAGTAG AATCAGCAAT GGACGGATGC AGCGCATAGC CATGTTGGAG 
AGCAACGTGT TGCTGGTGCA ACTGCTACGC ATCGCTAGTG ATCCGGCCTC CAGCTCGTTG 
GCCAAGAACC TATCACTTGA TATACTCAAT TGGATTTGCT TCATCCGGCT GAACCGTTTT 
CGTTCACCCA AATCGGAGCG GCTCAAGGAC CAGATTAATA AACAGACAGC CGCTAACATC 
CAAGATTTGC TTGCTCAGCA GTTCGAGTGC ATTTCCCTGG CTGAGAAGCA TATCGAGCAA 
TTGCTGCGGA ACTGCGTTAT CTACAGCGAA CGTAGTACGG CCCACAAATG TGTGAAACTG 
CTGATCATGT TGACAGAGGG TGCAACCAAT CTGCCGGCAG AGGTGCAGGA TCACACGACG 
CTGGATTATA GCTTCAAAGC GGCGATTGCC AACACCTTTC CAGAGCTACC GCGCGCTCAG 
TGTGGTAGCG TTGTGCGCTG GTATATGATG CTGACCTGCG CCACATCCAC CGCCGAGTCC 
CACAATAGCA TCTCTGAGCA GGCGATCAGT CTGTTAAAAG AGATTGCAGC CGAAATCGAC 
AAGCGTTGGG ACCCCCACTG TGCTCTGTTA TGCACGCGAT TCGGGCTCTA CGGGTATCCA 
TTTGAGCCGG AAATCTTTGA TTTTGACTTC CCCAACGTCA GCAGACAAGG TCCCGGTTCA 
TCGGTTGCCA ATGTGATACG CAGCAGTCCC ACAATTCTGC CAGTGACTGG TCTGGACATC 
AAGCGATTAA GCTCAATAGA TGGCGTAGAC TTCCGCACAT TTCCATATCT GATCCGAGGA 
AAAAGCATCA GTAACCAACT GCGTGGATTG TTAGAGGTGG TTCAGCTTCA CTTTAACTGC 
GTTCAGACTT CCGAAGCTAC TCGTATCGAC AATATTGATA GTGTGGGAGG GAACACTGGT 
GCCAACGTGA TAATCGAGGA TGTTATGATG ATGCCAAAAC AAGTGGTTGC AAAGGAGAAA 
GGAGAGGAAA TGCTCAATGA TTTGGTCAAT GATGTTGAGT GTCTGGTAGA GGAAATGAAG 
GTAGATGAAA ATGAAAAGCA GGAAAAGGTC GATTACGATG CTTTTGGTCT GGTCAATGGC 
TTCAAAGATA AAACATTGTC TTCGTTAAAC TCCGTTAAAC AGACCTTAAA GGAGGAGGTT 
AAAAAAGAAA AAGCAGACAT AATGGACAAA TTAACTATTG CAAAGTTTTT ACCGAAGTTG 
TCGCTACTTG AAAGCTACAT GCAAAAGAAG ATCAATGAGC ACATTTCGTT CGATGACTTT 
GGAAAACTGG ACAACTGGGA CGGTAACACA ACGCCGCCAT TTAATTTGGA GGTGGAAGCT 
CCCATCGTAT GCTCTGAGGC TACGTCCGAA TCAAACATGT CACAACAAGC CACGTCAGCA 
GGTGGTAACA TACTTGACGA TGCCGCGGAT AGCGAAAAGA TCACTGAGAG TCTAAACGCC 
AATCCGTGCC AGCCGATTGC CTGGCACAAA TTAATCACGC CGCCGCCCAA ACAGATGATC 
GTCATCGATC GCATGCATTC GGGTGCACGG CGATTTGTGG TTTTAGACTT TGGACAGCAT 
ATTCTTCTAA CAGATTTGGT GATTCCCTCT TGCGATGAGT TGACCTCCCT TAATATCGAC 
ATCTGGTGTT TTGACGAAGA GACCGATTGC ACGCGGTTGG TGCAGGTGAA TGACATTCAA 
ACCAAAACGC TTGTGCTCAG CGACATTCAG CCGGCCCCAA TATGCCGTTA CCTAAAGTTG 
ACCATTATTG GTCGTATCGG CATGTCGGCC ACCAAGTGCA AAGTGCCGTT GGGACGTTTC 
TTTGGCCACC CAGTAGTGCT GGAGCACGAC GGATATGGTG ACCAGCTTAT GAAGTACATC 
AAGCATCCCA CACATAGCCT TCAATCGCAA ATAAAGTCGC TAAATGCACT GTACGAGGAT 
GTCCACTGCC GTTATAGCTT GGCTAGCTCC AAGTTGATGG ATCTACTGGC TCCCATGAGA 
AATAGTGAAC TGTCCAATGT GGCTCATCTA CAGGCCTTTA TCAACAAGCA ACGTGAAGAG 
GAGCTAAGTG GGCTGGAAAA CAACAATAAG GTGGTTTCAC TGTATGATGA GTGCATGCTT 
CTGCAATATC AGATCAATGT TATACGTAAC GTGGTGCTCC GTTTGGAACG AGCTCTTTCC 
CCTAATTCTC CGACGACAAC GCTTCTTCCG GGACAATCTA TTCCTCTAAC GGAGGATATC 
CTGCGCGCTG CCTCGAGGGA CAAGTTGCGA GTGCTTAGTC TGAGTCTGGT GGAGGTGCTC 
TTGCACTTCT CCATTGAGTA TGGAATCAAG AACATCCTGC CCGTTCATCA GAGATTCAAT 
GCCGCCACGG CGAAGGCGCT TTTCAACGCC CTGGTTGTGT ACGGCGATGC CCAGCTACAG 
TTGGCAACAT GCTCCTTGCT TGTGCGTATG TGCTGCTTCC AACCGTGGTG GGGTGACTTT 
CTCGCTGACA CCTTCTGCAG CCTATTCTCT GCCCAGAACT GCAAGGCTTT CCCCCAGGAC 
CGCATATTCT TTTTGCTCAC CTACCTGGGA CGTCGAAGCA TTGCTATGGG CGCATGCCGA 
TCTATAGTAA TCGATGCCGT GCTTAAGACG CTCGTCAAGC TGCTGGCCCC CATCTCACCG 
CACTACAAAT TCCAGTCGGA GCAGCCCAGC ACGTCGGCAA ACGCTGCTGA CCGCAATGCA 
GCAATGGGCA CCACATCTGA TCTGCAGCTG ATTACCTGGC TACTTCTGTT TCTCTCAGTG 
TGTCTGGATG ACAACGAGCG CAAGGATAAA TCAGCAGCTC GCTGGGACTT CATGAGCTGC 
GAGGGCGATT TTACTAAGGC CAGACCTAGT GCAACTCCCA ACATCAAACA GCTACGATGC 
TTTAAAAAAC GCATCATTCA GCAAAACAAG TACTTGGCGC AGCCTTACGC TGACTGGGGA 
AAGAAGATTT ATATGATACA GAATGAGCAT CCAGGCATTT TCATGGAGCT GTCAACGAAA 
TCAAAGGGCG CTGGACCAAC CAAGTCGACC TCTACAGGTG GCAAGATTAA TATGGGATCC 
TCTGGCCTGT GCGTGGGTGG TCCTTCAAGT TCCAGTGGCA ATACATCAAA GAGTGCGGCT 
TCCTCATCCT CTCCTAAGAA TCAGGCTGTG CCGGGTACCG ATGGCGAACG AGACAGCAAC 
TTTGACAAGG GATTAAAATC ACTTCGAATG GATAACATTA AGGTGGTTAT TCGCGGTCTC 
TTTGCACTGT TGCTTGAAAT GGACTTCGAT TGCAATATGG ACCAGTTTTT GCTCACTTGC 
AAAGTCATTG CCCGTTTGGT GGCTGCATGC AGACCGTCGG TACAGCTGTG TAAGATTGTG 
ACCATCGCCC AACTGCAGCA ACTTGTACGT CTATCCGTGT GGAATGATCA GCAGCATCCC 
TGGGCTGTTC ATGCCATTAC GTGTTTACTG CAAGATCTTT TGGATGCGGA CAAGCAGTTC 
CGGGACAATG ATGTCACACC TACAAATGAC ACGCCGCGTA CAATGGAAGC TGCGATCCAG 
GAAACACGGG ATCTTTTCAG CGACTATACT TCCACTGCTT CCTCGTGTAC TGAGGCACTA 
TTTGAGACGC TGATGCCGAC AGCAAGAGAA TTTTATCAAG AGGCCGTCAA ATATAATTAC 
CTACCTTCAT TAATTGAATG CGATGACACG GAATTCGATG AAATGCTAAA CGACATTGAC 
ATGATTGAAC GAACGAAGCC AGTTACCAAA AAGGAAAGTA ATGCCCTGTC TAAGAATACA 
TTCAATTTCT TCTGCAAGTC TATCTCGATT GCTCTGGATT CACGATTGGA CGTGGGTCTG 
GAATTGCACG TAGAAACTCA GCTGAGAAGG CTAACAAATC GCACATCCCT GGATCTCTAT 
TCATCGCTTC CTCAGGTGTT GACCAATGAG TTTGTCTCTC CACCACCTGA GGCTGCTCCT 
TGGCCAGAAT ATTTGACTCA ACTTTGGTCA GGCACGGAAT ACAACGGTGA CAATACTTAT 
GTGATGTTTA AAAGGGTCTT TGACTCCATC CTGGCGGAAT TGCACCTTGA AGACACGTGG 
GTGCACTTGG AGCAGGTGCT GCAGATGTGG CTGACGCTAA ATTGCGAGCT CTCGGACAAG 
CCATACACCA GTGGCATCAC TCACTCGGAT GTTCCAAAAA TTCCGTTTGG AGCAAATGCG 
GTGCAGGGAT TGCTCCTGGC TTTGGCTTGG CACAGTGATA TAAAACTGCG GACCTGGTGT 
CTTGGCTTTC AGTGCCTGTT TCTCGCTTGC AACTCATTGC CTATCGGAGA TGATGCGGAT 
TGTACGCGTA TCAACGAGGC TATCGTGAAT GATGAAAACT TTGAAAAAGT GCTGCTACGA 
TTCTTTTCCG GTTATGGCAT GAGCTCATCT ATCATCACCA ACCGATGCGC TGGACCTACT 
ATTTGCAAGC ACTTGCATGA GTTATTGCTC TGGCTGCACA GCAAAAGCGA GACGAGTGGC 
ATTCCGTGCA AGCGTCGACT TAAGGACATT TTGTTGCATG TTGTGCTTCA GTTGGTGCAG 
CCTGGTGGCT CTATTAGCAA CCAGCAGGGT CCAATTGATG CTCAAAACCA GCTGGTTCGT 
GATTTGCTTA TGATGCCCAA TGATAAGGGG GACCTCAACA TTGCGCTTAA AATCACAGAA 
AGTGTTTCTT TCCTGGTGTA CAACAACATA TCCAATGGCG AGAGGCTACA ATGTCAGCGT 
GGAAATGAAA ACAGCCCAGT TGGCAACAAT TTTAGCAATT TGTTTGCCAA CGTTCTGGGA 
TCAGAAAATC CCAACAAGCA GAATGCCGCA ATTTGCGACA ACTCACTTAT TATAAATCTG 
CTGAAACTAT CGTCACACAT GGTATTGACA GAGATGCCCC GGCAACCAAG TGAGGTTACC 
ATTCCCGAGG AGGAGGAACT TTCCAATCAG AGTCAAACGG ATGAGACTAA AGCGGAGCAG 
TTAAACACGG AAGGACATCG CAGCAAAGTG CCTTGCATTG CAGATTGGGT ACTCCGCCAT 
TTCCCCACAA TGAAGCGACT TTTTGGAACG CTTTCCCAGT GCTCGACAAA CACCTTCACC 
ATGATGTCCT CTGGCTGTTT CTTTTCACCG AAGTCCAATA TGATTTTAGA TGACCCGCAG 
ACAATTGCAG ACGCCGTCTT TAGTTTGATG ATTACTCTCA ACGACAAGGC TTCGCATCCC 
CGACTTGTTG TGGTGCCCAT TTGCCGATAC CTTGAAGAAA CCACTATTCC GAGAAATGCA 
ACGCTGCCGC GTCTGCCGCA TTCTGAACCT TTCCTGTGGT ACATCTCGAA GGTATTGGAA 
GTACCAGCGG CAGTGCAAAC GTTCACCCAA TTGGGCGGAA TTCAGATTAT ATGTCATAGC 
CTGGTGCGAC TTAATAAAAC TATCATCAAC ATGCAACCAG GATTGATTTC TTTGATTATG 
CAACACTTGA CGCGCAATAC GCGCTTTAAG CGTAATAATC ATGTGGCGGC AAGTAGTGCT 
TGCAAAAAGA CAACGACTGG TACGGGAACC ACATGTCCCA CGTCAGCACA GCCGCCGAGA 
AGTGCTGCTC AGTACGATGG TCTGATCAAC TTTGCACCCT TCTCGCATAT TGTCTCCGAA 
AATGATGCGA CACAAAGTGC AGAGGTACTA ATATCAAATC CAAATAATTC ACATCGAAGA 
CCGCGATCGC CAGCCTGGAC CTATATGTTC TATCCAAATG AGACGCATGT GGATCTGACT 
ATAACGTTGC CCACAGCAAT TTTGTTGAAA GAGGTGCAGC TGGTGCCGCA CACAACTAGT 
TTGGCATCCT GTCCATCTGC GGTGGCTCTA GAGCTGTCTA GAGATTACGG CCTTGGTTCG 
ATCCCAGTGG GTGCTCCCAT GGCCACCACT GGTCTGACCT GCATTAGACT AAAGCTGGCC 
AAGGCAGAGG TGGCTACCAG CATTGTTCTG AGGCTGTACA AACCCAAGGA CTGTGGAAAT 
GTGGGTCTCG TACAAATCGC TGTGCTTGGT CAGACAATCT TCGGAAATCG CATGCATGGC 
CTTCGCTCAC CGAGTCCGTT CATTGACTCC CTAGCAACGT GCTCCTCGCC AGTGCAAACG 
GCAGTTTTAG CCGCAATGGA TGCGGATTCC ATGCTTGAAG ATGATGCTTT TGCGAAGACT 
AGCATTGGCT GGGTTCGCAT TCTGTCGCGC TGTTTCCATG TGGCCGCTTT ACAACCAGAC 
AGTAAGCTGG CTGATCTTAT CGCCGCCTAT CCGGCTGCAT ATCCCGGCTT TTTAGAGGCC 
TGCTGCTCAT TGCTAAACAT AATGCCAATG ATTCCTAGTC TTGCACAGCA ACACTTGGAG 
ACCATTTTGT TAAAATTAAG TGCATACAAC CACGAGATCA GTTTGCAACT TCTTAGGACG 
CTCCTGTGGC ACACCACTCC GCAAGTTTTT AAACTCTCCA GCGATTCGGT GTGTGATCTT 
ATTTATGAAG TAGTTACCGG ATCGACGGAG CACCTACTGG ACAAACTACG CGTGCTTATC 
GACTGGGTAA TGCAATTGCA CGACAACTAC AGCAAGCAGG CGCGCTGTAA TAACCCACAA 
AGTGGATTTG TTAAGGTGAT TGCTTCGATT CTGTGGAAAG TGCATACCCA GCAACAATTG 
CCTGAGTTAC CGCTTCTCAT TTCATCTTCG CTGTTCGAGA CCTGTTTTGA ATGGGTCTCT 
TCGCTGGAGC ACGATGAGCC ACTGAAGAGC AGTTTTGACT CCTTGTTGTG TGCATTGTGC 
TTCATTAAGC CAGAACTGTT TAACCAGCTG TTGGTCAAGC TGGGAGTTAA GCTGGAACCT 
CCAGCTCGAG GTCAAACTGA TGACAGCAAA GTGCAAAGTG GTAGTGCTTG GTTCCGCCGT 
GAGGGTAGCG AAAACCTTAG TGTTTTGCTA CAGCGTCCCA CATTCCTAAA GACTCTAGCC 
TTGGCCTGCC AGTCCCCTGC AGCAGTTTAC CAGATGCTAG ACTCCGGACT TCCAAAGCTA 
TTGGCCCACG CCATTTACGA GTATTGCATG CATCTCATGC CGGGATCGGA GCCAGTAGCT 
TCTGCATCTA CAGTGCCGGA AGACGAACCG TTAGCCAACA ATCAGGCAGC CGCTGCAGCA 
GATGACGAGG ACTCGTCTCT TACGGACTTT GACAAGGCCG AGGCAAATGT GGTTTATAAT 
ACTCCACTGC TAAGTAGCTC GAATGTACCC AAAGTGCTAG ATTTCTTTGC CGAATGTTGT 
GCCGAAGGTC CAATGCGTGA TTGGTTAGGT ACTATGCAGG GCTCCGTATT TTGGAAGCCT 
CTGCTGCAGT TGCTCTGTAA CACACATGCC GCCCAGTTCA GCAAGACGTT GCCTAAGGAA 
CAATCGTTTA TCCGCCTGGA GAGGGCCACA ATCAACTTCT TTACGCGAGT TAGCGCCTGC 
CATCCCGGAA ACCAGGAAAA TCTTACATCG TTGCTCATTG GAGCCATAAT TTGCAAACCA 
TTAAGATTTT CGCATGGAGT TCGACCAACT ATATCTGGAT TTACTAGACA GATGGTGCTG 
CAGCTCCTCC TCGAGAATGA ACACATCACA GTCTCTGTTA GAAGTCAGCA GCCCTTGCAA 
CGGAGGGATA CCCTCTCCGC CGTTCTAGGC GGGATTTCGA GTGGTGCAAG TACTAGCTTA 
CCCGTAGCAG CAGTCAACAA TCATCCGGCC AGGAGGAGCA GCGCCCACCA CATGCTATTC 
ACGGTGACCA CTAGCACCAC GTGCCAAGAG ATTATGCAGA ATTGCGTTAG TGTGTTTAGT 
AATCTAGTTT ATCAACAACC AACAGATCAG CGAGCTGGCG AGACCTCTGC CTCGAGCAGC 
GGCGTATCGA GTGCAACTGC CAAATCCAGT GAGAGCAAAA TGGAAAAGAG CAGCTTCCTG 
AACTTTAACA ACGTAGAAGC GGGTAGCATG GAGTTCCTTA CCGTTGGTGC TGCTGTGGCA 
GCCAAGGACA AGCGCATAAA AGATGCTAAG AACCAAGCAG CCATGAACAA GGACAAGGAG 
ACTCAGACCA GCTCAACGAA CTTGGCTTCC AATATATTCA ATGTGGATGA GTTCTTGTTG 
CAATCGGCGA ATGCCGTTAA TGGTAACCAG TTGGTCATTC CAGAGTATTC TGATATTGTT 
CTAGCTGGAG ATGCCACCAT CTCGCAGGTT TTGGCCTCGC TTCAGAATGC AGGACATTCT 
CTTTCGACGC CCTGTATATC GTTTGATCTG GTACTTCAAC AGCGCTCTGA TGACACAAGC 
GAGGCAAAGA AGACCAAAGC TGCCTGCACT GAGCCGTTGC CATCACCACT GCAACGATTT 
TCCAGCAGTG GTGGTCTATC TTTACTCGCT CACTACCTGC CCACCGTATA TCCGGAACAC 
TCAGGTCGCA AGACCACCTT GGTGTTTAGC GAGAAGGAGA AAAGTCCCCC GCTCTCTGAC 
TGGGTGAAAT TGGAACCGAA CGACGAGATC TATGAGGATT TGGAGGACAC TCTCTGTGAG 
CCAGCAGCTA AGCTGGCAAC GGTCAGTTCG GTGCCGCAGC ACTCGCTGGC CGCATTTGGA 
TTATTCCTGC GCCTGCCCGC CTACTCTGAT GTCTTGCTGC GAGACAAGAT GCGGGCGCAG 
TGTCTTCTCC GCTTAGTGCT CGGAGTCACG GGAGATGGGG ATGGCAATGA TATATACAGT 
CTGTCGCTGG CACCCTCCTT GCCTACATTA CCCTTCGAGG TCTTCCGTCA GCTTCTTGAT 
AGTGTGCCGT TATCCACCGA CGACGGTGTT TTGCTACGAC GCGTGGTAAT CGAAGTGGGG 
GCCATGCACC TAGTGCTTAA TTGCCTCGGC ATTTTCTCGC ATCAGTCGCA CAACAACAGG 
ATTGGAGCAC CGATTAGCGA ACCATCGCCA AGTGGAACCA GTAGTGGTAG CGGCACCATT 
GGCAATGGAA GTGGAAACGG AAGTGTAAGC GGTACCAAAA CTAATGCACC AGAAGAGGCC 
ACACCTAGTG CCACGTCGGA CGACAAAAGC CACATGTACT GGGCCAAGGG CACTGGTTTC 
GGTACCGGTT CTACTACTCA GTCGTGGAAT GTGGAGCAGG CGCTTCTTCG GCAAAAGAGC 
GAGGAGGAGC ATGTCACAGT GTTGCTGCTT GTTCTAGCCA GTTACATAAA TCCGGGTGAC 
TGTATCCCAA GTGAAATGTG CGGAGAGGAC ATCCTAGCTT ATCATGATGT CCGAGATGTT 
ACCGGCGAGT TGCCGCCCAT CTTCCAAACC CTACTGCAGC AGTCTTGCTT AATCCCCGCC 
CTCAGCTCCT ATCTGCGCAA CGATTCAGTG CTTGATATCA CGCGCCACAT TCCGCTATAC 
CGTGCCATAC TCAAACTTCT GCGTGCCCTA TCGCTGTCTA AGATGCTTGT CTCGTTGCTC 
CAACCATCTG CCAGTGGCAG TGGGGACAGC ACGCCACCCA TAGTGGAGTT GCTCACAAGC 
ATGAAGACCT GTGTGGATAC CTACGCCAAA CGGTTAAAAG TTAACAAGAA GTCAAACATA 
AAGGGACAGA CACATCAGTT GACCTTTAAC ATCGATGATG GCGACGATGA GGGATTAGCT 
TTGCTTATTC CGGATATCCA TGAAACCAGT GTGCTGGTGC AAAAAACCAC AGATGCTGAT 
GCGCTAATCA ACATGTTGCA TACCAACGAA GACGGCAGCG CACAGCTAGA GCCCCAGAGC 
AAATCAATTG AACAGCGCTA CTTAGAGCTA ATGAAGAAGC GCCAGTTCGA TACGTTTGAC 
ATGATTGTGG AGTCGGATAA CAACAGTTTC CGCTTTGTGG TCTCGCATCA CTTCGAAAAA 
ATGGTTCGGC TCGCTGGCGA TCGTTACCAT CCGTCGCGGG TCAAGCGACT TGCCCAGGAG 
GCAGTTACAC TGTCCACCAG CCTACCCCTC AGCTTTAGTA GTTCTGTATT TGTACGCTGT 
GATACTGATA GATTGGACAT CATGAAGGTA CTCATAACTG GTCCGGCGGA CACGCCTTAC 
GCCAACGGGT GCTTTGAGTT TGATGTCTTC TTTCCGCCGG ACTACCCTAA CCAGCCCATG 
CTCATCAATC TGGAGACGAC GGGTCGCCAT TCGGTGCGCT TTAACCCAAA CCTCTATAAT 
GACGGCAAAG TTTGCCTGTC AGTTCTCAAC ACCTGGCATG GTCGGCCTGA GGAGAAATGG 
AATGCACAGA CTTCAAGCTT CCTGCAGGTA CTCGTTTCCA TCCAGTCCCT GATCCTCGTT 
CCCGAGCCCT ATTTCAATGA ACCGGGCTTT GAAAGAAGTC GTGGCAGTCC AAGTGGTACC 
AATTCATCGC GAGAATATAA TAGCAATATA TATCAGGCCT GTGTGCGGTG GGCTATGTTG 
GAGCAAATAC GGAGTCCAAG TCAGTGCTTT AAAGATGTCA TACACAAGCA CTTCTGGTTG 
AAGAGAGAGG AGATTTGCGC CCAGATAGAG GGCTGGATCG AGGAACTCGG TAAGCCGCAG 
TACACCGAGA GGGCCAGTCG CACTATATCG TTCAACTCAA TGGTCCTGCG CCGCCACTAT 
CGCCACCTGC GTGAGGAGTT GTCGAAACTG AAGCCGCCAC GTGGCCTCGA GGACTTGGAT 
GCGCCCTTTA ATCCTGTGGC CACGCTACCA CCGATGGATG TGTCCGCCGT GGCTCCTTCA 
CTAGCAGCCA CAAATACCGT CCAAGCGCAA CCGGATGATG CCATAGTGAC CGATATTACC 
CTGCTCGGCG AGAATGCGGA TGAATGCGAA GCTGATGGCG ATGCCGAAGG CGATGGAGAA 
GTGGACGATG GCTTTAAGGG TTTCCCGCAA GAAGAGGGTT TGCTGACGGG CGAGGAAGAG 
GTCGTGGAAA TTTTAAGTGA TACGGAGAAC GAGAGCAGTA TGTGGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Bruce cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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