Deaf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Deaf1
Entrez Gene ID	40164
Full Name	Deformed epidermal autoregulatory factor-1
Synonyms	CG8567,DEAF-1,DEAF1,Deaf-1,Dmel\CG8567,deaf1,l(3)76BDv
General protein information	Preferred Names Deformed epidermal autoregulatory factor-1 Names CG8567 gene product from transcript CG8567-RD CG8567-PA CG8567-PB CG8567-PC CG8567-PD CG8567-PE Deaf1-PA Deaf1-PB Deaf1-PC Deaf1-PD Deaf1-PE deaf-1 deformed epidermal autoregulatory factor-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76D1-76D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275141.1	NP_001262070.1	deformed epidermal autoregulatory factor-1, isoform E
NM_001275140.1	NP_001262069.1	deformed epidermal autoregulatory factor-1, isoform D
NM_079445.3	NP_524169.1	deformed epidermal autoregulatory factor-1, isoform A
NM_001038944.2	NP_001034033.1	deformed epidermal autoregulatory factor-1, isoform B
NM_001275139.1	NP_001262068.1	deformed epidermal autoregulatory factor-1, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Deaf1	NP_001262068.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004905	XP_314996.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DEAF-1 is a regulator of Drosophila immunity; it activates the expression of Mtk and Drs promoter.
Title: DEAF-1 regulates immunity gene expression in Drosophila.

effects of loss or gain of DEAF-1 function on Drosophila development
Title: DEAF-1 function is essential for the early embryonic development of Drosophila.

The following Deaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Deaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19019	NM_001275140.1 Latest version!	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant D (Deaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19019	NM_079445.3 Latest version!	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant A (Deaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19020	NM_001038944.2 Latest version!	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant B (Deaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21100	NM_001275139.1 Latest version!	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant C (Deaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21101	NM_001275141.1 Latest version!	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant E (Deaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Deaf1 ( NM_001275140.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262069.1 Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant D (Deaf1), mRNA.

Price & Availability↑

CloneID	OFa19019
Clone ID Related Accession (Same CDS sequence)	NM_001275140.1 , NM_079445.3
Accession Version	NM_001275140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deformed epidermal autoregulatory factor-1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACAGG TTGACTCCTC CACCGAGCTG CACCTGAATA GGAAAGATCT GGCCGCGCTG 
GCCGAGGATG TTGTGAAAGA AGAGGTGATA TTAGAAAGCT CCAGCCATCA CCATCATCAT 
CATCACCACC AGTTGGACAC CAAGGTTCGC ATGGTCACAT CCTCCTCCAA CGACAACAGT 
GGTAGCGGCG GCGCCAGTGG CGGTACAAGC GGAGCGGGCG GAGGAAACGG CGGTGGCGGT 
GTGGTTTCGG TGCCCGTATC CCTGCCCATC GGTTCGATGA TAACCGGCAC TACCTTCAAT 
GTGATCACGC CCGACCAGCT GCCCCCTCAC TTCAAGCCGA TGCTGTGCGT CGACAACAAT 
GGCTACCTGT CCGGCAGCAC GGTCAGCATG GGCAACGACC TCAAGACGAT CGTCATCCAG 
CAACAGCAGA CGCAACCAGG CGGCGGCGGT GGCGGCGCCA ATAATGCGGG CACCAATACG 
ACCGCCACAA ATACGATTGG ACTGAATCAC GATGGCTCCG GGAGCAACAA TAGCCACGAC 
AGCCTGGCCA CCTTGGAGCA CGCCGCGGGC GGAGCGAGTG GCGTCGGCGG TGGCGGCGGC 
GGAACGGGTG GTGGCTCCTC GGGATGGTCG GAGAACCCGT CAACCCAGCA CAATGAGGTC 
TTCCAGATCC GCTGCAAAAC CACCTGCGCC GAGTTATACC GCAGCAAGCT GGGCTCCGGC 
GGCCGTGGAC GCTGTGTGAA GTACAAGGAC AAGTGGCATA CGCCCAGTGA GTTTGAGCAT 
GTGTGTGGCC GGGGCTCCAG CAAGGATTGG AAGCGTTCGA TCAAGTACGG CGGCAAATCG 
CTGCAGTCCC TCATCGATGA GGGTACGCTC ACGCCGCACG CGACCAACTG CAGCTGCACC 
GTCTGCTGCG ACGACGAAGC AGGTGAGTCA GCTTCCGGCC CTGTCCGTCT CTTTACACCG 
TACAAGCGGC GGAAGCGGAA TCAAACTGAT CTCGATATGG AGTCTGGACC CAAACGCAAG 
CGTAACACCC ATCATAGTAA CAACAACAAC AGTAACACCA ATAACAACAA CACGAGCGGG 
AGCGGCGCCA ACAACTGTGT GGATGTAACT GCAGCTGTGG CTGCTGCAAC AGCCAGCGTG 
GTGGACGAGA ACAACATGTT CCTGTCGGAA GAGAACATCA CGTCCAAGGA TGAGCCGTGG 
GCAGCGCTCA ACGACAGCCT GGACACCTCC ACCGAGCTGG TCGATCAGTC GCAGATGGGC 
AACACGTACG AACGGGAAAC GTTTGTGGTG AACATCAACG ATGGATCCTC CATCGCCGTC 
CTGGACACCA GCCAGTCGAT GAAGAACATC GAGCACGTGT ACTGCACCAT GGTCAAGGCC 
ACCAATGACT TCAAACGCAT GCTTAACGAC ATGAAGCAGT CCTTCGAGCG TCGCATCGAG 
GTTCTGCAAA AGGAGCGCGA TGCAGCGGTC AGCGCGATGC GTGTCCAAGT GCACGCGGAC 
ATCGATGATC CAAATATTTC AGGCTCCCTG CACGGCAACG AGATCATTTC GGCTAAGAAG 
TGCGCCAACT GTAACCGCGA AGCATTGGCC GAGTGCTCAC TGTGCCGAAA GACGCCGTAC 
TGCTCGGAGT TCTGCCAGCG GAAGGATTGG AATGCTCACC AGGTGGAGTG CACAAGGAAT 
CCACAAACGA CAACGCAGCA GGTCATGCTG TTGATCGACG ATCAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262069.1
CDS	362..2092
Translation	MEQVDSSTELHLNRKDLAALAEDVVKEEVILESSSHHHHHHHHQLDTKVRMVTSSSNDNSGSGGASGGTSGAGGGNGGGGVVSVPVSLPIGSMITGTTFNVITPDQLPPHFKPMLCVDNNGYLSGSTVSMGNDLKTIVIQQQQTQPGGGGGGANNAGTNTTATNTIGLNHDGSGSNNSHDSLATLEHAAGGASGVGGGGGGTGGGSSGWSENPSTQHNEVFQIRCKTTCAELYRSKLGSGGRGRCVKYKDKWHTPSEFEHVCGRGSSKDWKRSIKYGGKSLQSLIDEGTLTPHATNCSCTVCCDDEAGESASGPVRLFTPYKRRKRNQTDLDMESGPKRKRNTHHSNNNNSNTNNNNTSGSGANNCVDVTAAVAAATASVVDENNMFLSEENITSKDEPWAALNDSLDTSTELVDQSQMGNTYERETFVVNINDGSSIAVLDTSQSMKNIEHVYCTMVKATNDFKRMLNDMKQSFERRIEVLQKERDAAVSAMRVQVHADIDDPNISGSLHGNEIISAKKCANCNREALAECSLCRKTPYCSEFCQRKDWNAHQVECTRNPQTTTQQVMLLIDDQS

Target ORF information:

RefSeq Version	NM_001275140.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant D (Deaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275140.1

ORF Insert Sequence:

ATGGAACAGG TTGACTCCTC CACCGAGCTG CACCTGAATA GGAAAGATCT GGCCGCGCTG 
GCCGAGGATG TTGTGAAAGA AGAGGTGATA TTAGAAAGCT CCAGCCATCA CCATCATCAT 
CATCACCACC AGTTGGACAC CAAGGTTCGC ATGGTCACAT CCTCCTCCAA CGACAACAGT 
GGTAGCGGCG GCGCCAGTGG CGGTACAAGC GGAGCGGGCG GAGGAAACGG CGGTGGCGGT 
GTGGTTTCGG TGCCCGTATC CCTGCCCATC GGTTCGATGA TAACCGGCAC TACCTTCAAT 
GTGATCACGC CCGACCAGCT GCCCCCTCAC TTCAAGCCGA TGCTGTGCGT CGACAACAAT 
GGCTACCTGT CCGGCAGCAC GGTCAGCATG GGCAACGACC TCAAGACGAT CGTCATCCAG 
CAACAGCAGA CGCAACCAGG CGGCGGCGGT GGCGGCGCCA ATAATGCGGG CACCAATACG 
ACCGCCACAA ATACGATTGG ACTGAATCAC GATGGCTCCG GGAGCAACAA TAGCCACGAC 
AGCCTGGCCA CCTTGGAGCA CGCCGCGGGC GGAGCGAGTG GCGTCGGCGG TGGCGGCGGC 
GGAACGGGTG GTGGCTCCTC GGGATGGTCG GAGAACCCGT CAACCCAGCA CAATGAGGTC 
TTCCAGATCC GCTGCAAAAC CACCTGCGCC GAGTTATACC GCAGCAAGCT GGGCTCCGGC 
GGCCGTGGAC GCTGTGTGAA GTACAAGGAC AAGTGGCATA CGCCCAGTGA GTTTGAGCAT 
GTGTGTGGCC GGGGCTCCAG CAAGGATTGG AAGCGTTCGA TCAAGTACGG CGGCAAATCG 
CTGCAGTCCC TCATCGATGA GGGTACGCTC ACGCCGCACG CGACCAACTG CAGCTGCACC 
GTCTGCTGCG ACGACGAAGC AGGTGAGTCA GCTTCCGGCC CTGTCCGTCT CTTTACACCG 
TACAAGCGGC GGAAGCGGAA TCAAACTGAT CTCGATATGG AGTCTGGACC CAAACGCAAG 
CGTAACACCC ATCATAGTAA CAACAACAAC AGTAACACCA ATAACAACAA CACGAGCGGG 
AGCGGCGCCA ACAACTGTGT GGATGTAACT GCAGCTGTGG CTGCTGCAAC AGCCAGCGTG 
GTGGACGAGA ACAACATGTT CCTGTCGGAA GAGAACATCA CGTCCAAGGA TGAGCCGTGG 
GCAGCGCTCA ACGACAGCCT GGACACCTCC ACCGAGCTGG TCGATCAGTC GCAGATGGGC 
AACACGTACG AACGGGAAAC GTTTGTGGTG AACATCAACG ATGGATCCTC CATCGCCGTC 
CTGGACACCA GCCAGTCGAT GAAGAACATC GAGCACGTGT ACTGCACCAT GGTCAAGGCC 
ACCAATGACT TCAAACGCAT GCTTAACGAC ATGAAGCAGT CCTTCGAGCG TCGCATCGAG 
GTTCTGCAAA AGGAGCGCGA TGCAGCGGTC AGCGCGATGC GTGTCCAAGT GCACGCGGAC 
ATCGATGATC CAAATATTTC AGGCTCCCTG CACGGCAACG AGATCATTTC GGCTAAGAAG 
TGCGCCAACT GTAACCGCGA AGCATTGGCC GAGTGCTCAC TGTGCCGAAA GACGCCGTAC 
TGCTCGGAGT TCTGCCAGCG GAAGGATTGG AATGCTCACC AGGTGGAGTG CACAAGGAAT 
CCACAAACGA CAACGCAGCA GGTCATGCTG TTGATCGACG ATCAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( NM_079445.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524169.1 Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant A (Deaf1), mRNA.

Price & Availability↑

CloneID	OFa19019
Clone ID Related Accession (Same CDS sequence)	NM_001275140.1 , NM_079445.3
Accession Version	NM_079445.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deformed epidermal autoregulatory factor-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079445.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACAGG TTGACTCCTC CACCGAGCTG CACCTGAATA GGAAAGATCT GGCCGCGCTG 
GCCGAGGATG TTGTGAAAGA AGAGGTGATA TTAGAAAGCT CCAGCCATCA CCATCATCAT 
CATCACCACC AGTTGGACAC CAAGGTTCGC ATGGTCACAT CCTCCTCCAA CGACAACAGT 
GGTAGCGGCG GCGCCAGTGG CGGTACAAGC GGAGCGGGCG GAGGAAACGG CGGTGGCGGT 
GTGGTTTCGG TGCCCGTATC CCTGCCCATC GGTTCGATGA TAACCGGCAC TACCTTCAAT 
GTGATCACGC CCGACCAGCT GCCCCCTCAC TTCAAGCCGA TGCTGTGCGT CGACAACAAT 
GGCTACCTGT CCGGCAGCAC GGTCAGCATG GGCAACGACC TCAAGACGAT CGTCATCCAG 
CAACAGCAGA CGCAACCAGG CGGCGGCGGT GGCGGCGCCA ATAATGCGGG CACCAATACG 
ACCGCCACAA ATACGATTGG ACTGAATCAC GATGGCTCCG GGAGCAACAA TAGCCACGAC 
AGCCTGGCCA CCTTGGAGCA CGCCGCGGGC GGAGCGAGTG GCGTCGGCGG TGGCGGCGGC 
GGAACGGGTG GTGGCTCCTC GGGATGGTCG GAGAACCCGT CAACCCAGCA CAATGAGGTC 
TTCCAGATCC GCTGCAAAAC CACCTGCGCC GAGTTATACC GCAGCAAGCT GGGCTCCGGC 
GGCCGTGGAC GCTGTGTGAA GTACAAGGAC AAGTGGCATA CGCCCAGTGA GTTTGAGCAT 
GTGTGTGGCC GGGGCTCCAG CAAGGATTGG AAGCGTTCGA TCAAGTACGG CGGCAAATCG 
CTGCAGTCCC TCATCGATGA GGGTACGCTC ACGCCGCACG CGACCAACTG CAGCTGCACC 
GTCTGCTGCG ACGACGAAGC AGGTGAGTCA GCTTCCGGCC CTGTCCGTCT CTTTACACCG 
TACAAGCGGC GGAAGCGGAA TCAAACTGAT CTCGATATGG AGTCTGGACC CAAACGCAAG 
CGTAACACCC ATCATAGTAA CAACAACAAC AGTAACACCA ATAACAACAA CACGAGCGGG 
AGCGGCGCCA ACAACTGTGT GGATGTAACT GCAGCTGTGG CTGCTGCAAC AGCCAGCGTG 
GTGGACGAGA ACAACATGTT CCTGTCGGAA GAGAACATCA CGTCCAAGGA TGAGCCGTGG 
GCAGCGCTCA ACGACAGCCT GGACACCTCC ACCGAGCTGG TCGATCAGTC GCAGATGGGC 
AACACGTACG AACGGGAAAC GTTTGTGGTG AACATCAACG ATGGATCCTC CATCGCCGTC 
CTGGACACCA GCCAGTCGAT GAAGAACATC GAGCACGTGT ACTGCACCAT GGTCAAGGCC 
ACCAATGACT TCAAACGCAT GCTTAACGAC ATGAAGCAGT CCTTCGAGCG TCGCATCGAG 
GTTCTGCAAA AGGAGCGCGA TGCAGCGGTC AGCGCGATGC GTGTCCAAGT GCACGCGGAC 
ATCGATGATC CAAATATTTC AGGCTCCCTG CACGGCAACG AGATCATTTC GGCTAAGAAG 
TGCGCCAACT GTAACCGCGA AGCATTGGCC GAGTGCTCAC TGTGCCGAAA GACGCCGTAC 
TGCTCGGAGT TCTGCCAGCG GAAGGATTGG AATGCTCACC AGGTGGAGTG CACAAGGAAT 
CCACAAACGA CAACGCAGCA GGTCATGCTG TTGATCGACG ATCAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524169.1
CDS	362..2092
Translation	MEQVDSSTELHLNRKDLAALAEDVVKEEVILESSSHHHHHHHHQLDTKVRMVTSSSNDNSGSGGASGGTSGAGGGNGGGGVVSVPVSLPIGSMITGTTFNVITPDQLPPHFKPMLCVDNNGYLSGSTVSMGNDLKTIVIQQQQTQPGGGGGGANNAGTNTTATNTIGLNHDGSGSNNSHDSLATLEHAAGGASGVGGGGGGTGGGSSGWSENPSTQHNEVFQIRCKTTCAELYRSKLGSGGRGRCVKYKDKWHTPSEFEHVCGRGSSKDWKRSIKYGGKSLQSLIDEGTLTPHATNCSCTVCCDDEAGESASGPVRLFTPYKRRKRNQTDLDMESGPKRKRNTHHSNNNNSNTNNNNTSGSGANNCVDVTAAVAAATASVVDENNMFLSEENITSKDEPWAALNDSLDTSTELVDQSQMGNTYERETFVVNINDGSSIAVLDTSQSMKNIEHVYCTMVKATNDFKRMLNDMKQSFERRIEVLQKERDAAVSAMRVQVHADIDDPNISGSLHGNEIISAKKCANCNREALAECSLCRKTPYCSEFCQRKDWNAHQVECTRNPQTTTQQVMLLIDDQS

Target ORF information:

RefSeq Version	NM_079445.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant A (Deaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079445.3

ORF Insert Sequence:

ATGGAACAGG TTGACTCCTC CACCGAGCTG CACCTGAATA GGAAAGATCT GGCCGCGCTG 
GCCGAGGATG TTGTGAAAGA AGAGGTGATA TTAGAAAGCT CCAGCCATCA CCATCATCAT 
CATCACCACC AGTTGGACAC CAAGGTTCGC ATGGTCACAT CCTCCTCCAA CGACAACAGT 
GGTAGCGGCG GCGCCAGTGG CGGTACAAGC GGAGCGGGCG GAGGAAACGG CGGTGGCGGT 
GTGGTTTCGG TGCCCGTATC CCTGCCCATC GGTTCGATGA TAACCGGCAC TACCTTCAAT 
GTGATCACGC CCGACCAGCT GCCCCCTCAC TTCAAGCCGA TGCTGTGCGT CGACAACAAT 
GGCTACCTGT CCGGCAGCAC GGTCAGCATG GGCAACGACC TCAAGACGAT CGTCATCCAG 
CAACAGCAGA CGCAACCAGG CGGCGGCGGT GGCGGCGCCA ATAATGCGGG CACCAATACG 
ACCGCCACAA ATACGATTGG ACTGAATCAC GATGGCTCCG GGAGCAACAA TAGCCACGAC 
AGCCTGGCCA CCTTGGAGCA CGCCGCGGGC GGAGCGAGTG GCGTCGGCGG TGGCGGCGGC 
GGAACGGGTG GTGGCTCCTC GGGATGGTCG GAGAACCCGT CAACCCAGCA CAATGAGGTC 
TTCCAGATCC GCTGCAAAAC CACCTGCGCC GAGTTATACC GCAGCAAGCT GGGCTCCGGC 
GGCCGTGGAC GCTGTGTGAA GTACAAGGAC AAGTGGCATA CGCCCAGTGA GTTTGAGCAT 
GTGTGTGGCC GGGGCTCCAG CAAGGATTGG AAGCGTTCGA TCAAGTACGG CGGCAAATCG 
CTGCAGTCCC TCATCGATGA GGGTACGCTC ACGCCGCACG CGACCAACTG CAGCTGCACC 
GTCTGCTGCG ACGACGAAGC AGGTGAGTCA GCTTCCGGCC CTGTCCGTCT CTTTACACCG 
TACAAGCGGC GGAAGCGGAA TCAAACTGAT CTCGATATGG AGTCTGGACC CAAACGCAAG 
CGTAACACCC ATCATAGTAA CAACAACAAC AGTAACACCA ATAACAACAA CACGAGCGGG 
AGCGGCGCCA ACAACTGTGT GGATGTAACT GCAGCTGTGG CTGCTGCAAC AGCCAGCGTG 
GTGGACGAGA ACAACATGTT CCTGTCGGAA GAGAACATCA CGTCCAAGGA TGAGCCGTGG 
GCAGCGCTCA ACGACAGCCT GGACACCTCC ACCGAGCTGG TCGATCAGTC GCAGATGGGC 
AACACGTACG AACGGGAAAC GTTTGTGGTG AACATCAACG ATGGATCCTC CATCGCCGTC 
CTGGACACCA GCCAGTCGAT GAAGAACATC GAGCACGTGT ACTGCACCAT GGTCAAGGCC 
ACCAATGACT TCAAACGCAT GCTTAACGAC ATGAAGCAGT CCTTCGAGCG TCGCATCGAG 
GTTCTGCAAA AGGAGCGCGA TGCAGCGGTC AGCGCGATGC GTGTCCAAGT GCACGCGGAC 
ATCGATGATC CAAATATTTC AGGCTCCCTG CACGGCAACG AGATCATTTC GGCTAAGAAG 
TGCGCCAACT GTAACCGCGA AGCATTGGCC GAGTGCTCAC TGTGCCGAAA GACGCCGTAC 
TGCTCGGAGT TCTGCCAGCG GAAGGATTGG AATGCTCACC AGGTGGAGTG CACAAGGAAT 
CCACAAACGA CAACGCAGCA GGTCATGCTG TTGATCGACG ATCAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( NM_001038944.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001034033.1 Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant B (Deaf1), mRNA.

Price & Availability↑

CloneID	OFa19020
Clone ID Related Accession (Same CDS sequence)	NM_001038944.2
Accession Version	NM_001038944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deformed epidermal autoregulatory factor-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001038944.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACAGG TTGACTCCTC CACCGAGCTG CACCTGAATA GGAAAGATCT GGCCGCGCTG 
GCCGAGGATG TTGTGAAAGA AGAGGTGATA TTAGAAAGCT CCAGCCATCA CCATCATCAT 
CATCACCACC AGTTGGACAC CAAGGTTCGC ATGGTCACAT CCTCCTCCAA CGACAACAGT 
GGTAGCGGCG GCGCCAGTGG CGGTACAAGC GGAGCGGGCG GAGGAAACGG CGGTGGCGGT 
GTGGTTTCGG TGCCCGTATC CCTGCCCATC GGTTCGATGA TAACCGGCAC TACCTTCAAT 
GTGATCACGC CCGACCAGCT GCCCCCTCAC TTCAAGCCGA TGCTGTGCGT CGACAACAAT 
GGCTACCTGT CCGGCAGCAC GGTCAGCATG GGCAACGACC TCAAGACGAT CGTCATCCAG 
CAACAGCAGA CGCAACCAGG CGGCGGCGGT GGCGGCGCCA ATAATGCGGG CACCAATACG 
ACCGCCACAA ATACGATTGG ACTGAATCAC GATGGCTCCG GGAGCAACAA TAGCCACGAC 
AGCCTGGCCA CCTTGGAGCA CGCCGCGGGC GGAGCGAGTG GCGTCGGCGG TGGCGGCGGC 
GGAACGGGTG GTGGCTCCTC GGGATGGTCG GAGAACCCGT CAACCCAGCA CAATGAGGTC 
TTCCAGATCC GCTGCAAAAC CACCTGCGCC GAGTTATACC GCAGCAAGCT GGGCTCCGGC 
GGCCGTGGAC GCTGTGTGAA GTACAAGGAC AAGTGGCATA CGCCCAGTGA GTTTGAGCAT 
GTGTGTGGCC GGGGCTCCAG CAAGGATTGG AAGCGTTCGA TCAAGTACGG CGGCAAATCG 
CTGCAGTCCC TCATCGATGA GGGTACGCTC ACGCCGCACG CGACCAACTG CAGCTGCACC 
GTCTGCTGCG ACGACGAAGC AGCTTCCGGC CCTGTCCGTC TCTTTACACC GTACAAGCGG 
CGGAAGCGGA ATCAAACTGA TCTCGATATG GAGTCTGGAC CCAAACGCAA GCGTAACACC 
CATCATAGTA ACAACAACAA CAGTAACACC AATAACAACA ACACGAGCGG GAGCGGCGCC 
AACAACTGTG TGGATGTAAC TGCAGCTGTG GCTGCTGCAA CAGCCAGCGT GGTGGACGAG 
AACAACATGT TCCTGTCGGA AGAGAACATC ACGTCCAAGG ATGAGCCGTG GGCAGCGCTC 
AACGACAGCC TGGACACCTC CACCGAGCTG GTCGATCAGT CGCAGATGGG CAACACGTAC 
GAACGGGAAA CGTTTGTGGT GAACATCAAC GATGGATCCT CCATCGCCGT CCTGGACACC 
AGCCAGTCGA TGAAGAACAT CGAGCACGTG TACTGCACCA TGGTCAAGGC CACCAATGAC 
TTCAAACGCA TGCTTAACGA CATGAAGCAG TCCTTCGAGC GTCGCATCGA GGTTCTGCAA 
AAGGAGCGCG ATGCAGCGGT CAGCGCGATG CGTGTCCAAG TGCACGCGGA CATCGATGAT 
CCAAATATTT CAGGCTCCCT GCACGGCAAC GAGATCATTT CGGCTAAGAA GTGCGCCAAC 
TGTAACCGCG AAGCATTGGC CGAGTGCTCA CTGTGCCGAA AGACGCCGTA CTGCTCGGAG 
TTCTGCCAGC GGAAGGATTG GAATGCTCAC CAGGTGGAGT GCACAAGGAA TCCACAAACG 
ACAACGCAGC AGGTCATGCT GTTGATCGAC GATCAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034033.1
CDS	362..2083
Translation	MEQVDSSTELHLNRKDLAALAEDVVKEEVILESSSHHHHHHHHQLDTKVRMVTSSSNDNSGSGGASGGTSGAGGGNGGGGVVSVPVSLPIGSMITGTTFNVITPDQLPPHFKPMLCVDNNGYLSGSTVSMGNDLKTIVIQQQQTQPGGGGGGANNAGTNTTATNTIGLNHDGSGSNNSHDSLATLEHAAGGASGVGGGGGGTGGGSSGWSENPSTQHNEVFQIRCKTTCAELYRSKLGSGGRGRCVKYKDKWHTPSEFEHVCGRGSSKDWKRSIKYGGKSLQSLIDEGTLTPHATNCSCTVCCDDEAASGPVRLFTPYKRRKRNQTDLDMESGPKRKRNTHHSNNNNSNTNNNNTSGSGANNCVDVTAAVAAATASVVDENNMFLSEENITSKDEPWAALNDSLDTSTELVDQSQMGNTYERETFVVNINDGSSIAVLDTSQSMKNIEHVYCTMVKATNDFKRMLNDMKQSFERRIEVLQKERDAAVSAMRVQVHADIDDPNISGSLHGNEIISAKKCANCNREALAECSLCRKTPYCSEFCQRKDWNAHQVECTRNPQTTTQQVMLLIDDQS

Target ORF information:

RefSeq Version	NM_001038944.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant B (Deaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038944.2

ORF Insert Sequence:

ATGGAACAGG TTGACTCCTC CACCGAGCTG CACCTGAATA GGAAAGATCT GGCCGCGCTG 
GCCGAGGATG TTGTGAAAGA AGAGGTGATA TTAGAAAGCT CCAGCCATCA CCATCATCAT 
CATCACCACC AGTTGGACAC CAAGGTTCGC ATGGTCACAT CCTCCTCCAA CGACAACAGT 
GGTAGCGGCG GCGCCAGTGG CGGTACAAGC GGAGCGGGCG GAGGAAACGG CGGTGGCGGT 
GTGGTTTCGG TGCCCGTATC CCTGCCCATC GGTTCGATGA TAACCGGCAC TACCTTCAAT 
GTGATCACGC CCGACCAGCT GCCCCCTCAC TTCAAGCCGA TGCTGTGCGT CGACAACAAT 
GGCTACCTGT CCGGCAGCAC GGTCAGCATG GGCAACGACC TCAAGACGAT CGTCATCCAG 
CAACAGCAGA CGCAACCAGG CGGCGGCGGT GGCGGCGCCA ATAATGCGGG CACCAATACG 
ACCGCCACAA ATACGATTGG ACTGAATCAC GATGGCTCCG GGAGCAACAA TAGCCACGAC 
AGCCTGGCCA CCTTGGAGCA CGCCGCGGGC GGAGCGAGTG GCGTCGGCGG TGGCGGCGGC 
GGAACGGGTG GTGGCTCCTC GGGATGGTCG GAGAACCCGT CAACCCAGCA CAATGAGGTC 
TTCCAGATCC GCTGCAAAAC CACCTGCGCC GAGTTATACC GCAGCAAGCT GGGCTCCGGC 
GGCCGTGGAC GCTGTGTGAA GTACAAGGAC AAGTGGCATA CGCCCAGTGA GTTTGAGCAT 
GTGTGTGGCC GGGGCTCCAG CAAGGATTGG AAGCGTTCGA TCAAGTACGG CGGCAAATCG 
CTGCAGTCCC TCATCGATGA GGGTACGCTC ACGCCGCACG CGACCAACTG CAGCTGCACC 
GTCTGCTGCG ACGACGAAGC AGCTTCCGGC CCTGTCCGTC TCTTTACACC GTACAAGCGG 
CGGAAGCGGA ATCAAACTGA TCTCGATATG GAGTCTGGAC CCAAACGCAA GCGTAACACC 
CATCATAGTA ACAACAACAA CAGTAACACC AATAACAACA ACACGAGCGG GAGCGGCGCC 
AACAACTGTG TGGATGTAAC TGCAGCTGTG GCTGCTGCAA CAGCCAGCGT GGTGGACGAG 
AACAACATGT TCCTGTCGGA AGAGAACATC ACGTCCAAGG ATGAGCCGTG GGCAGCGCTC 
AACGACAGCC TGGACACCTC CACCGAGCTG GTCGATCAGT CGCAGATGGG CAACACGTAC 
GAACGGGAAA CGTTTGTGGT GAACATCAAC GATGGATCCT CCATCGCCGT CCTGGACACC 
AGCCAGTCGA TGAAGAACAT CGAGCACGTG TACTGCACCA TGGTCAAGGC CACCAATGAC 
TTCAAACGCA TGCTTAACGA CATGAAGCAG TCCTTCGAGC GTCGCATCGA GGTTCTGCAA 
AAGGAGCGCG ATGCAGCGGT CAGCGCGATG CGTGTCCAAG TGCACGCGGA CATCGATGAT 
CCAAATATTT CAGGCTCCCT GCACGGCAAC GAGATCATTT CGGCTAAGAA GTGCGCCAAC 
TGTAACCGCG AAGCATTGGC CGAGTGCTCA CTGTGCCGAA AGACGCCGTA CTGCTCGGAG 
TTCTGCCAGC GGAAGGATTG GAATGCTCAC CAGGTGGAGT GCACAAGGAA TCCACAAACG 
ACAACGCAGC AGGTCATGCT GTTGATCGAC GATCAGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( NM_001275139.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262068.1 Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant C (Deaf1), mRNA.

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CloneID	OFa21100
Clone ID Related Accession (Same CDS sequence)	NM_001275139.1
Accession Version	NM_001275139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deformed epidermal autoregulatory factor-1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCTA TGGTAATGCA GTTGGACACC AAGGTTCGCA TGGTCACATC CTCCTCCAAC 
GACAACAGTG GTAGCGGCGG CGCCAGTGGC GGTACAAGCG GAGCGGGCGG AGGAAACGGC 
GGTGGCGGTG TGGTTTCGGT GCCCGTATCC CTGCCCATCG GTTCGATGAT AACCGGCACT 
ACCTTCAATG TGATCACGCC CGACCAGCTG CCCCCTCACT TCAAGCCGAT GCTGTGCGTC 
GACAACAATG GCTACCTGTC CGGCAGCACG GTCAGCATGG GCAACGACCT CAAGACGATC 
GTCATCCAGC AACAGCAGAC GCAACCAGGC GGCGGCGGTG GCGGCGCCAA TAATGCGGGC 
ACCAATACGA CCGCCACAAA TACGATTGGA CTGAATCACG ATGGCTCCGG GAGCAACAAT 
AGCCACGACA GCCTGGCCAC CTTGGAGCAC GCCGCGGGCG GAGCGAGTGG CGTCGGCGGT 
GGCGGCGGCG GAACGGGTGG TGGCTCCTCG GGATGGTCGG AGAACCCGTC AACCCAGCAC 
AATGAGGTCT TCCAGATCCG CTGCAAAACC ACCTGCGCCG AGTTATACCG CAGCAAGCTG 
GGCTCCGGCG GCCGTGGACG CTGTGTGAAG TACAAGGACA AGTGGCATAC GCCCAGTGAG 
TTTGAGCATG TGTGTGGCCG GGGCTCCAGC AAGGATTGGA AGCGTTCGAT CAAGTACGGC 
GGCAAATCGC TGCAGTCCCT CATCGATGAG GGTACGCTCA CGCCGCACGC GACCAACTGC 
AGCTGCACCG TCTGCTGCGA CGACGAAGCA GGTGAGTCAG CTTCCGGCCC TGTCCGTCTC 
TTTACACCGT ACAAGCGGCG GAAGCGGAAT CAAACTGATC TCGATATGGA GTCTGGACCC 
AAACGCAAGC GTAACACCCA TCATAGTAAC AACAACAACA GTAACACCAA TAACAACAAC 
ACGAGCGGGA GCGGCGCCAA CAACTGTGTG GATGTAACTG CAGCTGTGGC TGCTGCAACA 
GCCAGCGTGG TGGACGAGAA CAACATGTTC CTGTCGGAAG AGAACATCAC GTCCAAGGAT 
GAGCCGTGGG CAGCGCTCAA CGACAGCCTG GACACCTCCA CCGAGCTGGT CGATCAGTCG 
CAGATGGGCA ACACGTACGA ACGGGAAACG TTTGTGGTGA ACATCAACGA TGGATCCTCC 
ATCGCCGTCC TGGACACCAG CCAGTCGATG AAGAACATCG AGCACGTGTA CTGCACCATG 
GTCAAGGCCA CCAATGACTT CAAACGCATG CTTAACGACA TGAAGCAGTC CTTCGAGCGT 
CGCATCGAGG TTCTGCAAAA GGAGCGCGAT GCAGCGGTCA GCGCGATGCG TGTCCAAGTG 
CACGCGGACA TCGATGATCC AAATATTTCA GGCTCCCTGC ACGGCAACGA GATCATTTCG 
GCTAAGAAGT GCGCCAACTG TAACCGCGAA GCATTGGCCG AGTGCTCACT GTGCCGAAAG 
ACGCCGTACT GCTCGGAGTT CTGCCAGCGG AAGGATTGGA ATGCTCACCA GGTGGAGTGC 
ACAAGGAATC CACAAACGAC AACGCAGCAG GTCATGCTGT TGATCGACGA TCAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262068.1
CDS	90..1709
Translation	MCAMVMQLDTKVRMVTSSSNDNSGSGGASGGTSGAGGGNGGGGVVSVPVSLPIGSMITGTTFNVITPDQLPPHFKPMLCVDNNGYLSGSTVSMGNDLKTIVIQQQQTQPGGGGGGANNAGTNTTATNTIGLNHDGSGSNNSHDSLATLEHAAGGASGVGGGGGGTGGGSSGWSENPSTQHNEVFQIRCKTTCAELYRSKLGSGGRGRCVKYKDKWHTPSEFEHVCGRGSSKDWKRSIKYGGKSLQSLIDEGTLTPHATNCSCTVCCDDEAGESASGPVRLFTPYKRRKRNQTDLDMESGPKRKRNTHHSNNNNSNTNNNNTSGSGANNCVDVTAAVAAATASVVDENNMFLSEENITSKDEPWAALNDSLDTSTELVDQSQMGNTYERETFVVNINDGSSIAVLDTSQSMKNIEHVYCTMVKATNDFKRMLNDMKQSFERRIEVLQKERDAAVSAMRVQVHADIDDPNISGSLHGNEIISAKKCANCNREALAECSLCRKTPYCSEFCQRKDWNAHQVECTRNPQTTTQQVMLLIDDQS

Target ORF information:

RefSeq Version	NM_001275139.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant C (Deaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275139.1

ORF Insert Sequence:

ATGTGCGCTA TGGTAATGCA GTTGGACACC AAGGTTCGCA TGGTCACATC CTCCTCCAAC 
GACAACAGTG GTAGCGGCGG CGCCAGTGGC GGTACAAGCG GAGCGGGCGG AGGAAACGGC 
GGTGGCGGTG TGGTTTCGGT GCCCGTATCC CTGCCCATCG GTTCGATGAT AACCGGCACT 
ACCTTCAATG TGATCACGCC CGACCAGCTG CCCCCTCACT TCAAGCCGAT GCTGTGCGTC 
GACAACAATG GCTACCTGTC CGGCAGCACG GTCAGCATGG GCAACGACCT CAAGACGATC 
GTCATCCAGC AACAGCAGAC GCAACCAGGC GGCGGCGGTG GCGGCGCCAA TAATGCGGGC 
ACCAATACGA CCGCCACAAA TACGATTGGA CTGAATCACG ATGGCTCCGG GAGCAACAAT 
AGCCACGACA GCCTGGCCAC CTTGGAGCAC GCCGCGGGCG GAGCGAGTGG CGTCGGCGGT 
GGCGGCGGCG GAACGGGTGG TGGCTCCTCG GGATGGTCGG AGAACCCGTC AACCCAGCAC 
AATGAGGTCT TCCAGATCCG CTGCAAAACC ACCTGCGCCG AGTTATACCG CAGCAAGCTG 
GGCTCCGGCG GCCGTGGACG CTGTGTGAAG TACAAGGACA AGTGGCATAC GCCCAGTGAG 
TTTGAGCATG TGTGTGGCCG GGGCTCCAGC AAGGATTGGA AGCGTTCGAT CAAGTACGGC 
GGCAAATCGC TGCAGTCCCT CATCGATGAG GGTACGCTCA CGCCGCACGC GACCAACTGC 
AGCTGCACCG TCTGCTGCGA CGACGAAGCA GGTGAGTCAG CTTCCGGCCC TGTCCGTCTC 
TTTACACCGT ACAAGCGGCG GAAGCGGAAT CAAACTGATC TCGATATGGA GTCTGGACCC 
AAACGCAAGC GTAACACCCA TCATAGTAAC AACAACAACA GTAACACCAA TAACAACAAC 
ACGAGCGGGA GCGGCGCCAA CAACTGTGTG GATGTAACTG CAGCTGTGGC TGCTGCAACA 
GCCAGCGTGG TGGACGAGAA CAACATGTTC CTGTCGGAAG AGAACATCAC GTCCAAGGAT 
GAGCCGTGGG CAGCGCTCAA CGACAGCCTG GACACCTCCA CCGAGCTGGT CGATCAGTCG 
CAGATGGGCA ACACGTACGA ACGGGAAACG TTTGTGGTGA ACATCAACGA TGGATCCTCC 
ATCGCCGTCC TGGACACCAG CCAGTCGATG AAGAACATCG AGCACGTGTA CTGCACCATG 
GTCAAGGCCA CCAATGACTT CAAACGCATG CTTAACGACA TGAAGCAGTC CTTCGAGCGT 
CGCATCGAGG TTCTGCAAAA GGAGCGCGAT GCAGCGGTCA GCGCGATGCG TGTCCAAGTG 
CACGCGGACA TCGATGATCC AAATATTTCA GGCTCCCTGC ACGGCAACGA GATCATTTCG 
GCTAAGAAGT GCGCCAACTG TAACCGCGAA GCATTGGCCG AGTGCTCACT GTGCCGAAAG 
ACGCCGTACT GCTCGGAGTT CTGCCAGCGG AAGGATTGGA ATGCTCACCA GGTGGAGTGC 
ACAAGGAATC CACAAACGAC AACGCAGCAG GTCATGCTGT TGATCGACGA TCAGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Deaf1 ( NM_001275141.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262070.1 Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant E (Deaf1), mRNA.

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CloneID	OFa21101
Clone ID Related Accession (Same CDS sequence)	NM_001275141.1
Accession Version	NM_001275141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deformed epidermal autoregulatory factor-1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCTA TGGTAATGCA GTTGGACACC AAGGTTCGCA TGGTCACATC CTCCTCCAAC 
GACAACAGTG GTAGCGGCGG CGCCAGTGGC GGTACAAGCG GAGCGGGCGG AGGAAACGGC 
GGTGGCGGTG TGGTTTCGGT GCCCGTATCC CTGCCCATCG GTTCGATGAT AACCGGCACT 
ACCTTCAATG TGATCACGCC CGACCAGCTG CCCCCTCACT TCAAGCCGAT GCTGTGCGTC 
GACAACAATG GCTACCTGTC CGGCAGCACG GTCAGCATGG GCAACGACCT CAAGACGATC 
GTCATCCAGC AACAGCAGAC GCAACCAGGC GGCGGCGGTG GCGGCGCCAA TAATGCGGGC 
ACCAATACGA CCGCCACAAA TACGATTGGA CTGAATCACG ATGGCTCCGG GAGCAACAAT 
AGCCACGACA GCCTGGCCAC CTTGGAGCAC GCCGCGGGCG GAGCGAGTGG CGTCGGCGGT 
GGCGGCGGCG GAACGGGTGG TGGCTCCTCG GGATGGTCGG AGAACCCGTC AACCCAGCAC 
AATGAGGTCT TCCAGATCCG CTGCAAAACC ACCTGCGCCG AGTTATACCG CAGCAAGCTG 
GGCTCCGGCG GCCGTGGACG CTGTGTGAAG TACAAGGACA AGTGGCATAC GCCCAGTGAG 
TTTGAGCATG TGTGTGGCCG GGGCTCCAGC AAGGATTGGA AGCGTTCGAT CAAGTACGGC 
GGCAAATCGC TGCAGTCCCT CATCGATGAG GGTACGCTCA CGCCGCACGC GACCAACTGC 
AGCTGCACCG TCTGCTGCGA CGACGAAGCA GCTTCCGGCC CTGTCCGTCT CTTTACACCG 
TACAAGCGGC GGAAGCGGAA TCAAACTGAT CTCGATATGG AGTCTGGACC CAAACGCAAG 
CGTAACACCC ATCATAGTAA CAACAACAAC AGTAACACCA ATAACAACAA CACGAGCGGG 
AGCGGCGCCA ACAACTGTGT GGATGTAACT GCAGCTGTGG CTGCTGCAAC AGCCAGCGTG 
GTGGACGAGA ACAACATGTT CCTGTCGGAA GAGAACATCA CGTCCAAGGA TGAGCCGTGG 
GCAGCGCTCA ACGACAGCCT GGACACCTCC ACCGAGCTGG TCGATCAGTC GCAGATGGGC 
AACACGTACG AACGGGAAAC GTTTGTGGTG AACATCAACG ATGGATCCTC CATCGCCGTC 
CTGGACACCA GCCAGTCGAT GAAGAACATC GAGCACGTGT ACTGCACCAT GGTCAAGGCC 
ACCAATGACT TCAAACGCAT GCTTAACGAC ATGAAGCAGT CCTTCGAGCG TCGCATCGAG 
GTTCTGCAAA AGGAGCGCGA TGCAGCGGTC AGCGCGATGC GTGTCCAAGT GCACGCGGAC 
ATCGATGATC CAAATATTTC AGGCTCCCTG CACGGCAACG AGATCATTTC GGCTAAGAAG 
TGCGCCAACT GTAACCGCGA AGCATTGGCC GAGTGCTCAC TGTGCCGAAA GACGCCGTAC 
TGCTCGGAGT TCTGCCAGCG GAAGGATTGG AATGCTCACC AGGTGGAGTG CACAAGGAAT 
CCACAAACGA CAACGCAGCA GGTCATGCTG TTGATCGACG ATCAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262070.1
CDS	90..1700
Translation	MCAMVMQLDTKVRMVTSSSNDNSGSGGASGGTSGAGGGNGGGGVVSVPVSLPIGSMITGTTFNVITPDQLPPHFKPMLCVDNNGYLSGSTVSMGNDLKTIVIQQQQTQPGGGGGGANNAGTNTTATNTIGLNHDGSGSNNSHDSLATLEHAAGGASGVGGGGGGTGGGSSGWSENPSTQHNEVFQIRCKTTCAELYRSKLGSGGRGRCVKYKDKWHTPSEFEHVCGRGSSKDWKRSIKYGGKSLQSLIDEGTLTPHATNCSCTVCCDDEAASGPVRLFTPYKRRKRNQTDLDMESGPKRKRNTHHSNNNNSNTNNNNTSGSGANNCVDVTAAVAAATASVVDENNMFLSEENITSKDEPWAALNDSLDTSTELVDQSQMGNTYERETFVVNINDGSSIAVLDTSQSMKNIEHVYCTMVKATNDFKRMLNDMKQSFERRIEVLQKERDAAVSAMRVQVHADIDDPNISGSLHGNEIISAKKCANCNREALAECSLCRKTPYCSEFCQRKDWNAHQVECTRNPQTTTQQVMLLIDDQS

Target ORF information:

RefSeq Version	NM_001275141.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deformed epidermal autoregulatory factor-1, transcript variant E (Deaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275141.1

ORF Insert Sequence:

ATGTGCGCTA TGGTAATGCA GTTGGACACC AAGGTTCGCA TGGTCACATC CTCCTCCAAC 
GACAACAGTG GTAGCGGCGG CGCCAGTGGC GGTACAAGCG GAGCGGGCGG AGGAAACGGC 
GGTGGCGGTG TGGTTTCGGT GCCCGTATCC CTGCCCATCG GTTCGATGAT AACCGGCACT 
ACCTTCAATG TGATCACGCC CGACCAGCTG CCCCCTCACT TCAAGCCGAT GCTGTGCGTC 
GACAACAATG GCTACCTGTC CGGCAGCACG GTCAGCATGG GCAACGACCT CAAGACGATC 
GTCATCCAGC AACAGCAGAC GCAACCAGGC GGCGGCGGTG GCGGCGCCAA TAATGCGGGC 
ACCAATACGA CCGCCACAAA TACGATTGGA CTGAATCACG ATGGCTCCGG GAGCAACAAT 
AGCCACGACA GCCTGGCCAC CTTGGAGCAC GCCGCGGGCG GAGCGAGTGG CGTCGGCGGT 
GGCGGCGGCG GAACGGGTGG TGGCTCCTCG GGATGGTCGG AGAACCCGTC AACCCAGCAC 
AATGAGGTCT TCCAGATCCG CTGCAAAACC ACCTGCGCCG AGTTATACCG CAGCAAGCTG 
GGCTCCGGCG GCCGTGGACG CTGTGTGAAG TACAAGGACA AGTGGCATAC GCCCAGTGAG 
TTTGAGCATG TGTGTGGCCG GGGCTCCAGC AAGGATTGGA AGCGTTCGAT CAAGTACGGC 
GGCAAATCGC TGCAGTCCCT CATCGATGAG GGTACGCTCA CGCCGCACGC GACCAACTGC 
AGCTGCACCG TCTGCTGCGA CGACGAAGCA GCTTCCGGCC CTGTCCGTCT CTTTACACCG 
TACAAGCGGC GGAAGCGGAA TCAAACTGAT CTCGATATGG AGTCTGGACC CAAACGCAAG 
CGTAACACCC ATCATAGTAA CAACAACAAC AGTAACACCA ATAACAACAA CACGAGCGGG 
AGCGGCGCCA ACAACTGTGT GGATGTAACT GCAGCTGTGG CTGCTGCAAC AGCCAGCGTG 
GTGGACGAGA ACAACATGTT CCTGTCGGAA GAGAACATCA CGTCCAAGGA TGAGCCGTGG 
GCAGCGCTCA ACGACAGCCT GGACACCTCC ACCGAGCTGG TCGATCAGTC GCAGATGGGC 
AACACGTACG AACGGGAAAC GTTTGTGGTG AACATCAACG ATGGATCCTC CATCGCCGTC 
CTGGACACCA GCCAGTCGAT GAAGAACATC GAGCACGTGT ACTGCACCAT GGTCAAGGCC 
ACCAATGACT TCAAACGCAT GCTTAACGAC ATGAAGCAGT CCTTCGAGCG TCGCATCGAG 
GTTCTGCAAA AGGAGCGCGA TGCAGCGGTC AGCGCGATGC GTGTCCAAGT GCACGCGGAC 
ATCGATGATC CAAATATTTC AGGCTCCCTG CACGGCAACG AGATCATTTC GGCTAAGAAG 
TGCGCCAACT GTAACCGCGA AGCATTGGCC GAGTGCTCAC TGTGCCGAAA GACGCCGTAC 
TGCTCGGAGT TCTGCCAGCG GAAGGATTGG AATGCTCACC AGGTGGAGTG CACAAGGAAT 
CCACAAACGA CAACGCAGCA GGTCATGCTG TTGATCGACG ATCAGTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Deaf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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