Hsp27 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp27
Entrez Gene ID	39078
Full Name	Heat shock protein 27
Synonyms	27,27K,28,CG4466,Dhsp27,DmHSP27,DmHsp27,Dmel23.6,Dmel\CG4466,HSP27,HSP28,Hsp 27,Hsp28,anon-WO0140519.69,hsp 27,hsp-27,hsp26,hsp27,hsp27/26,hsp28,small hsp locus 67B
General protein information	Preferred Names Heat shock protein 27 Names CG4466 gene product from transcript CG4466-RB CG4466-PA CG4466-PB Hsp 27 Hsp27-PA Hsp27-PB heat shock protein 237 heat shock protein 27 heat shock protein hsp27 heatshock protein 27 protein 27
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B3-67B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_079276.4	NP_524000.1	heat shock protein 27, isoform A
NM_001300072.1	NP_001287001.1	heat shock protein 27, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hsp23	NP_001246694.1
Drosophila melanogaster (fruit fly)	Hsp27	NP_524000.1
Drosophila melanogaster (fruit fly)	Hsp22	NP_001027114.1
Drosophila melanogaster (fruit fly)	Hsp26	NP_523997.1
Drosophila melanogaster (fruit fly)	Hsp67Ba	NP_523998.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that while Hsp70 or Hsp83 expression under normal or stress conditions was not affected by AR feeding, Hsp27 levels were elevated in AR-fed wild-type control as well as heat-shocked larvae
Title: Ayurvedic

The study evidenced the neuroprotective efficacy of hsp27 overexpression against prolonged dichlorvos exposure.
Title: Overexpression of hsp27 Rescued Neuronal Cell Death and Reduction in Life- and Health-Span in Drosophila melanogaster Against Prolonged Exposure to Dichlorvos.

placental HSP 27 may play a role in labor and is spatially controlled
Title: Heat shock protein 27 is spatially distributed in the human placenta and decreased during labor.

We propose that Atg7 acts downstream of Hsp27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila.
Title: Autophagy-related gene 7 is downstream of heat shock protein 27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila.

The functional similarities and differences of neuronal expression of hsp26 and hsp27 in adult Drosophila were demonstrated.
Title: The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila.

The following Hsp27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18024	NM_001300072.1 Latest version!	Drosophila melanogaster heat shock protein 27, transcript variant B (Hsp27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa18024	NM_079276.4 Latest version!	Drosophila melanogaster heat shock protein 27, transcript variant A (Hsp27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp27 ( NM_079276.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524000.1 Drosophila melanogaster heat shock protein 27, transcript variant A (Hsp27), mRNA.

Price & Availability↑

CloneID	OFa18024
Clone ID Related Accession (Same CDS sequence)	NM_079276.4 , NM_001300072.1
Accession Version	NM_079276.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 27, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079276.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATTA TACCACTGCT GCACTTGGCC CGGGAGTTGG ATCATGACTA CCGCACCGAC 
TGGGGGCATT TGCTGGAGGA TGACTTCGGT TTTGGCGTCC ATGCCCACGA TCTGTTCCAT 
CCGCGTCGCC TGCTACTGCC CAACACCCTG GGACTGGGTC GTCGTCGTTA TTCGCCGTAC 
GAGAGGAGCC ATGGCCACCA CAATCAAATG TCACGTCGCG CGTCGGGGGG TCCAAACGCT 
CTGCTGCCCG CCGTGGGCAA AGATGGCTTC CAGGTGTGCA TGGATGTGTC GCAGTTCAAG 
CCCAACGAGC TGACCGTCAA GGTGGTGGAC AACACCGTGG TGGTAGAGGG GAAGCACGAG 
GAGCGCGAGG ACGGCCATGG AATGATCCAG CGTCACTTTG TGCGCAAGTA TACCCTGCCC 
AAGGGCTTTG ACCCCAACGA GGTAGTGTCC ACTGTCTCAT CCGACGGTGT GCTGACCCTC 
AAGGCCCCGC CGCCGCCCAG CAAGGAACAG GCCAAGTCGG AGCGCATTGT CCAGATCCAG 
CAAACGGGGC CTGCCCATTT GAGCGTCAAG GCACCGGCAC CCGAGGCTGG CGATGGAAAA 
GCCGAAAATG GCAGCGGCGA GAAAATGGAG ACTAGCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524000.1
CDS	124..765
Translation	MSIIPLLHLARELDHDYRTDWGHLLEDDFGFGVHAHDLFHPRRLLLPNTLGLGRRRYSPYERSHGHHNQMSRRASGGPNALLPAVGKDGFQVCMDVSQFKPNELTVKVVDNTVVVEGKHEEREDGHGMIQRHFVRKYTLPKGFDPNEVVSTVSSDGVLTLKAPPPPSKEQAKSERIVQIQQTGPAHLSVKAPAPEAGDGKAENGSGEKMETSK

Target ORF information:

RefSeq Version	NM_079276.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 27, transcript variant A (Hsp27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079276.4

ORF Insert Sequence:

ATGTCAATTA TACCACTGCT GCACTTGGCC CGGGAGTTGG ATCATGACTA CCGCACCGAC 
TGGGGGCATT TGCTGGAGGA TGACTTCGGT TTTGGCGTCC ATGCCCACGA TCTGTTCCAT 
CCGCGTCGCC TGCTACTGCC CAACACCCTG GGACTGGGTC GTCGTCGTTA TTCGCCGTAC 
GAGAGGAGCC ATGGCCACCA CAATCAAATG TCACGTCGCG CGTCGGGGGG TCCAAACGCT 
CTGCTGCCCG CCGTGGGCAA AGATGGCTTC CAGGTGTGCA TGGATGTGTC GCAGTTCAAG 
CCCAACGAGC TGACCGTCAA GGTGGTGGAC AACACCGTGG TGGTAGAGGG GAAGCACGAG 
GAGCGCGAGG ACGGCCATGG AATGATCCAG CGTCACTTTG TGCGCAAGTA TACCCTGCCC 
AAGGGCTTTG ACCCCAACGA GGTAGTGTCC ACTGTCTCAT CCGACGGTGT GCTGACCCTC 
AAGGCCCCGC CGCCGCCCAG CAAGGAACAG GCCAAGTCGG AGCGCATTGT CCAGATCCAG 
CAAACGGGGC CTGCCCATTT GAGCGTCAAG GCACCGGCAC CCGAGGCTGG CGATGGAAAA 
GCCGAAAATG GCAGCGGCGA GAAAATGGAG ACTAGCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsp27 ( NM_001300072.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287001.1 Drosophila melanogaster heat shock protein 27, transcript variant B (Hsp27), mRNA.

Price & Availability↑

CloneID	OFa18024
Clone ID Related Accession (Same CDS sequence)	NM_079276.4 , NM_001300072.1
Accession Version	NM_001300072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 27, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAATTA TACCACTGCT GCACTTGGCC CGGGAGTTGG ATCATGACTA CCGCACCGAC 
TGGGGGCATT TGCTGGAGGA TGACTTCGGT TTTGGCGTCC ATGCCCACGA TCTGTTCCAT 
CCGCGTCGCC TGCTACTGCC CAACACCCTG GGACTGGGTC GTCGTCGTTA TTCGCCGTAC 
GAGAGGAGCC ATGGCCACCA CAATCAAATG TCACGTCGCG CGTCGGGGGG TCCAAACGCT 
CTGCTGCCCG CCGTGGGCAA AGATGGCTTC CAGGTGTGCA TGGATGTGTC GCAGTTCAAG 
CCCAACGAGC TGACCGTCAA GGTGGTGGAC AACACCGTGG TGGTAGAGGG GAAGCACGAG 
GAGCGCGAGG ACGGCCATGG AATGATCCAG CGTCACTTTG TGCGCAAGTA TACCCTGCCC 
AAGGGCTTTG ACCCCAACGA GGTAGTGTCC ACTGTCTCAT CCGACGGTGT GCTGACCCTC 
AAGGCCCCGC CGCCGCCCAG CAAGGAACAG GCCAAGTCGG AGCGCATTGT CCAGATCCAG 
CAAACGGGGC CTGCCCATTT GAGCGTCAAG GCACCGGCAC CCGAGGCTGG CGATGGAAAA 
GCCGAAAATG GCAGCGGCGA GAAAATGGAG ACTAGCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287001.1
CDS	124..765
Translation	MSIIPLLHLARELDHDYRTDWGHLLEDDFGFGVHAHDLFHPRRLLLPNTLGLGRRRYSPYERSHGHHNQMSRRASGGPNALLPAVGKDGFQVCMDVSQFKPNELTVKVVDNTVVVEGKHEEREDGHGMIQRHFVRKYTLPKGFDPNEVVSTVSSDGVLTLKAPPPPSKEQAKSERIVQIQQTGPAHLSVKAPAPEAGDGKAENGSGEKMETSK

Target ORF information:

RefSeq Version	NM_001300072.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 27, transcript variant B (Hsp27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300072.1

ORF Insert Sequence:

ATGTCAATTA TACCACTGCT GCACTTGGCC CGGGAGTTGG ATCATGACTA CCGCACCGAC 
TGGGGGCATT TGCTGGAGGA TGACTTCGGT TTTGGCGTCC ATGCCCACGA TCTGTTCCAT 
CCGCGTCGCC TGCTACTGCC CAACACCCTG GGACTGGGTC GTCGTCGTTA TTCGCCGTAC 
GAGAGGAGCC ATGGCCACCA CAATCAAATG TCACGTCGCG CGTCGGGGGG TCCAAACGCT 
CTGCTGCCCG CCGTGGGCAA AGATGGCTTC CAGGTGTGCA TGGATGTGTC GCAGTTCAAG 
CCCAACGAGC TGACCGTCAA GGTGGTGGAC AACACCGTGG TGGTAGAGGG GAAGCACGAG 
GAGCGCGAGG ACGGCCATGG AATGATCCAG CGTCACTTTG TGCGCAAGTA TACCCTGCCC 
AAGGGCTTTG ACCCCAACGA GGTAGTGTCC ACTGTCTCAT CCGACGGTGT GCTGACCCTC 
AAGGCCCCGC CGCCGCCCAG CAAGGAACAG GCCAAGTCGG AGCGCATTGT CCAGATCCAG 
CAAACGGGGC CTGCCCATTT GAGCGTCAAG GCACCGGCAC CCGAGGCTGG CGATGGAAAA 
GCCGAAAATG GCAGCGGCGA GAAAATGGAG ACTAGCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsp27 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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