ena cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ena
Entrez Gene ID	37201
Full Name	enabled
Synonyms	CG15112,Dmel\CG15112,ENA/VASP,Ena,Enb,VASP,Vasp,enb,l(2)02029
General protein information	Preferred Names enabled Names CG15112 gene product from transcript CG15112-RG CG15112-PA CG15112-PB CG15112-PC CG15112-PD CG15112-PE CG15112-PF CG15112-PG VASP ena-PA ena-PB ena-PC ena-PD ena-PE ena-PF ena-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56B5-56C1\|2-87 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_166331.3	NP_725859.2	enabled, isoform B
NM_001014537.3	NP_001014537.1	enabled, isoform D
NM_001014536.2	NP_001014536.1	enabled, isoform E
NM_166329.2	NP_725857.1	enabled, isoform A
NM_166330.2	NP_725858.1	enabled, isoform C
NM_001144237.2	NP_001137709.1	enabled, isoform F
NM_001299673.1	NP_001286602.1	enabled, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Abl tyrosine kinase signaling network controls cell migration, epithelial organization, axon patterning and other aspects of development. Abl suppresses the actin-regulatory factor Enabled, and we find that Abl also acts through the GEF Trio to stimulate the signaling activity of Rac GTPase.
Title: The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila.

Abl/Ena signaling controls the subcellular architecture of the Golgi complex in Drosophila photoreceptor neurons through its regulation of the Golgi-associated actin cytoskeleton.
Title: The Abl/enabled signaling pathway regulates Golgi architecture in Drosophila photoreceptor neurons.

the activities of Ena/VASP and the WAVE regulatory complex are intimately linked to ensure optimal control of actin polymerization during cell migration and development
Title: Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.

Ena is both sufficient and necessary to account for miR-8-mediated regulation of subsynaptic reticulum architecture.
Title: miR-8 controls synapse structure by repression of the actin regulator enabled.

Fascin and Ena cooperate to extend and maintain robust filopodia of uniform thickness with aligned barbed ends by a unique mechanistic cycle.
Title: Ena/VASP Enabled is a highly processive actin polymerase tailored to self-assemble parallel-bundled F-actin networks with Fascin.

The following ena gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ena cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10580	NM_166330.2 Latest version!	Drosophila melanogaster enabled (ena), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa10580	NM_001014536.2 Latest version!	Drosophila melanogaster enabled (ena), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa10580	NM_166329.2 Latest version!	Drosophila melanogaster enabled (ena), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa10581	NM_001014537.3 Latest version!	Drosophila melanogaster enabled (ena), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa10582	NM_001144237.2 Latest version!	Drosophila melanogaster enabled (ena), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa10583	NM_166331.3 Latest version!	Drosophila melanogaster enabled (ena), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa21863	NM_001299673.1 Latest version!	Drosophila melanogaster enabled (ena), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ena ( NM_001014536.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014536.1 Drosophila melanogaster enabled (ena), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa10580
Clone ID Related Accession (Same CDS sequence)	NM_001014536.2 , NM_166329.2 , NM_166330.2
Accession Version	NM_001014536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001014536.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG CGCCCCACCG GCACCACAGC CCCAACAGCA GCAGCAGCAG 
CAGCAGGCGC AGCAAATGGG TCAGCCAGGA TCCCACTATG GACCCACTGG AAATGGACCC 
ACTTCGAATG GCCTGCCACA GCAGGTGAAC TCACAGATTC CGCCGGCACC GCAGCAGCAG 
CCACAACAGC AACAGTTTCA GCAGCAGCAG CAGCAACAGC AATATCAGCA AATGGTCCAG 
GCGGGCTATG CGCCCTCTCA GCAGTATCAG CAACCCCACT ACGTGCTCTC CAATTCTAAT 
CCCAATCTAA CTGTGCACCA ATACCCGACA CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014536.1
CDS	482..2536
Translation	MTEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_001014536.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014536.2

ORF Insert Sequence:

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG CGCCCCACCG GCACCACAGC CCCAACAGCA GCAGCAGCAG 
CAGCAGGCGC AGCAAATGGG TCAGCCAGGA TCCCACTATG GACCCACTGG AAATGGACCC 
ACTTCGAATG GCCTGCCACA GCAGGTGAAC TCACAGATTC CGCCGGCACC GCAGCAGCAG 
CCACAACAGC AACAGTTTCA GCAGCAGCAG CAGCAACAGC AATATCAGCA AATGGTCCAG 
GCGGGCTATG CGCCCTCTCA GCAGTATCAG CAACCCCACT ACGTGCTCTC CAATTCTAAT 
CCCAATCTAA CTGTGCACCA ATACCCGACA CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ena ( NM_166329.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725857.1 Drosophila melanogaster enabled (ena), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10580
Clone ID Related Accession (Same CDS sequence)	NM_001014536.2 , NM_166329.2 , NM_166330.2
Accession Version	NM_166329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166329.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG CGCCCCACCG GCACCACAGC CCCAACAGCA GCAGCAGCAG 
CAGCAGGCGC AGCAAATGGG TCAGCCAGGA TCCCACTATG GACCCACTGG AAATGGACCC 
ACTTCGAATG GCCTGCCACA GCAGGTGAAC TCACAGATTC CGCCGGCACC GCAGCAGCAG 
CCACAACAGC AACAGTTTCA GCAGCAGCAG CAGCAACAGC AATATCAGCA AATGGTCCAG 
GCGGGCTATG CGCCCTCTCA GCAGTATCAG CAACCCCACT ACGTGCTCTC CAATTCTAAT 
CCCAATCTAA CTGTGCACCA ATACCCGACA CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725857.1
CDS	406..2460
Translation	MTEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_166329.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166329.2

ORF Insert Sequence:

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG CGCCCCACCG GCACCACAGC CCCAACAGCA GCAGCAGCAG 
CAGCAGGCGC AGCAAATGGG TCAGCCAGGA TCCCACTATG GACCCACTGG AAATGGACCC 
ACTTCGAATG GCCTGCCACA GCAGGTGAAC TCACAGATTC CGCCGGCACC GCAGCAGCAG 
CCACAACAGC AACAGTTTCA GCAGCAGCAG CAGCAACAGC AATATCAGCA AATGGTCCAG 
GCGGGCTATG CGCCCTCTCA GCAGTATCAG CAACCCCACT ACGTGCTCTC CAATTCTAAT 
CCCAATCTAA CTGTGCACCA ATACCCGACA CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ena ( NM_166330.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725858.1 Drosophila melanogaster enabled (ena), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10580
Clone ID Related Accession (Same CDS sequence)	NM_001014536.2 , NM_166329.2 , NM_166330.2
Accession Version	NM_166330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166330.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG CGCCCCACCG GCACCACAGC CCCAACAGCA GCAGCAGCAG 
CAGCAGGCGC AGCAAATGGG TCAGCCAGGA TCCCACTATG GACCCACTGG AAATGGACCC 
ACTTCGAATG GCCTGCCACA GCAGGTGAAC TCACAGATTC CGCCGGCACC GCAGCAGCAG 
CCACAACAGC AACAGTTTCA GCAGCAGCAG CAGCAACAGC AATATCAGCA AATGGTCCAG 
GCGGGCTATG CGCCCTCTCA GCAGTATCAG CAACCCCACT ACGTGCTCTC CAATTCTAAT 
CCCAATCTAA CTGTGCACCA ATACCCGACA CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725858.1
CDS	842..2896
Translation	MTEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_166330.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166330.2

ORF Insert Sequence:

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG CGCCCCACCG GCACCACAGC CCCAACAGCA GCAGCAGCAG 
CAGCAGGCGC AGCAAATGGG TCAGCCAGGA TCCCACTATG GACCCACTGG AAATGGACCC 
ACTTCGAATG GCCTGCCACA GCAGGTGAAC TCACAGATTC CGCCGGCACC GCAGCAGCAG 
CCACAACAGC AACAGTTTCA GCAGCAGCAG CAGCAACAGC AATATCAGCA AATGGTCCAG 
GCGGGCTATG CGCCCTCTCA GCAGTATCAG CAACCCCACT ACGTGCTCTC CAATTCTAAT 
CCCAATCTAA CTGTGCACCA ATACCCGACA CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ena ( NM_001014537.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014537.1 Drosophila melanogaster enabled (ena), transcript variant D, mRNA.

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CloneID	OFa10581
Clone ID Related Accession (Same CDS sequence)	NM_001014537.3
Accession Version	NM_001014537.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001014537.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG GAATTCCCTC AGCGCCCCAC CGGCACCACA GCCCCAACAG 
CAGCAGCAGC AGCAGCAGGC GCAGCAAATG GGTCAGCCAG GATCCCACTA TGGACCCACT 
GGAAATGGAC CCACTTCGAA TGGCCTGCCA CAGCAGGTGA ACTCACAGAT TCCGCCGGCA 
CCGCAGCAGC AGCCACAACA GCAACAGTTT CAGCAGCAGC AGCAGCAACA GCAATATCAG 
CAAATGGTCC AGGCGGGCTA TGCGCCCTCT CAGTATCAGC AACCCCACTA CGTGCTCTCC 
AATTCTAATC CCAATCTAAC TGTGCACCAA TACCCGACAC AGCAGGCGCA GCAGCAGCCA 
CCGCAAGCGC CACAGCCGCC GCTCCAGAAC GGTGGAATGT ACATGGTGGG TCATGGTCAT 
CTACCGAGCT CCGCGAGCGC CAACAGTGTT GTGTACGCCA GCCAGCAGCA GATGCTCCCG 
CAGGCGCATC CACAGGCACC TCAGGCGCCG ACGATGCCAG GTCCGGGCTA CGGTGGTCCA 
CCAGTACCTC CGCCCCAGCA GCAGGCAGAG AATCCGTACG GTCAGGTACC CATGCCGCCG 
CCAATGAATC CGTCACAGCA ACAGCAGCCA GGTCAGGTGC CTCTAAACCG GATGAGCAGC 
CAGGGTGGTC CAGGTGGCCC ACCGGCTCCT GCTCCGCCCC CGCCACCACC CAGTTTTGGT 
GGAGCTGCTG GAGGAGGACC ACCACCACCA GCCCCGCCAC AGATGTTCAA CGGCGCACCG 
CCGCCGCCAG CCATGGGCGG TGGACCACCG CCGGCTCCAC CGGCACCGCC AGCAATGGGT 
GGCGGACCAC CGCCAGCACC GGGCGGACCA GGGGCACCAC CACCACCGCC TCCGCCGCCG 
GGATTGGGAG GAGCGCCTAA GAAGGAAGAT CCCCAGGCAG ATCTCATGGG CTCGCTGGCC 
TCCCAGCTGC AGCAGATCAA GCTCAAAAAG AATAAGGTCA CCACCTCTGC TCCGGAGAAT 
AGCGGCAGCA GCACGTCCAG TGGCGGCAGC GGCAACTATG GAACCATAGG ACGAAGCTCT 
AATGGCATGG CCTCCATGAT GGACGAGATG GCCAAGACGC TGGCACGACG ACGCGCCCAA 
GCAGAAAAGA AGGATCCCGA TCCTGAAGCC GAAGTGAAAA AACGCCCCTG GGAAAAGTCC 
AATACACTGC CCCATAAGCT AAGCGGCGGA GCTGGCAGCG GATCTGCTGG AAGTGGCCAT 
GAAGGAGCGA ATGGTAACTC GGGCGGTGCT GGTAGCAACA CAACGAACAG TGGCGGCGAG 
TCCCCGCGAC CCATGCGCAA ACGATTCGGT AGTGCCAGCG AAGAGACCAT TCTCAAGGTC 
AATGGCGATG GCCTGTCGCT GGCGCTTTCC AATGGCGACT TGGACACACT GAAGGCTGAG 
ATTGTGCGCG AAATGCGATT GGAGATTCAG AAAGTCAAGA ACGAGATAAT AGATGCTATC 
AAATCGGAGT TTAATCGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014537.1
CDS	406..2469
Translation	MTEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSRNSLSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_001014537.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014537.3

ORF Insert Sequence:

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG GAATTCCCTC AGCGCCCCAC CGGCACCACA GCCCCAACAG 
CAGCAGCAGC AGCAGCAGGC GCAGCAAATG GGTCAGCCAG GATCCCACTA TGGACCCACT 
GGAAATGGAC CCACTTCGAA TGGCCTGCCA CAGCAGGTGA ACTCACAGAT TCCGCCGGCA 
CCGCAGCAGC AGCCACAACA GCAACAGTTT CAGCAGCAGC AGCAGCAACA GCAATATCAG 
CAAATGGTCC AGGCGGGCTA TGCGCCCTCT CAGTATCAGC AACCCCACTA CGTGCTCTCC 
AATTCTAATC CCAATCTAAC TGTGCACCAA TACCCGACAC AGCAGGCGCA GCAGCAGCCA 
CCGCAAGCGC CACAGCCGCC GCTCCAGAAC GGTGGAATGT ACATGGTGGG TCATGGTCAT 
CTACCGAGCT CCGCGAGCGC CAACAGTGTT GTGTACGCCA GCCAGCAGCA GATGCTCCCG 
CAGGCGCATC CACAGGCACC TCAGGCGCCG ACGATGCCAG GTCCGGGCTA CGGTGGTCCA 
CCAGTACCTC CGCCCCAGCA GCAGGCAGAG AATCCGTACG GTCAGGTACC CATGCCGCCG 
CCAATGAATC CGTCACAGCA ACAGCAGCCA GGTCAGGTGC CTCTAAACCG GATGAGCAGC 
CAGGGTGGTC CAGGTGGCCC ACCGGCTCCT GCTCCGCCCC CGCCACCACC CAGTTTTGGT 
GGAGCTGCTG GAGGAGGACC ACCACCACCA GCCCCGCCAC AGATGTTCAA CGGCGCACCG 
CCGCCGCCAG CCATGGGCGG TGGACCACCG CCGGCTCCAC CGGCACCGCC AGCAATGGGT 
GGCGGACCAC CGCCAGCACC GGGCGGACCA GGGGCACCAC CACCACCGCC TCCGCCGCCG 
GGATTGGGAG GAGCGCCTAA GAAGGAAGAT CCCCAGGCAG ATCTCATGGG CTCGCTGGCC 
TCCCAGCTGC AGCAGATCAA GCTCAAAAAG AATAAGGTCA CCACCTCTGC TCCGGAGAAT 
AGCGGCAGCA GCACGTCCAG TGGCGGCAGC GGCAACTATG GAACCATAGG ACGAAGCTCT 
AATGGCATGG CCTCCATGAT GGACGAGATG GCCAAGACGC TGGCACGACG ACGCGCCCAA 
GCAGAAAAGA AGGATCCCGA TCCTGAAGCC GAAGTGAAAA AACGCCCCTG GGAAAAGTCC 
AATACACTGC CCCATAAGCT AAGCGGCGGA GCTGGCAGCG GATCTGCTGG AAGTGGCCAT 
GAAGGAGCGA ATGGTAACTC GGGCGGTGCT GGTAGCAACA CAACGAACAG TGGCGGCGAG 
TCCCCGCGAC CCATGCGCAA ACGATTCGGT AGTGCCAGCG AAGAGACCAT TCTCAAGGTC 
AATGGCGATG GCCTGTCGCT GGCGCTTTCC AATGGCGACT TGGACACACT GAAGGCTGAG 
ATTGTGCGCG AAATGCGATT GGAGATTCAG AAAGTCAAGA ACGAGATAAT AGATGCTATC 
AAATCGGAGT TTAATCGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ena ( NM_001144237.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137709.1 Drosophila melanogaster enabled (ena), transcript variant F, mRNA.

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CloneID	OFa10582
Clone ID Related Accession (Same CDS sequence)	NM_001144237.2
Accession Version	NM_001144237.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144237.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGA AGAAGCTCTA CGCGAAGACC TCGTTCACCA GCAAGAAGCC ATCGAGTGCC 
GCCAATTCCA CTCCGATATT GGCCTACCAC CAGCAACAGC ATCAGCAGCC GGGCAATGGC 
ATTTGCGAGT TCCAGGTGGT GGCACCTGGT CACTCCGGTG AGCTAATGAT ACGCCGCAGC 
CAAAGTATGC ACCACAAGAT GTCGCCTCCA GTCGGCGGCC TGGGCTCCAA GTCGGAGTAC 
TACAGCATTG AGGAGCTGCA GGAGCTGGAT TTGCTGGACT ATCGTCATCC CATGTACCAT 
CATTATCAGC AGCAGGAGCT GAGGCAGCGC TACCATGAGC ACGAGCAGTT GGTGCTGCAG 
TTACCCAAGG CTACTAGTCC AAAGGCAGGA CCCATCTATG AGGCACCACA GAGATCTCAG 
CAGCAGCAGG ATCAGATGTT GTATGTACCA ACGGCAGCGC AAAGAGATTC ATCATCAAGT 
GCTGCAGCCA CATCTATAGC CAGCTCATCT ACCCTAACTT CATCCCCATC GCCATCCTCC 
TCTTCATCTC TGATCTTTTC CACTTTGCGC AAATGCGTTT CGCCCTCGAA TCCCTCGGTG 
AATCCGAACC AACCCTCAAA AACTCAACCC TCCAAACTGG GCTGTTCCAT GTCGTTTTCA 
ATTAGAACGA CAACGGCAAC AGCAGCCACA GCCGCAGCAG CAAATGCAGC AACAGCAACA 
CTATCGACAC AGCAGCAACA ACAGCAGGCA CAGCAGCAAC ACAAGCAACA TCTCTATAGC 
AACATACATC ATTATCTGAT CCGACAACAA CAGCAGAAAC AACACTACAC ATTACAACGT 
CGCCATAATT CGGTCAAAGA TAAATTCATT GGGGGCATTA CAACGATTTT TGCTGAGCAG 
AGTATTATCG GGGCGCGGGC CTCTGTGATG GTGTACGATG ACAACCAGAA GAAGTGGGTG 
CCATCGGGCA GCTCGTCGGG ATTGAGCAAG GTGCAGATCT ACCACCACCA GCAGAACAAC 
ACGTTCCGTG TGGTGGGACG AAAGCTGCAG GATCACGAGG TGGTCATCAA CTGCTCCATT 
CTGAAGGGAC TGAAGTACAA TCAGGCCACG GCAACATTTC ATCAGTGGCG CGATTCGAAA 
TTCGTTTACG GCTTGAACTT CTCGAGCCAA AATGATGCGG AGAACTTTGC TAGGGCCATG 
ATGCATGCCC TGGAAGTGCT CAGTGGTCGC GTGGCGAACA ACCCAGGTGG ACCGCCCACA 
AATGGCAATG GCTATGAGGA GGACATGGGC TATCGCACGA TGACCAGCGA GGATGCAGCC 
ATACTGCGTC AGAACAACAG CATAGGTGGG CACGTCACGC CCTCGGCCCA GACGCCCACC 
TCGCAGACCA ACCAGAATAA CATCCCCCAG AGCCCGCCGA CGCCCCAGGG TCATCATCGC 
ACCAGCAGCG CCCCACCGGC ACCACAGCCC CAACAGCAGC AGCAGCAGCA GCAGGCGCAG 
CAAATGGGTC AGCCAGGATC CCACTATGGA CCCACTGGAA ATGGACCCAC TTCGAATGGC 
CTGCCACAGC AGGTGAACTC ACAGATTCCG CCGGCACCGC AGCAGCAGCC ACAACAGCAA 
CAGTTTCAGC AGCAGCAGCA GCAACAGCAA TATCAGCAAA TGGTCCAGGC GGGCTATGCG 
CCCTCTCAGC AGTATCAGCA ACCCCACTAC GTGCTCTCCA ATTCTAATCC CAATCTAACT 
GTGCACCAAT ACCCGACACA GCAGGCGCAG CAGCAGCCAC CGCAAGCGCC ACAGCCGCCG 
CTCCAGAACG GTGGAATGTA CATGGTGGGT CATGGTCATC TACCGAGCTC CGCGAGCGCC 
AACAGTGTTG TGTACGCCAG CCAGCAGCAG ATGCTCCCGC AGGCGCATCC ACAGGCACCT 
CAGGCGCCGA CGATGCCAGG TCCGGGCTAC GGTGGTCCAC CAGTACCTCC GCCCCAGCAG 
CAGGCAGAGA ATCCGTACGG TCAGGTACCC ATGCCGCCGC CAATGAATCC GTCACAGCAA 
CAGCAGCCAG GTCAGGTGCC TCTAAACCGG ATGAGCAGCC AGGGTGGTCC AGGTGGCCCA 
CCGGCTCCTG CTCCGCCCCC GCCACCACCC AGTTTTGGTG GAGCTGCTGG AGGAGGACCA 
CCACCACCAG CCCCGCCACA GATGTTCAAC GGCGCACCGC CGCCGCCAGC CATGGGCGGT 
GGACCACCGC CGGCTCCACC GGCACCGCCA GCAATGGGTG GCGGACCACC GCCAGCACCG 
GGCGGACCAG GGGCACCACC ACCACCGCCT CCGCCGCCGG GATTGGGAGG AGCGCCTAAG 
AAGGAAGATC CCCAGGCAGA TCTCATGGGC TCGCTGGCCT CCCAGCTGCA GCAGATCAAG 
CTCAAAAAGA ATAAGGTCAC CACCTCTGCT CCGGAGAATA GCGGCAGCAG CACGTCCAGT 
GGCGGCAGCG GCAACTATGG AACCATAGGA CGAAGCTCTA ATGGCATGGC CTCCATGATG 
GACGAGATGG CCAAGACGCT GGCACGACGA CGCGCCCAAG CAGAAAAGAA GGATCCCGAT 
CCTGAAGCCG AAGTGAAAAA ACGCCCCTGG GAAAAGTCCA ATACACTGCC CCATAAGCTA 
AGCGGCGGAG CTGGCAGCGG ATCTGCTGGA AGTGGCCATG AAGGAGCGAA TGGTAACTCG 
GGCGGTGCTG GTAGCAACAC AACGAACAGT GGCGGCGAGT CCCCGCGACC CATGCGCAAA 
CGATTCGGTA GTGCCAGCGA AGAGACCATT CTCAAGGTCA ATGGCGATGG CCTGTCGCTG 
GCGCTTTCCA ATGGCGACTT GGACACACTG AAGGCTGAGA TTGTGCGCGA AATGCGATTG 
GAGATTCAGA AAGTCAAGAA CGAGATAATA GATGCTATCA AATCGGAGTT TAATCGCAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137709.1
CDS	255..3197
Translation	MAMKKLYAKTSFTSKKPSSAANSTPILAYHQQQHQQPGNGICEFQVVAPGHSGELMIRRSQSMHHKMSPPVGGLGSKSEYYSIEELQELDLLDYRHPMYHHYQQQELRQRYHEHEQLVLQLPKATSPKAGPIYEAPQRSQQQQDQMLYVPTAAQRDSSSSAAATSIASSSTLTSSPSPSSSSSLIFSTLRKCVSPSNPSVNPNQPSKTQPSKLGCSMSFSIRTTTATAATAAAANAATATLSTQQQQQQAQQQHKQHLYSNIHHYLIRQQQQKQHYTLQRRHNSVKDKFIGGITTIFAEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_001144237.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144237.2

ORF Insert Sequence:

ATGGCCATGA AGAAGCTCTA CGCGAAGACC TCGTTCACCA GCAAGAAGCC ATCGAGTGCC 
GCCAATTCCA CTCCGATATT GGCCTACCAC CAGCAACAGC ATCAGCAGCC GGGCAATGGC 
ATTTGCGAGT TCCAGGTGGT GGCACCTGGT CACTCCGGTG AGCTAATGAT ACGCCGCAGC 
CAAAGTATGC ACCACAAGAT GTCGCCTCCA GTCGGCGGCC TGGGCTCCAA GTCGGAGTAC 
TACAGCATTG AGGAGCTGCA GGAGCTGGAT TTGCTGGACT ATCGTCATCC CATGTACCAT 
CATTATCAGC AGCAGGAGCT GAGGCAGCGC TACCATGAGC ACGAGCAGTT GGTGCTGCAG 
TTACCCAAGG CTACTAGTCC AAAGGCAGGA CCCATCTATG AGGCACCACA GAGATCTCAG 
CAGCAGCAGG ATCAGATGTT GTATGTACCA ACGGCAGCGC AAAGAGATTC ATCATCAAGT 
GCTGCAGCCA CATCTATAGC CAGCTCATCT ACCCTAACTT CATCCCCATC GCCATCCTCC 
TCTTCATCTC TGATCTTTTC CACTTTGCGC AAATGCGTTT CGCCCTCGAA TCCCTCGGTG 
AATCCGAACC AACCCTCAAA AACTCAACCC TCCAAACTGG GCTGTTCCAT GTCGTTTTCA 
ATTAGAACGA CAACGGCAAC AGCAGCCACA GCCGCAGCAG CAAATGCAGC AACAGCAACA 
CTATCGACAC AGCAGCAACA ACAGCAGGCA CAGCAGCAAC ACAAGCAACA TCTCTATAGC 
AACATACATC ATTATCTGAT CCGACAACAA CAGCAGAAAC AACACTACAC ATTACAACGT 
CGCCATAATT CGGTCAAAGA TAAATTCATT GGGGGCATTA CAACGATTTT TGCTGAGCAG 
AGTATTATCG GGGCGCGGGC CTCTGTGATG GTGTACGATG ACAACCAGAA GAAGTGGGTG 
CCATCGGGCA GCTCGTCGGG ATTGAGCAAG GTGCAGATCT ACCACCACCA GCAGAACAAC 
ACGTTCCGTG TGGTGGGACG AAAGCTGCAG GATCACGAGG TGGTCATCAA CTGCTCCATT 
CTGAAGGGAC TGAAGTACAA TCAGGCCACG GCAACATTTC ATCAGTGGCG CGATTCGAAA 
TTCGTTTACG GCTTGAACTT CTCGAGCCAA AATGATGCGG AGAACTTTGC TAGGGCCATG 
ATGCATGCCC TGGAAGTGCT CAGTGGTCGC GTGGCGAACA ACCCAGGTGG ACCGCCCACA 
AATGGCAATG GCTATGAGGA GGACATGGGC TATCGCACGA TGACCAGCGA GGATGCAGCC 
ATACTGCGTC AGAACAACAG CATAGGTGGG CACGTCACGC CCTCGGCCCA GACGCCCACC 
TCGCAGACCA ACCAGAATAA CATCCCCCAG AGCCCGCCGA CGCCCCAGGG TCATCATCGC 
ACCAGCAGCG CCCCACCGGC ACCACAGCCC CAACAGCAGC AGCAGCAGCA GCAGGCGCAG 
CAAATGGGTC AGCCAGGATC CCACTATGGA CCCACTGGAA ATGGACCCAC TTCGAATGGC 
CTGCCACAGC AGGTGAACTC ACAGATTCCG CCGGCACCGC AGCAGCAGCC ACAACAGCAA 
CAGTTTCAGC AGCAGCAGCA GCAACAGCAA TATCAGCAAA TGGTCCAGGC GGGCTATGCG 
CCCTCTCAGC AGTATCAGCA ACCCCACTAC GTGCTCTCCA ATTCTAATCC CAATCTAACT 
GTGCACCAAT ACCCGACACA GCAGGCGCAG CAGCAGCCAC CGCAAGCGCC ACAGCCGCCG 
CTCCAGAACG GTGGAATGTA CATGGTGGGT CATGGTCATC TACCGAGCTC CGCGAGCGCC 
AACAGTGTTG TGTACGCCAG CCAGCAGCAG ATGCTCCCGC AGGCGCATCC ACAGGCACCT 
CAGGCGCCGA CGATGCCAGG TCCGGGCTAC GGTGGTCCAC CAGTACCTCC GCCCCAGCAG 
CAGGCAGAGA ATCCGTACGG TCAGGTACCC ATGCCGCCGC CAATGAATCC GTCACAGCAA 
CAGCAGCCAG GTCAGGTGCC TCTAAACCGG ATGAGCAGCC AGGGTGGTCC AGGTGGCCCA 
CCGGCTCCTG CTCCGCCCCC GCCACCACCC AGTTTTGGTG GAGCTGCTGG AGGAGGACCA 
CCACCACCAG CCCCGCCACA GATGTTCAAC GGCGCACCGC CGCCGCCAGC CATGGGCGGT 
GGACCACCGC CGGCTCCACC GGCACCGCCA GCAATGGGTG GCGGACCACC GCCAGCACCG 
GGCGGACCAG GGGCACCACC ACCACCGCCT CCGCCGCCGG GATTGGGAGG AGCGCCTAAG 
AAGGAAGATC CCCAGGCAGA TCTCATGGGC TCGCTGGCCT CCCAGCTGCA GCAGATCAAG 
CTCAAAAAGA ATAAGGTCAC CACCTCTGCT CCGGAGAATA GCGGCAGCAG CACGTCCAGT 
GGCGGCAGCG GCAACTATGG AACCATAGGA CGAAGCTCTA ATGGCATGGC CTCCATGATG 
GACGAGATGG CCAAGACGCT GGCACGACGA CGCGCCCAAG CAGAAAAGAA GGATCCCGAT 
CCTGAAGCCG AAGTGAAAAA ACGCCCCTGG GAAAAGTCCA ATACACTGCC CCATAAGCTA 
AGCGGCGGAG CTGGCAGCGG ATCTGCTGGA AGTGGCCATG AAGGAGCGAA TGGTAACTCG 
GGCGGTGCTG GTAGCAACAC AACGAACAGT GGCGGCGAGT CCCCGCGACC CATGCGCAAA 
CGATTCGGTA GTGCCAGCGA AGAGACCATT CTCAAGGTCA ATGGCGATGG CCTGTCGCTG 
GCGCTTTCCA ATGGCGACTT GGACACACTG AAGGCTGAGA TTGTGCGCGA AATGCGATTG 
GAGATTCAGA AAGTCAAGAA CGAGATAATA GATGCTATCA AATCGGAGTT TAATCGCAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ena ( NM_166331.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725859.2 Drosophila melanogaster enabled (ena), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10583
Clone ID Related Accession (Same CDS sequence)	NM_166331.3
Accession Version	NM_166331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166331.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATGA AGAAGCTCTA CGCGAAGACC TCGTTCACCA GCAAGAAGCC ATCGAGTGCC 
GCCAATTCCA CTCCGATATT GGCCTACCAC CAGCAACAGC ATCAGCAGCC GGGCAATGGC 
ATTTGCGAGT TCCAGGTGGT GGCACCTGGT CACTCCGGTG AGCTAATGAT ACGCCGCAGC 
CAAAGTATGC ACCACAAGAT GTCGCCTCCA GTCGGCGGCC TGGGCTCCAA GTCGGAGTAC 
TACAGCATTG AGGAGCTGCA GGAGCTGGAT TTGCTGGACT ATCGTCATCC CATGTACCAT 
CATTATCAGC AGCAGGAGCT GAGGCAGCGC TACCATGAGC ACGAGCAGTT GGTGCTGCAG 
TTACCCAAGG CTACTAGTCC AAAGGCAGGA CCCATCTATG AGGCACCACA GAGATCTCAG 
CAGCAGCAGG ATCAGATGTT TGAGCAGAGT ATTATCGGGG CGCGGGCCTC TGTGATGGTG 
TACGATGACA ACCAGAAGAA GTGGGTGCCA TCGGGCAGCT CGTCGGGATT GAGCAAGGTG 
CAGATCTACC ACCACCAGCA GAACAACACG TTCCGTGTGG TGGGACGAAA GCTGCAGGAT 
CACGAGGTGG TCATCAACTG CTCCATTCTG AAGGGACTGA AGTACAATCA GGCCACGGCA 
ACATTTCATC AGTGGCGCGA TTCGAAATTC GTTTACGGCT TGAACTTCTC GAGCCAAAAT 
GATGCGGAGA ACTTTGCTAG GGCCATGATG CATGCCCTGG AAGTGCTCAG TGGTCGCGTG 
GCGAACAACC CAGGTGGACC GCCCACAAAT GGCAATGGCT ATGAGGAGGA CATGGGCTAT 
CGCACGATGA CCAGCGAGGA TGCAGCCATA CTGCGTCAGA ACAACAGCAT AGGTGGGCAC 
GTCACGCCCT CGGCCCAGAC GCCCACCTCG CAGACCAACC AGAATAACAT CCCCCAGAGC 
CCGCCGACGC CCCAGGGTCA TCATCGCACC AGCAGGAATT CCCTCAGCGC CCCACCGGCA 
CCACAGCCCC AACAGCAGCA GCAGCAGCAG CAGGCGCAGC AAATGGGTCA GCCAGGATCC 
CACTATGGAC CCACTGGAAA TGGACCCACT TCGAATGGCC TGCCACAGCA GGTGAACTCA 
CAGATTCCGC CGGCACCGCA GCAGCAGCCA CAACAGCAAC AGTTTCAGCA GCAGCAGCAG 
CAACAGCAAT ATCAGCAAAT GGTCCAGGCG GGCTATGCGC CCTCTCAGCA GTATCAGCAA 
CCCCACTACG TGCTCTCCAA TTCTAATCCC AATCTAACTG TGCACCAATA CCCGACACAG 
CAGGCGCAGC AGCAGCCACC GCAAGCGCCA CAGCCGCCGC TCCAGAACGG TGGAATGTAC 
ATGGTGGGTC ATGGTCATCT ACCGAGCTCC GCGAGCGCCA ACAGTGTTGT GTACGCCAGC 
CAGCAGCAGA TGCTCCCGCA GGCGCATCCA CAGGCACCTC AGGCGCCGAC GATGCCAGGT 
CCGGGCTACG GTGGTCCACC AGTACCTCCG CCCCAGCAGC AGGCAGAGAA TCCGTACGGT 
CAGGTACCCA TGCCGCCGCC AATGAATCCG TCACAGCAAC AGCAGCCAGG TCAGGTGCCT 
CTAAACCGGA TGAGCAGCCA GGGTGGTCCA GGTGGCCCAC CGGCTCCTGC TCCGCCCCCG 
CCACCACCCA GTTTTGGTGG AGCTGCTGGA GGAGGACCAC CACCACCAGC CCCGCCACAG 
ATGTTCAACG GCGCACCGCC GCCGCCAGCC ATGGGCGGTG GACCACCGCC GGCTCCACCG 
GCACCGCCAG CAATGGGTGG CGGACCACCG CCAGCACCGG GCGGACCAGG GGCACCACCA 
CCACCGCCTC CGCCGCCGGG ATTGGGAGGA GCGCCTAAGA AGGAAGATCC CCAGGCAGAT 
CTCATGGGCT CGCTGGCCTC CCAGCTGCAG CAGATCAAGC TCAAAAAGAA TAAGGTCACC 
ACCTCTGCTC CGGAGAATAG CGGCAGCAGC ACGTCCAGTG GCGGCAGCGG CAACTATGGA 
ACCATAGGAC GAAGCTCTAA TGGCATGGCC TCCATGATGG ACGAGATGGC CAAGACGCTG 
GCACGACGAC GCGCCCAAGC AGAAAAGAAG GATCCCGATC CTGAAGCCGA AGTGAAAAAA 
CGCCCCTGGG AAAAGTCCAA TACACTGCCC CATAAGCTAA GCGGCGGAGC TGGCAGCGGA 
TCTGCTGGAA GTGGCCATGA AGGAGCGAAT GGTAACTCGG GCGGTGCTGG TAGCAACACA 
ACGAACAGTG GCGGCGAGTC CCCGCGACCC ATGCGCAAAC GATTCGGTAG TGCCAGCGAA 
GAGACCATTC TCAAGCAGGT CAATGGCGAT GGCCTGTCGC TGGCGCTTTC CAATGGCGAC 
TTGGACACAC TGAAGGCTGA GATTGTGCGC GAAATGCGAT TGGAGATTCA GAAAGTCAAG 
AACGAGATAA TAGATGCTAT CAAATCGGAG TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725859.2
CDS	255..2759
Translation	MAMKKLYAKTSFTSKKPSSAANSTPILAYHQQQHQQPGNGICEFQVVAPGHSGELMIRRSQSMHHKMSPPVGGLGSKSEYYSIEELQELDLLDYRHPMYHHYQQQELRQRYHEHEQLVLQLPKATSPKAGPIYEAPQRSQQQQDQMFEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSRNSLSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKQVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_166331.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166331.3

ORF Insert Sequence:

ATGGCCATGA AGAAGCTCTA CGCGAAGACC TCGTTCACCA GCAAGAAGCC ATCGAGTGCC 
GCCAATTCCA CTCCGATATT GGCCTACCAC CAGCAACAGC ATCAGCAGCC GGGCAATGGC 
ATTTGCGAGT TCCAGGTGGT GGCACCTGGT CACTCCGGTG AGCTAATGAT ACGCCGCAGC 
CAAAGTATGC ACCACAAGAT GTCGCCTCCA GTCGGCGGCC TGGGCTCCAA GTCGGAGTAC 
TACAGCATTG AGGAGCTGCA GGAGCTGGAT TTGCTGGACT ATCGTCATCC CATGTACCAT 
CATTATCAGC AGCAGGAGCT GAGGCAGCGC TACCATGAGC ACGAGCAGTT GGTGCTGCAG 
TTACCCAAGG CTACTAGTCC AAAGGCAGGA CCCATCTATG AGGCACCACA GAGATCTCAG 
CAGCAGCAGG ATCAGATGTT TGAGCAGAGT ATTATCGGGG CGCGGGCCTC TGTGATGGTG 
TACGATGACA ACCAGAAGAA GTGGGTGCCA TCGGGCAGCT CGTCGGGATT GAGCAAGGTG 
CAGATCTACC ACCACCAGCA GAACAACACG TTCCGTGTGG TGGGACGAAA GCTGCAGGAT 
CACGAGGTGG TCATCAACTG CTCCATTCTG AAGGGACTGA AGTACAATCA GGCCACGGCA 
ACATTTCATC AGTGGCGCGA TTCGAAATTC GTTTACGGCT TGAACTTCTC GAGCCAAAAT 
GATGCGGAGA ACTTTGCTAG GGCCATGATG CATGCCCTGG AAGTGCTCAG TGGTCGCGTG 
GCGAACAACC CAGGTGGACC GCCCACAAAT GGCAATGGCT ATGAGGAGGA CATGGGCTAT 
CGCACGATGA CCAGCGAGGA TGCAGCCATA CTGCGTCAGA ACAACAGCAT AGGTGGGCAC 
GTCACGCCCT CGGCCCAGAC GCCCACCTCG CAGACCAACC AGAATAACAT CCCCCAGAGC 
CCGCCGACGC CCCAGGGTCA TCATCGCACC AGCAGGAATT CCCTCAGCGC CCCACCGGCA 
CCACAGCCCC AACAGCAGCA GCAGCAGCAG CAGGCGCAGC AAATGGGTCA GCCAGGATCC 
CACTATGGAC CCACTGGAAA TGGACCCACT TCGAATGGCC TGCCACAGCA GGTGAACTCA 
CAGATTCCGC CGGCACCGCA GCAGCAGCCA CAACAGCAAC AGTTTCAGCA GCAGCAGCAG 
CAACAGCAAT ATCAGCAAAT GGTCCAGGCG GGCTATGCGC CCTCTCAGCA GTATCAGCAA 
CCCCACTACG TGCTCTCCAA TTCTAATCCC AATCTAACTG TGCACCAATA CCCGACACAG 
CAGGCGCAGC AGCAGCCACC GCAAGCGCCA CAGCCGCCGC TCCAGAACGG TGGAATGTAC 
ATGGTGGGTC ATGGTCATCT ACCGAGCTCC GCGAGCGCCA ACAGTGTTGT GTACGCCAGC 
CAGCAGCAGA TGCTCCCGCA GGCGCATCCA CAGGCACCTC AGGCGCCGAC GATGCCAGGT 
CCGGGCTACG GTGGTCCACC AGTACCTCCG CCCCAGCAGC AGGCAGAGAA TCCGTACGGT 
CAGGTACCCA TGCCGCCGCC AATGAATCCG TCACAGCAAC AGCAGCCAGG TCAGGTGCCT 
CTAAACCGGA TGAGCAGCCA GGGTGGTCCA GGTGGCCCAC CGGCTCCTGC TCCGCCCCCG 
CCACCACCCA GTTTTGGTGG AGCTGCTGGA GGAGGACCAC CACCACCAGC CCCGCCACAG 
ATGTTCAACG GCGCACCGCC GCCGCCAGCC ATGGGCGGTG GACCACCGCC GGCTCCACCG 
GCACCGCCAG CAATGGGTGG CGGACCACCG CCAGCACCGG GCGGACCAGG GGCACCACCA 
CCACCGCCTC CGCCGCCGGG ATTGGGAGGA GCGCCTAAGA AGGAAGATCC CCAGGCAGAT 
CTCATGGGCT CGCTGGCCTC CCAGCTGCAG CAGATCAAGC TCAAAAAGAA TAAGGTCACC 
ACCTCTGCTC CGGAGAATAG CGGCAGCAGC ACGTCCAGTG GCGGCAGCGG CAACTATGGA 
ACCATAGGAC GAAGCTCTAA TGGCATGGCC TCCATGATGG ACGAGATGGC CAAGACGCTG 
GCACGACGAC GCGCCCAAGC AGAAAAGAAG GATCCCGATC CTGAAGCCGA AGTGAAAAAA 
CGCCCCTGGG AAAAGTCCAA TACACTGCCC CATAAGCTAA GCGGCGGAGC TGGCAGCGGA 
TCTGCTGGAA GTGGCCATGA AGGAGCGAAT GGTAACTCGG GCGGTGCTGG TAGCAACACA 
ACGAACAGTG GCGGCGAGTC CCCGCGACCC ATGCGCAAAC GATTCGGTAG TGCCAGCGAA 
GAGACCATTC TCAAGCAGGT CAATGGCGAT GGCCTGTCGC TGGCGCTTTC CAATGGCGAC 
TTGGACACAC TGAAGGCTGA GATTGTGCGC GAAATGCGAT TGGAGATTCA GAAAGTCAAG 
AACGAGATAA TAGATGCTAT CAAATCGGAG TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ena ( NM_001299673.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286602.1 Drosophila melanogaster enabled (ena), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa21863
Clone ID Related Accession (Same CDS sequence)	NM_001299673.1
Accession Version	NM_001299673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enabled, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG GAATTCCCTC AGCGCCCCAC CGGCACCACA GCCCCAACAG 
CAGCAGCAGC AGCAGCAGGC GCAGCAAATG GGTCAGCCAG GATCCCACTA TGGACCCACT 
GGAAATGGAC CCACTTCGAA TGGCCTGCCA CAGCAGGTGA ACTCACAGAT TCCGCCGGCA 
CCGCAGCAGC AGCCACAACA GCAACAGTTT CAGCAGCAGC AGCAGCAACA GCAATATCAG 
CAAATGGTCC AGGCGGGCTA TGCGCCCTCT CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286602.1
CDS	406..2400
Translation	MTEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSRNSLSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR

Target ORF information:

RefSeq Version	NM_001299673.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enabled (ena), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299673.1

ORF Insert Sequence:

ATGACTGAGC AGAGTATTAT CGGGGCGCGG GCCTCTGTGA TGGTGTACGA TGACAACCAG 
AAGAAGTGGG TGCCATCGGG CAGCTCGTCG GGATTGAGCA AGGTGCAGAT CTACCACCAC 
CAGCAGAACA ACACGTTCCG TGTGGTGGGA CGAAAGCTGC AGGATCACGA GGTGGTCATC 
AACTGCTCCA TTCTGAAGGG ACTGAAGTAC AATCAGGCCA CGGCAACATT TCATCAGTGG 
CGCGATTCGA AATTCGTTTA CGGCTTGAAC TTCTCGAGCC AAAATGATGC GGAGAACTTT 
GCTAGGGCCA TGATGCATGC CCTGGAAGTG CTCAGTGGTC GCGTGGCGAA CAACCCAGGT 
GGACCGCCCA CAAATGGCAA TGGCTATGAG GAGGACATGG GCTATCGCAC GATGACCAGC 
GAGGATGCAG CCATACTGCG TCAGAACAAC AGCATAGGTG GGCACGTCAC GCCCTCGGCC 
CAGACGCCCA CCTCGCAGAC CAACCAGAAT AACATCCCCC AGAGCCCGCC GACGCCCCAG 
GGTCATCATC GCACCAGCAG GAATTCCCTC AGCGCCCCAC CGGCACCACA GCCCCAACAG 
CAGCAGCAGC AGCAGCAGGC GCAGCAAATG GGTCAGCCAG GATCCCACTA TGGACCCACT 
GGAAATGGAC CCACTTCGAA TGGCCTGCCA CAGCAGGTGA ACTCACAGAT TCCGCCGGCA 
CCGCAGCAGC AGCCACAACA GCAACAGTTT CAGCAGCAGC AGCAGCAACA GCAATATCAG 
CAAATGGTCC AGGCGGGCTA TGCGCCCTCT CAGCAGGCGC AGCAGCAGCC ACCGCAAGCG 
CCACAGCCGC CGCTCCAGAA CGGTGGAATG TACATGGTGG GTCATGGTCA TCTACCGAGC 
TCCGCGAGCG CCAACAGTGT TGTGTACGCC AGCCAGCAGC AGATGCTCCC GCAGGCGCAT 
CCACAGGCAC CTCAGGCGCC GACGATGCCA GGTCCGGGCT ACGGTGGTCC ACCAGTACCT 
CCGCCCCAGC AGCAGGCAGA GAATCCGTAC GGTCAGGTAC CCATGCCGCC GCCAATGAAT 
CCGTCACAGC AACAGCAGCC AGGTCAGGTG CCTCTAAACC GGATGAGCAG CCAGGGTGGT 
CCAGGTGGCC CACCGGCTCC TGCTCCGCCC CCGCCACCAC CCAGTTTTGG TGGAGCTGCT 
GGAGGAGGAC CACCACCACC AGCCCCGCCA CAGATGTTCA ACGGCGCACC GCCGCCGCCA 
GCCATGGGCG GTGGACCACC GCCGGCTCCA CCGGCACCGC CAGCAATGGG TGGCGGACCA 
CCGCCAGCAC CGGGCGGACC AGGGGCACCA CCACCACCGC CTCCGCCGCC GGGATTGGGA 
GGAGCGCCTA AGAAGGAAGA TCCCCAGGCA GATCTCATGG GCTCGCTGGC CTCCCAGCTG 
CAGCAGATCA AGCTCAAAAA GAATAAGGTC ACCACCTCTG CTCCGGAGAA TAGCGGCAGC 
AGCACGTCCA GTGGCGGCAG CGGCAACTAT GGAACCATAG GACGAAGCTC TAATGGCATG 
GCCTCCATGA TGGACGAGAT GGCCAAGACG CTGGCACGAC GACGCGCCCA AGCAGAAAAG 
AAGGATCCCG ATCCTGAAGC CGAAGTGAAA AAACGCCCCT GGGAAAAGTC CAATACACTG 
CCCCATAAGC TAAGCGGCGG AGCTGGCAGC GGATCTGCTG GAAGTGGCCA TGAAGGAGCG 
AATGGTAACT CGGGCGGTGC TGGTAGCAAC ACAACGAACA GTGGCGGCGA GTCCCCGCGA 
CCCATGCGCA AACGATTCGG TAGTGCCAGC GAAGAGACCA TTCTCAAGGT CAATGGCGAT 
GGCCTGTCGC TGGCGCTTTC CAATGGCGAC TTGGACACAC TGAAGGCTGA GATTGTGCGC 
GAAATGCGAT TGGAGATTCA GAAAGTCAAG AACGAGATAA TAGATGCTAT CAAATCGGAG 
TTTAATCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ena cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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