PPO2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPO2
Entrez Gene ID	35910
Full Name	Prophenoloxidase 2
Synonyms	CG8193,DmePPO2,Dmel\CG8193,PO45,ProPO45,proPO45,proPOA(3),proPo-A3
General protein information	Preferred Names Prophenoloxidase 2 Names CG8193 gene product from transcript CG8193-RA CG8193-PA PPO2-PA phenoloxidase A3 phenoloxidase subunit A3 prophenoloxidase 2 prophenoloxidase 45
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45A1-45A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_136599.4	NP_610443.1	prophenoloxidase 2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	PPO1	XP_312089.2
Drosophila melanogaster (fruit fly)	proPO45	NP_610443.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPO1 and PPO2 contribute to the bulk of melanization in the hemolymph, upon septic or clean injury. In contrast, PPO3, is expressed in lamellocytes and contributes to melanization during the encapsulation process.
Title: Drosophila innate immunity: regional and functional specialization of prophenoloxidases.

An important role for PPO1 and PPO2 in the survival to infection with Gram-positive bacteria and fungi, underlining the importance of melanization in insect host defense.
Title: Prophenoloxidase activation is required for survival to microbial infections in Drosophila.

rPPO1 and rPPO2 were expressed as apo-rPPO and became holo-PPO upon Cu(2+) binding; rPPO3 was holo-PPO immediately after expression.
Title: Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro.

biochemical properties, activation mode and in vivo role of Pro-PO3 (PPO3) are likely distinct from those of the other two Pro-PO enzymes involved in Drosophila physiology.
Title: Involvement of pro-phenoloxidase 3 in lamellocyte-mediated spontaneous melanization in Drosophila.

The following PPO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09312	NM_136599.4 Latest version!	Drosophila melanogaster prophenoloxidase 2 (PPO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPO2 ( NM_136599.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610443.1 Drosophila melanogaster prophenoloxidase 2 (PPO2), mRNA.

Price & Availability↑

CloneID	OFa09312
Clone ID Related Accession (Same CDS sequence)	NM_136599.4
Accession Version	NM_136599.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prophenoloxidase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136599.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACA AGAAGAATCT CCTCCTGCTT TTCGACCATC CCACCGAGCC AGTGTTCATG 
GACAAGGGCA AGAGGGTGAC CGTGTTCGAT GTGCCCGACT CCTTCCTGAC CGACCGCTAC 
CGACCCATCA GCAACGAGGT GCAGAGCCGC GTCGGCGACA AGGTGGAGCA GCGCGTTCCA 
GTCCGGGAGA TCTCCATTCC CGATCTGCGG ATCCCCATGT CCCTGGGCCG GGACGAGCAG 
TTCTCGCTGT TCCTGCCCAA GCACCGCAGG ATCGCCGGCC GCCTGATCGA TATATTCATG 
AACATGCGCT CCGTGGACGA CCTGCAGAGC GTGGCGGTGT ACGCCAGGGA TCGGGTCAAC 
CCGGTGCTCT TCAACTACGC CTTGTCGGTG GCGCTGCTCC ATCGTCCGGA CACCCAGGGC 
CTGGATCTGC CATCCTTCTC GCAGACTTTC CCCGACCGCT TCATCGACTC GCAGGTGATC 
CGCAAGATGC GGGAGGAGTC TTTTGTGGTG CAGCCCGGTT CCCGGATGCC CATCACCATT 
CCCAGGGACT ACACCGCCTC CGATCTGGAT CCCGAGCACC GGCTGTGGTA CTTCCGCGAG 
GATCTGGGCA TCAACCTTCA CCACTGGCAT TGGCATCTGG TCTACCCCTT CGAGGCCTCC 
GACCGCAGCA TAGTGGCCAA GGATCGTCGT GGCGAGCTCT TCTACTACAT GCACCAGCAG 
GTGATCGCCC GCTACAATGC GGAGCGGTTC AGCAACAACC TGGCCAGGGT TCTGCCCTTC 
AACAACCTGA GGGATCCCAT TGCCGAGGGC TACTTCCCCA AGATGGACTC TCTGGTAGCC 
AGCCGCGCGT GGCCACCGCG CTTCGAGAGC ACCAGGTTGA GCGATCTCAA CCGGGAGTCC 
GACCAGCTGA ATGTGGAGAT TGGAGATCTG GAGCGTTGGC GTGATCGCAT CTACGAGGCC 
ATTCACCAAG GATTTGTCAT GGATGAGCGT GGCAACCGGG TGCCATTGGA TGAGGCGACG 
GGCATTGATA CCCTCGGCAA CATGATTGAA TCATCCATTC TGTCGCCCAA CCGAGTGCTG 
TATGGTGACC TGCACAACAA CGGACACACC TTCATCTCGT ACGCCCACGA TCCGACCAGC 
AAGCACCTGG AGTCCTTTGG CGTGATGGGT GATGTGTCCA CGGCCATGCG CGACCCGGTC 
TTCTACAAGT GGCACTCGTA CATCGACCGC ATCTTCCAGG AGCACAAGTC GCGCCTGCCC 
GCCTATACCG AGAACCAACT AAACTACCCG GGCGTCTCGA TCGCGGGCAT CCAGGTGGAC 
ACCAATGGAG GTCGTCCCAA TAATCTGACC ACCTTCTGGC AGCAGTCGGA CGTGGACATG 
TCCCGTGGCT TCGACTTCCT GCCGCGAGGT AATGTCTTCG CCAGGTTCAC CCACCTGCAG 
CATCTGCCCT TCACCTATAC CATCAGTTTG AACAACGATA GTGGTGCCCA GCGTTTCGGC 
TACGTGCGCA TCTTCATGGC GCCGAAGAAC GACGAGCGTG GCCAGCCCAT GCTGATGCGA 
GATCAGCGCT CCATGATGAT CGAGCTGGAC AAGTTCGTGA CTTCGCTGAA CCCGGGACCC 
AACACCATCC GCCGCCGCTC CACGGAGTCG AGCGTGACCA TTCCGTTCGA GCGCACCTTC 
CGCAACCTGG ATGCCAATCG TCCGGCGGCC GGCACTCCGG AGGAGCTGGA GTTCAACTTC 
TGCGGCTGCG GCTGGCCCAA CCACATGCTG GTGCCAAAGG GTCTGCCCGA GGGACTGCAG 
TGCGTGCTGT TCATCATGGT GTCGAACTAT GAGAACGACA GGATTGACCA GCAATTGGTG 
GGTCGGTGCA GCGATGCCGC TTCCTACTGT GGCGTCCGTG ACCGTCTTTA CCCGGATCGC 
CAGTCCATGG GATTCCCCTT CGACAGGTTG CCCCGCAGCG GAGTCGACCG CCTGGTTAAC 
TTCCTCACGC CCAACATGAG CATCGTGGAC GTGAACATCC GCCACGAGAA CCGCACCGTG 
CAGCGCCCAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610443.1
CDS	90..2144
Translation	MADKKNLLLLFDHPTEPVFMDKGKRVTVFDVPDSFLTDRYRPISNEVQSRVGDKVEQRVPVREISIPDLRIPMSLGRDEQFSLFLPKHRRIAGRLIDIFMNMRSVDDLQSVAVYARDRVNPVLFNYALSVALLHRPDTQGLDLPSFSQTFPDRFIDSQVIRKMREESFVVQPGSRMPITIPRDYTASDLDPEHRLWYFREDLGINLHHWHWHLVYPFEASDRSIVAKDRRGELFYYMHQQVIARYNAERFSNNLARVLPFNNLRDPIAEGYFPKMDSLVASRAWPPRFESTRLSDLNRESDQLNVEIGDLERWRDRIYEAIHQGFVMDERGNRVPLDEATGIDTLGNMIESSILSPNRVLYGDLHNNGHTFISYAHDPTSKHLESFGVMGDVSTAMRDPVFYKWHSYIDRIFQEHKSRLPAYTENQLNYPGVSIAGIQVDTNGGRPNNLTTFWQQSDVDMSRGFDFLPRGNVFARFTHLQHLPFTYTISLNNDSGAQRFGYVRIFMAPKNDERGQPMLMRDQRSMMIELDKFVTSLNPGPNTIRRRSTESSVTIPFERTFRNLDANRPAAGTPEELEFNFCGCGWPNHMLVPKGLPEGLQCVLFIMVSNYENDRIDQQLVGRCSDAASYCGVRDRLYPDRQSMGFPFDRLPRSGVDRLVNFLTPNMSIVDVNIRHENRTVQRPN

Target ORF information:

RefSeq Version	NM_136599.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prophenoloxidase 2 (PPO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136599.4

ORF Insert Sequence:

ATGGCCGACA AGAAGAATCT CCTCCTGCTT TTCGACCATC CCACCGAGCC AGTGTTCATG 
GACAAGGGCA AGAGGGTGAC CGTGTTCGAT GTGCCCGACT CCTTCCTGAC CGACCGCTAC 
CGACCCATCA GCAACGAGGT GCAGAGCCGC GTCGGCGACA AGGTGGAGCA GCGCGTTCCA 
GTCCGGGAGA TCTCCATTCC CGATCTGCGG ATCCCCATGT CCCTGGGCCG GGACGAGCAG 
TTCTCGCTGT TCCTGCCCAA GCACCGCAGG ATCGCCGGCC GCCTGATCGA TATATTCATG 
AACATGCGCT CCGTGGACGA CCTGCAGAGC GTGGCGGTGT ACGCCAGGGA TCGGGTCAAC 
CCGGTGCTCT TCAACTACGC CTTGTCGGTG GCGCTGCTCC ATCGTCCGGA CACCCAGGGC 
CTGGATCTGC CATCCTTCTC GCAGACTTTC CCCGACCGCT TCATCGACTC GCAGGTGATC 
CGCAAGATGC GGGAGGAGTC TTTTGTGGTG CAGCCCGGTT CCCGGATGCC CATCACCATT 
CCCAGGGACT ACACCGCCTC CGATCTGGAT CCCGAGCACC GGCTGTGGTA CTTCCGCGAG 
GATCTGGGCA TCAACCTTCA CCACTGGCAT TGGCATCTGG TCTACCCCTT CGAGGCCTCC 
GACCGCAGCA TAGTGGCCAA GGATCGTCGT GGCGAGCTCT TCTACTACAT GCACCAGCAG 
GTGATCGCCC GCTACAATGC GGAGCGGTTC AGCAACAACC TGGCCAGGGT TCTGCCCTTC 
AACAACCTGA GGGATCCCAT TGCCGAGGGC TACTTCCCCA AGATGGACTC TCTGGTAGCC 
AGCCGCGCGT GGCCACCGCG CTTCGAGAGC ACCAGGTTGA GCGATCTCAA CCGGGAGTCC 
GACCAGCTGA ATGTGGAGAT TGGAGATCTG GAGCGTTGGC GTGATCGCAT CTACGAGGCC 
ATTCACCAAG GATTTGTCAT GGATGAGCGT GGCAACCGGG TGCCATTGGA TGAGGCGACG 
GGCATTGATA CCCTCGGCAA CATGATTGAA TCATCCATTC TGTCGCCCAA CCGAGTGCTG 
TATGGTGACC TGCACAACAA CGGACACACC TTCATCTCGT ACGCCCACGA TCCGACCAGC 
AAGCACCTGG AGTCCTTTGG CGTGATGGGT GATGTGTCCA CGGCCATGCG CGACCCGGTC 
TTCTACAAGT GGCACTCGTA CATCGACCGC ATCTTCCAGG AGCACAAGTC GCGCCTGCCC 
GCCTATACCG AGAACCAACT AAACTACCCG GGCGTCTCGA TCGCGGGCAT CCAGGTGGAC 
ACCAATGGAG GTCGTCCCAA TAATCTGACC ACCTTCTGGC AGCAGTCGGA CGTGGACATG 
TCCCGTGGCT TCGACTTCCT GCCGCGAGGT AATGTCTTCG CCAGGTTCAC CCACCTGCAG 
CATCTGCCCT TCACCTATAC CATCAGTTTG AACAACGATA GTGGTGCCCA GCGTTTCGGC 
TACGTGCGCA TCTTCATGGC GCCGAAGAAC GACGAGCGTG GCCAGCCCAT GCTGATGCGA 
GATCAGCGCT CCATGATGAT CGAGCTGGAC AAGTTCGTGA CTTCGCTGAA CCCGGGACCC 
AACACCATCC GCCGCCGCTC CACGGAGTCG AGCGTGACCA TTCCGTTCGA GCGCACCTTC 
CGCAACCTGG ATGCCAATCG TCCGGCGGCC GGCACTCCGG AGGAGCTGGA GTTCAACTTC 
TGCGGCTGCG GCTGGCCCAA CCACATGCTG GTGCCAAAGG GTCTGCCCGA GGGACTGCAG 
TGCGTGCTGT TCATCATGGT GTCGAACTAT GAGAACGACA GGATTGACCA GCAATTGGTG 
GGTCGGTGCA GCGATGCCGC TTCCTACTGT GGCGTCCGTG ACCGTCTTTA CCCGGATCGC 
CAGTCCATGG GATTCCCCTT CGACAGGTTG CCCCGCAGCG GAGTCGACCG CCTGGTTAAC 
TTCCTCACGC CCAACATGAG CATCGTGGAC GTGAACATCC GCCACGAGAA CCGCACCGTG 
CAGCGCCCAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PPO2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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