coil cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	coil
Entrez Gene ID	35785
Full Name	coilin
Synonyms	CG8710,Coilin,Dmel\CG8710
General protein information	Preferred Names coilin Names CG8710 gene product from transcript CG8710-RE CG8710-PC CG8710-PD CG8710-PE coil-PC coil-PD coil-PE dcoilin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44B3-44B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299255.1	NP_001286184.1	coilin, isoform E
NM_001014506.3	NP_001014506.1	coilin, isoform C
NM_001014505.2	NP_001014505.1	coilin, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coiled, a member of the leukocyte antigen 6 (Ly6) family, is crucial for septate junction formation and blood-brain barrier integrity.
Title: The CD59 family member Leaky/Coiled is required for the establishment of the blood-brain barrier in Drosophila.

coilin is required for normal Cajal body organization in Drosophila but is not essential for viability or production of functional gametes
Title: Coilin is essential for Cajal body organization in Drosophila melanogaster.

The following coil gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the coil cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09255	NM_001014505.2 Latest version!	Drosophila melanogaster coilin (coil), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09256	NM_001014506.3 Latest version!	Drosophila melanogaster coilin (coil), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21705	NM_001299255.1 Latest version!	Drosophila melanogaster coilin (coil), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

coil ( NM_001014505.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014505.1 Drosophila melanogaster coilin (coil), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa09255
Clone ID Related Accession (Same CDS sequence)	NM_001014505.2
Accession Version	NM_001014505.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	coilin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_001014505.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACACT CCAGCATGAA GGTGGATCTA TCAAACTTTT TCAAGGACGA ACGACGAAAT 
TCGTTGGTTT TCATCGATGC AGCATGGAAC AACATCAAGG ACCTCCAGGA CCACATACAA 
AACCTCTTCA GCTTGAAGGA CATTAGTCTG CTCACCTCAG ATGGATGCTA TCTGCCCCCG 
AGGGAGTCTA TCAAAGTGTT GAACTCTGCC GAAGGACTCA AAGCCTTTCG GTTTGCCAGC 
CACGATAGTG ATACCTTTGT GAGCCCTGCT CCTGTAAAAT CCAGTAAAAA GCGCAAGAAT 
CGATCCGTCG AGGAACAGGT TCACCTAACC GCCTCCACTC CCCTCCGACC TTCAAAGCGC 
TCCAAGAACC AGAACAACTC AGAATGGATA AATATTGCAG AAAACCCATC TCGTGTGAGA 
AAGAAGGAGT TGCTAGATAT GGCTCCTGGA CCTTCTGTAC AATCAAAGCT CTTGACAAAC 
AAAGGCACTC CAAAGGCCCC TGAAACTCAG ACAGAAGTTT CTAACATGTC AGCGAACATC 
GAGACAGAGA ACAAGGAATC TGCTCCTCAG ATTAAAAATA AATCCAAAAA CAAGAAACCG 
ACCAAATCGC CCGAGGCATC TGACCAGGTA GAGAATGAAC CTGCTCCTAA GAGTATTTCT 
CGATGCACGC TCAAGGAGGG TAAAATGTCA GAAAGCAAGA ATCAAGAGAC ATCGCCTGAC 
ATTCTATCAG AAAAATCTGG TGTGGTAACG AAGGAAAACG AAACTCGAGA GGAGCAACAG 
GACAAAACAC ACTTGGAGTC CAACAAAATT CCGGATAAAC TATCTCAACT GAAAGCAGGG 
GACCAAATTG AGAAGAGTCC TGGCATTGCT GCTAGCCTCC TATCGATTTC GTTCCGCTCC 
CCTCTCTTGG AGATGCCCTT TAATGTTCCG CGCATATTTC AATTTCCCAC TAAAAAACAA 
CAAATTGAAA TTCTGGAATA TAAAAAACTT AAGCCCATCA GTCCTCGTTT TTTGCTGCAG 
AAAGGAGCTA AGAGTGATGA TACGGCTAAA CAATTCCCAA GCAATGGGAA GGATTCAACC 
TTAAAGCCTA AATCCTACGA AATTTTGCAC GACGAAGAAT TACCAGATGT AAAGGATAAA 
AAAAATGTTT CAGAAGGTAT AAAGAGAGCA GTTGCACCTC TTTGCGAAGA CATCATTGAA 
ACATCAACCA CTCTTCCGGG GGCCATAGGA GCAGTTGAAT CCGCTTATCT GGACAATTCG 
ACTGAAGCTG AGACCACTCT TCCGTCCGAA GCTGAAGCAA CTAATCCACT TGAACTTACT 
GAGTCATTTC TACAGAACAA CACATCTATG GAAAAAACAC CCAAAGTCGA AAAAATTTTG 
CCAGATGACG GAAGTGCATC TCCGATTAAA AATAACGTTG ATTCGAAGGA TGTTAAAACG 
GTAACAGTGC CCATTTTTGA GGAACAACTA GTATCCGATT CCGACGACGA TGTGATGTTG 
GTAGACGACT CCAATATCGA CGTTTCATAC GGCGACTCTG ATATTGAACC GATTCCAGTA 
GTAGAAAATA GGCAATCTTT GGATATTATA AGGGACCTAC TGCGGACTGC AACTCCACTC 
AATAGCCTGC CGTCCAGGGG CGATACGGTT ATATTTAAAC TGCTGAAGAT TAAGGGAAAC 
GCCAATTCTG GCACAACCGA ATTTGTCGCC GGGAGGTGCA CTTACGTTAA CCGGCGCACC 
AAAATTGTCA CAGTGGAAAC AATAACTTAT CCCCCCGAAA TCGGTCGCAT GTTGCGTCAA 
TATTATATGA GTGGGTTGGA CGAGTCTTCT GAGGATGTGC GAACTTTAAG TATTCATCTC 
AAAGATATGC TTGAAGCCAA AATCATTGTA GCCACAATTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014505.1
CDS	44..1948
Translation	MQHSSMKVDLSNFFKDERRNSLVFIDAAWNNIKDLQDHIQNLFSLKDISLLTSDGCYLPPRESIKVLNSAEGLKAFRFASHDSDTFVSPAPVKSSKKRKNRSVEEQVHLTASTPLRPSKRSKNQNNSEWINIAENPSRVRKKELLDMAPGPSVQSKLLTNKGTPKAPETQTEVSNMSANIETENKESAPQIKNKSKNKKPTKSPEASDQVENEPAPKSISRCTLKEGKMSESKNQETSPDILSEKSGVVTKENETREEQQDKTHLESNKIPDKLSQLKAGDQIEKSPGIAASLLSISFRSPLLEMPFNVPRIFQFPTKKQQIEILEYKKLKPISPRFLLQKGAKSDDTAKQFPSNGKDSTLKPKSYEILHDEELPDVKDKKNVSEGIKRAVAPLCEDIIETSTTLPGAIGAVESAYLDNSTEAETTLPSEAEATNPLELTESFLQNNTSMEKTPKVEKILPDDGSASPIKNNVDSKDVKTVTVPIFEEQLVSDSDDDVMLVDDSNIDVSYGDSDIEPIPVVENRQSLDIIRDLLRTATPLNSLPSRGDTVIFKLLKIKGNANSGTTEFVAGRCTYVNRRTKIVTVETITYPPEIGRMLRQYYMSGLDESSEDVRTLSIHLKDMLEAKIIVATID

Target ORF information:

RefSeq Version	NM_001014505.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster coilin (coil), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014505.2

ORF Insert Sequence:

ATGCAACACT CCAGCATGAA GGTGGATCTA TCAAACTTTT TCAAGGACGA ACGACGAAAT 
TCGTTGGTTT TCATCGATGC AGCATGGAAC AACATCAAGG ACCTCCAGGA CCACATACAA 
AACCTCTTCA GCTTGAAGGA CATTAGTCTG CTCACCTCAG ATGGATGCTA TCTGCCCCCG 
AGGGAGTCTA TCAAAGTGTT GAACTCTGCC GAAGGACTCA AAGCCTTTCG GTTTGCCAGC 
CACGATAGTG ATACCTTTGT GAGCCCTGCT CCTGTAAAAT CCAGTAAAAA GCGCAAGAAT 
CGATCCGTCG AGGAACAGGT TCACCTAACC GCCTCCACTC CCCTCCGACC TTCAAAGCGC 
TCCAAGAACC AGAACAACTC AGAATGGATA AATATTGCAG AAAACCCATC TCGTGTGAGA 
AAGAAGGAGT TGCTAGATAT GGCTCCTGGA CCTTCTGTAC AATCAAAGCT CTTGACAAAC 
AAAGGCACTC CAAAGGCCCC TGAAACTCAG ACAGAAGTTT CTAACATGTC AGCGAACATC 
GAGACAGAGA ACAAGGAATC TGCTCCTCAG ATTAAAAATA AATCCAAAAA CAAGAAACCG 
ACCAAATCGC CCGAGGCATC TGACCAGGTA GAGAATGAAC CTGCTCCTAA GAGTATTTCT 
CGATGCACGC TCAAGGAGGG TAAAATGTCA GAAAGCAAGA ATCAAGAGAC ATCGCCTGAC 
ATTCTATCAG AAAAATCTGG TGTGGTAACG AAGGAAAACG AAACTCGAGA GGAGCAACAG 
GACAAAACAC ACTTGGAGTC CAACAAAATT CCGGATAAAC TATCTCAACT GAAAGCAGGG 
GACCAAATTG AGAAGAGTCC TGGCATTGCT GCTAGCCTCC TATCGATTTC GTTCCGCTCC 
CCTCTCTTGG AGATGCCCTT TAATGTTCCG CGCATATTTC AATTTCCCAC TAAAAAACAA 
CAAATTGAAA TTCTGGAATA TAAAAAACTT AAGCCCATCA GTCCTCGTTT TTTGCTGCAG 
AAAGGAGCTA AGAGTGATGA TACGGCTAAA CAATTCCCAA GCAATGGGAA GGATTCAACC 
TTAAAGCCTA AATCCTACGA AATTTTGCAC GACGAAGAAT TACCAGATGT AAAGGATAAA 
AAAAATGTTT CAGAAGGTAT AAAGAGAGCA GTTGCACCTC TTTGCGAAGA CATCATTGAA 
ACATCAACCA CTCTTCCGGG GGCCATAGGA GCAGTTGAAT CCGCTTATCT GGACAATTCG 
ACTGAAGCTG AGACCACTCT TCCGTCCGAA GCTGAAGCAA CTAATCCACT TGAACTTACT 
GAGTCATTTC TACAGAACAA CACATCTATG GAAAAAACAC CCAAAGTCGA AAAAATTTTG 
CCAGATGACG GAAGTGCATC TCCGATTAAA AATAACGTTG ATTCGAAGGA TGTTAAAACG 
GTAACAGTGC CCATTTTTGA GGAACAACTA GTATCCGATT CCGACGACGA TGTGATGTTG 
GTAGACGACT CCAATATCGA CGTTTCATAC GGCGACTCTG ATATTGAACC GATTCCAGTA 
GTAGAAAATA GGCAATCTTT GGATATTATA AGGGACCTAC TGCGGACTGC AACTCCACTC 
AATAGCCTGC CGTCCAGGGG CGATACGGTT ATATTTAAAC TGCTGAAGAT TAAGGGAAAC 
GCCAATTCTG GCACAACCGA ATTTGTCGCC GGGAGGTGCA CTTACGTTAA CCGGCGCACC 
AAAATTGTCA CAGTGGAAAC AATAACTTAT CCCCCCGAAA TCGGTCGCAT GTTGCGTCAA 
TATTATATGA GTGGGTTGGA CGAGTCTTCT GAGGATGTGC GAACTTTAAG TATTCATCTC 
AAAGATATGC TTGAAGCCAA AATCATTGTA GCCACAATTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

coil ( NM_001014506.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014506.1 Drosophila melanogaster coilin (coil), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa09256
Clone ID Related Accession (Same CDS sequence)	NM_001014506.3
Accession Version	NM_001014506.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	coilin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001014506.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCTG GACCTTCTGT ACAATCAAAG CTCTTGACAA ACAAAGGCAC TCCAAAGGCC 
CCTGAAACTC AGACAGAAGT TTCTAACATG TCAGCGAACA TCGAGACAGA GAACAAGGAA 
TCTGCTCCTC AGATTAAAAA TAAATCCAAA AACAAGAAAC CGACCAAATC GCCCGAGGCA 
TCTGACCAGG TAGAGAATGA ACCTGCTCCT AAGAGTATTT CTCGATGCAC GCTCAAGGAG 
GGTAAAATGT CAGAAAGCAA GAATCAAGAG ACATCGCCTG ACATTCTATC AGAAAAATCT 
GGTGTGGTAA CGAAGGAAAA CGAAACTCGA GAGGAGCAAC AGGACAAAAC ACACTTGGAG 
TCCAACAAAA TTCCGGATAA ACTATCTCAA CTGAAAGCAG GGGACCAAAT TGAGAAGAGT 
CCTGGCATTG CTGCTAGCCT CCTATCGATT TCGTTCCGCT CCCCTCTCTT GGAGATGCCC 
TTTAATGTTC CGCGCATATT TCAATTTCCC ACTAAAAAAC AACAAATTGA AATTCTGGAA 
TATAAAAAAC TTAAGCCCAT CAGTCCTCGT TTTTTGCTGC AGAAAGGAGC TAAGAGTGAT 
GATACGGCTA AACAATTCCC AAGCAATGGG AAGGATTCAA CCTTAAAGCC TAAATCCTAC 
GAAATTTTGC ACGACGAAGA ATTACCAGAT GTAAAGGATA AAAAAAATGT TTCAGAAGGT 
ATAAAGAGAG CAGTTGCACC TCTTTGCGAA GACATCATTG AAACATCAAC CACTCTTCCG 
GGGGCCATAG GAGCAGTTGA ATCCGCTTAT CTGGACAATT CGACTGAAGC TGAGACCACT 
CTTCCGTCCG AAGCTGAAGC AACTAATCCA CTTGAACTTA CTGAGTCATT TCTACAGAAC 
AACACATCTA TGGAAAAAAC ACCCAAAGTC GAAAAAATTT TGCCAGATGA CGGAAGTGCA 
TCTCCGATTA AAAATAACGT TGATTCGAAG GATGTTAAAA CGGTAACAGT GCCCATTTTT 
GAGGAACAAC TAGTATCCGA TTCCGACGAC GATGTGATGT TGGTAGACGA CTCCAATATC 
GACGTTTCAT ACGGCGACTC TGATATTGAA CCGATTCCAG TAGTAGAAAA TAGGCAATCT 
TTGGATATTA TAAGGGACCT ACTGCGGACT GCAACTCCAC TCAATAGCCT GCCGTCCAGG 
GGCGATACGG TTATATTTAA ACTGCTGAAG ATTAAGGGAA ACGCCAATTC TGGCACAACC 
GAATTTGTCG CCGGGAGGTG CACTTACGTT AACCGGCGCA CCAAAATTGT CACAGTGGAA 
ACAATAACTT ATCCCCCCGA AATCGGTCGC ATGTTGCGTC AATATTATAT GAGTGGGTTG 
GACGAGTCTT CTGAGGATGT GCGAACTTTA AGTATTCATC TCAAAGATAT GCTTGAAGCC 
AAAATCATTG TAGCCACAAT TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014506.1
CDS	536..2002
Translation	MAPGPSVQSKLLTNKGTPKAPETQTEVSNMSANIETENKESAPQIKNKSKNKKPTKSPEASDQVENEPAPKSISRCTLKEGKMSESKNQETSPDILSEKSGVVTKENETREEQQDKTHLESNKIPDKLSQLKAGDQIEKSPGIAASLLSISFRSPLLEMPFNVPRIFQFPTKKQQIEILEYKKLKPISPRFLLQKGAKSDDTAKQFPSNGKDSTLKPKSYEILHDEELPDVKDKKNVSEGIKRAVAPLCEDIIETSTTLPGAIGAVESAYLDNSTEAETTLPSEAEATNPLELTESFLQNNTSMEKTPKVEKILPDDGSASPIKNNVDSKDVKTVTVPIFEEQLVSDSDDDVMLVDDSNIDVSYGDSDIEPIPVVENRQSLDIIRDLLRTATPLNSLPSRGDTVIFKLLKIKGNANSGTTEFVAGRCTYVNRRTKIVTVETITYPPEIGRMLRQYYMSGLDESSEDVRTLSIHLKDMLEAKIIVATID

Target ORF information:

RefSeq Version	NM_001014506.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster coilin (coil), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014506.3

ORF Insert Sequence:

ATGGCTCCTG GACCTTCTGT ACAATCAAAG CTCTTGACAA ACAAAGGCAC TCCAAAGGCC 
CCTGAAACTC AGACAGAAGT TTCTAACATG TCAGCGAACA TCGAGACAGA GAACAAGGAA 
TCTGCTCCTC AGATTAAAAA TAAATCCAAA AACAAGAAAC CGACCAAATC GCCCGAGGCA 
TCTGACCAGG TAGAGAATGA ACCTGCTCCT AAGAGTATTT CTCGATGCAC GCTCAAGGAG 
GGTAAAATGT CAGAAAGCAA GAATCAAGAG ACATCGCCTG ACATTCTATC AGAAAAATCT 
GGTGTGGTAA CGAAGGAAAA CGAAACTCGA GAGGAGCAAC AGGACAAAAC ACACTTGGAG 
TCCAACAAAA TTCCGGATAA ACTATCTCAA CTGAAAGCAG GGGACCAAAT TGAGAAGAGT 
CCTGGCATTG CTGCTAGCCT CCTATCGATT TCGTTCCGCT CCCCTCTCTT GGAGATGCCC 
TTTAATGTTC CGCGCATATT TCAATTTCCC ACTAAAAAAC AACAAATTGA AATTCTGGAA 
TATAAAAAAC TTAAGCCCAT CAGTCCTCGT TTTTTGCTGC AGAAAGGAGC TAAGAGTGAT 
GATACGGCTA AACAATTCCC AAGCAATGGG AAGGATTCAA CCTTAAAGCC TAAATCCTAC 
GAAATTTTGC ACGACGAAGA ATTACCAGAT GTAAAGGATA AAAAAAATGT TTCAGAAGGT 
ATAAAGAGAG CAGTTGCACC TCTTTGCGAA GACATCATTG AAACATCAAC CACTCTTCCG 
GGGGCCATAG GAGCAGTTGA ATCCGCTTAT CTGGACAATT CGACTGAAGC TGAGACCACT 
CTTCCGTCCG AAGCTGAAGC AACTAATCCA CTTGAACTTA CTGAGTCATT TCTACAGAAC 
AACACATCTA TGGAAAAAAC ACCCAAAGTC GAAAAAATTT TGCCAGATGA CGGAAGTGCA 
TCTCCGATTA AAAATAACGT TGATTCGAAG GATGTTAAAA CGGTAACAGT GCCCATTTTT 
GAGGAACAAC TAGTATCCGA TTCCGACGAC GATGTGATGT TGGTAGACGA CTCCAATATC 
GACGTTTCAT ACGGCGACTC TGATATTGAA CCGATTCCAG TAGTAGAAAA TAGGCAATCT 
TTGGATATTA TAAGGGACCT ACTGCGGACT GCAACTCCAC TCAATAGCCT GCCGTCCAGG 
GGCGATACGG TTATATTTAA ACTGCTGAAG ATTAAGGGAA ACGCCAATTC TGGCACAACC 
GAATTTGTCG CCGGGAGGTG CACTTACGTT AACCGGCGCA CCAAAATTGT CACAGTGGAA 
ACAATAACTT ATCCCCCCGA AATCGGTCGC ATGTTGCGTC AATATTATAT GAGTGGGTTG 
GACGAGTCTT CTGAGGATGT GCGAACTTTA AGTATTCATC TCAAAGATAT GCTTGAAGCC 
AAAATCATTG TAGCCACAAT TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

coil ( NM_001299255.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286184.1 Drosophila melanogaster coilin (coil), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa21705
Clone ID Related Accession (Same CDS sequence)	NM_001299255.1
Accession Version	NM_001299255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	coilin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACACT CCAGCATGAA GGTGGATCTA TCAAACTTTT TCAAGGACGA ACGACGAAAT 
TCGTTGGTTT TCATCGATGC AGCATGGAAC AACATCAAGG ACCTCCAGGA CCACATACAA 
AACCTCTTCA GCTTGAAGGA CATTAGTCTG CTCACCTCAG ATGGATGCTA TCTGCCCCCG 
AGGGAGTCTA TCAAAGTGTT GAACTCTGCC GAAGGACTCA AAGCCTTTCG GTTTGCCAGC 
CACGATAGTG ATACCTTTGT GAGCCCTGCT CCTGTAAAAT CCAGTAAAAA GCGCAAGAAT 
CGATCCGTCG AGGAACAGGT TCACCTAACC GCCTCCACTC CCCTCCGACC TTCAAAGCGC 
TCCAAGAACC AGAACAACTC AGAATGGATA AATATTGCAG AAAACCCATC TCGTGTGAGA 
AAGAAGGAGT TGCTAGATAT GGCTCCTGGA CCTTCTGTAC AATCAAAGCT CTTGACAAAC 
AAAGGCACTC CAAAGGCCCC TGAAACTCAG ACAGAAGTTT CTAACATGTC AGCGAACATC 
GAGACAGAGA ACAAGGAATC TGCTCCTCAG ATTAAAAATA AATCCAAAAA CAAGAAACCG 
ACCAAATCGC CCGAGGCATC TGACCAGGTA GAGAATGAAC CTGCTCCTAA GAGTATTTCT 
CGATGCACGC TCAAGGAGGG TAAAATGTCA GAAAGCAAGA ATCAAGAGAC ATCGCCTGAC 
ATTCTATCAG AAAAATCTGG TGTGGTAACG AAGGAAAACG AAACTCGAGA GGAGCAACAG 
GACAAAACAC ACTTGGAGTC CAACAAAATT CCGGATAAAC TATCTCAACT GAAAGCAGGG 
GACCAAATTG AGAAGAGTCC TGGCATTGCT GCTAGCCTCC TATCGATTTC GTTCCGCTCC 
CCTCTCTTGG AGATGCCCTT TAATGTTCCG CGCATATTTC AATTTCCCAC TAAAAAACAA 
CAAATTGAAA TTCTGGAATA TAAAAAACTT AAGCCCATCA GTCCTCGTTT TTTGCTGCAG 
AAAGGAGCTA AGAGTGATGA TACGGCTAAA CAATTCCCAA GCAATGGGAA GGATTCAACC 
TTAAAGCCTA AATCCTACGA AATTTTGCAC GACGAAGAAT TACCAGATGT AAAGGATAAA 
AAAAATGTTT CAGAAGGTAT AAAGAGAGCA GTTGCACCTC TTTGCGAAGA CATCATTGAA 
ACATCAACCA CTCTTCCGGG GGCCATAGGA GCAGTTGAAT CCGCTTATCT GGACAATTCG 
ACTGAAGCTG AGACCACTCT TCCGTCCGAA GCTGAAGCAA CTAATCCACT TGAACTTACT 
GAGTCATTTC TACAGAACAA CACATCTATG GAAAAAACAC CCAAAGTCGA AAAAATTTTG 
CCAGATGACG GAAGTGCATC TCCGATTAAA AATAACGTTG ATTCGAAGGA TGTTAAAACG 
GTAACAGTGC CCATTTTTGA GGAACAACTA GTATCCGATT CCGACGACGA TGTGATGTTG 
GTAGACGACT CCAATATCGA CGTTTCATAC GGCGACTCTG ATATTGAACC GATTCCAGTA 
GAAAATAGGC AATCTTTGGA TATTATAAGG GACCTACTGC GGACTGCAAC TCCACTCAAT 
AGCCTGCCGT CCAGGGGCGA TACGGTTATA TTTAAACTGC TGAAGATTAA GGGAAACGCC 
AATTCTGGCA CAACCGAATT TGTCGCCGGG AGGTGCACTT ACGTTAACCG GCGCACCAAA 
ATTGTCACAG TGGAAACAAT AACTTATCCC CCCGAAATCG GTCGCATGTT GCGTCAATAT 
TATATGAGTG GGTTGGACGA GTCTTCTGAG GATGTGCGAA CTTTAAGTAT TCATCTCAAA 
GATATGCTTG AAGCCAAAAT CATTGTAGCC ACAATTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286184.1
CDS	44..1945
Translation	MQHSSMKVDLSNFFKDERRNSLVFIDAAWNNIKDLQDHIQNLFSLKDISLLTSDGCYLPPRESIKVLNSAEGLKAFRFASHDSDTFVSPAPVKSSKKRKNRSVEEQVHLTASTPLRPSKRSKNQNNSEWINIAENPSRVRKKELLDMAPGPSVQSKLLTNKGTPKAPETQTEVSNMSANIETENKESAPQIKNKSKNKKPTKSPEASDQVENEPAPKSISRCTLKEGKMSESKNQETSPDILSEKSGVVTKENETREEQQDKTHLESNKIPDKLSQLKAGDQIEKSPGIAASLLSISFRSPLLEMPFNVPRIFQFPTKKQQIEILEYKKLKPISPRFLLQKGAKSDDTAKQFPSNGKDSTLKPKSYEILHDEELPDVKDKKNVSEGIKRAVAPLCEDIIETSTTLPGAIGAVESAYLDNSTEAETTLPSEAEATNPLELTESFLQNNTSMEKTPKVEKILPDDGSASPIKNNVDSKDVKTVTVPIFEEQLVSDSDDDVMLVDDSNIDVSYGDSDIEPIPVENRQSLDIIRDLLRTATPLNSLPSRGDTVIFKLLKIKGNANSGTTEFVAGRCTYVNRRTKIVTVETITYPPEIGRMLRQYYMSGLDESSEDVRTLSIHLKDMLEAKIIVATID

Target ORF information:

RefSeq Version	NM_001299255.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster coilin (coil), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299255.1

ORF Insert Sequence:

ATGCAACACT CCAGCATGAA GGTGGATCTA TCAAACTTTT TCAAGGACGA ACGACGAAAT 
TCGTTGGTTT TCATCGATGC AGCATGGAAC AACATCAAGG ACCTCCAGGA CCACATACAA 
AACCTCTTCA GCTTGAAGGA CATTAGTCTG CTCACCTCAG ATGGATGCTA TCTGCCCCCG 
AGGGAGTCTA TCAAAGTGTT GAACTCTGCC GAAGGACTCA AAGCCTTTCG GTTTGCCAGC 
CACGATAGTG ATACCTTTGT GAGCCCTGCT CCTGTAAAAT CCAGTAAAAA GCGCAAGAAT 
CGATCCGTCG AGGAACAGGT TCACCTAACC GCCTCCACTC CCCTCCGACC TTCAAAGCGC 
TCCAAGAACC AGAACAACTC AGAATGGATA AATATTGCAG AAAACCCATC TCGTGTGAGA 
AAGAAGGAGT TGCTAGATAT GGCTCCTGGA CCTTCTGTAC AATCAAAGCT CTTGACAAAC 
AAAGGCACTC CAAAGGCCCC TGAAACTCAG ACAGAAGTTT CTAACATGTC AGCGAACATC 
GAGACAGAGA ACAAGGAATC TGCTCCTCAG ATTAAAAATA AATCCAAAAA CAAGAAACCG 
ACCAAATCGC CCGAGGCATC TGACCAGGTA GAGAATGAAC CTGCTCCTAA GAGTATTTCT 
CGATGCACGC TCAAGGAGGG TAAAATGTCA GAAAGCAAGA ATCAAGAGAC ATCGCCTGAC 
ATTCTATCAG AAAAATCTGG TGTGGTAACG AAGGAAAACG AAACTCGAGA GGAGCAACAG 
GACAAAACAC ACTTGGAGTC CAACAAAATT CCGGATAAAC TATCTCAACT GAAAGCAGGG 
GACCAAATTG AGAAGAGTCC TGGCATTGCT GCTAGCCTCC TATCGATTTC GTTCCGCTCC 
CCTCTCTTGG AGATGCCCTT TAATGTTCCG CGCATATTTC AATTTCCCAC TAAAAAACAA 
CAAATTGAAA TTCTGGAATA TAAAAAACTT AAGCCCATCA GTCCTCGTTT TTTGCTGCAG 
AAAGGAGCTA AGAGTGATGA TACGGCTAAA CAATTCCCAA GCAATGGGAA GGATTCAACC 
TTAAAGCCTA AATCCTACGA AATTTTGCAC GACGAAGAAT TACCAGATGT AAAGGATAAA 
AAAAATGTTT CAGAAGGTAT AAAGAGAGCA GTTGCACCTC TTTGCGAAGA CATCATTGAA 
ACATCAACCA CTCTTCCGGG GGCCATAGGA GCAGTTGAAT CCGCTTATCT GGACAATTCG 
ACTGAAGCTG AGACCACTCT TCCGTCCGAA GCTGAAGCAA CTAATCCACT TGAACTTACT 
GAGTCATTTC TACAGAACAA CACATCTATG GAAAAAACAC CCAAAGTCGA AAAAATTTTG 
CCAGATGACG GAAGTGCATC TCCGATTAAA AATAACGTTG ATTCGAAGGA TGTTAAAACG 
GTAACAGTGC CCATTTTTGA GGAACAACTA GTATCCGATT CCGACGACGA TGTGATGTTG 
GTAGACGACT CCAATATCGA CGTTTCATAC GGCGACTCTG ATATTGAACC GATTCCAGTA 
GAAAATAGGC AATCTTTGGA TATTATAAGG GACCTACTGC GGACTGCAAC TCCACTCAAT 
AGCCTGCCGT CCAGGGGCGA TACGGTTATA TTTAAACTGC TGAAGATTAA GGGAAACGCC 
AATTCTGGCA CAACCGAATT TGTCGCCGGG AGGTGCACTT ACGTTAACCG GCGCACCAAA 
ATTGTCACAG TGGAAACAAT AACTTATCCC CCCGAAATCG GTCGCATGTT GCGTCAATAT 
TATATGAGTG GGTTGGACGA GTCTTCTGAG GATGTGCGAA CTTTAAGTAT TCATCTCAAA 
GATATGCTTG AAGCCAAAAT CATTGTAGCC ACAATTGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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coil cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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