dia cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dia
Entrez Gene ID	35340
Full Name	diaphanous
Synonyms	38E.16,CG1768,DIA,Dia,Dias,Dmel\CG1768,l(2)k07135,ms(2)04138
General protein information	Preferred Names diaphanous Names CG1768 gene product from transcript CG1768-RE CG1768-PA CG1768-PB CG1768-PC CG1768-PD CG1768-PE dia-PA dia-PB dia-PC dia-PD dia-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38E7-38E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273711.1	NP_001260640.1	diaphanous, isoform E
NM_057633.5	NP_476981.1	diaphanous, isoform A
NM_165341.3	NP_724285.1	diaphanous, isoform B
NM_001259184.1	NP_001246113.1	diaphanous, isoform D
NM_001259183.1	NP_001246112.1	diaphanous, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIAPH1	NP_001073280.1
	DIAPH1	XP_001089232.2
Rattus norvegicus (Norway rat)	Diaph1	NP_001100863.2
Mus musculus (house mouse)	Diap1	NP_031884.1
Pan troglodytes (chimpanzee)	DIAPH1	XP_003310971.1
Drosophila melanogaster (fruit fly)	dia	NP_001246113.1
Canis lupus familiaris (dog)	DIAPH1	XP_005617363.1
Xenopus tropicalis (tropical clawed frog)	diaph1	XP_002940036.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using constitutively active Dia constructs, authors provide additional evidence that Dia functions upstream of SCAR activity to regulate actin dynamics at the fusion site and to localize the Abi-SCAR complex.
Title: Diaphanous regulates SCAR complex localization during Drosophila myoblast fusion.

Dia plays two roles during invasive podosome formation at the fusion site: it dictates the level of linear F-actin polymerization, and it is required for appropriate branched actin polymerization via localization of SCAR and WASp.
Title: The Formin Diaphanous Regulates Myoblast Fusion through Actin Polymerization and Arp2/3 Regulation.

Cdc42 and the formins diaphanous and DAAM play pivotal roles in heart lumen formation through the spatiotemporal regulation of the actomyosin network.
Title: Cdc42 and formin activity control non-muscle myosin dynamics during Drosophila heart morphogenesis.

EnaEVH1 binds Dia directly and inhibits DiaFH1FH2-mediated nucleation in vitro
Title: Enabled negatively regulates diaphanous-driven actin dynamics in vitro and in vivo.

Dia apical targeting also depends on binding to Rho1, which is critical for activation-induced conformational change, as well as physically anchoring Dia to the apical membrane
Title: Apical targeting of the formin Diaphanous in Drosophila tubular epithelia.

The following dia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16950	NM_001259183.1 Latest version!	Drosophila melanogaster diaphanous (dia), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa15059	NM_165341.3 Latest version!	Drosophila melanogaster diaphanous (dia), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa15059	NM_057633.5 Latest version!	Drosophila melanogaster diaphanous (dia), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa15059	NM_001273711.1 Latest version!	Drosophila melanogaster diaphanous (dia), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa16949	NM_001259184.1 Latest version!	Drosophila melanogaster diaphanous (dia), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dia ( NM_001259183.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246112.1 Drosophila melanogaster diaphanous (dia), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16950
Clone ID Related Accession (Same CDS sequence)	NM_001259183.1
Accession Version	NM_001259183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diaphanous, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTCA AAAAGCTGGG AATGTCTCGT CACGAGAAAA CGAAATCCAC GGGCGGCGGG 
CTCCTGGACA GTCTGTTCGG AAGACCCTCG AAGTCCAAGG GAGGAACCAT CAGCAGTGGC 
ACCCTGGCCC ATGGCGGACG ACCCGTGTCC GCGGACAACT ATGTGGTGCC GGGCGTGGAG 
GACTTTGAGC AGTACATCCA GCAGCTAAGC GTTGCGGAGC TGGATGCGAA GTTTCTGGAG 
ATCATCGAGG ACATGAACAT TCCGAAGGAC AAGAGGGAGC CCCTGTTGGC CAAATCGAAG 
GAGGAGCGAC AGAAGATGAT TATGTGGCAC TTGAAAGGTA AAGAACATGG TGCAAGTACA 
CGTTTTCAAA CAGAAAACCG CATTTGTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246112.1
CDS	139..531
Translation	MRLKKLGMSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQQLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKEHGASTRFQTENRICV

Target ORF information:

RefSeq Version	NM_001259183.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diaphanous (dia), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259183.1

ORF Insert Sequence:

ATGCGCCTCA AAAAGCTGGG AATGTCTCGT CACGAGAAAA CGAAATCCAC GGGCGGCGGG 
CTCCTGGACA GTCTGTTCGG AAGACCCTCG AAGTCCAAGG GAGGAACCAT CAGCAGTGGC 
ACCCTGGCCC ATGGCGGACG ACCCGTGTCC GCGGACAACT ATGTGGTGCC GGGCGTGGAG 
GACTTTGAGC AGTACATCCA GCAGCTAAGC GTTGCGGAGC TGGATGCGAA GTTTCTGGAG 
ATCATCGAGG ACATGAACAT TCCGAAGGAC AAGAGGGAGC CCCTGTTGGC CAAATCGAAG 
GAGGAGCGAC AGAAGATGAT TATGTGGCAC TTGAAAGGTA AAGAACATGG TGCAAGTACA 
CGTTTTCAAA CAGAAAACCG CATTTGTGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dia ( NM_001273711.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260640.1 Drosophila melanogaster diaphanous (dia), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa15059
Clone ID Related Accession (Same CDS sequence)	NM_001273711.1 , NM_057633.5 , NM_165341.3
Accession Version	NM_001273711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diaphanous, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGTC ACGAGAAAAC GAAATCCACG GGCGGCGGGC TCCTGGACAG TCTGTTCGGA 
AGACCCTCGA AGTCCAAGGG AGGAACCATC AGCAGTGGCA CCCTGGCCCA TGGCGGACGA 
CCCGTGTCCG CGGACAACTA TGTGGTGCCG GGCGTGGAGG ACTTTGAGCA GTACATCCAG 
CAGCTAAGCG TTGCGGAGCT GGATGCGAAG TTTCTGGAGA TCATCGAGGA CATGAACATT 
CCGAAGGACA AGAGGGAGCC CCTGTTGGCC AAATCGAAGG AGGAGCGACA GAAGATGATT 
ATGTGGCACT TGAAAGGTAA AAACTCACTG GAGCGTAGCG CCAACTCCCG CTTCGAGAAG 
CCCATAGACT ATGTGGAATA CCTGCAGAAT GGGGAGCACA GCACGCACAA GGTGTACCAA 
TGTGTGGAAT CTCTGCGCGT GGCGCTCACC AGCAATCCGA TCTCGTGGAT CAAGGAGTTT 
GGAGTGGCGG GCATCGGGAC GATTGAGAAG CTGCTGGCCC GGTCAAAGAA TAATGCCAGC 
TACGAGAAGA TCGAGTTCGA GGCGATTCGG TGCCTGAAGG CGATCATGAA CAACACATGG 
GGTCTGAACG TGGTGCTCAA TCCGGATCAG CATAGTGTGG TGCTGCTGCT GGCGCAATCC 
CTGGATCCCC GGAAGCCGCA GACGATGTGT GAAGCCCTCA AGCTGCTGGC CTCGTTCTGC 
ATTGTCTATG AGCGGAATGG CTACGAGAAG GTTCTCCGAG CCATAACCAC TATTGCAGCC 
ACATCCTTTA AAGCGAGCGA GCGCTTTCGA CCCATAGTGG ATGCCTTGTT TGCATCGGAT 
CAGCAGGATC CCAAACGGGA CTTGGCATGC CACAGCCTGA TTTTTATTAA TACTCTCACC 
AATACCCCCA CGGATTTGAA CTTCCGCCTG CACTTGCGAT GTGAGATTAT GCGCATGGGT 
CTATACGATC GCCTAGATGA GTTCACCAAA ATCGTGGAGG CCAGCAATAA TGAGAACCTG 
CAGCAGCACT TCAAGATCTT CAACGAGATC CGCGAGGATG ACTTCGAGGA GTTTGTGCAG 
CGCTTCGATA ATGTCACCTT CAACATGGAC GACGCCACCG ATTGCTTCGA TGTGCTGAAG 
AACCTGGTGA CTGACACCAC TTCCGAGCCC TACTTCCTGT CCATCCTGCA GCATTTGCTG 
TACATCAGGG ATGACTTCTA CTTCCGACCT GCCTATTATC AGCTGATTGA GGAGTGCATC 
TCACAAATCG TCTTCCACAA GGGTTACTGT GATCCGAATT TCGAGAACCG GAACTTTAAT 
ATAGACACCT CGCTACTGCT GGACGACATT GTGGAGAAGG CCAAGGCCAA GGAGTCGAAG 
CGGTCGGAGG AGTACGAGAA GAAGATCGAG CAGCTGGAGA GTGCCAAGCA AGAGGCGGAA 
GCGAAGGCGG CTCATCTGGA GGAGAAAGTC AAGTTGATGG AGGCTAATGG TGTGGCGGCT 
CCGTCGCCCA ATAAGCTACC CAAGGTAAAC ATACCCATGC CACCGCCACC ACCAGGAGGA 
GGAGGAGCAC CTCCCCCACC GCCGCCACCT ATGCCGGGAC GAGCAGGTGG TGGACCTCCG 
CCTCCACCAC CACCTCCCAT GCCGGGAAGG GCAGGTGGAC CGCCACCACC TCCTCCACCG 
CCCGGAATGG GAGGCCCACC GCCTCCACCC ATGCCTGGCA TGATGCGCCC AGGAGGGGGA 
CCTCCACCAC CGCCCATGAT GATGGGGCCC ATGGTTCCCG TTCTGCCTCA TGGCTTAAAA 
CCGAAGAAGA AGTGGGACGT CAAGAATCCC ATGAAGCGGG CCAACTGGAA AGCCATTGTC 
CCGGCCAAAA TGTCCGACAA GGCATTCTGG GTCAAGTGCC AGGAGGATAA GCTGGCCCAG 
GATGACTTCC TCGCAGAACT GGCCGTGAAA TTCTCTTCTA AACCTGTAAA GAAAGAACAG 
AAGGATGCGG TGGACAAGCC AACGACGCTG ACAAAGAAAA ATGTCGATCT TCGTGTGCTC 
GACTCGAAGA CTGCTCAGAA CTTGGCTATT ATGTTGGGAG GATCGCTGAA GCATCTGTCC 
TACGAACAGA TCAAGATCTG CTTGCTGCGC TGCGACACCG ACATCCTGTC CTCCAATATC 
CTGCAGCAAC TTATCCAGTA CCTTCCGCCG CCAGAGCACC TCAAGCGTTT GCAGGAGATC 
AAGGCAAAGG GCGAACCGCT ACCGCCGATT GAACAGTTTG CAGCCACAAT AGGGGAAATT 
AAACGCCTTT CGCCGCGACT TCACAACCTG AACTTCAAGC TGACCTACGC GGACATGGTG 
CAGGATATCA AACCCGACAT TGTGGCAGGA ACGGCAGCAT GCGAAGAGAT CCGGAATAGC 
AAAAAGTTCT CAAAGATCTT GGAGCTGATT CTGCTGCTTG GAAATTACAT GAACTCGGGC 
TCCAAAAACG AGGCCGCCTT TGGCTTTGAG ATCAGTTATT TAACCAAACT GTCCAATACG 
AAGGATGCGG ATAATAAGCA GACATTGCTG CACTACCTGG CTGACCTGGT GGAAAAGAAA 
TTCCCAGATG CACTAAACTT CTACGACGAT CTGTCGCATG TTAATAAAGC GTCGCGGGTC 
AACATGGATG CCATCCAAAA GGCCATGCGG CAAATGAATT CGGCGGTTAA GAACCTGGAA 
ACTGATCTCC AGAACAACAA GGTGCCGCAG TGTGATGATG ACAAGTTTAG CGAGGTGATG 
GGCAAGTTTG CCGAGGAGTG CAGACAACAA GTGGACGTGC TGGGCAAAAT GCAGCTGCAA 
ATGGAGAAGC TGTACAAGGA CCTCAGCGAG TACTATGCCT TCGATCCCAG CAAATACACA 
ATGGAGGAGT TCTTTGCGGA CATCAAGACT TTCAAGGATG CCTTCCAAGC GGCCCACAAC 
GACAATGTCC GGGTACGCGA GGAGCTGGAG AAGAAGCGTC GTCTGCAGGA GGCCCGAGAG 
CAGTCTGCTC GAGAGCAACA GGAGCGCCAA CAGCGTAAGA AGGCAGTGGT TGACATGGAT 
GCCCCGCAGA CGCAGGAAGG CGTGATGGAC AGTCTGTTGG AGGCGCTGCA AACGGGCTCA 
GCCTTTGGCC AACGAAATCG ACAGGCCCGG CGACAACGAC CGGCGGGAGC GGAGCGGAGG 
GCACAGCTCA GCCGGAGTCG ATCGCGGACG CGTGTCACCA ACGGACAACT AATGACCCGC 
GAAATGATCC TCAACGAGGT TCTAGGCTCC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260640.1
CDS	522..3797
Translation	MSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQQLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKNSLERSANSRFEKPIDYVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKNNASYEKIEFEAIRCLKAIMNNTWGLNVVLNPDQHSVVLLLAQSLDPRKPQTMCEALKLLASFCIVYERNGYEKVLRAITTIAATSFKASERFRPIVDALFASDQQDPKRDLACHSLIFINTLTNTPTDLNFRLHLRCEIMRMGLYDRLDEFTKIVEASNNENLQQHFKIFNEIREDDFEEFVQRFDNVTFNMDDATDCFDVLKNLVTDTTSEPYFLSILQHLLYIRDDFYFRPAYYQLIEECISQIVFHKGYCDPNFENRNFNIDTSLLLDDIVEKAKAKESKRSEEYEKKIEQLESAKQEAEAKAAHLEEKVKLMEANGVAAPSPNKLPKVNIPMPPPPPGGGGAPPPPPPPMPGRAGGGPPPPPPPPMPGRAGGPPPPPPPPGMGGPPPPPMPGMMRPGGGPPPPPMMMGPMVPVLPHGLKPKKKWDVKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQDDFLAELAVKFSSKPVKKEQKDAVDKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSYEQIKICLLRCDTDILSSNILQQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQFAATIGEIKRLSPRLHNLNFKLTYADMVQDIKPDIVAGTAACEEIRNSKKFSKILELILLLGNYMNSGSKNEAAFGFEISYLTKLSNTKDADNKQTLLHYLADLVEKKFPDALNFYDDLSHVNKASRVNMDAIQKAMRQMNSAVKNLETDLQNNKVPQCDDDKFSEVMGKFAEECRQQVDVLGKMQLQMEKLYKDLSEYYAFDPSKYTMEEFFADIKTFKDAFQAAHNDNVRVREELEKKRRLQEAREQSAREQQERQQRKKAVVDMDAPQTQEGVMDSLLEALQTGSAFGQRNRQARRQRPAGAERRAQLSRSRSRTRVTNGQLMTREMILNEVLGSA

Target ORF information:

RefSeq Version	NM_001273711.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diaphanous (dia), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273711.1

ORF Insert Sequence:

ATGTCTCGTC ACGAGAAAAC GAAATCCACG GGCGGCGGGC TCCTGGACAG TCTGTTCGGA 
AGACCCTCGA AGTCCAAGGG AGGAACCATC AGCAGTGGCA CCCTGGCCCA TGGCGGACGA 
CCCGTGTCCG CGGACAACTA TGTGGTGCCG GGCGTGGAGG ACTTTGAGCA GTACATCCAG 
CAGCTAAGCG TTGCGGAGCT GGATGCGAAG TTTCTGGAGA TCATCGAGGA CATGAACATT 
CCGAAGGACA AGAGGGAGCC CCTGTTGGCC AAATCGAAGG AGGAGCGACA GAAGATGATT 
ATGTGGCACT TGAAAGGTAA AAACTCACTG GAGCGTAGCG CCAACTCCCG CTTCGAGAAG 
CCCATAGACT ATGTGGAATA CCTGCAGAAT GGGGAGCACA GCACGCACAA GGTGTACCAA 
TGTGTGGAAT CTCTGCGCGT GGCGCTCACC AGCAATCCGA TCTCGTGGAT CAAGGAGTTT 
GGAGTGGCGG GCATCGGGAC GATTGAGAAG CTGCTGGCCC GGTCAAAGAA TAATGCCAGC 
TACGAGAAGA TCGAGTTCGA GGCGATTCGG TGCCTGAAGG CGATCATGAA CAACACATGG 
GGTCTGAACG TGGTGCTCAA TCCGGATCAG CATAGTGTGG TGCTGCTGCT GGCGCAATCC 
CTGGATCCCC GGAAGCCGCA GACGATGTGT GAAGCCCTCA AGCTGCTGGC CTCGTTCTGC 
ATTGTCTATG AGCGGAATGG CTACGAGAAG GTTCTCCGAG CCATAACCAC TATTGCAGCC 
ACATCCTTTA AAGCGAGCGA GCGCTTTCGA CCCATAGTGG ATGCCTTGTT TGCATCGGAT 
CAGCAGGATC CCAAACGGGA CTTGGCATGC CACAGCCTGA TTTTTATTAA TACTCTCACC 
AATACCCCCA CGGATTTGAA CTTCCGCCTG CACTTGCGAT GTGAGATTAT GCGCATGGGT 
CTATACGATC GCCTAGATGA GTTCACCAAA ATCGTGGAGG CCAGCAATAA TGAGAACCTG 
CAGCAGCACT TCAAGATCTT CAACGAGATC CGCGAGGATG ACTTCGAGGA GTTTGTGCAG 
CGCTTCGATA ATGTCACCTT CAACATGGAC GACGCCACCG ATTGCTTCGA TGTGCTGAAG 
AACCTGGTGA CTGACACCAC TTCCGAGCCC TACTTCCTGT CCATCCTGCA GCATTTGCTG 
TACATCAGGG ATGACTTCTA CTTCCGACCT GCCTATTATC AGCTGATTGA GGAGTGCATC 
TCACAAATCG TCTTCCACAA GGGTTACTGT GATCCGAATT TCGAGAACCG GAACTTTAAT 
ATAGACACCT CGCTACTGCT GGACGACATT GTGGAGAAGG CCAAGGCCAA GGAGTCGAAG 
CGGTCGGAGG AGTACGAGAA GAAGATCGAG CAGCTGGAGA GTGCCAAGCA AGAGGCGGAA 
GCGAAGGCGG CTCATCTGGA GGAGAAAGTC AAGTTGATGG AGGCTAATGG TGTGGCGGCT 
CCGTCGCCCA ATAAGCTACC CAAGGTAAAC ATACCCATGC CACCGCCACC ACCAGGAGGA 
GGAGGAGCAC CTCCCCCACC GCCGCCACCT ATGCCGGGAC GAGCAGGTGG TGGACCTCCG 
CCTCCACCAC CACCTCCCAT GCCGGGAAGG GCAGGTGGAC CGCCACCACC TCCTCCACCG 
CCCGGAATGG GAGGCCCACC GCCTCCACCC ATGCCTGGCA TGATGCGCCC AGGAGGGGGA 
CCTCCACCAC CGCCCATGAT GATGGGGCCC ATGGTTCCCG TTCTGCCTCA TGGCTTAAAA 
CCGAAGAAGA AGTGGGACGT CAAGAATCCC ATGAAGCGGG CCAACTGGAA AGCCATTGTC 
CCGGCCAAAA TGTCCGACAA GGCATTCTGG GTCAAGTGCC AGGAGGATAA GCTGGCCCAG 
GATGACTTCC TCGCAGAACT GGCCGTGAAA TTCTCTTCTA AACCTGTAAA GAAAGAACAG 
AAGGATGCGG TGGACAAGCC AACGACGCTG ACAAAGAAAA ATGTCGATCT TCGTGTGCTC 
GACTCGAAGA CTGCTCAGAA CTTGGCTATT ATGTTGGGAG GATCGCTGAA GCATCTGTCC 
TACGAACAGA TCAAGATCTG CTTGCTGCGC TGCGACACCG ACATCCTGTC CTCCAATATC 
CTGCAGCAAC TTATCCAGTA CCTTCCGCCG CCAGAGCACC TCAAGCGTTT GCAGGAGATC 
AAGGCAAAGG GCGAACCGCT ACCGCCGATT GAACAGTTTG CAGCCACAAT AGGGGAAATT 
AAACGCCTTT CGCCGCGACT TCACAACCTG AACTTCAAGC TGACCTACGC GGACATGGTG 
CAGGATATCA AACCCGACAT TGTGGCAGGA ACGGCAGCAT GCGAAGAGAT CCGGAATAGC 
AAAAAGTTCT CAAAGATCTT GGAGCTGATT CTGCTGCTTG GAAATTACAT GAACTCGGGC 
TCCAAAAACG AGGCCGCCTT TGGCTTTGAG ATCAGTTATT TAACCAAACT GTCCAATACG 
AAGGATGCGG ATAATAAGCA GACATTGCTG CACTACCTGG CTGACCTGGT GGAAAAGAAA 
TTCCCAGATG CACTAAACTT CTACGACGAT CTGTCGCATG TTAATAAAGC GTCGCGGGTC 
AACATGGATG CCATCCAAAA GGCCATGCGG CAAATGAATT CGGCGGTTAA GAACCTGGAA 
ACTGATCTCC AGAACAACAA GGTGCCGCAG TGTGATGATG ACAAGTTTAG CGAGGTGATG 
GGCAAGTTTG CCGAGGAGTG CAGACAACAA GTGGACGTGC TGGGCAAAAT GCAGCTGCAA 
ATGGAGAAGC TGTACAAGGA CCTCAGCGAG TACTATGCCT TCGATCCCAG CAAATACACA 
ATGGAGGAGT TCTTTGCGGA CATCAAGACT TTCAAGGATG CCTTCCAAGC GGCCCACAAC 
GACAATGTCC GGGTACGCGA GGAGCTGGAG AAGAAGCGTC GTCTGCAGGA GGCCCGAGAG 
CAGTCTGCTC GAGAGCAACA GGAGCGCCAA CAGCGTAAGA AGGCAGTGGT TGACATGGAT 
GCCCCGCAGA CGCAGGAAGG CGTGATGGAC AGTCTGTTGG AGGCGCTGCA AACGGGCTCA 
GCCTTTGGCC AACGAAATCG ACAGGCCCGG CGACAACGAC CGGCGGGAGC GGAGCGGAGG 
GCACAGCTCA GCCGGAGTCG ATCGCGGACG CGTGTCACCA ACGGACAACT AATGACCCGC 
GAAATGATCC TCAACGAGGT TCTAGGCTCC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dia ( NM_057633.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476981.1 Drosophila melanogaster diaphanous (dia), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15059
Clone ID Related Accession (Same CDS sequence)	NM_001273711.1 , NM_057633.5 , NM_165341.3
Accession Version	NM_057633.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diaphanous, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057633.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGTC ACGAGAAAAC GAAATCCACG GGCGGCGGGC TCCTGGACAG TCTGTTCGGA 
AGACCCTCGA AGTCCAAGGG AGGAACCATC AGCAGTGGCA CCCTGGCCCA TGGCGGACGA 
CCCGTGTCCG CGGACAACTA TGTGGTGCCG GGCGTGGAGG ACTTTGAGCA GTACATCCAG 
CAGCTAAGCG TTGCGGAGCT GGATGCGAAG TTTCTGGAGA TCATCGAGGA CATGAACATT 
CCGAAGGACA AGAGGGAGCC CCTGTTGGCC AAATCGAAGG AGGAGCGACA GAAGATGATT 
ATGTGGCACT TGAAAGGTAA AAACTCACTG GAGCGTAGCG CCAACTCCCG CTTCGAGAAG 
CCCATAGACT ATGTGGAATA CCTGCAGAAT GGGGAGCACA GCACGCACAA GGTGTACCAA 
TGTGTGGAAT CTCTGCGCGT GGCGCTCACC AGCAATCCGA TCTCGTGGAT CAAGGAGTTT 
GGAGTGGCGG GCATCGGGAC GATTGAGAAG CTGCTGGCCC GGTCAAAGAA TAATGCCAGC 
TACGAGAAGA TCGAGTTCGA GGCGATTCGG TGCCTGAAGG CGATCATGAA CAACACATGG 
GGTCTGAACG TGGTGCTCAA TCCGGATCAG CATAGTGTGG TGCTGCTGCT GGCGCAATCC 
CTGGATCCCC GGAAGCCGCA GACGATGTGT GAAGCCCTCA AGCTGCTGGC CTCGTTCTGC 
ATTGTCTATG AGCGGAATGG CTACGAGAAG GTTCTCCGAG CCATAACCAC TATTGCAGCC 
ACATCCTTTA AAGCGAGCGA GCGCTTTCGA CCCATAGTGG ATGCCTTGTT TGCATCGGAT 
CAGCAGGATC CCAAACGGGA CTTGGCATGC CACAGCCTGA TTTTTATTAA TACTCTCACC 
AATACCCCCA CGGATTTGAA CTTCCGCCTG CACTTGCGAT GTGAGATTAT GCGCATGGGT 
CTATACGATC GCCTAGATGA GTTCACCAAA ATCGTGGAGG CCAGCAATAA TGAGAACCTG 
CAGCAGCACT TCAAGATCTT CAACGAGATC CGCGAGGATG ACTTCGAGGA GTTTGTGCAG 
CGCTTCGATA ATGTCACCTT CAACATGGAC GACGCCACCG ATTGCTTCGA TGTGCTGAAG 
AACCTGGTGA CTGACACCAC TTCCGAGCCC TACTTCCTGT CCATCCTGCA GCATTTGCTG 
TACATCAGGG ATGACTTCTA CTTCCGACCT GCCTATTATC AGCTGATTGA GGAGTGCATC 
TCACAAATCG TCTTCCACAA GGGTTACTGT GATCCGAATT TCGAGAACCG GAACTTTAAT 
ATAGACACCT CGCTACTGCT GGACGACATT GTGGAGAAGG CCAAGGCCAA GGAGTCGAAG 
CGGTCGGAGG AGTACGAGAA GAAGATCGAG CAGCTGGAGA GTGCCAAGCA AGAGGCGGAA 
GCGAAGGCGG CTCATCTGGA GGAGAAAGTC AAGTTGATGG AGGCTAATGG TGTGGCGGCT 
CCGTCGCCCA ATAAGCTACC CAAGGTAAAC ATACCCATGC CACCGCCACC ACCAGGAGGA 
GGAGGAGCAC CTCCCCCACC GCCGCCACCT ATGCCGGGAC GAGCAGGTGG TGGACCTCCG 
CCTCCACCAC CACCTCCCAT GCCGGGAAGG GCAGGTGGAC CGCCACCACC TCCTCCACCG 
CCCGGAATGG GAGGCCCACC GCCTCCACCC ATGCCTGGCA TGATGCGCCC AGGAGGGGGA 
CCTCCACCAC CGCCCATGAT GATGGGGCCC ATGGTTCCCG TTCTGCCTCA TGGCTTAAAA 
CCGAAGAAGA AGTGGGACGT CAAGAATCCC ATGAAGCGGG CCAACTGGAA AGCCATTGTC 
CCGGCCAAAA TGTCCGACAA GGCATTCTGG GTCAAGTGCC AGGAGGATAA GCTGGCCCAG 
GATGACTTCC TCGCAGAACT GGCCGTGAAA TTCTCTTCTA AACCTGTAAA GAAAGAACAG 
AAGGATGCGG TGGACAAGCC AACGACGCTG ACAAAGAAAA ATGTCGATCT TCGTGTGCTC 
GACTCGAAGA CTGCTCAGAA CTTGGCTATT ATGTTGGGAG GATCGCTGAA GCATCTGTCC 
TACGAACAGA TCAAGATCTG CTTGCTGCGC TGCGACACCG ACATCCTGTC CTCCAATATC 
CTGCAGCAAC TTATCCAGTA CCTTCCGCCG CCAGAGCACC TCAAGCGTTT GCAGGAGATC 
AAGGCAAAGG GCGAACCGCT ACCGCCGATT GAACAGTTTG CAGCCACAAT AGGGGAAATT 
AAACGCCTTT CGCCGCGACT TCACAACCTG AACTTCAAGC TGACCTACGC GGACATGGTG 
CAGGATATCA AACCCGACAT TGTGGCAGGA ACGGCAGCAT GCGAAGAGAT CCGGAATAGC 
AAAAAGTTCT CAAAGATCTT GGAGCTGATT CTGCTGCTTG GAAATTACAT GAACTCGGGC 
TCCAAAAACG AGGCCGCCTT TGGCTTTGAG ATCAGTTATT TAACCAAACT GTCCAATACG 
AAGGATGCGG ATAATAAGCA GACATTGCTG CACTACCTGG CTGACCTGGT GGAAAAGAAA 
TTCCCAGATG CACTAAACTT CTACGACGAT CTGTCGCATG TTAATAAAGC GTCGCGGGTC 
AACATGGATG CCATCCAAAA GGCCATGCGG CAAATGAATT CGGCGGTTAA GAACCTGGAA 
ACTGATCTCC AGAACAACAA GGTGCCGCAG TGTGATGATG ACAAGTTTAG CGAGGTGATG 
GGCAAGTTTG CCGAGGAGTG CAGACAACAA GTGGACGTGC TGGGCAAAAT GCAGCTGCAA 
ATGGAGAAGC TGTACAAGGA CCTCAGCGAG TACTATGCCT TCGATCCCAG CAAATACACA 
ATGGAGGAGT TCTTTGCGGA CATCAAGACT TTCAAGGATG CCTTCCAAGC GGCCCACAAC 
GACAATGTCC GGGTACGCGA GGAGCTGGAG AAGAAGCGTC GTCTGCAGGA GGCCCGAGAG 
CAGTCTGCTC GAGAGCAACA GGAGCGCCAA CAGCGTAAGA AGGCAGTGGT TGACATGGAT 
GCCCCGCAGA CGCAGGAAGG CGTGATGGAC AGTCTGTTGG AGGCGCTGCA AACGGGCTCA 
GCCTTTGGCC AACGAAATCG ACAGGCCCGG CGACAACGAC CGGCGGGAGC GGAGCGGAGG 
GCACAGCTCA GCCGGAGTCG ATCGCGGACG CGTGTCACCA ACGGACAACT AATGACCCGC 
GAAATGATCC TCAACGAGGT TCTAGGCTCC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476981.1
CDS	522..3797
Translation	MSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQQLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKNSLERSANSRFEKPIDYVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKNNASYEKIEFEAIRCLKAIMNNTWGLNVVLNPDQHSVVLLLAQSLDPRKPQTMCEALKLLASFCIVYERNGYEKVLRAITTIAATSFKASERFRPIVDALFASDQQDPKRDLACHSLIFINTLTNTPTDLNFRLHLRCEIMRMGLYDRLDEFTKIVEASNNENLQQHFKIFNEIREDDFEEFVQRFDNVTFNMDDATDCFDVLKNLVTDTTSEPYFLSILQHLLYIRDDFYFRPAYYQLIEECISQIVFHKGYCDPNFENRNFNIDTSLLLDDIVEKAKAKESKRSEEYEKKIEQLESAKQEAEAKAAHLEEKVKLMEANGVAAPSPNKLPKVNIPMPPPPPGGGGAPPPPPPPMPGRAGGGPPPPPPPPMPGRAGGPPPPPPPPGMGGPPPPPMPGMMRPGGGPPPPPMMMGPMVPVLPHGLKPKKKWDVKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQDDFLAELAVKFSSKPVKKEQKDAVDKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSYEQIKICLLRCDTDILSSNILQQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQFAATIGEIKRLSPRLHNLNFKLTYADMVQDIKPDIVAGTAACEEIRNSKKFSKILELILLLGNYMNSGSKNEAAFGFEISYLTKLSNTKDADNKQTLLHYLADLVEKKFPDALNFYDDLSHVNKASRVNMDAIQKAMRQMNSAVKNLETDLQNNKVPQCDDDKFSEVMGKFAEECRQQVDVLGKMQLQMEKLYKDLSEYYAFDPSKYTMEEFFADIKTFKDAFQAAHNDNVRVREELEKKRRLQEAREQSAREQQERQQRKKAVVDMDAPQTQEGVMDSLLEALQTGSAFGQRNRQARRQRPAGAERRAQLSRSRSRTRVTNGQLMTREMILNEVLGSA

Target ORF information:

RefSeq Version	NM_057633.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diaphanous (dia), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057633.5

ORF Insert Sequence:

ATGTCTCGTC ACGAGAAAAC GAAATCCACG GGCGGCGGGC TCCTGGACAG TCTGTTCGGA 
AGACCCTCGA AGTCCAAGGG AGGAACCATC AGCAGTGGCA CCCTGGCCCA TGGCGGACGA 
CCCGTGTCCG CGGACAACTA TGTGGTGCCG GGCGTGGAGG ACTTTGAGCA GTACATCCAG 
CAGCTAAGCG TTGCGGAGCT GGATGCGAAG TTTCTGGAGA TCATCGAGGA CATGAACATT 
CCGAAGGACA AGAGGGAGCC CCTGTTGGCC AAATCGAAGG AGGAGCGACA GAAGATGATT 
ATGTGGCACT TGAAAGGTAA AAACTCACTG GAGCGTAGCG CCAACTCCCG CTTCGAGAAG 
CCCATAGACT ATGTGGAATA CCTGCAGAAT GGGGAGCACA GCACGCACAA GGTGTACCAA 
TGTGTGGAAT CTCTGCGCGT GGCGCTCACC AGCAATCCGA TCTCGTGGAT CAAGGAGTTT 
GGAGTGGCGG GCATCGGGAC GATTGAGAAG CTGCTGGCCC GGTCAAAGAA TAATGCCAGC 
TACGAGAAGA TCGAGTTCGA GGCGATTCGG TGCCTGAAGG CGATCATGAA CAACACATGG 
GGTCTGAACG TGGTGCTCAA TCCGGATCAG CATAGTGTGG TGCTGCTGCT GGCGCAATCC 
CTGGATCCCC GGAAGCCGCA GACGATGTGT GAAGCCCTCA AGCTGCTGGC CTCGTTCTGC 
ATTGTCTATG AGCGGAATGG CTACGAGAAG GTTCTCCGAG CCATAACCAC TATTGCAGCC 
ACATCCTTTA AAGCGAGCGA GCGCTTTCGA CCCATAGTGG ATGCCTTGTT TGCATCGGAT 
CAGCAGGATC CCAAACGGGA CTTGGCATGC CACAGCCTGA TTTTTATTAA TACTCTCACC 
AATACCCCCA CGGATTTGAA CTTCCGCCTG CACTTGCGAT GTGAGATTAT GCGCATGGGT 
CTATACGATC GCCTAGATGA GTTCACCAAA ATCGTGGAGG CCAGCAATAA TGAGAACCTG 
CAGCAGCACT TCAAGATCTT CAACGAGATC CGCGAGGATG ACTTCGAGGA GTTTGTGCAG 
CGCTTCGATA ATGTCACCTT CAACATGGAC GACGCCACCG ATTGCTTCGA TGTGCTGAAG 
AACCTGGTGA CTGACACCAC TTCCGAGCCC TACTTCCTGT CCATCCTGCA GCATTTGCTG 
TACATCAGGG ATGACTTCTA CTTCCGACCT GCCTATTATC AGCTGATTGA GGAGTGCATC 
TCACAAATCG TCTTCCACAA GGGTTACTGT GATCCGAATT TCGAGAACCG GAACTTTAAT 
ATAGACACCT CGCTACTGCT GGACGACATT GTGGAGAAGG CCAAGGCCAA GGAGTCGAAG 
CGGTCGGAGG AGTACGAGAA GAAGATCGAG CAGCTGGAGA GTGCCAAGCA AGAGGCGGAA 
GCGAAGGCGG CTCATCTGGA GGAGAAAGTC AAGTTGATGG AGGCTAATGG TGTGGCGGCT 
CCGTCGCCCA ATAAGCTACC CAAGGTAAAC ATACCCATGC CACCGCCACC ACCAGGAGGA 
GGAGGAGCAC CTCCCCCACC GCCGCCACCT ATGCCGGGAC GAGCAGGTGG TGGACCTCCG 
CCTCCACCAC CACCTCCCAT GCCGGGAAGG GCAGGTGGAC CGCCACCACC TCCTCCACCG 
CCCGGAATGG GAGGCCCACC GCCTCCACCC ATGCCTGGCA TGATGCGCCC AGGAGGGGGA 
CCTCCACCAC CGCCCATGAT GATGGGGCCC ATGGTTCCCG TTCTGCCTCA TGGCTTAAAA 
CCGAAGAAGA AGTGGGACGT CAAGAATCCC ATGAAGCGGG CCAACTGGAA AGCCATTGTC 
CCGGCCAAAA TGTCCGACAA GGCATTCTGG GTCAAGTGCC AGGAGGATAA GCTGGCCCAG 
GATGACTTCC TCGCAGAACT GGCCGTGAAA TTCTCTTCTA AACCTGTAAA GAAAGAACAG 
AAGGATGCGG TGGACAAGCC AACGACGCTG ACAAAGAAAA ATGTCGATCT TCGTGTGCTC 
GACTCGAAGA CTGCTCAGAA CTTGGCTATT ATGTTGGGAG GATCGCTGAA GCATCTGTCC 
TACGAACAGA TCAAGATCTG CTTGCTGCGC TGCGACACCG ACATCCTGTC CTCCAATATC 
CTGCAGCAAC TTATCCAGTA CCTTCCGCCG CCAGAGCACC TCAAGCGTTT GCAGGAGATC 
AAGGCAAAGG GCGAACCGCT ACCGCCGATT GAACAGTTTG CAGCCACAAT AGGGGAAATT 
AAACGCCTTT CGCCGCGACT TCACAACCTG AACTTCAAGC TGACCTACGC GGACATGGTG 
CAGGATATCA AACCCGACAT TGTGGCAGGA ACGGCAGCAT GCGAAGAGAT CCGGAATAGC 
AAAAAGTTCT CAAAGATCTT GGAGCTGATT CTGCTGCTTG GAAATTACAT GAACTCGGGC 
TCCAAAAACG AGGCCGCCTT TGGCTTTGAG ATCAGTTATT TAACCAAACT GTCCAATACG 
AAGGATGCGG ATAATAAGCA GACATTGCTG CACTACCTGG CTGACCTGGT GGAAAAGAAA 
TTCCCAGATG CACTAAACTT CTACGACGAT CTGTCGCATG TTAATAAAGC GTCGCGGGTC 
AACATGGATG CCATCCAAAA GGCCATGCGG CAAATGAATT CGGCGGTTAA GAACCTGGAA 
ACTGATCTCC AGAACAACAA GGTGCCGCAG TGTGATGATG ACAAGTTTAG CGAGGTGATG 
GGCAAGTTTG CCGAGGAGTG CAGACAACAA GTGGACGTGC TGGGCAAAAT GCAGCTGCAA 
ATGGAGAAGC TGTACAAGGA CCTCAGCGAG TACTATGCCT TCGATCCCAG CAAATACACA 
ATGGAGGAGT TCTTTGCGGA CATCAAGACT TTCAAGGATG CCTTCCAAGC GGCCCACAAC 
GACAATGTCC GGGTACGCGA GGAGCTGGAG AAGAAGCGTC GTCTGCAGGA GGCCCGAGAG 
CAGTCTGCTC GAGAGCAACA GGAGCGCCAA CAGCGTAAGA AGGCAGTGGT TGACATGGAT 
GCCCCGCAGA CGCAGGAAGG CGTGATGGAC AGTCTGTTGG AGGCGCTGCA AACGGGCTCA 
GCCTTTGGCC AACGAAATCG ACAGGCCCGG CGACAACGAC CGGCGGGAGC GGAGCGGAGG 
GCACAGCTCA GCCGGAGTCG ATCGCGGACG CGTGTCACCA ACGGACAACT AATGACCCGC 
GAAATGATCC TCAACGAGGT TCTAGGCTCC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dia ( NM_165341.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724285.1 Drosophila melanogaster diaphanous (dia), transcript variant B, mRNA.

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CloneID	OFa15059
Clone ID Related Accession (Same CDS sequence)	NM_001273711.1 , NM_057633.5 , NM_165341.3
Accession Version	NM_165341.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diaphanous, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165341.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGTC ACGAGAAAAC GAAATCCACG GGCGGCGGGC TCCTGGACAG TCTGTTCGGA 
AGACCCTCGA AGTCCAAGGG AGGAACCATC AGCAGTGGCA CCCTGGCCCA TGGCGGACGA 
CCCGTGTCCG CGGACAACTA TGTGGTGCCG GGCGTGGAGG ACTTTGAGCA GTACATCCAG 
CAGCTAAGCG TTGCGGAGCT GGATGCGAAG TTTCTGGAGA TCATCGAGGA CATGAACATT 
CCGAAGGACA AGAGGGAGCC CCTGTTGGCC AAATCGAAGG AGGAGCGACA GAAGATGATT 
ATGTGGCACT TGAAAGGTAA AAACTCACTG GAGCGTAGCG CCAACTCCCG CTTCGAGAAG 
CCCATAGACT ATGTGGAATA CCTGCAGAAT GGGGAGCACA GCACGCACAA GGTGTACCAA 
TGTGTGGAAT CTCTGCGCGT GGCGCTCACC AGCAATCCGA TCTCGTGGAT CAAGGAGTTT 
GGAGTGGCGG GCATCGGGAC GATTGAGAAG CTGCTGGCCC GGTCAAAGAA TAATGCCAGC 
TACGAGAAGA TCGAGTTCGA GGCGATTCGG TGCCTGAAGG CGATCATGAA CAACACATGG 
GGTCTGAACG TGGTGCTCAA TCCGGATCAG CATAGTGTGG TGCTGCTGCT GGCGCAATCC 
CTGGATCCCC GGAAGCCGCA GACGATGTGT GAAGCCCTCA AGCTGCTGGC CTCGTTCTGC 
ATTGTCTATG AGCGGAATGG CTACGAGAAG GTTCTCCGAG CCATAACCAC TATTGCAGCC 
ACATCCTTTA AAGCGAGCGA GCGCTTTCGA CCCATAGTGG ATGCCTTGTT TGCATCGGAT 
CAGCAGGATC CCAAACGGGA CTTGGCATGC CACAGCCTGA TTTTTATTAA TACTCTCACC 
AATACCCCCA CGGATTTGAA CTTCCGCCTG CACTTGCGAT GTGAGATTAT GCGCATGGGT 
CTATACGATC GCCTAGATGA GTTCACCAAA ATCGTGGAGG CCAGCAATAA TGAGAACCTG 
CAGCAGCACT TCAAGATCTT CAACGAGATC CGCGAGGATG ACTTCGAGGA GTTTGTGCAG 
CGCTTCGATA ATGTCACCTT CAACATGGAC GACGCCACCG ATTGCTTCGA TGTGCTGAAG 
AACCTGGTGA CTGACACCAC TTCCGAGCCC TACTTCCTGT CCATCCTGCA GCATTTGCTG 
TACATCAGGG ATGACTTCTA CTTCCGACCT GCCTATTATC AGCTGATTGA GGAGTGCATC 
TCACAAATCG TCTTCCACAA GGGTTACTGT GATCCGAATT TCGAGAACCG GAACTTTAAT 
ATAGACACCT CGCTACTGCT GGACGACATT GTGGAGAAGG CCAAGGCCAA GGAGTCGAAG 
CGGTCGGAGG AGTACGAGAA GAAGATCGAG CAGCTGGAGA GTGCCAAGCA AGAGGCGGAA 
GCGAAGGCGG CTCATCTGGA GGAGAAAGTC AAGTTGATGG AGGCTAATGG TGTGGCGGCT 
CCGTCGCCCA ATAAGCTACC CAAGGTAAAC ATACCCATGC CACCGCCACC ACCAGGAGGA 
GGAGGAGCAC CTCCCCCACC GCCGCCACCT ATGCCGGGAC GAGCAGGTGG TGGACCTCCG 
CCTCCACCAC CACCTCCCAT GCCGGGAAGG GCAGGTGGAC CGCCACCACC TCCTCCACCG 
CCCGGAATGG GAGGCCCACC GCCTCCACCC ATGCCTGGCA TGATGCGCCC AGGAGGGGGA 
CCTCCACCAC CGCCCATGAT GATGGGGCCC ATGGTTCCCG TTCTGCCTCA TGGCTTAAAA 
CCGAAGAAGA AGTGGGACGT CAAGAATCCC ATGAAGCGGG CCAACTGGAA AGCCATTGTC 
CCGGCCAAAA TGTCCGACAA GGCATTCTGG GTCAAGTGCC AGGAGGATAA GCTGGCCCAG 
GATGACTTCC TCGCAGAACT GGCCGTGAAA TTCTCTTCTA AACCTGTAAA GAAAGAACAG 
AAGGATGCGG TGGACAAGCC AACGACGCTG ACAAAGAAAA ATGTCGATCT TCGTGTGCTC 
GACTCGAAGA CTGCTCAGAA CTTGGCTATT ATGTTGGGAG GATCGCTGAA GCATCTGTCC 
TACGAACAGA TCAAGATCTG CTTGCTGCGC TGCGACACCG ACATCCTGTC CTCCAATATC 
CTGCAGCAAC TTATCCAGTA CCTTCCGCCG CCAGAGCACC TCAAGCGTTT GCAGGAGATC 
AAGGCAAAGG GCGAACCGCT ACCGCCGATT GAACAGTTTG CAGCCACAAT AGGGGAAATT 
AAACGCCTTT CGCCGCGACT TCACAACCTG AACTTCAAGC TGACCTACGC GGACATGGTG 
CAGGATATCA AACCCGACAT TGTGGCAGGA ACGGCAGCAT GCGAAGAGAT CCGGAATAGC 
AAAAAGTTCT CAAAGATCTT GGAGCTGATT CTGCTGCTTG GAAATTACAT GAACTCGGGC 
TCCAAAAACG AGGCCGCCTT TGGCTTTGAG ATCAGTTATT TAACCAAACT GTCCAATACG 
AAGGATGCGG ATAATAAGCA GACATTGCTG CACTACCTGG CTGACCTGGT GGAAAAGAAA 
TTCCCAGATG CACTAAACTT CTACGACGAT CTGTCGCATG TTAATAAAGC GTCGCGGGTC 
AACATGGATG CCATCCAAAA GGCCATGCGG CAAATGAATT CGGCGGTTAA GAACCTGGAA 
ACTGATCTCC AGAACAACAA GGTGCCGCAG TGTGATGATG ACAAGTTTAG CGAGGTGATG 
GGCAAGTTTG CCGAGGAGTG CAGACAACAA GTGGACGTGC TGGGCAAAAT GCAGCTGCAA 
ATGGAGAAGC TGTACAAGGA CCTCAGCGAG TACTATGCCT TCGATCCCAG CAAATACACA 
ATGGAGGAGT TCTTTGCGGA CATCAAGACT TTCAAGGATG CCTTCCAAGC GGCCCACAAC 
GACAATGTCC GGGTACGCGA GGAGCTGGAG AAGAAGCGTC GTCTGCAGGA GGCCCGAGAG 
CAGTCTGCTC GAGAGCAACA GGAGCGCCAA CAGCGTAAGA AGGCAGTGGT TGACATGGAT 
GCCCCGCAGA CGCAGGAAGG CGTGATGGAC AGTCTGTTGG AGGCGCTGCA AACGGGCTCA 
GCCTTTGGCC AACGAAATCG ACAGGCCCGG CGACAACGAC CGGCGGGAGC GGAGCGGAGG 
GCACAGCTCA GCCGGAGTCG ATCGCGGACG CGTGTCACCA ACGGACAACT AATGACCCGC 
GAAATGATCC TCAACGAGGT TCTAGGCTCC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724285.1
CDS	244..3519
Translation	MSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQQLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKNSLERSANSRFEKPIDYVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKNNASYEKIEFEAIRCLKAIMNNTWGLNVVLNPDQHSVVLLLAQSLDPRKPQTMCEALKLLASFCIVYERNGYEKVLRAITTIAATSFKASERFRPIVDALFASDQQDPKRDLACHSLIFINTLTNTPTDLNFRLHLRCEIMRMGLYDRLDEFTKIVEASNNENLQQHFKIFNEIREDDFEEFVQRFDNVTFNMDDATDCFDVLKNLVTDTTSEPYFLSILQHLLYIRDDFYFRPAYYQLIEECISQIVFHKGYCDPNFENRNFNIDTSLLLDDIVEKAKAKESKRSEEYEKKIEQLESAKQEAEAKAAHLEEKVKLMEANGVAAPSPNKLPKVNIPMPPPPPGGGGAPPPPPPPMPGRAGGGPPPPPPPPMPGRAGGPPPPPPPPGMGGPPPPPMPGMMRPGGGPPPPPMMMGPMVPVLPHGLKPKKKWDVKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQDDFLAELAVKFSSKPVKKEQKDAVDKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSYEQIKICLLRCDTDILSSNILQQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQFAATIGEIKRLSPRLHNLNFKLTYADMVQDIKPDIVAGTAACEEIRNSKKFSKILELILLLGNYMNSGSKNEAAFGFEISYLTKLSNTKDADNKQTLLHYLADLVEKKFPDALNFYDDLSHVNKASRVNMDAIQKAMRQMNSAVKNLETDLQNNKVPQCDDDKFSEVMGKFAEECRQQVDVLGKMQLQMEKLYKDLSEYYAFDPSKYTMEEFFADIKTFKDAFQAAHNDNVRVREELEKKRRLQEAREQSAREQQERQQRKKAVVDMDAPQTQEGVMDSLLEALQTGSAFGQRNRQARRQRPAGAERRAQLSRSRSRTRVTNGQLMTREMILNEVLGSA

Target ORF information:

RefSeq Version	NM_165341.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diaphanous (dia), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165341.3

ORF Insert Sequence:

ATGTCTCGTC ACGAGAAAAC GAAATCCACG GGCGGCGGGC TCCTGGACAG TCTGTTCGGA 
AGACCCTCGA AGTCCAAGGG AGGAACCATC AGCAGTGGCA CCCTGGCCCA TGGCGGACGA 
CCCGTGTCCG CGGACAACTA TGTGGTGCCG GGCGTGGAGG ACTTTGAGCA GTACATCCAG 
CAGCTAAGCG TTGCGGAGCT GGATGCGAAG TTTCTGGAGA TCATCGAGGA CATGAACATT 
CCGAAGGACA AGAGGGAGCC CCTGTTGGCC AAATCGAAGG AGGAGCGACA GAAGATGATT 
ATGTGGCACT TGAAAGGTAA AAACTCACTG GAGCGTAGCG CCAACTCCCG CTTCGAGAAG 
CCCATAGACT ATGTGGAATA CCTGCAGAAT GGGGAGCACA GCACGCACAA GGTGTACCAA 
TGTGTGGAAT CTCTGCGCGT GGCGCTCACC AGCAATCCGA TCTCGTGGAT CAAGGAGTTT 
GGAGTGGCGG GCATCGGGAC GATTGAGAAG CTGCTGGCCC GGTCAAAGAA TAATGCCAGC 
TACGAGAAGA TCGAGTTCGA GGCGATTCGG TGCCTGAAGG CGATCATGAA CAACACATGG 
GGTCTGAACG TGGTGCTCAA TCCGGATCAG CATAGTGTGG TGCTGCTGCT GGCGCAATCC 
CTGGATCCCC GGAAGCCGCA GACGATGTGT GAAGCCCTCA AGCTGCTGGC CTCGTTCTGC 
ATTGTCTATG AGCGGAATGG CTACGAGAAG GTTCTCCGAG CCATAACCAC TATTGCAGCC 
ACATCCTTTA AAGCGAGCGA GCGCTTTCGA CCCATAGTGG ATGCCTTGTT TGCATCGGAT 
CAGCAGGATC CCAAACGGGA CTTGGCATGC CACAGCCTGA TTTTTATTAA TACTCTCACC 
AATACCCCCA CGGATTTGAA CTTCCGCCTG CACTTGCGAT GTGAGATTAT GCGCATGGGT 
CTATACGATC GCCTAGATGA GTTCACCAAA ATCGTGGAGG CCAGCAATAA TGAGAACCTG 
CAGCAGCACT TCAAGATCTT CAACGAGATC CGCGAGGATG ACTTCGAGGA GTTTGTGCAG 
CGCTTCGATA ATGTCACCTT CAACATGGAC GACGCCACCG ATTGCTTCGA TGTGCTGAAG 
AACCTGGTGA CTGACACCAC TTCCGAGCCC TACTTCCTGT CCATCCTGCA GCATTTGCTG 
TACATCAGGG ATGACTTCTA CTTCCGACCT GCCTATTATC AGCTGATTGA GGAGTGCATC 
TCACAAATCG TCTTCCACAA GGGTTACTGT GATCCGAATT TCGAGAACCG GAACTTTAAT 
ATAGACACCT CGCTACTGCT GGACGACATT GTGGAGAAGG CCAAGGCCAA GGAGTCGAAG 
CGGTCGGAGG AGTACGAGAA GAAGATCGAG CAGCTGGAGA GTGCCAAGCA AGAGGCGGAA 
GCGAAGGCGG CTCATCTGGA GGAGAAAGTC AAGTTGATGG AGGCTAATGG TGTGGCGGCT 
CCGTCGCCCA ATAAGCTACC CAAGGTAAAC ATACCCATGC CACCGCCACC ACCAGGAGGA 
GGAGGAGCAC CTCCCCCACC GCCGCCACCT ATGCCGGGAC GAGCAGGTGG TGGACCTCCG 
CCTCCACCAC CACCTCCCAT GCCGGGAAGG GCAGGTGGAC CGCCACCACC TCCTCCACCG 
CCCGGAATGG GAGGCCCACC GCCTCCACCC ATGCCTGGCA TGATGCGCCC AGGAGGGGGA 
CCTCCACCAC CGCCCATGAT GATGGGGCCC ATGGTTCCCG TTCTGCCTCA TGGCTTAAAA 
CCGAAGAAGA AGTGGGACGT CAAGAATCCC ATGAAGCGGG CCAACTGGAA AGCCATTGTC 
CCGGCCAAAA TGTCCGACAA GGCATTCTGG GTCAAGTGCC AGGAGGATAA GCTGGCCCAG 
GATGACTTCC TCGCAGAACT GGCCGTGAAA TTCTCTTCTA AACCTGTAAA GAAAGAACAG 
AAGGATGCGG TGGACAAGCC AACGACGCTG ACAAAGAAAA ATGTCGATCT TCGTGTGCTC 
GACTCGAAGA CTGCTCAGAA CTTGGCTATT ATGTTGGGAG GATCGCTGAA GCATCTGTCC 
TACGAACAGA TCAAGATCTG CTTGCTGCGC TGCGACACCG ACATCCTGTC CTCCAATATC 
CTGCAGCAAC TTATCCAGTA CCTTCCGCCG CCAGAGCACC TCAAGCGTTT GCAGGAGATC 
AAGGCAAAGG GCGAACCGCT ACCGCCGATT GAACAGTTTG CAGCCACAAT AGGGGAAATT 
AAACGCCTTT CGCCGCGACT TCACAACCTG AACTTCAAGC TGACCTACGC GGACATGGTG 
CAGGATATCA AACCCGACAT TGTGGCAGGA ACGGCAGCAT GCGAAGAGAT CCGGAATAGC 
AAAAAGTTCT CAAAGATCTT GGAGCTGATT CTGCTGCTTG GAAATTACAT GAACTCGGGC 
TCCAAAAACG AGGCCGCCTT TGGCTTTGAG ATCAGTTATT TAACCAAACT GTCCAATACG 
AAGGATGCGG ATAATAAGCA GACATTGCTG CACTACCTGG CTGACCTGGT GGAAAAGAAA 
TTCCCAGATG CACTAAACTT CTACGACGAT CTGTCGCATG TTAATAAAGC GTCGCGGGTC 
AACATGGATG CCATCCAAAA GGCCATGCGG CAAATGAATT CGGCGGTTAA GAACCTGGAA 
ACTGATCTCC AGAACAACAA GGTGCCGCAG TGTGATGATG ACAAGTTTAG CGAGGTGATG 
GGCAAGTTTG CCGAGGAGTG CAGACAACAA GTGGACGTGC TGGGCAAAAT GCAGCTGCAA 
ATGGAGAAGC TGTACAAGGA CCTCAGCGAG TACTATGCCT TCGATCCCAG CAAATACACA 
ATGGAGGAGT TCTTTGCGGA CATCAAGACT TTCAAGGATG CCTTCCAAGC GGCCCACAAC 
GACAATGTCC GGGTACGCGA GGAGCTGGAG AAGAAGCGTC GTCTGCAGGA GGCCCGAGAG 
CAGTCTGCTC GAGAGCAACA GGAGCGCCAA CAGCGTAAGA AGGCAGTGGT TGACATGGAT 
GCCCCGCAGA CGCAGGAAGG CGTGATGGAC AGTCTGTTGG AGGCGCTGCA AACGGGCTCA 
GCCTTTGGCC AACGAAATCG ACAGGCCCGG CGACAACGAC CGGCGGGAGC GGAGCGGAGG 
GCACAGCTCA GCCGGAGTCG ATCGCGGACG CGTGTCACCA ACGGACAACT AATGACCCGC 
GAAATGATCC TCAACGAGGT TCTAGGCTCC GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dia ( NM_001259184.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246113.1 Drosophila melanogaster diaphanous (dia), transcript variant D, mRNA.

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CloneID	OFa16949
Clone ID Related Accession (Same CDS sequence)	NM_001259184.1
Accession Version	NM_001259184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diaphanous, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCCTCA AAAAGCTGGG AATGTCTCGT CACGAGAAAA CGAAATCCAC GGGCGGCGGG 
CTCCTGGACA GTCTGTTCGG AAGACCCTCG AAGTCCAAGG GAGGAACCAT CAGCAGTGGC 
ACCCTGGCCC ATGGCGGACG ACCCGTGTCC GCGGACAACT ATGTGGTGCC GGGCGTGGAG 
GACTTTGAGC AGTACATCCA GCAGCTAAGC GTTGCGGAGC TGGATGCGAA GTTTCTGGAG 
ATCATCGAGG ACATGAACAT TCCGAAGGAC AAGAGGGAGC CCCTGTTGGC CAAATCGAAG 
GAGGAGCGAC AGAAGATGAT TATGTGGCAC TTGAAAGGTA AAAACTCACT GGAGCGTAGC 
GCCAACTCCC GCTTCGAGAA GCCCATAGAC TATGTGGAAT ACCTGCAGAA TGGGGAGCAC 
AGCACGCACA AGGTGTACCA ATGTGTGGAA TCTCTGCGCG TGGCGCTCAC CAGCAATCCG 
ATCTCGTGGA TCAAGGAGTT TGGAGTGGCG GGCATCGGGA CGATTGAGAA GCTGCTGGCC 
CGGTCAAAGA ATAATGCCAG CTACGAGAAG ATCGAGTTCG AGGCGATTCG GTGCCTGAAG 
GCGATCATGA ACAACACATG GGGTCTGAAC GTGGTGCTCA ATCCGGATCA GCATAGTGTG 
GTGCTGCTGC TGGCGCAATC CCTGGATCCC CGGAAGCCGC AGACGATGTG TGAAGCCCTC 
AAGCTGCTGG CCTCGTTCTG CATTGTCTAT GAGCGGAATG GCTACGAGAA GGTTCTCCGA 
GCCATAACCA CTATTGCAGC CACATCCTTT AAAGCGAGCG AGCGCTTTCG ACCCATAGTG 
GATGCCTTGT TTGCATCGGA TCAGCAGGAT CCCAAACGGG ACTTGGCATG CCACAGCCTG 
ATTTTTATTA ATACTCTCAC CAATACCCCC ACGGATTTGA ACTTCCGCCT GCACTTGCGA 
TGTGAGATTA TGCGCATGGG TCTATACGAT CGCCTAGATG AGTTCACCAA AATCGTGGAG 
GCCAGCAATA ATGAGAACCT GCAGCAGCAC TTCAAGATCT TCAACGAGAT CCGCGAGGAT 
GACTTCGAGG AGTTTGTGCA GCGCTTCGAT AATGTCACCT TCAACATGGA CGACGCCACC 
GATTGCTTCG ATGTGCTGAA GAACCTGGTG ACTGACACCA CTTCCGAGCC CTACTTCCTG 
TCCATCCTGC AGCATTTGCT GTACATCAGG GATGACTTCT ACTTCCGACC TGCCTATTAT 
CAGCTGATTG AGGAGTGCAT CTCACAAATC GTCTTCCACA AGGGTTACTG TGATCCGAAT 
TTCGAGAACC GGAACTTTAA TATAGACACC TCGCTACTGC TGGACGACAT TGTGGAGAAG 
GCCAAGGCCA AGGAGTCGAA GCGGTCGGAG GAGTACGAGA AGAAGATCGA GCAGCTGGAG 
AGTGCCAAGC AAGAGGCGGA AGCGAAGGCG GCTCATCTGG AGGAGAAAGT CAAGTTGATG 
GAGGCTAATG GTGTGGCGGC TCCGTCGCCC AATAAGCTAC CCAAGGTAAA CATACCCATG 
CCACCGCCAC CACCAGGAGG AGGAGGAGCA CCTCCCCCAC CGCCGCCACC TATGCCGGGA 
CGAGCAGGTG GTGGACCTCC GCCTCCACCA CCACCTCCCA TGCCGGGAAG GGCAGGTGGA 
CCGCCACCAC CTCCTCCACC GCCCGGAATG GGAGGCCCAC CGCCTCCACC CATGCCTGGC 
ATGATGCGCC CAGGAGGGGG ACCTCCACCA CCGCCCATGA TGATGGGGCC CATGGTTCCC 
GTTCTGCCTC ATGGCTTAAA ACCGAAGAAG AAGTGGGACG TCAAGAATCC CATGAAGCGG 
GCCAACTGGA AAGCCATTGT CCCGGCCAAA ATGTCCGACA AGGCATTCTG GGTCAAGTGC 
CAGGAGGATA AGCTGGCCCA GGATGACTTC CTCGCAGAAC TGGCCGTGAA ATTCTCTTCT 
AAACCTGTAA AGAAAGAACA GAAGGATGCG GTGGACAAGC CAACGACGCT GACAAAGAAA 
AATGTCGATC TTCGTGTGCT CGACTCGAAG ACTGCTCAGA ACTTGGCTAT TATGTTGGGA 
GGATCGCTGA AGCATCTGTC CTACGAACAG ATCAAGATCT GCTTGCTGCG CTGCGACACC 
GACATCCTGT CCTCCAATAT CCTGCAGCAA CTTATCCAGT ACCTTCCGCC GCCAGAGCAC 
CTCAAGCGTT TGCAGGAGAT CAAGGCAAAG GGCGAACCGC TACCGCCGAT TGAACAGTTT 
GCAGCCACAA TAGGGGAAAT TAAACGCCTT TCGCCGCGAC TTCACAACCT GAACTTCAAG 
CTGACCTACG CGGACATGGT GCAGGATATC AAACCCGACA TTGTGGCAGG AACGGCAGCA 
TGCGAAGAGA TCCGGAATAG CAAAAAGTTC TCAAAGATCT TGGAGCTGAT TCTGCTGCTT 
GGAAATTACA TGAACTCGGG CTCCAAAAAC GAGGCCGCCT TTGGCTTTGA GATCAGTTAT 
TTAACCAAAC TGTCCAATAC GAAGGATGCG GATAATAAGC AGACATTGCT GCACTACCTG 
GCTGACCTGG TGGAAAAGAA ATTCCCAGAT GCACTAAACT TCTACGACGA TCTGTCGCAT 
GTTAATAAAG CGTCGCGGGT CAACATGGAT GCCATCCAAA AGGCCATGCG GCAAATGAAT 
TCGGCGGTTA AGAACCTGGA AACTGATCTC CAGAACAACA AGGTGCCGCA GTGTGATGAT 
GACAAGTTTA GCGAGGTGAT GGGCAAGTTT GCCGAGGAGT GCAGACAACA AGTGGACGTG 
CTGGGCAAAA TGCAGCTGCA AATGGAGAAG CTGTACAAGG ACCTCAGCGA GTACTATGCC 
TTCGATCCCA GCAAATACAC AATGGAGGAG TTCTTTGCGG ACATCAAGAC TTTCAAGGAT 
GCCTTCCAAG CGGCCCACAA CGACAATGTC CGGGTACGCG AGGAGCTGGA GAAGAAGCGT 
CGTCTGCAGG AGGCCCGAGA GCAGTCTGCT CGAGAGCAAC AGGAGCGCCA ACAGCGTAAG 
AAGGCAGTGG TTGACATGGA TGCCCCGCAG ACGCAGGAAG GCGTGATGGA CAGTCTGTTG 
GAGGCGCTGC AAACGGGCTC AGCCTTTGGC CAACGAAATC GACAGGCCCG GCGACAACGA 
CCGGCGGGAG CGGAGCGGAG GGCACAGCTC AGCCGGAGTC GATCGCGGAC GCGTGTCACC 
AACGGACAAC TAATGACCCG CGAAATGATC CTCAACGAGG TTCTAGGCTC CGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246113.1
CDS	139..3435
Translation	MRLKKLGMSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQQLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKNSLERSANSRFEKPIDYVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKNNASYEKIEFEAIRCLKAIMNNTWGLNVVLNPDQHSVVLLLAQSLDPRKPQTMCEALKLLASFCIVYERNGYEKVLRAITTIAATSFKASERFRPIVDALFASDQQDPKRDLACHSLIFINTLTNTPTDLNFRLHLRCEIMRMGLYDRLDEFTKIVEASNNENLQQHFKIFNEIREDDFEEFVQRFDNVTFNMDDATDCFDVLKNLVTDTTSEPYFLSILQHLLYIRDDFYFRPAYYQLIEECISQIVFHKGYCDPNFENRNFNIDTSLLLDDIVEKAKAKESKRSEEYEKKIEQLESAKQEAEAKAAHLEEKVKLMEANGVAAPSPNKLPKVNIPMPPPPPGGGGAPPPPPPPMPGRAGGGPPPPPPPPMPGRAGGPPPPPPPPGMGGPPPPPMPGMMRPGGGPPPPPMMMGPMVPVLPHGLKPKKKWDVKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQDDFLAELAVKFSSKPVKKEQKDAVDKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSYEQIKICLLRCDTDILSSNILQQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQFAATIGEIKRLSPRLHNLNFKLTYADMVQDIKPDIVAGTAACEEIRNSKKFSKILELILLLGNYMNSGSKNEAAFGFEISYLTKLSNTKDADNKQTLLHYLADLVEKKFPDALNFYDDLSHVNKASRVNMDAIQKAMRQMNSAVKNLETDLQNNKVPQCDDDKFSEVMGKFAEECRQQVDVLGKMQLQMEKLYKDLSEYYAFDPSKYTMEEFFADIKTFKDAFQAAHNDNVRVREELEKKRRLQEAREQSAREQQERQQRKKAVVDMDAPQTQEGVMDSLLEALQTGSAFGQRNRQARRQRPAGAERRAQLSRSRSRTRVTNGQLMTREMILNEVLGSA

Target ORF information:

RefSeq Version	NM_001259184.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diaphanous (dia), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259184.1

ORF Insert Sequence:

ATGCGCCTCA AAAAGCTGGG AATGTCTCGT CACGAGAAAA CGAAATCCAC GGGCGGCGGG 
CTCCTGGACA GTCTGTTCGG AAGACCCTCG AAGTCCAAGG GAGGAACCAT CAGCAGTGGC 
ACCCTGGCCC ATGGCGGACG ACCCGTGTCC GCGGACAACT ATGTGGTGCC GGGCGTGGAG 
GACTTTGAGC AGTACATCCA GCAGCTAAGC GTTGCGGAGC TGGATGCGAA GTTTCTGGAG 
ATCATCGAGG ACATGAACAT TCCGAAGGAC AAGAGGGAGC CCCTGTTGGC CAAATCGAAG 
GAGGAGCGAC AGAAGATGAT TATGTGGCAC TTGAAAGGTA AAAACTCACT GGAGCGTAGC 
GCCAACTCCC GCTTCGAGAA GCCCATAGAC TATGTGGAAT ACCTGCAGAA TGGGGAGCAC 
AGCACGCACA AGGTGTACCA ATGTGTGGAA TCTCTGCGCG TGGCGCTCAC CAGCAATCCG 
ATCTCGTGGA TCAAGGAGTT TGGAGTGGCG GGCATCGGGA CGATTGAGAA GCTGCTGGCC 
CGGTCAAAGA ATAATGCCAG CTACGAGAAG ATCGAGTTCG AGGCGATTCG GTGCCTGAAG 
GCGATCATGA ACAACACATG GGGTCTGAAC GTGGTGCTCA ATCCGGATCA GCATAGTGTG 
GTGCTGCTGC TGGCGCAATC CCTGGATCCC CGGAAGCCGC AGACGATGTG TGAAGCCCTC 
AAGCTGCTGG CCTCGTTCTG CATTGTCTAT GAGCGGAATG GCTACGAGAA GGTTCTCCGA 
GCCATAACCA CTATTGCAGC CACATCCTTT AAAGCGAGCG AGCGCTTTCG ACCCATAGTG 
GATGCCTTGT TTGCATCGGA TCAGCAGGAT CCCAAACGGG ACTTGGCATG CCACAGCCTG 
ATTTTTATTA ATACTCTCAC CAATACCCCC ACGGATTTGA ACTTCCGCCT GCACTTGCGA 
TGTGAGATTA TGCGCATGGG TCTATACGAT CGCCTAGATG AGTTCACCAA AATCGTGGAG 
GCCAGCAATA ATGAGAACCT GCAGCAGCAC TTCAAGATCT TCAACGAGAT CCGCGAGGAT 
GACTTCGAGG AGTTTGTGCA GCGCTTCGAT AATGTCACCT TCAACATGGA CGACGCCACC 
GATTGCTTCG ATGTGCTGAA GAACCTGGTG ACTGACACCA CTTCCGAGCC CTACTTCCTG 
TCCATCCTGC AGCATTTGCT GTACATCAGG GATGACTTCT ACTTCCGACC TGCCTATTAT 
CAGCTGATTG AGGAGTGCAT CTCACAAATC GTCTTCCACA AGGGTTACTG TGATCCGAAT 
TTCGAGAACC GGAACTTTAA TATAGACACC TCGCTACTGC TGGACGACAT TGTGGAGAAG 
GCCAAGGCCA AGGAGTCGAA GCGGTCGGAG GAGTACGAGA AGAAGATCGA GCAGCTGGAG 
AGTGCCAAGC AAGAGGCGGA AGCGAAGGCG GCTCATCTGG AGGAGAAAGT CAAGTTGATG 
GAGGCTAATG GTGTGGCGGC TCCGTCGCCC AATAAGCTAC CCAAGGTAAA CATACCCATG 
CCACCGCCAC CACCAGGAGG AGGAGGAGCA CCTCCCCCAC CGCCGCCACC TATGCCGGGA 
CGAGCAGGTG GTGGACCTCC GCCTCCACCA CCACCTCCCA TGCCGGGAAG GGCAGGTGGA 
CCGCCACCAC CTCCTCCACC GCCCGGAATG GGAGGCCCAC CGCCTCCACC CATGCCTGGC 
ATGATGCGCC CAGGAGGGGG ACCTCCACCA CCGCCCATGA TGATGGGGCC CATGGTTCCC 
GTTCTGCCTC ATGGCTTAAA ACCGAAGAAG AAGTGGGACG TCAAGAATCC CATGAAGCGG 
GCCAACTGGA AAGCCATTGT CCCGGCCAAA ATGTCCGACA AGGCATTCTG GGTCAAGTGC 
CAGGAGGATA AGCTGGCCCA GGATGACTTC CTCGCAGAAC TGGCCGTGAA ATTCTCTTCT 
AAACCTGTAA AGAAAGAACA GAAGGATGCG GTGGACAAGC CAACGACGCT GACAAAGAAA 
AATGTCGATC TTCGTGTGCT CGACTCGAAG ACTGCTCAGA ACTTGGCTAT TATGTTGGGA 
GGATCGCTGA AGCATCTGTC CTACGAACAG ATCAAGATCT GCTTGCTGCG CTGCGACACC 
GACATCCTGT CCTCCAATAT CCTGCAGCAA CTTATCCAGT ACCTTCCGCC GCCAGAGCAC 
CTCAAGCGTT TGCAGGAGAT CAAGGCAAAG GGCGAACCGC TACCGCCGAT TGAACAGTTT 
GCAGCCACAA TAGGGGAAAT TAAACGCCTT TCGCCGCGAC TTCACAACCT GAACTTCAAG 
CTGACCTACG CGGACATGGT GCAGGATATC AAACCCGACA TTGTGGCAGG AACGGCAGCA 
TGCGAAGAGA TCCGGAATAG CAAAAAGTTC TCAAAGATCT TGGAGCTGAT TCTGCTGCTT 
GGAAATTACA TGAACTCGGG CTCCAAAAAC GAGGCCGCCT TTGGCTTTGA GATCAGTTAT 
TTAACCAAAC TGTCCAATAC GAAGGATGCG GATAATAAGC AGACATTGCT GCACTACCTG 
GCTGACCTGG TGGAAAAGAA ATTCCCAGAT GCACTAAACT TCTACGACGA TCTGTCGCAT 
GTTAATAAAG CGTCGCGGGT CAACATGGAT GCCATCCAAA AGGCCATGCG GCAAATGAAT 
TCGGCGGTTA AGAACCTGGA AACTGATCTC CAGAACAACA AGGTGCCGCA GTGTGATGAT 
GACAAGTTTA GCGAGGTGAT GGGCAAGTTT GCCGAGGAGT GCAGACAACA AGTGGACGTG 
CTGGGCAAAA TGCAGCTGCA AATGGAGAAG CTGTACAAGG ACCTCAGCGA GTACTATGCC 
TTCGATCCCA GCAAATACAC AATGGAGGAG TTCTTTGCGG ACATCAAGAC TTTCAAGGAT 
GCCTTCCAAG CGGCCCACAA CGACAATGTC CGGGTACGCG AGGAGCTGGA GAAGAAGCGT 
CGTCTGCAGG AGGCCCGAGA GCAGTCTGCT CGAGAGCAAC AGGAGCGCCA ACAGCGTAAG 
AAGGCAGTGG TTGACATGGA TGCCCCGCAG ACGCAGGAAG GCGTGATGGA CAGTCTGTTG 
GAGGCGCTGC AAACGGGCTC AGCCTTTGGC CAACGAAATC GACAGGCCCG GCGACAACGA 
CCGGCGGGAG CGGAGCGGAG GGCACAGCTC AGCCGGAGTC GATCGCGGAC GCGTGTCACC 
AACGGACAAC TAATGACCCG CGAAATGATC CTCAACGAGG TTCTAGGCTC CGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dia cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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