vig cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vig
Entrez Gene ID	34885
Full Name	vasa intronic gene
Synonyms	BG:DS00929.13,CG4170,Dmel\CG4170,EP(2)0812,VIG,Vig
General protein information	Preferred Names vasa intronic gene Names CG4170 gene product from transcript CG4170-RD CG4170-PA CG4170-PB CG4170-PC CG4170-PD vig-PA vig-PB vig-PC vig-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35B10-35C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165102.2	NP_723898.1	vasa intronic gene, isoform D
NM_165101.2	NP_723897.1	vasa intronic gene, isoform C
NM_078848.3	NP_523572.1	vasa intronic gene, isoform A
NM_165100.2	NP_723896.1	vasa intronic gene, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	vig	NP_523572.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013112	XP_003435815.1
Drosophila melanogaster (fruit fly)	fdy	NP_001163863.3
Drosophila melanogaster (fruit fly)	vig2	NP_733083.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fragile X-related protein and VIG associate with the RNA interference machinery
Title: Fragile X-related protein and VIG associate with the RNA interference machinery.

The following vig gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vig cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15892	NM_165102.2 Latest version!	Drosophila melanogaster vasa intronic gene (vig), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15892	NM_165101.2 Latest version!	Drosophila melanogaster vasa intronic gene (vig), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15892	NM_165100.2 Latest version!	Drosophila melanogaster vasa intronic gene (vig), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15892	NM_078848.3 Latest version!	Drosophila melanogaster vasa intronic gene (vig), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vig ( NM_165102.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723898.1 Drosophila melanogaster vasa intronic gene (vig), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15892
Clone ID Related Accession (Same CDS sequence)	NM_165102.2 , NM_165101.2 , NM_078848.3 , NM_165100.2
Accession Version	NM_165102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa intronic gene, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165102.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723898.1
CDS	234..1706
Translation	MDSAGKNRYELLFMDDDVSDPLDNLVAPTAAAAAAAAGKKKQPSAAAAAATKTTANKVANSNNKANAGSNIGGPNAKKPNQAEKENKPNNALNKTDGKKFTPSADNKQQQFNNNASSNYKQQGAPRQGGGANRTREFGSGQGQGQGQGGQQQRSVNFRQQNGNAETREQRNNRRNVRENVGAPDGQQSRPYRGPGGGPGAGGDRPQRQNRNYDGQNRKREFDRQSGSDRTGVKSIDKRDGAGSHNWGSVKEAIDDVNKNESETNVTNAEGGAKADESGTEPQNEQATAEEEAKELTLDEWKAQQGQRIKPTFNIRKAGEGEDTTQWKKMVVLTSNKKKENDSEEELEYDPALYPQRVGRQQRVLDIQFNFNDGRRGGPGGFGGRGGRGGPRPGGFGGGPRSEGGNRDGGNREGGRDNREGGNRGPRDGQQHNNEGGGSSAQNQRPPIDRRGPGNNQNNNQNSGPGPNKRFERQQNTAPKVNDERQFPTLA

Target ORF information:

RefSeq Version	NM_165102.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa intronic gene (vig), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165102.2

ORF Insert Sequence:

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vig ( NM_165101.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723897.1 Drosophila melanogaster vasa intronic gene (vig), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15892
Clone ID Related Accession (Same CDS sequence)	NM_165102.2 , NM_165101.2 , NM_078848.3 , NM_165100.2
Accession Version	NM_165101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa intronic gene, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165101.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723897.1
CDS	146..1618
Translation	MDSAGKNRYELLFMDDDVSDPLDNLVAPTAAAAAAAAGKKKQPSAAAAAATKTTANKVANSNNKANAGSNIGGPNAKKPNQAEKENKPNNALNKTDGKKFTPSADNKQQQFNNNASSNYKQQGAPRQGGGANRTREFGSGQGQGQGQGGQQQRSVNFRQQNGNAETREQRNNRRNVRENVGAPDGQQSRPYRGPGGGPGAGGDRPQRQNRNYDGQNRKREFDRQSGSDRTGVKSIDKRDGAGSHNWGSVKEAIDDVNKNESETNVTNAEGGAKADESGTEPQNEQATAEEEAKELTLDEWKAQQGQRIKPTFNIRKAGEGEDTTQWKKMVVLTSNKKKENDSEEELEYDPALYPQRVGRQQRVLDIQFNFNDGRRGGPGGFGGRGGRGGPRPGGFGGGPRSEGGNRDGGNREGGRDNREGGNRGPRDGQQHNNEGGGSSAQNQRPPIDRRGPGNNQNNNQNSGPGPNKRFERQQNTAPKVNDERQFPTLA

Target ORF information:

RefSeq Version	NM_165101.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa intronic gene (vig), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165101.2

ORF Insert Sequence:

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vig ( NM_078848.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523572.1 Drosophila melanogaster vasa intronic gene (vig), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15892
Clone ID Related Accession (Same CDS sequence)	NM_165102.2 , NM_165101.2 , NM_078848.3 , NM_165100.2
Accession Version	NM_078848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa intronic gene, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078848.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523572.1
CDS	226..1698
Translation	MDSAGKNRYELLFMDDDVSDPLDNLVAPTAAAAAAAAGKKKQPSAAAAAATKTTANKVANSNNKANAGSNIGGPNAKKPNQAEKENKPNNALNKTDGKKFTPSADNKQQQFNNNASSNYKQQGAPRQGGGANRTREFGSGQGQGQGQGGQQQRSVNFRQQNGNAETREQRNNRRNVRENVGAPDGQQSRPYRGPGGGPGAGGDRPQRQNRNYDGQNRKREFDRQSGSDRTGVKSIDKRDGAGSHNWGSVKEAIDDVNKNESETNVTNAEGGAKADESGTEPQNEQATAEEEAKELTLDEWKAQQGQRIKPTFNIRKAGEGEDTTQWKKMVVLTSNKKKENDSEEELEYDPALYPQRVGRQQRVLDIQFNFNDGRRGGPGGFGGRGGRGGPRPGGFGGGPRSEGGNRDGGNREGGRDNREGGNRGPRDGQQHNNEGGGSSAQNQRPPIDRRGPGNNQNNNQNSGPGPNKRFERQQNTAPKVNDERQFPTLA

Target ORF information:

RefSeq Version	NM_078848.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa intronic gene (vig), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078848.3

ORF Insert Sequence:

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vig ( NM_165100.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723896.1 Drosophila melanogaster vasa intronic gene (vig), transcript variant B, mRNA.

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CloneID	OFa15892
Clone ID Related Accession (Same CDS sequence)	NM_165102.2 , NM_165101.2 , NM_078848.3 , NM_165100.2
Accession Version	NM_165100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa intronic gene, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165100.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723896.1
CDS	189..1661
Translation	MDSAGKNRYELLFMDDDVSDPLDNLVAPTAAAAAAAAGKKKQPSAAAAAATKTTANKVANSNNKANAGSNIGGPNAKKPNQAEKENKPNNALNKTDGKKFTPSADNKQQQFNNNASSNYKQQGAPRQGGGANRTREFGSGQGQGQGQGGQQQRSVNFRQQNGNAETREQRNNRRNVRENVGAPDGQQSRPYRGPGGGPGAGGDRPQRQNRNYDGQNRKREFDRQSGSDRTGVKSIDKRDGAGSHNWGSVKEAIDDVNKNESETNVTNAEGGAKADESGTEPQNEQATAEEEAKELTLDEWKAQQGQRIKPTFNIRKAGEGEDTTQWKKMVVLTSNKKKENDSEEELEYDPALYPQRVGRQQRVLDIQFNFNDGRRGGPGGFGGRGGRGGPRPGGFGGGPRSEGGNRDGGNREGGRDNREGGNRGPRDGQQHNNEGGGSSAQNQRPPIDRRGPGNNQNNNQNSGPGPNKRFERQQNTAPKVNDERQFPTLA

Target ORF information:

RefSeq Version	NM_165100.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa intronic gene (vig), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165100.2

ORF Insert Sequence:

ATGGACAGCG CCGGTAAAAA TCGTTATGAA CTTTTGTTCA TGGACGACGA TGTCAGCGAT 
CCATTAGATA ATCTTGTGGC GCCGACGGCC GCTGCTGCCG CAGCAGCAGC TGGCAAGAAG 
AAGCAACCGT CAGCTGCTGC TGCAGCGGCC ACCAAAACGA CCGCCAACAA GGTGGCCAAC 
AGCAATAACA AGGCGAACGC AGGATCCAAT ATTGGTGGAC CCAATGCCAA GAAACCAAAT 
CAGGCCGAAA AGGAGAACAA GCCCAATAAT GCGTTAAACA AAACCGATGG CAAGAAGTTC 
ACTCCATCGG CCGACAACAA ACAGCAGCAG TTCAATAATA ACGCCAGCAG CAATTACAAA 
CAACAGGGTG CTCCACGGCA AGGAGGTGGC GCCAACCGAA CACGTGAATT TGGTAGCGGT 
CAAGGACAGG GTCAAGGACA AGGTGGCCAG CAGCAGCGCA GCGTAAACTT CCGTCAACAG 
AATGGCAATG CCGAGACTCG TGAGCAGCGC AACAATCGCC GCAATGTCCG CGAAAACGTC 
GGAGCACCCG ATGGCCAGCA GTCGCGACCT TACCGCGGAC CAGGTGGAGG CCCAGGTGCT 
GGCGGAGATC GTCCCCAGCG TCAGAACCGC AACTACGATG GCCAGAACAG GAAGCGCGAG 
TTCGACCGCC AGTCGGGTTC CGATAGAACT GGTGTGAAGT CAATTGACAA ACGCGATGGA 
GCTGGCTCCC ACAACTGGGG ATCGGTCAAG GAGGCTATTG ATGATGTGAA CAAGAACGAA 
AGCGAAACCA ATGTGACCAA TGCCGAGGGC GGCGCCAAGG CCGATGAATC TGGCACTGAA 
CCACAAAACG AGCAGGCTAC CGCCGAGGAG GAAGCCAAAG AGCTGACCTT GGACGAGTGG 
AAGGCACAGC AAGGACAGCG CATCAAGCCA ACCTTTAATA TCCGCAAGGC TGGAGAAGGT 
GAGGACACCA CACAATGGAA GAAAATGGTC GTCCTAACTA GCAACAAGAA GAAGGAGAAC 
GACAGCGAAG AGGAGCTCGA GTACGACCCT GCCCTTTATC CACAGCGTGT GGGCAGGCAA 
CAGCGCGTCC TGGACATTCA GTTCAATTTC AACGATGGTC GTCGTGGCGG ACCTGGCGGT 
TTCGGTGGAC GCGGTGGCCG TGGAGGCCCC AGACCCGGTG GATTTGGAGG CGGACCACGC 
AGCGAGGGTG GCAACCGCGA TGGCGGCAAT CGTGAGGGCG GCCGAGATAA TCGTGAGGGT 
GGAAACCGTG GCCCACGGGA TGGCCAGCAG CATAATAACG AGGGCGGCGG TTCATCCGCT 
CAAAATCAAA GGCCACCCAT CGATCGTCGC GGTCCGGGAA ACAACCAGAA CAACAATCAG 
AACAGCGGTC CAGGGCCGAA TAAGCGTTTT GAGCGACAAC AGAACACCGC TCCCAAGGTC 
AACGATGAGC GTCAGTTTCC CACTCTGGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vig cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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