Pka-C1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pka-C1
Entrez Gene ID	34284
Full Name	Protein kinase, cAMP-dependent, catalytic subunit 1
Synonyms	6353,C,CG4379,CdkA,Cos,Cos-1,Cos1,DC0,DCO,Dc0,Dco,Dcpk,Dmel\CG4379,PKA,PKA CI,PKA Cl,PKA-C1,PKAC1,PKAc,PKAcA,Pka,Pka-C,PkaC1,cos1,dPKA,dc0,dcO,dco,l(2)01272,l(2)cos[[1]],l(2)s4402,pKA,pkA,pka,pka C1,pka-C1,pka-c1
General protein information	Preferred Names Protein kinase, cAMP-dependent, catalytic subunit 1 Names CG4379 gene product from transcript CG4379-RB CG4379-PB CG4379-PC CG4379-PD Costal-1 PKA catalytic subunit Pka-C1-PB Pka-C1-PC Pka-C1-PD cAMP-dependent protein kinase cAMP-dependent protein kinase 1 catalytic subunit of PKA catalytic subunit of cAMP-dependent protein kinase costal protein kinase A protein kinase, cAMP-dependent, catalytic subunit 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30C5-30C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_205950.3	NP_995672.1	protein kinase, cAMP-dependent, catalytic subunit 1, isoform C
NM_164866.3	NP_723479.1	protein kinase, cAMP-dependent, catalytic subunit 1, isoform B
NM_057629.4	NP_476977.1	protein kinase, cAMP-dependent, catalytic subunit 1, isoform D

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PRKACB	NP_777010.1
Gallus gallus (chicken)	PRKACB	XP_422379.1
Danio rerio (zebrafish)	prkacb	XP_003197858.1
Xenopus tropicalis (tropical clawed frog)	prkacb	NP_001164667.1
Canis lupus familiaris (dog)	LOC479975	XP_537099.1
Rattus norvegicus (Norway rat)	Prkacb	NP_001071113.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001384	XP_321752.4
Caenorhabditis elegans (roundworm)	kin-1	NP_493605.1
Saccharomyces cerevisiae (baker's yeast)	TPK1	NP_012371.2
Pan troglodytes (chimpanzee)	PRKACB	XP_001137476.1
Magnaporthe oryzae (rice blast fungus)	MGG_06368	XP_003717206.1
Homo sapiens (human)	PRKACB	NP_891993.1
	PRKACB	XP_001105747.2
Mus musculus (house mouse)	Prkacb	NP_001157672.1
Danio rerio (zebrafish)	prkacbb	NP_001030148.1
Drosophila melanogaster (fruit fly)	Pka-C1	NP_995672.1
Neurospora crassa	NCU06240	XP_963091.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Early-onset sleep defects in mutated HTT Drosophila models of Huntington's disease reflect alterations of PKA/CREB signaling.
Title: Early-onset sleep defects in Drosophila models of Huntington's disease reflect alterations of PKA/CREB signaling.

Hedgehog signalling activation causes PKA to switch its substrates from Ci to Smo within the Hh signalling complex.
Title: Switch of PKA substrates from Cubitus interruptus to Smoothened in the Hedgehog signalosome complex.

tightly controlled dose-dependent intra-neuronal PKA activity levels are critical in determining the dendritic arbor complexity, one of the possible ways being through the regulation of organelle distribution.
Title: Developmental shaping of dendritic arbors in Drosophila relies on tightly regulated intra-neuronal activity of protein kinase A (PKA).

Alpha- NAC is a substrate of PKA following Parathyroid Hormone signaling.
Title: The PTH-G?s-protein kinase A cascade controls ?NAC localization to regulate bone mass.

that manipulations that increase cAMP/PKA signaling in adipokinetic hormone (AKH)-producing cells at the base of the neuroendocrine ring gland restore the dNf1 growth deficiency.
Title: Genetic and functional studies implicate synaptic overgrowth and ring gland cAMP/PKA signaling defects in the Drosophila melanogaster neurofibromatosis-1 growth deficiency.

The following Pka-C1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pka-C1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14217	NM_164866.3 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant B (Pka-C1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14217	NM_057629.4 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant D (Pka-C1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14217	NM_205950.3 Latest version!	Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant C (Pka-C1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pka-C1 ( NM_205950.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995672.1 Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant C (Pka-C1), mRNA.

Price & Availability↑

CloneID	OFa14217
Clone ID Related Accession (Same CDS sequence)	NM_205950.3 , NM_164866.3 , NM_057629.4
Accession Version	NM_205950.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, catalytic subunit 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205950.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAACA ACGCCACCAC GTCGAATAAG AAGGTCGATG CCGCCGAGAC GGTGAAGGAG 
TTCCTCGAGC AGGCCAAGGA GGAGTTCGAG GACAAGTGGC GACGCAATCC GACCAACACC 
GCCGCCCTCG ATGACTTCGA ACGGATCAAG ACCCTGGGCA CCGGCTCCTT TGGCCGTGTC 
ATGATCGTCC AGCACAAGCC CACGAAAGAC TATTATGCCA TGAAGATCCT CGACAAGCAG 
AAGGTGGTCA AGCTGAAGCA GGTGGAGCAC ACGCTGAACG AGAAGCGAAT CCTGCAGGCC 
ATTCAGTTCC CCTTCCTCGT CTCGCTGCGC TACCACTTCA AGGACAACTC CAACCTTTAC 
ATGGTGCTGG AGTATGTTCC CGGTGGCGAG ATGTTCTCCC ACCTGCGCAA GGTGGGCCGC 
TTCTCGGAGC CGCACTCGCG CTTCTACGCG GCGCAAATCG TGCTGGCCTT CGAGTACCTG 
CACTACTTGG ACCTCATCTA CCGTGATCTG AAGCCGGAGA ATCTGCTGAT TGACTCGCAG 
GGCTACCTCA AGGTGACGGA CTTCGGTTTT GCCAAGCGGG TCAAGGGACG CACCTGGACA 
CTGTGCGGCA CGCCCGAATA CCTGGCCCCG GAGATCATTC TGTCCAAGGG CTATAACAAG 
GCCGTGGACT GGTGGGCGTT GGGCGTACTC GTCTACGAAA TGGCCGCCGG CTATCCGCCG 
TTCTTTGCGG ATCAGCCGAT TCAGATCTAC GAGAAGATCG TCTCCGGCAA GGTGCGCTTC 
CCATCGCACT TTGGCTCCGA TCTGAAGGAC CTACTGCGCA ACCTGCTGCA GGTGGATCTG 
ACCAAGCGCT ACGGCAATCT GAAGGCGGGC GTCAATGATA TTAAGAACCA GAAGTGGTTC 
GCCTCCACCG ACTGGATTGC GATCTTCCAA AAGAAAATCG AGGCGCCGTT CATTCCGCGG 
TGTAAGGGTC CCGGCGACAC GAGCAATTTC GATGACTACG AGGAGGAGGC GCTGCGGATC 
TCCAGCACCG AGAAGTGTGC CAAGGAGTTT GCTGAATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995672.1
CDS	1049..2110
Translation	MGNNATTSNKKVDAAETVKEFLEQAKEEFEDKWRRNPTNTAALDDFERIKTLGTGSFGRVMIVQHKPTKDYYAMKILDKQKVVKLKQVEHTLNEKRILQAIQFPFLVSLRYHFKDNSNLYMVLEYVPGGEMFSHLRKVGRFSEPHSRFYAAQIVLAFEYLHYLDLIYRDLKPENLLIDSQGYLKVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFGSDLKDLLRNLLQVDLTKRYGNLKAGVNDIKNQKWFASTDWIAIFQKKIEAPFIPRCKGPGDTSNFDDYEEEALRISSTEKCAKEFAEF

Target ORF information:

RefSeq Version	NM_205950.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant C (Pka-C1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205950.3

ORF Insert Sequence:

ATGGGCAACA ACGCCACCAC GTCGAATAAG AAGGTCGATG CCGCCGAGAC GGTGAAGGAG 
TTCCTCGAGC AGGCCAAGGA GGAGTTCGAG GACAAGTGGC GACGCAATCC GACCAACACC 
GCCGCCCTCG ATGACTTCGA ACGGATCAAG ACCCTGGGCA CCGGCTCCTT TGGCCGTGTC 
ATGATCGTCC AGCACAAGCC CACGAAAGAC TATTATGCCA TGAAGATCCT CGACAAGCAG 
AAGGTGGTCA AGCTGAAGCA GGTGGAGCAC ACGCTGAACG AGAAGCGAAT CCTGCAGGCC 
ATTCAGTTCC CCTTCCTCGT CTCGCTGCGC TACCACTTCA AGGACAACTC CAACCTTTAC 
ATGGTGCTGG AGTATGTTCC CGGTGGCGAG ATGTTCTCCC ACCTGCGCAA GGTGGGCCGC 
TTCTCGGAGC CGCACTCGCG CTTCTACGCG GCGCAAATCG TGCTGGCCTT CGAGTACCTG 
CACTACTTGG ACCTCATCTA CCGTGATCTG AAGCCGGAGA ATCTGCTGAT TGACTCGCAG 
GGCTACCTCA AGGTGACGGA CTTCGGTTTT GCCAAGCGGG TCAAGGGACG CACCTGGACA 
CTGTGCGGCA CGCCCGAATA CCTGGCCCCG GAGATCATTC TGTCCAAGGG CTATAACAAG 
GCCGTGGACT GGTGGGCGTT GGGCGTACTC GTCTACGAAA TGGCCGCCGG CTATCCGCCG 
TTCTTTGCGG ATCAGCCGAT TCAGATCTAC GAGAAGATCG TCTCCGGCAA GGTGCGCTTC 
CCATCGCACT TTGGCTCCGA TCTGAAGGAC CTACTGCGCA ACCTGCTGCA GGTGGATCTG 
ACCAAGCGCT ACGGCAATCT GAAGGCGGGC GTCAATGATA TTAAGAACCA GAAGTGGTTC 
GCCTCCACCG ACTGGATTGC GATCTTCCAA AAGAAAATCG AGGCGCCGTT CATTCCGCGG 
TGTAAGGGTC CCGGCGACAC GAGCAATTTC GATGACTACG AGGAGGAGGC GCTGCGGATC 
TCCAGCACCG AGAAGTGTGC CAAGGAGTTT GCTGAATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pka-C1 ( NM_164866.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723479.1 Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant B (Pka-C1), mRNA.

Price & Availability↑

CloneID	OFa14217
Clone ID Related Accession (Same CDS sequence)	NM_205950.3 , NM_164866.3 , NM_057629.4
Accession Version	NM_164866.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, catalytic subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164866.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAACA ACGCCACCAC GTCGAATAAG AAGGTCGATG CCGCCGAGAC GGTGAAGGAG 
TTCCTCGAGC AGGCCAAGGA GGAGTTCGAG GACAAGTGGC GACGCAATCC GACCAACACC 
GCCGCCCTCG ATGACTTCGA ACGGATCAAG ACCCTGGGCA CCGGCTCCTT TGGCCGTGTC 
ATGATCGTCC AGCACAAGCC CACGAAAGAC TATTATGCCA TGAAGATCCT CGACAAGCAG 
AAGGTGGTCA AGCTGAAGCA GGTGGAGCAC ACGCTGAACG AGAAGCGAAT CCTGCAGGCC 
ATTCAGTTCC CCTTCCTCGT CTCGCTGCGC TACCACTTCA AGGACAACTC CAACCTTTAC 
ATGGTGCTGG AGTATGTTCC CGGTGGCGAG ATGTTCTCCC ACCTGCGCAA GGTGGGCCGC 
TTCTCGGAGC CGCACTCGCG CTTCTACGCG GCGCAAATCG TGCTGGCCTT CGAGTACCTG 
CACTACTTGG ACCTCATCTA CCGTGATCTG AAGCCGGAGA ATCTGCTGAT TGACTCGCAG 
GGCTACCTCA AGGTGACGGA CTTCGGTTTT GCCAAGCGGG TCAAGGGACG CACCTGGACA 
CTGTGCGGCA CGCCCGAATA CCTGGCCCCG GAGATCATTC TGTCCAAGGG CTATAACAAG 
GCCGTGGACT GGTGGGCGTT GGGCGTACTC GTCTACGAAA TGGCCGCCGG CTATCCGCCG 
TTCTTTGCGG ATCAGCCGAT TCAGATCTAC GAGAAGATCG TCTCCGGCAA GGTGCGCTTC 
CCATCGCACT TTGGCTCCGA TCTGAAGGAC CTACTGCGCA ACCTGCTGCA GGTGGATCTG 
ACCAAGCGCT ACGGCAATCT GAAGGCGGGC GTCAATGATA TTAAGAACCA GAAGTGGTTC 
GCCTCCACCG ACTGGATTGC GATCTTCCAA AAGAAAATCG AGGCGCCGTT CATTCCGCGG 
TGTAAGGGTC CCGGCGACAC GAGCAATTTC GATGACTACG AGGAGGAGGC GCTGCGGATC 
TCCAGCACCG AGAAGTGTGC CAAGGAGTTT GCTGAATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723479.1
CDS	851..1912
Translation	MGNNATTSNKKVDAAETVKEFLEQAKEEFEDKWRRNPTNTAALDDFERIKTLGTGSFGRVMIVQHKPTKDYYAMKILDKQKVVKLKQVEHTLNEKRILQAIQFPFLVSLRYHFKDNSNLYMVLEYVPGGEMFSHLRKVGRFSEPHSRFYAAQIVLAFEYLHYLDLIYRDLKPENLLIDSQGYLKVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFGSDLKDLLRNLLQVDLTKRYGNLKAGVNDIKNQKWFASTDWIAIFQKKIEAPFIPRCKGPGDTSNFDDYEEEALRISSTEKCAKEFAEF

Target ORF information:

RefSeq Version	NM_164866.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant B (Pka-C1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164866.3

ORF Insert Sequence:

ATGGGCAACA ACGCCACCAC GTCGAATAAG AAGGTCGATG CCGCCGAGAC GGTGAAGGAG 
TTCCTCGAGC AGGCCAAGGA GGAGTTCGAG GACAAGTGGC GACGCAATCC GACCAACACC 
GCCGCCCTCG ATGACTTCGA ACGGATCAAG ACCCTGGGCA CCGGCTCCTT TGGCCGTGTC 
ATGATCGTCC AGCACAAGCC CACGAAAGAC TATTATGCCA TGAAGATCCT CGACAAGCAG 
AAGGTGGTCA AGCTGAAGCA GGTGGAGCAC ACGCTGAACG AGAAGCGAAT CCTGCAGGCC 
ATTCAGTTCC CCTTCCTCGT CTCGCTGCGC TACCACTTCA AGGACAACTC CAACCTTTAC 
ATGGTGCTGG AGTATGTTCC CGGTGGCGAG ATGTTCTCCC ACCTGCGCAA GGTGGGCCGC 
TTCTCGGAGC CGCACTCGCG CTTCTACGCG GCGCAAATCG TGCTGGCCTT CGAGTACCTG 
CACTACTTGG ACCTCATCTA CCGTGATCTG AAGCCGGAGA ATCTGCTGAT TGACTCGCAG 
GGCTACCTCA AGGTGACGGA CTTCGGTTTT GCCAAGCGGG TCAAGGGACG CACCTGGACA 
CTGTGCGGCA CGCCCGAATA CCTGGCCCCG GAGATCATTC TGTCCAAGGG CTATAACAAG 
GCCGTGGACT GGTGGGCGTT GGGCGTACTC GTCTACGAAA TGGCCGCCGG CTATCCGCCG 
TTCTTTGCGG ATCAGCCGAT TCAGATCTAC GAGAAGATCG TCTCCGGCAA GGTGCGCTTC 
CCATCGCACT TTGGCTCCGA TCTGAAGGAC CTACTGCGCA ACCTGCTGCA GGTGGATCTG 
ACCAAGCGCT ACGGCAATCT GAAGGCGGGC GTCAATGATA TTAAGAACCA GAAGTGGTTC 
GCCTCCACCG ACTGGATTGC GATCTTCCAA AAGAAAATCG AGGCGCCGTT CATTCCGCGG 
TGTAAGGGTC CCGGCGACAC GAGCAATTTC GATGACTACG AGGAGGAGGC GCTGCGGATC 
TCCAGCACCG AGAAGTGTGC CAAGGAGTTT GCTGAATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pka-C1 ( NM_057629.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476977.1 Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant D (Pka-C1), mRNA.

Price & Availability↑

CloneID	OFa14217
Clone ID Related Accession (Same CDS sequence)	NM_205950.3 , NM_164866.3 , NM_057629.4
Accession Version	NM_057629.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cAMP-dependent, catalytic subunit 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057629.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAACA ACGCCACCAC GTCGAATAAG AAGGTCGATG CCGCCGAGAC GGTGAAGGAG 
TTCCTCGAGC AGGCCAAGGA GGAGTTCGAG GACAAGTGGC GACGCAATCC GACCAACACC 
GCCGCCCTCG ATGACTTCGA ACGGATCAAG ACCCTGGGCA CCGGCTCCTT TGGCCGTGTC 
ATGATCGTCC AGCACAAGCC CACGAAAGAC TATTATGCCA TGAAGATCCT CGACAAGCAG 
AAGGTGGTCA AGCTGAAGCA GGTGGAGCAC ACGCTGAACG AGAAGCGAAT CCTGCAGGCC 
ATTCAGTTCC CCTTCCTCGT CTCGCTGCGC TACCACTTCA AGGACAACTC CAACCTTTAC 
ATGGTGCTGG AGTATGTTCC CGGTGGCGAG ATGTTCTCCC ACCTGCGCAA GGTGGGCCGC 
TTCTCGGAGC CGCACTCGCG CTTCTACGCG GCGCAAATCG TGCTGGCCTT CGAGTACCTG 
CACTACTTGG ACCTCATCTA CCGTGATCTG AAGCCGGAGA ATCTGCTGAT TGACTCGCAG 
GGCTACCTCA AGGTGACGGA CTTCGGTTTT GCCAAGCGGG TCAAGGGACG CACCTGGACA 
CTGTGCGGCA CGCCCGAATA CCTGGCCCCG GAGATCATTC TGTCCAAGGG CTATAACAAG 
GCCGTGGACT GGTGGGCGTT GGGCGTACTC GTCTACGAAA TGGCCGCCGG CTATCCGCCG 
TTCTTTGCGG ATCAGCCGAT TCAGATCTAC GAGAAGATCG TCTCCGGCAA GGTGCGCTTC 
CCATCGCACT TTGGCTCCGA TCTGAAGGAC CTACTGCGCA ACCTGCTGCA GGTGGATCTG 
ACCAAGCGCT ACGGCAATCT GAAGGCGGGC GTCAATGATA TTAAGAACCA GAAGTGGTTC 
GCCTCCACCG ACTGGATTGC GATCTTCCAA AAGAAAATCG AGGCGCCGTT CATTCCGCGG 
TGTAAGGGTC CCGGCGACAC GAGCAATTTC GATGACTACG AGGAGGAGGC GCTGCGGATC 
TCCAGCACCG AGAAGTGTGC CAAGGAGTTT GCTGAATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476977.1
CDS	1264..2325
Translation	MGNNATTSNKKVDAAETVKEFLEQAKEEFEDKWRRNPTNTAALDDFERIKTLGTGSFGRVMIVQHKPTKDYYAMKILDKQKVVKLKQVEHTLNEKRILQAIQFPFLVSLRYHFKDNSNLYMVLEYVPGGEMFSHLRKVGRFSEPHSRFYAAQIVLAFEYLHYLDLIYRDLKPENLLIDSQGYLKVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFGSDLKDLLRNLLQVDLTKRYGNLKAGVNDIKNQKWFASTDWIAIFQKKIEAPFIPRCKGPGDTSNFDDYEEEALRISSTEKCAKEFAEF

Target ORF information:

RefSeq Version	NM_057629.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cAMP-dependent, catalytic subunit 1, transcript variant D (Pka-C1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057629.4

ORF Insert Sequence:

ATGGGCAACA ACGCCACCAC GTCGAATAAG AAGGTCGATG CCGCCGAGAC GGTGAAGGAG 
TTCCTCGAGC AGGCCAAGGA GGAGTTCGAG GACAAGTGGC GACGCAATCC GACCAACACC 
GCCGCCCTCG ATGACTTCGA ACGGATCAAG ACCCTGGGCA CCGGCTCCTT TGGCCGTGTC 
ATGATCGTCC AGCACAAGCC CACGAAAGAC TATTATGCCA TGAAGATCCT CGACAAGCAG 
AAGGTGGTCA AGCTGAAGCA GGTGGAGCAC ACGCTGAACG AGAAGCGAAT CCTGCAGGCC 
ATTCAGTTCC CCTTCCTCGT CTCGCTGCGC TACCACTTCA AGGACAACTC CAACCTTTAC 
ATGGTGCTGG AGTATGTTCC CGGTGGCGAG ATGTTCTCCC ACCTGCGCAA GGTGGGCCGC 
TTCTCGGAGC CGCACTCGCG CTTCTACGCG GCGCAAATCG TGCTGGCCTT CGAGTACCTG 
CACTACTTGG ACCTCATCTA CCGTGATCTG AAGCCGGAGA ATCTGCTGAT TGACTCGCAG 
GGCTACCTCA AGGTGACGGA CTTCGGTTTT GCCAAGCGGG TCAAGGGACG CACCTGGACA 
CTGTGCGGCA CGCCCGAATA CCTGGCCCCG GAGATCATTC TGTCCAAGGG CTATAACAAG 
GCCGTGGACT GGTGGGCGTT GGGCGTACTC GTCTACGAAA TGGCCGCCGG CTATCCGCCG 
TTCTTTGCGG ATCAGCCGAT TCAGATCTAC GAGAAGATCG TCTCCGGCAA GGTGCGCTTC 
CCATCGCACT TTGGCTCCGA TCTGAAGGAC CTACTGCGCA ACCTGCTGCA GGTGGATCTG 
ACCAAGCGCT ACGGCAATCT GAAGGCGGGC GTCAATGATA TTAAGAACCA GAAGTGGTTC 
GCCTCCACCG ACTGGATTGC GATCTTCCAA AAGAAAATCG AGGCGCCGTT CATTCCGCGG 
TGTAAGGGTC CCGGCGACAC GAGCAATTTC GATGACTACG AGGAGGAGGC GCTGCGGATC 
TCCAGCACCG AGAAGTGTGC CAAGGAGTTT GCTGAATTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Pka-C1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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