l(2)k09022 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(2)k09022
Entrez Gene ID	33960
Full Name	lethal (2) k09022
Synonyms	CG10805,Dmel\CG10805,anon-WO0118547.160
General protein information	Preferred Names lethal (2) k09022 Names CG10805 gene product from transcript CG10805-RA CG10805-PA l(2)k09022-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27C1-27C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135235.3	NP_609079.2	lethal (2) k09022

Pathways from BioSystems

Homologies

	HEATR1	XP_002802165.1
Rattus norvegicus (Norway rat)	Heatr1	NP_001101888.1
Gallus gallus (chicken)	HEATR1	XP_004935523.1
Mus musculus (house mouse)	Heatr1	NP_659084.3
Rattus norvegicus (Norway rat)	LOC102552190	XP_006254237.1
Danio rerio (zebrafish)	heatr1	NP_956194.1
Arabidopsis thaliana (thale cress)	AT3G06530	NP_001189829.1
Canis lupus familiaris (dog)	HEATR1	XP_536334.2
Danio rerio (zebrafish)	LOC100151449	XP_005156148.1
Pan troglodytes (chimpanzee)	HEATR1	XP_001157116.1
Drosophila melanogaster (fruit fly)	l(2)k09022	NP_609079.2
Caenorhabditis elegans (roundworm)	toe-1	NP_494782.1
Homo sapiens (human)	HEATR1	NP_060542.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009004	XP_319753.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(2)k09022 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(2)k09022 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16340	NM_135235.3 Latest version!	Drosophila melanogaster lethal (2) k09022 (l(2)k09022), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(2)k09022 ( NM_135235.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609079.2 Drosophila melanogaster lethal (2) k09022 (l(2)k09022), mRNA.

Price & Availability↑

CloneID	OFa16340
Clone ID Related Accession (Same CDS sequence)	NM_135235.3
Accession Version	NM_135235.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) k09022
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135235.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTACTG CGTTGGCACA GCAGCTGCAG AAACTGGCTG CGCCGCAGTC GTCGGTAACT 
TTGGCAGATG CACGAAGTCG CGCCTCCATA CTCTTCGATC CCAAGGAGGC GGCCACCAAG 
GATCGGCGGT CCATTTATGA GATCGGGTTA ACGGGACTGC AGGAGCTGAC CGACTTTAAT 
CCGGCCTTCA AGGAGTTCCA GCTAACGTTG TTCGATGAGG CCACATTGAC GCTGGAGCGT 
TCCGTGGAGC TGCCGGAGAT TAACAAGATG CTGGACGCGG CCATTGCAAA GTTTCTGCGC 
CTTCTTTCGC CGTACCTTCT GCTCCGCCCC GCACACATGG CCTTCGAATG GTTGCTGCGA 
CGATTCCAGG TTCACGAGTA TAACCGTAGC GAAGTTATGG CCCTGATTCT GCCCTATCAC 
GAGACAATGA TCTTTGTCCA GATCGTTAAG ACCATGAGGC TCCGGTCATC GGATGGCGAC 
TGGTACTGGC TGCGCCCGTT GCAGCGTCCT GGCGTTCCGC TAGCCAAGAC TGCTATTATT 
AACAGAGCCG CCAGCAATCC CGCCTTCCTC GGCTTCATCT GCCAAAGTAC CCAGAAGGCG 
GTTAAGGAAC TGGGTCCTCG AGCCCACCAA CTGCAAGCCC AGATCAACTT CTATGCCACC 
GTCGTAGTTG GCGCCCTGCA AACGGCCAAA CCACTGCAGG ACTGGCACAT CACCACTATT 
CTGGAGTCGC TGCTTCGTGG CCTGATTTCC GATAATATCG ACTTTATGGC GGCCGCCTAT 
GTGATCGTTG CTCAGCTTGT TTCCCGCACC AAGCTCAAGA GCAAGGTGTG CAATGCCCTG 
CTGGAACGCG TGGCCAACTG CCCGTTTGAG AGGCTTCACA GTGAGTCGCT GCTCCTGCTT 
GTCTGCATCT ATGGCAAACA GCAAGCCGCA TTGCCGCACT TTAAGCCGGA AACCATACTC 
AATCTGGTTG GGAAGAAGTG GCTTATCTCC ACTCTGTCCT CCCTGGCCAA AGGCAACATC 
GCCATCCAGT CTATCTGCAT GCCACTCATG ACCGGAGCCG TGGCCGCCAT TCGGGACGAT 
GACGCCTCTT CTAACTCATG CAAACTGTTC TTGGACAACC TGCTGTCAGA GGTGCCAATG 
CCAAAGCCGA CTGCTCAGCA ACTGATCAAT TGCTTTTTGG ATACTTATGT AGAGACTGCG 
ATCGATGCAC CAGAGCCCAT GGAAACTAAC TCAAACGAAG ATGACGATAC CATTGTAATT 
GATTCGGATG ATGAGATCGA GACGGAAAAG ACCACCTTTC AAGCCTGGTA TTCCACTTAC 
CTCGAGAAGC TGGAGAGACG CTATCCAGAA GCTTTTGATC TGAGCGTTAA AGAGGCCTTG 
AGATCGAAAT CCTCCACAAG CAATCGCCAG AAGGCCTTAA AGTTGGCTTT GGGCTTCCGT 
CTCAACACTA CTGATGAAAA AGCGAAGCAC GCCTACGAAA AACTATACCA CTACAGCGCT 
GACTGGCGAC TTAGTGCTGT TCAGAAACTA CTCCAGAATT TGAATGTGAC TAAAAAGCGG 
GAGAGAAGCG TTAAGCTTCT GCAGGAATGT CTGCCCGATA GGATAAATGA TGATAGCGGT 
GCTGTGGTCT CAACCTTGCT ATCATTGCCT ACCGAAGAGC TCGCCGAGAT GTTGGGTCCG 
TTGCCTCTGG CTCAAACCCT ATGTCATCTG CTGTATCGTG CTCAGTCCGA AAAGGATGAG 
GAATGGCAAC CTGTGGTTCC CCTCGCTGTT CGTCACTTGA CCTCTGCCTT GGTGAGCGGA 
AGCTATGACA CAAATTTGGT GCTATTAGCG CTGATGCCGC TTCTCTTCCC TGGCGAAGCA 
TTAGCTGAAC ATCAGCACAA GGCTCTGCGG ATTCTACTTG GCAGTGATTT TGTCAGTAAA 
GTTCCGTTCT TGGCAGAACT TAAAGTGAGC AACAAATTCA GCGACTTTAA TGTCGGTGAG 
CACCGTCAGC ACTTCCTGGA CATCATAGCG AGCAGCAATC AGGAACTGAG TAGTCAGGAA 
CGTGCGTTGC TCCAAAGCGT AGAAGATCAT GGCGGTGAGT TGTACATTCA GAAAGCCTCC 
CAGCTGACGC ACCTGTTACT CCTGCTAACT GCCTACGCCA AGAGGGAACT ACAGCCTCGG 
GAAAGCCTTC ACATGCTGGA GAAAATTGGG CTTTATAGTC GGCGCCTCCA GTTCCGTGTC 
GTCAATGGTA GTCAAAACAC ACAGAATTGT GCACCTCTAC AATTGTATGT GGATTTCCTA 
TTGACTTTGG TTAAGAACAC CAAATGGACA GCTCTAGCAT CCACTCCTTG GAATCAGATG 
ACCGATGAGC TTCGCCTCTG TCTGCGCCTG CTGGAAATAA TATGCGCCCA AGTGTTTTCG 
GAAAAAGCTG ATCAGCCAGA GCGTCAGGAG TGGACACGAG CCCTGCAGCA AAGTTTGCAA 
CTAATCCTGC CTGAAGCTCA AGACAGATTG GAAGTGCTAT CAAACTTCTA TGTGTTCGAG 
CGACTGCCAG AATTGTGGCC TCGAGACTCT GACTATGCCG TGTTCCGGTT GCAAGGGTTT 
ATTATTCTAG AAGCGGTGCT GTCCAATCCG AAATCCCAAA TAGACTGCGG TCTGGTTCAT 
GTGCTGCGGG TGGCCAACGC CTGTGGATCT CCCTTACAAA CTCTGCGAGT GCAGGCCATT 
AACATTCTGC AATTAATCAG TAATCGCAAG CTGGTGAGCC ATGTGGAGCA ATTGGTGCGT 
TCGCTGCTGC AGCGCAAGAG TGAGTTGAGC ATGGATCATG AACAATATGC CCTGATTCTA 
TACACCATTC TGGAACCAGA GAAGGCAACT GCCAAGGAAA GACTCGTGCT GTCAAAACTG 
AAACGATCTG TTTTGGCTTT GGCCTCCGAT CCGAAGCAGT CTCCCATTTG TACGGCGTCT 
TTGCTTGCGG CCCTAAAGCA CGTCAACGAT GAGAATTTCT TAAACGAATT GCTGCCGCTG 
GGTCTTGACT CCCTGAAGAC GATAACTGCG GGAGAAGACA ACCAGAATAT AAAACAGCTG 
CCATGGCCAC ATAGCGAAAT CTACAAGTCG GTGATAGAAA GGTTTGAGGG GCGTGTTGCG 
CTGAATGTAT TGCTGCGAAA GGATCTGGCT TGGAAATTGT TTGAAGACAG CTTCGCCCAG 
TATGACACCT ATGTCCAACT TGAACAGAAG CTACAGCCTC TTCCCTGCGT CCTGCTAAAC 
AGCCTCACGC CGGAGACCTT TGAGCAAATG CATGCCAAGC ACAAGATTGC TCTAATCAAG 
CTGATAGTGG AGTCGGCTAC CAATTCGGAC AATGACAGCA TATTCTTGGC TAGCCATCGT 
TTGCTGAAGC GCTGCCGTTT GGATTGCCAG CCACTCGTAC CGATCCTGTT GGAGATGGCC 
AACACCAAGG TGGAGAAGAA ACAACCCGTC AAACGACGAT CAGTACAGGC AACCCAACTG 
GATCTAACCA GCCCATACTG GAAGCAGGGA ATGACCCTTC TCGAGCTGTT GGAGCACAAG 
AAACAGCTGG TTGGAGCGGA GCTGCTGATC CCACCGCTCT TCGAACTTCT ACAGGCCTGT 
CTTACGATGG AAGAGCATAG TGCCGCCGAG TATCCCAAGC AGCTGATTCT CTCAAGCTTG 
CTGCACTGCT GTCAGACGGC ACAGTCGGCC GGTGTGCAGC TCGTTAAGGC TATGCCTGAA 
TCTAGCTTCC GCATCGAGCT GGTGGTGCAA TCCTTGAGGA ATACCCGAAA TCCGCAGACC 
CAGCAGCATG CGCTGCTTTT CCTGACTCAC TGTGCTGGCA TGTATCCGCA ACAAGTTCTA 
CACAAGATCG TTGAAATCTT TACGTTCGTT GGATCCACGG TGGCGCGACA CGACGATGCC 
TTCAGCCTGC ATATAATTCA CAATGTGGTG GAGTCGATTA TACCCATTCT GCTTCTGAAT 
ACCGGACATA ATGAGCTGGT TATACCCGTG CTTAAGGTGT TCGCTGATAT TTGCACGGAC 
GTGCCGGTCC ATCGGAGATT GCCATTGTAT GCTACCCTGT TCCGAGTTCT GGAGCCCAAG 
GAGCACCTTT GGCAGTTCTT GTGCATTATC TTTGAGTCTC AAGTGCTTCT GGAGCAAGTT 
CCACAAAAAG TGTCCACAGA CAAGTCGCGG CTGGACTTTG CCCGTGAACT GACGTTGATG 
TTCGAAGATC CGACGGTCGC AATTCAGACG TGCATCCGTC TATTGGATTA CTTGGCCAAG 
TTGCCGGCAA CCAAAAGCAG TCTTTCGGGT GGATCAGGCT CCTCTGTGCT AAGCACGGAA 
CAACAGCTCT TTGATGTTCG GACACGGACG TTCAAGCAAC TGCGGCACTA CAAGTACCTC 
ATCATGGACT TCCTTTCTGG AATTAGCAGC TGCAACGAAT GGGAGAAGAA GATGAAGCGT 
CCTGATCCCA ACGAGCTACT TCCGTATTAT CAGGAATTCA TCCTAAAGAC ACTCGCGTAT 
GTGGGCGTAC TTAATGGAGC ATTGGAAGCA GCCTCCGAAA CGCCTTCTCT GGAAAAGTTC 
TGGCGCGTGC TAGCTAACCA TGCACACGAT GTCTTGGATA ACGCCATCGG TCTTTTGGCA 
CCGCAACATT TCATCAGTGT GATAACCGAG CTACTGAAGC ATGATCATGT GTATGTGCGC 
ATTAAGGTGA TGGACTTGTT GGTGACCAAG CTAAGTCCGT CGAGCGATTA CTTCCAGCAA 
TCCAACGCTG AACACTTTGG AGTGCTTTTC GCTCCTTTGC AAGAGATTAT CAACGGAATC 
CTCGAGGGCA GTTCCAACAG TGCGCAGCAG GCGAAGCTTC AACAGACTGC GTTGCATGCC 
CTACAGTTGT TGGCCCTCCG TCACGGACGC GACTACATCG AGGAGTGTCG CTCTCTTCTG 
GCCACCTTGA CCAAGATCAC CAAACGACGT GCGAACGTGC CCAAGGCGGT AGTGGGCAAT 
GTGGTGCTGA CCCTCGTCGA AATTTGCGCA AGTCTTAAGG CCCATGCCTT GGCCCAATTG 
CCCAAGTTCG CTCCCCAACT TACTGAGTTA CTGAAGGAAC AGGTTCACCA AATGGCCTCC 
CTCAAACAAG GACCAGATTA CGTCTGTTCC ACCCTTGTCA CAGCACTCCA CAAGCTCTTC 
AAGGCGTTGC CGCTTTTCTT GGGACCCTAC TTGGTGGACA TTATCGGAGG CTTGGCGCGC 
CTTTCAGTGC AATTGGAAAA TCCACAGCTG CTGCAGGATA AGCGCACACA GGTGCTTAAG 
CAGAAACTTG CGGACGTTTG GAGTGCAGTT GCCCAGGGCG TCGAGGTGCG CATTCTTGTG 
CCCAGTTGCG CCAAGGCGTT CTCCAGTCTA TTAGAGCAGC AAGCGTACGA CGAGCTAGGA 
CATCTAATGC AGCAGCTGTT GCTGCAGAGC GTCCGACACA ATTCAGCCGC TCAATTGCAA 
CCGGTTCAGG ATCCCCTAAG CGAGCTCTTC CTGCAAGCAC TCAACTTCCG GCTGCAAGTG 
CGTGGACTTG GTTTGCAGAG GCAATTGGTT TCGGATGTAG AGGCTTCCAT AACCGAGACC 
TTTGTGACGT GGATCCTAAA GTTGTCAGAG ACCAGTTTCC GTCCCATGTA CTCTCGGGTA 
CATAAGTGGG CACTGGAGAG TACCTCTCGG GAAACGCGTC TCACCTACTT CCTGCTGACC 
AACCGGATCG CCGAAGCTCT AAAATCTTTG TTCGTGCTAT TTGCCAGCGA TTTCGTGGAA 
GACTCTTCGC GCTTGCTGAC GGAGCACAAC TCAATCCGTC CCGAGTTCGA AGTCGAAGAA 
AGGGAAGATG ATGTGGACCT GTTAATGGCA ATACTGAACA CTCTGCATCA TGTGTTCTTG 
TACTGCAGCG AGGACTTCAT TAATGACCAC CGCTTCAACG TGTTAATGCC ACCGCTGGTC 
AACCAATTAG AAAACGACCT GGTATTGGGA AATGAGTCGC TTCAGCAAGT TCTCAGTAAC 
TGCATTGCCC AGTTTGCGGT GGCCACCAAC GATGTGATGT GGAAGCAGCT CAACAGCCAA 
GTGCTCCTAA AGACACGCAC ATCCAATCCC GAAGTGCGGA TTCTGGCATT CAACAGTTGT 
GTGGCTATCG CGCGCAAACT GGGTGAAAGC TATGCCGCCC TGTTGCCAGA GACAGTTCCA 
TTCATCGCTG AGCTCCTGGA GGACGAGCAT CAACGTGTGG AGAAGAACAC CCGGACTGGA 
GTGCAGGAGC TAGAAACTAT TTTAGGCGAG TCTGTGCAAA AGTATCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609079.2
CDS	105..6395
Translation	MSTALAQQLQKLAAPQSSVTLADARSRASILFDPKEAATKDRRSIYEIGLTGLQELTDFNPAFKEFQLTLFDEATLTLERSVELPEINKMLDAAIAKFLRLLSPYLLLRPAHMAFEWLLRRFQVHEYNRSEVMALILPYHETMIFVQIVKTMRLRSSDGDWYWLRPLQRPGVPLAKTAIINRAASNPAFLGFICQSTQKAVKELGPRAHQLQAQINFYATVVVGALQTAKPLQDWHITTILESLLRGLISDNIDFMAAAYVIVAQLVSRTKLKSKVCNALLERVANCPFERLHSESLLLLVCIYGKQQAALPHFKPETILNLVGKKWLISTLSSLAKGNIAIQSICMPLMTGAVAAIRDDDASSNSCKLFLDNLLSEVPMPKPTAQQLINCFLDTYVETAIDAPEPMETNSNEDDDTIVIDSDDEIETEKTTFQAWYSTYLEKLERRYPEAFDLSVKEALRSKSSTSNRQKALKLALGFRLNTTDEKAKHAYEKLYHYSADWRLSAVQKLLQNLNVTKKRERSVKLLQECLPDRINDDSGAVVSTLLSLPTEELAEMLGPLPLAQTLCHLLYRAQSEKDEEWQPVVPLAVRHLTSALVSGSYDTNLVLLALMPLLFPGEALAEHQHKALRILLGSDFVSKVPFLAELKVSNKFSDFNVGEHRQHFLDIIASSNQELSSQERALLQSVEDHGGELYIQKASQLTHLLLLLTAYAKRELQPRESLHMLEKIGLYSRRLQFRVVNGSQNTQNCAPLQLYVDFLLTLVKNTKWTALASTPWNQMTDELRLCLRLLEIICAQVFSEKADQPERQEWTRALQQSLQLILPEAQDRLEVLSNFYVFERLPELWPRDSDYAVFRLQGFIILEAVLSNPKSQIDCGLVHVLRVANACGSPLQTLRVQAINILQLISNRKLVSHVEQLVRSLLQRKSELSMDHEQYALILYTILEPEKATAKERLVLSKLKRSVLALASDPKQSPICTASLLAALKHVNDENFLNELLPLGLDSLKTITAGEDNQNIKQLPWPHSEIYKSVIERFEGRVALNVLLRKDLAWKLFEDSFAQYDTYVQLEQKLQPLPCVLLNSLTPETFEQMHAKHKIALIKLIVESATNSDNDSIFLASHRLLKRCRLDCQPLVPILLEMANTKVEKKQPVKRRSVQATQLDLTSPYWKQGMTLLELLEHKKQLVGAELLIPPLFELLQACLTMEEHSAAEYPKQLILSSLLHCCQTAQSAGVQLVKAMPESSFRIELVVQSLRNTRNPQTQQHALLFLTHCAGMYPQQVLHKIVEIFTFVGSTVARHDDAFSLHIIHNVVESIIPILLLNTGHNELVIPVLKVFADICTDVPVHRRLPLYATLFRVLEPKEHLWQFLCIIFESQVLLEQVPQKVSTDKSRLDFARELTLMFEDPTVAIQTCIRLLDYLAKLPATKSSLSGGSGSSVLSTEQQLFDVRTRTFKQLRHYKYLIMDFLSGISSCNEWEKKMKRPDPNELLPYYQEFILKTLAYVGVLNGALEAASETPSLEKFWRVLANHAHDVLDNAIGLLAPQHFISVITELLKHDHVYVRIKVMDLLVTKLSPSSDYFQQSNAEHFGVLFAPLQEIINGILEGSSNSAQQAKLQQTALHALQLLALRHGRDYIEECRSLLATLTKITKRRANVPKAVVGNVVLTLVEICASLKAHALAQLPKFAPQLTELLKEQVHQMASLKQGPDYVCSTLVTALHKLFKALPLFLGPYLVDIIGGLARLSVQLENPQLLQDKRTQVLKQKLADVWSAVAQGVEVRILVPSCAKAFSSLLEQQAYDELGHLMQQLLLQSVRHNSAAQLQPVQDPLSELFLQALNFRLQVRGLGLQRQLVSDVEASITETFVTWILKLSETSFRPMYSRVHKWALESTSRETRLTYFLLTNRIAEALKSLFVLFASDFVEDSSRLLTEHNSIRPEFEVEEREDDVDLLMAILNTLHHVFLYCSEDFINDHRFNVLMPPLVNQLENDLVLGNESLQQVLSNCIAQFAVATNDVMWKQLNSQVLLKTRTSNPEVRILAFNSCVAIARKLGESYAALLPETVPFIAELLEDEHQRVEKNTRTGVQELETILGESVQKYL

Target ORF information:

RefSeq Version	NM_135235.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) k09022 (l(2)k09022), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135235.3

ORF Insert Sequence:

ATGAGTACTG CGTTGGCACA GCAGCTGCAG AAACTGGCTG CGCCGCAGTC GTCGGTAACT 
TTGGCAGATG CACGAAGTCG CGCCTCCATA CTCTTCGATC CCAAGGAGGC GGCCACCAAG 
GATCGGCGGT CCATTTATGA GATCGGGTTA ACGGGACTGC AGGAGCTGAC CGACTTTAAT 
CCGGCCTTCA AGGAGTTCCA GCTAACGTTG TTCGATGAGG CCACATTGAC GCTGGAGCGT 
TCCGTGGAGC TGCCGGAGAT TAACAAGATG CTGGACGCGG CCATTGCAAA GTTTCTGCGC 
CTTCTTTCGC CGTACCTTCT GCTCCGCCCC GCACACATGG CCTTCGAATG GTTGCTGCGA 
CGATTCCAGG TTCACGAGTA TAACCGTAGC GAAGTTATGG CCCTGATTCT GCCCTATCAC 
GAGACAATGA TCTTTGTCCA GATCGTTAAG ACCATGAGGC TCCGGTCATC GGATGGCGAC 
TGGTACTGGC TGCGCCCGTT GCAGCGTCCT GGCGTTCCGC TAGCCAAGAC TGCTATTATT 
AACAGAGCCG CCAGCAATCC CGCCTTCCTC GGCTTCATCT GCCAAAGTAC CCAGAAGGCG 
GTTAAGGAAC TGGGTCCTCG AGCCCACCAA CTGCAAGCCC AGATCAACTT CTATGCCACC 
GTCGTAGTTG GCGCCCTGCA AACGGCCAAA CCACTGCAGG ACTGGCACAT CACCACTATT 
CTGGAGTCGC TGCTTCGTGG CCTGATTTCC GATAATATCG ACTTTATGGC GGCCGCCTAT 
GTGATCGTTG CTCAGCTTGT TTCCCGCACC AAGCTCAAGA GCAAGGTGTG CAATGCCCTG 
CTGGAACGCG TGGCCAACTG CCCGTTTGAG AGGCTTCACA GTGAGTCGCT GCTCCTGCTT 
GTCTGCATCT ATGGCAAACA GCAAGCCGCA TTGCCGCACT TTAAGCCGGA AACCATACTC 
AATCTGGTTG GGAAGAAGTG GCTTATCTCC ACTCTGTCCT CCCTGGCCAA AGGCAACATC 
GCCATCCAGT CTATCTGCAT GCCACTCATG ACCGGAGCCG TGGCCGCCAT TCGGGACGAT 
GACGCCTCTT CTAACTCATG CAAACTGTTC TTGGACAACC TGCTGTCAGA GGTGCCAATG 
CCAAAGCCGA CTGCTCAGCA ACTGATCAAT TGCTTTTTGG ATACTTATGT AGAGACTGCG 
ATCGATGCAC CAGAGCCCAT GGAAACTAAC TCAAACGAAG ATGACGATAC CATTGTAATT 
GATTCGGATG ATGAGATCGA GACGGAAAAG ACCACCTTTC AAGCCTGGTA TTCCACTTAC 
CTCGAGAAGC TGGAGAGACG CTATCCAGAA GCTTTTGATC TGAGCGTTAA AGAGGCCTTG 
AGATCGAAAT CCTCCACAAG CAATCGCCAG AAGGCCTTAA AGTTGGCTTT GGGCTTCCGT 
CTCAACACTA CTGATGAAAA AGCGAAGCAC GCCTACGAAA AACTATACCA CTACAGCGCT 
GACTGGCGAC TTAGTGCTGT TCAGAAACTA CTCCAGAATT TGAATGTGAC TAAAAAGCGG 
GAGAGAAGCG TTAAGCTTCT GCAGGAATGT CTGCCCGATA GGATAAATGA TGATAGCGGT 
GCTGTGGTCT CAACCTTGCT ATCATTGCCT ACCGAAGAGC TCGCCGAGAT GTTGGGTCCG 
TTGCCTCTGG CTCAAACCCT ATGTCATCTG CTGTATCGTG CTCAGTCCGA AAAGGATGAG 
GAATGGCAAC CTGTGGTTCC CCTCGCTGTT CGTCACTTGA CCTCTGCCTT GGTGAGCGGA 
AGCTATGACA CAAATTTGGT GCTATTAGCG CTGATGCCGC TTCTCTTCCC TGGCGAAGCA 
TTAGCTGAAC ATCAGCACAA GGCTCTGCGG ATTCTACTTG GCAGTGATTT TGTCAGTAAA 
GTTCCGTTCT TGGCAGAACT TAAAGTGAGC AACAAATTCA GCGACTTTAA TGTCGGTGAG 
CACCGTCAGC ACTTCCTGGA CATCATAGCG AGCAGCAATC AGGAACTGAG TAGTCAGGAA 
CGTGCGTTGC TCCAAAGCGT AGAAGATCAT GGCGGTGAGT TGTACATTCA GAAAGCCTCC 
CAGCTGACGC ACCTGTTACT CCTGCTAACT GCCTACGCCA AGAGGGAACT ACAGCCTCGG 
GAAAGCCTTC ACATGCTGGA GAAAATTGGG CTTTATAGTC GGCGCCTCCA GTTCCGTGTC 
GTCAATGGTA GTCAAAACAC ACAGAATTGT GCACCTCTAC AATTGTATGT GGATTTCCTA 
TTGACTTTGG TTAAGAACAC CAAATGGACA GCTCTAGCAT CCACTCCTTG GAATCAGATG 
ACCGATGAGC TTCGCCTCTG TCTGCGCCTG CTGGAAATAA TATGCGCCCA AGTGTTTTCG 
GAAAAAGCTG ATCAGCCAGA GCGTCAGGAG TGGACACGAG CCCTGCAGCA AAGTTTGCAA 
CTAATCCTGC CTGAAGCTCA AGACAGATTG GAAGTGCTAT CAAACTTCTA TGTGTTCGAG 
CGACTGCCAG AATTGTGGCC TCGAGACTCT GACTATGCCG TGTTCCGGTT GCAAGGGTTT 
ATTATTCTAG AAGCGGTGCT GTCCAATCCG AAATCCCAAA TAGACTGCGG TCTGGTTCAT 
GTGCTGCGGG TGGCCAACGC CTGTGGATCT CCCTTACAAA CTCTGCGAGT GCAGGCCATT 
AACATTCTGC AATTAATCAG TAATCGCAAG CTGGTGAGCC ATGTGGAGCA ATTGGTGCGT 
TCGCTGCTGC AGCGCAAGAG TGAGTTGAGC ATGGATCATG AACAATATGC CCTGATTCTA 
TACACCATTC TGGAACCAGA GAAGGCAACT GCCAAGGAAA GACTCGTGCT GTCAAAACTG 
AAACGATCTG TTTTGGCTTT GGCCTCCGAT CCGAAGCAGT CTCCCATTTG TACGGCGTCT 
TTGCTTGCGG CCCTAAAGCA CGTCAACGAT GAGAATTTCT TAAACGAATT GCTGCCGCTG 
GGTCTTGACT CCCTGAAGAC GATAACTGCG GGAGAAGACA ACCAGAATAT AAAACAGCTG 
CCATGGCCAC ATAGCGAAAT CTACAAGTCG GTGATAGAAA GGTTTGAGGG GCGTGTTGCG 
CTGAATGTAT TGCTGCGAAA GGATCTGGCT TGGAAATTGT TTGAAGACAG CTTCGCCCAG 
TATGACACCT ATGTCCAACT TGAACAGAAG CTACAGCCTC TTCCCTGCGT CCTGCTAAAC 
AGCCTCACGC CGGAGACCTT TGAGCAAATG CATGCCAAGC ACAAGATTGC TCTAATCAAG 
CTGATAGTGG AGTCGGCTAC CAATTCGGAC AATGACAGCA TATTCTTGGC TAGCCATCGT 
TTGCTGAAGC GCTGCCGTTT GGATTGCCAG CCACTCGTAC CGATCCTGTT GGAGATGGCC 
AACACCAAGG TGGAGAAGAA ACAACCCGTC AAACGACGAT CAGTACAGGC AACCCAACTG 
GATCTAACCA GCCCATACTG GAAGCAGGGA ATGACCCTTC TCGAGCTGTT GGAGCACAAG 
AAACAGCTGG TTGGAGCGGA GCTGCTGATC CCACCGCTCT TCGAACTTCT ACAGGCCTGT 
CTTACGATGG AAGAGCATAG TGCCGCCGAG TATCCCAAGC AGCTGATTCT CTCAAGCTTG 
CTGCACTGCT GTCAGACGGC ACAGTCGGCC GGTGTGCAGC TCGTTAAGGC TATGCCTGAA 
TCTAGCTTCC GCATCGAGCT GGTGGTGCAA TCCTTGAGGA ATACCCGAAA TCCGCAGACC 
CAGCAGCATG CGCTGCTTTT CCTGACTCAC TGTGCTGGCA TGTATCCGCA ACAAGTTCTA 
CACAAGATCG TTGAAATCTT TACGTTCGTT GGATCCACGG TGGCGCGACA CGACGATGCC 
TTCAGCCTGC ATATAATTCA CAATGTGGTG GAGTCGATTA TACCCATTCT GCTTCTGAAT 
ACCGGACATA ATGAGCTGGT TATACCCGTG CTTAAGGTGT TCGCTGATAT TTGCACGGAC 
GTGCCGGTCC ATCGGAGATT GCCATTGTAT GCTACCCTGT TCCGAGTTCT GGAGCCCAAG 
GAGCACCTTT GGCAGTTCTT GTGCATTATC TTTGAGTCTC AAGTGCTTCT GGAGCAAGTT 
CCACAAAAAG TGTCCACAGA CAAGTCGCGG CTGGACTTTG CCCGTGAACT GACGTTGATG 
TTCGAAGATC CGACGGTCGC AATTCAGACG TGCATCCGTC TATTGGATTA CTTGGCCAAG 
TTGCCGGCAA CCAAAAGCAG TCTTTCGGGT GGATCAGGCT CCTCTGTGCT AAGCACGGAA 
CAACAGCTCT TTGATGTTCG GACACGGACG TTCAAGCAAC TGCGGCACTA CAAGTACCTC 
ATCATGGACT TCCTTTCTGG AATTAGCAGC TGCAACGAAT GGGAGAAGAA GATGAAGCGT 
CCTGATCCCA ACGAGCTACT TCCGTATTAT CAGGAATTCA TCCTAAAGAC ACTCGCGTAT 
GTGGGCGTAC TTAATGGAGC ATTGGAAGCA GCCTCCGAAA CGCCTTCTCT GGAAAAGTTC 
TGGCGCGTGC TAGCTAACCA TGCACACGAT GTCTTGGATA ACGCCATCGG TCTTTTGGCA 
CCGCAACATT TCATCAGTGT GATAACCGAG CTACTGAAGC ATGATCATGT GTATGTGCGC 
ATTAAGGTGA TGGACTTGTT GGTGACCAAG CTAAGTCCGT CGAGCGATTA CTTCCAGCAA 
TCCAACGCTG AACACTTTGG AGTGCTTTTC GCTCCTTTGC AAGAGATTAT CAACGGAATC 
CTCGAGGGCA GTTCCAACAG TGCGCAGCAG GCGAAGCTTC AACAGACTGC GTTGCATGCC 
CTACAGTTGT TGGCCCTCCG TCACGGACGC GACTACATCG AGGAGTGTCG CTCTCTTCTG 
GCCACCTTGA CCAAGATCAC CAAACGACGT GCGAACGTGC CCAAGGCGGT AGTGGGCAAT 
GTGGTGCTGA CCCTCGTCGA AATTTGCGCA AGTCTTAAGG CCCATGCCTT GGCCCAATTG 
CCCAAGTTCG CTCCCCAACT TACTGAGTTA CTGAAGGAAC AGGTTCACCA AATGGCCTCC 
CTCAAACAAG GACCAGATTA CGTCTGTTCC ACCCTTGTCA CAGCACTCCA CAAGCTCTTC 
AAGGCGTTGC CGCTTTTCTT GGGACCCTAC TTGGTGGACA TTATCGGAGG CTTGGCGCGC 
CTTTCAGTGC AATTGGAAAA TCCACAGCTG CTGCAGGATA AGCGCACACA GGTGCTTAAG 
CAGAAACTTG CGGACGTTTG GAGTGCAGTT GCCCAGGGCG TCGAGGTGCG CATTCTTGTG 
CCCAGTTGCG CCAAGGCGTT CTCCAGTCTA TTAGAGCAGC AAGCGTACGA CGAGCTAGGA 
CATCTAATGC AGCAGCTGTT GCTGCAGAGC GTCCGACACA ATTCAGCCGC TCAATTGCAA 
CCGGTTCAGG ATCCCCTAAG CGAGCTCTTC CTGCAAGCAC TCAACTTCCG GCTGCAAGTG 
CGTGGACTTG GTTTGCAGAG GCAATTGGTT TCGGATGTAG AGGCTTCCAT AACCGAGACC 
TTTGTGACGT GGATCCTAAA GTTGTCAGAG ACCAGTTTCC GTCCCATGTA CTCTCGGGTA 
CATAAGTGGG CACTGGAGAG TACCTCTCGG GAAACGCGTC TCACCTACTT CCTGCTGACC 
AACCGGATCG CCGAAGCTCT AAAATCTTTG TTCGTGCTAT TTGCCAGCGA TTTCGTGGAA 
GACTCTTCGC GCTTGCTGAC GGAGCACAAC TCAATCCGTC CCGAGTTCGA AGTCGAAGAA 
AGGGAAGATG ATGTGGACCT GTTAATGGCA ATACTGAACA CTCTGCATCA TGTGTTCTTG 
TACTGCAGCG AGGACTTCAT TAATGACCAC CGCTTCAACG TGTTAATGCC ACCGCTGGTC 
AACCAATTAG AAAACGACCT GGTATTGGGA AATGAGTCGC TTCAGCAAGT TCTCAGTAAC 
TGCATTGCCC AGTTTGCGGT GGCCACCAAC GATGTGATGT GGAAGCAGCT CAACAGCCAA 
GTGCTCCTAA AGACACGCAC ATCCAATCCC GAAGTGCGGA TTCTGGCATT CAACAGTTGT 
GTGGCTATCG CGCGCAAACT GGGTGAAAGC TATGCCGCCC TGTTGCCAGA GACAGTTCCA 
TTCATCGCTG AGCTCCTGGA GGACGAGCAT CAACGTGTGG AGAAGAACAC CCGGACTGGA 
GTGCAGGAGC TAGAAACTAT TTTAGGCGAG TCTGTGCAAA AGTATCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(2)k09022 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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