chic cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	chic
Entrez Gene ID	33834
Full Name	chickadee
Synonyms	CG9553,Chi,Chic,D88,Dmel\CG9553,GH4,chi,l(2)27/7,profilin,sand
General protein information	Preferred Names chickadee Names CG9553 gene product from transcript CG9553-RD CG9553-PA CG9553-PB CG9553-PC CG9553-PD CG9553-PE CG9553-PF DProfilin chic-PA chic-PB chic-PC chic-PD chic-PE chic-PF chicadee chickaddee profilin stranded
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26A5-26B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057668.6	NP_477016.1	chickadee, isoform A
NM_164667.2	NP_723136.1	chickadee, isoform C
NM_205913.3	NP_995635.1	chickadee, isoform D
NM_001258976.2	NP_001245905.1	chickadee, isoform E
NM_134304.3	NP_599131.1	chickadee, isoform B
NM_001258977.2	NP_001245906.1	chickadee, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009861	XP_553744.3
Kluyveromyces lactis	KLLA0E02289g	XP_454051.1
Schizosaccharomyces pombe (fission yeast)	cdc3	NP_593827.1
Saccharomyces cerevisiae (baker's yeast)	PFY1	NP_014765.3
Arabidopsis thaliana (thale cress)	PRF4	NP_194663.1
Arabidopsis thaliana (thale cress)	PRF5	NP_179567.1
Drosophila melanogaster (fruit fly)	chic	NP_001245905.1
Neurospora crassa	NCU06397	XP_962235.1
Oryza sativa (rice)	Os06g0152100	NP_001056833.1
Oryza sativa (rice)	Os10g0323900	NP_001064349.1
Caenorhabditis elegans (roundworm)	pfn-3	NP_508205.1
Eremothecium gossypii	AGOS_ACL168C	NP_983236.1
Magnaporthe oryzae (rice blast fungus)	MGG_06127	XP_003711995.1
Oryza sativa (rice)	Os10g0323600	NP_001064348.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chic regulates interactions across the somatic permeability barrier around the germline that is essential for Drosophila spermatogenesis.
Title: A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.

chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain germline stem cells, possibly facilitating localization or maintenance of E-cadherin to the germline stem cell-hub cell interface.
Title: The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.

Data show that chic-deficient larvae fail to form a robust actin cable along the wound edge and also fail to form normal filopodial and lamellipodial extensions into the wound gap.
Title: Transcriptional regulation of Profilin during wound closure in Drosophila larvae.

D. melanogaster DAAM formin represents an extreme class of barbed end regulators gated by profilin.
Title: Characterization of the biochemical properties and biological function of the formin homology domains of Drosophila DAAM.

FMRP1 binds the mRNA of the Drosophila profilin and negatively regulates Profilin protein expression
Title: The Drosophila fragile X mental retardation protein controls actin dynamics by directly regulating profilin in the brain.

The following chic gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chic cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13850	NM_134304.3 Latest version!	Drosophila melanogaster chickadee (chic), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13850	NM_164667.2 Latest version!	Drosophila melanogaster chickadee (chic), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13850	NM_001258977.2 Latest version!	Drosophila melanogaster chickadee (chic), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13850	NM_001258976.2 Latest version!	Drosophila melanogaster chickadee (chic), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13850	NM_057668.6 Latest version!	Drosophila melanogaster chickadee (chic), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13850	NM_205913.3 Latest version!	Drosophila melanogaster chickadee (chic), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chic ( NM_057668.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477016.1 Drosophila melanogaster chickadee (chic), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13850
Clone ID Related Accession (Same CDS sequence)	NM_057668.6 , NM_164667.2 , NM_205913.3 , NM_001258976.2 , NM_134304.3 , NM_001258977.2
Accession Version	NM_057668.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chickadee, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057668.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477016.1
CDS	251..631
Translation	MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY

Target ORF information:

RefSeq Version	NM_057668.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chickadee (chic), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057668.6

ORF Insert Sequence:

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chic ( NM_164667.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723136.1 Drosophila melanogaster chickadee (chic), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13850
Clone ID Related Accession (Same CDS sequence)	NM_057668.6 , NM_164667.2 , NM_205913.3 , NM_001258976.2 , NM_134304.3 , NM_001258977.2
Accession Version	NM_164667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chickadee, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Mar 18, 2004 this sequence version replaced NM_164667.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723136.1
CDS	456..836
Translation	MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY

Target ORF information:

RefSeq Version	NM_164667.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chickadee (chic), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164667.2

ORF Insert Sequence:

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chic ( NM_205913.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995635.1 Drosophila melanogaster chickadee (chic), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13850
Clone ID Related Accession (Same CDS sequence)	NM_057668.6 , NM_164667.2 , NM_205913.3 , NM_001258976.2 , NM_134304.3 , NM_001258977.2
Accession Version	NM_205913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chickadee, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205913.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995635.1
CDS	249..629
Translation	MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY

Target ORF information:

RefSeq Version	NM_205913.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chickadee (chic), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205913.3

ORF Insert Sequence:

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chic ( NM_001258976.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245905.1 Drosophila melanogaster chickadee (chic), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa13850
Clone ID Related Accession (Same CDS sequence)	NM_057668.6 , NM_164667.2 , NM_205913.3 , NM_001258976.2 , NM_134304.3 , NM_001258977.2
Accession Version	NM_001258976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chickadee, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258976.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245905.1
CDS	355..735
Translation	MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY

Target ORF information:

RefSeq Version	NM_001258976.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chickadee (chic), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258976.2

ORF Insert Sequence:

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chic ( NM_134304.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_599131.1 Drosophila melanogaster chickadee (chic), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13850
Clone ID Related Accession (Same CDS sequence)	NM_057668.6 , NM_164667.2 , NM_205913.3 , NM_001258976.2 , NM_134304.3 , NM_001258977.2
Accession Version	NM_134304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chickadee, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134304.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599131.1
CDS	345..725
Translation	MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY

Target ORF information:

RefSeq Version	NM_134304.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chickadee (chic), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134304.3

ORF Insert Sequence:

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chic ( NM_001258977.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245906.1 Drosophila melanogaster chickadee (chic), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa13850
Clone ID Related Accession (Same CDS sequence)	NM_057668.6 , NM_164667.2 , NM_205913.3 , NM_001258976.2 , NM_134304.3 , NM_001258977.2
Accession Version	NM_001258977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chickadee, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258977.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245906.1
CDS	325..705
Translation	MSWQDYVDNQLLASQCVTKACIAGHDGNIWAQSSGFEVTKEELSKLISGFDQQDGLTSNGVTLAGQRYIYLSGTDRVVRAKLGRSGVHCMKTTQAVIVSIYEDPVQPQQAASVVEKLGDYLITCGY

Target ORF information:

RefSeq Version	NM_001258977.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chickadee (chic), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258977.2

ORF Insert Sequence:

ATGAGCTGGC AAGATTATGT GGACAACCAA CTCCTGGCCT CGCAGTGCGT GACCAAGGCG 
TGCATCGCCG GCCACGACGG CAACATTTGG GCGCAGTCCA GTGGCTTTGA GGTGACAAAA 
GAGGAGCTCT CCAAACTGAT CAGCGGCTTT GACCAGCAGG ACGGTCTCAC CAGCAACGGC 
GTGACACTCG CCGGCCAGCG GTACATTTAC CTTTCCGGCA CAGACCGCGT GGTGCGCGCC 
AAGCTCGGCC GGAGCGGAGT GCACTGCATG AAGACAACAC AAGCCGTGAT CGTTTCCATC 
TACGAGGATC CCGTTCAGCC CCAGCAGGCC GCTTCCGTGG TAGAGAAACT TGGAGATTAT 
CTGATTACTT GCGGGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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chic cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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