Chc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chc
Entrez Gene ID	32537
Full Name	Clathrin heavy chain
Synonyms	CG9012,CHC,CLH,Cla,Clh,D-Chc,Dmel\CG9012,chc,chc1,dCHC,l(1)13Fb,l(1)G0438,l(1)VI
General protein information	Preferred Names Clathrin heavy chain Names CG9012 gene product from transcript CG9012-RF CG9012-PA CG9012-PB CG9012-PC CG9012-PD CG9012-PE CG9012-PF CG9012-PG Chc-PA Chc-PB Chc-PC Chc-PD Chc-PE Chc-PF Chc-PG clathrin clathrin hc clathrin heavy chain clathrin heavy-chain
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13F5-13F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103524.2	NP_001096994.1	clathrin heavy chain, isoform F
NM_057694.3	NP_477042.1	clathrin heavy chain, isoform A
NM_206728.2	NP_996451.1	clathrin heavy chain, isoform D
NM_001103523.2	NP_001096993.1	clathrin heavy chain, isoform E
NM_001298371.1	NP_001285300.1	clathrin heavy chain, isoform G
NM_206729.2	NP_996452.1	clathrin heavy chain, isoform C
NM_167466.2	NP_727901.1	clathrin heavy chain, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bic-D is also required to transport Chc mRNA into the oocyte and for transport and proper localization of Chc protein to the oocyte cortex.
Title: Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.

Results report a Drosophila disabled (DAB)mutant revealing a novel role for DAB proteins in chemical synaptic transmission, and support a role for clathrin-mediated endocytosis in rapid clearance of neurotransmitter release sites.
Title: The DISABLED protein functions in CLATHRIN-mediated synaptic vesicle endocytosis and exoendocytic coupling at the active zone.

Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling.
Title: Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling.

chc mRNA and protein are highly expressed from early stages of embryogenesis onwards, consistent with genetic studies predicting a maternal contribution of the gene function
Title: Expression and localization of clathrin heavy chain in Drosophila melanogaster.

Chc is critical for synaptic vesicle recycling and in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization.
Title: Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake.

The following Chc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03614	NM_206729.2 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant C (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa03614	NM_001103524.2 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant F (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa03614	NM_001298371.1 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant G (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa03614	NM_167466.2 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant B (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa03614	NM_057694.3 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant A (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa03614	NM_001103523.2 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant E (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa03614	NM_206728.2 Latest version!	Drosophila melanogaster clathrin heavy chain, transcript variant D (Chc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chc ( NM_001103524.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096994.1 Drosophila melanogaster clathrin heavy chain, transcript variant F (Chc), mRNA.

Price & Availability↑

CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_001103524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103524.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096994.1
CDS	255..5291
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_001103524.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant F (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103524.2

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chc ( NM_057694.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477042.1 Drosophila melanogaster clathrin heavy chain, transcript variant A (Chc), mRNA.

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CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_057694.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057694.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477042.1
CDS	374..5410
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_057694.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant A (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057694.3

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chc ( NM_206728.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996451.1 Drosophila melanogaster clathrin heavy chain, transcript variant D (Chc), mRNA.

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CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_206728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206728.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996451.1
CDS	471..5507
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_206728.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant D (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206728.2

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chc ( NM_001103523.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096993.1 Drosophila melanogaster clathrin heavy chain, transcript variant E (Chc), mRNA.

Price & Availability↑

CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_001103523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103523.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096993.1
CDS	361..5397
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_001103523.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant E (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103523.2

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chc ( NM_001298371.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285300.1 Drosophila melanogaster clathrin heavy chain, transcript variant G (Chc), mRNA.

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CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_001298371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285300.1
CDS	466..5502
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_001298371.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant G (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298371.1

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chc ( NM_206729.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996452.1 Drosophila melanogaster clathrin heavy chain, transcript variant C (Chc), mRNA.

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CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_206729.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206729.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996452.1
CDS	412..5448
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_206729.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant C (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206729.2

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chc ( NM_167466.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727901.1 Drosophila melanogaster clathrin heavy chain, transcript variant B (Chc), mRNA.

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CloneID	OFa03614
Clone ID Related Accession (Same CDS sequence)	NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
Accession Version	NM_167466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clathrin heavy chain, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167466.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727901.1
CDS	331..5367
Translation	MTQPLPIRFQEHLQLTNVGINANSFSFSTLTMESDKFICVREKVNDTAQVVIIDMNDATNPTRRPISADSAIMNPASKVIALKAQKTLQIFNIEMKSKMKAHTMNEDVVFWKWISLNTLALVTETSVFHWSMEGDSMPQKMFDRHSSLNGCQIINYRCNASQQWLLLVGISALPSRVAGAMQLYSVERKVSQAIEGHAASFATFKIDANKEPTTLFCFAVRTATGGKLHIIEVGAPPNGNQPFAKKAVDVFFPPEAQNDFPVAMQVSAKYDTIYLITKYGYIHLYDMETATCIYMNRISADTIFVTAPHEASGGIIGVNRKGQVLSVTVDEEQIIPYINTVLQNPDLALRMAVRNNLAGAEDLFVRKFNKLFTAGQYAEAAKVAALAPKAILRTPQTIQRFQQVQTPAGSTTPPLLQYFGILLDQGKLNKFESLELCRPVLLQGKKQLCEKWLKEEKLECSEELGDLVKASDLTLALSIYLRANVPNKVIQCFAETGQFQKIVLYAKKVNYTPDYVFLLRSVMRSNPEQGAGFASMLVAEEEPLADINQIVDIFMEHSMVQQCTAFLLDALKHNRPAEGALQTRLLEMNLMSAPQVADAILGNAMFTHYDRAHIAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHMLNAEWLVSFFGTLSVEDSLECLKAMLTANLRQNLQICVQIATKYHEQLTNKALIDLFEGFKSYDGLFYFLSSIVNFSQDPEVHFKYIQAACKTNQIKEVERICRESNCYNPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDIIKNLILVVKGQFSTDELVEEVEKRNRLKLLLPWLESRVHEGCVEPATHNALAKIYIDSNNNPERYLKENQYYDSRVVGRYCEKRDPHLACVAYERGLCDRELIAVCNENSLFKSEARYLVGRRDAELWAEVLSESNPYKRQLIDQVVQTALSETQDPDDISVTVKAFMTADLPNELIELLEKIILDSSVFSDHRNLQNLLILTAIKADRTRVMDYINRLENYDAPDIANIAISNQLYEEAFAIFKKFDVNTSAIQVLIDQVNNLERANEFAERCNEPAVWSQLAKAQLQQGLVKEAIDSYIKADDPSAYVDVVDVASKVESWDDLVRYLQMARKKARESYIESELIYAYARTGRLADLEEFISGPNHADIQKIGNRCFSDGMYDAAKLLYNNVSNFARLAITLVYLKEFQGAVDSARKANSTRTWKEVCFACVDAEEFRLAQMCGLHIVVHADELEDLINYYQNRGYFDELIALLESALGLERAHMGMFTELAILYSKFKPSKMREHLELFWSRVNIPKVLRAAESAHLWSELVFLYDKYEEYDNAVLAMMAHPTEAWREGHFKDIITKVANIELYYKAIEFYLDFKPLLLNDMLLVLAPRMDHTRAVSYFSKTGYLPLVKPYLRSVQSLNNKAINEALNGLLIDEEDYQGLRNSIDGFDNFDNIALAQKLEKHELTEFRRIAAYLYKGNNRWKQSVELCKKDKLYKDAMEYAAESCKQDIAEELLGWFLERDAYDCFAACLYQCYDLLRPDVILELAWKHKIVDFAMPYLIQVLREYTTKVDKLELNEAQREKEDDSTEHKNIIQMEPQLMITAGPAMGIPPQYAQNYPPGAATVTAAGGRNMGYPYL

Target ORF information:

RefSeq Version	NM_167466.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clathrin heavy chain, transcript variant B (Chc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167466.2

ORF Insert Sequence:

ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG 
CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT 
CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT 
GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG 
GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT 
CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG 
ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC 
TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC 
ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT 
TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT 
CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT 
CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC 
CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA 
TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC 
GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC 
AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC 
GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC 
GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG 
GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT 
TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC 
ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC 
TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC 
AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT 
CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG 
AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC 
TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG 
GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG 
CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC 
CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC 
CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG 
GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC 
GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG 
GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG 
CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT 
GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC 
AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT 
CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG 
AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT 
CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC 
ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT 
CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA 
TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT 
CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG 
GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC 
TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC 
GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG 
TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC 
CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG 
TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT 
TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC 
GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA 
GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG 
ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA 
TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC 
GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT 
CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT 
GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG 
GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC 
ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG 
AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC 
AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG 
GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC 
TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT 
GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG 
ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA 
TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC 
TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG 
GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG 
GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC 
AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG 
AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC 
TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT 
AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT 
CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC 
GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT 
CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG 
TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA 
CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT 
GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG 
ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG 
CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG 
ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT 
GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Chc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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