solo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	solo
Entrez Gene ID	26067079
Full Name	sisters on the loose
Synonyms	BG:DS00929.15,CG33678,CG43081,CG46282,Dmel\CG46282,SOLO,cgt,fs(2)ltoRJ36,pf7
General protein information	Preferred Names sisters on the loose Names CG46282 gene product from transcript CG46282-RA CG46282-PA CG46282-PB courgette female sterile(2)ltoRJ36 no-relish solo-PA solo-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35B10-35C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032103.2	NP_001027274.2	sisters on the loose, isoform A
NM_001316394.1	NP_001303323.1	sisters on the loose, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following solo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the solo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15891	NM_001316394.1 Latest version!	Drosophila melanogaster sisters on the loose (solo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa15891	NM_001032103.2 Latest version!	Drosophila melanogaster sisters on the loose (solo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

solo ( NM_001032103.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027274.2 Drosophila melanogaster sisters on the loose (solo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15891
Clone ID Related Accession (Same CDS sequence)	NM_001032103.2 , NM_001316394.1
Accession Version	NM_001032103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sisters on the loose, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_001032103.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGCTAAGGAA 
CTGCCGCCGA TCCCATCTAG CTGCGATAAG GATGAGGTGG GCGCATTCAT CGATCAGCTG 
GACTTGAGCA AGTACACGAC CAGCCTAGCA CTGATTCGAA AATTGCGTGA CTACTTGCTG 
ACTGCATTTA AGGCTTTGGT GAACCAAGCA TGGGCTTCTC TCGAGAAATT AGCTCAAGAA 
AAGCGTGCAC AAAAGGCACA TCAGATACGC GAAACAACAG ATATGATGAA TCGCCTTATG 
GAGGACATGG GCCGATTGAT TCGTGAAGGT GAACATGAGG AAGCAAATGG GATTGGGATG 
GGATTTCAAA GCATTGATGC GGATTGCAGA GCTGATTTGT CGCAATGGAG GAATTTACAG 
CAAGTTGTGA TGCCGCAATC TTCGGAGATC AGAGATGGAC AAACAAACAG GACAAGCGGT 
ACTGAGGGCG GTCGATCAGG AGGTTTAACC GATGGCGATG ACTTAACCGA AATACAAAGC 
GCCGCATACG CCGTGTATCA TCAGGTTTTG GATCGAGGTT ACCAAACGGG TCGACGGCTG 
TATGATGATA ATCAAGGAGC ATTGAGAGAA GATTCTATTC CAACAGAAGA CCGAGAACAC 
CCAGAAAATA ATCAAGTGGA TGGATCAACA TATGGAGTGA CTAGATTTCT GCACTGGCTA 
AGTGAGAACT TTGTCACTCG GCATGAAATA AGGGCCGTAC GTTTTGGCAG TCCGCATATG 
GTTTTTCAAT CTTATGACTG GCCAGAGTCT CCAAAGCAGC ACATTTCGAC GGTCCATCAA 
AAAGGCATAT TTTATTCTCA GCCCTTAATT AGGACACGCA TAATAGCCAC TGTTGCGAGC 
GAGCCAAACA ATTCGAGTAG TGGTCAGCTC AGCAGTATGG TAAACTTGGC ACGACATCAG 
CGGTCGCAAA ATCAGGAAAC GCCAGATGGA ATTTACGTTA GTCGCTACAA TGCAACTGGA 
GATGAGGAGA CTCCCTGCTC CGCAACCGGA GTCCCCGGCA GAGTTGTGGC TCTGCGTGCT 
TCGAGCACCC CAAATGAACA TAGGAGCGAT CCAGGCTTGT CTTTTATACC CAATTTGACT 
TACGAAGATG GGACCATACC ATCATTGGCA GCAGGCGGCG CTGCATCGAC ACCGTTGGCT 
CCATCACTGC CAGCAGAGCA TTCATTGGGG TTAGAAATGG ATAGCAACAC TTTGGTGGTA 
CCAGCTGATG CGGATGAAAC CCGCTTAGTC GGACATCCAG GATCAACAGT GGTTCGTTCT 
CGGCTTGGAG GCCCTCCAGC ATCAACACCT TTACTATCAT TCCGACCTAG ACTGCCGGTC 
ATTGACGAAG AGCATCATCT ACGCTTTACC AGGGTGTCAC GCGAGCTGCA AATGAGTGTT 
GCTTTCCCGC CGGAAGAGAC AACACTAAGC GAGCAACGTG AGTGGGGCTC GATTTTCCGA 
CCACGTGATG AAATGGTTAA CTATGCCGAA GGTCCACCTC CTCGCCAAAC ACAAAGGGCG 
CGTCGTCGCT CGCGTACACG CAGACGACGT AATCGTATCG CTTCAGCTGC CAGCCCGCAA 
CTAACACCGG AACGAATTGT CCGTCGCGAG GAAGCCTTCC AAAGCAGCCG ACATATTGCA 
GCCACTTTTA TGTCCAGCTT GCTACAAAAT GCAGCTGGAG AAGTGACGAC GTCTTCAGGT 
CTGGCAGTAA CCCGTTCAGT GAAAGAAATA ACGACATCAG TCCATATCAC CCGAAATGTA 
CTACAAGAGG AGGTACCACC GACCACAGAA GCAACGACGA CCTCCCTAGA AGTTGCACAG 
GCAACAGGGT ATTCGGATTC TGGGGCTTGT ATGCAATCTG CAGCGTTCTC TCAAGATCTT 
CCTGTAGTAG CTCCCTTGGA GATATCTGGA AATGCATCAA GTGACCAAGC ATCATTTGAT 
CAAGCTTTGG CCTTACTGCC TCCCATAAAT TCCACTCCAA TAGGTCAGCA TCCAATTGCT 
GTTTTCGGAC CTAAAACCGA AATTGAAATT AAACCCCAAA AAATGGACAT CTCTAATATG 
ATGAATACAT CGGTTCCACC AATGCCAATG GAAGTCGACG AAGTTCCACT GGAGGTATCA 
AACACTTTGG CCAATATGAG CGATCACGCT TATGTGACTG ATATACAGAT ACTACCGTCG 
TTGCAACTCA ATTCTTTGAA TCTTGTTAGC ACCAATATTA CAGACAATGC AGTAAATATC 
GCCCCATCGA CATCAACAAA TGATTTGCAA ACGCCAAGTG ACCGCAGAAC ATCAATGCAA 
AATCAACTAT ATCGAGTGTC CATGAACGAT TTGGCATATA TTTCACAACA CGCTAATGTG 
GTTCGTCTGA TGGTAGATCG TCAAGAGGAA CTAGGGGCAC AGGTCAGCTC CCTGGGGCCT 
TTTAAGGAAA ATAGCCAAGG CGCGCTAAAT TTTAGTACAA TGCCCGCTGT CAAAGTCTAC 
GATCCACGTA TAATTCAGAA CATTCAGAAG GATAAGATGA GAGTAGTTCA TGGGCTCTTC 
GGTGCTCTTA TCAGACAGTC CCAGGTTGAT ATGCACAATG CACCATTCAT CCGAAATCGC 
AAGGATGCGT TGATGGCATA TCGCTTTTTA CTCGAGCTTA AGACTGCAAA TATTATAAGT 
CTTAGCTCGG ATGGTAGATT TTTCGGGCTG CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027274.2
CDS	152..3247
Translation	MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDAKELPPIPSSCDKDEVGAFIDQLDLSKYTTSLALIRKLRDYLLTAFKALVNQAWASLEKLAQEKRAQKAHQIRETTDMMNRLMEDMGRLIREGEHEEANGIGMGFQSIDADCRADLSQWRNLQQVVMPQSSEIRDGQTNRTSGTEGGRSGGLTDGDDLTEIQSAAYAVYHQVLDRGYQTGRRLYDDNQGALREDSIPTEDREHPENNQVDGSTYGVTRFLHWLSENFVTRHEIRAVRFGSPHMVFQSYDWPESPKQHISTVHQKGIFYSQPLIRTRIIATVASEPNNSSSGQLSSMVNLARHQRSQNQETPDGIYVSRYNATGDEETPCSATGVPGRVVALRASSTPNEHRSDPGLSFIPNLTYEDGTIPSLAAGGAASTPLAPSLPAEHSLGLEMDSNTLVVPADADETRLVGHPGSTVVRSRLGGPPASTPLLSFRPRLPVIDEEHHLRFTRVSRELQMSVAFPPEETTLSEQREWGSIFRPRDEMVNYAEGPPPRQTQRARRRSRTRRRRNRIASAASPQLTPERIVRREEAFQSSRHIAATFMSSLLQNAAGEVTTSSGLAVTRSVKEITTSVHITRNVLQEEVPPTTEATTTSLEVAQATGYSDSGACMQSAAFSQDLPVVAPLEISGNASSDQASFDQALALLPPINSTPIGQHPIAVFGPKTEIEIKPQKMDISNMMNTSVPPMPMEVDEVPLEVSNTLANMSDHAYVTDIQILPSLQLNSLNLVSTNITDNAVNIAPSTSTNDLQTPSDRRTSMQNQLYRVSMNDLAYISQHANVVRLMVDRQEELGAQVSSLGPFKENSQGALNFSTMPAVKVYDPRIIQNIQKDKMRVVHGLFGALIRQSQVDMHNAPFIRNRKDALMAYRFLLELKTANIISLSSDGRFFGLL

Target ORF information:

RefSeq Version	NM_001032103.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sisters on the loose (solo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032103.2

ORF Insert Sequence:

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGCTAAGGAA 
CTGCCGCCGA TCCCATCTAG CTGCGATAAG GATGAGGTGG GCGCATTCAT CGATCAGCTG 
GACTTGAGCA AGTACACGAC CAGCCTAGCA CTGATTCGAA AATTGCGTGA CTACTTGCTG 
ACTGCATTTA AGGCTTTGGT GAACCAAGCA TGGGCTTCTC TCGAGAAATT AGCTCAAGAA 
AAGCGTGCAC AAAAGGCACA TCAGATACGC GAAACAACAG ATATGATGAA TCGCCTTATG 
GAGGACATGG GCCGATTGAT TCGTGAAGGT GAACATGAGG AAGCAAATGG GATTGGGATG 
GGATTTCAAA GCATTGATGC GGATTGCAGA GCTGATTTGT CGCAATGGAG GAATTTACAG 
CAAGTTGTGA TGCCGCAATC TTCGGAGATC AGAGATGGAC AAACAAACAG GACAAGCGGT 
ACTGAGGGCG GTCGATCAGG AGGTTTAACC GATGGCGATG ACTTAACCGA AATACAAAGC 
GCCGCATACG CCGTGTATCA TCAGGTTTTG GATCGAGGTT ACCAAACGGG TCGACGGCTG 
TATGATGATA ATCAAGGAGC ATTGAGAGAA GATTCTATTC CAACAGAAGA CCGAGAACAC 
CCAGAAAATA ATCAAGTGGA TGGATCAACA TATGGAGTGA CTAGATTTCT GCACTGGCTA 
AGTGAGAACT TTGTCACTCG GCATGAAATA AGGGCCGTAC GTTTTGGCAG TCCGCATATG 
GTTTTTCAAT CTTATGACTG GCCAGAGTCT CCAAAGCAGC ACATTTCGAC GGTCCATCAA 
AAAGGCATAT TTTATTCTCA GCCCTTAATT AGGACACGCA TAATAGCCAC TGTTGCGAGC 
GAGCCAAACA ATTCGAGTAG TGGTCAGCTC AGCAGTATGG TAAACTTGGC ACGACATCAG 
CGGTCGCAAA ATCAGGAAAC GCCAGATGGA ATTTACGTTA GTCGCTACAA TGCAACTGGA 
GATGAGGAGA CTCCCTGCTC CGCAACCGGA GTCCCCGGCA GAGTTGTGGC TCTGCGTGCT 
TCGAGCACCC CAAATGAACA TAGGAGCGAT CCAGGCTTGT CTTTTATACC CAATTTGACT 
TACGAAGATG GGACCATACC ATCATTGGCA GCAGGCGGCG CTGCATCGAC ACCGTTGGCT 
CCATCACTGC CAGCAGAGCA TTCATTGGGG TTAGAAATGG ATAGCAACAC TTTGGTGGTA 
CCAGCTGATG CGGATGAAAC CCGCTTAGTC GGACATCCAG GATCAACAGT GGTTCGTTCT 
CGGCTTGGAG GCCCTCCAGC ATCAACACCT TTACTATCAT TCCGACCTAG ACTGCCGGTC 
ATTGACGAAG AGCATCATCT ACGCTTTACC AGGGTGTCAC GCGAGCTGCA AATGAGTGTT 
GCTTTCCCGC CGGAAGAGAC AACACTAAGC GAGCAACGTG AGTGGGGCTC GATTTTCCGA 
CCACGTGATG AAATGGTTAA CTATGCCGAA GGTCCACCTC CTCGCCAAAC ACAAAGGGCG 
CGTCGTCGCT CGCGTACACG CAGACGACGT AATCGTATCG CTTCAGCTGC CAGCCCGCAA 
CTAACACCGG AACGAATTGT CCGTCGCGAG GAAGCCTTCC AAAGCAGCCG ACATATTGCA 
GCCACTTTTA TGTCCAGCTT GCTACAAAAT GCAGCTGGAG AAGTGACGAC GTCTTCAGGT 
CTGGCAGTAA CCCGTTCAGT GAAAGAAATA ACGACATCAG TCCATATCAC CCGAAATGTA 
CTACAAGAGG AGGTACCACC GACCACAGAA GCAACGACGA CCTCCCTAGA AGTTGCACAG 
GCAACAGGGT ATTCGGATTC TGGGGCTTGT ATGCAATCTG CAGCGTTCTC TCAAGATCTT 
CCTGTAGTAG CTCCCTTGGA GATATCTGGA AATGCATCAA GTGACCAAGC ATCATTTGAT 
CAAGCTTTGG CCTTACTGCC TCCCATAAAT TCCACTCCAA TAGGTCAGCA TCCAATTGCT 
GTTTTCGGAC CTAAAACCGA AATTGAAATT AAACCCCAAA AAATGGACAT CTCTAATATG 
ATGAATACAT CGGTTCCACC AATGCCAATG GAAGTCGACG AAGTTCCACT GGAGGTATCA 
AACACTTTGG CCAATATGAG CGATCACGCT TATGTGACTG ATATACAGAT ACTACCGTCG 
TTGCAACTCA ATTCTTTGAA TCTTGTTAGC ACCAATATTA CAGACAATGC AGTAAATATC 
GCCCCATCGA CATCAACAAA TGATTTGCAA ACGCCAAGTG ACCGCAGAAC ATCAATGCAA 
AATCAACTAT ATCGAGTGTC CATGAACGAT TTGGCATATA TTTCACAACA CGCTAATGTG 
GTTCGTCTGA TGGTAGATCG TCAAGAGGAA CTAGGGGCAC AGGTCAGCTC CCTGGGGCCT 
TTTAAGGAAA ATAGCCAAGG CGCGCTAAAT TTTAGTACAA TGCCCGCTGT CAAAGTCTAC 
GATCCACGTA TAATTCAGAA CATTCAGAAG GATAAGATGA GAGTAGTTCA TGGGCTCTTC 
GGTGCTCTTA TCAGACAGTC CCAGGTTGAT ATGCACAATG CACCATTCAT CCGAAATCGC 
AAGGATGCGT TGATGGCATA TCGCTTTTTA CTCGAGCTTA AGACTGCAAA TATTATAAGT 
CTTAGCTCGG ATGGTAGATT TTTCGGGCTG CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

solo ( NM_001316394.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303323.1 Drosophila melanogaster sisters on the loose (solo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15891
Clone ID Related Accession (Same CDS sequence)	NM_001032103.2 , NM_001316394.1
Accession Version	NM_001316394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sisters on the loose, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGCTAAGGAA 
CTGCCGCCGA TCCCATCTAG CTGCGATAAG GATGAGGTGG GCGCATTCAT CGATCAGCTG 
GACTTGAGCA AGTACACGAC CAGCCTAGCA CTGATTCGAA AATTGCGTGA CTACTTGCTG 
ACTGCATTTA AGGCTTTGGT GAACCAAGCA TGGGCTTCTC TCGAGAAATT AGCTCAAGAA 
AAGCGTGCAC AAAAGGCACA TCAGATACGC GAAACAACAG ATATGATGAA TCGCCTTATG 
GAGGACATGG GCCGATTGAT TCGTGAAGGT GAACATGAGG AAGCAAATGG GATTGGGATG 
GGATTTCAAA GCATTGATGC GGATTGCAGA GCTGATTTGT CGCAATGGAG GAATTTACAG 
CAAGTTGTGA TGCCGCAATC TTCGGAGATC AGAGATGGAC AAACAAACAG GACAAGCGGT 
ACTGAGGGCG GTCGATCAGG AGGTTTAACC GATGGCGATG ACTTAACCGA AATACAAAGC 
GCCGCATACG CCGTGTATCA TCAGGTTTTG GATCGAGGTT ACCAAACGGG TCGACGGCTG 
TATGATGATA ATCAAGGAGC ATTGAGAGAA GATTCTATTC CAACAGAAGA CCGAGAACAC 
CCAGAAAATA ATCAAGTGGA TGGATCAACA TATGGAGTGA CTAGATTTCT GCACTGGCTA 
AGTGAGAACT TTGTCACTCG GCATGAAATA AGGGCCGTAC GTTTTGGCAG TCCGCATATG 
GTTTTTCAAT CTTATGACTG GCCAGAGTCT CCAAAGCAGC ACATTTCGAC GGTCCATCAA 
AAAGGCATAT TTTATTCTCA GCCCTTAATT AGGACACGCA TAATAGCCAC TGTTGCGAGC 
GAGCCAAACA ATTCGAGTAG TGGTCAGCTC AGCAGTATGG TAAACTTGGC ACGACATCAG 
CGGTCGCAAA ATCAGGAAAC GCCAGATGGA ATTTACGTTA GTCGCTACAA TGCAACTGGA 
GATGAGGAGA CTCCCTGCTC CGCAACCGGA GTCCCCGGCA GAGTTGTGGC TCTGCGTGCT 
TCGAGCACCC CAAATGAACA TAGGAGCGAT CCAGGCTTGT CTTTTATACC CAATTTGACT 
TACGAAGATG GGACCATACC ATCATTGGCA GCAGGCGGCG CTGCATCGAC ACCGTTGGCT 
CCATCACTGC CAGCAGAGCA TTCATTGGGG TTAGAAATGG ATAGCAACAC TTTGGTGGTA 
CCAGCTGATG CGGATGAAAC CCGCTTAGTC GGACATCCAG GATCAACAGT GGTTCGTTCT 
CGGCTTGGAG GCCCTCCAGC ATCAACACCT TTACTATCAT TCCGACCTAG ACTGCCGGTC 
ATTGACGAAG AGCATCATCT ACGCTTTACC AGGGTGTCAC GCGAGCTGCA AATGAGTGTT 
GCTTTCCCGC CGGAAGAGAC AACACTAAGC GAGCAACGTG AGTGGGGCTC GATTTTCCGA 
CCACGTGATG AAATGGTTAA CTATGCCGAA GGTCCACCTC CTCGCCAAAC ACAAAGGGCG 
CGTCGTCGCT CGCGTACACG CAGACGACGT AATCGTATCG CTTCAGCTGC CAGCCCGCAA 
CTAACACCGG AACGAATTGT CCGTCGCGAG GAAGCCTTCC AAAGCAGCCG ACATATTGCA 
GCCACTTTTA TGTCCAGCTT GCTACAAAAT GCAGCTGGAG AAGTGACGAC GTCTTCAGGT 
CTGGCAGTAA CCCGTTCAGT GAAAGAAATA ACGACATCAG TCCATATCAC CCGAAATGTA 
CTACAAGAGG AGGTACCACC GACCACAGAA GCAACGACGA CCTCCCTAGA AGTTGCACAG 
GCAACAGGGT ATTCGGATTC TGGGGCTTGT ATGCAATCTG CAGCGTTCTC TCAAGATCTT 
CCTGTAGTAG CTCCCTTGGA GATATCTGGA AATGCATCAA GTGACCAAGC ATCATTTGAT 
CAAGCTTTGG CCTTACTGCC TCCCATAAAT TCCACTCCAA TAGGTCAGCA TCCAATTGCT 
GTTTTCGGAC CTAAAACCGA AATTGAAATT AAACCCCAAA AAATGGACAT CTCTAATATG 
ATGAATACAT CGGTTCCACC AATGCCAATG GAAGTCGACG AAGTTCCACT GGAGGTATCA 
AACACTTTGG CCAATATGAG CGATCACGCT TATGTGACTG ATATACAGAT ACTACCGTCG 
TTGCAACTCA ATTCTTTGAA TCTTGTTAGC ACCAATATTA CAGACAATGC AGTAAATATC 
GCCCCATCGA CATCAACAAA TGATTTGCAA ACGCCAAGTG ACCGCAGAAC ATCAATGCAA 
AATCAACTAT ATCGAGTGTC CATGAACGAT TTGGCATATA TTTCACAACA CGCTAATGTG 
GTTCGTCTGA TGGTAGATCG TCAAGAGGAA CTAGGGGCAC AGGTCAGCTC CCTGGGGCCT 
TTTAAGGAAA ATAGCCAAGG CGCGCTAAAT TTTAGTACAA TGCCCGCTGT CAAAGTCTAC 
GATCCACGTA TAATTCAGAA CATTCAGAAG GATAAGATGA GAGTAGTTCA TGGGCTCTTC 
GGTGCTCTTA TCAGACAGTC CCAGGTTGAT ATGCACAATG CACCATTCAT CCGAAATCGC 
AAGGATGCGT TGATGGCATA TCGCTTTTTA CTCGAGCTTA AGACTGCAAA TATTATAAGT 
CTTAGCTCGG ATGGTAGATT TTTCGGGCTG CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303323.1
CDS	106..3201
Translation	MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDAKELPPIPSSCDKDEVGAFIDQLDLSKYTTSLALIRKLRDYLLTAFKALVNQAWASLEKLAQEKRAQKAHQIRETTDMMNRLMEDMGRLIREGEHEEANGIGMGFQSIDADCRADLSQWRNLQQVVMPQSSEIRDGQTNRTSGTEGGRSGGLTDGDDLTEIQSAAYAVYHQVLDRGYQTGRRLYDDNQGALREDSIPTEDREHPENNQVDGSTYGVTRFLHWLSENFVTRHEIRAVRFGSPHMVFQSYDWPESPKQHISTVHQKGIFYSQPLIRTRIIATVASEPNNSSSGQLSSMVNLARHQRSQNQETPDGIYVSRYNATGDEETPCSATGVPGRVVALRASSTPNEHRSDPGLSFIPNLTYEDGTIPSLAAGGAASTPLAPSLPAEHSLGLEMDSNTLVVPADADETRLVGHPGSTVVRSRLGGPPASTPLLSFRPRLPVIDEEHHLRFTRVSRELQMSVAFPPEETTLSEQREWGSIFRPRDEMVNYAEGPPPRQTQRARRRSRTRRRRNRIASAASPQLTPERIVRREEAFQSSRHIAATFMSSLLQNAAGEVTTSSGLAVTRSVKEITTSVHITRNVLQEEVPPTTEATTTSLEVAQATGYSDSGACMQSAAFSQDLPVVAPLEISGNASSDQASFDQALALLPPINSTPIGQHPIAVFGPKTEIEIKPQKMDISNMMNTSVPPMPMEVDEVPLEVSNTLANMSDHAYVTDIQILPSLQLNSLNLVSTNITDNAVNIAPSTSTNDLQTPSDRRTSMQNQLYRVSMNDLAYISQHANVVRLMVDRQEELGAQVSSLGPFKENSQGALNFSTMPAVKVYDPRIIQNIQKDKMRVVHGLFGALIRQSQVDMHNAPFIRNRKDALMAYRFLLELKTANIISLSSDGRFFGLL

Target ORF information:

RefSeq Version	NM_001316394.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sisters on the loose (solo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316394.1

ORF Insert Sequence:

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGCTAAGGAA 
CTGCCGCCGA TCCCATCTAG CTGCGATAAG GATGAGGTGG GCGCATTCAT CGATCAGCTG 
GACTTGAGCA AGTACACGAC CAGCCTAGCA CTGATTCGAA AATTGCGTGA CTACTTGCTG 
ACTGCATTTA AGGCTTTGGT GAACCAAGCA TGGGCTTCTC TCGAGAAATT AGCTCAAGAA 
AAGCGTGCAC AAAAGGCACA TCAGATACGC GAAACAACAG ATATGATGAA TCGCCTTATG 
GAGGACATGG GCCGATTGAT TCGTGAAGGT GAACATGAGG AAGCAAATGG GATTGGGATG 
GGATTTCAAA GCATTGATGC GGATTGCAGA GCTGATTTGT CGCAATGGAG GAATTTACAG 
CAAGTTGTGA TGCCGCAATC TTCGGAGATC AGAGATGGAC AAACAAACAG GACAAGCGGT 
ACTGAGGGCG GTCGATCAGG AGGTTTAACC GATGGCGATG ACTTAACCGA AATACAAAGC 
GCCGCATACG CCGTGTATCA TCAGGTTTTG GATCGAGGTT ACCAAACGGG TCGACGGCTG 
TATGATGATA ATCAAGGAGC ATTGAGAGAA GATTCTATTC CAACAGAAGA CCGAGAACAC 
CCAGAAAATA ATCAAGTGGA TGGATCAACA TATGGAGTGA CTAGATTTCT GCACTGGCTA 
AGTGAGAACT TTGTCACTCG GCATGAAATA AGGGCCGTAC GTTTTGGCAG TCCGCATATG 
GTTTTTCAAT CTTATGACTG GCCAGAGTCT CCAAAGCAGC ACATTTCGAC GGTCCATCAA 
AAAGGCATAT TTTATTCTCA GCCCTTAATT AGGACACGCA TAATAGCCAC TGTTGCGAGC 
GAGCCAAACA ATTCGAGTAG TGGTCAGCTC AGCAGTATGG TAAACTTGGC ACGACATCAG 
CGGTCGCAAA ATCAGGAAAC GCCAGATGGA ATTTACGTTA GTCGCTACAA TGCAACTGGA 
GATGAGGAGA CTCCCTGCTC CGCAACCGGA GTCCCCGGCA GAGTTGTGGC TCTGCGTGCT 
TCGAGCACCC CAAATGAACA TAGGAGCGAT CCAGGCTTGT CTTTTATACC CAATTTGACT 
TACGAAGATG GGACCATACC ATCATTGGCA GCAGGCGGCG CTGCATCGAC ACCGTTGGCT 
CCATCACTGC CAGCAGAGCA TTCATTGGGG TTAGAAATGG ATAGCAACAC TTTGGTGGTA 
CCAGCTGATG CGGATGAAAC CCGCTTAGTC GGACATCCAG GATCAACAGT GGTTCGTTCT 
CGGCTTGGAG GCCCTCCAGC ATCAACACCT TTACTATCAT TCCGACCTAG ACTGCCGGTC 
ATTGACGAAG AGCATCATCT ACGCTTTACC AGGGTGTCAC GCGAGCTGCA AATGAGTGTT 
GCTTTCCCGC CGGAAGAGAC AACACTAAGC GAGCAACGTG AGTGGGGCTC GATTTTCCGA 
CCACGTGATG AAATGGTTAA CTATGCCGAA GGTCCACCTC CTCGCCAAAC ACAAAGGGCG 
CGTCGTCGCT CGCGTACACG CAGACGACGT AATCGTATCG CTTCAGCTGC CAGCCCGCAA 
CTAACACCGG AACGAATTGT CCGTCGCGAG GAAGCCTTCC AAAGCAGCCG ACATATTGCA 
GCCACTTTTA TGTCCAGCTT GCTACAAAAT GCAGCTGGAG AAGTGACGAC GTCTTCAGGT 
CTGGCAGTAA CCCGTTCAGT GAAAGAAATA ACGACATCAG TCCATATCAC CCGAAATGTA 
CTACAAGAGG AGGTACCACC GACCACAGAA GCAACGACGA CCTCCCTAGA AGTTGCACAG 
GCAACAGGGT ATTCGGATTC TGGGGCTTGT ATGCAATCTG CAGCGTTCTC TCAAGATCTT 
CCTGTAGTAG CTCCCTTGGA GATATCTGGA AATGCATCAA GTGACCAAGC ATCATTTGAT 
CAAGCTTTGG CCTTACTGCC TCCCATAAAT TCCACTCCAA TAGGTCAGCA TCCAATTGCT 
GTTTTCGGAC CTAAAACCGA AATTGAAATT AAACCCCAAA AAATGGACAT CTCTAATATG 
ATGAATACAT CGGTTCCACC AATGCCAATG GAAGTCGACG AAGTTCCACT GGAGGTATCA 
AACACTTTGG CCAATATGAG CGATCACGCT TATGTGACTG ATATACAGAT ACTACCGTCG 
TTGCAACTCA ATTCTTTGAA TCTTGTTAGC ACCAATATTA CAGACAATGC AGTAAATATC 
GCCCCATCGA CATCAACAAA TGATTTGCAA ACGCCAAGTG ACCGCAGAAC ATCAATGCAA 
AATCAACTAT ATCGAGTGTC CATGAACGAT TTGGCATATA TTTCACAACA CGCTAATGTG 
GTTCGTCTGA TGGTAGATCG TCAAGAGGAA CTAGGGGCAC AGGTCAGCTC CCTGGGGCCT 
TTTAAGGAAA ATAGCCAAGG CGCGCTAAAT TTTAGTACAA TGCCCGCTGT CAAAGTCTAC 
GATCCACGTA TAATTCAGAA CATTCAGAAG GATAAGATGA GAGTAGTTCA TGGGCTCTTC 
GGTGCTCTTA TCAGACAGTC CCAGGTTGAT ATGCACAATG CACCATTCAT CCGAAATCGC 
AAGGATGCGT TGATGGCATA TCGCTTTTTA CTCGAGCTTA AGACTGCAAA TATTATAAGT 
CTTAGCTCGG ATGGTAGATT TTTCGGGCTG CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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solo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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