LOC109546465 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109546465
Entrez Gene ID	109546465
Full Name	irregular chiasm C-roughest protein-like
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019917438.1	XP_019772997.1	irregular chiasm C-roughest protein-like
XM_019917439.1	XP_019772998.1	irregular chiasm C-roughest protein-like

Pathways from BioSystems
Gene Ontology

The following LOC109546465 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109546465 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe35444	XM_019917438.1 Latest version!	Dendroctonus ponderosae irregular chiasm C-roughest protein-like (LOC109546465), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODe35444	XM_019917439.1 Latest version!	Dendroctonus ponderosae irregular chiasm C-roughest protein-like (LOC109546465), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109546465 ( XM_019917438.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019772997.1 Dendroctonus ponderosae irregular chiasm C-roughest protein-like (LOC109546465), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe35444
Clone ID Related Accession (Same CDS sequence)	XM_019917438.1 , XM_019917439.1
Accession Version	XM_019917438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	irregular chiasm C-roughest protein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851153.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG CCTACAAATT GTTCATCTTG CTCCTGCGGA TAGGAGTAAT TTGCTACGTT 
AAAGTGGAGG CTGTGGAGCA AAAGTTTGCG ATGGAACCGC AGGATCAAAC AGCTGTAGTG 
GGATCGCGGG TAACTTTGCC CTGTCGGGTA ATTGGAAAGA TCGGGACATT GCAATGGACG 
AAGGACGACT TTGGGCTGGG AACGCACCGG AACCTGAGTG GATTCGACCG CTACTCGATG 
GTCGGTAGCG ATGAAGAAGG CGATTATTCT TTGGATATTT ATCCAGTAAT GCTTGATGAT 
GACGCCCGGT ATCAATGCCA AGTTTCTCCT GGACCTCAAG GACAGCCAGG TATCAGAAGC 
AATTTTGCTA CGCTGTCTGT CCTCGTACCG CCAGATCCAC CAAAAATTGC CCAAGGAGAC 
CATTTGGTGA CCACAGAGGA CAGAGAAATT GAGTTGGAGT GTATCAGTTC TGGAGGAAAA 
CCGGCGGCGG AAATCACTTG GATAGATGGA CATGGAAATG TCCTAACCGA AGGCAACGAA 
TACATAATCG AAAAGTCGGC GGATAGTCGT AGATTCACTG CCAAATCGAT TTTGAAACTC 
ACACCGAGGA AACTGCACCA CAACACCACT TTTACATGCC AAGCTCAAAA TACCGCTGAA 
CGAACCCATC GAAGTGCAAA ACTCAAGCTA GAGGTGAAAT ACGCTCCGAA GGTAATGGTT 
ACATTTCTCT CTGGAATGAA TGCCAATGGA CAGATTCCTG AAGGATCCGA AATTCGATTA 
GGATGCAAGG CAGACGCAAA CCCGCCTGAT GTTTCATACA AATGGTTTAT GAATGACGAA 
CCTATTATTG GGGATTTTAC AAAAGAGCTC GTTTTACGCA ATGTGTCCAG AAAACAGCAC 
GACGCCATCG TCAAGTGCGA AGTGCACAAT GCAGTTGGAA AAAGTGAAGA AAGCCAAACT 
TTGGACGTCA GCTATGGTCC GCAGTTCCGT ACAAAACCCC GCTCTATTCA AACTGAACTA 
GGTTCGAATG TTGTGCTGAC TTGCGACGTG GACGGCAATC CATTCCCCAA CATAATCTGG 
TACCATGAGG ATAAAAAGGT GGGCACCAGC CCGAATCTGA CTCTGAAAGT CGATCAATCG 
AACATCGGCA AATATTATTG CAAAGCGCAC GTGACCGGAT TTCAAGATAT CGGGGCTGAG 
GCCGCCATTT ACCTGAAAGG TCCTCCATCA ATAGTCTCAC ATCGAACACA ATTTGGAATC 
GAAGGAGACA ACGTGAGAGT TGAATGTGTG GCGTTTTCCA TTCCAGCGCC AGAACGGGTC 
TTTTGGTCCT TTAATGGAAG GGCTGTGGAT CCTCAAAGTC GCGATTATCA CATCGAAGAG 
GTGCCGTTAC CTGAAGGCAT CAAGTCCACT TTGACGATTA GAGAATCGGA GGAAAAACAC 
TTTGGAATGT ACAATTGCAG TGTCAATAAT CCATTTGGAA GCGACACTGT CGAGATCAGT 
CTGATGCCAC AAAAAACATT TCCCATGCTG ATAGTGGTGG TTGGCGTAAT AGGAGGAATT 
ATTCTACTAA TTATCATCAT AATGATCGTG TTTCTCTGCC AAAGGCAGGG CAACAAAAAA 
CCGCCTTCAG ACAATGCGCA CACAATGGAA AAACAATGTA AAGAATCGGA CCGAAGTTCG 
AACATCTCCG ATTTGAAGAT CGACCTGCGA ACTGGATCGT CCAACTCGAA CGTCCACTGC 
GACATGGATT ATATTCCAGG TGCGGAATCC GAAACGGGCA GCGAATCGGT CATCACCAGG 
ATCGGCGTTC CATTGGCCGG TCCTGTGAAC GTCAACAGTC AGGACATGTA CCGATATTCT 
TCGGATTACA CTGAACCGAA TTTCCCGCCA AAGGACGGTC AAAACAACAA CGGTTACGTT 
CCATACGTGG ACTATTCCAG AGACTACAAT CCGCCAATGA TGCAGCCTCC TATTTCGACG 
GCAATGGGCG GTGAAAGTCT TCAGTCGACT CGGCTGCAGT TGAATTCGCT GCAAAACCAT 
CAGCTCAGCA TGGGCGGAAA CGGCAACAGT TTCCCGATGA ACGATCTGTC GGACTTGCAT 
ATGTCGCAGT CGAACGGTTC CATTCCAGGG ATTGATCCTC GGTTCAGTGC CACCTACGGG 
AATCCATATC TTCGGAACCC CAGCGTTGGA TTGCCCACTC CCAACACGGC CAATCCAGTC 
GCAACTCCTG CTCCTCCTCC ATACAGCAGG GAGAACTCGA GATTGGCCAT ATCGAGTAGT 
GGGATGATCA CTGCTAGTGC AAATCCTGTG AACACTGTAG TGTCGAACGG TGCCAATGGC 
AGTGTTCCTC AAGTGACGAG ACTGCCCAGT TCACCTACCA GTCAGTATAT TGTGCCCAGC 
TCGACAATAA AACGAGGGAC TTTGGCTACC CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019772997.1
CDS	528..2966
Translation	MTSAYKLFILLLRIGVICYVKVEAVEQKFAMEPQDQTAVVGSRVTLPCRVIGKIGTLQWTKDDFGLGTHRNLSGFDRYSMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPQGQPGIRSNFATLSVLVPPDPPKIAQGDHLVTTEDREIELECISSGGKPAAEITWIDGHGNVLTEGNEYIIEKSADSRRFTAKSILKLTPRKLHHNTTFTCQAQNTAERTHRSAKLKLEVKYAPKVMVTFLSGMNANGQIPEGSEIRLGCKADANPPDVSYKWFMNDEPIIGDFTKELVLRNVSRKQHDAIVKCEVHNAVGKSEESQTLDVSYGPQFRTKPRSIQTELGSNVVLTCDVDGNPFPNIIWYHEDKKVGTSPNLTLKVDQSNIGKYYCKAHVTGFQDIGAEAAIYLKGPPSIVSHRTQFGIEGDNVRVECVAFSIPAPERVFWSFNGRAVDPQSRDYHIEEVPLPEGIKSTLTIRESEEKHFGMYNCSVNNPFGSDTVEISLMPQKTFPMLIVVVGVIGGIILLIIIIMIVFLCQRQGNKKPPSDNAHTMEKQCKESDRSSNISDLKIDLRTGSSNSNVHCDMDYIPGAESETGSESVITRIGVPLAGPVNVNSQDMYRYSSDYTEPNFPPKDGQNNNGYVPYVDYSRDYNPPMMQPPISTAMGGESLQSTRLQLNSLQNHQLSMGGNGNSFPMNDLSDLHMSQSNGSIPGIDPRFSATYGNPYLRNPSVGLPTPNTANPVATPAPPPYSRENSRLAISSSGMITASANPVNTVVSNGANGSVPQVTRLPSSPTSQYIVPSSTIKRGTLATHV

Target ORF information:

RefSeq Version	XM_019917438.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae irregular chiasm C-roughest protein-like (LOC109546465), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917438.1

ORF Insert Sequence:

ATGACTTCGG CCTACAAATT GTTCATCTTG CTCCTGCGGA TAGGAGTAAT TTGCTACGTT 
AAAGTGGAGG CTGTGGAGCA AAAGTTTGCG ATGGAACCGC AGGATCAAAC AGCTGTAGTG 
GGATCGCGGG TAACTTTGCC CTGTCGGGTA ATTGGAAAGA TCGGGACATT GCAATGGACG 
AAGGACGACT TTGGGCTGGG AACGCACCGG AACCTGAGTG GATTCGACCG CTACTCGATG 
GTCGGTAGCG ATGAAGAAGG CGATTATTCT TTGGATATTT ATCCAGTAAT GCTTGATGAT 
GACGCCCGGT ATCAATGCCA AGTTTCTCCT GGACCTCAAG GACAGCCAGG TATCAGAAGC 
AATTTTGCTA CGCTGTCTGT CCTCGTACCG CCAGATCCAC CAAAAATTGC CCAAGGAGAC 
CATTTGGTGA CCACAGAGGA CAGAGAAATT GAGTTGGAGT GTATCAGTTC TGGAGGAAAA 
CCGGCGGCGG AAATCACTTG GATAGATGGA CATGGAAATG TCCTAACCGA AGGCAACGAA 
TACATAATCG AAAAGTCGGC GGATAGTCGT AGATTCACTG CCAAATCGAT TTTGAAACTC 
ACACCGAGGA AACTGCACCA CAACACCACT TTTACATGCC AAGCTCAAAA TACCGCTGAA 
CGAACCCATC GAAGTGCAAA ACTCAAGCTA GAGGTGAAAT ACGCTCCGAA GGTAATGGTT 
ACATTTCTCT CTGGAATGAA TGCCAATGGA CAGATTCCTG AAGGATCCGA AATTCGATTA 
GGATGCAAGG CAGACGCAAA CCCGCCTGAT GTTTCATACA AATGGTTTAT GAATGACGAA 
CCTATTATTG GGGATTTTAC AAAAGAGCTC GTTTTACGCA ATGTGTCCAG AAAACAGCAC 
GACGCCATCG TCAAGTGCGA AGTGCACAAT GCAGTTGGAA AAAGTGAAGA AAGCCAAACT 
TTGGACGTCA GCTATGGTCC GCAGTTCCGT ACAAAACCCC GCTCTATTCA AACTGAACTA 
GGTTCGAATG TTGTGCTGAC TTGCGACGTG GACGGCAATC CATTCCCCAA CATAATCTGG 
TACCATGAGG ATAAAAAGGT GGGCACCAGC CCGAATCTGA CTCTGAAAGT CGATCAATCG 
AACATCGGCA AATATTATTG CAAAGCGCAC GTGACCGGAT TTCAAGATAT CGGGGCTGAG 
GCCGCCATTT ACCTGAAAGG TCCTCCATCA ATAGTCTCAC ATCGAACACA ATTTGGAATC 
GAAGGAGACA ACGTGAGAGT TGAATGTGTG GCGTTTTCCA TTCCAGCGCC AGAACGGGTC 
TTTTGGTCCT TTAATGGAAG GGCTGTGGAT CCTCAAAGTC GCGATTATCA CATCGAAGAG 
GTGCCGTTAC CTGAAGGCAT CAAGTCCACT TTGACGATTA GAGAATCGGA GGAAAAACAC 
TTTGGAATGT ACAATTGCAG TGTCAATAAT CCATTTGGAA GCGACACTGT CGAGATCAGT 
CTGATGCCAC AAAAAACATT TCCCATGCTG ATAGTGGTGG TTGGCGTAAT AGGAGGAATT 
ATTCTACTAA TTATCATCAT AATGATCGTG TTTCTCTGCC AAAGGCAGGG CAACAAAAAA 
CCGCCTTCAG ACAATGCGCA CACAATGGAA AAACAATGTA AAGAATCGGA CCGAAGTTCG 
AACATCTCCG ATTTGAAGAT CGACCTGCGA ACTGGATCGT CCAACTCGAA CGTCCACTGC 
GACATGGATT ATATTCCAGG TGCGGAATCC GAAACGGGCA GCGAATCGGT CATCACCAGG 
ATCGGCGTTC CATTGGCCGG TCCTGTGAAC GTCAACAGTC AGGACATGTA CCGATATTCT 
TCGGATTACA CTGAACCGAA TTTCCCGCCA AAGGACGGTC AAAACAACAA CGGTTACGTT 
CCATACGTGG ACTATTCCAG AGACTACAAT CCGCCAATGA TGCAGCCTCC TATTTCGACG 
GCAATGGGCG GTGAAAGTCT TCAGTCGACT CGGCTGCAGT TGAATTCGCT GCAAAACCAT 
CAGCTCAGCA TGGGCGGAAA CGGCAACAGT TTCCCGATGA ACGATCTGTC GGACTTGCAT 
ATGTCGCAGT CGAACGGTTC CATTCCAGGG ATTGATCCTC GGTTCAGTGC CACCTACGGG 
AATCCATATC TTCGGAACCC CAGCGTTGGA TTGCCCACTC CCAACACGGC CAATCCAGTC 
GCAACTCCTG CTCCTCCTCC ATACAGCAGG GAGAACTCGA GATTGGCCAT ATCGAGTAGT 
GGGATGATCA CTGCTAGTGC AAATCCTGTG AACACTGTAG TGTCGAACGG TGCCAATGGC 
AGTGTTCCTC AAGTGACGAG ACTGCCCAGT TCACCTACCA GTCAGTATAT TGTGCCCAGC 
TCGACAATAA AACGAGGGAC TTTGGCTACC CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109546465 ( XM_019917439.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019772998.1 Dendroctonus ponderosae irregular chiasm C-roughest protein-like (LOC109546465), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe35444
Clone ID Related Accession (Same CDS sequence)	XM_019917438.1 , XM_019917439.1
Accession Version	XM_019917439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	irregular chiasm C-roughest protein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851153.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG CCTACAAATT GTTCATCTTG CTCCTGCGGA TAGGAGTAAT TTGCTACGTT 
AAAGTGGAGG CTGTGGAGCA AAAGTTTGCG ATGGAACCGC AGGATCAAAC AGCTGTAGTG 
GGATCGCGGG TAACTTTGCC CTGTCGGGTA ATTGGAAAGA TCGGGACATT GCAATGGACG 
AAGGACGACT TTGGGCTGGG AACGCACCGG AACCTGAGTG GATTCGACCG CTACTCGATG 
GTCGGTAGCG ATGAAGAAGG CGATTATTCT TTGGATATTT ATCCAGTAAT GCTTGATGAT 
GACGCCCGGT ATCAATGCCA AGTTTCTCCT GGACCTCAAG GACAGCCAGG TATCAGAAGC 
AATTTTGCTA CGCTGTCTGT CCTCGTACCG CCAGATCCAC CAAAAATTGC CCAAGGAGAC 
CATTTGGTGA CCACAGAGGA CAGAGAAATT GAGTTGGAGT GTATCAGTTC TGGAGGAAAA 
CCGGCGGCGG AAATCACTTG GATAGATGGA CATGGAAATG TCCTAACCGA AGGCAACGAA 
TACATAATCG AAAAGTCGGC GGATAGTCGT AGATTCACTG CCAAATCGAT TTTGAAACTC 
ACACCGAGGA AACTGCACCA CAACACCACT TTTACATGCC AAGCTCAAAA TACCGCTGAA 
CGAACCCATC GAAGTGCAAA ACTCAAGCTA GAGGTGAAAT ACGCTCCGAA GGTAATGGTT 
ACATTTCTCT CTGGAATGAA TGCCAATGGA CAGATTCCTG AAGGATCCGA AATTCGATTA 
GGATGCAAGG CAGACGCAAA CCCGCCTGAT GTTTCATACA AATGGTTTAT GAATGACGAA 
CCTATTATTG GGGATTTTAC AAAAGAGCTC GTTTTACGCA ATGTGTCCAG AAAACAGCAC 
GACGCCATCG TCAAGTGCGA AGTGCACAAT GCAGTTGGAA AAAGTGAAGA AAGCCAAACT 
TTGGACGTCA GCTATGGTCC GCAGTTCCGT ACAAAACCCC GCTCTATTCA AACTGAACTA 
GGTTCGAATG TTGTGCTGAC TTGCGACGTG GACGGCAATC CATTCCCCAA CATAATCTGG 
TACCATGAGG ATAAAAAGGT GGGCACCAGC CCGAATCTGA CTCTGAAAGT CGATCAATCG 
AACATCGGCA AATATTATTG CAAAGCGCAC GTGACCGGAT TTCAAGATAT CGGGGCTGAG 
GCCGCCATTT ACCTGAAAGG TCCTCCATCA ATAGTCTCAC ATCGAACACA ATTTGGAATC 
GAAGGAGACA ACGTGAGAGT TGAATGTGTG GCGTTTTCCA TTCCAGCGCC AGAACGGGTC 
TTTTGGTCCT TTAATGGAAG GGCTGTGGAT CCTCAAAGTC GCGATTATCA CATCGAAGAG 
GTGCCGTTAC CTGAAGGCAT CAAGTCCACT TTGACGATTA GAGAATCGGA GGAAAAACAC 
TTTGGAATGT ACAATTGCAG TGTCAATAAT CCATTTGGAA GCGACACTGT CGAGATCAGT 
CTGATGCCAC AAAAAACATT TCCCATGCTG ATAGTGGTGG TTGGCGTAAT AGGAGGAATT 
ATTCTACTAA TTATCATCAT AATGATCGTG TTTCTCTGCC AAAGGCAGGG CAACAAAAAA 
CCGCCTTCAG ACAATGCGCA CACAATGGAA AAACAATGTA AAGAATCGGA CCGAAGTTCG 
AACATCTCCG ATTTGAAGAT CGACCTGCGA ACTGGATCGT CCAACTCGAA CGTCCACTGC 
GACATGGATT ATATTCCAGG TGCGGAATCC GAAACGGGCA GCGAATCGGT CATCACCAGG 
ATCGGCGTTC CATTGGCCGG TCCTGTGAAC GTCAACAGTC AGGACATGTA CCGATATTCT 
TCGGATTACA CTGAACCGAA TTTCCCGCCA AAGGACGGTC AAAACAACAA CGGTTACGTT 
CCATACGTGG ACTATTCCAG AGACTACAAT CCGCCAATGA TGCAGCCTCC TATTTCGACG 
GCAATGGGCG GTGAAAGTCT TCAGTCGACT CGGCTGCAGT TGAATTCGCT GCAAAACCAT 
CAGCTCAGCA TGGGCGGAAA CGGCAACAGT TTCCCGATGA ACGATCTGTC GGACTTGCAT 
ATGTCGCAGT CGAACGGTTC CATTCCAGGG ATTGATCCTC GGTTCAGTGC CACCTACGGG 
AATCCATATC TTCGGAACCC CAGCGTTGGA TTGCCCACTC CCAACACGGC CAATCCAGTC 
GCAACTCCTG CTCCTCCTCC ATACAGCAGG GAGAACTCGA GATTGGCCAT ATCGAGTAGT 
GGGATGATCA CTGCTAGTGC AAATCCTGTG AACACTGTAG TGTCGAACGG TGCCAATGGC 
AGTGTTCCTC AAGTGACGAG ACTGCCCAGT TCACCTACCA GTCAGTATAT TGTGCCCAGC 
TCGACAATAA AACGAGGGAC TTTGGCTACC CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019772998.1
CDS	570..3008
Translation	MTSAYKLFILLLRIGVICYVKVEAVEQKFAMEPQDQTAVVGSRVTLPCRVIGKIGTLQWTKDDFGLGTHRNLSGFDRYSMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPQGQPGIRSNFATLSVLVPPDPPKIAQGDHLVTTEDREIELECISSGGKPAAEITWIDGHGNVLTEGNEYIIEKSADSRRFTAKSILKLTPRKLHHNTTFTCQAQNTAERTHRSAKLKLEVKYAPKVMVTFLSGMNANGQIPEGSEIRLGCKADANPPDVSYKWFMNDEPIIGDFTKELVLRNVSRKQHDAIVKCEVHNAVGKSEESQTLDVSYGPQFRTKPRSIQTELGSNVVLTCDVDGNPFPNIIWYHEDKKVGTSPNLTLKVDQSNIGKYYCKAHVTGFQDIGAEAAIYLKGPPSIVSHRTQFGIEGDNVRVECVAFSIPAPERVFWSFNGRAVDPQSRDYHIEEVPLPEGIKSTLTIRESEEKHFGMYNCSVNNPFGSDTVEISLMPQKTFPMLIVVVGVIGGIILLIIIIMIVFLCQRQGNKKPPSDNAHTMEKQCKESDRSSNISDLKIDLRTGSSNSNVHCDMDYIPGAESETGSESVITRIGVPLAGPVNVNSQDMYRYSSDYTEPNFPPKDGQNNNGYVPYVDYSRDYNPPMMQPPISTAMGGESLQSTRLQLNSLQNHQLSMGGNGNSFPMNDLSDLHMSQSNGSIPGIDPRFSATYGNPYLRNPSVGLPTPNTANPVATPAPPPYSRENSRLAISSSGMITASANPVNTVVSNGANGSVPQVTRLPSSPTSQYIVPSSTIKRGTLATHV

Target ORF information:

RefSeq Version	XM_019917439.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae irregular chiasm C-roughest protein-like (LOC109546465), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917439.1

ORF Insert Sequence:

ATGACTTCGG CCTACAAATT GTTCATCTTG CTCCTGCGGA TAGGAGTAAT TTGCTACGTT 
AAAGTGGAGG CTGTGGAGCA AAAGTTTGCG ATGGAACCGC AGGATCAAAC AGCTGTAGTG 
GGATCGCGGG TAACTTTGCC CTGTCGGGTA ATTGGAAAGA TCGGGACATT GCAATGGACG 
AAGGACGACT TTGGGCTGGG AACGCACCGG AACCTGAGTG GATTCGACCG CTACTCGATG 
GTCGGTAGCG ATGAAGAAGG CGATTATTCT TTGGATATTT ATCCAGTAAT GCTTGATGAT 
GACGCCCGGT ATCAATGCCA AGTTTCTCCT GGACCTCAAG GACAGCCAGG TATCAGAAGC 
AATTTTGCTA CGCTGTCTGT CCTCGTACCG CCAGATCCAC CAAAAATTGC CCAAGGAGAC 
CATTTGGTGA CCACAGAGGA CAGAGAAATT GAGTTGGAGT GTATCAGTTC TGGAGGAAAA 
CCGGCGGCGG AAATCACTTG GATAGATGGA CATGGAAATG TCCTAACCGA AGGCAACGAA 
TACATAATCG AAAAGTCGGC GGATAGTCGT AGATTCACTG CCAAATCGAT TTTGAAACTC 
ACACCGAGGA AACTGCACCA CAACACCACT TTTACATGCC AAGCTCAAAA TACCGCTGAA 
CGAACCCATC GAAGTGCAAA ACTCAAGCTA GAGGTGAAAT ACGCTCCGAA GGTAATGGTT 
ACATTTCTCT CTGGAATGAA TGCCAATGGA CAGATTCCTG AAGGATCCGA AATTCGATTA 
GGATGCAAGG CAGACGCAAA CCCGCCTGAT GTTTCATACA AATGGTTTAT GAATGACGAA 
CCTATTATTG GGGATTTTAC AAAAGAGCTC GTTTTACGCA ATGTGTCCAG AAAACAGCAC 
GACGCCATCG TCAAGTGCGA AGTGCACAAT GCAGTTGGAA AAAGTGAAGA AAGCCAAACT 
TTGGACGTCA GCTATGGTCC GCAGTTCCGT ACAAAACCCC GCTCTATTCA AACTGAACTA 
GGTTCGAATG TTGTGCTGAC TTGCGACGTG GACGGCAATC CATTCCCCAA CATAATCTGG 
TACCATGAGG ATAAAAAGGT GGGCACCAGC CCGAATCTGA CTCTGAAAGT CGATCAATCG 
AACATCGGCA AATATTATTG CAAAGCGCAC GTGACCGGAT TTCAAGATAT CGGGGCTGAG 
GCCGCCATTT ACCTGAAAGG TCCTCCATCA ATAGTCTCAC ATCGAACACA ATTTGGAATC 
GAAGGAGACA ACGTGAGAGT TGAATGTGTG GCGTTTTCCA TTCCAGCGCC AGAACGGGTC 
TTTTGGTCCT TTAATGGAAG GGCTGTGGAT CCTCAAAGTC GCGATTATCA CATCGAAGAG 
GTGCCGTTAC CTGAAGGCAT CAAGTCCACT TTGACGATTA GAGAATCGGA GGAAAAACAC 
TTTGGAATGT ACAATTGCAG TGTCAATAAT CCATTTGGAA GCGACACTGT CGAGATCAGT 
CTGATGCCAC AAAAAACATT TCCCATGCTG ATAGTGGTGG TTGGCGTAAT AGGAGGAATT 
ATTCTACTAA TTATCATCAT AATGATCGTG TTTCTCTGCC AAAGGCAGGG CAACAAAAAA 
CCGCCTTCAG ACAATGCGCA CACAATGGAA AAACAATGTA AAGAATCGGA CCGAAGTTCG 
AACATCTCCG ATTTGAAGAT CGACCTGCGA ACTGGATCGT CCAACTCGAA CGTCCACTGC 
GACATGGATT ATATTCCAGG TGCGGAATCC GAAACGGGCA GCGAATCGGT CATCACCAGG 
ATCGGCGTTC CATTGGCCGG TCCTGTGAAC GTCAACAGTC AGGACATGTA CCGATATTCT 
TCGGATTACA CTGAACCGAA TTTCCCGCCA AAGGACGGTC AAAACAACAA CGGTTACGTT 
CCATACGTGG ACTATTCCAG AGACTACAAT CCGCCAATGA TGCAGCCTCC TATTTCGACG 
GCAATGGGCG GTGAAAGTCT TCAGTCGACT CGGCTGCAGT TGAATTCGCT GCAAAACCAT 
CAGCTCAGCA TGGGCGGAAA CGGCAACAGT TTCCCGATGA ACGATCTGTC GGACTTGCAT 
ATGTCGCAGT CGAACGGTTC CATTCCAGGG ATTGATCCTC GGTTCAGTGC CACCTACGGG 
AATCCATATC TTCGGAACCC CAGCGTTGGA TTGCCCACTC CCAACACGGC CAATCCAGTC 
GCAACTCCTG CTCCTCCTCC ATACAGCAGG GAGAACTCGA GATTGGCCAT ATCGAGTAGT 
GGGATGATCA CTGCTAGTGC AAATCCTGTG AACACTGTAG TGTCGAACGG TGCCAATGGC 
AGTGTTCCTC AAGTGACGAG ACTGCCCAGT TCACCTACCA GTCAGTATAT TGTGCCCAGC 
TCGACAATAA AACGAGGGAC TTTGGCTACC CATGTGTAA