LOC109546449 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109546449
Entrez Gene ID	109546449
Full Name	pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019917414.1	XP_019772973.1	pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like

Pathways from BioSystems
Gene Ontology

The following LOC109546449 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109546449 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe35423	XM_019917414.1 Latest version!	Dendroctonus ponderosae pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like (LOC109546449), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109546449 ( XM_019917414.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019772973.1 Dendroctonus ponderosae pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like (LOC109546449), mRNA.

Price & Availability↑

CloneID	ODe35423
Clone ID Related Accession (Same CDS sequence)	XM_019917414.1
Accession Version	XM_019917414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851153.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGATGA CCCAACAAAT CTGCCTGCCA AAACTGACAA TGTGGACAAT AAAAGTCCCT 
GTGAGAATTG CAAAGCGAAT TAGCCAATTT CAGGCTAGAA CTCTTGTGAT CAATGTCGAT 
CAAATGTTCG ATAGCAAAGT GTATAAACTG CATCTTCTGG AGGATGGCCC TTCACACACG 
TCTGAGCTGA CGAAGGGGGA TGCCTTGCGC TTTTACCAGC AGATGGATGA GATTAGGAAA 
GTTGAAAACT CCATCGCTCA GCTGTACAGG GAGAAGAAAA TCAGAGGTTT TTGCCATCTT 
TATGTGGGTC AGGAAGCTGT AGCAGTGGGA ATTAACTCGA TTATGAAACC CGAGGACACT 
GTGATTACTT CATATAGATG CCACGGTTGG GCGGTGTTAA AGTGCGAGTC TGTGGCTTCA 
GTTATAGCAG AACTGATTGG GAATAAATCG GGCGCTTCAA GAGGCAAGGG AGGCTCAATG 
CATATCTACG GCCCTCAATT TTTCGGAGGA AATGGAATAG TTGGCAGCCA CGTTCCTTTG 
GGCGTGGGCA TCGGTTTGAA GCATAGATAT TTGGATAATA AGGCCATATC GGTTACCCTT 
TATGGAGATG GAGCGGCTAA TCAAGGACAA GTGTTCGAGG CTTTCAATCT GGCCAAGCTC 
CTGAATATCC CAGTGCTTTT CGTCGTTGAA AACAACCAAT ACGGACTTGG GACCGCATGC 
AGTAGAAGTT CGGCAAATAA TGAATACTAC AGACAAAGCT CCTTCCTGCC CGGAATACGA 
GCAAGCGGGA TGGACATACT CAGTGTTATT GAAGCTGCCA AATATTGCTT CAAGCATATC 
CAGGACGGCA AAGGCCCCAT AATTTTGGAG ATGATGACCT ATCGGTATTT TGGACACTCC 
ATGTCCGACC CTGGAACCAG CTATAGAGAT CGGAGTGAAA TCCAAGAGGT CAGAGAGAAG 
AAAGATTGCA TTCATCACTT GAAGCAGTTG ATTTTGGATA GTAATTTGGC AAAGGAGGAC 
GAAATTAAAG CGATTGAGAA TGAAAACAAA GAAAAAGTTG CCAAAGCAAC AGAAGAAGCG 
ATTAAGGGAA GTCCTCCTTC TTTGGAAGAA CTCACAGCGG ACCATTATGC CGTTTGGTCA 
GAGAAAGTCC GAATTCCCAG AATGCTGGAA TGGGTACCAT ATAAAACCAT TGCCCATAAT 
AAACCCCCTA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019772973.1
CDS	56..1270
Translation	MSMTQQICLPKLTMWTIKVPVRIAKRISQFQARTLVINVDQMFDSKVYKLHLLEDGPSHTSELTKGDALRFYQQMDEIRKVENSIAQLYREKKIRGFCHLYVGQEAVAVGINSIMKPEDTVITSYRCHGWAVLKCESVASVIAELIGNKSGASRGKGGSMHIYGPQFFGGNGIVGSHVPLGVGIGLKHRYLDNKAISVTLYGDGAANQGQVFEAFNLAKLLNIPVLFVVENNQYGLGTACSRSSANNEYYRQSSFLPGIRASGMDILSVIEAAKYCFKHIQDGKGPIILEMMTYRYFGHSMSDPGTSYRDRSEIQEVREKKDCIHHLKQLILDSNLAKEDEIKAIENENKEKVAKATEEAIKGSPPSLEELTADHYAVWSEKVRIPRMLEWVPYKTIAHNKPPK

Target ORF information:

RefSeq Version	XM_019917414.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like (LOC109546449), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917414.1

ORF Insert Sequence:

ATGTCGATGA CCCAACAAAT CTGCCTGCCA AAACTGACAA TGTGGACAAT AAAAGTCCCT 
GTGAGAATTG CAAAGCGAAT TAGCCAATTT CAGGCTAGAA CTCTTGTGAT CAATGTCGAT 
CAAATGTTCG ATAGCAAAGT GTATAAACTG CATCTTCTGG AGGATGGCCC TTCACACACG 
TCTGAGCTGA CGAAGGGGGA TGCCTTGCGC TTTTACCAGC AGATGGATGA GATTAGGAAA 
GTTGAAAACT CCATCGCTCA GCTGTACAGG GAGAAGAAAA TCAGAGGTTT TTGCCATCTT 
TATGTGGGTC AGGAAGCTGT AGCAGTGGGA ATTAACTCGA TTATGAAACC CGAGGACACT 
GTGATTACTT CATATAGATG CCACGGTTGG GCGGTGTTAA AGTGCGAGTC TGTGGCTTCA 
GTTATAGCAG AACTGATTGG GAATAAATCG GGCGCTTCAA GAGGCAAGGG AGGCTCAATG 
CATATCTACG GCCCTCAATT TTTCGGAGGA AATGGAATAG TTGGCAGCCA CGTTCCTTTG 
GGCGTGGGCA TCGGTTTGAA GCATAGATAT TTGGATAATA AGGCCATATC GGTTACCCTT 
TATGGAGATG GAGCGGCTAA TCAAGGACAA GTGTTCGAGG CTTTCAATCT GGCCAAGCTC 
CTGAATATCC CAGTGCTTTT CGTCGTTGAA AACAACCAAT ACGGACTTGG GACCGCATGC 
AGTAGAAGTT CGGCAAATAA TGAATACTAC AGACAAAGCT CCTTCCTGCC CGGAATACGA 
GCAAGCGGGA TGGACATACT CAGTGTTATT GAAGCTGCCA AATATTGCTT CAAGCATATC 
CAGGACGGCA AAGGCCCCAT AATTTTGGAG ATGATGACCT ATCGGTATTT TGGACACTCC 
ATGTCCGACC CTGGAACCAG CTATAGAGAT CGGAGTGAAA TCCAAGAGGT CAGAGAGAAG 
AAAGATTGCA TTCATCACTT GAAGCAGTTG ATTTTGGATA GTAATTTGGC AAAGGAGGAC 
GAAATTAAAG CGATTGAGAA TGAAAACAAA GAAAAAGTTG CCAAAGCAAC AGAAGAAGCG 
ATTAAGGGAA GTCCTCCTTC TTTGGAAGAA CTCACAGCGG ACCATTATGC CGTTTGGTCA 
GAGAAAGTCC GAATTCCCAG AATGCTGGAA TGGGTACCAT ATAAAACCAT TGCCCATAAT 
AAACCCCCTA AGTAA