LOC109537115 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109537115
Entrez Gene ID	109537115
Full Name	ankyrin repeat domain-containing protein 50
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019903696.1	XP_019759255.1	ankyrin repeat domain-containing protein 50 isoform X2
XM_019903698.1	XP_019759257.1	ankyrin repeat domain-containing protein 50 isoform X4
XM_019903693.1	XP_019759252.1	ankyrin repeat domain-containing protein 50 isoform X1
XM_019903697.1	XP_019759256.1	ankyrin repeat domain-containing protein 50 isoform X3
XM_019903699.1	XP_019759258.1	ankyrin repeat domain-containing protein 50 isoform X5
XM_019903694.1	XP_019759253.1	ankyrin repeat domain-containing protein 50 isoform X1
XM_019903695.1	XP_019759254.1	ankyrin repeat domain-containing protein 50 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC109537115 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109537115 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe40658	XM_019903693.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe40658	XM_019903694.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe40658	XM_019903695.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe40659	XM_019903696.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe40660	XM_019903697.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe40661	XM_019903698.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODe40662	XM_019903699.1 Latest version!	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109537115 ( XM_019903693.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759252.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe40658
Clone ID Related Accession (Same CDS sequence)	XM_019903693.1 , XM_019903694.1 , XM_019903695.1
Accession Version	XM_019903693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCAGTGC ATTTTTAGCA TCGACTGCCG AAACCAAACC TTTATCGGCC 
AGTTTGTATC CGGTAGGCGG AGATTGGACC GATCAGCAAC GAAGATCAAT GGTTTCTTTG 
GGTAATCATA GCTCTAATTC AAAGTCCAGT TCGAATCTGA CGGGCTCAAG CCATTCCAGC 
CGCCATGGCG ATAGGCAGCG AGATACACAA CAGAATACTC AGCCGATGTC TTTCACCCAA 
CAGCTCCAGC AGTGTTCTAG AGGTGGCAAA ACAAGACCGT TGAGTAAACT GTTGTCGCCT 
TTGCAAAGCG AGCCTCAAAG TCCTATTTAC GCCTCACCGC CTCTTAGTCC GGTACATGAC 
AGTTCTAATA ATGTTTTCGG TGCCATGAAT ATTTATCAGC CGGTACTGCG ACATAACAAC 
TTTGCAAAAG CGCCCGACAT GCACTTTGCT CGAGACACCC ATATGCGCAT AATTTTGGGC 
AATTCCGGCA GTTCCGCATT CGGAAAGGAC AAAAAAGGCG ACGAGAAGCA AGACAAGGAT 
TCAAATAATA CCAAACAGAG CAATCAAAAA TCGAAGCGAA ATGGTATTGC AACGAATCCC 
GCATTACGAT TGGTGGCGAA TGTGAAGAAC GGGTTCGATT CGGCAGCAGC CAATTTGCGT 
AAGTCGACAT CTGGAGCGAA TCCAGCCTCG AAAACGAACA GTTTTCATTG GCGGAAAGAG 
ACGCCTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759252.1
CDS	377..5128
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKNTALKQTKDGPLDKKQFKSGLINLLCKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQGCVEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASSAFLASTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903693.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903693.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCAGTGC ATTTTTAGCA TCGACTGCCG AAACCAAACC TTTATCGGCC 
AGTTTGTATC CGGTAGGCGG AGATTGGACC GATCAGCAAC GAAGATCAAT GGTTTCTTTG 
GGTAATCATA GCTCTAATTC AAAGTCCAGT TCGAATCTGA CGGGCTCAAG CCATTCCAGC 
CGCCATGGCG ATAGGCAGCG AGATACACAA CAGAATACTC AGCCGATGTC TTTCACCCAA 
CAGCTCCAGC AGTGTTCTAG AGGTGGCAAA ACAAGACCGT TGAGTAAACT GTTGTCGCCT 
TTGCAAAGCG AGCCTCAAAG TCCTATTTAC GCCTCACCGC CTCTTAGTCC GGTACATGAC 
AGTTCTAATA ATGTTTTCGG TGCCATGAAT ATTTATCAGC CGGTACTGCG ACATAACAAC 
TTTGCAAAAG CGCCCGACAT GCACTTTGCT CGAGACACCC ATATGCGCAT AATTTTGGGC 
AATTCCGGCA GTTCCGCATT CGGAAAGGAC AAAAAAGGCG ACGAGAAGCA AGACAAGGAT 
TCAAATAATA CCAAACAGAG CAATCAAAAA TCGAAGCGAA ATGGTATTGC AACGAATCCC 
GCATTACGAT TGGTGGCGAA TGTGAAGAAC GGGTTCGATT CGGCAGCAGC CAATTTGCGT 
AAGTCGACAT CTGGAGCGAA TCCAGCCTCG AAAACGAACA GTTTTCATTG GCGGAAAGAG 
ACGCCTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109537115 ( XM_019903694.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759253.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe40658
Clone ID Related Accession (Same CDS sequence)	XM_019903693.1 , XM_019903694.1 , XM_019903695.1
Accession Version	XM_019903694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCAGTGC ATTTTTAGCA TCGACTGCCG AAACCAAACC TTTATCGGCC 
AGTTTGTATC CGGTAGGCGG AGATTGGACC GATCAGCAAC GAAGATCAAT GGTTTCTTTG 
GGTAATCATA GCTCTAATTC AAAGTCCAGT TCGAATCTGA CGGGCTCAAG CCATTCCAGC 
CGCCATGGCG ATAGGCAGCG AGATACACAA CAGAATACTC AGCCGATGTC TTTCACCCAA 
CAGCTCCAGC AGTGTTCTAG AGGTGGCAAA ACAAGACCGT TGAGTAAACT GTTGTCGCCT 
TTGCAAAGCG AGCCTCAAAG TCCTATTTAC GCCTCACCGC CTCTTAGTCC GGTACATGAC 
AGTTCTAATA ATGTTTTCGG TGCCATGAAT ATTTATCAGC CGGTACTGCG ACATAACAAC 
TTTGCAAAAG CGCCCGACAT GCACTTTGCT CGAGACACCC ATATGCGCAT AATTTTGGGC 
AATTCCGGCA GTTCCGCATT CGGAAAGGAC AAAAAAGGCG ACGAGAAGCA AGACAAGGAT 
TCAAATAATA CCAAACAGAG CAATCAAAAA TCGAAGCGAA ATGGTATTGC AACGAATCCC 
GCATTACGAT TGGTGGCGAA TGTGAAGAAC GGGTTCGATT CGGCAGCAGC CAATTTGCGT 
AAGTCGACAT CTGGAGCGAA TCCAGCCTCG AAAACGAACA GTTTTCATTG GCGGAAAGAG 
ACGCCTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759253.1
CDS	377..5128
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKNTALKQTKDGPLDKKQFKSGLINLLCKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQGCVEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASSAFLASTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903694.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903694.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCAGTGC ATTTTTAGCA TCGACTGCCG AAACCAAACC TTTATCGGCC 
AGTTTGTATC CGGTAGGCGG AGATTGGACC GATCAGCAAC GAAGATCAAT GGTTTCTTTG 
GGTAATCATA GCTCTAATTC AAAGTCCAGT TCGAATCTGA CGGGCTCAAG CCATTCCAGC 
CGCCATGGCG ATAGGCAGCG AGATACACAA CAGAATACTC AGCCGATGTC TTTCACCCAA 
CAGCTCCAGC AGTGTTCTAG AGGTGGCAAA ACAAGACCGT TGAGTAAACT GTTGTCGCCT 
TTGCAAAGCG AGCCTCAAAG TCCTATTTAC GCCTCACCGC CTCTTAGTCC GGTACATGAC 
AGTTCTAATA ATGTTTTCGG TGCCATGAAT ATTTATCAGC CGGTACTGCG ACATAACAAC 
TTTGCAAAAG CGCCCGACAT GCACTTTGCT CGAGACACCC ATATGCGCAT AATTTTGGGC 
AATTCCGGCA GTTCCGCATT CGGAAAGGAC AAAAAAGGCG ACGAGAAGCA AGACAAGGAT 
TCAAATAATA CCAAACAGAG CAATCAAAAA TCGAAGCGAA ATGGTATTGC AACGAATCCC 
GCATTACGAT TGGTGGCGAA TGTGAAGAAC GGGTTCGATT CGGCAGCAGC CAATTTGCGT 
AAGTCGACAT CTGGAGCGAA TCCAGCCTCG AAAACGAACA GTTTTCATTG GCGGAAAGAG 
ACGCCTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109537115 ( XM_019903695.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759254.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X3, mRNA.

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CloneID	ODe40658
Clone ID Related Accession (Same CDS sequence)	XM_019903693.1 , XM_019903694.1 , XM_019903695.1
Accession Version	XM_019903695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCAGTGC ATTTTTAGCA TCGACTGCCG AAACCAAACC TTTATCGGCC 
AGTTTGTATC CGGTAGGCGG AGATTGGACC GATCAGCAAC GAAGATCAAT GGTTTCTTTG 
GGTAATCATA GCTCTAATTC AAAGTCCAGT TCGAATCTGA CGGGCTCAAG CCATTCCAGC 
CGCCATGGCG ATAGGCAGCG AGATACACAA CAGAATACTC AGCCGATGTC TTTCACCCAA 
CAGCTCCAGC AGTGTTCTAG AGGTGGCAAA ACAAGACCGT TGAGTAAACT GTTGTCGCCT 
TTGCAAAGCG AGCCTCAAAG TCCTATTTAC GCCTCACCGC CTCTTAGTCC GGTACATGAC 
AGTTCTAATA ATGTTTTCGG TGCCATGAAT ATTTATCAGC CGGTACTGCG ACATAACAAC 
TTTGCAAAAG CGCCCGACAT GCACTTTGCT CGAGACACCC ATATGCGCAT AATTTTGGGC 
AATTCCGGCA GTTCCGCATT CGGAAAGGAC AAAAAAGGCG ACGAGAAGCA AGACAAGGAT 
TCAAATAATA CCAAACAGAG CAATCAAAAA TCGAAGCGAA ATGGTATTGC AACGAATCCC 
GCATTACGAT TGGTGGCGAA TGTGAAGAAC GGGTTCGATT CGGCAGCAGC CAATTTGCGT 
AAGTCGACAT CTGGAGCGAA TCCAGCCTCG AAAACGAACA GTTTTCATTG GCGGAAAGAG 
ACGCCTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759254.1
CDS	377..5128
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKNTALKQTKDGPLDKKQFKSGLINLLCKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQGCVEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASSAFLASTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903695.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903695.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCAGTGC ATTTTTAGCA TCGACTGCCG AAACCAAACC TTTATCGGCC 
AGTTTGTATC CGGTAGGCGG AGATTGGACC GATCAGCAAC GAAGATCAAT GGTTTCTTTG 
GGTAATCATA GCTCTAATTC AAAGTCCAGT TCGAATCTGA CGGGCTCAAG CCATTCCAGC 
CGCCATGGCG ATAGGCAGCG AGATACACAA CAGAATACTC AGCCGATGTC TTTCACCCAA 
CAGCTCCAGC AGTGTTCTAG AGGTGGCAAA ACAAGACCGT TGAGTAAACT GTTGTCGCCT 
TTGCAAAGCG AGCCTCAAAG TCCTATTTAC GCCTCACCGC CTCTTAGTCC GGTACATGAC 
AGTTCTAATA ATGTTTTCGG TGCCATGAAT ATTTATCAGC CGGTACTGCG ACATAACAAC 
TTTGCAAAAG CGCCCGACAT GCACTTTGCT CGAGACACCC ATATGCGCAT AATTTTGGGC 
AATTCCGGCA GTTCCGCATT CGGAAAGGAC AAAAAAGGCG ACGAGAAGCA AGACAAGGAT 
TCAAATAATA CCAAACAGAG CAATCAAAAA TCGAAGCGAA ATGGTATTGC AACGAATCCC 
GCATTACGAT TGGTGGCGAA TGTGAAGAAC GGGTTCGATT CGGCAGCAGC CAATTTGCGT 
AAGTCGACAT CTGGAGCGAA TCCAGCCTCG AAAACGAACA GTTTTCATTG GCGGAAAGAG 
ACGCCTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109537115 ( XM_019903696.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759255.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X4, mRNA.

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CloneID	ODe40659
Clone ID Related Accession (Same CDS sequence)	XM_019903696.1
Accession Version	XM_019903696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGAAGT GGTTCGTCAA TTACTAGACA GAGGTCTTGA TGAGCAGCAC 
AGAGATAACT CTGGATGGAC GCCCTTGCAT TATGCAGCAT TTGAAGGCCA CCAAGATGTA 
TGTGAAGCGC TATTGGAAGC GGGAGCGAAA ATCGACGAGA CTGACAATGA GGGTAAAGCT 
CCGCTTACTC TAGCGGCTCA AGGAGGCCAC ACTTCGTTAG TTAACCTATT TATGAATAAG 
TATAATGCCC CGGTAGATCA AAGGCCGCAT GACGGAAAAA CTGCTTTAAG ATTGGCGGCT 
CTAGAAGGGC ACTACGACAT AGTACAAATT TTGGTATCAA ACGGCGCTGA TATAGACTCG 
AAAGATGCAG ATGGGAGAAG CACACTGTAT GTTTTGGCTT TGGACAATCG GTTGGCGATG 
TCCAAGTACT TCATACAGCA GGAAGCCGAT GTGGAAACCA GAGACCTGGA AGGTCGAACG 
CCGCTTCATG TTTCCGCTTG GCAGGGACAT ACCGAGATGG TTTCTCTTCT TTTAACCTAT 
GGAAAAGCTC TAGTGGATGC TTGCGATCTA GAAAACCGCA CTGCGTTGCA CTCTGCCAGC 
TGGCAAGGGC ATAGTGATAT CGTTAAGATA CTACTCGAAC ATGGTGCTTT GCCTGATCAT 
ACTTGCAATC AGGGAGCCAC AGCATTAGGA ATTGCTGCAC AAGAAGGCCA CGAGCTTTGT 
GTGGTTGCTC TATTAGAACA CGGGGCTGAT CCGAACCATT CGGATGCTTG CGGGCGAAAC 
GCTCTTCGAG TGGCTGCGAA ATCCGGCCAT CGAGGCGTTG TGAGGCTTTT AGAGGAATAC 
AACGCTAGAT CGCATAAAAT TAACATGTCA GCCCATACGA GTAGTAGTGC GAATTCAATA 
GCGTCCAGTG CATTTTTAGC ATCGACTGCC GAAACCAAAC CTTTATCGGC CAGTTTGTAT 
CCGGTAGGCG GAGATTGGAC CGATCAGCAA CGAAGATCAA TGGTTTCTTT GGGTAATCAT 
AGCTCTAATT CAAAGTCCAG TTCGAATCTG ACGGGCTCAA GCCATTCCAG CCGCCATGGC 
GATAGGCAGC GAGATACACA ACAGAATACT CAGCCGATGT CTTTCACCCA ACAGCTCCAG 
CAGTGTTCTA GAGGTGGCAA AACAAGACCG TTGAGTAAAC TGTTGTCGCC TTTGCAAAGC 
GAGCCTCAAA GTCCTATTTA CGCCTCACCG CCTCTTAGTC CGGTACATGA CAGTTCTAAT 
AATGTTTTCG GTGCCATGAA TATTTATCAG CCGGTACTGC GACATAACAA CTTTGCAAAA 
GCGCCCGACA TGCACTTTGC TCGAGACACC CATATGCGCA TAATTTTGGG CAATTCCGGC 
AGTTCCGCAT TCGGAAAGGA CAAAAAAGGC GACGAGAAGC AAGACAAGGA TTCAAATAAT 
ACCAAACAGA GCAATCAAAA ATCGAAGCGA AATGGTATTG CAACGAATCC CGCATTACGA 
TTGGTGGCGA ATGTGAAGAA CGGGTTCGAT TCGGCAGCAG CCAATTTGCG TAAGTCGACA 
TCTGGAGCGA ATCCAGCCTC GAAAACGAAC AGTTTTCATT GGCGGAAAGA GACGCCTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759255.1
CDS	377..5119
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKNTALKQTKDGPLDKKQFKSGLINLLCKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASSAFLASTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903696.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903696.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGAAGT GGTTCGTCAA TTACTAGACA GAGGTCTTGA TGAGCAGCAC 
AGAGATAACT CTGGATGGAC GCCCTTGCAT TATGCAGCAT TTGAAGGCCA CCAAGATGTA 
TGTGAAGCGC TATTGGAAGC GGGAGCGAAA ATCGACGAGA CTGACAATGA GGGTAAAGCT 
CCGCTTACTC TAGCGGCTCA AGGAGGCCAC ACTTCGTTAG TTAACCTATT TATGAATAAG 
TATAATGCCC CGGTAGATCA AAGGCCGCAT GACGGAAAAA CTGCTTTAAG ATTGGCGGCT 
CTAGAAGGGC ACTACGACAT AGTACAAATT TTGGTATCAA ACGGCGCTGA TATAGACTCG 
AAAGATGCAG ATGGGAGAAG CACACTGTAT GTTTTGGCTT TGGACAATCG GTTGGCGATG 
TCCAAGTACT TCATACAGCA GGAAGCCGAT GTGGAAACCA GAGACCTGGA AGGTCGAACG 
CCGCTTCATG TTTCCGCTTG GCAGGGACAT ACCGAGATGG TTTCTCTTCT TTTAACCTAT 
GGAAAAGCTC TAGTGGATGC TTGCGATCTA GAAAACCGCA CTGCGTTGCA CTCTGCCAGC 
TGGCAAGGGC ATAGTGATAT CGTTAAGATA CTACTCGAAC ATGGTGCTTT GCCTGATCAT 
ACTTGCAATC AGGGAGCCAC AGCATTAGGA ATTGCTGCAC AAGAAGGCCA CGAGCTTTGT 
GTGGTTGCTC TATTAGAACA CGGGGCTGAT CCGAACCATT CGGATGCTTG CGGGCGAAAC 
GCTCTTCGAG TGGCTGCGAA ATCCGGCCAT CGAGGCGTTG TGAGGCTTTT AGAGGAATAC 
AACGCTAGAT CGCATAAAAT TAACATGTCA GCCCATACGA GTAGTAGTGC GAATTCAATA 
GCGTCCAGTG CATTTTTAGC ATCGACTGCC GAAACCAAAC CTTTATCGGC CAGTTTGTAT 
CCGGTAGGCG GAGATTGGAC CGATCAGCAA CGAAGATCAA TGGTTTCTTT GGGTAATCAT 
AGCTCTAATT CAAAGTCCAG TTCGAATCTG ACGGGCTCAA GCCATTCCAG CCGCCATGGC 
GATAGGCAGC GAGATACACA ACAGAATACT CAGCCGATGT CTTTCACCCA ACAGCTCCAG 
CAGTGTTCTA GAGGTGGCAA AACAAGACCG TTGAGTAAAC TGTTGTCGCC TTTGCAAAGC 
GAGCCTCAAA GTCCTATTTA CGCCTCACCG CCTCTTAGTC CGGTACATGA CAGTTCTAAT 
AATGTTTTCG GTGCCATGAA TATTTATCAG CCGGTACTGC GACATAACAA CTTTGCAAAA 
GCGCCCGACA TGCACTTTGC TCGAGACACC CATATGCGCA TAATTTTGGG CAATTCCGGC 
AGTTCCGCAT TCGGAAAGGA CAAAAAAGGC GACGAGAAGC AAGACAAGGA TTCAAATAAT 
ACCAAACAGA GCAATCAAAA ATCGAAGCGA AATGGTATTG CAACGAATCC CGCATTACGA 
TTGGTGGCGA ATGTGAAGAA CGGGTTCGAT TCGGCAGCAG CCAATTTGCG TAAGTCGACA 
TCTGGAGCGA ATCCAGCCTC GAAAACGAAC AGTTTTCATT GGCGGAAAGA GACGCCTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109537115 ( XM_019903697.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759256.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X5, mRNA.

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CloneID	ODe40660
Clone ID Related Accession (Same CDS sequence)	XM_019903697.1
Accession Version	XM_019903697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCACATC GACTGCCGAA ACCAAACCTT TATCGGCCAG TTTGTATCCG 
GTAGGCGGAG ATTGGACCGA TCAGCAACGA AGATCAATGG TTTCTTTGGG TAATCATAGC 
TCTAATTCAA AGTCCAGTTC GAATCTGACG GGCTCAAGCC ATTCCAGCCG CCATGGCGAT 
AGGCAGCGAG ATACACAACA GAATACTCAG CCGATGTCTT TCACCCAACA GCTCCAGCAG 
TGTTCTAGAG GTGGCAAAAC AAGACCGTTG AGTAAACTGT TGTCGCCTTT GCAAAGCGAG 
CCTCAAAGTC CTATTTACGC CTCACCGCCT CTTAGTCCGG TACATGACAG TTCTAATAAT 
GTTTTCGGTG CCATGAATAT TTATCAGCCG GTACTGCGAC ATAACAACTT TGCAAAAGCG 
CCCGACATGC ACTTTGCTCG AGACACCCAT ATGCGCATAA TTTTGGGCAA TTCCGGCAGT 
TCCGCATTCG GAAAGGACAA AAAAGGCGAC GAGAAGCAAG ACAAGGATTC AAATAATACC 
AAACAGAGCA ATCAAAAATC GAAGCGAAAT GGTATTGCAA CGAATCCCGC ATTACGATTG 
GTGGCGAATG TGAAGAACGG GTTCGATTCG GCAGCAGCCA ATTTGCGTAA GTCGACATCT 
GGAGCGAATC CAGCCTCGAA AACGAACAGT TTTCATTGGC GGAAAGAGAC GCCTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759256.1
CDS	377..5116
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKNTALKQTKDGPLDKKQFKSGLINLLCKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQGCVEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASTSTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903697.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903697.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG AATACGGCAC TAAAGCAAAC GAAGGACGGT 
CCTTTAGATA AAAAGCAGTT CAAATCCGGC CTAATCAATT TGCTTTGTAA GGTACTACTG 
GAATCAGGCG CCAATGCTGA TCAGGCAGAT ATTGATGGCT GGACGCCGTT GAGGGCAGCG 
GCATGGGGAG GACATACGGA GGTCGTTCAG CTCTTGGTTC AACATGGGTG CCAATTGGAC 
AGCGTTGATG CGGAGAATCG AACAGCTTTG AGGGCGGCAG CCTGGAGTGG CCATGAAGAA 
ATAGTGAAAA TTCTGTTGCA GCATGGAGCC AATGTAAATT TGACGGATCA TGAAGGTCGA 
ACTGCACTAA TAGCTGCAGC CTACATGGGG CACAGCGAAA TTGTGGATCA TCTCCTTGAT 
TTTGGAGCGG ATATCAACCA TCCAGACGCT GATGGCAGAA CCGCTCTATC AGTGGCTGCT 
TTATGCGCCC CACGAACTCC AGGTGTTAGT GTTGTCTCCA CCCTGTTGGA GAGAAATGCT 
ACCGTCGATC ACAAGGACAA GGAGGGAATG ACCCCCTTGT TAGTTGCTTC ATTTGAGGGA 
CACAGGGATG TTTGTGAACT ATTATTAGAG AACGAAGCTG ATGTAGATCA CTGTGATAAC 
AGTGGAAGAT CGCCATTATG GGCAGCGGCC AGTATGGGAC ATGCTCCGGT CGTAGCCTTG 
CTACTGTTTT GGGGCTGCGC CATAGACTCG ATGGATTCGG AAGGACGAAC GGTGCTTTCT 
GTCGCCGCTG CACAAGGTTG TGTAGAAGTG GTTCGTCAAT TACTAGACAG AGGTCTTGAT 
GAGCAGCACA GAGATAACTC TGGATGGACG CCCTTGCATT ATGCAGCATT TGAAGGCCAC 
CAAGATGTAT GTGAAGCGCT ATTGGAAGCG GGAGCGAAAA TCGACGAGAC TGACAATGAG 
GGTAAAGCTC CGCTTACTCT AGCGGCTCAA GGAGGCCACA CTTCGTTAGT TAACCTATTT 
ATGAATAAGT ATAATGCCCC GGTAGATCAA AGGCCGCATG ACGGAAAAAC TGCTTTAAGA 
TTGGCGGCTC TAGAAGGGCA CTACGACATA GTACAAATTT TGGTATCAAA CGGCGCTGAT 
ATAGACTCGA AAGATGCAGA TGGGAGAAGC ACACTGTATG TTTTGGCTTT GGACAATCGG 
TTGGCGATGT CCAAGTACTT CATACAGCAG GAAGCCGATG TGGAAACCAG AGACCTGGAA 
GGTCGAACGC CGCTTCATGT TTCCGCTTGG CAGGGACATA CCGAGATGGT TTCTCTTCTT 
TTAACCTATG GAAAAGCTCT AGTGGATGCT TGCGATCTAG AAAACCGCAC TGCGTTGCAC 
TCTGCCAGCT GGCAAGGGCA TAGTGATATC GTTAAGATAC TACTCGAACA TGGTGCTTTG 
CCTGATCATA CTTGCAATCA GGGAGCCACA GCATTAGGAA TTGCTGCACA AGAAGGCCAC 
GAGCTTTGTG TGGTTGCTCT ATTAGAACAC GGGGCTGATC CGAACCATTC GGATGCTTGC 
GGGCGAAACG CTCTTCGAGT GGCTGCGAAA TCCGGCCATC GAGGCGTTGT GAGGCTTTTA 
GAGGAATACA ACGCTAGATC GCATAAAATT AACATGTCAG CCCATACGAG TAGTAGTGCG 
AATTCAATAG CGTCCACATC GACTGCCGAA ACCAAACCTT TATCGGCCAG TTTGTATCCG 
GTAGGCGGAG ATTGGACCGA TCAGCAACGA AGATCAATGG TTTCTTTGGG TAATCATAGC 
TCTAATTCAA AGTCCAGTTC GAATCTGACG GGCTCAAGCC ATTCCAGCCG CCATGGCGAT 
AGGCAGCGAG ATACACAACA GAATACTCAG CCGATGTCTT TCACCCAACA GCTCCAGCAG 
TGTTCTAGAG GTGGCAAAAC AAGACCGTTG AGTAAACTGT TGTCGCCTTT GCAAAGCGAG 
CCTCAAAGTC CTATTTACGC CTCACCGCCT CTTAGTCCGG TACATGACAG TTCTAATAAT 
GTTTTCGGTG CCATGAATAT TTATCAGCCG GTACTGCGAC ATAACAACTT TGCAAAAGCG 
CCCGACATGC ACTTTGCTCG AGACACCCAT ATGCGCATAA TTTTGGGCAA TTCCGGCAGT 
TCCGCATTCG GAAAGGACAA AAAAGGCGAC GAGAAGCAAG ACAAGGATTC AAATAATACC 
AAACAGAGCA ATCAAAAATC GAAGCGAAAT GGTATTGCAA CGAATCCCGC ATTACGATTG 
GTGGCGAATG TGAAGAACGG GTTCGATTCG GCAGCAGCCA ATTTGCGTAA GTCGACATCT 
GGAGCGAATC CAGCCTCGAA AACGAACAGT TTTCATTGGC GGAAAGAGAC GCCTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109537115 ( XM_019903698.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759257.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X6, mRNA.

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CloneID	ODe40661
Clone ID Related Accession (Same CDS sequence)	XM_019903698.1
Accession Version	XM_019903698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG GTACTACTGG AATCAGGCGC CAATGCTGAT 
CAGGCAGATA TTGATGGCTG GACGCCGTTG AGGGCAGCGG CATGGGGAGG ACATACGGAG 
GTCGTTCAGC TCTTGGTTCA ACATGGGTGC CAATTGGACA GCGTTGATGC GGAGAATCGA 
ACAGCTTTGA GGGCGGCAGC CTGGAGTGGC CATGAAGAAA TAGTGAAAAT TCTGTTGCAG 
CATGGAGCCA ATGTAAATTT GACGGATCAT GAAGGTCGAA CTGCACTAAT AGCTGCAGCC 
TACATGGGGC ACAGCGAAAT TGTGGATCAT CTCCTTGATT TTGGAGCGGA TATCAACCAT 
CCAGACGCTG ATGGCAGAAC CGCTCTATCA GTGGCTGCTT TATGCGCCCC ACGAACTCCA 
GGTGTTAGTG TTGTCTCCAC CCTGTTGGAG AGAAATGCTA CCGTCGATCA CAAGGACAAG 
GAGGGAATGA CCCCCTTGTT AGTTGCTTCA TTTGAGGGAC ACAGGGATGT TTGTGAACTA 
TTATTAGAGA ACGAAGCTGA TGTAGATCAC TGTGATAACA GTGGAAGATC GCCATTATGG 
GCAGCGGCCA GTATGGGACA TGCTCCGGTC GTAGCCTTGC TACTGTTTTG GGGCTGCGCC 
ATAGACTCGA TGGATTCGGA AGGACGAACG GTGCTTTCTG TCGCCGCTGC ACAAGGTTGT 
GTAGAAGTGG TTCGTCAATT ACTAGACAGA GGTCTTGATG AGCAGCACAG AGATAACTCT 
GGATGGACGC CCTTGCATTA TGCAGCATTT GAAGGCCACC AAGATGTATG TGAAGCGCTA 
TTGGAAGCGG GAGCGAAAAT CGACGAGACT GACAATGAGG GTAAAGCTCC GCTTACTCTA 
GCGGCTCAAG GAGGCCACAC TTCGTTAGTT AACCTATTTA TGAATAAGTA TAATGCCCCG 
GTAGATCAAA GGCCGCATGA CGGAAAAACT GCTTTAAGAT TGGCGGCTCT AGAAGGGCAC 
TACGACATAG TACAAATTTT GGTATCAAAC GGCGCTGATA TAGACTCGAA AGATGCAGAT 
GGGAGAAGCA CACTGTATGT TTTGGCTTTG GACAATCGGT TGGCGATGTC CAAGTACTTC 
ATACAGCAGG AAGCCGATGT GGAAACCAGA GACCTGGAAG GTCGAACGCC GCTTCATGTT 
TCCGCTTGGC AGGGACATAC CGAGATGGTT TCTCTTCTTT TAACCTATGG AAAAGCTCTA 
GTGGATGCTT GCGATCTAGA AAACCGCACT GCGTTGCACT CTGCCAGCTG GCAAGGGCAT 
AGTGATATCG TTAAGATACT ACTCGAACAT GGTGCTTTGC CTGATCATAC TTGCAATCAG 
GGAGCCACAG CATTAGGAAT TGCTGCACAA GAAGGCCACG AGCTTTGTGT GGTTGCTCTA 
TTAGAACACG GGGCTGATCC GAACCATTCG GATGCTTGCG GGCGAAACGC TCTTCGAGTG 
GCTGCGAAAT CCGGCCATCG AGGCGTTGTG AGGCTTTTAG AGGAATACAA CGCTAGATCG 
CATAAAATTA ACATGTCAGC CCATACGAGT AGTAGTGCGA ATTCAATAGC GTCCAGTGCA 
TTTTTAGCAT CGACTGCCGA AACCAAACCT TTATCGGCCA GTTTGTATCC GGTAGGCGGA 
GATTGGACCG ATCAGCAACG AAGATCAATG GTTTCTTTGG GTAATCATAG CTCTAATTCA 
AAGTCCAGTT CGAATCTGAC GGGCTCAAGC CATTCCAGCC GCCATGGCGA TAGGCAGCGA 
GATACACAAC AGAATACTCA GCCGATGTCT TTCACCCAAC AGCTCCAGCA GTGTTCTAGA 
GGTGGCAAAA CAAGACCGTT GAGTAAACTG TTGTCGCCTT TGCAAAGCGA GCCTCAAAGT 
CCTATTTACG CCTCACCGCC TCTTAGTCCG GTACATGACA GTTCTAATAA TGTTTTCGGT 
GCCATGAATA TTTATCAGCC GGTACTGCGA CATAACAACT TTGCAAAAGC GCCCGACATG 
CACTTTGCTC GAGACACCCA TATGCGCATA ATTTTGGGCA ATTCCGGCAG TTCCGCATTC 
GGAAAGGACA AAAAAGGCGA CGAGAAGCAA GACAAGGATT CAAATAATAC CAAACAGAGC 
AATCAAAAAT CGAAGCGAAA TGGTATTGCA ACGAATCCCG CATTACGATT GGTGGCGAAT 
GTGAAGAACG GGTTCGATTC GGCAGCAGCC AATTTGCGTA AGTCGACATC TGGAGCGAAT 
CCAGCCTCGA AAACGAACAG TTTTCATTGG CGGAAAGAGA CGCCTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759257.1
CDS	377..5047
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQGCVEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASSAFLASTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903698.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903698.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG GTACTACTGG AATCAGGCGC CAATGCTGAT 
CAGGCAGATA TTGATGGCTG GACGCCGTTG AGGGCAGCGG CATGGGGAGG ACATACGGAG 
GTCGTTCAGC TCTTGGTTCA ACATGGGTGC CAATTGGACA GCGTTGATGC GGAGAATCGA 
ACAGCTTTGA GGGCGGCAGC CTGGAGTGGC CATGAAGAAA TAGTGAAAAT TCTGTTGCAG 
CATGGAGCCA ATGTAAATTT GACGGATCAT GAAGGTCGAA CTGCACTAAT AGCTGCAGCC 
TACATGGGGC ACAGCGAAAT TGTGGATCAT CTCCTTGATT TTGGAGCGGA TATCAACCAT 
CCAGACGCTG ATGGCAGAAC CGCTCTATCA GTGGCTGCTT TATGCGCCCC ACGAACTCCA 
GGTGTTAGTG TTGTCTCCAC CCTGTTGGAG AGAAATGCTA CCGTCGATCA CAAGGACAAG 
GAGGGAATGA CCCCCTTGTT AGTTGCTTCA TTTGAGGGAC ACAGGGATGT TTGTGAACTA 
TTATTAGAGA ACGAAGCTGA TGTAGATCAC TGTGATAACA GTGGAAGATC GCCATTATGG 
GCAGCGGCCA GTATGGGACA TGCTCCGGTC GTAGCCTTGC TACTGTTTTG GGGCTGCGCC 
ATAGACTCGA TGGATTCGGA AGGACGAACG GTGCTTTCTG TCGCCGCTGC ACAAGGTTGT 
GTAGAAGTGG TTCGTCAATT ACTAGACAGA GGTCTTGATG AGCAGCACAG AGATAACTCT 
GGATGGACGC CCTTGCATTA TGCAGCATTT GAAGGCCACC AAGATGTATG TGAAGCGCTA 
TTGGAAGCGG GAGCGAAAAT CGACGAGACT GACAATGAGG GTAAAGCTCC GCTTACTCTA 
GCGGCTCAAG GAGGCCACAC TTCGTTAGTT AACCTATTTA TGAATAAGTA TAATGCCCCG 
GTAGATCAAA GGCCGCATGA CGGAAAAACT GCTTTAAGAT TGGCGGCTCT AGAAGGGCAC 
TACGACATAG TACAAATTTT GGTATCAAAC GGCGCTGATA TAGACTCGAA AGATGCAGAT 
GGGAGAAGCA CACTGTATGT TTTGGCTTTG GACAATCGGT TGGCGATGTC CAAGTACTTC 
ATACAGCAGG AAGCCGATGT GGAAACCAGA GACCTGGAAG GTCGAACGCC GCTTCATGTT 
TCCGCTTGGC AGGGACATAC CGAGATGGTT TCTCTTCTTT TAACCTATGG AAAAGCTCTA 
GTGGATGCTT GCGATCTAGA AAACCGCACT GCGTTGCACT CTGCCAGCTG GCAAGGGCAT 
AGTGATATCG TTAAGATACT ACTCGAACAT GGTGCTTTGC CTGATCATAC TTGCAATCAG 
GGAGCCACAG CATTAGGAAT TGCTGCACAA GAAGGCCACG AGCTTTGTGT GGTTGCTCTA 
TTAGAACACG GGGCTGATCC GAACCATTCG GATGCTTGCG GGCGAAACGC TCTTCGAGTG 
GCTGCGAAAT CCGGCCATCG AGGCGTTGTG AGGCTTTTAG AGGAATACAA CGCTAGATCG 
CATAAAATTA ACATGTCAGC CCATACGAGT AGTAGTGCGA ATTCAATAGC GTCCAGTGCA 
TTTTTAGCAT CGACTGCCGA AACCAAACCT TTATCGGCCA GTTTGTATCC GGTAGGCGGA 
GATTGGACCG ATCAGCAACG AAGATCAATG GTTTCTTTGG GTAATCATAG CTCTAATTCA 
AAGTCCAGTT CGAATCTGAC GGGCTCAAGC CATTCCAGCC GCCATGGCGA TAGGCAGCGA 
GATACACAAC AGAATACTCA GCCGATGTCT TTCACCCAAC AGCTCCAGCA GTGTTCTAGA 
GGTGGCAAAA CAAGACCGTT GAGTAAACTG TTGTCGCCTT TGCAAAGCGA GCCTCAAAGT 
CCTATTTACG CCTCACCGCC TCTTAGTCCG GTACATGACA GTTCTAATAA TGTTTTCGGT 
GCCATGAATA TTTATCAGCC GGTACTGCGA CATAACAACT TTGCAAAAGC GCCCGACATG 
CACTTTGCTC GAGACACCCA TATGCGCATA ATTTTGGGCA ATTCCGGCAG TTCCGCATTC 
GGAAAGGACA AAAAAGGCGA CGAGAAGCAA GACAAGGATT CAAATAATAC CAAACAGAGC 
AATCAAAAAT CGAAGCGAAA TGGTATTGCA ACGAATCCCG CATTACGATT GGTGGCGAAT 
GTGAAGAACG GGTTCGATTC GGCAGCAGCC AATTTGCGTA AGTCGACATC TGGAGCGAAT 
CCAGCCTCGA AAACGAACAG TTTTCATTGG CGGAAAGAGA CGCCTTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109537115 ( XM_019903699.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019759258.1 Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X7, mRNA.

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CloneID	ODe40662
Clone ID Related Accession (Same CDS sequence)	XM_019903699.1
Accession Version	XM_019903699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	ankyrin repeat domain-containing protein 50 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG GTACTACTGG AATCAGGCGC CAATGCTGAT 
CAGGCAGATA TTGATGGCTG GACGCCGTTG AGGGCAGCGG CATGGGGAGG ACATACGGAG 
GTCGTTCAGC TCTTGGTTCA ACATGGGTGC CAATTGGACA GCGTTGATGC GGAGAATCGA 
ACAGCTTTGA GGGCGGCAGC CTGGAGTGGC CATGAAGAAA TAGTGAAAAT TCTGTTGCAG 
CATGGAGCCA ATGTAAATTT GACGGATCAT GAAGGTCGAA CTGCACTAAT AGCTGCAGCC 
TACATGGGGC ACAGCGAAAT TGTGGATCAT CTCCTTGATT TTGGAGCGGA TATCAACCAT 
CCAGACGCTG ATGGCAGAAC CGCTCTATCA GTGGCTGCTT TATGCGCCCC ACGAACTCCA 
GGTGTTAGTG TTGTCTCCAC CCTGTTGGAG AGAAATGCTA CCGTCGATCA CAAGGACAAG 
GAGGGAATGA CCCCCTTGTT AGTTGCTTCA TTTGAGGGAC ACAGGGATGT TTGTGAACTA 
TTATTAGAGA ACGAAGCTGA TGTAGATCAC TGTGATAACA GTGGAAGATC GCCATTATGG 
GCAGCGGCCA GTATGGGACA TGCTCCGGTC GTAGCCTTGC TACTGTTTTG GGGCTGCGCC 
ATAGACTCGA TGGATTCGGA AGGACGAACG GTGCTTTCTG TCGCCGCTGC ACAAGGTTGT 
GTAGAAGTGG TTCGTCAATT ACTAGACAGA GGTCTTGATG AGCAGCACAG AGATAACTCT 
GGATGGACGC CCTTGCATTA TGCAGCATTT GAAGGCCACC AAGATGTATG TGAAGCGCTA 
TTGGAAGCGG GAGCGAAAAT CGACGAGACT GACAATGAGG GTAAAGCTCC GCTTACTCTA 
GCGGCTCAAG GAGGCCACAC TTCGTTAGTT AACCTATTTA TGAATAAGTA TAATGCCCCG 
GTAGATCAAA GGCCGCATGA CGGAAAAACT GCTTTAAGAT TGGCGGCTCT AGAAGGGCAC 
TACGACATAG TACAAATTTT GGTATCAAAC GGCGCTGATA TAGACTCGAA AGATGCAGAT 
GGGAGAAGCA CACTGTATGT TTTGGCTTTG GACAATCGGT TGGCGATGTC CAAGTACTTC 
ATACAGCAGG AAGCCGATGT GGAAACCAGA GACCTGGAAG GTCGAACGCC GCTTCATGTT 
TCCGCTTGGC AGGGACATAC CGAGATGGTT TCTCTTCTTT TAACCTATGG AAAAGCTCTA 
GTGGATGCTT GCGATCTAGA AAACCGCACT GCGTTGCACT CTGCCAGCTG GCAAGGGCAT 
AGTGATATCG TTAAGATACT ACTCGAACAT GGTGCTTTGC CTGATCATAC TTGCAATCAG 
GGAGCCACAG CATTAGGAAT TGCTGCACAA GAAGGCCACG AGCTTTGTGT GGTTGCTCTA 
TTAGAACACG GGGCTGATCC GAACCATTCG GATGCTTGCG GGCGAAACGC TCTTCGAGTG 
GCTGCGAAAT CCGGCCATCG AGGCGTTGTG AGGCTTTTAG AGGAATACAA CGCTAGATCG 
CATAAAATTA ACATGTCAGC CCATACGAGT AGTAGTGCGA ATTCAATAGC GTCCACATCG 
ACTGCCGAAA CCAAACCTTT ATCGGCCAGT TTGTATCCGG TAGGCGGAGA TTGGACCGAT 
CAGCAACGAA GATCAATGGT TTCTTTGGGT AATCATAGCT CTAATTCAAA GTCCAGTTCG 
AATCTGACGG GCTCAAGCCA TTCCAGCCGC CATGGCGATA GGCAGCGAGA TACACAACAG 
AATACTCAGC CGATGTCTTT CACCCAACAG CTCCAGCAGT GTTCTAGAGG TGGCAAAACA 
AGACCGTTGA GTAAACTGTT GTCGCCTTTG CAAAGCGAGC CTCAAAGTCC TATTTACGCC 
TCACCGCCTC TTAGTCCGGT ACATGACAGT TCTAATAATG TTTTCGGTGC CATGAATATT 
TATCAGCCGG TACTGCGACA TAACAACTTT GCAAAAGCGC CCGACATGCA CTTTGCTCGA 
GACACCCATA TGCGCATAAT TTTGGGCAAT TCCGGCAGTT CCGCATTCGG AAAGGACAAA 
AAAGGCGACG AGAAGCAAGA CAAGGATTCA AATAATACCA AACAGAGCAA TCAAAAATCG 
AAGCGAAATG GTATTGCAAC GAATCCCGCA TTACGATTGG TGGCGAATGT GAAGAACGGG 
TTCGATTCGG CAGCAGCCAA TTTGCGTAAG TCGACATCTG GAGCGAATCC AGCCTCGAAA 
ACGAACAGTT TTCATTGGCG GAAAGAGACG CCTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019759258.1
CDS	377..5035
Translation	MAEDRKKFYCREWAFQKLLHCLDQRPVSKTCGALLLGSSGCGKTTFCSELCWPSQGPSGRQQRTLNRRLLCYHFLQGHNEKSLSLSEFVRSLVTQILNHSTESHKERSMRRKIAQAKEQGETVSPKNTSYDKNLSENCNLPQESLYSNVYSENLLSPTLEAKKVPDPAPIPNMPINPRTVIADAYLEKLKSDPEIQIALRAKNIESNPDDCLKRALLFPLLEIDPPKTSLFIIIDSIDEHSFIYSNSTLTRSKPRERSSQSRTIAELLANHHHLFPQWLLnullVCTAKKQSKSIAKMFTGFRKLSLDDLRKAQVVRDVQQYILARLDQENSLRQHISRDTAEMLNQLHIKSNGCFLYLEKVLDGVAESFIVLREVREIPGTLNGLYLWLCQRLFNRRQFAKVQPLLNVILAAKCALNESLLFDIVKVNNSKITIEDFRKRFHLLKKVVVISKNGTIRLFHHSFAEWLLDIKHCTQKYLCNIFHGHCILAMYYTLHAKVLNNQEIYDYAFHLTRMEQFLNEKPSQKCPTFMYKLSQEKAGNSLDKTSTEIYSDLKALQELTKSYENELAYELNNIENLESSFADTETSQNNLDDVNFETKLEEHFDSVHLKSPESEKNPIYAEVNKLKKTSNQITKSVEVSNQLMTTSCENNLLVTDYTNTASSLNNTATSCTMLEGALSKTQQHPILDLHTLTVLWLINSGCNVEECLLEGNEMAGVNFGAFLPTDQKVLRLLLEAGAVEQHDVAGCDYESQMSLASTSSEQSPEPLFDLQDLHGQAALHVAARLGQAQVVKVLLESGANADQADIDGWTPLRAAAWGGHTEVVQLLVQHGCQLDSVDAENRTALRAAAWSGHEEIVKILLQHGANVNLTDHEGRTALIAAAYMGHSEIVDHLLDFGADINHPDADGRTALSVAALCAPRTPGVSVVSTLLERNATVDHKDKEGMTPLLVASFEGHRDVCELLLENEADVDHCDNSGRSPLWAAASMGHAPVVALLLFWGCAIDSMDSEGRTVLSVAAAQGCVEVVRQLLDRGLDEQHRDNSGWTPLHYAAFEGHQDVCEALLEAGAKIDETDNEGKAPLTLAAQGGHTSLVNLFMNKYNAPVDQRPHDGKTALRLAALEGHYDIVQILVSNGADIDSKDADGRSTLYVLALDNRLAMSKYFIQQEADVETRDLEGRTPLHVSAWQGHTEMVSLLLTYGKALVDACDLENRTALHSASWQGHSDIVKILLEHGALPDHTCNQGATALGIAAQEGHELCVVALLEHGADPNHSDACGRNALRVAAKSGHRGVVRLLEEYNARSHKINMSAHTSSSANSIASTSTAETKPLSASLYPVGGDWTDQQRRSMVSLGNHSSNSKSSSNLTGSSHSSRHGDRQRDTQQNTQPMSFTQQLQQCSRGGKTRPLSKLLSPLQSEPQSPIYASPPLSPVHDSSNNVFGAMNIYQPVLRHNNFAKAPDMHFARDTHMRIILGNSGSSAFGKDKKGDEKQDKDSNNTKQSNQKSKRNGIATNPALRLVANVKNGFDSAAANLRKSTSGANPASKTNSFHWRKETPL

Target ORF information:

RefSeq Version	XM_019903699.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae ankyrin repeat domain-containing protein 50 (LOC109537115), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019903699.1

ORF Insert Sequence:

ATGGCGGAAG ATAGAAAGAA GTTTTACTGT CGGGAGTGGG CCTTTCAGAA GCTTCTCCAT 
TGTCTGGATC AAAGGCCAGT GTCAAAGACA TGCGGAGCTC TTTTGTTGGG AAGTTCTGGA 
TGTGGAAAGA CCACGTTTTG TTCAGAACTT TGCTGGCCTT CACAAGGACC TTCAGGCCGA 
CAGCAACGGA CCTTGAATCG TCGATTGCTG TGTTATCACT TCTTGCAAGG CCATAATGAG 
AAAAGTCTGT CGTTGAGTGA ATTTGTGCGA TCATTGGTTA CGCAAATTTT GAATCACTCG 
ACCGAAAGTC ATAAAGAGCG AAGCATGAGG AGGAAAATAG CGCAGGCCAA AGAACAAGGT 
GAAACAGTGA GCCCAAAGAA TACTTCCTAT GATAAAAATC TGTCTGAGAA CTGCAACCTG 
CCGCAGGAGA GTCTTTATAG TAATGTTTAC TCGGAAAATC TCCTGTCCCC GACATTGGAA 
GCCAAAAAAG TGCCTGATCC AGCTCCAATT CCTAATATGC CAATTAATCC TCGCACAGTA 
ATTGCAGATG CGTATTTGGA AAAGCTGAAA TCAGATCCGG AGATTCAGAT CGCTCTGCGA 
GCGAAGAACA TTGAAAGCAA TCCGGACGAT TGTCTGAAAA GAGCGTTACT ATTCCCCCTT 
CTGGAAATAG ATCCTCCTAA AACGTCGTTG TTTATAATCA TTGATTCCAT TGACGAGCAT 
TCGTTTATTT ATTCTAACTC CACCCTAACG CGCTCTAAAC CTCGCGAAAG AAGCTCACAA 
TCACGCACGA TAGCCGAGTT ACTTGCCAAC CATCACCATC TATTCCCGCA ATGGCTTTTG 
NTGGTTTGCA CAGCAAAGAA ACAATCCAAA AGCATTGCCA AGATGTTTAC TGGGTTTAGA 
AAACTGAGTT TGGATGACCT CAGGAAAGCT CAAGTGGTTC GAGACGTTCA GCAATACATT 
CTAGCCAGGC TGGACCAGGA GAATAGCTTA AGACAGCACA TAAGTAGAGA CACGGCCGAA 
ATGCTTAATC AACTACATAT TAAGAGCAAC GGATGTTTTT TATATCTCGA GAAAGTATTG 
GACGGCGTAG CAGAGAGTTT TATCGTTTTG AGGGAAGTTA GAGAAATCCC TGGAACGTTA 
AATGGCTTGT ACTTGTGGCT GTGCCAGAGG TTGTTTAACA GAAGACAATT TGCTAAAGTC 
CAACCACTCT TAAACGTGAT TCTAGCCGCT AAATGTGCTC TAAACGAGTC CCTATTGTTT 
GATATCGTGA AGGTAAACAA CTCCAAGATT ACCATAGAAG ATTTCCGCAA AAGGTTCCAT 
TTGCTGAAGA AAGTCGTTGT GATCTCCAAG AATGGCACAA TTCGACTGTT TCACCATTCG 
TTTGCCGAGT GGCTTTTAGA TATAAAACAT TGCACGCAAA AATACCTATG CAACATTTTC 
CACGGACACT GCATTCTTGC CATGTATTAC ACTCTTCACG CCAAGGTGCT GAACAATCAA 
GAAATCTACG ATTACGCCTT TCATCTCACA CGAATGGAAC AGTTTTTAAA CGAAAAGCCT 
AGTCAGAAGT GCCCTACGTT CATGTATAAG CTGTCGCAGG AGAAAGCTGG CAATTCCCTA 
GATAAAACTT CCACTGAGAT TTATTCGGAT TTGAAAGCCT TGCAGGAATT GACCAAATCG 
TATGAAAACG AATTGGCTTA TGAGCTGAAT AATATTGAAA ACTTGGAAAG TAGCTTTGCG 
GATACGGAAA CTAGTCAGAA TAACTTGGAT GATGTTAATT TCGAGACCAA GTTGGAGGAG 
CACTTTGACA GCGTCCATTT GAAGAGTCCG GAGTCGGAGA AGAATCCGAT TTATGCTGAA 
GTGAATAAGC TAAAGAAAAC ATCAAACCAG ATTACCAAAA GCGTTGAGGT ATCGAATCAG 
CTAATGACCA CCAGCTGCGA AAACAATCTC CTAGTCACCG ATTATACAAA CACAGCTTCG 
TCGCTCAACA ACACAGCTAC TTCTTGCACT ATGTTGGAGG GCGCTCTGTC CAAGACTCAG 
CAACATCCCA TACTGGACTT GCATACGCTA ACCGTTTTGT GGCTGATAAA CAGCGGTTGT 
AATGTGGAGG AATGTTTGCT GGAAGGAAAT GAAATGGCCG GTGTGAATTT CGGAGCGTTT 
TTGCCAACCG ATCAGAAAGT GCTGAGATTG CTGTTGGAAG CTGGTGCTGT TGAGCAGCAT 
GATGTTGCTG GCTGCGATTA CGAAAGTCAA ATGTCATTGG CAAGCACATC CAGCGAACAA 
TCGCCAGAAC CATTATTCGA CTTGCAAGAT CTTCATGGGC AAGCGGCTCT CCATGTTGCG 
GCCAGATTAG GACAAGCTCA AGTCGTTAAG GTACTACTGG AATCAGGCGC CAATGCTGAT 
CAGGCAGATA TTGATGGCTG GACGCCGTTG AGGGCAGCGG CATGGGGAGG ACATACGGAG 
GTCGTTCAGC TCTTGGTTCA ACATGGGTGC CAATTGGACA GCGTTGATGC GGAGAATCGA 
ACAGCTTTGA GGGCGGCAGC CTGGAGTGGC CATGAAGAAA TAGTGAAAAT TCTGTTGCAG 
CATGGAGCCA ATGTAAATTT GACGGATCAT GAAGGTCGAA CTGCACTAAT AGCTGCAGCC 
TACATGGGGC ACAGCGAAAT TGTGGATCAT CTCCTTGATT TTGGAGCGGA TATCAACCAT 
CCAGACGCTG ATGGCAGAAC CGCTCTATCA GTGGCTGCTT TATGCGCCCC ACGAACTCCA 
GGTGTTAGTG TTGTCTCCAC CCTGTTGGAG AGAAATGCTA CCGTCGATCA CAAGGACAAG 
GAGGGAATGA CCCCCTTGTT AGTTGCTTCA TTTGAGGGAC ACAGGGATGT TTGTGAACTA 
TTATTAGAGA ACGAAGCTGA TGTAGATCAC TGTGATAACA GTGGAAGATC GCCATTATGG 
GCAGCGGCCA GTATGGGACA TGCTCCGGTC GTAGCCTTGC TACTGTTTTG GGGCTGCGCC 
ATAGACTCGA TGGATTCGGA AGGACGAACG GTGCTTTCTG TCGCCGCTGC ACAAGGTTGT 
GTAGAAGTGG TTCGTCAATT ACTAGACAGA GGTCTTGATG AGCAGCACAG AGATAACTCT 
GGATGGACGC CCTTGCATTA TGCAGCATTT GAAGGCCACC AAGATGTATG TGAAGCGCTA 
TTGGAAGCGG GAGCGAAAAT CGACGAGACT GACAATGAGG GTAAAGCTCC GCTTACTCTA 
GCGGCTCAAG GAGGCCACAC TTCGTTAGTT AACCTATTTA TGAATAAGTA TAATGCCCCG 
GTAGATCAAA GGCCGCATGA CGGAAAAACT GCTTTAAGAT TGGCGGCTCT AGAAGGGCAC 
TACGACATAG TACAAATTTT GGTATCAAAC GGCGCTGATA TAGACTCGAA AGATGCAGAT 
GGGAGAAGCA CACTGTATGT TTTGGCTTTG GACAATCGGT TGGCGATGTC CAAGTACTTC 
ATACAGCAGG AAGCCGATGT GGAAACCAGA GACCTGGAAG GTCGAACGCC GCTTCATGTT 
TCCGCTTGGC AGGGACATAC CGAGATGGTT TCTCTTCTTT TAACCTATGG AAAAGCTCTA 
GTGGATGCTT GCGATCTAGA AAACCGCACT GCGTTGCACT CTGCCAGCTG GCAAGGGCAT 
AGTGATATCG TTAAGATACT ACTCGAACAT GGTGCTTTGC CTGATCATAC TTGCAATCAG 
GGAGCCACAG CATTAGGAAT TGCTGCACAA GAAGGCCACG AGCTTTGTGT GGTTGCTCTA 
TTAGAACACG GGGCTGATCC GAACCATTCG GATGCTTGCG GGCGAAACGC TCTTCGAGTG 
GCTGCGAAAT CCGGCCATCG AGGCGTTGTG AGGCTTTTAG AGGAATACAA CGCTAGATCG 
CATAAAATTA ACATGTCAGC CCATACGAGT AGTAGTGCGA ATTCAATAGC GTCCACATCG 
ACTGCCGAAA CCAAACCTTT ATCGGCCAGT TTGTATCCGG TAGGCGGAGA TTGGACCGAT 
CAGCAACGAA GATCAATGGT TTCTTTGGGT AATCATAGCT CTAATTCAAA GTCCAGTTCG 
AATCTGACGG GCTCAAGCCA TTCCAGCCGC CATGGCGATA GGCAGCGAGA TACACAACAG 
AATACTCAGC CGATGTCTTT CACCCAACAG CTCCAGCAGT GTTCTAGAGG TGGCAAAACA 
AGACCGTTGA GTAAACTGTT GTCGCCTTTG CAAAGCGAGC CTCAAAGTCC TATTTACGCC 
TCACCGCCTC TTAGTCCGGT ACATGACAGT TCTAATAATG TTTTCGGTGC CATGAATATT 
TATCAGCCGG TACTGCGACA TAACAACTTT GCAAAAGCGC CCGACATGCA CTTTGCTCGA 
GACACCCATA TGCGCATAAT TTTGGGCAAT TCCGGCAGTT CCGCATTCGG AAAGGACAAA 
AAAGGCGACG AGAAGCAAGA CAAGGATTCA AATAATACCA AACAGAGCAA TCAAAAATCG 
AAGCGAAATG GTATTGCAAC GAATCCCGCA TTACGATTGG TGGCGAATGT GAAGAACGGG 
TTCGATTCGG CAGCAGCCAA TTTGCGTAAG TCGACATCTG GAGCGAATCC AGCCTCGAAA 
ACGAACAGTT TTCATTGGCG GAAAGAGACG CCTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

LOC109537115 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム