LOC109533089 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109533089
Entrez Gene ID	109533089
Full Name	probable DNA double-strand break repair Rad50 ATPase
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019898319.1	XP_019753878.1	uncharacterized protein LOC109533089

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis.
Keeling CI, Li M, Sandhu HK, Henderson H, Yuen MM, Bohlmann J
Insect biochemistry and molecular biology70170-83(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC109533089 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109533089 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe36565	XM_019898319.1 Latest version!	Dendroctonus ponderosae probable DNA double-strand break repair Rad50 ATPase (LOC109533089), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109533089 ( XM_019898319.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019753878.1 Dendroctonus ponderosae probable DNA double-strand break repair Rad50 ATPase (LOC109533089), mRNA.

Price & Availability↑

CloneID	ODe36565
Clone ID Related Accession (Same CDS sequence)	XM_019898319.1
Accession Version	XM_019898319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	uncharacterized protein LOC109533089
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851382.1) and transcript sequence (GDAR01007614.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 218 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAGCACA TTCAAGAAAA CCGATTCAAT AGATTGAAAG AGGAGATGGA GCAACTGGAG 
AAGGACTCTG AAGAGCTGAG GGAAGTTAAA ACGAATCTGG AAAAGCAATT GTTTGAGTTG 
CAAGATGATC ACAGTCAAAT GAAGAATCAC ATAACTAGCT TAGAAAATGA ATCAGATGTC 
TTAGAGTCCC AGCTAAAAAT AGTGCTGGAT CAAAAGGAAG AGGATTACGT ATTTTACAAG 
AAACATATTG CGGAAATATC AAAGACTCGG GACGAACTTG AAGAACAAAG CAAAAAAGAA 
ATATCGATGT TGCGTATGGA GTTGCAAGAT CTAAACAACC GACTTGTAAA TGAAAAAACT 
GCATATGAGA CGCTTTCACA AATCCACAAA GATATTTCGG GAAAATTTAT GAGAAGCATT 
AAAGACCTGG TTGAGGCAAA GAATTCCAAG TGTGATATGC AGGATAAGAT GCACAAAAAC 
CAAAAATATC TAGACAATGT TGAAAATGAT CAGAAGCAAT TTCTGAAACA AATTAAAGAC 
CTCACAGAAA AACTTGAATT CTCCCAGACA CACACTAAAA AAATCGAGGA TGAAAAACGT 
ATTCTAGAAA GCAATTATAA CATGACAATT GAACAGTGTC GAAGGCTGGA AGAACAGGTA 
AATGACATTA AAAAGGACCA CCAAACAGAG CATATTCAGT ACAATTCGGA CAAACATAAA 
GAGGACAACA CCTCACAGGA GGAAATGGAA GAAATGAAAT CCGAAATTGA AAGAAAAATT 
GCCACTATTC ATCGGCTTAA AATGGAAGTT TTAGAGCTAC AAAAAGAAAT CACGCTCTTG 
CAGCTGCAAA AGTCCGAAAT AATGTTGCAA CTTCACGAAA CCGAAATAAG ATTGGAGACT 
GAAAAAAACT TGGGTGATCA GTTAAAAAAT ACACAAAAAC TTCTTTTGGT TGCGGTCTTG 
CAACTAAAAG ATGAGGGCAA AATAGATTCA TCAGATTGCA TTCACCTGTT GCATATGGTC 
AGCAGCTCTA GCCCCATAAA TTTAGATATG AAAACTGAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019753878.1
CDS	46..1110
Translation	MEHIQENRFNRLKEEMEQLEKDSEELREVKTNLEKQLFELQDDHSQMKNHITSLENESDVLESQLKIVLDQKEEDYVFYKKHIAEISKTRDELEEQSKKEISMLRMELQDLNNRLVNEKTAYETLSQIHKDISGKFMRSIKDLVEAKNSKCDMQDKMHKNQKYLDNVENDQKQFLKQIKDLTEKLEFSQTHTKKIEDEKRILESNYNMTIEQCRRLEEQVNDIKKDHQTEHIQYNSDKHKEDNTSQEEMEEMKSEIERKIATIHRLKMEVLELQKEITLLQLQKSEIMLQLHETEIRLETEKNLGDQLKNTQKLLLVAVLQLKDEGKIDSSDCIHLLHMVSSSSPINLDMKTEN

Target ORF information:

RefSeq Version	XM_019898319.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae probable DNA double-strand break repair Rad50 ATPase (LOC109533089), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019898319.1

ORF Insert Sequence:

ATGGAGCACA TTCAAGAAAA CCGATTCAAT AGATTGAAAG AGGAGATGGA GCAACTGGAG 
AAGGACTCTG AAGAGCTGAG GGAAGTTAAA ACGAATCTGG AAAAGCAATT GTTTGAGTTG 
CAAGATGATC ACAGTCAAAT GAAGAATCAC ATAACTAGCT TAGAAAATGA ATCAGATGTC 
TTAGAGTCCC AGCTAAAAAT AGTGCTGGAT CAAAAGGAAG AGGATTACGT ATTTTACAAG 
AAACATATTG CGGAAATATC AAAGACTCGG GACGAACTTG AAGAACAAAG CAAAAAAGAA 
ATATCGATGT TGCGTATGGA GTTGCAAGAT CTAAACAACC GACTTGTAAA TGAAAAAACT 
GCATATGAGA CGCTTTCACA AATCCACAAA GATATTTCGG GAAAATTTAT GAGAAGCATT 
AAAGACCTGG TTGAGGCAAA GAATTCCAAG TGTGATATGC AGGATAAGAT GCACAAAAAC 
CAAAAATATC TAGACAATGT TGAAAATGAT CAGAAGCAAT TTCTGAAACA AATTAAAGAC 
CTCACAGAAA AACTTGAATT CTCCCAGACA CACACTAAAA AAATCGAGGA TGAAAAACGT 
ATTCTAGAAA GCAATTATAA CATGACAATT GAACAGTGTC GAAGGCTGGA AGAACAGGTA 
AATGACATTA AAAAGGACCA CCAAACAGAG CATATTCAGT ACAATTCGGA CAAACATAAA 
GAGGACAACA CCTCACAGGA GGAAATGGAA GAAATGAAAT CCGAAATTGA AAGAAAAATT 
GCCACTATTC ATCGGCTTAA AATGGAAGTT TTAGAGCTAC AAAAAGAAAT CACGCTCTTG 
CAGCTGCAAA AGTCCGAAAT AATGTTGCAA CTTCACGAAA CCGAAATAAG ATTGGAGACT 
GAAAAAAACT TGGGTGATCA GTTAAAAAAT ACACAAAAAC TTCTTTTGGT TGCGGTCTTG 
CAACTAAAAG ATGAGGGCAA AATAGATTCA TCAGATTGCA TTCACCTGTT GCATATGGTC 
AGCAGCTCTA GCCCCATAAA TTTAGATATG AAAACTGAAA ATTAA